mRNA_L-elsbetiae_contig8289.17296.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig8289.17296.1
Unique NamemRNA_L-elsbetiae_contig8289.17296.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
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BLAST of mRNA_L-elsbetiae_contig8289.17296.1 vs. uniprot
Match: A0A6H5JP19_9PHAE (Uncharacterized protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JP19_9PHAE)

HSP 1 Score: 192 bits (488), Expect = 2.430e-50
Identity = 183/486 (37.65%), Postives = 265/486 (54.53%), Query Frame = 1 
Query:    1 IDATGCGQNSN--KNELCKKLVDGCKKVTQLLNQSARAKIQLEDSTAEEAANSDGEDGRVSKAKIAQPANQRLVSITKVLHVMLIKFPNLKAFWLDHRDTAFPLVDKHKELEEVFSILTEVKNTIECCQSTSKPVCLEAMQQLLSLCRSTLNLDKPLKISKVTP---EGGKEKPGALTVELRPVGDLSSTGLETRKAYREAVAARTVKLWYGDKYEENSFLLDMAMAMSPLGRPLNYLDALAGSSGKFGAENGYFTQAPQKIREKVWENIADLAEKSNTEERARKAATSGGNGTDGDXXXXXXXXXXXXXXXXXXXXXXXSSANPQLAAMADMGIFAPLPAEAGSAPSEETPRQEAERAVETWRNAVIPTEFAAALRGIDVCFFWKKHGDTICPQLKPVAQSILGLPPASAVLERDFCIADGFKTRKRSCVGPAYLEMMLFSRANYHLIPNRIPELDASQMKAAIPVRLTDPSKLGQVAQLDVLPE 1443
            ID TG    +N  KN  C+ L++ CKKV Q +N+S   KI+LED T E+A+     DG+  KAK++Q  +QR +S+ K+L  +L ++  +   +  +R+  FPL D  +E+EEV+S+L+E K  IE  Q+T +    +A++  + L   TL+  + L++  V     EG   K   +  E     DL+  G  TR A R+A+ AR +   YG + +    L D  M +SP  R L Y+DAL  S+ K G       ++   I+ K+ E + DL   +    RA  A + GG  +   XXXXXXXXXXXXXXXXXXXXXXX                    ++  + P EE PR+EAE  ++ W  A IP E     R  +   +W+ HG      L+ VAQ++ G+P ++ V+ERDF IAD F  RKR+ + PAYLEM L+ RA++  IP  IP+LD + + AAIP R  DP  + +V  LD   E
Sbjct:  170 IDGTGMANTANQSKNLSCRGLMEICKKVVQHVNRSQGFKIELED-TLEKAS-----DGKRWKAKLSQAVSQRWISLCKMLLRILEQWVAIDKVYASNREK-FPLGDLRREMEEVYSMLSEFKAVIEYSQTTHEATAQKALEMTVDLLTYTLDPAERLRVYHVVSIGAEGDTTKYDDIEHE-----DLTPIGRNTRVALRKALVARFLPR-YGRELDAGGHLFDRTMLLSPNQRKLRYVDALRSSAAKEGD-----MRSSSAIKAKIKEEVTDLLVAAIEAIRAT-APSDGGVESSAGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGSDSDMDATPVEEDPRKEAEAMMKEWLAAQIPPEE----RTENGAEYWRLHGKKHPFALRLVAQAVYGVPASAGVVERDFSIADLFMPRKRASLDPAYLEMQLYLRAHFDSIPMDIPKLDDASVAAAIPDRFRDPQAVEEVRVLDFTEE 632
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig8289.17296.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 1
Match NameE-valueIdentityDescription
A0A6H5JP19_9PHAE2.430e-5037.65Uncharacterized protein n=2 Tax=Ectocarpus sp. CCA... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig8289contigL-elsbetiae_contig8289:4478..8018 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score96.3
Seed ortholog evalue3.6e-17
Seed eggNOG ortholog2880.D7G3L2
Hectar predicted targeting categoryother localisation
