mRNA_L-elsbetiae_contig11817.1569.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig11817.1569.1
Unique NamemRNA_L-elsbetiae_contig11817.1569.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
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BLAST of mRNA_L-elsbetiae_contig11817.1569.1 vs. uniprot
Match: A0A6H5L4Q1_9PHAE (Cysteine-rich DPF motif domain-containing protein 1 n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L4Q1_9PHAE)

HSP 1 Score: 137 bits (345), Expect = 6.580e-36
Identity = 63/84 (75.00%), Postives = 70/84 (83.33%), Query Frame = 2 
Query:  488 MEDVFCMKDPFSIGG---GDAGGDRKCLPTVVGGTCACCSNDVCAQCSLFYSVRLCGPCAREHRLRLPSELHRMVDATGGPPTK 730
            MEDV+CM+DPFS+GG   G  G +RKCLPTVVGGTCA CS DVCAQCSLFY+VRLCG CAR H+  LPSELHR+VDATGG P K
Sbjct:    1 MEDVYCMRDPFSVGGAGGGATGENRKCLPTVVGGTCAVCSKDVCAQCSLFYAVRLCGHCARAHKASLPSELHRIVDATGGAPEK 84
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BLAST of mRNA_L-elsbetiae_contig11817.1569.1 vs. uniprot
Match: A0A7S1EEK0_HEMAN (Cysteine-rich DPF motif domain-containing protein 1 n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1EEK0_HEMAN)

HSP 1 Score: 90.1 bits (222), Expect = 2.560e-18
Identity = 45/107 (42.06%), Postives = 58/107 (54.21%), Query Frame = 2 
Query:  407 FTCSLCGGCFRVDYHGRKPPFCPQLVFMEDVFCMKDPFSIGGGDAGGDRKCLPTVVGGTCACCSNDVCAQCSLFYSVRLCGPCAREHRLRLPSELHRMVDAT-GGPP 724
            FTC +C       Y GR P   PQ+V +ED F   DPF+          +  P  +GG CA CS  VC  CSL+Y+ R+CG CARE R  +P E+H ++D   GG P
Sbjct:   13 FTCYVCNFTMPYVYFGRTPKMAPQMVLLEDSFVANDPFT---------PEARPLCLGGKCAICSACVCVTCSLYYTKRVCGKCAREVRGAMPVEMHAVIDRLPGGAP 110
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BLAST of mRNA_L-elsbetiae_contig11817.1569.1 vs. uniprot
Match: UPI001144DCA7 (cysteine-rich DPF motif domain-containing protein 1 n=1 Tax=Formica exsecta TaxID=72781 RepID=UPI001144DCA7)

HSP 1 Score: 86.3 bits (212), Expect = 1.030e-16
Identity = 41/101 (40.59%), Postives = 58/101 (57.43%), Query Frame = 2 
Query:  404 SFTCSLCGGCFRVDYHGRKPPFCPQLVFMEDVFCMKDPFSIGGGDAGGDRKCLPTVVGGTCACCSNDVCAQCSLFYSVRLCGPCAREHRLRLPSELHRMVD 706
            +FTCS C    R DY G KPPF  QLV++E+ + MKDPFS+         +    V+G  C+ C  +VC  CS+FY+ R C  CA  +  +LP +LH  ++
Sbjct:   32 TFTCSFCYLKERYDYKGAKPPFARQLVYLEECYIMKDPFSL-------PNRGEVLVLGADCSMCKRNVCLGCSIFYTQRFCLKCASNNMQKLPLQLHGKIN 125
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BLAST of mRNA_L-elsbetiae_contig11817.1569.1 vs. uniprot
Match: E2B997_HARSA (Cysteine-rich DPF motif domain-containing protein 1 n=1 Tax=Harpegnathos saltator TaxID=610380 RepID=E2B997_HARSA)

HSP 1 Score: 85.1 bits (209), Expect = 1.410e-16
Identity = 40/104 (38.46%), Postives = 58/104 (55.77%), Query Frame = 2 
Query:  392 KSRSSFTCSLCGGCFRVDYHGRKPPFCPQLVFMEDVFCMKDPFSIGGGDAGGDRKCLPTVVGGTCACCSNDVCAQCSLFYSVRLCGPCAREHRLRLPSELHRMV 703
            +S  +F CS C      DY G +PPF  QLV++E+ + MKDPFS+         +    V+GG C+ C + VC  CS+FY+ R C  CA  +   LP++LH  +
Sbjct:    4 RSGGTFACSFCSLKEHYDYKGTRPPFARQLVYLEECYVMKDPFSL-------PSRGEVLVLGGDCSTCGHAVCLGCSIFYTKRFCPKCASSNMHNLPNKLHGKI 100
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BLAST of mRNA_L-elsbetiae_contig11817.1569.1 vs. uniprot
Match: UPI001069289D (cysteine-rich DPF motif domain-containing protein 1-like n=1 Tax=Dendronephthya gigantea TaxID=151771 RepID=UPI001069289D)

