mRNA_L-elsbetiae_contig13298.2551.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig13298.2551.1
Unique NamemRNA_L-elsbetiae_contig13298.2551.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig13298.2551.1 vs. uniprot
Match: D7G1F0_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G1F0_ECTSI)

HSP 1 Score: 201 bits (511), Expect = 1.870e-61
Identity = 93/94 (98.94%), Postives = 93/94 (98.94%), Query Frame = 1
Query:    1 LYHGQAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFEAKEKKRKER 282
            LYHG AGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFEAKEKKRKER
Sbjct:  174 LYHGMAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFEAKEKKRKER 267          
BLAST of mRNA_L-elsbetiae_contig13298.2551.1 vs. uniprot
Match: A0A7S2SXW6_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2SXW6_9STRA)

HSP 1 Score: 163 bits (412), Expect = 3.800e-48
Identity = 71/92 (77.17%), Postives = 82/92 (89.13%), Query Frame = 1
Query:    1 LYHGQAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFEAKEKKRK 276
            +Y GQAGYK  I+K E+QIGGNKHTGTQGPIRAP F RATCRFDYQPDICKDYK+TGFCGFGDSCKF+HDR +YK+GW +E+E+E K+ KRK
Sbjct:  113 VYRGQAGYKQFIEKKESQIGGNKHTGTQGPIRAPAFFRATCRFDYQPDICKDYKDTGFCGFGDSCKFMHDRGNYKTGWQLEKEWEEKQAKRK 204          
BLAST of mRNA_L-elsbetiae_contig13298.2551.1 vs. uniprot
Match: A0A7S2C6I1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2C6I1_9STRA)

HSP 1 Score: 156 bits (395), Expect = 6.210e-48
Identity = 67/84 (79.76%), Postives = 77/84 (91.67%), Query Frame = 1
Query:    1 LYHGQAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEF 252
            +Y GQAGYKN I+K E+QIGGNK+TGTQGPIRAP F RATCRFDYQPDICKDYK+TGFCGFGDSCKF+HDR ++K+GW MEQE+
Sbjct:   16 VYRGQAGYKNFIEKKESQIGGNKYTGTQGPIRAPQFFRATCRFDYQPDICKDYKDTGFCGFGDSCKFMHDRGNHKTGWQMEQEW 99          
BLAST of mRNA_L-elsbetiae_contig13298.2551.1 vs. uniprot
Match: A0A6V1N7Z0_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1N7Z0_HETAK)

HSP 1 Score: 162 bits (410), Expect = 2.620e-47
Identity = 71/94 (75.53%), Postives = 85/94 (90.43%), Query Frame = 1
Query:    1 LYHGQAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFEAKEKKRKER 282
            +Y GQAGYKN+I+K E+QIGGNK+TGTQGPIRAP+ +RA CRFDYQPDICKDYKETGFCG+GDSCKF+HDR DYKSGW +EQE+E ++K  KE+
Sbjct:   99 IYRGQAGYKNYIEKKESQIGGNKYTGTQGPIRAPSNIRAICRFDYQPDICKDYKETGFCGYGDSCKFIHDRGDYKSGWQLEQEWEEQQKXXKEK 192          
BLAST of mRNA_L-elsbetiae_contig13298.2551.1 vs. uniprot
Match: A0A7S2SX26_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2SX26_9STRA)

HSP 1 Score: 163 bits (412), Expect = 4.270e-46
Identity = 71/92 (77.17%), Postives = 82/92 (89.13%), Query Frame = 1
Query:    1 LYHGQAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFEAKEKKRK 276
            +Y GQAGYK  I+K E+QIGGNKHTGTQGPIRAP F RATCRFDYQPDICKDYK+TGFCGFGDSCKF+HDR +YK+GW +E+E+E K+ KRK
Sbjct:  113 VYRGQAGYKQFIEKKESQIGGNKHTGTQGPIRAPAFFRATCRFDYQPDICKDYKDTGFCGFGDSCKFMHDRGNYKTGWQLEKEWEEKQAKRK 204          
BLAST of mRNA_L-elsbetiae_contig13298.2551.1 vs. uniprot
Match: F0Y2F8_AURAN (Uncharacterized protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y2F8_AURAN)

HSP 1 Score: 155 bits (392), Expect = 1.440e-45
Identity = 65/94 (69.15%), Postives = 85/94 (90.43%), Query Frame = 1
Query:    1 LYHGQAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFEAKEKKRKER 282
            LY GQAGYK+++K +EAQIG NK+TGT+GPIRAP+F+R TCRFDYQPD+CKDYK+TGFCG+GDSCKF+HDR DYK+GW +E E++ ++++ KER
Sbjct:   64 LYQGQAGYKSYVKLNEAQIGANKYTGTKGPIRAPSFVRNTCRFDYQPDVCKDYKDTGFCGYGDSCKFMHDRGDYKTGWQLEAEYQRQKERDKER 157          
BLAST of mRNA_L-elsbetiae_contig13298.2551.1 vs. uniprot
Match: K3WRV5_GLOUD (C3H1-type domain-containing protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WRV5_GLOUD)

