mRNA_L-elsbetiae_contig7175.16010.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig7175.16010.1
Unique NamemRNA_L-elsbetiae_contig7175.16010.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig7175.16010.1 vs. uniprot
Match: A0A6H5J7N3_9PHAE (E1 ubiquitin-activating enzyme n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5J7N3_9PHAE)

HSP 1 Score: 252 bits (644), Expect = 1.990e-75
Identity = 120/145 (82.76%), Postives = 137/145 (94.48%), Query Frame = 1
Query:   10 QFYLTEADLGKPRAAACVAKLADLNRYVGVSTASGTVTESLVSSFQVVVMIDATLEEQLRVNDLCHQKGVCYISCDARGVFAYALCDFGDAFVVSDIDGNQAASCVVSSITKDAVGLVTVMDDQRHNLVTGDVVTFNSVQGMTEV 444
            QFYL+EADLG PRAAACV++LA+LNRYVGVST +G VTE+++ S+QVVVMIDA L+EQLRVND+CH KGVC+ISCDARGVFAYA CDFG++FVVSD DGNQAASCVVSS+TKDAVGLVTVMDDQRHNLVTGDVVTFNS+QGMTE+
Sbjct:  156 QFYLSEADLGNPRAAACVSRLAELNRYVGVSTTTGEVTEAMIGSYQVVVMIDAPLDEQLRVNDICHAKGVCFISCDARGVFAYAFCDFGESFVVSDTDGNQAASCVVSSVTKDAVGLVTVMDDQRHNLVTGDVVTFNSIQGMTEL 300          
BLAST of mRNA_L-elsbetiae_contig7175.16010.1 vs. uniprot
Match: A0A836CIM1_9STRA (Ubiquitin-activating enzyme E1 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CIM1_9STRA)

HSP 1 Score: 186 bits (473), Expect = 5.570e-52
Identity = 96/145 (66.21%), Postives = 110/145 (75.86%), Query Frame = 1
Query:   10 QFYLTEADLGKPRAAACVAKLADLNRYVGVSTASGTVTESLVSSFQVVVMIDATLEEQLRVNDLCHQKGVCYISCDARGVFAYALCDFGDAFVVSDIDGNQAASCVVSSITKDAVGLVTVMDDQRHNLVTGDVVTFNSVQGMTEV 444
            QFYLTEAD+GKPRAAACV KLA+LNRYV  S     +T +LVS FQVVV+ID  L  QL V+D CH KG+C I+ D RGVFA   CDFG AFVVSD +  QA SC+VS +TKD+  LVTVMDDQRH L TGDVVTF SV+GM E+
Sbjct:   68 QFYLTEADIGKPRAAACVNKLAELNRYV--SCVEEDLTTTLVSRFQVVVLIDVPLSVQLEVDDFCHSKGICCIAADVRGVFASVFCDFGPAFVVSDKNDTQAVSCLVSGVTKDSPALVTVMDDQRHQLETGDVVTFASVEGMPEL 210          
BLAST of mRNA_L-elsbetiae_contig7175.16010.1 vs. uniprot
Match: UPI0008541C9A (ubiquitin-like modifier-activating enzyme 1 isoform X1 n=2 Tax=Nanorana parkeri TaxID=125878 RepID=UPI0008541C9A)

HSP 1 Score: 161 bits (408), Expect = 3.480e-43
Identity = 76/145 (52.41%), Postives = 102/145 (70.34%), Query Frame = 1
Query:   10 QFYLTEADLGKPRAAACVAKLADLNRYVGVSTASGTVTESLVSSFQVVVMIDATLEEQLRVNDLCHQKGVCYISCDARGVFAYALCDFGDAFVVSDIDGNQAASCVVSSITKDAVGLVTVMDDQRHNLVTGDVVTFNSVQGMTEV 444
            QFYL E D+GK RA  C+  L +LNRYV VS ++  +++ L+SSFQ+V++ D+TLEEQLRV D CH  G+ YI  D RG+F    CDFG+ FVV D +G Q  S ++S ITKD+ G+VT +D+ RH   +GD VTF  +QGMTE+
Sbjct:  162 QFYLREGDIGKNRAEVCLTSLVELNRYVAVSASTKPLSDDLLSSFQLVILTDSTLEEQLRVGDFCHSHGIKYIVADTRGLFGQLFCDFGNDFVVLDPNGEQPLSAMISMITKDSNGVVTCLDEARHGFESGDFVTFTEIQGMTEL 306          
BLAST of mRNA_L-elsbetiae_contig7175.16010.1 vs. uniprot
Match: A0A7S3UZM0_9STRA (Hypothetical protein n=1 Tax=Aplanochytrium stocchinoi TaxID=215587 RepID=A0A7S3UZM0_9STRA)