HSP 1 Score: 83.6 bits (205), Expect = 4.930e-16
Identity = 40/113 (35.40%), Postives = 63/113 (55.75%), Query Frame = 2 
Query:  386 DGKSRSS-FTCSLCGGCFRVDYHGRKPPFCPQLVFMEDVFCMKDPFSIGGGDAGGDRKCLPTVVGGTCACCSNDVCA--QCSLFYSVRLCGPCAREHRLRLPSELHRMVDATG 715
            +GK+ S  F C  C      DY G++PPFC +++ +E V+ +KDPF+         R+    ++GG CA C  +VC   +CS+FYS R C PC+ ++    P+E+ + V   G
Sbjct:    2 EGKAHSMVFQCKNCNFSTHYDYFGKQPPFCKEIILLESVYVIKDPFT---------RETGIVILGGHCALCHTEVCCDQKCSVFYSKRFCIPCSMKYFKEFPAEIQKDVQKNG 105
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BLAST of mRNA_L-elsbetiae_contig11817.1569.1 vs. uniprot
Match: UPI00143CC227 (cysteine-rich DPF motif domain-containing protein 1 n=1 Tax=Belonocnema kinseyi TaxID=2817044 RepID=UPI00143CC227)

HSP 1 Score: 84.3 bits (207), Expect = 6.420e-16
Identity = 41/105 (39.05%), Postives = 57/105 (54.29%), Query Frame = 2 
Query:  392 KSRSSFTCSLCGGCFRVDYHGRKPPFCPQLVFMEDVFCMKDPFS-IGGGDAGGDRKCLPTVVGGTCACCSNDVCAQCSLFYSVRLCGPCAREHRLRLPSELHRMV 703
            ++R +F C  C      DY G KPPF  QL+++ED + MKDPFS +  G+          V+G  C+ C   VC  CS+FY+ R C  CA  H   LP +LH+ +
Sbjct:   36 ETRGTFACGFCPLNEHFDYKGTKPPFARQLLYLEDCYIMKDPFSPLNQGET--------LVLGADCSFCKTAVCLGCSIFYTKRFCKKCALAHIKDLPKQLHKKI 132
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BLAST of mRNA_L-elsbetiae_contig11817.1569.1 vs. uniprot
Match: UPI00063F3381 (cysteine-rich DPF motif domain-containing protein 1 n=1 Tax=Monomorium pharaonis TaxID=307658 RepID=UPI00063F3381)

HSP 1 Score: 83.2 bits (204), Expect = 1.160e-15
Identity = 40/100 (40.00%), Postives = 55/100 (55.00%), Query Frame = 2 
Query:  404 SFTCSLCGGCFRVDYHGRKPPFCPQLVFMEDVFCMKDPFSIGGGDAGGDRKCLPTVVGGTCACCSNDVCAQCSLFYSVRLCGPCAREHRLRLPSELHRMV 703
            +FTCS C      DY G KPPF  QLV++E+ + MKDPFS+         +    V+G  C+ C + VC  CS+FY+ R C  CA  +   LP +LH  +
Sbjct:   28 TFTCSFCPLKEHYDYKGAKPPFARQLVYLEECYIMKDPFSL-------PNRGEVLVLGADCSMCGHAVCLGCSIFYTKRFCPKCASSNMQYLPPQLHGKI 120
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BLAST of mRNA_L-elsbetiae_contig11817.1569.1 vs. uniprot
Match: UPI0006B0E3AA (cysteine-rich DPF motif domain-containing protein 1-like isoform X1 n=2 Tax=Limulus polyphemus TaxID=6850 RepID=UPI0006B0E3AA)