EggNOG OGs2CZK4@1,2SARG@2759
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons4
Model size1485
Cds size1239
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig8289.17296.1prot_L-elsbetiae_contig8289.17296.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig8289 4484..6983 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816912.2872784-UTR-L-elsbetiae_contig8289:4477..44831622816912.2872784-UTR-L-elsbetiae_contig8289:4477..4483Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8289 4478..4483 -
1691683906.9626951-UTR-L-elsbetiae_contig8289:4477..44831691683906.9626951-UTR-L-elsbetiae_contig8289:4477..4483Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8289 4478..4483 -
1622816912.3301618-UTR-L-elsbetiae_contig8289:6983..71121622816912.3301618-UTR-L-elsbetiae_contig8289:6983..7112Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8289 6984..7112 -
1691683907.0114002-UTR-L-elsbetiae_contig8289:6983..71121691683907.0114002-UTR-L-elsbetiae_contig8289:6983..7112Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8289 6984..7112 -
1622816912.3426044-UTR-L-elsbetiae_contig8289:7907..80181622816912.3426044-UTR-L-elsbetiae_contig8289:7907..8018Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8289 7908..8018 -
1691683907.0224786-UTR-L-elsbetiae_contig8289:7907..80181691683907.0224786-UTR-L-elsbetiae_contig8289:7907..8018Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8289 7908..8018 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816912.297523-CDS-L-elsbetiae_contig8289:4483..48761622816912.297523-CDS-L-elsbetiae_contig8289:4483..4876Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8289 4484..4876 -
1691683906.977448-CDS-L-elsbetiae_contig8289:4483..48761691683906.977448-CDS-L-elsbetiae_contig8289:4483..4876Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8289 4484..4876 -
1622816912.3088138-CDS-L-elsbetiae_contig8289:5243..60051622816912.3088138-CDS-L-elsbetiae_contig8289:5243..6005Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8289 5244..6005 -
1691683906.9896765-CDS-L-elsbetiae_contig8289:5243..60051691683906.9896765-CDS-L-elsbetiae_contig8289:5243..6005Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8289 5244..6005 -
1622816912.3200712-CDS-L-elsbetiae_contig8289:6899..69831622816912.3200712-CDS-L-elsbetiae_contig8289:6899..6983Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8289 6900..6983 -
1691683907.0008357-CDS-L-elsbetiae_contig8289:6899..69831691683907.0008357-CDS-L-elsbetiae_contig8289:6899..6983Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8289 6900..6983 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig8289.17296.1