HSP 1 Score: 161 bits (407), Expect = 4.680e-43
Identity = 79/146 (54.11%), Postives = 109/146 (74.66%), Query Frame = 1
Query:   10 QFYLTEADLGKPRAAACVAKLADLNRYVGVSTASGTVTE-SLVSSFQVVVMIDATLEEQLRVNDLCHQKGVCYISCDARGVFAYALCDFGDAFVVSDIDGNQAASCVVSSITKDAVGLVTVMDDQRHNLVTGDVVTFNSVQGMTEV 444
            QFYL E D+GKPRA AC+ KL +LN YV V+  +G++ + + ++SF VVVM+DA+LE +++VND+CH   V +ISC   G+FA+A CDFG  FVV+D +G QA S +V+SIT++  GLVTV D+ RH L  GD VTF+ V+GMTE+
Sbjct:   80 QFYLMEQDVGKPRAEACLNKLKELNEYVDVTHKAGSILDQAFITSFDVVVMVDASLETRVQVNDICHAANVRFISCQVAGLFAHAFCDFGQDFVVADTNGEQAISRLVTSITQEKEGLVTVHDETRHGLEDGDFVTFSEVKGMTEL 225          
BLAST of mRNA_L-elsbetiae_contig7175.16010.1 vs. uniprot
Match: A0A662X664_9STRA (Uncharacterized protein (Fragment) n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662X664_9STRA)

HSP 1 Score: 159 bits (401), Expect = 1.280e-42
Identity = 78/141 (55.32%), Postives = 99/141 (70.21%), Query Frame = 1
Query:   10 QFYLTEADLGKPRAAACVAKLADLNRYVGVSTASGTVTESLVSSFQVVVMIDATLEEQLRVNDLCHQKGVCYISCDARGVFAYALCDFGDAFVVSDIDGNQAASCVVSSITKDAVGLVTVMDDQRHNLVTGDVVTFNSVQG 432
            QFYLTEAD+GKPRAA  V KLA+LN YV V   SG +TE  +  F+ VV+++A L+E  R+N +CH K + +IS +ARGVF    CDFGD F+VSD DG +  SC++SSI+  +  LVTV DD RH L TGDVV+F  V G
Sbjct:  135 QFYLTEADIGKPRAAVSVKKLAELNPYVPVRCHSGGITEEFLLGFRTVVLVNAPLKEAKRINSICHAKSIAFISTEARGVFGSVFCDFGDEFLVSDRDGVEPVSCMISSISNSSPPLVTVSDDTRHGLETGDVVSFREVTG 275          
BLAST of mRNA_L-elsbetiae_contig7175.16010.1 vs. uniprot
Match: UPI001401E190 (ubiquitin-like modifier-activating enzyme 1 isoform X1 n=2 Tax=Petromyzon marinus TaxID=7757 RepID=UPI001401E190)

HSP 1 Score: 157 bits (398), Expect = 7.690e-42
Identity = 76/145 (52.41%), Postives = 100/145 (68.97%), Query Frame = 1
Query:   10 QFYLTEADLGKPRAAACVAKLADLNRYVGVSTASGTVTESLVSSFQVVVMIDATLEEQLRVNDLCHQKGVCYISCDARGVFAYALCDFGDAFVVSDIDGNQAASCVVSSITKDAVGLVTVMDDQRHNLVTGDVVTFNSVQGMTEV 444
            QFYL E DLGK RA  C  +L++LN YV VS  +G +TE  +S F VVV+ ++TLEE LRV DLCH KG+ +I+ D +G+F    CDFGD F V D  G Q  S ++S++TKDAVG+VT +D+ RH   +GD V F  +QGMTE+
Sbjct:  112 QFYLPEKDLGKNRAEVCQPQLSELNTYVHVSAYTGELTEEYLSKFLVVVLTNSTLEECLRVGDLCHSKGIKFIAADTKGLFGQLFCDFGDEFTVMDSTGEQPVSAMISAVTKDAVGVVTCLDETRHGFESGDHVIFTEIQGMTEL 256          
BLAST of mRNA_L-elsbetiae_contig7175.16010.1 vs. uniprot
Match: W2NN34_PHYPR (E1 ubiquitin-activating enzyme n=8 Tax=Phytophthora parasitica TaxID=4792 RepID=W2NN34_PHYPR)

