mRNA_L-elsbetiae_contig17027.4708.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig17027.4708.1
Unique NamemRNA_L-elsbetiae_contig17027.4708.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig17027.4708.1 vs. uniprot
Match: D7FY73_ECTSI (Topoisomerase 6 subunit B n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FY73_ECTSI)

HSP 1 Score: 218 bits (556), Expect = 2.170e-63
Identity = 126/186 (67.74%), Postives = 136/186 (73.12%), Query Frame = 1
Query:   10 KGSLLYGELLPRGVSKAMGPRHLNAAKASTVYDLGMGTGKVAMQVFLQFPNVKRVYGIELSRARFELGEAALFALMELQPGAYAIKKHIPGQIMVIETVAAPKSGSGXXXXXXXXXXXXXXXXPQPSLAEXXXXXXXXXXXXXXXXXXXXXXXGVEGWERICRGAPGRTLEFACGNLFDVENITQA 567
            KGSLLYGELLPRGV+KAMGPRHLNAAKAST+YDLGMGTGKVAMQVFLQFPN+ RVYGIELS+ARFELGE ALFALMELQPGAY IK+HIPGQ + IET+A  +    XXXXXXXX          PSLAE                       G  GWE +CRG PGRTLEFACGNLFDV N+ QA
Sbjct:  303 KGSLLYGELLPRGVNKAMGPRHLNAAKASTIYDLGMGTGKVAMQVFLQFPNLTRVYGIELSQARFELGEEALFALMELQPGAYTIKEHIPGQFISIETLAQDQDHXXXXXXXXXX----------PSLAEGGGWRE-----------------GGAGWEHLCRGPPGRTLEFACGNLFDVTNLAQA 461          
BLAST of mRNA_L-elsbetiae_contig17027.4708.1 vs. uniprot
Match: A0A6H5KB73_9PHAE (DOT1 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KB73_9PHAE)

HSP 1 Score: 217 bits (553), Expect = 4.780e-63
Identity = 119/186 (63.98%), Postives = 128/186 (68.82%), Query Frame = 1
Query:   10 KGSLLYGELLPRGVSKAMGPRHLNAAKASTVYDLGMGTGKVAMQVFLQFPNVKRVYGIELSRARFELGEAALFALMELQPGAYAIKKHIPGQIMVIETVAAPKSGSGXXXXXXXXXXXXXXXXPQPSLAEXXXXXXXXXXXXXXXXXXXXXXXGVEGWERICRGAPGRTLEFACGNLFDVENITQA 567
            KGSLLYGELLPRGV+KAMGPRHLNAAKAST+YDLGMGTGKVAMQVFLQFPN+ RVYGIELS+ARFELGEAALFALMELQPGAYAIKKHIPGQ + IET+                         Q SLAE                       G  GWE +CRG PGRTLEFACGNLFDV ++ QA
Sbjct:  300 KGSLLYGELLPRGVNKAMGPRHLNAAKASTIYDLGMGTGKVAMQVFLQFPNLTRVYGIELSQARFELGEAALFALMELQPGAYAIKKHIPGQFISIETLDQDHKQQ------------------QLSLAEGDGWRE-----------------GGAGWEHLCRGPPGRTLEFACGNLFDVTHLAQA 450          
BLAST of mRNA_L-elsbetiae_contig17027.4708.1 vs. uniprot
Match: A0A7S4DH00_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DH00_HETAK)

HSP 1 Score: 123 bits (308), Expect = 1.080e-29
Identity = 67/110 (60.91%), Postives = 78/110 (70.91%), Query Frame = 1
Query:    1 KQKKGSLLYGELLPRGVSKAMGPRHLNAAKASTVYDLGMGTGKVAMQVFLQFPNVKRVYGIELSRARFELGEAALFALMELQPGAYAIKKHIPGQIMVIETVAAPKSGSG 330
            KQ KGSLLYGELLPRGV+KA+G   LNAA A  VYDLGMG GKVAMQ FLQFP++ RVYG+ELS+AR+EL   AL  L+ L P AY I +  PG+     TV   + G G
Sbjct:  222 KQSKGSLLYGELLPRGVNKALGRGCLNAADARVVYDLGMGLGKVAMQAFLQFPHLARVYGVELSKARYELAALALRRLVALFPEAYCIAEEEPGR----RTVLRARGGGG 327          
BLAST of mRNA_L-elsbetiae_contig17027.4708.1 vs. uniprot
Match: A0A7S3ZW16_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S3ZW16_9STRA)

