mRNA_L-elsbetiae_contig6171.14750.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig6171.14750.1
Unique NamemRNA_L-elsbetiae_contig6171.14750.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig6171.14750.1 vs. uniprot
Match: D8LN02_ECTSI (Dynein heavy chain dynein heavy chain n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LN02_ECTSI)

HSP 1 Score: 211 bits (538), Expect = 1.810e-61
Identity = 102/103 (99.03%), Postives = 103/103 (100.00%), Query Frame = 2
Query:    2 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLRLLGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 310
            GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLR+LGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP
Sbjct: 1140 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLRILGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 1242          
BLAST of mRNA_L-elsbetiae_contig6171.14750.1 vs. uniprot
Match: A0A6H5L381_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L381_9PHAE)

HSP 1 Score: 202 bits (514), Expect = 3.050e-58
Identity = 100/105 (95.24%), Postives = 101/105 (96.19%), Query Frame = 2
Query:    2 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLR--LLGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 310
            GIDFQSMG+IFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRP LR  LLGREIDVDLN GIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP
Sbjct:  239 GIDFQSMGKIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPFLRQALLGREIDVDLNTGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 343          
BLAST of mRNA_L-elsbetiae_contig6171.14750.1 vs. uniprot
Match: A0A7S1TTM0_9STRA (Hypothetical protein (Fragment) n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1TTM0_9STRA)

HSP 1 Score: 188 bits (477), Expect = 1.290e-56
Identity = 88/103 (85.44%), Postives = 96/103 (93.20%), Query Frame = 2
Query:    2 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLRLLGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 310
            GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSA+S QIQIIQDAIK +R  ++LLGR I+V+ NAGIFVTLNPAGK YGGRS+LPDNLKALFRPVAMG+P
Sbjct:  137 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAVSTQIQIIQDAIKTRRRQIKLLGRTIEVNFNAGIFVTLNPAGKDYGGRSRLPDNLKALFRPVAMGKP 239          
BLAST of mRNA_L-elsbetiae_contig6171.14750.1 vs. uniprot
Match: A0A836CKQ4_9STRA (Dynein heavy chain, N-terminal region 2-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CKQ4_9STRA)

HSP 1 Score: 196 bits (498), Expect = 4.400e-56
Identity = 91/103 (88.35%), Postives = 98/103 (95.15%), Query Frame = 2
Query:    2 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLRLLGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 310
            GIDFQSMGRI IGL KCGAWGCFDEFNRLKEDQLSA+SQQIQ+IQDAIKA++P+LRLLGR +DVD NAGIFVTLNPAGK YGGRS+LPDNLKALFRPVAMGRP
Sbjct:  665 GIDFQSMGRILIGLTKCGAWGCFDEFNRLKEDQLSAVSQQIQVIQDAIKARQPTLRLLGRAVDVDFNAGIFVTLNPAGKDYGGRSRLPDNLKALFRPVAMGRP 767          
BLAST of mRNA_L-elsbetiae_contig6171.14750.1 vs. uniprot
Match: A0A8J2SHJ0_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SHJ0_9STRA)

HSP 1 Score: 192 bits (489), Expect = 7.250e-55
Identity = 91/103 (88.35%), Postives = 97/103 (94.17%), Query Frame = 2
Query:    2 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLRLLGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 310
            GIDF SMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQ+IQDAIKA+ P L L+GREI+VD NAGIFVTLNPAGK YGGRS++PDNLKALFRPVAMGRP
Sbjct: 2318 GIDFHSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQVIQDAIKARAPVLSLVGREINVDFNAGIFVTLNPAGKDYGGRSQIPDNLKALFRPVAMGRP 2420          
BLAST of mRNA_L-elsbetiae_contig6171.14750.1 vs. uniprot
Match: A0A2P4WW42_9STRA (Dynein heavy chain (Fragment) n=1 Tax=Phytophthora palmivora var. palmivora TaxID=611791 RepID=A0A2P4WW42_9STRA)

HSP 1 Score: 191 bits (486), Expect = 1.760e-54
Identity = 91/103 (88.35%), Postives = 95/103 (92.23%), Query Frame = 2
Query:    2 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLRLLGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 310
            GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQ+IQDAIK K  ++ LL R +DVD NAGIFVTLNPAGK YGGRSKLPDNLKALFRPVAMGRP
Sbjct:  613 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQVIQDAIKEKTGTINLLNRSVDVDFNAGIFVTLNPAGKGYGGRSKLPDNLKALFRPVAMGRP 715          
BLAST of mRNA_L-elsbetiae_contig6171.14750.1 vs. uniprot
Match: A0A8J5MA63_9STRA (Uncharacterized protein n=1 Tax=Phytophthora aleatoria TaxID=2496075 RepID=A0A8J5MA63_9STRA)

