mRNA_L-elsbetiae_contig5933.14414.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig5933.14414.1
Unique NamemRNA_L-elsbetiae_contig5933.14414.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig5933.14414.1 vs. uniprot
Match: D8LGI7_ECTSI (TIP120 domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LGI7_ECTSI)

HSP 1 Score: 219 bits (557), Expect = 7.160e-64
Identity = 112/116 (96.55%), Postives = 115/116 (99.14%), Query Frame = 1
Query:    1 SQHERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSEALLNRLAVHLLQKISESPPADVVRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGNPEDESLHTEAADELRENCFQ 348
            S+HERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSE+LLNRLAVHLLQKISESPP DVVRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCG+PEDESLHTEAADELRENCFQ
Sbjct:   64 SEHERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSESLLNRLAVHLLQKISESPPPDVVRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGDPEDESLHTEAADELRENCFQ 179          
BLAST of mRNA_L-elsbetiae_contig5933.14414.1 vs. uniprot
Match: A0A835Z0R5_9STRA (Armadillo-type protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z0R5_9STRA)

HSP 1 Score: 154 bits (388), Expect = 8.290e-43
Identity = 77/115 (66.96%), Postives = 94/115 (81.74%), Query Frame = 1
Query:    4 QHERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSEALLNRLAVHLLQKISESPPADVVRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGNPEDESLHTEAADELRENCFQ 348
            +H R++  +L QL  ER VVRKRA  CLGS+AVV+S+ALLN+L VHLL +I  S  AD+VRTLIQ IGTISRTVG+RLGRHLD+IVPLF +F G+ +DE +HTEAA ELR+NCFQ
Sbjct:  164 EHNRVIEALLVQLGQERAVVRKRATACLGSIAVVLSDALLNKLVVHLLAQIKGSDRADIVRTLIQAIGTISRTVGFRLGRHLDAIVPLFLKFIGDMDDEDMHTEAASELRDNCFQ 278          
BLAST of mRNA_L-elsbetiae_contig5933.14414.1 vs. uniprot
Match: A0A485LBH5_9STRA (Aste57867_18631 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485LBH5_9STRA)

HSP 1 Score: 138 bits (347), Expect = 1.660e-35
Identity = 68/116 (58.62%), Postives = 88/116 (75.86%), Query Frame = 1
Query:    1 SQHERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSEALLNRLAVHLLQKISESPPADVVRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGNPEDESLHTEAADELRENCFQ 348
             +H  IM ++LK+L  +R  VRKR  +CLG++ VV S+ALL+RL  HLL  +     ++ VRTLIQTIGT+SRTVG+RLGRHL +IVPLF  FCG P+DESL  +A++ELRENCFQ
Sbjct:  165 GEHVAIMDLLLKELKDDRAFVRKRVTSCLGALGVVASDALLHRLMEHLLSSVKAEEDSNEVRTLIQTIGTLSRTVGHRLGRHLPTIVPLFLTFCGTPDDESLQNDASNELRENCFQ 280          
BLAST of mRNA_L-elsbetiae_contig5933.14414.1 vs. uniprot
Match: A0A6G0WW89_9STRA (TIP120 domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WW89_9STRA)

HSP 1 Score: 135 bits (340), Expect = 1.450e-34
Identity = 66/116 (56.90%), Postives = 88/116 (75.86%), Query Frame = 1
Query:    1 SQHERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSEALLNRLAVHLLQKISESPPADVVRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGNPEDESLHTEAADELRENCFQ 348
             +H  IM ++LK+L  ER  VRKR   CLG++ VV S+ALL+RL  HLL  + +   ++ VRTLIQTIGT+SR VG+RLGRHL +IVPLF +FCG+ +DES+  +A++ELRENCFQ
Sbjct:  165 GEHVAIMDLLLKELKDERAFVRKRVTACLGALGVVASDALLHRLVEHLLSSVKQEGDSNEVRTLIQTIGTLSRAVGHRLGRHLPTIVPLFLKFCGSTDDESMQNDASNELRENCFQ 280          
BLAST of mRNA_L-elsbetiae_contig5933.14414.1 vs. uniprot
Match: A0A024TMU3_9STRA (TIP120 domain-containing protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TMU3_9STRA)