HSP 1 Score: 117 bits (292), Expect = 1.740e-27
Identity = 60/99 (60.61%), Postives = 73/99 (73.74%), Query Frame = 1
Query:   10 KGSLLYGELLPRGVSKAMGPRHLNAAKASTVYDLGMGTGKVAMQVFLQFPNVKRVYGIELSRARFELGEAALFALMELQPGAYAIKKHIPGQIMVIETV 306
            +GSLLYGELLPRGV KA+  RHL+AA+AS+VYDLG GTGKVA+Q FLQFPN+KRVYG+ELS ARF + E AL AL         I +  PG  + + +V
Sbjct:  177 RGSLLYGELLPRGVHKALDARHLDAARASSVYDLGCGTGKVALQAFLQFPNLKRVYGVELSLARFHVAERALLALAARDDCDAHITRREPGSSIEVTSV 275          
BLAST of mRNA_L-elsbetiae_contig17027.4708.1 vs. uniprot
Match: A0A8J2WX67_9STRA (Hypothetical protein n=2 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2WX67_9STRA)

HSP 1 Score: 117 bits (292), Expect = 7.570e-27
Identity = 60/99 (60.61%), Postives = 73/99 (73.74%), Query Frame = 1
Query:   10 KGSLLYGELLPRGVSKAMGPRHLNAAKASTVYDLGMGTGKVAMQVFLQFPNVKRVYGIELSRARFELGEAALFALMELQPGAYAIKKHIPGQIMVIETV 306
            +GSLLYGELLPRGV KA+  RHL+AA+AS+VYDLG GTGKVA+Q FLQFPN+KRVYG+ELS ARF + E AL AL         I +  PG  + + +V
Sbjct:  177 RGSLLYGELLPRGVHKALDARHLDAARASSVYDLGCGTGKVALQAFLQFPNLKRVYGVELSLARFHVAERALLALAARDDCDAHITRREPGSSIEVTSV 275          
BLAST of mRNA_L-elsbetiae_contig17027.4708.1 vs. uniprot
Match: A0A7S3NGR3_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3NGR3_9STRA)

HSP 1 Score: 107 bits (266), Expect = 1.540e-23
Identity = 51/77 (66.23%), Postives = 63/77 (81.82%), Query Frame = 1
Query:    4 QKKGSLLYGELLPRGVSKAMGPRHLNAAKASTVYDLGMGTGKVAMQVFLQFPNVKRVYGIELSRARFELGEAALFAL 234
            Q +GSLLYGELLPRGV+KA+  RH++A +A+ ++DLGMGTGKV MQ FLQFPN+KRVYG+ELS AR+ L E A   L
Sbjct:  191 QNRGSLLYGELLPRGVNKALDERHMDARRATILFDLGMGTGKVPMQAFLQFPNLKRVYGVELSVARYRLAEKAYLNL 267          
BLAST of mRNA_L-elsbetiae_contig17027.4708.1 vs. uniprot
Match: A0A7S3HRH5_9STRA (Hypothetical protein (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3HRH5_9STRA)

HSP 1 Score: 102 bits (255), Expect = 1.290e-22
Identity = 54/108 (50.00%), Postives = 72/108 (66.67%), Query Frame = 1
Query:   10 KGSLLYGELLPRGVSKAMGPRHLNAAKASTVYDLGMGTGKVAMQVFLQFPNVKRVYGIELSRARFELGEAALFALMELQPGAYAIKKHIPGQ-IMVIETVAAPKSGSG 330
            KGSLLYGELLPRG +KA GP  LN + AS ++DLGMGTGK+ +Q FLQF N++ ++GIELS  R++L E AL  ++ L        +  PG+ I+V E V   + G G
Sbjct:  225 KGSLLYGELLPRGANKAFGPDRLNTSSASVLFDLGMGTGKILIQAFLQFKNLRYIFGIELSAGRYKLAEEALLRMVRLLGEDNYHVQINPGRFIIVTEVVRVSEEGDG 332          
BLAST of mRNA_L-elsbetiae_contig17027.4708.1 vs. uniprot
Match: A0A7S1CCX5_9STRA (Hypothetical protein n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CCX5_9STRA)

