mRNA_L-elsbetiae_contig8044.17033.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig8044.17033.1
Unique NamemRNA_L-elsbetiae_contig8044.17033.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig8044.17033.1 vs. uniprot
Match: D8LDZ2_ECTSI (Similar to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LDZ2_ECTSI)

HSP 1 Score: 96.7 bits (239), Expect = 4.030e-19
Identity = 65/151 (43.05%), Postives = 80/151 (52.98%), Query Frame = 3
Query:  105 PKILMMLLAIQGEDKRRHWATCS-AQGAPMLFWSAGYVSLAATHLLLAAGADEAISIR-GKHASCHIGSDLPDGALNSARAAGVARMLQRGPAFRARSWAWPALKAAGAGVDGKSSDMLPPGQNPNAILGVRVFRPR-KRFCMLRFVRYAS 548
            P+IL MLL ++GE KR  WA      G P L  +A   SL A  +LL AGADE  +    K ASC +G+ LP    N   +A V R+L+RGPAFRARSWAW A  +      G    M    +   A L VR+FRP   +F   RF RY  
Sbjct:  273 PRILQMLLDVEGEQKRVKWARLRHTDGTPALHLAAACESLVAASILLQAGADEGTANGLDKGASCCVGAALPANKKNPDTSAAVCRLLERGPAFRARSWAWAAESSEAPAASGTVCSM----KGGRATLDVRIFRPTGNQFFSARFSRYCK 419          
BLAST of mRNA_L-elsbetiae_contig8044.17033.1 vs. uniprot
Match: A0A6H5JK53_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JK53_9PHAE)

HSP 1 Score: 93.2 bits (230), Expect = 5.820e-18
Identity = 66/150 (44.00%), Postives = 85/150 (56.67%), Query Frame = 3
Query:  108 KILMMLLAIQGEDKRRHWATCSAQGAPMLFWSAGYVSLAATHLLLAAGADEAI-SIRGKHASCHIGSDLPDGALNSARA-AGVARMLQRGPAFRARSWAWPALKAAGAGVDGKSSDMLPPGQNPNAILGVRVFRPR-KRFCMLRFVRYAS 548
            KIL ML++ +GE+ RR WA C  +    L  +A +VS+ A H+LL+AGA E + +  GK AS  +G  L   A  S R  A V RMLQRGPA+RARS+ WP L     G +G S   L          GV++FRP   RF   RF RYA+
Sbjct:  255 KILEMLVSAEGEENRRKWAECRLRDGTPLHLAAAWVSVRAIHVLLSAGARETLPNGYGKLASEEVGIALRPPARRSPRKEAAVVRMLQRGPAYRARSYGWPTLD----GGNGTSDVTLSSRTRGANSFGVQIFRPTGTRFFATRFARYAN 400          
BLAST of mRNA_L-elsbetiae_contig8044.17033.1 vs. uniprot
Match: D7G4M4_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G4M4_ECTSI)

HSP 1 Score: 93.2 bits (230), Expect = 8.230e-18
Identity = 65/150 (43.33%), Postives = 83/150 (55.33%), Query Frame = 3
Query:  108 KILMMLLAIQGEDKRRHWATCSAQGAPMLFWSAGYVSLAATHLLLAAGADEAISIR-GKHASCHIGSDLPDGALNSARA-AGVARMLQRGPAFRARSWAWPALKAAGAGVDGKSSDMLPPGQNPNAILGVRVFRPR-KRFCMLRFVRYAS 548
            KIL ML++ +GED RR WA C  +    L  +A +VS+   H+LL+AGA E +  R GK A   +G  L   A  S R  A V RMLQRGPA+RARS+ WP L     G +G S   L          GV+++RP   RF   RF RYA+
Sbjct:  310 KILEMLVSAEGEDNRRKWAECRLRDGTPLHLAAAWVSVRVMHVLLSAGARETLPNRYGKLAGEEVGIALGPPARRSPRKEAAVVRMLQRGPAYRARSYGWPTLD----GGNGTSDVALSSRTRGAKSFGVQIYRPTGTRFFATRFARYAN 455          
BLAST of mRNA_L-elsbetiae_contig8044.17033.1 vs. uniprot
Match: D8LSK4_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LSK4_ECTSI)

