mRNA_L-elsbetiae_contig5954.14445.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig5954.14445.1
Unique NamemRNA_L-elsbetiae_contig5954.14445.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
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BLAST of mRNA_L-elsbetiae_contig5954.14445.1 vs. uniprot
Match: A0A6H5L1F8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L1F8_9PHAE)

HSP 1 Score: 114 bits (286), Expect = 2.890e-26
Identity = 104/174 (59.77%), Postives = 114/174 (65.52%), Query Frame = 1 
Query:    1 VNLSERKAFLKEKVTAIMLEGEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTKRTDFQLS---PALYEFMGSKETMMARGD----VTKAVWAYIRAASLQDPKDRRKIRNDDKMFRVFKCKTMNMMHIAKKLSPHLKKYSDLVVG-------DTESCSS 480
            V+LSE+KAFLKE VTAIM EG+ XXXXXXXXXXXXXXXXXXXXXX                     ++R   + S   PA    +    +  AR      VTKAVWAYIRA SLQDPKD RKIRND+KMFRVFKCKTMNMMHIAKKLSPHLKK SDLV         DTE+ SS
Sbjct:   41 VDLSEKKAFLKETVTAIMSEGDEXXXXXXXXXXXXXXXXXXXXXX---------------------SQRARLRPSASPPASSSPLPCTSSWAARTRAWPAVTKAVWAYIRAESLQDPKDGRKIRNDEKMFRVFKCKTMNMMHIAKKLSPHLKKVSDLVESGAPNEEVDTEASSS 193
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BLAST of mRNA_L-elsbetiae_contig5954.14445.1 vs. uniprot
Match: D7G2V1_ECTSI (SWIB/MDM2 domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G2V1_ECTSI)

HSP 1 Score: 111 bits (277), Expect = 1.030e-25
Identity = 55/58 (94.83%), Postives = 55/58 (94.83%), Query Frame = 1 
Query:  280 VTKAVWAYIRAASLQDPKDRRKIRNDDKMFRVFKCKTMNMMHIAKKLSPHLKKYSDLV 453
            VTKAVWAYIRA SLQDPKDRRKIRNDDKMF VFKCKTMNMMHIAKKLSPHLKK SDLV
Sbjct:   56 VTKAVWAYIRAESLQDPKDRRKIRNDDKMFPVFKCKTMNMMHIAKKLSPHLKKLSDLV 113
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BLAST of mRNA_L-elsbetiae_contig5954.14445.1 vs. uniprot
Match: V4NNF9_EUTSA (Uncharacterized protein n=1 Tax=Eutrema salsugineum TaxID=72664 RepID=V4NNF9_EUTSA)

HSP 1 Score: 71.2 bits (173), Expect = 1.660e-10
Identity = 37/86 (43.02%), Postives = 57/86 (66.28%), Query Frame = 1 
Query:  217 QLSPALYEFMGSKETMMARGDVTKAVWAYIRAASLQDPKDRRKIRNDDKMFRVFKCKTMNMMHIAKKLSPHLKKYSDLVVGDTESC 474
            QLSP L +F+G+ +  +AR +VTK +WAYI+   LQDPKDRRKI  D+ +  +F+ K++NM  ++K L+ H+       +GD + C
Sbjct:  125 QLSPQLEKFLGTSQ--LARTEVTKKLWAYIKEKDLQDPKDRRKILCDESLHSLFRVKSINMFQMSKALTKHIWP-----LGDGDGC 203
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BLAST of mRNA_L-elsbetiae_contig5954.14445.1 vs. uniprot
Match: UPI000CED48BA (DNA ligase 1 isoform X1 n=2 Tax=Eutrema salsugineum TaxID=72664 RepID=UPI000CED48BA)

HSP 1 Score: 71.2 bits (173), Expect = 2.100e-10
Identity = 37/86 (43.02%), Postives = 57/86 (66.28%), Query Frame = 1 
Query:  217 QLSPALYEFMGSKETMMARGDVTKAVWAYIRAASLQDPKDRRKIRNDDKMFRVFKCKTMNMMHIAKKLSPHLKKYSDLVVGDTESC 474
            QLSP L +F+G+ +  +AR +VTK +WAYI+   LQDPKDRRKI  D+ +  +F+ K++NM  ++K L+ H+       +GD + C
Sbjct:  125 QLSPQLEKFLGTSQ--LARTEVTKKLWAYIKEKDLQDPKDRRKILCDESLHSLFRVKSINMFQMSKALTKHIWP-----LGDGDGC 203
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BLAST of mRNA_L-elsbetiae_contig5954.14445.1 vs. uniprot
Match: A0A7N2MYS9_QUELO (Uncharacterized protein n=5 Tax=Fagaceae TaxID=3503 RepID=A0A7N2MYS9_QUELO)

