mRNA_L-elsbetiae_contig5901.14374.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig5901.14374.1
Unique NamemRNA_L-elsbetiae_contig5901.14374.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig5901.14374.1 vs. uniprot
Match: D7FIT0_ECTSI (Chain A, Crystal Structure Of Human Phosphohistidine Phosphatase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FIT0_ECTSI)

HSP 1 Score: 212 bits (540), Expect = 5.890e-68
Identity = 103/118 (87.29%), Postives = 109/118 (92.37%), Query Frame = 1
Query:  145 GDFPFVTLDEDAVQKFAEIRVRWGG-DESSEPTILIRGLKSAEYHVEAAEPTMQRLREAGIDATITGGGRVKHSSKQRSVFIYGYSMQFGPANLPRVADLCRKQFGPEYEVTWTEGGY 495
            G FP VTLD DAVQKFAEIRVRWGG DESSEPTILIRGL+SA YH+EAAEPTM RLREAG DATITGGGR+KHSSK+RS+FIYGYSMQFGPANLPRVADLCRKQF  +YEVTWTEGGY
Sbjct:   41 GGFPSVTLDADAVQKFAEIRVRWGGADESSEPTILIRGLQSATYHIEAAEPTMARLREAGADATITGGGRIKHSSKRRSIFIYGYSMQFGPANLPRVADLCRKQFPSDYEVTWTEGGY 158          
BLAST of mRNA_L-elsbetiae_contig5901.14374.1 vs. uniprot
Match: A0A6H5JGZ6_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JGZ6_9PHAE)

HSP 1 Score: 211 bits (538), Expect = 4.250e-66
Identity = 101/116 (87.07%), Postives = 109/116 (93.97%), Query Frame = 1
Query:  151 FPFVTLDEDAVQKFAEIRVRWGG-DESSEPTILIRGLKSAEYHVEAAEPTMQRLREAGIDATITGGGRVKHSSKQRSVFIYGYSMQFGPANLPRVADLCRKQFGPEYEVTWTEGGY 495
            FP VTLD DAVQKFAEI+VRWGG DESSEPTILIRGLKSA+YH+EAAEPTM RLREAG DAT+TGGGR+KHSSK+RS+FIYGYSMQFGPANLPRVADLCRKQF  +YEVTWTEGGY
Sbjct:  155 FPSVTLDADAVQKFAEIQVRWGGTDESSEPTILIRGLKSAKYHIEAAEPTMARLREAGADATVTGGGRIKHSSKRRSIFIYGYSMQFGPANLPRVADLCRKQFPSDYEVTWTEGGY 270          
BLAST of mRNA_L-elsbetiae_contig5901.14374.1 vs. uniprot
Match: A0A7S1HIJ1_HEMAN (Hypothetical protein n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1HIJ1_HEMAN)

HSP 1 Score: 88.2 bits (217), Expect = 3.180e-19
Identity = 47/129 (36.43%), Postives = 75/129 (58.14%), Query Frame = 1
Query:  112 AVSDGEASSSR-GDFPFVTLDEDAVQKFAEIRVRWGGDESSEPTILIRGLKSAEYHVEAAEPTMQRLREAGIDATITGGGRVKHSSKQRSVFIYGYSMQFGPANLPRVADLCRKQFGPEYEVTWTEGGY 495
            +V++GE  SS   DF  V +      K+  I  +   D       L+RG + +EYHV AA PT+++LR+ G+ A + GGGR+KH+ +Q+++ ++G S  FG  +      +C+ +F P Y+VTW  GGY
Sbjct:   21 SVAEGECESSTLDDFESVDISPSGTFKYVLIEAQ---DPQGNTKHLVRGYRDSEYHVYAARPTVEKLRDMGMSARVLGGGRLKHAPQQKTLHLWGKSHSFGREDKSVTQSVCQ-EFYPGYKVTWENGGY 145          
BLAST of mRNA_L-elsbetiae_contig5901.14374.1 vs. uniprot
Match: C3YTP4_BRAFL (14 kDa phosphohistidine phosphatase (Fragment) n=3 Tax=Branchiostoma TaxID=7737 RepID=C3YTP4_BRAFL)