>prot_L-elsbetiae_contig8289.17296.1 ID=prot_L-elsbetiae_contig8289.17296.1|Name=mRNA_L-elsbetiae_contig8289.17296.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=413bp
MLIKFPNLKAFWLDHRDTAFPLVDKHKELEEVFSILTEVKNTIECCQSTS
KPVCLEAMQQLLSLCRSTLNLDKPLKISKVTPEGGKEKPGALTVELRPVG
DLSSTGLETRKAYREAVAARTVKLWYGDKYEENSFLLDMAMAMSPLGRPL
NYLDALAGSSGKFGAENGYFTQAPQKIREKVWENIADLAEKSNTEERARK
AATSGGNGTDGDGNGTGGDGNGTGGNGTSGGTQGASSANPQLAAMADMGI
FAPLPAEAGSAPSEETPRQEAERAVETWRNAVIPTEFAAALRGIDVCFFW
KKHGDTICPQLKPVAQSILGLPPASAVLERDFCIADGFKTRKRSCVGPAY
LEMMLFSRANYHLIPNRIPELDASQMKAAIPVRLTDPSKLGQVAQLDVLP
EAGGGGSDSDTD*
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mRNA from alignment at L-elsbetiae_contig8289:4478..8018-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig8289.17296.1 ID=mRNA_L-elsbetiae_contig8289.17296.1|Name=mRNA_L-elsbetiae_contig8289.17296.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=3541bp|location=Sequence derived from alignment at L-elsbetiae_contig8289:4478..8018- (Laminarionema elsbetiae ELsaHSoW15)
ATCGATGCCACTGGCTGCGGACAGAACAGCAACAAGAACGAGCTGTGCAA
GAAGTTGGTCGACGGCTGCAAGAAGGTGACTCAGCTTCTGAACCAGTCCG
CCAGGGCGAAGGTGAGCTTTAAACCTTTTGTTCATTGAACCGCAAAACTT
AAGAATTTTGTATCAGTGCTGGAACGGAAGGGGAGCTGTAAACATTTTTT
CGGAGCTGTAAACGTTTTTTCGGGAGAAAACGGCAGGGCCGGTGAAAACC
TCGGCGAAGAAGGAGCCCGGCGAAACTAGTAGGGGCACGAACATGGAGTC
GCCCCTTCAAGTACTTTGTATATACATATACATATACATATGTGACATGT
ATGCTAGCTGTAGTTGTGCAGAGCATCCCTTTTCGGGGCGCTGTCCCTGC
TTTTTTTGTATGGCGTTTGGGTTCATCTCAATTTCGAGTCCGCGTTGTTG
GGTCGGTACGCGCGATGAAAGGCCCCGAGAAGAAATATAAACGCTGCAAA
TTCGATGACAGCAAAAAAGCAGAAGAATGACAACACGCTGTTTTAGCCTG
TTCCCCATGTGTGAAAACGATGGAAGGGCGATGTTTTGTATTTTTGTTTT
TTGGTGGACGAAGGTAACACACAGAAACCAACATGAGCACTGCGGCCCAA
GTGAATTAACTTAGACGGGGGGTTCAAAATTTCGAAGATGAGTTGGGCGT
GGACTTGCAGCGGGGGCGGGGAGACGGAGGGCCCCCTGAAAAGATGCGGC
AGCTTTTCACCCTGCACAGGCGTCTTTCTCCCATCTGATAATCGAAAAGC
TCCGAGTCATCTGTGCACTCGTAGGATTTCCATCCGCGTGCGAACATAGC
AACGCACGTTTTAAGCTCGATCGTGGCCCCCTTATTTTTCCTTGCGCGCT
CACCAGATCCAGCTAGAGGATTCTACCGCGGAGGAAGCGGCTAACTCCGA
TGGCGAGGACGGGCGTGTCTCCAAGGCCAAGATCGCCCAGCCTGCCAACC
AACGCCTGGTCAGCATCACCAAAGTCCTCCACGTCATGCTCATCAAGTTC
CCCAACCTCAAGGCCTTTTGGCTTGACCACCGGGACACGGCCTTCCCCTT
GGTGGACAAGCACAAGGAGGTAAGTTCCGGATGACGGGATGAGAGGTCCC
TGTGAGGACCTGAAAGCGTATCCTTTCTATGTATGTATGCGTCACCACTC
GAGGGTGTTGCACCTACCATGAAAAAGTTGCAAATAAAACTCCTGTGTGA
TGCCGTTGAGGATGAGCTTTGCTGTGCATGCAGGTATGGTTCGTTCAGCC
AAAGATTTCCATATTTTTACGGATTCGTTCCTTTTGATTATTGCATACAA
ATGTCAGCAGCCTACGTCCAGTCTGTGAAAAAATCGTTCCAGAGAGAATT