HSP 1 Score: 84.0 bits (206), Expect = 1.070e-14
Identity = 50/118 (42.37%), Postives = 71/118 (60.17%), Query Frame = 3
Query:  108 KILMMLLAIQGEDKRRHWATCS-AQGAPMLFWSAGYVSLAATHLLLAAGADEAISIRGKHASCH-IGSDLPDGALNSARAAGVARMLQRGPAFRARSWAWPALKAAGAGVDGKSSDML 455
            +IL +LLA +G++++ HWA      G P+++ +A Y  LA   +LLAAGADE  +    +A+   IG  LP G L+   AA + R LQRGPA+RARSWAWP  + A    D  ++  L
Sbjct:  271 RILRLLLAAEGQERQEHWARRRLVLGRPLVYLAAAYGILANVKVLLAAGADETETDPDGYAAPEVIGGQLPSGTLDPLEAAAIGRELQRGPAYRARSWAWPVGEDAPVAGDAGAAGAL 388          
BLAST of mRNA_L-elsbetiae_contig8044.17033.1 vs. uniprot
Match: D8LSK8_ECTSI (Ankyrin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSK8_ECTSI)

HSP 1 Score: 80.9 bits (198), Expect = 1.810e-13
Identity = 44/104 (42.31%), Postives = 66/104 (63.46%), Query Frame = 3
Query:  111 ILMMLLAIQGEDKRRHWATCSAQGAPMLFWSAGYVSLAATHLLLAAGADE-AISIRGKHASCHIGSDLPDGALNSARAAGVARMLQRGPAFRARSWAWPALKAA 419
            I+ MLL+ +G ++  HWA C  +  P+L  +  + +++AT +LLAAGADE A +  G+ A   +G + P    + A+   + RML+RGPA+RARSWAWPA   A
Sbjct:  381 IVRMLLSAEGHEREGHWARCRYKEDPVLHSAVSFGNISATRVLLAAGADETAENEDGRPAREVVGFNAPSLQRDPAKKGAIVRMLERGPAYRARSWAWPATARA 484          
BLAST of mRNA_L-elsbetiae_contig8044.17033.1 vs. uniprot
Match: A0A6H5JSC7_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JSC7_9PHAE)

HSP 1 Score: 80.1 bits (196), Expect = 3.310e-13
Identity = 44/104 (42.31%), Postives = 65/104 (62.50%), Query Frame = 3
Query:  111 ILMMLLAIQGEDKRRHWATCSAQGAPMLFWSAGYVSLAATHLLLAAGADE-AISIRGKHASCHIGSDLPDGALNSARAAGVARMLQRGPAFRARSWAWPALKAA 419
            I+ MLL+ +G ++  HWA C  +  P+L  +  + +++AT +LLAAGADE A +  G+ A   +G + P    + A+   + RML RGPA+RARSWAWPA   A
Sbjct:  389 IVRMLLSAEGHEREGHWARCRYKEDPVLHCAVSFGNISATRVLLAAGADETAENEDGRPAREVVGFNAPSLQRDPAKKGAIVRMLARGPAYRARSWAWPATARA 492          
BLAST of mRNA_L-elsbetiae_contig8044.17033.1 vs. uniprot
Match: D8LMB2_ECTSI (Ankyrin repeat-containing protein 10_01 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LMB2_ECTSI)

HSP 1 Score: 74.3 bits (181), Expect = 1.360e-11
Identity = 55/141 (39.01%), Postives = 72/141 (51.06%), Query Frame = 3
Query:  111 ILMMLLAIQGEDKRRHWATCSAQGA---------------------PMLFWSAGYVSLAATHLLLAAGAD-EAISIRGKHASCHIGSDLPDGALNSARAAGVARMLQRGPAFRARSWAWPALKAAGAG---VDGKSSDMLP 458
            IL MLL ++G +K+  WA      A                     P+L ++A Y S+ A  +LL+AGAD E   ++GK A  HIGS LPD   N +  A V +MLQ+GPAFR RS AWP   A   G   V   SS+ +P
Sbjct:  151 ILEMLLNVEGGEKQEVWANQHVYFAHDGSLLRGVPGPTMATKFEWLPILHYAAKYCSIPALRILLSAGADTETCDLKGKLAVDHIGSLLPDDKRNGSMEAAVQQMLQKGPAFRVRSRAWPDTTATHGGASKVTQSSSNPVP 291          
BLAST of mRNA_L-elsbetiae_contig8044.17033.1 vs. uniprot
Match: D7FLL0_ECTSI (Ankyrin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FLL0_ECTSI)