HSP 1 Score: 77.8 bits (190), Expect = 1.470e-15
Identity = 40/114 (35.09%), Postives = 59/114 (51.75%), Query Frame = 1
Query:  154 PFVTLDEDAVQKFAEIRVRWGGDESSEPTILIRGLKSAEYHVEAAEPTMQRLREAGIDATITGGGRVKHSSKQRSVFIYGYSMQFGPANLPRVADLCRKQFGPEYEVTWTEGGY 495
            P V +D D   K+  IR    GD S +   ++RG   AEYH +  +     +   G D    GGGR++HSS QR++ +YGYSM FG A+     +  +K+F     +T+   GY
Sbjct:    2 PDVEIDPDGKFKYILIRCHGPGDVSKD---IVRGFAWAEYHADIYDRVAPGIEALGFDCECIGGGRIQHSSSQRTILVYGYSMGFGQADHSITVEKLKKKFPDYSSITFNNEGY 112          
BLAST of mRNA_L-elsbetiae_contig5901.14374.1 vs. uniprot
Match: Q3B8E1_XENLA (14 kDa phosphohistidine phosphatase n=3 Tax=Xenopus TaxID=8353 RepID=Q3B8E1_XENLA)

HSP 1 Score: 75.5 bits (184), Expect = 1.450e-14
Identity = 40/116 (34.48%), Postives = 64/116 (55.17%), Query Frame = 1
Query:  154 PFVTLDEDAVQKFAEIRV--RWGGDESSEPTILIRGLKSAEYHVEAAEPTMQRLREAGIDATITGGGRVKHSSKQRSVFIYGYSMQFGPANLPRVADLCRKQFGPEYEVTWTEGGY 495
            P V +D D V K+  IRV  + G +E  +   ++RG   AEYH +  +     + +   D    GGGR++HSS+ + + IYGYS+ FG A      +  + ++ P+Y+VTW + GY
Sbjct:   10 PEVDIDPDGVFKYVLIRVHVKEGSEEYKD---IVRGYGWAEYHADIYDKVAADIEKGVYDCECLGGGRIRHSSQDKKIHIYGYSLGFGRARHAVTMEKVKDKY-PDYKVTWADEGY 121          
BLAST of mRNA_L-elsbetiae_contig5901.14374.1 vs. uniprot
Match: G5E1A4_9PIPI (14 kDa phosphohistidine phosphatase (Fragment) n=1 Tax=Hymenochirus curtipes TaxID=8362 RepID=G5E1A4_9PIPI)

HSP 1 Score: 74.3 bits (181), Expect = 2.850e-14
Identity = 40/111 (36.04%), Postives = 63/111 (56.76%), Query Frame = 1
Query:  166 LDEDAVQKFAEIRV--RWGGDESSEPTILIRGLKSAEYHVEAAEPTMQRLREAGIDATITGGGRVKHSSKQRSVFIYGYSMQFGPANLPRVADLCRKQFGPEYEVTWTEGG 492
            +D D V K+  IRV  + G ++S +   ++RG   AEYH +  +     + +   D    GGGR++HSSK + + IYGYSM FG A      +  + ++ P+YEVTW++ G
Sbjct:    1 IDPDGVFKYVLIRVHVKEGSEDSRD---IVRGYGWAEYHADIYDKASADIEKDVYDCECLGGGRIRHSSKDKKIHIYGYSMGFGQARHAVAMEKIKAKY-PDYEVTWSDEG 107          
BLAST of mRNA_L-elsbetiae_contig5901.14374.1 vs. uniprot
Match: A0A0N5CR84_THECL (Uncharacterized protein n=1 Tax=Thelazia callipaeda TaxID=103827 RepID=A0A0N5CR84_THECL)

HSP 1 Score: 74.7 bits (182), Expect = 1.460e-13
Identity = 36/116 (31.03%), Postives = 66/116 (56.90%), Query Frame = 1
Query:  148 DFPFVTLDEDAVQKFAEIRVRWGGDESSEPTILIRGLKSAEYHVEAAEPTMQRLREAGIDATITGGGRVKHSSKQRSVFIYGYSMQFGPANLPRVADLCRKQFGPEYEVTWTEGGY 495
            D P V +D   V K+  +RV      +S+  +++RG  S ++H    + T + +  +       GGGR++H S++R +F+YGYS ++GPA       L +K++ P+Y++T++  GY
Sbjct:   83 DIPDVNIDPSGVFKYIVVRVT--SKSTSKEKLIVRGFTSCKWHKNILQLTEKEIGNS-FSLKCVGGGRIRHESEKRKLFVYGYSQRYGPAKHELTVSLLKKRY-PDYDITFSYEGY 194          
BLAST of mRNA_L-elsbetiae_contig5901.14374.1 vs. uniprot
Match: UPI001B8880E5 (14 kDa phosphohistidine phosphatase-like n=1 Tax=Gigantopelta aegis TaxID=1735272 RepID=UPI001B8880E5)