HSP 1 Score: 70.5 bits (171), Expect = 4.110e-10
Identity = 37/71 (52.11%), Postives = 48/71 (67.61%), Query Frame = 1 
Query:  220 LSPALYEFMGSKETMMARGDVTKAVWAYIRAASLQDPKDRRKIRNDDKMFRVFKCKTMNMMHIAKKLSPHL 432
            LSP L EF+G  E  +AR  V K VWAYIR  +LQDPK+RR IR D+ +  +F+ KT+NM  + K LS H+
Sbjct:  133 LSPKLQEFVGEPE--LARTQVVKKVWAYIRENNLQDPKNRRNIRCDESLHTLFRVKTINMFQMNKALSKHI 201
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BLAST of mRNA_L-elsbetiae_contig5954.14445.1 vs. uniprot
Match: UPI00098E01B3 (upstream activation factor subunit spp27-like n=1 Tax=Asparagus officinalis TaxID=4686 RepID=UPI00098E01B3)

HSP 1 Score: 69.3 bits (168), Expect = 5.170e-10
Identity = 44/108 (40.74%), Postives = 59/108 (54.63%), Query Frame = 1 
Query:  220 LSPALYEFMGSKETMMARGDVTKAVWAYIRAASLQDPKDRRKIRNDDKMFRVFKCKTMNMMHIAKKLSPHLKKYSDLVVGDTESCSSXXXXXXAAPKGEDEVDERELR 543
            LSP L EF+G  ET +AR +V K +WAYIR  SLQDP +RRKI  D++M  +F  K ++M  + K LS H+         D+E+ S         PK E E  +   R
Sbjct:  118 LSPQLQEFLG--ETELARTEVVKRIWAYIRENSLQDPSNRRKILCDERMQNLFNVKKIDMFQMNKALSKHIWPL------DSEAGSIKPAQKERKPKKEREASDESPR 217
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BLAST of mRNA_L-elsbetiae_contig5954.14445.1 vs. uniprot
Match: UPI00098E144D (upstream activation factor subunit UAF30-like isoform X1 n=3 Tax=Asparagus officinalis TaxID=4686 RepID=UPI00098E144D)

HSP 1 Score: 69.3 bits (168), Expect = 5.630e-10
Identity = 36/71 (50.70%), Postives = 49/71 (69.01%), Query Frame = 1 
Query:  220 LSPALYEFMGSKETMMARGDVTKAVWAYIRAASLQDPKDRRKIRNDDKMFRVFKCKTMNMMHIAKKLSPHL 432
            LSP L EF+G  ET +AR +V K +WAYIR  SLQDP +RRKI  D++M  +F  K+++M  + K LS H+
Sbjct:  131 LSPQLQEFLG--ETELARTEVVKRIWAYIRENSLQDPSNRRKILCDERMHNLFNVKSIDMFQMNKALSKHI 199
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BLAST of mRNA_L-elsbetiae_contig5954.14445.1 vs. uniprot
Match: A0A7C9AVY7_OPUST (Uncharacterized protein n=2 Tax=Opuntia streptacantha TaxID=393608 RepID=A0A7C9AVY7_OPUST)

HSP 1 Score: 68.2 bits (165), Expect = 6.190e-10
Identity = 35/71 (49.30%), Postives = 47/71 (66.20%), Query Frame = 1 
Query:  220 LSPALYEFMGSKETMMARGDVTKAVWAYIRAASLQDPKDRRKIRNDDKMFRVFKCKTMNMMHIAKKLSPHL 432
            LSP L EF+G  E  +AR +V K +W YIR   LQDPK+RR I+ DDK+  +F+  T+NM  + K LS H+
Sbjct:   42 LSPQLQEFVGVPE--LARTEVVKKLWEYIREKDLQDPKNRRNIKCDDKLHSIFRVDTINMFQMNKALSKHI 110
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BLAST of mRNA_L-elsbetiae_contig5954.14445.1 vs. uniprot
Match: UPI00053C9E86 (golgin subfamily A member 6-like protein 22 isoform X1 n=5 Tax=Tarenaya hassleriana TaxID=28532 RepID=UPI00053C9E86)