GTTGTAAATAGCTTTTGAAAGATTTCGAGTTAGGTGAACTTCTAGCCATC
CTCCATGTAGCGTGCTATGTAGTGATCCTCACAACATCGCGAAAATATAT
ATCAAAAACTATGTTTTGCACATTCAGGGCACCCCTGCTCTATGTTCAAG
TCATTTTTGAGTCTGACACCAAAACAATATTGAAGACTGAGGAAAAGCTC
TGGGTTGTGCACGAAATGCTGCCAAAATTTCGAATATCTCGAGAACTGGA
GTTTGAATTCACAGTCGGAAAAAACTTACCCCTGGCGATTTTCGAGAAGA
ATCCATTGATGTGCTTCATTTTCAAATTGTACGGTTGGATTGTCCAAGAT
CAACTAAGCAAGTTTTGGCTCCCATGTTTTTGGAGCCGCGCAGGGAATGC
CCTCCTCTGAAAATGAGTGGCAACTACCGGTCAAGAAATGGCGTTTTGAA
GCTATGTATGTATGGACGAAAAGAGAAAGGTTTCCCGGATTGGAGCGTTT
GGAATTTGCAGCACTTTCGCTGTCCGATTGGTGGGCGCCCCCGCGGTTTT
AATAACTCCCCTTTTCTGTCCCGATCCTGTCCCATGTTTTGGTTTTACGT
CTATTCTTCGTAGTTGGAGGAAGTCTTCAGCATCCTGACTGAGGTAAAGA
ACACAATCGAGTGCTGCCAGTCCACCAGCAAGCCCGTGTGCCTCGAAGCG
ATGCAGCAACTCCTATCGCTCTGTCGTTCAACGCTGAACCTCGACAAACC
GCTCAAGATCTCCAAGGTCACGCCGGAAGGCGGGAAGGAGAAGCCTGGCG
CTTTGACGGTAGAGCTAAGACCGGTGGGGGACCTTTCGTCGACAGGCTTG
GAGACGCGGAAGGCGTACCGAGAAGCAGTGGCTGCGCGCACCGTTAAGCT
GTGGTACGGGGACAAGTACGAGGAGAACAGCTTTCTGCTGGACATGGCGA
TGGCAATGTCCCCCCTCGGCCGCCCGCTCAATTACCTGGATGCGCTCGCC
GGAAGCAGCGGAAAGTTCGGAGCAGAAAACGGCTATTTCACACAGGCCCC
TCAGAAGATCCGCGAGAAGGTTTGGGAAAATATTGCAGATCTCGCGGAGA
AATCGAACACCGAAGAACGAGCCCGCAAGGCAGCGACCTCAGGCGGCAAC
GGCACGGATGGTGACGGCAACGGTACGGGCGGTGATGGCAACGGCACGGG
CGGCAACGGCACCTCTGGAGGCACCCAGGGCGCTTCTTCTGCCAACCCCC
AGCTTGCGGCTATGGCCGATATGGGAATCTTTGCCCCTCTACCAGCCGAA
GCTGGAAGCGCACCGTCGGAGGAGACACCACGGCAGGAAGCAGAGAGAGC
GGTGGAAACCTGGAGGAATGCAGTGGTGAGCATTTGCCTGTGTCGTCTTC
GTTTTTTCCAAGCCCCTTCTTTGAAACATTGTTGCCGACTTAACCGCAAG
TAGGGCAGCGCTGCAGATCCGCTTTGTATGATTTCGTACGACTCTTGGTT
TCAGTGAGGGCGTCGGGTTAGCTTTGTTCACTTGTTGACTCCCCGACTTT
TAACCGTTCTACGCGAATCAGGCCATGGTGTCAAGCTGTGGGAATTGCGA
TCCTTGAGGTGGGATAGAAAAAAAACGCACGGTGGGAGAAACCACAACTA
AAATAATACATGCGCCCTGAATGCATTAATTCTCAATAGAAAAATAAAGC
GAAGCTCATGCTTGTGCACGACGTGTTTGTTACTTTGTCCAGATTCCGAC
CGAGTTTGCGGCGGCCTTGCGAGGAATCGATGTGTGTTTCTTTTGGAAGA
AGCACGGCGACACGATCTGCCCCCAACTGAAGCCCGTCGCGCAGTCCATT
CTTGGGCTCCCGCCTGCATCAGCTGTGCTCGAGCGAGACTTCTGCATCGC
AGACGGTTTTAAGACGAGGAAGCGGTCTTGTGTCGGCCCCGCTTACCTCG
AGATGATGCTGTTCTCTCGCGCAAACTACCACCTCATTCCCAACCGCATC
CCTGAGCTGGATGCATCCCAGATGAAGGCAGCGATCCCCGTGCGTCTGAC
TGACCCTTCGAAGCTGGGACAGGTGGCACAACTCGATGTCTTGCCGGAGG
CGGGGGGGGGCGGATCCGACTCGGACACGGACTAGACGGGA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig8289:4478..8018-

>mRNA_L-elsbetiae_contig8289.17296.1 ID=mRNA_L-elsbetiae_contig8289.17296.1|Name=mRNA_L-elsbetiae_contig8289.17296.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=2478bp|location=Sequence derived from alignment at L-elsbetiae_contig8289:4478..8018- (Laminarionema elsbetiae ELsaHSoW15)