HSP 1 Score: 100 bits (250), Expect = 2.200e-22
Identity = 46/101 (45.54%), Postives = 70/101 (69.31%), Query Frame = 1
Query:   16 SLLYGELLPRGVSKAMGPRHLNAAKASTVYDLGMGTGKVAMQVFLQFPNVKRVYGIELSRARFELGEAALFALMELQPGAYAIKKHIPGQIMVIETVAAPK 318
            SLLYGE+L  G++K +   HL+AA+A  ++DLGMG GK+ MQ FLQ+PN+K + G+EL+ +RF+L E AL  L+ LQ   + +++  PG+ + + TV   K
Sbjct:   58 SLLYGEILAVGITKLLDTEHLHAARAQVLFDLGMGVGKLVMQAFLQYPNLKLIRGVELAYSRFKLAEEALLRLVSLQSDRFEVEERTPGEYIRVHTVGRRK 158          
BLAST of mRNA_L-elsbetiae_contig17027.4708.1 vs. uniprot
Match: A0A5A8CLP2_CAFRO (DOT1 domain-containing protein n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CLP2_CAFRO)

HSP 1 Score: 97.4 bits (241), Expect = 1.990e-20
Identity = 50/98 (51.02%), Postives = 67/98 (68.37%), Query Frame = 1
Query:   16 SLLYGELLPRGVSKAMGPRHLNAAKASTVYDLGMGTGKVAMQVFLQFPNVKRVYGIELSRARFELGEAALFALMELQPGAYAIKKHIPGQIMVIETVA 309
            SLLYGELLP GV K M   HL A  A ++ DLGMGTGK+ MQVFL +PN+ RV G+ELSR+R+ + EAA+  L  +Q   + ++   PG+ + I T+A
Sbjct:  173 SLLYGELLPMGVCKLMNQYHLAAGSARSLLDLGMGTGKLVMQVFLLYPNLTRVVGVELSRSRYAIAEAAVLELARIQ--GWEVEARDPGRFVRIRTLA 268          
BLAST of mRNA_L-elsbetiae_contig17027.4708.1 vs. uniprot
Match: A0A7S2SB25_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2SB25_9STRA)

HSP 1 Score: 98.2 bits (243), Expect = 2.770e-20
Identity = 51/103 (49.51%), Postives = 70/103 (67.96%), Query Frame = 1
Query:    1 KQKKGSLLYGELLPRGVSKAMGPRHLNAAKASTVYDLGMGTGKVAMQVFLQFPNVKRVYGIELSRARFELGEAALFALMELQP-GAYAIKKHIPGQIMVIETV 306
            K+ K S+LYGELLP+GV++A+   HL+    S++ DLGMGTGKV +Q FLQ+ N+  V G+ELS +RF + EAAL  L  L P G + I  H PG+ +V+  V
Sbjct:  279 KKTKCSMLYGELLPKGVNRALDAAHLHVKAGSSLVDLGMGTGKVVVQAFLQYSNLNYVLGVELSSSRFRVAEAALLKLKNLHPPGKFEIVCHRPGREIVLREV 381          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig17027.4708.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FY73_ECTSI2.170e-6367.74Topoisomerase 6 subunit B n=1 Tax=Ectocarpus silic... [more]
A0A6H5KB73_9PHAE4.780e-6363.98DOT1 domain-containing protein n=1 Tax=Ectocarpus ... [more]
A0A7S4DH00_HETAK1.080e-2960.91Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A7S3ZW16_9STRA1.740e-2760.61Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
A0A8J2WX67_9STRA7.570e-2760.61Hypothetical protein n=2 Tax=Pelagomonas calceolat... [more]
A0A7S3NGR3_9STRA1.540e-2366.23Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A7S3HRH5_9STRA1.290e-2250.00Hypothetical protein (Fragment) n=1 Tax=Spumella e... [more]
A0A7S1CCX5_9STRA2.200e-2245.54Hypothetical protein n=1 Tax=Bicosoecida sp. CB-20... [more]
A0A5A8CLP2_CAFRO1.990e-2051.02DOT1 domain-containing protein n=3 Tax=Cafeteria r... [more]
A0A7S2SB25_9STRA2.770e-2049.51Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig17027contigL-elsbetiae_contig17027:480..2131 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score219.5
Seed ortholog evalue1.4e-54
Seed eggNOG ortholog2880.D7FY73
Hectar predicted targeting categoryother localisation
EggNOG OGs2C39T@1,2STY5@2759
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons2
Model size597
Cds size597
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815709.22961-CDS-L-elsbetiae_contig17027:479..6981622815709.22961-CDS-L-elsbetiae_contig17027:479..698Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig17027 480..698 +
1691683051.6388962-CDS-L-elsbetiae_contig17027:479..6981691683051.6388962-CDS-L-elsbetiae_contig17027:479..698Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig17027 480..698 +
1622815709.2442725-CDS-L-elsbetiae_contig17027:1753..21311622815709.2442725-CDS-L-elsbetiae_contig17027:1753..2131Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig17027 1754..2131 +
1691683051.6553564-CDS-L-elsbetiae_contig17027:1753..21311691683051.6553564-CDS-L-elsbetiae_contig17027:1753..2131Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig17027 1754..2131 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig17027.4708.1prot_L-elsbetiae_contig17027.4708.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig17027 480..2131 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig17027.4708.1