HSP 1 Score: 82.0 bits (201), Expect = 2.130e-15
Identity = 38/101 (37.62%), Postives = 60/101 (59.41%), Query Frame = 2 
Query:  407 FTCSLCGGCFRVDYHGRKPPFCPQLVFMEDVFCMKDPFSIGGGDAGGDRKCLPTVVGGTCACCSNDVCA--QCSLFYSVRLCGPCAREHRLRLPSELHRMV 703
            FTC +CG      Y G++PPF   ++F+EDV+  KDPFS  G       + +  ++GG C+ C N VC   +CS+++S R C  CA+++ L+ P EL + +
Sbjct:   18 FTCYMCGMTETFHYFGKRPPFLKSILFLEDVYLAKDPFSPAG-------EGIFLLLGGHCSLCKNIVCQAEECSIYFSKRFCLSCAKDNSLQFPPELRKAI 111
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BLAST of mRNA_L-elsbetiae_contig11817.1569.1 vs. uniprot
Match: UPI0005F47033 (cysteine-rich DPF motif domain-containing protein 1-like n=2 Tax=Vollenhovia emeryi TaxID=411798 RepID=UPI0005F47033)

HSP 1 Score: 82.4 bits (202), Expect = 2.250e-15
Identity = 41/100 (41.00%), Postives = 54/100 (54.00%), Query Frame = 2 
Query:  404 SFTCSLCGGCFRVDYHGRKPPFCPQLVFMEDVFCMKDPFSIGGGDAGGDRKCLPTVVGGTCACCSNDVCAQCSLFYSVRLCGPCAREHRLRLPSELHRMV 703
            +FTCS C    R DY G +PPF  QLV+ E+ + MKDPFS+         K    V+G  C+ C + VC  CS+FY  R C  CA  +   LP +LH  +
Sbjct:   29 TFTCSFCPLKERYDYKGTRPPFARQLVYSEECYVMKDPFSL-------PNKGEILVLGADCSVCEHAVCLGCSIFYKRRFCPKCASSNIQYLPLQLHNKI 121
[Close]
BLAST of mRNA_L-elsbetiae_contig11817.1569.1 vs. uniprot
Match: A0A0D2VYM5_CAPO3 (Cysteine-rich DPF motif domain-containing protein 1 n=1 Tax=Capsaspora owczarzaki (strain ATCC 30864) TaxID=595528 RepID=A0A0D2VYM5_CAPO3)

HSP 1 Score: 82.8 bits (203), Expect = 3.660e-15
Identity = 44/103 (42.72%), Postives = 57/103 (55.34%), Query Frame = 2 
Query:  398 RSSFTCSLCGGCFRVDYHGRKPPFCPQLVFMEDVFCMKDPFSIGGGDAGGDRKCLPT---VVGGTCACCSNDVCAQ--CSLFYSVRLCGPCAREHRLRLPSEL 691
            R S  CSLC      DY G+ PPF P++VF+EDVF ++DPF++    A      LP     +G  CA C+  VC Q  CSLFY  R C  CA++     PS+L
Sbjct:   35 RLSTQCSLCAHPVPYDYTGKTPPFAPRVVFLEDVFAIRDPFAVAAQTASLAAASLPKRCLAMGARCAECNRIVCMQATCSLFYQKRFCALCAKQWANCFPSQL 137
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig11817.1569.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5L4Q1_9PHAE6.580e-3675.00Cysteine-rich DPF motif domain-containing protein ... [more]
A0A7S1EEK0_HEMAN2.560e-1842.06Cysteine-rich DPF motif domain-containing protein ... [more]
UPI001144DCA71.030e-1640.59cysteine-rich DPF motif domain-containing protein ... [more]
E2B997_HARSA1.410e-1638.46Cysteine-rich DPF motif domain-containing protein ... [more]
UPI001069289D4.930e-1635.40cysteine-rich DPF motif domain-containing protein ... [more]
UPI00143CC2276.420e-1639.05cysteine-rich DPF motif domain-containing protein ... [more]
UPI00063F33811.160e-1540.00cysteine-rich DPF motif domain-containing protein ... [more]
UPI0006B0E3AA2.130e-1537.62cysteine-rich DPF motif domain-containing protein ... [more]
UPI0005F470332.250e-1541.00cysteine-rich DPF motif domain-containing protein ... [more]
A0A0D2VYM5_CAPO33.660e-1542.72Cysteine-rich DPF motif domain-containing protein ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig11817contigL-elsbetiae_contig11817:49..1944 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score81.3
Seed ortholog evalue4e-13
Seed eggNOG ortholog192875.XP_004343823.1
Preferred nameCDPF1
KEGG koko:K22073
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Cysteine-rich domain
EggNOG OGsKOG4543@1,KOG4543@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03029
Ec32 ortholog descriptionHypothetical protein
Ec32 orthologEc-08_006030.1
Exons2
Model size1235
Cds size417
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig11817.1569.1prot_L-elsbetiae_contig11817.1569.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig11817 371..1448 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815385.0447447-UTR-L-elsbetiae_contig11817:48..3701622815385.0447447-UTR-L-elsbetiae_contig11817:48..370Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig11817 49..370 +
1691682872.1523037-UTR-L-elsbetiae_contig11817:48..3701691682872.1523037-UTR-L-elsbetiae_contig11817:48..370Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig11817 49..370 +
1622815385.0794482-UTR-L-elsbetiae_contig11817:1448..19441622815385.0794482-UTR-L-elsbetiae_contig11817:1448..1944Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig11817 1449..1944 +
1691682872.19536-UTR-L-elsbetiae_contig11817:1448..19441691682872.19536-UTR-L-elsbetiae_contig11817:1448..1944Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig11817 1449..1944 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815385.05439-CDS-L-elsbetiae_contig11817:370..5981622815385.05439-CDS-L-elsbetiae_contig11817:370..598Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig11817 371..598 +
1691682872.1646764-CDS-L-elsbetiae_contig11817:370..5981691682872.1646764-CDS-L-elsbetiae_contig11817:370..598Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig11817 371..598 +
1622815385.0674329-CDS-L-elsbetiae_contig11817:1259..14481622815385.0674329-CDS-L-elsbetiae_contig11817:1259..1448Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig11817 1260..1448 +
1691682872.184753-CDS-L-elsbetiae_contig11817:1259..14481691682872.184753-CDS-L-elsbetiae_contig11817:1259..1448Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig11817 1260..1448 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig11817.1569.1