ATGCTCATCAAGTTCCCCAACCTCAAGGCCTTTTGGCTTGACCACCGGGA
CACGGCCTTCCCCTTGGTGGACAAGCACAAGGAGATGCTCATCAAGTTCC
CCAACCTCAAGGCCTTTTGGCTTGACCACCGGGACACGGCCTTCCCCTTG
GTGGACAAGCACAAGGAGTTGGAGGAAGTCTTCAGCATCCTGACTGAGGT
AAAGAACACAATCGAGTGCTGCCAGTCCACCAGCAAGCCCGTGTGCCTCG
AAGCGATGCAGCAACTCCTATCGCTCTGTCGTTCAACGCTGAACCTCGAC
AAACCGCTCAAGATCTCCAAGGTCACGCCGGAAGGCGGGAAGGAGAAGCC
TGGCGCTTTGACGGTAGAGCTAAGACCGGTGGGGGACCTTTCGTCGACAG
GCTTGGAGACGCGGAAGGCGTACCGAGAAGCAGTGGCTGCGCGCACCGTT
AAGCTGTGGTACGGGGACAAGTACGAGGAGAACAGCTTTCTGCTGGACAT
GGCGATGGCAATGTCCCCCCTCGGCCGCCCGCTCAATTACCTGGATGCGC
TCGCCGGAAGCAGCGGAAAGTTCGGAGCAGAAAACGGCTATTTCACACAG
GCCCCTCAGAAGATCCGCGAGAAGGTTTGGGAAAATATTGCAGATCTCGC
GGAGAAATCGAACACCGAAGAACGAGCCCGCAAGGCAGCGACCTCAGGCG
GCAACGGCACGGATGGTGACGGCAACGGTACGGGCGGTGATGGCAACGGC
ACGGGCGGCAACGGCACCTCTGGAGGCACCCAGGGCGCTTCTTCTGCCAA
CCCCCAGCTTGCGGCTATGGCCGATATGGGAATCTTTGCCCCTCTACCAG
CCGAAGCTGGAAGCGCACCGTCGGAGGAGACACCACGGCAGGAAGCAGAG
AGAGCGGTGGAAACCTGGAGGAATGCAGTGTTGGAGGAAGTCTTCAGCAT
CCTGACTGAGGTAAAGAACACAATCGAGTGCTGCCAGTCCACCAGCAAGC
CCGTGTGCCTCGAAGCGATGCAGCAACTCCTATCGCTCTGTCGTTCAACG
CTGAACCTCGACAAACCGCTCAAGATCTCCAAGGTCACGCCGGAAGGCGG
GAAGGAGAAGCCTGGCGCTTTGACGGTAGAGCTAAGACCGGTGGGGGACC
TTTCGTCGACAGGCTTGGAGACGCGGAAGGCGTACCGAGAAGCAGTGGCT
GCGCGCACCGTTAAGCTGTGGTACGGGGACAAGTACGAGGAGAACAGCTT
TCTGCTGGACATGGCGATGGCAATGTCCCCCCTCGGCCGCCCGCTCAATT
ACCTGGATGCGCTCGCCGGAAGCAGCGGAAAGTTCGGAGCAGAAAACGGC
TATTTCACACAGGCCCCTCAGAAGATCCGCGAGAAGGTTTGGGAAAATAT
TGCAGATCTCGCGGAGAAATCGAACACCGAAGAACGAGCCCGCAAGGCAG
CGACCTCAGGCGGCAACGGCACGGATGGTGACGGCAACGGTACGGGCGGT
GATGGCAACGGCACGGGCGGCAACGGCACCTCTGGAGGCACCCAGGGCGC
TTCTTCTGCCAACCCCCAGCTTGCGGCTATGGCCGATATGGGAATCTTTG
CCCCTCTACCAGCCGAAGCTGGAAGCGCACCGTCGGAGGAGACACCACGG
CAGGAAGCAGAGAGAGCGGTGGAAACCTGGAGGAATGCAGTGATTCCGAC
CGAGTTTGCGGCGGCCTTGCGAGGAATCGATGTGTGTTTCTTTTGGAAGA
AGCACGGCGACACGATCTGCCCCCAACTGAAGCCCGTCGCGCAGTCCATT
CTTGGGCTCCCGCCTGCATCAGCTGTGCTCGAGCGAGACTTCTGCATCGC
AGACGGTTTTAAGACGAGGAAGCGGTCTTGTGTCGGCCCCGCTTACCTCG
AGATGATGCTGTTCTCTCGCGCAAACTACCACCTCATTCCCAACCGCATC
CCTGAGCTGGATGCATCCCAGATGAAGGCAGCGATCCCCGTGCGTCTGAC
TGACCCTTCGAAGCTGGGACAGGTGGCACAACTCGATGTCTTGCCGGAGG
CGGGGGGGGGCGGATCCGACTCGGACACGGACTAGATTCCGACCGAGTTT
GCGGCGGCCTTGCGAGGAATCGATGTGTGTTTCTTTTGGAAGAAGCACGG
CGACACGATCTGCCCCCAACTGAAGCCCGTCGCGCAGTCCATTCTTGGGC
TCCCGCCTGCATCAGCTGTGCTCGAGCGAGACTTCTGCATCGCAGACGGT
TTTAAGACGAGGAAGCGGTCTTGTGTCGGCCCCGCTTACCTCGAGATGAT
GCTGTTCTCTCGCGCAAACTACCACCTCATTCCCAACCGCATCCCTGAGC
TGGATGCATCCCAGATGAAGGCAGCGATCCCCGTGCGTCTGACTGACCCT
TCGAAGCTGGGACAGGTGGCACAACTCGATGTCTTGCCGGAGGCGGGGGG
GGGCGGATCCGACTCGGACACGGACTAG
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