HSP 1 Score: 135 bits (339), Expect = 1.980e-34
Identity = 67/118 (56.78%), Postives = 90/118 (76.27%), Query Frame = 1
Query:    1 SQHERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSEALLNRLAVHLLQKISESPPADV--VRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGNPEDESLHTEAADELRENCFQ 348
             +H  IM ++LK+L  +R  VRKR  +CLG++ VV ++ALL+RL  HLL  + ++  +D   VRTLIQTIGT+SRTVG+RLGRHL +IVPLF  FCG+P+DES+  + A+ELRENCFQ
Sbjct:  165 GEHVSIMDILLKELNDDRAFVRKRVTSCLGALGVVATDALLHRLVEHLLADVKQASSSDSSEVRTLIQTIGTLSRTVGHRLGRHLSTIVPLFLTFCGSPDDESMQNDTANELRENCFQ 282          
BLAST of mRNA_L-elsbetiae_contig5933.14414.1 vs. uniprot
Match: A0A3R6ZTI8_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A3R6ZTI8_9STRA)

HSP 1 Score: 135 bits (339), Expect = 2.030e-34
Identity = 67/118 (56.78%), Postives = 90/118 (76.27%), Query Frame = 1
Query:    1 SQHERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSEALLNRLAVHLLQKISESPPADV--VRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGNPEDESLHTEAADELRENCFQ 348
             +H  IM ++LK+L  +R  VRKR  +CLG++ VV ++ALL+RL  HLL  + ++  +D   VRTLIQTIGT+SRTVG+RLGRHL +IVPLF  FCG+P+DES+  + A+ELRENCFQ
Sbjct:  169 GEHVSIMDILLKELNDDRAFVRKRVTSCLGALGVVATDALLHRLVEHLLADVKQASSSDSSEVRTLIQTIGTLSRTVGHRLGRHLSTIVPLFLTFCGSPDDESMQNDTANELRENCFQ 286          
BLAST of mRNA_L-elsbetiae_contig5933.14414.1 vs. uniprot
Match: A0A067BEA3_SAPPC (Uncharacterized protein (Fragment) n=1 Tax=Saprolegnia parasitica (strain CBS 223.65) TaxID=695850 RepID=A0A067BEA3_SAPPC)

HSP 1 Score: 134 bits (338), Expect = 2.410e-34
Identity = 68/116 (58.62%), Postives = 89/116 (76.72%), Query Frame = 1
Query:    1 SQHERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSEALLNRLAVHLLQKISESPPADVVRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGNPEDESLHTEAADELRENCFQ 348
            S+H  IM ++LK+L  ER  VRKR  +CLG++ VV ++ALL+RL  HLLQ +  +  AD  RTLIQTIGT+SR+VG+RLGRHL  IVPLF  FCG+P+DES+  + ++ELRENCFQ
Sbjct:  141 SEHVAIMDLLLKELSDERAFVRKRVTSCLGALGVVAADALLHRLVEHLLQSVENNHEADK-RTLIQTIGTLSRSVGHRLGRHLPVIVPLFLTFCGSPDDESMQNDVSNELRENCFQ 255          
BLAST of mRNA_L-elsbetiae_contig5933.14414.1 vs. uniprot
Match: T0R139_SAPDV (TIP120 domain-containing protein n=1 Tax=Saprolegnia diclina (strain VS20) TaxID=1156394 RepID=T0R139_SAPDV)