HSP 1 Score: 60.5 bits (145), Expect = 9.240e-7
Identity = 46/120 (38.33%), Postives = 62/120 (51.67%), Query Frame = 3
Query:  108 KILMMLLAIQGEDKRRHWATCS-AQGAPMLFWSAGYVSLAATHLLLAAGADE-AISIRGKHASCHIGS-DLPDGALNS------------ARAAGVARMLQRGPAFRARSWAWPALKAAG 422
            KIL M+L I GE+    WA         +L +S GY+  A+   LLAAGA+E A    G+     IG+ DL +  L+              + A + R ++R PAFRARSWAWPA + AG
Sbjct:  232 KILRMMLNITGEENAARWADSDWGLAGSLLHYSCGYILPASVSALLAAGANENATDQAGQRPIDVIGTMDLQNPPLSPLHPRTPTRFRDPVKEAEIRRTVERAPAFRARSWAWPAFEWAG 351          
BLAST of mRNA_L-elsbetiae_contig8044.17033.1 vs. uniprot
Match: D8LME8_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LME8_ECTSI)

HSP 1 Score: 55.1 bits (131), Expect = 1.180e-5
Identity = 41/105 (39.05%), Postives = 56/105 (53.33%), Query Frame = 3
Query:  108 KILMMLL-AIQGEDKRRHWATCSAQGAPMLFWSAGYVSLAATHLLLAAGADEAISI-RGKHASCHIGSDLPDGALNSARAAGVARMLQRGPAFRARSWAWPALKA 416
            +IL MLL A+   +             P+L  +A +    ATH+LLAAGADE     RG+ A   IG  +PD   +  + A + RMLQ+G  FR+RS AWP  +A
Sbjct:   60 RILKMLLTAVHAGESDGPGVAEVVNSGPLLRLAALHGHPGATHVLLAAGADETRETDRGERARDLIGIFVPDCERDPEKEAAIGRMLQQGAVFRSRSLAWPTERA 164          
BLAST of mRNA_L-elsbetiae_contig8044.17033.1 vs. uniprot
Match: D8LMA6_ECTSI (Similar to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LMA6_ECTSI)