HSP 1 Score: 154 bits (389), Expect = 7.680e-45
Identity = 70/91 (76.92%), Postives = 80/91 (87.91%), Query Frame = 1
Query:    1 LYHGQAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFEAKEKKR 273
            +Y GQAGYKN+I K+E+QIG NK+TGTQGPIRA T+ RA  RFDYQPDICKDYKETGFCG+GDSCKFLHDR DYKSGW +E+E+  KEKKR
Sbjct:  126 VYKGQAGYKNYISKNESQIGMNKYTGTQGPIRAQTWARAISRFDYQPDICKDYKETGFCGYGDSCKFLHDRGDYKSGWQIEKEWAEKEKKR 216          
BLAST of mRNA_L-elsbetiae_contig13298.2551.1 vs. uniprot
Match: A0A2D4BFT3_PYTIN (Uncharacterized protein (Fragment) n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BFT3_PYTIN)

HSP 1 Score: 161 bits (408), Expect = 7.840e-45
Identity = 72/94 (76.60%), Postives = 85/94 (90.43%), Query Frame = 1
Query:    1 LYHGQAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFEAKEKKRKER 282
            +Y GQA YKN+IKK+EAQIG NK+TGTQGPIRA T+ R+ CRFDYQPDICKDYKETGFCG+GDSCKFLHDR DYKSGW +E+E+E KE+KR++R
Sbjct:  398 VYRGQAAYKNYIKKNEAQIGMNKYTGTQGPIRAQTWARSICRFDYQPDICKDYKETGFCGYGDSCKFLHDRGDYKSGWQIEREYEEKERKRQQR 491          
BLAST of mRNA_L-elsbetiae_contig13298.2551.1 vs. uniprot
Match: A0A8J2X4S8_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2X4S8_9STRA)

HSP 1 Score: 155 bits (392), Expect = 1.940e-44
Identity = 64/84 (76.19%), Postives = 77/84 (91.67%), Query Frame = 1
Query:    4 YHGQAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFE 255
            Y GQAGYK+++KKDEAQIG NK+TGT+GPIRAP F+R TCRFDYQPD+CKDYK+TGFCG+GDSCKF+HDR DYK+GW +E EF+
Sbjct:  107 YRGQAGYKSYVKKDEAQIGANKYTGTKGPIRAPQFVRNTCRFDYQPDVCKDYKDTGFCGYGDSCKFMHDRGDYKTGWQLEAEFQ 190          
BLAST of mRNA_L-elsbetiae_contig13298.2551.1 vs. uniprot
Match: A0A8K1FPK5_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FPK5_PYTOL)