HSP 1 Score: 134 bits (338), Expect = 2.710e-34
Identity = 68/116 (58.62%), Postives = 89/116 (76.72%), Query Frame = 1
Query:    1 SQHERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSEALLNRLAVHLLQKISESPPADVVRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGNPEDESLHTEAADELRENCFQ 348
            S+H  IM ++LK+L  ER  VRKR  +CLG++ VV ++ALL+RL  HLLQ +  +  AD  RTLIQTIGT+SR+VG+RLGRHL  IVPLF  FCG+P+DES+  + ++ELRENCFQ
Sbjct:  164 SEHVAIMDLLLKELSDERAFVRKRVTSCLGALGVVAADALLHRLVEHLLQSVENNHEADK-RTLIQTIGTLSRSVGHRLGRHLPVIVPLFLTFCGSPDDESMQNDVSNELRENCFQ 278          
BLAST of mRNA_L-elsbetiae_contig5933.14414.1 vs. uniprot
Match: A0A1V9ZD09_9STRA (Cullin-associated NEDD8-dissociated protein (Fragment) n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZD09_9STRA)

HSP 1 Score: 132 bits (333), Expect = 1.320e-33
Identity = 69/116 (59.48%), Postives = 87/116 (75.00%), Query Frame = 1
Query:    1 SQHERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSEALLNRLAVHLLQKISESPPADVVRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGNPEDESLHTEAADELRENCFQ 348
            S+H  IM  +LK+L  ER  VRKR  TCLG++ VV S+ALL+RL  HLL  +  +  AD  RTLIQTIGT+SR+VG+RLGRHL  IVPLF QFCG+P +ES+  + ++ELRENCFQ
Sbjct: 2164 SEHVAIMDQLLKELSDERAFVRKRVTTCLGALGVVASDALLHRLVEHLLHSVETNHEADK-RTLIQTIGTLSRSVGHRLGRHLPVIVPLFLQFCGSPSNESMQNDNSNELRENCFQ 2278          
BLAST of mRNA_L-elsbetiae_contig5933.14414.1 vs. uniprot
Match: A0A1V9YGK7_9STRA (Cullin-associated NEDD8-dissociated protein n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9YGK7_9STRA)

