mRNA_L-elsbetiae_contig14623.3378.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig14623.3378.1
Unique NamemRNA_L-elsbetiae_contig14623.3378.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig14623.3378.1 vs. uniprot
Match: D8LE29_ECTSI (Thiaminase II n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LE29_ECTSI)

HSP 1 Score: 161 bits (407), Expect = 1.740e-44
Identity = 79/129 (61.24%), Postives = 99/129 (76.74%), Query Frame = 1
Query:    1 LWGDCQLEAMRSLHHPWTLGLATGKTNRASFTNYVAQDAFFLRAFAKAYAYVLIKADDEKSVRAFHSLIGSVLEELTLHSSYAVKWGVDLKN---NPPPVKATTEYVNFLLTVASDPTSSVAQVLAAMV 378
            LW DCQLEAMRSLHHPWTLGLATGKT+ ASF NYVAQDA+FLRAFAKAYAY L KA+DE+ +RAFHSLIGSVLEEL LH+SY+ KWG+D+ N   +  P    + Y  ++ T +S+     A ++ +++
Sbjct:   18 LWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLL 146          
BLAST of mRNA_L-elsbetiae_contig14623.3378.1 vs. uniprot
Match: A0A7S3XK69_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XK69_HETAK)

HSP 1 Score: 154 bits (388), Expect = 3.310e-44
Identity = 75/137 (54.74%), Postives = 91/137 (66.42%), Query Frame = 1
Query:    1 LWGDCQLEAMRSLHHPWTLGLATGKTNRASFTNYVAQDAFFLRAFAKAYAYVLIKADDEKSVRAFHSLIGSVLEELTLHSSYAVKWGVDLKNNPPPVKATTEYVNFLLTVASDPTSSVAQVLAAMVPCMRLYAFLGK 411
            LW D   EA +SLHHPW +GL  G   + SF  Y+AQDAFFL +FAKAYA+ L K  D + + AFH LIG+VL+EL LHS  A +WGVDL     P +AT +Y  FL+  A    S+ A VLAAM PCMRLYA LG+
Sbjct:   53 LWEDNLKEAQKSLHHPWVVGLGLGTLEKESFAQYIAQDAFFLESFAKAYAFALGKCSDREGIVAFHGLIGAVLDELKLHSGLAERWGVDLAALREPKQATDDYTGFLMATAQAADSTPADVLAAMAPCMRLYAHLGR 189          
BLAST of mRNA_L-elsbetiae_contig14623.3378.1 vs. uniprot
Match: A0A6H5J5C1_9PHAE (TENA_THI-4 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J5C1_9PHAE)

HSP 1 Score: 160 bits (404), Expect = 1.700e-43
Identity = 74/92 (80.43%), Postives = 83/92 (90.22%), Query Frame = 1
Query:    1 LWGDCQLEAMRSLHHPWTLGLATGKTNRASFTNYVAQDAFFLRAFAKAYAYVLIKADDEKSVRAFHSLIGSVLEELTLHSSYAVKWGVDLKN 276
            LW DCQLEAMRSLHHPWTLGLATGKT+ ASF NYVAQDA+FLRAFAKAYAY L KA+DE+ +RAFHSLIGSVLEEL LH+SY+ KWG+D+ N
Sbjct:   15 LWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVAN 106          
BLAST of mRNA_L-elsbetiae_contig14623.3378.1 vs. uniprot
Match: A0A835YZC1_9STRA (Putative TenA family transcriptional activator n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YZC1_9STRA)

HSP 1 Score: 142 bits (358), Expect = 1.550e-39
Identity = 74/146 (50.68%), Postives = 96/146 (65.75%), Query Frame = 1
Query:    1 LWGDCQLEAMRSLHHPWTLGLATGKTNRASFTNYVAQDAFFLRA-------FAKAYAYVLIKADDEKSVRAFHSLIGSVLEELTLHSSYAVKWGVDLK---NNPPPVKATTEYVNFLLTVASDPTSSVAQVLAAMVPCMRLYAFLG 408
            LW  C+ EA+ SL+HP+ L LATG   + +F  Y+ QDA +L+A       FA+AY + L KA D + +RAFH L+GS LEELTLH ++A KWGV+L    +   P+ AT  Y +FLL +  D  S+ A VLAAM PCMRLYAFLG
Sbjct:   19 LWRACKEEALNSLYHPFVLQLATGALPKEAFQIYITQDAIYLQARLRRESAFARAYGHALTKAPDAEGMRAFHKLMGSALEELTLHEAFAEKWGVNLAEAIDRDGPLPATRAYTSFLLQIGGDSRSTCADVLAAMAPCMRLYAFLG 164          
BLAST of mRNA_L-elsbetiae_contig14623.3378.1 vs. uniprot
Match: A0A2P6THD7_CHLSO (Family transcriptional regulator n=1 Tax=Chlorella sorokiniana TaxID=3076 RepID=A0A2P6THD7_CHLSO)