HSP 1 Score: 70.5 bits (171), Expect = 2.660e-12
Identity = 35/103 (33.98%), Postives = 56/103 (54.37%), Query Frame = 1
Query:  154 PFVTLDEDAVQKFAEIRVRWGGDESSEPTILIRGLKSAEYHVEAAEPTMQRLREAGIDATITGGGRVKHSSKQRSVFIYGYSMQFGPANLPRVADLCRKQFGP 462
            P V +D + V K+  I ++   D+      ++RG + AEYH +  +    RL E G+     GGGR++H S  ++++IYGYS+ FG AN     D+  K+  P
Sbjct:   47 PEVDIDANGVFKYVLIELK---DKQGLTKTIVRGYEWAEYHADILDRVAPRLCEKGLTYDCVGGGRIRHESNTKTLYIYGYSVGFGQANHRITVDIFEKELSP 146          
BLAST of mRNA_L-elsbetiae_contig5901.14374.1 vs. uniprot
Match: A0A2A2L7D2_9BILA (Uncharacterized protein n=1 Tax=Diploscapter pachys TaxID=2018661 RepID=A0A2A2L7D2_9BILA)

HSP 1 Score: 63.5 bits (153), Expect = 2.770e-9
Identity = 36/116 (31.03%), Postives = 57/116 (49.14%), Query Frame = 1
Query:  148 DFPFVTLDEDAVQKFAEIRVRWGGDESSEPTILIRGLKSAEYHVEAAEPTMQRLREAGIDATITGGGRVKHSSKQRSVFIYGYSMQFGPANLPRVADLCRKQFGPEYEVTWTEGGY 495
            D P V +D   V  F     +     +SE   ++RG  S  +H    E T ++     +     GGGR+KH S+ + +F+YGYS  FG A+      + +K + P+Y VT++  GY
Sbjct:   95 DVPDVDIDPSGVYNF---HFQVTDRSNSEEKHIVRGYASCHFHANILEETKKKT-PRDLKLKCVGGGRIKHDSEIKDLFVYGYSQGFGQADHTIAVAILKKAY-PDYSVTYSNEGY 205          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig5901.14374.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 9
Match NameE-valueIdentityDescription
D7FIT0_ECTSI5.890e-6887.29Chain A, Crystal Structure Of Human Phosphohistidi... [more]
A0A6H5JGZ6_9PHAE4.250e-6687.07Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S1HIJ1_HEMAN3.180e-1936.43Hypothetical protein n=1 Tax=Hemiselmis andersenii... [more]
C3YTP4_BRAFL1.470e-1535.0914 kDa phosphohistidine phosphatase (Fragment) n=3... [more]
Q3B8E1_XENLA1.450e-1434.4814 kDa phosphohistidine phosphatase n=3 Tax=Xenopu... [more]
G5E1A4_9PIPI2.850e-1436.0414 kDa phosphohistidine phosphatase (Fragment) n=1... [more]
A0A0N5CR84_THECL1.460e-1331.03Uncharacterized protein n=1 Tax=Thelazia callipaed... [more]
UPI001B8880E52.660e-1233.9814 kDa phosphohistidine phosphatase-like n=1 Tax=G... [more]
A0A2A2L7D2_9BILA2.770e-931.03Uncharacterized protein n=1 Tax=Diploscapter pachy... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig5901contigL-elsbetiae_contig5901:5936..7028 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeMammalia
Seed ortholog score89.0
Seed ortholog evalue2.3e-15
Seed eggNOG ortholog9978.XP_004593494.1
Preferred namePHPT1
KEGG koko:K01112
Hectar predicted targeting categoryother localisation
GOsGO:0002682,GO:0002683,GO:0002791,GO:0002793,GO:0003674,GO:0003824,GO:0004721,GO:0005246,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006464,GO:0006470,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0008200,GO:0009966,GO:0009968,GO:0009987,GO:0010646,GO:0010647,GO:0010648,GO:0010817,GO:0016247,GO:0016248,GO:0016311,GO:0016787,GO:0016788,GO:0016791,GO:0019538,GO:0019855,GO:0023051,GO:0023056,GO:0023057,GO:0032024,GO:0032879,GO:0032880,GO:0032956,GO:0032970,GO:0033043,GO:0035774,GO:0035971,GO:0036211,GO:0040012,GO:0040017,GO:0042578,GO:0043086,GO:0043170,GO:0043412,GO:0044092,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044325,GO:0044424,GO:0044444,GO:0044464,GO:0046883,GO:0046887,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0048585,GO:0050708,GO:0050714,GO:0050776,GO:0050789,GO:0050790,GO:0050794,GO:0050796,GO:0050854,GO:0050856,GO:0050858,GO:0050860,GO:0051046,GO:0051047,GO:0051049,GO:0051050,GO:0051128,GO:0051222,GO:0051223,GO:0051270,GO:0051272,GO:0051338,GO:0051339,GO:0051348,GO:0051350,GO:0051493,GO:0060341,GO:0061178,GO:0065007,GO:0065008,GO:0065009,GO:0070201,GO:0071704,GO:0090087,GO:0090276,GO:0090277,GO:0098772,GO:0099106,GO:0101006,GO:0140096,GO:1901564,GO:1903530,GO:1903532,GO:1904951,GO:2000145,GO:2000147,GO:2000249,GO:2000983,GO:2000984
EggNOG free text desc.peptidyl-histidine dephosphorylation
EggNOG OGs2CUCZ@1,2S4DR@2759,35Q7X@314146,3A611@33154,3BT9Q@33208,3D9EE@33213,3JGWD@40674,48EJ2@7711,49BJS@7742
EC3.9.1.3
COG Functional cat.S
Best tax levelEuarchontoglires
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000
Ec32 ortholog descriptionJanus
Ec32 orthologEc-20_003030.1
Exons3
Model size498
Cds size498
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig5901.14374.1prot_L-elsbetiae_contig5901.14374.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig5901 5936..7028 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816638.3838027-CDS-L-elsbetiae_contig5901:5935..60411622816638.3838027-CDS-L-elsbetiae_contig5901:5935..6041Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5901 5936..6041 -
1691683703.64455-CDS-L-elsbetiae_contig5901:5935..60411691683703.64455-CDS-L-elsbetiae_contig5901:5935..6041Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5901 5936..6041 -
1622816638.393996-CDS-L-elsbetiae_contig5901:6432..66101622816638.393996-CDS-L-elsbetiae_contig5901:6432..6610Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5901 6433..6610 -
1691683703.659178-CDS-L-elsbetiae_contig5901:6432..66101691683703.659178-CDS-L-elsbetiae_contig5901:6432..6610Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5901 6433..6610 -
1622816638.4056327-CDS-L-elsbetiae_contig5901:6814..70281622816638.4056327-CDS-L-elsbetiae_contig5901:6814..7028Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5901 6815..7028 -
1691683703.6708632-CDS-L-elsbetiae_contig5901:6814..70281691683703.6708632-CDS-L-elsbetiae_contig5901:6814..7028Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5901 6815..7028 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig5901.14374.1