HSP 1 Score: 153 bits (387), Expect = 7.480e-44
Identity = 69/94 (73.40%), Postives = 81/94 (86.17%), Query Frame = 1
Query:    1 LYHGQAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDICKDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFEAKEKKRKER 282
            +Y GQA YKN++ K E+QIG NK+TGTQGPIRA T+ R+  RFDYQPDICKDYKETGFCG+GDSCKFLHDR DYKSGW +EQE+  KEKKR++R
Sbjct:  127 VYKGQAAYKNYVSKKESQIGMNKYTGTQGPIRAQTWARSISRFDYQPDICKDYKETGFCGYGDSCKFLHDRGDYKSGWQIEQEYAEKEKKRQQR 220          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig13298.2551.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G1F0_ECTSI1.870e-6198.94Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A7S2SXW6_9STRA3.800e-4877.17Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A7S2C6I1_9STRA6.210e-4879.76Hypothetical protein (Fragment) n=1 Tax=Dictyocha ... [more]
A0A6V1N7Z0_HETAK2.620e-4775.53Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A7S2SX26_9STRA4.270e-4677.17Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
F0Y2F8_AURAN1.440e-4569.15Uncharacterized protein (Fragment) n=1 Tax=Aureoco... [more]
K3WRV5_GLOUD7.680e-4576.92C3H1-type domain-containing protein n=1 Tax=Globis... [more]
A0A2D4BFT3_PYTIN7.840e-4576.60Uncharacterized protein (Fragment) n=1 Tax=Pythium... [more]
A0A8J2X4S8_9STRA1.940e-4476.19Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
A0A8K1FPK5_PYTOL7.480e-4473.40Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig13298contigL-elsbetiae_contig13298:3065..4665 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score202.2
Seed ortholog evalue1.1e-49
Seed eggNOG ortholog2880.D7G1F0
Preferred nameRNF113A
KEGG koko:K03661,ko:K13127,ko:K15696
KEGG TC3.A.2.2
KEGG Pathwayko00190,ko01100,ko04142,ko04145,ko04721,ko05110,ko05120,ko05152,ko05323,map00190,map01100,map04142,map04145,map04721,map05110,map05120,map05152,map05323
KEGG ModuleM00160
Hectar predicted targeting categoryother localisation
GOsGO:0000375,GO:0000377,GO:0000398,GO:0003674,GO:0003824,GO:0004842,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005681,GO:0005684,GO:0006139,GO:0006396,GO:0006397,GO:0006464,GO:0006725,GO:0006807,GO:0007275,GO:0007399,GO:0008150,GO:0008152,GO:0008270,GO:0008380,GO:0009987,GO:0010467,GO:0010468,GO:0016020,GO:0016070,GO:0016071,GO:0016074,GO:0016567,GO:0016740,GO:0019219,GO:0019222,GO:0019538,GO:0019787,GO:0022008,GO:0030154,GO:0030182,GO:0031323,GO:0032446,GO:0032501,GO:0032502,GO:0032991,GO:0034247,GO:0034470,GO:0034641,GO:0034660,GO:0036211,GO:0043144,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043484,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0045292,GO:0046483,GO:0046872,GO:0046914,GO:0048699,GO:0048731,GO:0048856,GO:0048869,GO:0050789,GO:0050794,GO:0051171,GO:0051252,GO:0060255,GO:0065007,GO:0070647,GO:0071704,GO:0080090,GO:0090304,GO:0140096,GO:1901360,GO:1901564,GO:1990904
EggNOG free text desc.snoRNA splicing
EggNOG OGsCOG5152@1,KOG1813@2759
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03041,ko04121
Ec32 ortholog descriptionZinc finger, CCCH-type
Ec32 orthologEc-07_000790.1
Exons3
Model size282
Cds size282
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig13298.2551.1prot_L-elsbetiae_contig13298.2551.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig13298 3065..4665 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815505.106609-CDS-L-elsbetiae_contig13298:3064..31181622815505.106609-CDS-L-elsbetiae_contig13298:3064..3118Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13298 3065..3118 +
1691682930.95469-CDS-L-elsbetiae_contig13298:3064..31181691682930.95469-CDS-L-elsbetiae_contig13298:3064..3118Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13298 3065..3118 +
1622815505.120005-CDS-L-elsbetiae_contig13298:3616..37271622815505.120005-CDS-L-elsbetiae_contig13298:3616..3727Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13298 3617..3727 +
1691682930.9697716-CDS-L-elsbetiae_contig13298:3616..37271691682930.9697716-CDS-L-elsbetiae_contig13298:3616..3727Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13298 3617..3727 +
1622815505.1315641-CDS-L-elsbetiae_contig13298:4548..46651622815505.1315641-CDS-L-elsbetiae_contig13298:4548..4665Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13298 4549..4665 +
1691682930.979821-CDS-L-elsbetiae_contig13298:4548..46651691682930.979821-CDS-L-elsbetiae_contig13298:4548..4665Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13298 4549..4665 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig13298.2551.1