HSP 1 Score: 131 bits (330), Expect = 3.250e-33
Identity = 66/116 (56.90%), Postives = 88/116 (75.86%), Query Frame = 1
Query:    1 SQHERIMTVVLKQLPHERVVVRKRAATCLGSVAVVVSEALLNRLAVHLLQKISESPPADVVRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGNPEDESLHTEAADELRENCFQ 348
            ++H  IM ++LK+L  ER  VRKR  +CLG++ +V ++ALL+RL  HLLQ +  +  AD  RTLIQTIGT+SR+VG+RLGRHL  IVPLF  FCG+P DES+  + ++ELRENCFQ
Sbjct:  181 TEHVAIMDLLLKELGDERAFVRKRVTSCLGALGIVAADALLHRLVEHLLQSVENNHNADK-RTLIQTIGTLSRSVGHRLGRHLPVIVPLFLTFCGDPSDESMQNDVSNELRENCFQ 295          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig5933.14414.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LGI7_ECTSI7.160e-6496.55TIP120 domain-containing protein n=2 Tax=Ectocarpu... [more]
A0A835Z0R5_9STRA8.290e-4366.96Armadillo-type protein (Fragment) n=1 Tax=Tribonem... [more]
A0A485LBH5_9STRA1.660e-3558.62Aste57867_18631 protein n=1 Tax=Aphanomyces stella... [more]
A0A6G0WW89_9STRA1.450e-3456.90TIP120 domain-containing protein n=1 Tax=Aphanomyc... [more]
A0A024TMU3_9STRA1.980e-3456.78TIP120 domain-containing protein n=1 Tax=Aphanomyc... [more]
A0A3R6ZTI8_9STRA2.030e-3456.78Uncharacterized protein (Fragment) n=1 Tax=Aphanom... [more]
A0A067BEA3_SAPPC2.410e-3458.62Uncharacterized protein (Fragment) n=1 Tax=Saprole... [more]
T0R139_SAPDV2.710e-3458.62TIP120 domain-containing protein n=1 Tax=Saprolegn... [more]
A0A1V9ZD09_9STRA1.320e-3359.48Cullin-associated NEDD8-dissociated protein (Fragm... [more]
A0A1V9YGK7_9STRA3.250e-3356.90Cullin-associated NEDD8-dissociated protein n=1 Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig5933contigL-elsbetiae_contig5933:10429..11264 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score209.9
Seed ortholog evalue5.9e-52
Seed eggNOG ortholog2880.D8LGI7
Preferred nameCAND1
KEGG koko:K09490,ko:K17263
KEGG TC1.A.33
KEGG Pathwayko03060,ko04141,ko04918,ko05020,map03060,map04141,map04918,map05020
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0000151,GO:0000909,GO:0003002,GO:0003006,GO:0005575,GO:0005618,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005829,GO:0005886,GO:0006464,GO:0006725,GO:0006807,GO:0007049,GO:0007275,GO:0007389,GO:0008150,GO:0008152,GO:0009404,GO:0009653,GO:0009712,GO:0009719,GO:0009725,GO:0009733,GO:0009791,GO:0009987,GO:0010033,GO:0010051,GO:0010228,GO:0010265,GO:0016020,GO:0016043,GO:0016567,GO:0016999,GO:0017144,GO:0018904,GO:0018940,GO:0018958,GO:0019538,GO:0019748,GO:0019953,GO:0022402,GO:0022414,GO:0022607,GO:0030154,GO:0030312,GO:0030435,GO:0030582,GO:0030584,GO:0032446,GO:0032501,GO:0032502,GO:0032991,GO:0034293,GO:0036211,GO:0042221,GO:0042537,GO:0043086,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043385,GO:0043412,GO:0043933,GO:0043934,GO:0043935,GO:0044085,GO:0044092,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0044703,GO:0048608,GO:0048646,GO:0048731,GO:0048856,GO:0048869,GO:0050790,GO:0050896,GO:0051321,GO:0051704,GO:0061458,GO:0065003,GO:0065007,GO:0065009,GO:0070647,GO:0071704,GO:0071840,GO:0071944,GO:0072490,GO:0075259,GO:1900570,GO:1900588,GO:1900591,GO:1900797,GO:1901360,GO:1901564,GO:1901615,GO:1902494,GO:1903046,GO:1990234
EggNOG free text desc.SCF complex assembly
EggNOG OGsKOG1824@1,KOG1824@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03110,ko04131,ko04147
Exons2
Model size348
Cds size330
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig5933.14414.1prot_L-elsbetiae_contig5933.14414.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig5933 10447..11264 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816642.2720013-UTR-L-elsbetiae_contig5933:10428..104461622816642.2720013-UTR-L-elsbetiae_contig5933:10428..10446Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5933 10429..10446 +
1691683706.5173473-UTR-L-elsbetiae_contig5933:10428..104461691683706.5173473-UTR-L-elsbetiae_contig5933:10428..10446Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5933 10429..10446 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816642.282628-CDS-L-elsbetiae_contig5933:10446..106261622816642.282628-CDS-L-elsbetiae_contig5933:10446..10626Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5933 10447..10626 +
1691683706.5298836-CDS-L-elsbetiae_contig5933:10446..106261691683706.5298836-CDS-L-elsbetiae_contig5933:10446..10626Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5933 10447..10626 +
1622816642.2928066-CDS-L-elsbetiae_contig5933:11114..112641622816642.2928066-CDS-L-elsbetiae_contig5933:11114..11264Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5933 11115..11264 +
1691683706.5379677-CDS-L-elsbetiae_contig5933:11114..112641691683706.5379677-CDS-L-elsbetiae_contig5933:11114..11264Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5933 11115..11264 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig5933.14414.1

