mRNA_L-elsbetiae_contig5451.13706.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig5451.13706.1
Unique NamemRNA_L-elsbetiae_contig5451.13706.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig5451.13706.1 vs. uniprot
Match: D8LSC5_ECTSI (EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSC5_ECTSI)

HSP 1 Score: 119 bits (297), Expect = 1.530e-28
Identity = 80/170 (47.06%), Postives = 103/170 (60.59%), Query Frame = 1
Query:   16 SGATLLVCSIQAWRSCSPRITRLPVGVGADTSTAVRLTNEGGLVIFNNTLLALADLYFSAKKINGKDATEKQLHSLQAMRRLLMRVEAVHAVSWLWASDARSVTHAAAEGTSRAAKATLNPLISMLPVLRRRTGRRRV----LLR--PCSGTR*SLDRDWLARRGSGSIS 507
            SG T L CSI+   S +PRI RL V  GADT++ VR+TN  G V +N+T LA+       K ++GK ATE+QLH LQA+RRLL+RV+AVHAVSWLW SDA S+ HA         +    PL   LP+L+RR     V    LLR  P S  R S   D L++  +G++S
Sbjct:   61 SGMTPLSCSIEWCSSGNPRIVRLLVDAGADTTSTVRVTNLNGGVEYNDTPLAVTTSLLREKVVDGKPATEEQLHRLQAIRRLLLRVDAVHAVSWLWPSDAPSIVHAVERSCGPEPRRP--PLSLTLPILKRRAKTCHVFVATLLRSSPISSGRGSRRAD-LSKEKAGTVS 227          
BLAST of mRNA_L-elsbetiae_contig5451.13706.1 vs. uniprot
Match: D7FVU0_ECTSI (EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVU0_ECTSI)

HSP 1 Score: 103 bits (257), Expect = 1.170e-21
Identity = 66/119 (55.46%), Postives = 77/119 (64.71%), Query Frame = 1
Query:   16 SGATLLVCSIQAWRSCSPRITRLPVGVGADTSTAVRLTNEGGLVIFNNTLLALADLYFSAKKI-NGKDATEKQLHSLQAMRRLLMRVEAVHAVSWLWASDARSVTHAAAEGTSRAAKAT 369
            SG T LV SIQ   S +PRI RL +  GADT++ +R TN  G V  N T LAL   Y   K + NG+ ATE+ L  L+A+RRLL+R EAVHAVSWLW SDA  V HAA E   RAA AT
Sbjct:  269 SGLTALVFSIQRCLSQTPRIVRLLINAGADTTSVIRGTNSEGEVQCNGTPLALTTSYLWEKILDNGEPATEEHLQRLEAIRRLLLRAEAVHAVSWLWPSDASFVGHAA-EKWPRAAFAT 386          
BLAST of mRNA_L-elsbetiae_contig5451.13706.1 vs. uniprot
Match: D7G1Y3_ECTSI (EsV-1-199 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G1Y3_ECTSI)

HSP 1 Score: 95.1 bits (235), Expect = 1.300e-18
Identity = 65/130 (50.00%), Postives = 76/130 (58.46%), Query Frame = 1
Query:   61 CSPRITRLPVGVGADTSTAVRLTNEGGLVIFNNTLLALADLYFSAKKINGKDATEKQLHSLQAMRRLLMRVEAVHAVSWLWASDAR-SVTHAAAEGTSRAAKATLNPLISMLPVLRRRTGRRRVLLRPCS 447
            C PRI RL V  GADT +AVR T   GLV F++T L          +  GKD  E+QL  L+A+RRLL+RVEAVHA SW+W S A  SV   AAE     A+     L S L +LRRR  RR VLLR  S
Sbjct:  242 CFPRIVRLLVDSGADTESAVRYTYRDGLVGFSDTPLCRTIRILRDVETEGKDDKEEQLGRLEAVRRLLLRVEAVHATSWVWPSSAAGSVAQDAAE-VKAGAETEPTRLTSTLQILRRRATRRGVLLRALS 370          
BLAST of mRNA_L-elsbetiae_contig5451.13706.1 vs. uniprot
Match: D7FT11_ECTSI (EsV-1-1 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FT11_ECTSI)