>prot_L-elsbetiae_contig13298.2551.1 ID=prot_L-elsbetiae_contig13298.2551.1|Name=mRNA_L-elsbetiae_contig13298.2551.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=94bp
LYHGQAGYKNHIKKDEAQIGGNKHTGTQGPIRAPTFLRATCRFDYQPDIC
KDYKETGFCGFGDSCKFLHDRADYKSGWAMEQEFEAKEKKRKER
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mRNA from alignment at L-elsbetiae_contig13298:3065..4665+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig13298.2551.1 ID=mRNA_L-elsbetiae_contig13298.2551.1|Name=mRNA_L-elsbetiae_contig13298.2551.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1601bp|location=Sequence derived from alignment at L-elsbetiae_contig13298:3065..4665+ (Laminarionema elsbetiae ELsaHSoW15)
CTTTACCACGGACAGGCAGGATACAAGAACCACATCAAGAAAGATGAGGC GCAGGTAAAAAAACGAGATTCATGGGGTTGCGTGCAACAGCCTTTAAAAT ACGTACATACCACATGTAGTAGAACACACGAAGAGAGCGCACGAAATGTC GGGATGCTGCTGGTGAGCAGGAAAAGACACGGCGGAGACTTTACGGCCCT GCCTCACCAGGCGCTGCTGCTGCTGCTGCACAGTTTCGGTGCCCCCCCCC CCTCCGACAACTTTAGATTGGAAGGTTTTCCAGGCCGACGAGATCAGCAT TTCTCCGCTGAAGGGGGATTAGTACACTACGCGACTGCTGTCTGTTATGG CGTGTGCCAGTTTCAGCTGGTTTTGATGCAGTCGGCCGGTGCGGTCCTCT GGTTCTCTCTGCGCATGTGCCTCAGCTGGAAGGGTCTCTTTAACCGCACG CGCTTCCAACACATGTATGTGACCTGATTTCCTGCACGTGCCCGCCGAAC GAACGACCTGGCTGTTCATGACACCTCTGCCAACCGTGCTCTCGTGCTGT AGATCGGGGGCAACAAGCACACGGGGACCCAGGGCCCGATCAGAGCGCCG ACCTTCCTGCGGGCCACCTGCCGGTTCGACTACCAGCCAGATATCTGCAA GGACTACAAAGAGGTACGGTACGGTACGGTACGGTGCAGTACGGTACGGT ACGGTACGGGGTGCGGTGCGGTGCGGTGCGGTGCGGTACGGTGCGTTGCG TTGCGGTGCGGTACGGCACGGTACGGCACGGTATGTACGGTAACGGCCAC GGTAACGGTACTGTAGTATGTTGGAGAGCGTGTGGCGCCGTCGGTGCTCC TTGTTTCCCGTCGCCTCGCGCTGGGACGACCTTTTTGTTGTCCATTCGCT GTATTCGTGCACTGATGCCTTGGGTACTACTGTCTGTCGATCGCTAGAGC ACTAAGTTCTCCGTCCGCTGGCCACACCGCGCGGCGATTGGTCCCCCGAA TATTCACAACATACATAGACGGGAGCCCTCACCCCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCC TGTCGGGGTTTTTTTNNNNNNNNNNCCTTATTTTTCCCCCCCGCGGCCCA CCCCGCCCGGCTGTGGGCCCCCCAAAAATTCCAAACCTACCACAACGGGG CCCCCCCCCCCCCCCCCCCCCCTCCTCCTTTCCTCGCAAGCACCGAACCC AGAAGTGCCCGTGTACACGTGTGTTTGCGTATCTTTCTGCTCTCTCTCTC TGTGGGTGTGGGTTGCTGTTTTCCGGCCGTTTTTTGTGCCTGCGACTCTC GGCACTGGATACCGCCATTTGGTCTTTCTACGTGTACACCGCGCTCGAGT TTGCATGCCTTTTTCCGCGTCTCAACATTTTTTTGTTTTGCCTGTTTTTT TATTTTATTTGCTTGTGTCGTTTCGTTTTTTCAGACCGGCTTTTGCGGTT TCGGAGATAGCTGCAAGTTCCTGCACGACCGGGCGGATTACAAGTCGGGG TGGGCCATGGAGCAGGAGTTCGAGGCGAAGGAGAAGAAGAGGAAGGAGCG G
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Coding sequence (CDS) from alignment at L-elsbetiae_contig13298:3065..4665+

>mRNA_L-elsbetiae_contig13298.2551.1 ID=mRNA_L-elsbetiae_contig13298.2551.1|Name=mRNA_L-elsbetiae_contig13298.2551.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=564bp|location=Sequence derived from alignment at L-elsbetiae_contig13298:3065..4665+ (Laminarionema elsbetiae ELsaHSoW15)
CTTTACCACGGACAGGCAGGATACAAGAACCACATCAAGAAAGATGAGGC
GCAGCTTTACCACGGACAGGCAGGATACAAGAACCACATCAAGAAAGATG
AGGCGCAGATCGGGGGCAACAAGCACACGGGGACCCAGGGCCCGATCAGA
GCGCCGACCTTCCTGCGGGCCACCTGCCGGTTCGACTACCAGCCAGATAT
CTGCAAGGACTACAAAGAGATCGGGGGCAACAAGCACACGGGGACCCAGG
GCCCGATCAGAGCGCCGACCTTCCTGCGGGCCACCTGCCGGTTCGACTAC
CAGCCAGATATCTGCAAGGACTACAAAGAGACCGGCTTTTGCGGTTTCGG
AGATAGCTGCAAGTTCCTGCACGACCGGGCGGATTACAAGTCGGGGTGGG
CCATGGAGCAGGAGTTCGAGGCGAAGGAGAAGAAGAGGAAGGAGCGGACC
GGCTTTTGCGGTTTCGGAGATAGCTGCAAGTTCCTGCACGACCGGGCGGA
TTACAAGTCGGGGTGGGCCATGGAGCAGGAGTTCGAGGCGAAGGAGAAGA
AGAGGAAGGAGCGG
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