>prot_L-elsbetiae_contig5901.14374.1 ID=prot_L-elsbetiae_contig5901.14374.1|Name=mRNA_L-elsbetiae_contig5901.14374.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=166bp
MSGRVGGGGGGGGGGKGEGSSASRETNPGSAAAPREGAVSDGEASSSRGD
FPFVTLDEDAVQKFAEIRVRWGGDESSEPTILIRGLKSAEYHVEAAEPTM
QRLREAGIDATITGGGRVKHSSKQRSVFIYGYSMQFGPANLPRVADLCRK
QFGPEYEVTWTEGGY*
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mRNA from alignment at L-elsbetiae_contig5901:5936..7028-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig5901.14374.1 ID=mRNA_L-elsbetiae_contig5901.14374.1|Name=mRNA_L-elsbetiae_contig5901.14374.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1093bp|location=Sequence derived from alignment at L-elsbetiae_contig5901:5936..7028- (Laminarionema elsbetiae ELsaHSoW15)
ATGAGCGGGCGTGTCGGTGGTGGCGGCGGCGGCGGGGGGGGGGGCAAAGG GGAGGGTAGTTCGGCCTCCCGAGAGACCAACCCAGGATCTGCCGCCGCCC CCCGAGAGGGTGCGGTGAGCGACGGAGAGGCGAGTAGTAGTAGAGGCGAC TTCCCGTTTGTTACGCTTGACGAAGATGCGGTGCAGAAGTTCGCCGAGAT CAGGGTTCGCTGGGGTGAGTGGTACGAATCTATCTGCTGCCGCCCCCGTC CTCGTACGCGAGTTGTACACGCATGGTGTGGCGAAGAAGGCGTATCGTAG CGGGCGGAAAAACAAACCGATGCCTGCTGCAGTTCTATGCATACCAACGA CACCGCCAAGGCGGAAGTAGCACGCGGTAGATAAACGACGATCGAATGTC TCGTTGATGTGGACACAGGCGGCGACGAGAGCTCTGAGCCGACCATACTC ATCAGGGGGTTGAAGTCCGCGGAGTATCATGTCGAAGCCGCAGAACCAAC CATGCAGCGCTTGCGCGAAGCGGGGATCGATGCGACTATTACGGGCGGCG GCCGCGTCAAGCACAGCAGCAAGCAGCGAAGCGTTTTTATCTACGGGTGA GCTGCACGTTATGGCGACATCAAGTGTGGCCCCGAATGCCCACTGTCACG AACGGCCGCCGTGGGTCGGTGGGTGTTTTTCTTGCGTGCGGATCTGTCAA ATGGTTCTGTCTCTTGAGAGATGTGTTCGGCTTCATTCTTGAGCACCCAG CCCCTATGTCCGTAGACAGTTTTGCATGCAAAAAAATAGGTATCTGTTTT TATTCGAAGTGCAGAGACGAGTATGACTGGAGCAGACAGCTTGCTGCGCC CTAGGACGCTCCCTTCTCTGGGGTGGCGGTACATGACTGAGGCAGTGAGC TCGTAAGGTCTCACGACACTCACCCTTTCATGACCTTTCGTTTTTCTTCG ATCGTGAAAATCCCTCGGATTCCCCTTCCCAACACAGGTACAGCATGCAG TTTGGCCCCGCGAATCTCCCGCGCGTGGCTGATCTTTGTCGCAAGCAGTT TGGCCCGGAGTATGAGGTCACGTGGACCGAAGGCGGCTACTAA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig5901:5936..7028-