HSP 1 Score: 70.1 bits (170), Expect = 6.880e-10
Identity = 36/78 (46.15%), Postives = 52/78 (66.67%), Query Frame = 1 
Query:  214 FQLSPALYEFMGSKETMMARGDVTKAVWAYIRAASLQDPKDRRKIRNDDKMFRVFKCKTMNMMHIAKKLSPHLKKYSD 447
            +QLSP L  F+GS +  +AR +V K +WAYIR  +LQDPK++R I  D+ +  +FK K +NM  ++K LS H+   SD
Sbjct:  123 WQLSPQLENFIGSSQ--LARTEVVKKIWAYIREKNLQDPKNKRNIICDESLHSLFKVKAINMFQMSKALSKHIWPLSD 198
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BLAST of mRNA_L-elsbetiae_contig5954.14445.1 vs. uniprot
Match: A0A7J9GPB7_9ROSI (Uncharacterized protein n=1 Tax=Gossypium harknessii TaxID=34285 RepID=A0A7J9GPB7_9ROSI)

HSP 1 Score: 67.4 bits (163), Expect = 8.630e-10
Identity = 38/106 (35.85%), Postives = 60/106 (56.60%), Query Frame = 1 
Query:  220 LSPALYEFMGSKETMMARGDVTKAVWAYIRAASLQDPKDRRKIRNDDKMFRVFKCKTMNMMHIAKKLSPHLKKYSDLVVGDTESCSSXXXXXXAAPKGEDEVDERE 537
            LSP L  F+G  E  +AR +V K +W YIR  +LQDPKD+R +  DD ++ +F+ K++NM  + K LS H+   ++  V   +S  +      A  +  DE  ++E
Sbjct:   21 LSPQLQAFIGESE--LARTEVVKRLWTYIREKNLQDPKDKRNVLCDDSLYALFRVKSINMFQMNKALSKHIWPLNEEEV-QADSAKTERKSKQARERVADEPKQKE 123
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig5954.14445.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5L1F8_9PHAE2.890e-2659.77Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G2V1_ECTSI1.030e-2594.83SWIB/MDM2 domain-containing protein n=1 Tax=Ectoca... [more]
V4NNF9_EUTSA1.660e-1043.02Uncharacterized protein n=1 Tax=Eutrema salsugineu... [more]
UPI000CED48BA2.100e-1043.02DNA ligase 1 isoform X1 n=2 Tax=Eutrema salsugineu... [more]
A0A7N2MYS9_QUELO4.110e-1052.11Uncharacterized protein n=5 Tax=Fagaceae TaxID=350... [more]
UPI00098E01B35.170e-1040.74upstream activation factor subunit spp27-like n=1 ... [more]
UPI00098E144D5.630e-1050.70upstream activation factor subunit UAF30-like isof... [more]
A0A7C9AVY7_OPUST6.190e-1049.30Uncharacterized protein n=2 Tax=Opuntia streptacan... [more]
UPI00053C9E866.880e-1046.15golgin subfamily A member 6-like protein 22 isofor... [more]
A0A7J9GPB7_9ROSI8.630e-1035.85Uncharacterized protein n=1 Tax=Gossypium harkness... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig5954contigL-elsbetiae_contig5954:5905..7231 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score112.1
Seed ortholog evalue3e-22
Seed eggNOG ortholog2880.D7G2V1
KEGG koko:K15223
Hectar predicted targeting categoryother localisation
GOsGO:0000120,GO:0000500,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005667,GO:0005730,GO:0006355,GO:0006356,GO:0008150,GO:0009889,GO:0009891,GO:0009893,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010628,GO:0019219,GO:0019222,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031974,GO:0031981,GO:0032991,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044452,GO:0044464,GO:0044798,GO:0045893,GO:0045935,GO:0045943,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0051171,GO:0051173,GO:0051252,GO:0051254,GO:0060255,GO:0065007,GO:0070013,GO:0080090,GO:1902680,GO:1903506,GO:1903508,GO:2000112,GO:2001141
EggNOG free text desc.SWIB/MDM2 domain
EggNOG OGsCOG5531@1,KOG1946@2759
COG Functional cat.B
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03021
Ec32 ortholog descriptionSWIB/MDM2 domain
Ec32 orthologEc-07_005610.1
Exons2
Model size811
Cds size591
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig5954.14445.1prot_L-elsbetiae_contig5954.14445.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig5954 5956..7062 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816644.6308742-UTR-L-elsbetiae_contig5954:5904..59551622816644.6308742-UTR-L-elsbetiae_contig5954:5904..5955Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5954 5905..5955 +
1691683708.0785315-UTR-L-elsbetiae_contig5954:5904..59551691683708.0785315-UTR-L-elsbetiae_contig5954:5904..5955Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5954 5905..5955 +
1622816644.7391183-UTR-L-elsbetiae_contig5954:7062..72311622816644.7391183-UTR-L-elsbetiae_contig5954:7062..7231Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5954 7063..7231 +
1691683708.1332831-UTR-L-elsbetiae_contig5954:7062..72311691683708.1332831-UTR-L-elsbetiae_contig5954:7062..7231Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5954 7063..7231 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816644.7118747-CDS-L-elsbetiae_contig5954:5955..61831622816644.7118747-CDS-L-elsbetiae_contig5954:5955..6183Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5954 5956..6183 +
1691683708.1020494-CDS-L-elsbetiae_contig5954:5955..61831691683708.1020494-CDS-L-elsbetiae_contig5954:5955..6183Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5954 5956..6183 +
1622816644.7270577-CDS-L-elsbetiae_contig5954:6699..70621622816644.7270577-CDS-L-elsbetiae_contig5954:6699..7062Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5954 6700..7062 +
1691683708.121333-CDS-L-elsbetiae_contig5954:6699..70621691683708.121333-CDS-L-elsbetiae_contig5954:6699..7062Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5954 6700..7062 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig5954.14445.1