HSP 1 Score: 93.6 bits (231), Expect = 9.330e-18
Identity = 62/137 (45.26%), Postives = 83/137 (60.58%), Query Frame = 1
Query:   16 SGATLLVCSIQAWRSCSPRITRLPVGVGADTSTAVRLTNEGGLVIFNNTLLALADLYFSAKKI-NGKDATEKQLHSLQAMRRLLMRVEAVHAVSWLWASDARSVTHAAAEGTSRAAKATLNPLISMLPVLRRRTGRR 423
            +G T LV   Q   S +PRI R+ V  GADT++ V++ N  G V+FN+T LA   L    K + +GK AT++ L  L+A+ RLL+RV+AVHAVSWLW S A  +  A    T    +  L  L  MLP+L+RRT RR
Sbjct:  381 NGYTPLVVCTQGCFSQAPRIVRMLVDAGADTTSPVQVMNLKGGVVFNDTPLAFTKLRLRKKIVADGKPATKEHLQRLEAICRLLLRVDAVHAVSWLWPSGAPFIGRA----TETDPEPPLPALKLMLPILKRRTKRR 513          
BLAST of mRNA_L-elsbetiae_contig5451.13706.1 vs. uniprot
Match: A0A6H5KSA9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KSA9_9PHAE)

HSP 1 Score: 93.2 bits (230), Expect = 1.510e-17
Identity = 58/145 (40.00%), Postives = 91/145 (62.76%), Query Frame = 1
Query:   10 RPSGATLLVCSI-QAWRSCSPRITRLPVGVGADTSTAVRLTNEGGLVIFNNTLLALADLYFSAKKINGKDATEKQLHSLQAMRRLLMRVEAVHAVSWLWASDA-RSVTHAAAEGTSRA-AKATLNPLISMLPVLRRRTGRRRVLL 435
            +P G T +VC I +A RS SPR+ RL +  GADT++ VR+TN+ G+ +F  T LA        ++  G D+T+ +  SL+A+RR+L+++EA+ AVSW W SDA  S+  A  +G  RA ++ T++  + +    RR+   RRVL+
Sbjct:  445 QPDGRTPVVCRIGRALRSGSPRVLRLFLDAGADTASTVRITNKEGVQLFRGTPLAYTTWLLRDQQARGIDSTKGEPCSLEAVRRMLLQLEAIRAVSWCWQSDALSSIERAVQDGRPRAKSRLTVDTPLGLTLPRRRKARARRVLV 589          
BLAST of mRNA_L-elsbetiae_contig5451.13706.1 vs. uniprot
Match: A0A6H5KJA9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KJA9_9PHAE)

HSP 1 Score: 90.1 bits (222), Expect = 1.040e-16
Identity = 58/137 (42.34%), Postives = 78/137 (56.93%), Query Frame = 1
Query:   19 GATLLVCSIQAWRSCSPRITRLPVGVGADTSTAVRLTNE--GGLVIFNNTLLALADLYFSAKKINGKDATEKQLHSLQAMRRLLMRVEAVHAVSWLWASDARSVTHAAAEGTSRAAKATLNPLISMLPVLRRRTGRR 423
            G T L+  IQ +R   PR+ RL V  GADT++ V + N+  G  V      L +  +    K +    A+ +QLH L+A+RRLL+RVEAVHAVSWLW SD  ++T AAA GT R    +    + ML   RR  GR+
Sbjct:  289 GVTPLLWGIQHYR---PRVVRLLVEAGADTTSPVAVRNQLGGDDVSITPLGLTMNPVRDKVKVVGRGPASPEQLHKLEAIRRLLLRVEAVHAVSWLWPSDISTITRAAARGTDRTTAISTAVTLPMLVTRRRARGRK 422          
BLAST of mRNA_L-elsbetiae_contig5451.13706.1 vs. uniprot
Match: D7FVU3_ECTSI (EsV-1-1 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVU3_ECTSI)