HSP 1 Score: 55.8 bits (133), Expect = 3.340e-5
Identity = 51/159 (32.08%), Postives = 73/159 (45.91%), Query Frame = 3
Query:  111 ILMMLLAIQGEDKRRHWAT----CS-----------------------AQGAPMLFWSAGYVSLAATHLLLAAGADEA-ISIRGKHASCHIGSDLPDGALNSARAAGVARMLQRGPAFRARSWAWPALKAAGAGVDGKSSDMLPPGQNPNAILGVRVFR 503
            +L +LL ++G++K+  WA     CS                       A G P+L  +A Y ++ A  +LL+AGA       +GK A   IG    +   N +  A V  +L +GPAFRARSWAWPA   A  G   + S  L P   P   +G + F+
Sbjct:  283 MLTILLNVEGDEKQEVWANQFVYCSHETVDVGLAPARDFPSTHAIMAIAFGCPLLHTAAKYCAIPAIRVLLSAGALAGKADYQGKVAEDAIGVYHHE--RNGSVEAAVRALLLKGPAFRARSWAWPATMNAALG---RPSRNLHPSSVPKTPMGWQTFQ 436          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig8044.17033.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 10
Match NameE-valueIdentityDescription
D8LDZ2_ECTSI4.030e-1943.05Similar to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus si... [more]
A0A6H5JK53_9PHAE5.820e-1844.00Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G4M4_ECTSI8.230e-1843.33Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D8LSK4_ECTSI1.070e-1442.37Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
D8LSK8_ECTSI1.810e-1342.31Ankyrin n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
A0A6H5JSC7_9PHAE3.310e-1342.31Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LMB2_ECTSI1.360e-1139.01Ankyrin repeat-containing protein 10_01 n=1 Tax=Ec... [more]
D7FLL0_ECTSI9.240e-738.33Ankyrin n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
D8LME8_ECTSI1.180e-539.05Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D8LMA6_ECTSI3.340e-532.08Similar to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus si... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig8044contigL-elsbetiae_contig8044:6568..7719 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score90.1
Seed ortholog evalue9.1e-16
Seed eggNOG ortholog2880.D8LDZ2
KEGG koko:K10380
KEGG Pathwayko04624,ko05205,map04624,map05205
Hectar predicted targeting categoryother localisation
EggNOG free text desc.response to abiotic stimulus
EggNOG OGsCOG0666@1,KOG4177@2759
COG Functional cat.G
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04812
Exons2
Model size857
Cds size438
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig8044.17033.1prot_L-elsbetiae_contig8044.17033.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig8044 6871..7603 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816889.363706-UTR-L-elsbetiae_contig8044:6567..68701622816889.363706-UTR-L-elsbetiae_contig8044:6567..6870Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8044 6568..6870 -
1691683889.556489-UTR-L-elsbetiae_contig8044:6567..68701691683889.556489-UTR-L-elsbetiae_contig8044:6567..6870Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8044 6568..6870 -
1622816889.3946195-UTR-L-elsbetiae_contig8044:7603..77191622816889.3946195-UTR-L-elsbetiae_contig8044:7603..7719Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8044 7604..7719 -
1691683889.5997472-UTR-L-elsbetiae_contig8044:7603..77191691683889.5997472-UTR-L-elsbetiae_contig8044:7603..7719Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig8044 7604..7719 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816889.3744113-CDS-L-elsbetiae_contig8044:6870..68861622816889.3744113-CDS-L-elsbetiae_contig8044:6870..6886Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8044 6871..6886 -
1691683889.572172-CDS-L-elsbetiae_contig8044:6870..68861691683889.572172-CDS-L-elsbetiae_contig8044:6870..6886Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8044 6871..6886 -
1622816889.3848252-CDS-L-elsbetiae_contig8044:7181..76031622816889.3848252-CDS-L-elsbetiae_contig8044:7181..7603Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8044 7182..7603 -
1691683889.584731-CDS-L-elsbetiae_contig8044:7181..76031691683889.584731-CDS-L-elsbetiae_contig8044:7181..7603Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig8044 7182..7603 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig8044.17033.1

>prot_L-elsbetiae_contig8044.17033.1 ID=prot_L-elsbetiae_contig8044.17033.1|Name=mRNA_L-elsbetiae_contig8044.17033.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=146bp
MMLLAIQGEDKRRHWATCSAQGAPMLFWSAGYVSLAATHLLLAAGADEAI
SIRGKHASCHIGSDLPDGALNSARAAGVARMLQRGPAFRARSWAWPALKA
AGAGVDGKSSDMLPPGQNPNAILGVRVFRPRKRFCMLRFVRYASI*
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mRNA from alignment at L-elsbetiae_contig8044:6568..7719-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig8044.17033.1 ID=mRNA_L-elsbetiae_contig8044.17033.1|Name=mRNA_L-elsbetiae_contig8044.17033.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1152bp|location=Sequence derived from alignment at L-elsbetiae_contig8044:6568..7719- (Laminarionema elsbetiae ELsaHSoW15)
AGGAGGCTCGTACGAAGTTTGCCGTACCCTGCTCAACGAAGGTTTCAAGG CCATCGGCGGGTTCGTCGCGGTCCCCAATGCCATGAAAAGGGCAATAGAA TGTGCCGAAAATCCTCATGATGTTGCTCGCCATCCAGGGAGAGGACAAGC GCAGGCATTGGGCGACGTGCTCGGCGCAGGGTGCGCCTATGCTATTTTGG TCCGCCGGGTACGTCTCTCTCGCTGCGACTCATCTTCTCCTGGCTGCTGG GGCGGACGAGGCAATCAGCATCCGCGGGAAACATGCAAGCTGCCATATTG GCTCAGATTTGCCAGACGGCGCGCTTAATTCAGCCAGGGCGGCCGGCGTC GCCCGAATGCTGCAGCGAGGACCAGCGTTTCGAGCGCGCTCGTGGGCGTG GCCAGCGCTGAAGGCTGCTGGTGCTGGTGTTGACGGTAAAAGTTCTGACA TGCTGCCTCCCGGTCAAAACCCAAACGCCATCTTGGGCGTACGTGTTTTC CGGCCGAGGAAGAGATTCTGCATGTTGCGCTTTGTCAGGTAAGACGGCAT GTCTGTCTAGGGGCGCTGTTTTTGTGAGGCTGGTGTACGTCCTTGGTCTA CTACTGCCACACTGGTTTACTGAACACGTCGGTAGGAAACGACTCCAGCG CAGACTTTTGGTTCCCTTTGCATAGCCAGGCAAATCTGCGAAGTTCACCG CCGGCAAACATTCTAGAGTTCTGGATTCTGGACAGGTCTTTACCTGTGTG ACCTGTACATCGATGCAAGCACTAGGTCTCGATCGACGTAGCAGCCGCTT CCTTTGTTCTCCAACCTCTCTCCCTCGTGTCAGGTACGCTAGTATTTAAT ACCGCGCAATGAGAACTTTCAAGATGCGTCTACGCGTGGTTTCGCAGCAG CACCAATCCCCAAAGTGGCAGCACTTGCGGCCGTGCTCTCTATGGGAAAG AGGAGAGTGCATGGGCAATAAGTGCTCGCGGGCTTGTGGTAGGTAGATAC GCTTATGCTTACATGACCCGTCGGGCCTCAAGGAGAAATTGTGTTCGTGA TGGCATACTGCCGTACTCCGTGGGATAATAGCAAATCTTATCTTTGCAGG GGAGATGTTGAATTCTTCTGATATTTAGGTTGTTTTTTCTGCTCGCTTCG CC
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Coding sequence (CDS) from alignment at L-elsbetiae_contig8044:6568..7719-