>prot_L-elsbetiae_contig17027.4708.1 ID=prot_L-elsbetiae_contig17027.4708.1|Name=mRNA_L-elsbetiae_contig17027.4708.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=199bp
KQKKGSLLYGELLPRGVSKAMGPRHLNAAKASTVYDLGMGTGKVAMQVFL
QFPNVKRVYGIELSRARFELGEAALFALMELQPGAYAIKKHIPGQIMVIE
TVAAPKSGSGGGGGGGDGGDATTRDTPQPSLAEGGAGGGAAAAAGKGGLG
GGGAGGGVEGWERICRGAPGRTLEFACGNLFDVENITQASGVDTIYYF*
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mRNA from alignment at L-elsbetiae_contig17027:480..2131+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig17027.4708.1 ID=mRNA_L-elsbetiae_contig17027.4708.1|Name=mRNA_L-elsbetiae_contig17027.4708.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1652bp|location=Sequence derived from alignment at L-elsbetiae_contig17027:480..2131+ (Laminarionema elsbetiae ELsaHSoW15)
AAACAAAAAAAGGGCTCCCTGCTGTACGGCGAGCTCCTCCCCAGGGGCGT CAGCAAGGCCATGGGTCCGAGGCACCTAAACGCGGCCAAGGCGAGCACGG TCTACGACCTCGGGATGGGCACGGGGAAGGTGGCGATGCAGGTGTTCCTG CAGTTCCCGAACGTCAAGAGGGTGTACGGGATCGAGCTGTCGCGGGCTCG CTTCGAGCTCGGAGAGGCGGTGGGTGTTGGTTTCCGTGACAAGTTCCGTC GCTGTTGGCGTTTTTTGGTGTTGTTGGTGTTGTTGGTGTTGTTGTTGCTG TTGGTGTTGTTGTCGTTTGTTGGTGTTGTTGGTAATTGTTAGTGTTGGTG TTGTTGTCGTTTGTTGGTGTTGTCGATGCTGTTGTTGTTGCTACATGCAT GAAGTTTTGATGCTGGTGCTGTTGCTGGTGCGCTGGCGACTATTGTTGGC GTTCTTGGTTTTATTCGCCGTGATTGTTGTAACGGTGGGTGTTGTTGTCA TTCCTATGTTTGAAGTTTTGGTGCTGGTGTGCCGGTGCCGGTGCTGATGC GGATGCTAGTGCTGTCGTCTCGCAGAAACTCCCGCCCGTTATCTCCCTCC AGTACCAGTAACACTCTTGTAGCCACTGCTGGCTCAACGTTGAAGAGACA CGCGGTGTATTCGTCATGCACACAGTAGTAGTAGTTGTTGTCTCCGGCAG TATCTATCGTACATGGATATTTGGATATTTACACGTAGCAAGTACCGCAT ACCGCAGACAGACTGCGCCCGTACGCAAACGCCACCAGTTTTCCACTTTT TGTCGCTCGGGTTGCAACAAGCGTATTTGGGCCTCTGGTGGTGTGACCAC GGTCATGCATGATCGTGCGCACCCCCCCCCCCCCCCCCCCCCCCGCCCGG CCCACTCCACGGTCACGCATGAGATCCCTGTAGCCCCCCCCCCCCCGACG AAACTAATTTATTTCACAGTAGTATGTACCGGCGTACCGGCACGATCATA AGAAACTGAAATTTCGACCGAGGCTGAGTTTCTTAATGCAGTTTGTATAG GATTTAGTGTAGCACTCGCCACGGAGCCCCTTTTTTTGGTAAATCTGAGG CTGGTTTTCGTATGAGTGAGTTTCTTGTGAGCGGGCCGGTAATGTACCCT TTTTCTGTCCCATGTGTCCGCCCATGTACATGAAGGCGGCTGGTTTTCGT ATGAGTGAGTTTCTTGTGAGCGGGCCGGTAATGTACCCTTTTTCTGTCCC ATGTGTCCGCCCATGTATATGAAGGCCCTTTTCGCCTTGATGGAGCTGCA GCCCGGAGCGTACGCCATAAAGAAGCACATCCCGGGGCAGATAATGGTAA TAGAGACCGTCGCCGCACCGAAGAGCGGCAGCGGCGGGGGTGGGGGGGGC GGCGATGGGGGCGACGCCACGACACGCGACACCCCCCAGCCCTCTCTTGC TGAGGGCGGCGCCGGGGGGGGCGCTGCTGCAGCTGCGGGAAAAGGGGGGC TAGGAGGGGGTGGGGCGGGGGGGGGGGTGGAGGGTTGGGAGCGTATCTGC AGGGGCGCGCCGGGGAGAACGCTTGAGTTCGCGTGCGGGAATCTGTTCGA CGTGGAGAACATAACCCAGGCGAGTGGTGTGGATACTATTTACTACTTCT AA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig17027:480..2131+