HSP 1 Score: 88.6 bits (218), Expect = 3.010e-16
Identity = 59/140 (42.14%), Postives = 83/140 (59.29%), Query Frame = 1
Query:   19 GATLLVCSIQAWRS--CSPRITRLPVGVGADTSTAVRLTNEGGLVIFNNTLLALADLYFSAKKINGKDATEKQLHSLQAMRRLLMRVEAVHAVSWLWASDARSVTHAAAEGTSRAAKATLNPLISMLPVLRRRTGRRRVL 432
            G T L+ S +  ++  C+PRITR  V  GADT++AV+L  +      N T L   + +   +      AT++QL  L+A RR+L+RV AVHAVSW+W SDA  +   A   + R AK +   L ++LPVLRRR GRR +L
Sbjct:  258 GLTALLWSFRGNKNKPCNPRITRRLVDAGADTTSAVQLRAKRSRTCVNVTPLEYVNDWALRETNGDHPATDEQLLRLEATRRVLLRVVAVHAVSWIWPSDAPLLARPA--NSPRTAKHSPTQLTTVLPVLRRRAGRRALL 395          
BLAST of mRNA_L-elsbetiae_contig5451.13706.1 vs. uniprot
Match: D7FSU4_ECTSI (EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FSU4_ECTSI)

HSP 1 Score: 85.5 bits (210), Expect = 1.260e-15
Identity = 56/130 (43.08%), Postives = 80/130 (61.54%), Query Frame = 1
Query:   61 CSPRITRLPVGVGADTSTAVRLTNEGGLVIFNNTLLALADLYFSAKKINGKDA---TEKQLHSLQAMRRLLMRVEAVHAVSWLWASDARSVTHAAAEGTSR--AAKATLNPLISMLPVLRRRTGRRRVLL 435
            C PR+ +L V  GADT++AV ++   G         ++  L  + + ++G+     TE+QL  L+A+RRLL+RVEAVHAVSWLW++D  ++TH+ A GT R  A   T  PL+ M+   RRRT RR V L
Sbjct:  163 CRPRVGQLLVEAGADTTSAVPVSKHFGKD-------SVTPLGLTMRLLDGRSTYPLTEEQLKKLKAIRRLLLRVEAVHAVSWLWSTDISTITHSTAHGTDRTIATSTTGTPLMLMM---RRRTKRREVPL 282          
BLAST of mRNA_L-elsbetiae_contig5451.13706.1 vs. uniprot
Match: D7G1H7_ECTSI (EsV-1-199 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G1H7_ECTSI)

HSP 1 Score: 85.9 bits (211), Expect = 3.040e-15
Identity = 61/147 (41.50%), Postives = 85/147 (57.82%), Query Frame = 1
Query:   13 PSGATLLVCSIQ--AWRSCSPRITRLPVGVGADTSTAVRLTN-EGGLVIFNNTLLALADLYFSAKKINGKDATEKQLHSLQAMRRLLMRVEAVHAVSWLWAS-DARSVTHAAAEG-TSRAAKATLNPLISMLPVLRRRTGRRRVLLR 438
            P G T ++C++   A R CSP+I RL V  GA+T+ AV +T         + T LA         +++G+ AT++QL  L+  R LL R EAVHAVSWLWAS +   +  AA +  T  A+ A   PL +M P+LRR+ G R VLL+
Sbjct:  281 PYGRTPVMCAVDVGAGRLCSPKIVRLLVDAGANTTRAVGVTRITPRWEKTSETPLAFTTRLLREGRVDGEAATKEQLQRLKDTRCLLSRAEAVHAVSWLWASGEPPRIAQAAGDTKTETASTAVATPLRAMSPMLRRKPGTRGVLLK 427          
BLAST of mRNA_L-elsbetiae_contig5451.13706.1 vs. uniprot
Match: A0A6H5KQV0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KQV0_9PHAE)