>mRNA_L-elsbetiae_contig5901.14374.1 ID=mRNA_L-elsbetiae_contig5901.14374.1|Name=mRNA_L-elsbetiae_contig5901.14374.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=996bp|location=Sequence derived from alignment at L-elsbetiae_contig5901:5936..7028- (Laminarionema elsbetiae ELsaHSoW15)
ATGAGCGGGCGTGTCGGTGGTGGCGGCGGCGGCGGGGGGGGGGGCAAAGG
GGAGGGTAGTTCGGCCTCCCGAGAGACCAACCCAGGATCTGCCGCCGCCC
CCCGAGAGGGTGCGGTGAGCGACGGAGAGGCGAGTAGTAGTAGAGGCGAC
TTCCCGTTTGTTACGCTTGACGAAGATGCGGTGCAGAAGTTCGCCGAGAT
CAGGGTTCGCTGGGATGAGCGGGCGTGTCGGTGGTGGCGGCGGCGGCGGG
GGGGGGGGCAAAGGGGAGGGTAGTTCGGCCTCCCGAGAGACCAACCCAGG
ATCTGCCGCCGCCCCCCGAGAGGGTGCGGTGAGCGACGGAGAGGCGAGTA
GTAGTAGAGGCGACTTCCCGTTTGTTACGCTTGACGAAGATGCGGTGCAG
AAGTTCGCCGAGATCAGGGTTCGCTGGGGCGGCGACGAGAGCTCTGAGCC
GACCATACTCATCAGGGGGTTGAAGTCCGCGGAGTATCATGTCGAAGCCG
CAGAACCAACCATGCAGCGCTTGCGCGAAGCGGGGATCGATGCGACTATT
ACGGGCGGCGGCCGCGTCAAGCACAGCAGCAAGCAGCGAAGCGTTTTTAT
CTACGGGCGGCGACGAGAGCTCTGAGCCGACCATACTCATCAGGGGGTTG
AAGTCCGCGGAGTATCATGTCGAAGCCGCAGAACCAACCATGCAGCGCTT
GCGCGAAGCGGGGATCGATGCGACTATTACGGGCGGCGGCCGCGTCAAGC
ACAGCAGCAAGCAGCGAAGCGTTTTTATCTACGGGTACAGCATGCAGTTT
GGCCCCGCGAATCTCCCGCGCGTGGCTGATCTTTGTCGCAAGCAGTTTGG
CCCGGAGTATGAGGTCACGTGGACCGAAGGCGGCTACTAAGTACAGCATG
CAGTTTGGCCCCGCGAATCTCCCGCGCGTGGCTGATCTTTGTCGCAAGCA
GTTTGGCCCGGAGTATGAGGTCACGTGGACCGAAGGCGGCTACTAA
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