HSP 1 Score: 132 bits (331), Expect = 9.030e-36
Identity = 65/140 (46.43%), Postives = 92/140 (65.71%), Query Frame = 1
Query:    1 LWGDCQLEA--MRSLHHPWTLGLATGKTNRASFTNYVAQDAFFLRAFAKAYAYVLIKAD--DEKSVRAFHSLIGSVLEELTLHSSYAVKWGVDLKNNPPPVKATTEYVNFLLTVASDPTSSVAQVLAAMVPCMRLYAFLG 408
            LWGD Q++A  +R+LHHP+ + LA+G   R +F +Y+A+DAFFL++FA+ Y+  + +     E   R    L+  V EEL LH SYA +WGVDL  +  P  AT  YV+++  +A DP   VA +L+AMVPC+RLYAFL 
Sbjct:   15 LWGDAQVQASVLRALHHPFVIALASGVLPREAFQSYIAEDAFFLKSFAEGYSAAISRCGPGQEHVARGLGELLQGVQEELKLHDSYAAEWGVDLSQHQQPNPATRAYVDWVHAIAQDPKQGVAGILSAMVPCLRLYAFLA 154          
BLAST of mRNA_L-elsbetiae_contig14623.3378.1 vs. uniprot
Match: A0A6M8BAV9_9CYAN (TenA family protein n=3 Tax=Thermoleptolyngbya TaxID=2303528 RepID=A0A6M8BAV9_9CYAN)

HSP 1 Score: 128 bits (322), Expect = 1.140e-34
Identity = 67/137 (48.91%), Postives = 88/137 (64.23%), Query Frame = 1
Query:    1 LWGDCQLEAMRSLHHPWTLGLATGKTNRASFTNYVAQDAFFLRAFAKAYAYVLIKADDEKSVRAFHSLIGSVLEELTLHSSYAVKWGVDLKNNPPPVKATTEYVNFLLTVASDPTSSVAQVLAAMVPCMRLYAFLGK 411
            LW + Q  A+  L+HP+  G+  G  ++A F+ YV QDAFFL AFA+AY+    KA D +  + FH+L G VL EL LH SYA++WGVDL++  P   AT  Y +FLL  A   T  V  V  AM+PCM+LYAFLG+
Sbjct:    7 LWQENQDLAIACLNHPFVQGIGDGSLDKAKFSYYVGQDAFFLEAFARAYSIAAAKAPDWQGFQVFHTLAGGVLHELNLHESYAIQWGVDLRSVQPGA-ATRRYTDFLLATAWSQTVGVTAV--AMLPCMKLYAFLGQ 140          
BLAST of mRNA_L-elsbetiae_contig14623.3378.1 vs. uniprot
Match: A0A1G7QCH8_9ACTN (Thiaminase (Transcriptional activator TenA) n=1 Tax=Blastococcus sp. DSM 44268 TaxID=1550231 RepID=A0A1G7QCH8_9ACTN)