HSP 1 Score: 156 bits (394), Expect = 1.800e-41
Identity = 76/141 (53.90%), Postives = 98/141 (69.50%), Query Frame = 1
Query:   10 QFYLTEADLGKPRAAACVAKLADLNRYVGVSTASGTVTESLVSSFQVVVMIDATLEEQLRVNDLCHQKGVCYISCDARGVFAYALCDFGDAFVVSDIDGNQAASCVVSSITKDAVGLVTVMDDQRHNLVTGDVVTFNSVQG 432
            QFYLTE D+GKPRAA  V KLA+LN YV V   SG +TE  +  F+ VV+++A L+E  R+N +CH K + +I+ +ARGVF    CDFGD F+VSD DG +  SC++SSI+  A  LVTV DD RH L TGD+V+F  V G
Sbjct:  116 QFYLTEGDMGKPRAAVSVKKLAELNPYVPVRCYSGEITEEFLLGFRAVVLVNAPLKEAKRINAICHAKSIAFITTEARGVFGSVFCDFGDEFIVSDRDGVEPVSCLISSISNSAPPLVTVNDDTRHGLETGDLVSFREVAG 256          
BLAST of mRNA_L-elsbetiae_contig7175.16010.1 vs. uniprot
Match: A0A5D6Y0E3_9STRA (E1 ubiquitin-activating enzyme n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6Y0E3_9STRA)

HSP 1 Score: 155 bits (393), Expect = 3.630e-41
Identity = 76/141 (53.90%), Postives = 95/141 (67.38%), Query Frame = 1
Query:   10 QFYLTEADLGKPRAAACVAKLADLNRYVGVSTASGTVTESLVSSFQVVVMIDATLEEQLRVNDLCHQKGVCYISCDARGVFAYALCDFGDAFVVSDIDGNQAASCVVSSITKDAVGLVTVMDDQRHNLVTGDVVTFNSVQG 432
            QFYL+E D+G PRA     KLA+LN YV V   SG ++E+ +  F+VVV+++A L E  R+N LCH  G+ ++S +ARGVF    CDFGDAFVVSD DG Q  SC+V+SI      LVTV DD RH L TGDVVTF  + G
Sbjct:  111 QFYLSERDVGAPRAQVSAKKLAELNPYVHVHAHSGDISEAFLQQFRVVVVVNAALSEAKRINALCHAHGIAFVSTEARGVFGAVFCDFGDAFVVSDRDGEQPVSCMVASIANSTPPLVTVSDDARHQLETGDVVTFREISG 251          
BLAST of mRNA_L-elsbetiae_contig7175.16010.1 vs. uniprot
Match: A0A3R7H2J5_9STRA (E1 ubiquitin-activating enzyme n=4 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A3R7H2J5_9STRA)

HSP 1 Score: 155 bits (393), Expect = 3.630e-41
Identity = 75/141 (53.19%), Postives = 98/141 (69.50%), Query Frame = 1
Query:   10 QFYLTEADLGKPRAAACVAKLADLNRYVGVSTASGTVTESLVSSFQVVVMIDATLEEQLRVNDLCHQKGVCYISCDARGVFAYALCDFGDAFVVSDIDGNQAASCVVSSITKDAVGLVTVMDDQRHNLVTGDVVTFNSVQG 432
            QFYLTEAD+GKPRA   V KLA+LN YV V   SGT+TE  +  F+ VV+++++L+E  R+N +CH K + +I+ +ARGVF    CDFGD FV+SD DG    SC++SSI+     LVTV DD RH L TGD+V+F  V G
Sbjct:  125 QFYLTEADIGKPRATVSVQKLAELNPYVPVRCHSGTITEDFLHGFRAVVLVNSSLKEAKRINAICHAKSIAFITTEARGVFGSIFCDFGDEFVISDRDGVDPVSCLISSISDSTPPLVTVSDDARHGLETGDLVSFREVPG 265          
BLAST of mRNA_L-elsbetiae_contig7175.16010.1 vs. uniprot
Match: A0A8C9RXZ4_SCLFO (Ubiquitin-like modifier activating enzyme 1 n=1 Tax=Scleropages formosus TaxID=113540 RepID=A0A8C9RXZ4_SCLFO)