>prot_L-elsbetiae_contig11817.1569.1 ID=prot_L-elsbetiae_contig11817.1569.1|Name=mRNA_L-elsbetiae_contig11817.1569.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=139bp
MAHSGRGPNHIAASGPPPIACDGKSRSSFTCSLCGGCFRVDYHGRKPPFC
PQLVFMEDVFCMKDPFSIGGGDAGGDRKCLPTVVGGTCACCSNDVCAQCS
LFYSVRLCGPCAREHRLRLPSELHRMVDATGGPPTKDM*
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mRNA from alignment at L-elsbetiae_contig11817:49..1944+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig11817.1569.1 ID=mRNA_L-elsbetiae_contig11817.1569.1|Name=mRNA_L-elsbetiae_contig11817.1569.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1896bp|location=Sequence derived from alignment at L-elsbetiae_contig11817:49..1944+ (Laminarionema elsbetiae ELsaHSoW15)
TATTATTGCTTTGCTGGCCCTTCGGGTCCATTTTATTTTTCGTGTACCTT
TTCTTAGGTATAAGTTAGCCTGTGACCACGGGAATTGACCGCATCAAGTG
TTGGGTTAGTACGGACATTGAGAGGTACTGGATGGAGAGATGGGAACGAT
TTTTAATGTGAAGACAACATACAACAACACAACATATCACCAAGAGCAAG
CATAAATTATCACACGACAAATCCACACTTAGTCTAGTTCTTCAAGCCCG
CCCCCCCCTGCCGCCGCCGCCCCTCGCCAATGTCTAAGCTGTCAGCCCGG
GGTGGGTATTCGAAGCGCGGGCATGGCACATTCTGGGCGTGGACCGAACC
ATATCGCAGCCAGCGGGCCACCGCCGATAGCGTGCGACGGCAAGTCGAGG
TCATCCTTCACGTGCTCGTTGTGCGGGGGGTGTTTTCGCGTCGACTACCA
CGGCAGAAAACCACCGTTCTGTCCCCAGCTAGTCTTCATGGAAGACGTAT
TTTGCATGAAGGACCCCTTTTCTATAGGCGGGGGAGACGCAGGGGGGGAT
GTGAGTGTCGCCCACGGATTCGGCTTGAAGCTTTCATAAAGCTGGGCCAA
CTACCTTTGAGCTTTGCTGTCGCTTGAAGACATCATCATCTGGTCTGATG
TATAGCGCGTAGAAGCCTAGACTACCTGAAGCGAAGAAAACCCAACTGCT
CGCCAATTACTCGCCGCATGATTTCCTCCAAGGCACGGTGCCTGTGGTAC
CTTGTTGAAAGGAAGCACCGGAGGTTCCCCTCGAGTATGTCTCTGTGGAA
TACAACCACCCTGTGGCCCGCTGCACATTTCGCCCCCGGTACATGTTTTA
CGCCACACAGTAGTATTAGCTGAGTGTTGCAACCGCCAAAAGGTCGGGTT
CCTAAATAAGATGTACACGCTTAGGACTGCGAAAATAGTTGTGATCCGGC
GTTCAATGCTTATTCTTTTGAGCTGCACCGCGCCTTTTGATGTTTGTTTT
TCCTCGCGCCTGCGCACTATGACTCCGTTGTCGTGCATGTTGGGTTTTCG
AAGAGTATGCATTTCCGTTTCGTTACTCCTCGCAACCTCCTGCATCCGCC
CCACATCCCCAATTTCTCCCTTCGACCGCGGCTGACGCAATATCATCATC
TGGTTGAGCGTCTGGTCATTCATGTCCCCTGCTGCTGTCGCGTGTCTTTC
CATCCTCTCAGAGAAAATGTTTACCAACGGTGGTCGGCGGAACTTGTGCT
TGCTGTTCCAATGACGTCTGCGCCCAGTGCAGCTTGTTCTACTCCGTGAG
GCTCTGCGGCCCGTGCGCGCGGGAGCACAGGTTACGGTTGCCCTCAGAGT
TGCACCGTATGGTGGATGCCACCGGGGGACCACCGACGAAGGACATGTAG
TTGATAGAGCGAGAAGATACACTATCAAAAATGTGACGGGAATACTGTAG
CAGAGATGAAAATGGTAGCATTTGGTAGATACATAGCTGCGGCTGACGAC
CCAAGGGTGGCGAACGACATGAACAATGCACCGACGGGTTAAGTGGTTTG
TGCGTTCTGGTCCCGACGGGGTTTGTTTGTCATTGTGCGTATTTGGTCAT
GTCAGGCAAGGGGTTGCGGTGGGGGCTGTGCAGGGTGGGACTCGCATGCA
AATCTGTTATGTGCATGCATTCATTGTGTACATTTTCTAGCTGAACTGAA
GTGACGCGAGCTTCACGAGCTAGATTTGGTGGCGGCGGTCTGTTTTCACT
GCAAGGTGAACACCCCGCAGCAGTAGGAGGCTCTCCTCTTGAGTGTGGCG
GCTCTGCCTCTCTTGTATGTAGACTATAGTCTATATGTACATTGCAGGCG
CGAGAATAATAATCATGTTGACTAGATTTTGACAAATTAACCCTAG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig11817:49..1944+