>prot_L-elsbetiae_contig5954.14445.1 ID=prot_L-elsbetiae_contig5954.14445.1|Name=mRNA_L-elsbetiae_contig5954.14445.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=197bp
MLEGEGDGEDQEEEEGEEQEEEEEEEEEEEEEEEEEEGEEPAAVKAKAKT
KRTDFQLSPALYEFMGSKETMMARGDVTKAVWAYIRAASLQDPKDRRKIR
NDDKMFRVFKCKTMNMMHIAKKLSPHLKKYSDLVVGDTESCSSSSSSSSA
APKGEDEVDERELRLPWVCNNYCACSLAVLGGMSDSVCGEPRKTRR*
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mRNA from alignment at L-elsbetiae_contig5954:5905..7231+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig5954.14445.1 ID=mRNA_L-elsbetiae_contig5954.14445.1|Name=mRNA_L-elsbetiae_contig5954.14445.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1327bp|location=Sequence derived from alignment at L-elsbetiae_contig5954:5905..7231+ (Laminarionema elsbetiae ELsaHSoW15)
GTGAATCTCTCCGAGAGGAAGGCGTTCTTGAAAGAAAAGGTGACCGCAAT
CATGTTGGAAGGTGAGGGGGACGGTGAGGACCAGGAAGAAGAGGAGGGAG
AGGAGCAGGAAGAGGAGGAGGAGGAAGAAGAGGAGGAGGAGGAGGAGGAG
GAGGAGGAGGAGGGGGAGGAGCCAGCTGCCGTGAAGGCCAAGGCCAAGAC
AAAGCGCACCGATTTCCAGCTGTCTCCGGCACTTTACGAGTTCATGGGCA
GCAAGGAGACCATGATGGCGCGCGGGGATGTGAGTGAGGAGGCCGGATGA
AAAACCCGACGCGCGACCACAAAACCAACCCAAAACAATCGCGGTTATTA
CCCTCAAATGGCGGGCCAAAGAACAAAAACGAATCGTCCACACGGTGCAG
AGGAAGTCCGCGCTCATCGCACGAACCACACCACACTTGTGTACCACGAG
TAATAGTATGAAAGTGAGACGCATGCGTGGGCAGGAGGGAGGAGGAAGGT
CGAGCACACCAAAACAAAATCAGGCTCCTGTGTTTACCCGTTTCTTATCC
TGTGTGACGCAGGTTTGATACGCCTTTGTAGACGCCAAACTGCAACGCGT
AGTCTTTTAGACCACCAGCAGGTACACGAAAGAAAAATAAACACACACGC
ACACAGTAGCACAACACACACACACACAAACGCATGCGTGTGTGTTCAGC
CAACTCGTTTTTGAGGTTTTCCAAGTATACTGAGTATTCAACAACCAAGG
TCAGATTGCTGACTTCGCCTCCCAACACCACGAAATGAACGCCAGGTAAC
CAAGGCCGTGTGGGCCTACATTCGCGCGGCGTCCCTCCAAGACCCCAAGG
ACAGGCGTAAGATCAGGAACGACGACAAGATGTTCCGCGTGTTCAAGTGC
AAGACGATGAACATGATGCACATCGCCAAGAAGCTGTCGCCGCACCTCAA
GAAGTACTCCGACCTCGTAGTCGGCGACACCGAGAGTTGCAGCAGCAGCA
GCAGCAGCAGCAGCGCGGCACCGAAAGGCGAGGATGAGGTCGACGAGCGT
GAGCTTCGCCTTCCGTGGGTTTGCAATAACTACTGTGCCTGTTCACTTGC
GGTTCTTGGCGGCATGTCTGATTCTGTCTGTGGCGAACCGCGCAAGACAC
GACGGTAAAAATAAAGATTTCTGTCAAGAGGGCGAGAGAGACGCCGGCAG
GGTTGTGCGGTCTTACTGCTGAGCTCGACAGCAGTGCGTACGAATGGGAC
ATGGCTTTTTTCAGGCTGTGGCCCGCGCATGTGGATGGTGCTTTTGCGCC
GGTGTCGTTTGCTGCCGTTGTTAGTCC
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Coding sequence (CDS) from alignment at L-elsbetiae_contig5954:5905..7231+