HSP 1 Score: 155 bits (391), Expect = 5.740e-41
Identity = 75/145 (51.72%), Postives = 98/145 (67.59%), Query Frame = 1
Query:   10 QFYLTEADLGKPRAAACVAKLADLNRYVGVSTASGTVTESLVSSFQVVVMIDATLEEQLRVNDLCHQKGVCYISCDARGVFAYALCDFGDAFVVSDIDGNQAASCVVSSITKDAVGLVTVMDDQRHNLVTGDVVTFNSVQGMTEV 444
            QFYL E DLG+ RA     +LA+LN YV V+  +G +TE  ++ FQVV++ D+ LEEQLR+ DLCH KG+  I  D RG+F    CDFG+  +V D +G Q  S ++S ITKD+ GLVT +D+ RH   TGD VTF  VQGMTE+
Sbjct:   75 QFYLREEDLGRNRAEVSQPRLAELNSYVPVTAYTGALTEEYLTEFQVVILTDSNLEEQLRLGDLCHSKGIKLIIADTRGLFGQLFCDFGEEMIVYDTNGEQPLSAMISMITKDSQGLVTCLDEARHGFETGDYVTFTEVQGMTEL 219          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig7175.16010.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5J7N3_9PHAE1.990e-7582.76E1 ubiquitin-activating enzyme n=2 Tax=Ectocarpus ... [more]
A0A836CIM1_9STRA5.570e-5266.21Ubiquitin-activating enzyme E1 n=1 Tax=Tribonema m... [more]
UPI0008541C9A3.480e-4352.41ubiquitin-like modifier-activating enzyme 1 isofor... [more]
A0A7S3UZM0_9STRA4.680e-4354.11Hypothetical protein n=1 Tax=Aplanochytrium stocch... [more]
A0A662X664_9STRA1.280e-4255.32Uncharacterized protein (Fragment) n=2 Tax=Nothoph... [more]
UPI001401E1907.690e-4252.41ubiquitin-like modifier-activating enzyme 1 isofor... [more]
W2NN34_PHYPR1.800e-4153.90E1 ubiquitin-activating enzyme n=8 Tax=Phytophthor... [more]
A0A5D6Y0E3_9STRA3.630e-4153.90E1 ubiquitin-activating enzyme n=1 Tax=Pythium bra... [more]
A0A3R7H2J5_9STRA3.630e-4153.19E1 ubiquitin-activating enzyme n=4 Tax=Phytophthor... [more]
A0A8C9RXZ4_SCLFO5.740e-4151.72Ubiquitin-like modifier activating enzyme 1 n=1 Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig7175contigL-elsbetiae_contig7175:8..945 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score254.6
Seed ortholog evalue2.8e-65
Seed eggNOG ortholog2880.D7G2V5
Preferred nameUBA1
KEGG koko:K03178
KEGG Pathwayko04120,ko05012,map04120,map05012
Hectar predicted targeting categoryother localisation
EggNOG free text desc.ubiquitin activating enzyme activity
EggNOG OGsCOG0476@1,KOG2012@2759
EC6.2.1.45
COG Functional cat.H
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko04121,ko04147
Ec32 ortholog descriptionubiquitin-activating enzyme E1
Ec32 orthologEc-09_004750.1
Exons2
Model size444
Cds size444
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816797.7522368-CDS-L-elsbetiae_contig7175:7..3551622816797.7522368-CDS-L-elsbetiae_contig7175:7..355Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig7175 8..355 -
1691683821.077561-CDS-L-elsbetiae_contig7175:7..3551691683821.077561-CDS-L-elsbetiae_contig7175:7..355Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig7175 8..355 -
1622816797.7638228-CDS-L-elsbetiae_contig7175:849..9451622816797.7638228-CDS-L-elsbetiae_contig7175:849..945Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig7175 850..945 -
1691683821.0920987-CDS-L-elsbetiae_contig7175:849..9451691683821.0920987-CDS-L-elsbetiae_contig7175:849..945Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig7175 850..945 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig7175.16010.1prot_L-elsbetiae_contig7175.16010.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig7175 8..945 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig7175.16010.1