HSP 1 Score: 85.5 bits (210), Expect = 4.390e-15
Identity = 59/146 (40.41%), Postives = 82/146 (56.16%), Query Frame = 1
Query:    1 DPLRPSGATLLVCSIQAWRSCSPRITRLPVGVGADTSTAVRLTNEGGLVIFNNTLLALADLYFSAKKINGKDATEKQLHSLQAMRRLLMRVEAVHAVSWLWASDARSVTHAAAEGTSR--AAKATLNPLISMLPVLRRRTGRRRVL 432
            + L   G T L+  IQ    C PR+ +L V  GADT++ V + N+ G   F+ T L L       +  + K  T ++ + L+ +RRLL+R EAVHAVSWLW SD  ++THA A+GT R  A      P I ML + RR  GR+ +L
Sbjct:  283 NTLNGVGLTSLLDGIQR---CRPRVVQLLVEAGADTTSPVLICNQPGEDDFHITPLGLTMCLLGGR--SAKPLTMEEQNKLEVIRRLLLREEAVHAVSWLWPSDTPTITHATAKGTGRTTATSTAATPFI-MLMIGRRTRGRKGLL 422          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig5451.13706.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LSC5_ECTSI1.530e-2847.06EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=288... [more]
D7FVU0_ECTSI1.170e-2155.46EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=288... [more]
D7G1Y3_ECTSI1.300e-1850.00EsV-1-199 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G1... [more]
D7FT11_ECTSI9.330e-1845.26EsV-1-1 n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
A0A6H5KSA9_9PHAE1.510e-1740.00Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5KJA9_9PHAE1.040e-1642.34Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FVU3_ECTSI3.010e-1642.14EsV-1-1 n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
D7FSU4_ECTSI1.260e-1543.08EsV-1-199 n=1 Tax=Ectocarpus siliculosus TaxID=288... [more]
D7G1H7_ECTSI3.040e-1541.50EsV-1-199 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G1... [more]
A0A6H5KQV0_9PHAE4.390e-1540.41Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig5451contigL-elsbetiae_contig5451:5186..6564 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score61.2
Seed ortholog evalue2.2e-07
Seed eggNOG ortholog2880.D7FSU4
Hectar predicted targeting categorysignal peptide
EggNOG free text desc.response to abiotic stimulus
EggNOG OGsCOG0666@1,KOG4177@2759
COG Functional cat.G
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons2
Model size884
Cds size210
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig5451.13706.1prot_L-elsbetiae_contig5451.13706.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig5451 5435..6139 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816571.648057-UTR-L-elsbetiae_contig5451:5185..54341622816571.648057-UTR-L-elsbetiae_contig5451:5185..5434Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5451 5186..5434 +
1691683655.6169796-UTR-L-elsbetiae_contig5451:5185..54341691683655.6169796-UTR-L-elsbetiae_contig5451:5185..5434Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5451 5186..5434 +
1622816571.6839402-UTR-L-elsbetiae_contig5451:6139..65641622816571.6839402-UTR-L-elsbetiae_contig5451:6139..6564Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5451 6140..6564 +
1691683655.663825-UTR-L-elsbetiae_contig5451:6139..65641691683655.663825-UTR-L-elsbetiae_contig5451:6139..6564Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5451 6140..6564 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816571.6601756-CDS-L-elsbetiae_contig5451:5434..56331622816571.6601756-CDS-L-elsbetiae_contig5451:5434..5633Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5451 5435..5633 +
1691683655.6326387-CDS-L-elsbetiae_contig5451:5434..56331691683655.6326387-CDS-L-elsbetiae_contig5451:5434..5633Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5451 5435..5633 +
1622816571.6731021-CDS-L-elsbetiae_contig5451:6128..61391622816571.6731021-CDS-L-elsbetiae_contig5451:6128..6139Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5451 6129..6139 +
1691683655.6522946-CDS-L-elsbetiae_contig5451:6128..61391691683655.6522946-CDS-L-elsbetiae_contig5451:6128..6139Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5451 6129..6139 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig5451.13706.1