>mRNA_L-elsbetiae_contig17027.4708.1 ID=mRNA_L-elsbetiae_contig17027.4708.1|Name=mRNA_L-elsbetiae_contig17027.4708.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=1194bp|location=Sequence derived from alignment at L-elsbetiae_contig17027:480..2131+ (Laminarionema elsbetiae ELsaHSoW15)
AAACAAAAAAAGGGCTCCCTGCTGTACGGCGAGCTCCTCCCCAGGGGCGT
CAGCAAGGCCATGGGTCCGAGGCACCTAAACGCGGCCAAGGCGAGCACGG
TCTACGACCTCGGGATGGGCACGGGGAAGGTGGCGATGCAGGTGTTCCTG
CAGTTCCCGAACGTCAAGAGGGTGTACGGGATCGAGCTGTCGCGGGCTCG
CTTCGAGCTCGGAGAGGCGAAACAAAAAAAGGGCTCCCTGCTGTACGGCG
AGCTCCTCCCCAGGGGCGTCAGCAAGGCCATGGGTCCGAGGCACCTAAAC
GCGGCCAAGGCGAGCACGGTCTACGACCTCGGGATGGGCACGGGGAAGGT
GGCGATGCAGGTGTTCCTGCAGTTCCCGAACGTCAAGAGGGTGTACGGGA
TCGAGCTGTCGCGGGCTCGCTTCGAGCTCGGAGAGGCGGCCCTTTTCGCC
TTGATGGAGCTGCAGCCCGGAGCGTACGCCATAAAGAAGCACATCCCGGG
GCAGATAATGGTAATAGAGACCGTCGCCGCACCGAAGAGCGGCAGCGGCG
GGGGTGGGGGGGGCGGCGATGGGGGCGACGCCACGACACGCGACACCCCC
CAGCCCTCTCTTGCTGAGGGCGGCGCCGGGGGGGGCGCTGCTGCAGCTGC
GGGAAAAGGGGGGCTAGGAGGGGGTGGGGCGGGGGGGGGGGTGGAGGGTT
GGGAGCGTATCTGCAGGGGCGCGCCGGGGAGAACGCTTGAGTTCGCGTGC
GGGAATCTGTTCGACGTGGAGAACATAACCCAGGCGAGTGGTGTGGATAC
TATTTACTACTTCTAAGCCCTTTTCGCCTTGATGGAGCTGCAGCCCGGAG
CGTACGCCATAAAGAAGCACATCCCGGGGCAGATAATGGTAATAGAGACC
GTCGCCGCACCGAAGAGCGGCAGCGGCGGGGGTGGGGGGGGCGGCGATGG
GGGCGACGCCACGACACGCGACACCCCCCAGCCCTCTCTTGCTGAGGGCG
GCGCCGGGGGGGGCGCTGCTGCAGCTGCGGGAAAAGGGGGGCTAGGAGGG
GGTGGGGCGGGGGGGGGGGTGGAGGGTTGGGAGCGTATCTGCAGGGGCGC
GCCGGGGAGAACGCTTGAGTTCGCGTGCGGGAATCTGTTCGACGTGGAGA
ACATAACCCAGGCGAGTGGTGTGGATACTATTTACTACTTCTAA
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