>prot_L-elsbetiae_contig7175.16010.1 ID=prot_L-elsbetiae_contig7175.16010.1|Name=mRNA_L-elsbetiae_contig7175.16010.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=148bp
MPWQFYLTEADLGKPRAAACVAKLADLNRYVGVSTASGTVTESLVSSFQV
VVMIDATLEEQLRVNDLCHQKGVCYISCDARGVFAYALCDFGDAFVVSDI
DGNQAASCVVSSITKDAVGLVTVMDDQRHNLVTGDVVTFNSVQGMTEV
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mRNA from alignment at L-elsbetiae_contig7175:8..945-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig7175.16010.1 ID=mRNA_L-elsbetiae_contig7175.16010.1|Name=mRNA_L-elsbetiae_contig7175.16010.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=938bp|location=Sequence derived from alignment at L-elsbetiae_contig7175:8..945- (Laminarionema elsbetiae ELsaHSoW15)
ATGCCATGGCAGTTCTACCTGACGGAGGCAGACCTGGGCAAGCCTCGGGC AGCCGCGTGTGTGGCGAAGCTGGCGGACCTCAACCGATACGTTGGGGTGA GCTAAGTGGTGGTCGGCGTATCCGTGCCTGTGTGTGTGTGTGTGTAGACA GCACACGACTGCTGCTGCGTATCCTCATCATCTCACAGCTTTAGTAGCAC GATAATGGATGTTACTTGTAGATGTATACGTAGAGTAGTAGCTGTGCAGA GGTGGGCGGGGCATTCTCTTTTTTTGTTATTGTCGTATTTGCGTCGACGT AGATGTTCTCCATCGGTTACTCCCTCCGCGCCATATGCCAAGGCTCAAAG CTTGCTCTAAATAGCTGTGACAGTTTGTTCCTCGGCTACATCGAAACCCA CACCACACATCGCCTGCAGTCACTGGCTTAATCTCTCAACACTCAGCATC GCATATCCTTACTCTCATTTCACGTACAGCAGTAGTTGCTGTGCTGCTGC ATTCGTTCCTAGGTGCCCGGAAATTTTTCTGTGACGTCTTCGTCCTTGTC TTTCTTTGCCTTGGCGTCTCTGGGCTAATCCTGTCGTCAGGTGTCGACGG CAAGCGGCACCGTGACTGAATCCTTGGTGTCCTCGTTCCAAGTGGTGGTG ATGATCGACGCGACCCTCGAGGAGCAGCTCAGGGTCAACGACCTGTGCCA CCAGAAGGGTGTGTGCTACATCTCGTGCGACGCGCGGGGCGTGTTCGCCT ACGCCTTGTGCGACTTCGGGGACGCGTTCGTGGTCTCGGACATAGACGGC AACCAGGCCGCGTCGTGCGTCGTGTCCTCCATCACAAAGGACGCGGTTGG TCTGGTGACCGTCATGGACGACCAGCGGCACAACCTTGTGACCGGGGATG TCGTCACGTTCAACTCTGTTCAGGGCATGACGGAGGTA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig7175:8..945-

>mRNA_L-elsbetiae_contig7175.16010.1 ID=mRNA_L-elsbetiae_contig7175.16010.1|Name=mRNA_L-elsbetiae_contig7175.16010.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=888bp|location=Sequence derived from alignment at L-elsbetiae_contig7175:8..945- (Laminarionema elsbetiae ELsaHSoW15)
ATGCCATGGCAGTTCTACCTGACGGAGGCAGACCTGGGCAAGCCTCGGGC
AGCCGCGTGTGTGGCGAAGCTGGCGGACCTCAACCGATACGTTGGGATGC
CATGGCAGTTCTACCTGACGGAGGCAGACCTGGGCAAGCCTCGGGCAGCC
GCGTGTGTGGCGAAGCTGGCGGACCTCAACCGATACGTTGGGGTGTCGAC
GGCAAGCGGCACCGTGACTGAATCCTTGGTGTCCTCGTTCCAAGTGGTGG
TGATGATCGACGCGACCCTCGAGGAGCAGCTCAGGGTCAACGACCTGTGC
CACCAGAAGGGTGTGTGCTACATCTCGTGCGACGCGCGGGGCGTGTTCGC
CTACGCCTTGTGCGACTTCGGGGACGCGTTCGTGGTCTCGGACATAGACG
GCAACCAGGCCGCGTCGTGCGTCGTGTCCTCCATCACAAAGGACGCGGTT
GGTCTGGTGACCGTCATGGACGACCAGCGGCACAACCTTGTGACCGGGGA
TGTCGTCACGTTCAACTCTGTTCAGGGCATGACGGAGGTAGTGTCGACGG
CAAGCGGCACCGTGACTGAATCCTTGGTGTCCTCGTTCCAAGTGGTGGTG
ATGATCGACGCGACCCTCGAGGAGCAGCTCAGGGTCAACGACCTGTGCCA
CCAGAAGGGTGTGTGCTACATCTCGTGCGACGCGCGGGGCGTGTTCGCCT
ACGCCTTGTGCGACTTCGGGGACGCGTTCGTGGTCTCGGACATAGACGGC
AACCAGGCCGCGTCGTGCGTCGTGTCCTCCATCACAAAGGACGCGGTTGG
TCTGGTGACCGTCATGGACGACCAGCGGCACAACCTTGTGACCGGGGATG
TCGTCACGTTCAACTCTGTTCAGGGCATGACGGAGGTA
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