HSP 1 Score: 127 bits (320), Expect = 2.400e-34
Identity = 66/137 (48.18%), Postives = 85/137 (62.04%), Query Frame = 1
Query:    1 LWGDCQLEAMRSLHHPWTLGLATGKTNRASFTNYVAQDAFFLRAFAKAYAYVLIKADDEKSVRAFHSLIGSVLEELTLHSSYAVKWGVDLKNNPPPVKATTEYVNFLLTVASDPTSSVAQVLAAMVPCMRLYAFLGK 411
            LW D    A  +L HP+  GLA G   R  FT YVAQDAFFL+AFA+AYA  ++++ D   + AF  L+  V EEL LH  YA +WG+DL     P  AT+ Y +FLL  A+  T  + ++ AAM PCMRLYA LG+
Sbjct:    7 LWADTSDLADATLAHPFVQGLADGSLPRERFTGYVAQDAFFLQAFARAYAMAVVRSPDRAGLEAFADLLAGVREELRLHDGYAARWGIDLAG-VAPAPATSAYTDFLLATAA--TGGLGEICAAMTPCMRLYAHLGQ 140          
BLAST of mRNA_L-elsbetiae_contig14623.3378.1 vs. uniprot
Match: A0A1H8VDR3_9ACTN (Thiaminase (Transcriptional activator TenA) n=1 Tax=Blastococcus endophyticus TaxID=673521 RepID=A0A1H8VDR3_9ACTN)

HSP 1 Score: 125 bits (315), Expect = 1.190e-33
Identity = 66/137 (48.18%), Postives = 85/137 (62.04%), Query Frame = 1
Query:    1 LWGDCQLEAMRSLHHPWTLGLATGKTNRASFTNYVAQDAFFLRAFAKAYAYVLIKADDEKSVRAFHSLIGSVLEELTLHSSYAVKWGVDLKNNPPPVKATTEYVNFLLTVASDPTSSVAQVLAAMVPCMRLYAFLGK 411
            LW D    A  +L HP+  GLA G   R  F  YVAQDAFFL+AFA+ YA  ++++ D   + AF  L+  V +EL LH  YA +WG+DL     PV AT+ Y +FLL  A+  T  + +V AAM PCMRLYAFLG+
Sbjct:    7 LWADSADLADAALAHPFVRGLADGSLPRERFAGYVAQDAFFLQAFARGYAMAVVRSPDRAGLDAFAGLLAGVRDELRLHDGYAARWGIDLAA-VAPVPATSAYTDFLLATAA--TGGLGEVCAAMTPCMRLYAFLGQ 140          
BLAST of mRNA_L-elsbetiae_contig14623.3378.1 vs. uniprot
Match: A0A383WBI9_TETOB (Thiamine phosphate synthase n=2 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383WBI9_TETOB)

HSP 1 Score: 134 bits (336), Expect = 1.290e-33
Identity = 69/140 (49.29%), Postives = 90/140 (64.29%), Query Frame = 1
Query:    1 LWGDCQLEAMRSLHHPWTLGLATGKTNRASFTNYVAQDAFFLRAFAKAYAYVLIKADDEKSVRAFHSLIGSVL----EELTLHSSYAVKWGVDLKNNPPPVKATTEYVNFLLTVASDPTSSVAQVLAAMVPCMRLYAFLG 408
            LW D Q EAM +LHHP+   LA G  +RA F +YV QD FFL  FA+AYA  + K     ++ A  +++  +L     EL LH SYA KWGV +     P  AT++Y +FL+ VA+DP +SVA+VLAAM+PC RLY FLG
Sbjct:   44 LWADSQAEAMEALHHPFVQALAAGTLDRACFQHYVLQDLFFLTRFARAYAAAIDKCGTTPALAAAKAVLAELLLGVDAELKLHGSYAAKWGVAVDEWVWPSAATSQYTDFLMQVATDPETSVAEVLAAMIPCARLYGFLG 183          
BLAST of mRNA_L-elsbetiae_contig14623.3378.1 vs. uniprot
Match: A0A261KY94_9CYAN (TenA family transcriptional regulator n=1 Tax=Hydrocoleum sp. CS-953 TaxID=1671698 RepID=A0A261KY94_9CYAN)