HSP 1 Score: 191 bits (486), Expect = 1.830e-54
Identity = 91/103 (88.35%), Postives = 95/103 (92.23%), Query Frame = 2
Query:    2 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLRLLGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 310
            GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQ+IQDAIK K  ++ LL R +DVD NAGIFVTLNPAGK YGGRSKLPDNLKALFRPVAMGRP
Sbjct:   40 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQVIQDAIKEKTGNINLLNRSVDVDFNAGIFVTLNPAGKGYGGRSKLPDNLKALFRPVAMGRP 142          
BLAST of mRNA_L-elsbetiae_contig6171.14750.1 vs. uniprot
Match: H3HBY2_PHYRM (Uncharacterized protein n=1 Tax=Phytophthora ramorum TaxID=164328 RepID=H3HBY2_PHYRM)

HSP 1 Score: 191 bits (486), Expect = 1.830e-54
Identity = 91/103 (88.35%), Postives = 95/103 (92.23%), Query Frame = 2
Query:    2 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLRLLGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 310
            GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQ+IQDAIK K  ++ LL R +DVD NAGIFVTLNPAGK YGGRSKLPDNLKALFRPVAMGRP
Sbjct: 1780 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQVIQDAIKEKTGTINLLNRSVDVDFNAGIFVTLNPAGKGYGGRSKLPDNLKALFRPVAMGRP 1882          
BLAST of mRNA_L-elsbetiae_contig6171.14750.1 vs. uniprot
Match: A0A662XLI3_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662XLI3_9STRA)

HSP 1 Score: 191 bits (486), Expect = 1.830e-54
Identity = 91/103 (88.35%), Postives = 96/103 (93.20%), Query Frame = 2
Query:    2 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLRLLGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 310
            GIDFQSMGRIF+GLVKCGAWGCFDEFNRLKEDQLSAISQQIQ+IQDAIK K  S++LL R +DVD NAGIFVTLNPAGK YGGRSKLPDNLKALFRPVAMGRP
Sbjct: 1256 GIDFQSMGRIFLGLVKCGAWGCFDEFNRLKEDQLSAISQQIQVIQDAIKDKTRSIQLLNRAVDVDFNAGIFVTLNPAGKGYGGRSKLPDNLKALFRPVAMGRP 1358          
BLAST of mRNA_L-elsbetiae_contig6171.14750.1 vs. uniprot
Match: A0A225WXS6_9STRA (Dynein heavy chain n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225WXS6_9STRA)

HSP 1 Score: 191 bits (486), Expect = 1.830e-54
Identity = 91/103 (88.35%), Postives = 95/103 (92.23%), Query Frame = 2
Query:    2 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQIIQDAIKAKRPSLRLLGREIDVDLNAGIFVTLNPAGKKYGGRSKLPDNLKALFRPVAMGRP 310
            GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQ+IQDAIK K  ++ LL R +DVD NAGIFVTLNPAGK YGGRSKLPDNLKALFRPVAMGRP
Sbjct: 1776 GIDFQSMGRIFIGLVKCGAWGCFDEFNRLKEDQLSAISQQIQVIQDAIKEKTGTINLLSRSVDVDFNAGIFVTLNPAGKGYGGRSKLPDNLKALFRPVAMGRP 1878          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig6171.14750.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LN02_ECTSI1.810e-6199.03Dynein heavy chain dynein heavy chain n=1 Tax=Ecto... [more]
A0A6H5L381_9PHAE3.050e-5895.24Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S1TTM0_9STRA1.290e-5685.44Hypothetical protein (Fragment) n=1 Tax=Phaeomonas... [more]
A0A836CKQ4_9STRA4.400e-5688.35Dynein heavy chain, N-terminal region 2-domain-con... [more]
A0A8J2SHJ0_9STRA7.250e-5588.35Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
A0A2P4WW42_9STRA1.760e-5488.35Dynein heavy chain (Fragment) n=1 Tax=Phytophthora... [more]
A0A8J5MA63_9STRA1.830e-5488.35Uncharacterized protein n=1 Tax=Phytophthora aleat... [more]
H3HBY2_PHYRM1.830e-5488.35Uncharacterized protein n=1 Tax=Phytophthora ramor... [more]
A0A662XLI3_9STRA1.830e-5488.35Uncharacterized protein (Fragment) n=1 Tax=Nothoph... [more]
A0A225WXS6_9STRA1.830e-5488.35Dynein heavy chain n=1 Tax=Phytophthora megakarya ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig6171contigL-elsbetiae_contig6171:5565..7763 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons3
Model size314
Cds size312
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig6171.14750.1prot_L-elsbetiae_contig6171.14750.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig6171 5565..7762 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816676.3772693-CDS-L-elsbetiae_contig6171:5564..56131622816676.3772693-CDS-L-elsbetiae_contig6171:5564..5613Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig6171 5565..5613 +
1691683731.9903708-CDS-L-elsbetiae_contig6171:5564..56131691683731.9903708-CDS-L-elsbetiae_contig6171:5564..5613Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig6171 5565..5613 +
1622816676.390015-CDS-L-elsbetiae_contig6171:6255..63741622816676.390015-CDS-L-elsbetiae_contig6171:6255..6374Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig6171 6256..6374 +
1691683732.0084147-CDS-L-elsbetiae_contig6171:6255..63741691683732.0084147-CDS-L-elsbetiae_contig6171:6255..6374Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig6171 6256..6374 +
1622816676.4006531-CDS-L-elsbetiae_contig6171:7618..77621622816676.4006531-CDS-L-elsbetiae_contig6171:7618..7762Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig6171 7619..7762 +
1691683732.0228627-CDS-L-elsbetiae_contig6171:7618..77621691683732.0228627-CDS-L-elsbetiae_contig6171:7618..7762Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig6171 7619..7762 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig6171.14750.1