>mRNA_L-elsbetiae_contig5954.14445.1 ID=mRNA_L-elsbetiae_contig5954.14445.1|Name=mRNA_L-elsbetiae_contig5954.14445.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=1182bp|location=Sequence derived from alignment at L-elsbetiae_contig5954:5905..7231+ (Laminarionema elsbetiae ELsaHSoW15)
ATGTTGGAAGGTGAGGGGGACGGTGAGGACCAGGAAGAAGAGGAGGGAGA
GGAGCAGGAAGAGGAGGAGGAGGAAGAAGAGGAGGAGGAGGAGGAGGAGG
AGGAGGAGGAGGGGGAGGAGCCAGCTGCCGTGAAGGCCAAGGCCAAGACA
AAGCGCACCGATTTCCAGCTGTCTCCGGCACTTTACGAGTTCATGGGCAG
CAAGGAGACCATGATGGCGCGCGGGGATATGTTGGAAGGTGAGGGGGACG
GTGAGGACCAGGAAGAAGAGGAGGGAGAGGAGCAGGAAGAGGAGGAGGAG
GAAGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGGGGAGGAGCC
AGCTGCCGTGAAGGCCAAGGCCAAGACAAAGCGCACCGATTTCCAGCTGT
CTCCGGCACTTTACGAGTTCATGGGCAGCAAGGAGACCATGATGGCGCGC
GGGGATGTAACCAAGGCCGTGTGGGCCTACATTCGCGCGGCGTCCCTCCA
AGACCCCAAGGACAGGCGTAAGATCAGGAACGACGACAAGATGTTCCGCG
TGTTCAAGTGCAAGACGATGAACATGATGCACATCGCCAAGAAGCTGTCG
CCGCACCTCAAGAAGTACTCCGACCTCGTAGTCGGCGACACCGAGAGTTG
CAGCAGCAGCAGCAGCAGCAGCAGCGCGGCACCGAAAGGCGAGGATGAGG
TCGACGAGCGTGAGCTTCGCCTTCCGTGGGTTTGCAATAACTACTGTGCC
TGTTCACTTGCGGTTCTTGGCGGCATGTCTGATTCTGTCTGTGGCGAACC
GCGCAAGACACGACGGTAAGTAACCAAGGCCGTGTGGGCCTACATTCGCG
CGGCGTCCCTCCAAGACCCCAAGGACAGGCGTAAGATCAGGAACGACGAC
AAGATGTTCCGCGTGTTCAAGTGCAAGACGATGAACATGATGCACATCGC
CAAGAAGCTGTCGCCGCACCTCAAGAAGTACTCCGACCTCGTAGTCGGCG
ACACCGAGAGTTGCAGCAGCAGCAGCAGCAGCAGCAGCGCGGCACCGAAA
GGCGAGGATGAGGTCGACGAGCGTGAGCTTCGCCTTCCGTGGGTTTGCAA
TAACTACTGTGCCTGTTCACTTGCGGTTCTTGGCGGCATGTCTGATTCTG
TCTGTGGCGAACCGCGCAAGACACGACGGTAA
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