HSP 1 Score: 125 bits (314), Expect = 1.870e-33
Identity = 67/137 (48.91%), Postives = 85/137 (62.04%), Query Frame = 1
Query:    1 LWGDCQLEAMRSLHHPWTLGLATGKTNRASFTNYVAQDAFFLRAFAKAYAYVLIKADDEKSVRAFHSLIGSVLEELTLHSSYAVKWGVDLKNNPPPVKATTEYVNFLLTVASDPTSSVAQVLAAMVPCMRLYAFLGK 411
            LW   Q  A   L HP+  G+A G   + SF  YV QDAFFLRAFA+AY+    K+ + K+   FH+L+G V EEL LH SYA KWGV+L+N  P   AT  Y +FLL  A    +++  +  AM PCMRLYAFLG+
Sbjct:    7 LWKTNQHLATACLSHPFVQGIADGTLPQKSFAYYVGQDAFFLRAFARAYSIAAAKSPEWKTFELFHTLVGGVKEELELHQSYAAKWGVNLENITPGF-ATRRYTDFLLATAWG--NNIGAIATAMTPCMRLYAFLGQ 140          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig14623.3378.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LE29_ECTSI1.740e-4461.24Thiaminase II n=1 Tax=Ectocarpus siliculosus TaxID... [more]
A0A7S3XK69_HETAK3.310e-4454.74Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A6H5J5C1_9PHAE1.700e-4380.43TENA_THI-4 domain-containing protein n=1 Tax=Ectoc... [more]
A0A835YZC1_9STRA1.550e-3950.68Putative TenA family transcriptional activator n=1... [more]
A0A2P6THD7_CHLSO9.030e-3646.43Family transcriptional regulator n=1 Tax=Chlorella... [more]
A0A6M8BAV9_9CYAN1.140e-3448.91TenA family protein n=3 Tax=Thermoleptolyngbya Tax... [more]
A0A1G7QCH8_9ACTN2.400e-3448.18Thiaminase (Transcriptional activator TenA) n=1 Ta... [more]
A0A1H8VDR3_9ACTN1.190e-3348.18Thiaminase (Transcriptional activator TenA) n=1 Ta... [more]
A0A383WBI9_TETOB1.290e-3349.29Thiamine phosphate synthase n=2 Tax=Tetradesmus ob... [more]
A0A261KY94_9CYAN1.870e-3348.91TenA family transcriptional regulator n=1 Tax=Hydr... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig14623contigL-elsbetiae_contig14623:691..1311 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score142.5
Seed ortholog evalue1.4e-31
Seed eggNOG ortholog2880.D8LE29
KEGG rclassRC00224,RC00652,RC02832
KEGG koko:K03707
KEGG ReactionR02133,R09993
KEGG Pathwayko00730,ko01100,map00730,map01100
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0003993,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005829,GO:0006725,GO:0006766,GO:0006767,GO:0006772,GO:0006790,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009110,GO:0009228,GO:0009987,GO:0016311,GO:0016787,GO:0016788,GO:0016791,GO:0017144,GO:0018130,GO:0019438,GO:0034641,GO:0042131,GO:0042364,GO:0042578,GO:0042723,GO:0042724,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044249,GO:0044271,GO:0044272,GO:0044281,GO:0044283,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0071704,GO:0072527,GO:0072528,GO:1901360,GO:1901362,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.TENA/THI-4/PQQC family
EggNOG OGs2QQU0@2759,COG0819@1
EC3.5.99.2
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03000
Ec32 ortholog descriptionThiaminase II
Ec32 orthologEc-05_004270.1
Exons2
Model size420
Cds size393
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig14623.3378.1prot_L-elsbetiae_contig14623.3378.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig14623 718..1311 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815588.2087307-UTR-L-elsbetiae_contig14623:690..7171622815588.2087307-UTR-L-elsbetiae_contig14623:690..717Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig14623 691..717 +
1691682976.3318512-UTR-L-elsbetiae_contig14623:690..7171691682976.3318512-UTR-L-elsbetiae_contig14623:690..717Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig14623 691..717 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815588.224074-CDS-L-elsbetiae_contig14623:717..9661622815588.224074-CDS-L-elsbetiae_contig14623:717..966Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig14623 718..966 +
1691682976.3456404-CDS-L-elsbetiae_contig14623:717..9661691682976.3456404-CDS-L-elsbetiae_contig14623:717..966Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig14623 718..966 +
1622815588.2399442-CDS-L-elsbetiae_contig14623:1167..13111622815588.2399442-CDS-L-elsbetiae_contig14623:1167..1311Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig14623 1168..1311 +
1691682976.3559828-CDS-L-elsbetiae_contig14623:1167..13111691682976.3559828-CDS-L-elsbetiae_contig14623:1167..1311Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig14623 1168..1311 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig14623.3378.1