>prot_L-elsbetiae_contig5451.13706.1 ID=prot_L-elsbetiae_contig5451.13706.1|Name=mRNA_L-elsbetiae_contig5451.13706.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=70bp
MRRLLMRVEAVHAVSWLWASDARSVTHAAAEGTSRAAKATLNPLISMLPV
LRRRTGRRRVLLRPCSGTR*
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mRNA from alignment at L-elsbetiae_contig5451:5186..6564+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig5451.13706.1 ID=mRNA_L-elsbetiae_contig5451.13706.1|Name=mRNA_L-elsbetiae_contig5451.13706.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1379bp|location=Sequence derived from alignment at L-elsbetiae_contig5451:5186..6564+ (Laminarionema elsbetiae ELsaHSoW15)
GACCCCCTCCGCCCATCCGGTGCAACGCTTTTGGTTTGTAGTATTCAGGC TTGGCGCTCGTGCTCTCCCAGAATCACGAGGTTGCCCGTGGGTGTAGGAG CGGACACTTCGACGGCCGTTCGCCTCACAAACGAAGGGGGTCTCGTGATT TTCAACAACACGCTCCTAGCTCTCGCGGACTTGTACTTCAGCGCCAAGAA GATCAATGGAAAGGACGCCACGGAGAAGCAACTACACAGCCTGCAGGCCA TGCGGCGGTTGCTGATGAGGGTGGAGGCTGTTCATGCCGTCTCTTGGCTG TGGGCAAGCGACGCCCGCTCCGTCACCCACGCTGCTGCAGAGGGTACGAG CAGGGCTGCCAAGGCAACTTTAAACCCGCTGATATCGATGTTGCCGGTCT TGAGGCGAAGAACTGGAAGGCGCAGGGTGCTCTTGCGACCCTGTTCAGGT GGATAGTGATGAACGTGATTTTTGGCTTCGCGCTTTGGCGCGATGCTTGC TAAACTTCATGTTGAGCCTTGGAGCAGTCTTGACCACTTGGGTTTGGTGT TGGGATTTCTGGCCACCTTCTCCTGCTGCTGCTGCTGCTGCTGCTGCTGC TGCTGCTGCTGCTGCTGCTACTGTTTGCCTTTGTACGTCGATTTCATGCG TGTATTCGCTACATTCGCTGGCTAAACGCGACAGCCATGCCACCTTCGTC CCACGCCGTGCGCAGTTTTGGGCACTGTAAATGGATGAATGGATACCCCA TATTTAATGGATTTACGCCACCGCTCCGGGAAGACAACATCCAGGAAAGC TTGCCATCTGGCCCTCACCTTACATTCTTGTTTTTTTCGGAAAATGCGCA GCAGTCGACCAACAGGCGCTCAAGCTCGCCCCGACCGACGCACCGTTTTG TTCCCCGTCTTGCCACACCCATCCTCGCATGCCGGTATTGAAGGAACTCG GTGAAGCCTTGACCGAGACTGGCTTGCGCGGCGTGGGAGTGGGAGCATTT CGTCGCTGAGAGTTGCATGGGGCCCTTTAGGGTGGATCGTACTGGATTTG GCCGCGCCTGTTTTGCATCCGCCCCAATGTGAAGAGGTAGAGGCTGCCAG AGGCCTCAAGTTTCAAGTGTTTGCGAGGAGCGATGGTGGCACCGATGGTG CATTTTTGCCCTTGGGGTCTATGATGGACAGAATTTACTCTTTCGGACCT TCCTTGAGTTCAATATGTTGCACCGAAAAATCAACAAAGGGAGGAGGAAA GATAAAGATGTGCGTGTGAACTGCGAATCAAGCTTTTAAAGGGTTGCGGG TAGCTCCAAGGCGGGAGGTCTCCTCGTGTTGACGCCTCAGTCCGAGATCG TCGTGAGTTGGCTGCGTGCCACGAAAACA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig5451:5186..6564+

>mRNA_L-elsbetiae_contig5451.13706.1 ID=mRNA_L-elsbetiae_contig5451.13706.1|Name=mRNA_L-elsbetiae_contig5451.13706.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=420bp|location=Sequence derived from alignment at L-elsbetiae_contig5451:5186..6564+ (Laminarionema elsbetiae ELsaHSoW15)
ATGCGGCGGTTGCTGATGAGGGTGGAGGCTGTTCATGCCGTCTCTTGGCT
GTGGGCAAGCGACGCCCGCTCCGTCACCCACGCTGCTGCAGAGGGTACGA
GCAGGGCTGCCAAGGCAACTTTAAACCCGCTGATATCGATGTTGCCGGTC
TTGAGGCGAAGAACTGGAAGGCGCAGGGTGCTCTTGCGACCCTGTTCAGA
TGCGGCGGTTGCTGATGAGGGTGGAGGCTGTTCATGCCGTCTCTTGGCTG
TGGGCAAGCGACGCCCGCTCCGTCACCCACGCTGCTGCAGAGGGTACGAG
CAGGGCTGCCAAGGCAACTTTAAACCCGCTGATATCGATGTTGCCGGTCT
TGAGGCGAAGAACTGGAAGGCGCAGGGTGCTCTTGCGACCCTGTTCAGGA
ACTCGGTGAGAACTCGGTGA
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