>mRNA_L-elsbetiae_contig11817.1569.1 ID=mRNA_L-elsbetiae_contig11817.1569.1|Name=mRNA_L-elsbetiae_contig11817.1569.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=834bp|location=Sequence derived from alignment at L-elsbetiae_contig11817:49..1944+ (Laminarionema elsbetiae ELsaHSoW15)
ATGGCACATTCTGGGCGTGGACCGAACCATATCGCAGCCAGCGGGCCACC
GCCGATAGCGTGCGACGGCAAGTCGAGGTCATCCTTCACGTGCTCGTTGT
GCGGGGGGTGTTTTCGCGTCGACTACCACGGCAGAAAACCACCGTTCTGT
CCCCAGCTAGTCTTCATGGAAGACGTATTTTGCATGAAGGACCCCTTTTC
TATAGGCGGGGGAGACGCAGGGGGGGATATGGCACATTCTGGGCGTGGAC
CGAACCATATCGCAGCCAGCGGGCCACCGCCGATAGCGTGCGACGGCAAG
TCGAGGTCATCCTTCACGTGCTCGTTGTGCGGGGGGTGTTTTCGCGTCGA
CTACCACGGCAGAAAACCACCGTTCTGTCCCCAGCTAGTCTTCATGGAAG
ACGTATTTTGCATGAAGGACCCCTTTTCTATAGGCGGGGGAGACGCAGGG
GGGGATAGAAAATGTTTACCAACGGTGGTCGGCGGAACTTGTGCTTGCTG
TTCCAATGACGTCTGCGCCCAGTGCAGCTTGTTCTACTCCGTGAGGCTCT
GCGGCCCGTGCGCGCGGGAGCACAGGTTACGGTTGCCCTCAGAGTTGCAC
CGTATGGTGGATGCCACCGGGGGACCACCGACGAAGGACATGTAGAGAAA
ATGTTTACCAACGGTGGTCGGCGGAACTTGTGCTTGCTGTTCCAATGACG
TCTGCGCCCAGTGCAGCTTGTTCTACTCCGTGAGGCTCTGCGGCCCGTGC
GCGCGGGAGCACAGGTTACGGTTGCCCTCAGAGTTGCACCGTATGGTGGA
TGCCACCGGGGGACCACCGACGAAGGACATGTAG
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