>prot_L-elsbetiae_contig6171.14750.1 ID=prot_L-elsbetiae_contig6171.14750.1|Name=mRNA_L-elsbetiae_contig6171.14750.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=104bp
RDRLSEHGQDLYRPCQVRCLGLLRRVQPPQGGPAVGHLAADPDHPGRDQG
EAAVAEASRPRNRRGPERRHLRDPQPGRQEIRRPQQAPGQPQGPLPTRGD
GATG
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mRNA from alignment at L-elsbetiae_contig6171:5565..7763+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig6171.14750.1 ID=mRNA_L-elsbetiae_contig6171.14750.1|Name=mRNA_L-elsbetiae_contig6171.14750.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=2199bp|location=Sequence derived from alignment at L-elsbetiae_contig6171:5565..7763+ (Laminarionema elsbetiae ELsaHSoW15)
AGGGATCGACTTTCAGAGCATGGGCAGGATCTTTATCGGCCTTGTCAAGG TATGATAGATAGGCCACGTTTCGCGGTGCGTTTGCCCGACCCCAAACACT AATGTTAGTTTTCTACGCAACACTGCTTGTTTAAAATAAAACAAAGAATC AAAGATGGAACCTAACCCATACTAGAGCAAAGACTCGATATGGGGTTCAA ACGGTATTCCATACAGAGACGTAATATTTGGCGAAGCGAGCAGAAAAACA ACTTCAAATATCAGAAGAGTTCAACATCTCCCCTGCAAAGATTTGCGAGG TCGGCCAACAAGGATTTGCTTGTTTGTTTCTGTTGCGTGGTGAGCGACTG GAGAAGACAGATTACGTGCTCTGGAAGGATCGATGTCAACGCTTGAACGG GAGAAAAGTTCTGCCAGTGTTGGGGTTTCAACTTTTGTTTTTTTATCTCC CCTCGCTAAATCGTGGTTTCGACACCCAAACTCGTGCTAGAAAATATCGA CAAAGGTTGTACGTTTTACTCAACTTTGTCCGTTTCGAATCCCGGGGTAT CGCTCTGGCGACTGCAGGCGGAGCACTGCTTCAAAATCTCACCCACTTCT GAATCACGATACCAAACGTATGCCGCCTATTCGAAAGACCGCTCCGAAGT GATTTTAAGTGTCAATGTTCACAACTCAACGAACCGATCAGTGCGGTGCT TGGGGCTGCTTCGACGAGTTCAACCGCCTCAAGGAGGACCAGCTGTCGGC CATCTCGCAGCAGATCCAGATCATCCAGGACGCGATCAAGGCGAAGCGGC CGTCGCTGAGGTGTGATGACTATTTTGAGGTGGATACTTTTTGTGAAGGT ACTGGTGTGGGGTGAGCTTCGGGGGATACACAAGCGGGTGGGTTGATGCT ACATGCGCATATGACCATTTGTTTCATGTGACTCTTTAAGAGTAACTATG CGGTACCTCGTAGTTACACACGTATCAGCATAGACTACCCACCAACATCA CAAACCACGAATAAGCCGAGATCGGGCTATCCTACTTCTAGGAGAAGAAC ATGAAGTCTCCGAGCTGATATCAGAGGCTTTCTTCGAGCGCGTTGACAAG GCCTTGAGTCATCATGGTTGCGAAGCACGGCCACGCTAGTTCGCCACTTG AGGCGTTTGGCGTTGCACCATAAAAGGCAACGCATGCACAGGAGTGGAAC GCAAATAATGAGGTTACAGCTTTCCTCGCGAACGACCCAAATGCACCGTA TTTAGTCATGCAAGCCATCGCACCCCCCCCGCACCGATTTTCGAGCTCGA CTACTTACTGCTTAAACATAGAGGCTAGCCACACCAAGCGGCACGTTATT TGGCAGCTCTCGACGTGATCGTCCCAGTGCCTCCTTTTTCGGTGGCGCCG GCACCCCCCTTCGTTAAGGCGTAATCGAGCTCTGAAAATTCAGTACGGGG GGGATGCGATGGCTCGCATGATAATGGTACTCTAACGCAAATGATAAGTA TCCTTTGTACAATGCAACGACGGGTTTCGATGCGCGAACGACGGAAAAGT CTGCCTCCGACGTCTCAAGAATGATTTTTTGTTTCCTTCTTCACATTTAT TTCCAGTGCTCGGTGGGCACCTTGCCACGAGGGCATCTTGACAGAGTACT GAACCGTGGTTGGTCACAACAGAAGGGGTCGTTCAATTCCCCGAATGCCC CACTCCCCCCTTCAGCGGTGCTTGCCTATGTAAGGCTTTTTGTCGCAAGA AGGGCTCAGCCATCCTTTTCCCTTGTCGATGGTGAGGTCGAAGCTGCTTG TACTGACATAAAGCCTAAGCTTGGTTGGTTGGACTAAATGGGACAGTGAG CAGAGGCTTATATAATGAGTGCCTGTGAAAATCGCGCTCGTTTGTACATA CACCTTATAGAAAGCAGCTTGGGTTGGCGCTGAGTTGGCCAAAAGGTGGA AAAGGAAGGAGAAGAGCGGCGGTGTTTCGAAAGAGTCCCGAACTGATAAC ATATTTAGTAAATAGTCTTCCAACACTGCCGTCCTTCCCTGGAAATTGAA ACAGGCTTCTCGGCCGAGAAATCGACGTGGACCTGAACGCCGGCATCTTC GTGACCCTCAACCCGGCCGGCAAGAAATACGGAGGCCGCAGCAAGCTCCC GGACAACCTCAAGGCCCTCTTCCGACCCGTGGCGATGGGGCGACCGGAC
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Coding sequence (CDS) from alignment at L-elsbetiae_contig6171:5565..7763+