>mRNA_L-elsbetiae_contig8044.17033.1 ID=mRNA_L-elsbetiae_contig8044.17033.1|Name=mRNA_L-elsbetiae_contig8044.17033.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=876bp|location=Sequence derived from alignment at L-elsbetiae_contig8044:6568..7719- (Laminarionema elsbetiae ELsaHSoW15)
ATGATGTTGCTCGCCATCCAGGGAGAGGACAAGCGCAGGCATTGGGCGAC
GTGCTCGGCGCAGGGTGCGCCTATGCTATTTTGGTCCGCCGGGTACGTCT
CTCTCGCTGCGACTCATCTTCTCCTGGCTGCTGGGGCGGACGAGGCAATC
AGCATCCGCGGGAAACATGCAAGCTGCCATATTGGCTCAGATTTGCCAGA
CGGCGCGCTTAATTCAGCCAGGGCGGCCGGCGTCGCCCGAATGCTGCAGC
GAGGACCAGCGTTTCGAGCGCGCTCGTGGGCGTGGCCAGCGCTGAAGGCT
GCTGGTGCTGGTGTTGACGGTAAAAGTTCTGACATGCTGCCTCCCGGTCA
AAACCCAAACGCCATCTTGGGCGTACGTGTTTTCCGGCCGAGGAAGAGAT
TCTGCATGTTGCGCTTTGTCAGATGATGTTGCTCGCCATCCAGGGAGAGG
ACAAGCGCAGGCATTGGGCGACGTGCTCGGCGCAGGGTGCGCCTATGCTA
TTTTGGTCCGCCGGGTACGTCTCTCTCGCTGCGACTCATCTTCTCCTGGC
TGCTGGGGCGGACGAGGCAATCAGCATCCGCGGGAAACATGCAAGCTGCC
ATATTGGCTCAGATTTGCCAGACGGCGCGCTTAATTCAGCCAGGGCGGCC
GGCGTCGCCCGAATGCTGCAGCGAGGACCAGCGTTTCGAGCGCGCTCGTG
GGCGTGGCCAGCGCTGAAGGCTGCTGGTGCTGGTGTTGACGGTAAAAGTT
CTGACATGCTGCCTCCCGGTCAAAACCCAAACGCCATCTTGGGCGTACGT
GTTTTCCGGCCGAGGAAGAGATTCTGCATGTTGCGCTTTGTCAGGTACGC
TAGTATTTAAGTACGCTAGTATTTAA
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