>prot_L-elsbetiae_contig14623.3378.1 ID=prot_L-elsbetiae_contig14623.3378.1|Name=mRNA_L-elsbetiae_contig14623.3378.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=131bp
MRSLHHPWTLGLATGKTNRASFTNYVAQDAFFLRAFAKAYAYVLIKADDE
KSVRAFHSLIGSVLEELTLHSSYAVKWGVDLKNNPPPVKATTEYVNFLLT
VASDPTSSVAQVLAAMVPCMRLYAFLGKVR*
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mRNA from alignment at L-elsbetiae_contig14623:691..1311+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig14623.3378.1 ID=mRNA_L-elsbetiae_contig14623.3378.1|Name=mRNA_L-elsbetiae_contig14623.3378.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=621bp|location=Sequence derived from alignment at L-elsbetiae_contig14623:691..1311+ (Laminarionema elsbetiae ELsaHSoW15)
TTGTGGGGAGATTGCCAGTTGGAAGCGATGCGATCGTTGCATCACCCGTG GACCCTGGGATTGGCCACCGGAAAAACAAATCGCGCGAGTTTTACGAATT ATGTTGCTCAGGATGCCTTCTTTTTGAGGGCGTTCGCCAAGGCCTACGCC TACGTGCTCATCAAGGCTGATGACGAGAAAAGTGTTCGCGCCTTTCATTC TCTCATCGGATCCGTATTGGAGGAGCTTACCCTGCACTCCTCCTACGCGG TGAAATGGGGGGTTGACCTCAAGAATGTGAGCGAAACAAACAACCTTGTA CCTTCGCGACCCTGATTGTTCATATGCAACCGAAAACTGCATCTCTGTAC TCACTGCTGCCGTGTGTACTTGTTTAAAACATCCGACAATACCCTACTAT ATTGGCTTGAGCATGCCGACAGGTAGCTGTTCTCGATTGGCCCCACTTTA TCTGTCTTGACCCCTCGTGTCAACCAGAACCCTCCACCGGTTAAAGCAAC TACCGAGTACGTGAACTTCCTGTTGACCGTGGCATCGGATCCGACATCGT CAGTTGCCCAAGTTCTGGCCGCGATGGTACCTTGCATGCGGCTGTATGCT TTTCTTGGAAAGGTGCGTTAG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig14623:691..1311+

>mRNA_L-elsbetiae_contig14623.3378.1 ID=mRNA_L-elsbetiae_contig14623.3378.1|Name=mRNA_L-elsbetiae_contig14623.3378.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=786bp|location=Sequence derived from alignment at L-elsbetiae_contig14623:691..1311+ (Laminarionema elsbetiae ELsaHSoW15)
ATGCGATCGTTGCATCACCCGTGGACCCTGGGATTGGCCACCGGAAAAAC
AAATCGCGCGAGTTTTACGAATTATGTTGCTCAGGATGCCTTCTTTTTGA
GGGCGTTCGCCAAGGCCTACGCCTACGTGCTCATCAAGGCTGATGACGAG
AAAAGTGTTCGCGCCTTTCATTCTCTCATCGGATCCGTATTGGAGGAGCT
TACCCTGCACTCCTCCTACGCGGTGAAATGGGGGGTTGACCTCAAGAATA
TGCGATCGTTGCATCACCCGTGGACCCTGGGATTGGCCACCGGAAAAACA
AATCGCGCGAGTTTTACGAATTATGTTGCTCAGGATGCCTTCTTTTTGAG
GGCGTTCGCCAAGGCCTACGCCTACGTGCTCATCAAGGCTGATGACGAGA
AAAGTGTTCGCGCCTTTCATTCTCTCATCGGATCCGTATTGGAGGAGCTT
ACCCTGCACTCCTCCTACGCGGTGAAATGGGGGGTTGACCTCAAGAATAA
CCCTCCACCGGTTAAAGCAACTACCGAGTACGTGAACTTCCTGTTGACCG
TGGCATCGGATCCGACATCGTCAGTTGCCCAAGTTCTGGCCGCGATGGTA
CCTTGCATGCGGCTGTATGCTTTTCTTGGAAAGGTGCGTTAGAACCCTCC
ACCGGTTAAAGCAACTACCGAGTACGTGAACTTCCTGTTGACCGTGGCAT
CGGATCCGACATCGTCAGTTGCCCAAGTTCTGGCCGCGATGGTACCTTGC
ATGCGGCTGTATGCTTTTCTTGGAAAGGTGCGTTAG
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