>mRNA_L-elsbetiae_contig6171.14750.1 ID=mRNA_L-elsbetiae_contig6171.14750.1|Name=mRNA_L-elsbetiae_contig6171.14750.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=624bp|location=Sequence derived from alignment at L-elsbetiae_contig6171:5565..7763+ (Laminarionema elsbetiae ELsaHSoW15)
AGGGATCGACTTTCAGAGCATGGGCAGGATCTTTATCGGCCTTGTCAAGA
GGGATCGACTTTCAGAGCATGGGCAGGATCTTTATCGGCCTTGTCAAGTG
CGGTGCTTGGGGCTGCTTCGACGAGTTCAACCGCCTCAAGGAGGACCAGC
TGTCGGCCATCTCGCAGCAGATCCAGATCATCCAGGACGCGATCAAGGCG
AAGCGGCCGTCGCTGAGTGCGGTGCTTGGGGCTGCTTCGACGAGTTCAAC
CGCCTCAAGGAGGACCAGCTGTCGGCCATCTCGCAGCAGATCCAGATCAT
CCAGGACGCGATCAAGGCGAAGCGGCCGTCGCTGAGGCTTCTCGGCCGAG
AAATCGACGTGGACCTGAACGCCGGCATCTTCGTGACCCTCAACCCGGCC
GGCAAGAAATACGGAGGCCGCAGCAAGCTCCCGGACAACCTCAAGGCCCT
CTTCCGACCCGTGGCGATGGGGCGACCGGAGCTTCTCGGCCGAGAAATCG
ACGTGGACCTGAACGCCGGCATCTTCGTGACCCTCAACCCGGCCGGCAAG
AAATACGGAGGCCGCAGCAAGCTCCCGGACAACCTCAAGGCCCTCTTCCG
ACCCGTGGCGATGGGGCGACCGGA
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