>prot_L-elsbetiae_contig5933.14414.1 ID=prot_L-elsbetiae_contig5933.14414.1|Name=mRNA_L-elsbetiae_contig5933.14414.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=110bp
MTVVLKQLPHERVVVRKRAATCLGSVAVVVSEALLNRLAVHLLQKISESP
PADVVRTLIQTIGTISRTVGYRLGRHLDSIVPLFFQFCGNPEDESLHTEA
ADELRENCFQ
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mRNA from alignment at L-elsbetiae_contig5933:10429..11264+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig5933.14414.1 ID=mRNA_L-elsbetiae_contig5933.14414.1|Name=mRNA_L-elsbetiae_contig5933.14414.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=836bp|location=Sequence derived from alignment at L-elsbetiae_contig5933:10429..11264+ (Laminarionema elsbetiae ELsaHSoW15)
TCGCAGCACGAGCGCATCATGACGGTGGTCCTGAAGCAGCTTCCGCACGA GCGCGTGGTGGTGCGCAAGCGCGCGGCGACCTGCCTGGGGTCGGTGGCGG TCGTGGTCTCGGAGGCGCTGCTCAACCGGCTGGCGGTGCACCTGCTCCAG AAGATCTCGGAGTCGCCCCCGGCGGACGTCGTCCGCACGCTCATCCAGGT GAGCGGGGAGGGGAAGGGGAAGGGGAAGGGGAGGGGAAGGGAGGGGAGGG GAAGGGTATTAGGAGGTACTGGGTGGAGAGATCGGAACGGTTTTTGATGT GAAGAAAACAACACCCATCCGGGCAAGTGCGCCGAAACAAAAAACTAGCA ATAATAAACATGACGAGCCAACACACGAAGCTAAATTCGATTAACTTTTT TTCGCGGGGTGCTTGCACCCCCCCCCTCCCCCCCCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCTCTCCCCCCCCCCCCCCCC CCCCCCCCCCCCTCCCGGCGCCCCCGNNNNNNNAGCAATAATAACCATGA CGAGCCAACACACGAAGCCAATTTCGATTAACTTTTTTTCGCGGGGTGCT TGCCCCCCCCCCCCCCCCCCCCCCCTCTCGCTCGCCGCCGCCGCTGCGGC GCCCCTCTGGCTTTTTTTCGCGTCCACCGTCACCAGACGATCGGAACGAT CAGCCGCACCGTCGGCTACCGGCTCGGGAGACACCTGGACTCGATCGTGC CCCTGTTCTTCCAGTTCTGCGGCAACCCGGAGGACGAGAGCCTGCACACC GAGGCGGCGGACGAGCTCAGGGAGAACTGCTTCCAG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig5933:10429..11264+

>mRNA_L-elsbetiae_contig5933.14414.1 ID=mRNA_L-elsbetiae_contig5933.14414.1|Name=mRNA_L-elsbetiae_contig5933.14414.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=660bp|location=Sequence derived from alignment at L-elsbetiae_contig5933:10429..11264+ (Laminarionema elsbetiae ELsaHSoW15)
ATGACGGTGGTCCTGAAGCAGCTTCCGCACGAGCGCGTGGTGGTGCGCAA
GCGCGCGGCGACCTGCCTGGGGTCGGTGGCGGTCGTGGTCTCGGAGGCGC
TGCTCAACCGGCTGGCGGTGCACCTGCTCCAGAAGATCTCGGAGTCGCCC
CCGGCGGACGTCGTCCGCACGCTCATCCAGATGACGGTGGTCCTGAAGCA
GCTTCCGCACGAGCGCGTGGTGGTGCGCAAGCGCGCGGCGACCTGCCTGG
GGTCGGTGGCGGTCGTGGTCTCGGAGGCGCTGCTCAACCGGCTGGCGGTG
CACCTGCTCCAGAAGATCTCGGAGTCGCCCCCGGCGGACGTCGTCCGCAC
GCTCATCCAGACGATCGGAACGATCAGCCGCACCGTCGGCTACCGGCTCG
GGAGACACCTGGACTCGATCGTGCCCCTGTTCTTCCAGTTCTGCGGCAAC
CCGGAGGACGAGAGCCTGCACACCGAGGCGGCGGACGAGCTCAGGGAGAA
CTGCTTCCAGACGATCGGAACGATCAGCCGCACCGTCGGCTACCGGCTCG
GGAGACACCTGGACTCGATCGTGCCCCTGTTCTTCCAGTTCTGCGGCAAC
CCGGAGGACGAGAGCCTGCACACCGAGGCGGCGGACGAGCTCAGGGAGAA
CTGCTTCCAG
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