mRNA_L-elsbetiae_contig5460.13719.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig5460.13719.1
Unique NamemRNA_L-elsbetiae_contig5460.13719.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
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BLAST of mRNA_L-elsbetiae_contig5460.13719.1 vs. uniprot
Match: D7G2I3_ECTSI (PH domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G2I3_ECTSI)

HSP 1 Score: 292 bits (747), Expect = 2.360e-98
Identity = 145/156 (92.95%), Postives = 154/156 (98.72%), Query Frame = 1 
Query:    1 TMDRSQKQKAGTLSVMKYKVTMSQAATNARESTRRRKDSVTSRAMRRVKRPVHQKWTEALDWKKRTCLLTGESLSLFSDPKDTEPSQIVYLRGANAEPELGQAFKESAFRVETNKWKKGDELVDGTERRTFTFLAESKDEKELWLYQIRCGTKTCS 468
            T+D+SQKQKAGTLSVMKYKVTMSQAATNARESTRRRK+SVTSRAM+RVKRPV QKWTEALDWKKR+CLLTGESLSLFSDPKDTEPSQIVYL+GA AEPELGQAFK+SAFRVETNKWKKGDE+VDGTERRTFTFLAESKD+KELWLYQIRCGTKTCS
Sbjct:   90 TIDKSQKQKAGTLSVMKYKVTMSQAATNARESTRRRKNSVTSRAMQRVKRPVQQKWTEALDWKKRSCLLTGESLSLFSDPKDTEPSQIVYLKGATAEPELGQAFKDSAFRVETNKWKKGDEVVDGTERRTFTFLAESKDDKELWLYQIRCGTKTCS 245
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BLAST of mRNA_L-elsbetiae_contig5460.13719.1 vs. uniprot
Match: A0A6H5JJ45_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JJ45_9PHAE)

HSP 1 Score: 265 bits (676), Expect = 9.880e-82
Identity = 132/149 (88.59%), Postives = 143/149 (95.97%), Query Frame = 1 
Query:    1 TMDRSQKQKAGTLSVMKYKVTMSQAATNARESTRRRKDSVTSRAMRRVKRPVHQKWTEALDWKKRTCLLTGESLSLFSDPKDTEPSQIVYLRGANAEPELGQAFKESAFRVETNKWKKGDELVDGTERRTFTFLAESKDEKELWLYQIR 447
            T+D+S K K GTLSVMK+KVTMSQAATNARESTRRRK+SVTSRAMR+VKRPV QKWTEA+DWKKR+C+LTGESLSLFSDPKDTEPSQIVYL GA AEPELGQAFKESAFRVETNKWKKGD+LVDGTERRTFTFLAESKD++ELWLYQIR
Sbjct:  392 TIDKSHKHKEGTLSVMKHKVTMSQAATNARESTRRRKNSVTSRAMRKVKRPVQQKWTEAVDWKKRSCILTGESLSLFSDPKDTEPSQIVYLTGATAEPELGQAFKESAFRVETNKWKKGDDLVDGTERRTFTFLAESKDDRELWLYQIR 540
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig5460.13719.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
D7G2I3_ECTSI2.360e-9892.95PH domain-containing protein n=1 Tax=Ectocarpus si... [more]
A0A6H5JJ45_9PHAE9.880e-8288.59Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig5460contigL-elsbetiae_contig5460:113..5934 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionPleckstrin homology-like domain
Ec32 orthologEc-12_001630.1
Exons4
Model size471
Cds size468
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig5460.13719.1prot_L-elsbetiae_contig5460.13719.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig5460 116..5934 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816572.3780797-UTR-L-elsbetiae_contig5460:112..1151622816572.3780797-UTR-L-elsbetiae_contig5460:112..115Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5460 113..115 +
1691683656.04969-UTR-L-elsbetiae_contig5460:112..1151691683656.04969-UTR-L-elsbetiae_contig5460:112..115Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5460 113..115 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816572.38856-CDS-L-elsbetiae_contig5460:115..1841622816572.38856-CDS-L-elsbetiae_contig5460:115..184Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5460 116..184 +
1691683656.062606-CDS-L-elsbetiae_contig5460:115..1841691683656.062606-CDS-L-elsbetiae_contig5460:115..184Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5460 116..184 +
1622816572.3983982-CDS-L-elsbetiae_contig5460:1229..14631622816572.3983982-CDS-L-elsbetiae_contig5460:1229..1463Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5460 1230..1463 +
1691683656.072152-CDS-L-elsbetiae_contig5460:1229..14631691683656.072152-CDS-L-elsbetiae_contig5460:1229..1463Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5460 1230..1463 +
1622816572.4124267-CDS-L-elsbetiae_contig5460:2265..24051622816572.4124267-CDS-L-elsbetiae_contig5460:2265..2405Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5460 2266..2405 +
1691683656.0811536-CDS-L-elsbetiae_contig5460:2265..24051691683656.0811536-CDS-L-elsbetiae_contig5460:2265..2405Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5460 2266..2405 +
1622816572.4249406-CDS-L-elsbetiae_contig5460:5909..59341622816572.4249406-CDS-L-elsbetiae_contig5460:5909..5934Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5460 5910..5934 +
1691683656.0900562-CDS-L-elsbetiae_contig5460:5909..59341691683656.0900562-CDS-L-elsbetiae_contig5460:5909..5934Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5460 5910..5934 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig5460.13719.1

>prot_L-elsbetiae_contig5460.13719.1 ID=prot_L-elsbetiae_contig5460.13719.1|Name=mRNA_L-elsbetiae_contig5460.13719.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=156bp
MDRSQKQKAGTLSVMKYKVTMSQAATNARESTRRRKDSVTSRAMRRVKRP
VHQKWTEALDWKKRTCLLTGESLSLFSDPKDTEPSQIVYLRGANAEPELG
QAFKESAFRVETNKWKKGDELVDGTERRTFTFLAESKDEKELWLYQIRCG
TKTCS*
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mRNA from alignment at L-elsbetiae_contig5460:113..5934+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig5460.13719.1 ID=mRNA_L-elsbetiae_contig5460.13719.1|Name=mRNA_L-elsbetiae_contig5460.13719.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=5822bp|location=Sequence derived from alignment at L-elsbetiae_contig5460:113..5934+ (Laminarionema elsbetiae ELsaHSoW15)
ACCATGGACAGATCCCAAAAGCAAAAAGCGGGCACGCTCTCGGTGATGAA
GTACAAGGTCACCATGTCACAGGTGAAAGAAGAGTGACCGGGTGTGTTGG
GTGTGTTGTCTTTATGCGTATGCGTATGCGCACGCGCACGCGTGTGTGTG
TGTGTGTGTGTTGGGTGTTTTTTTTCTAACGTTTTTATGTTTTTATCGGT
CTTGGTGATATTGTCCCCTGCAGAGTGGTTTTGTTACTTCTCGTGCGTGG
TTTTTTCAACAGCGAGATCTCGATCGTTTGCTCGCAGCCTCCGCCCAAGC
GCGGAGGACTTCGGGGAGCATGTCAGTGCGGCCAACTCCTGCCAAGCACG
CGACGTGATCAGTTATACTGTTGCAGCCATGGCAGATATGTATGTAGACG
GCTTGTGTATATTTAAGCGTCAAGCACTGGCATGTAAGACAAGAACCGCT
CTCTCTCTCTCTCTCTCTCTCTCTTTCTCTCGCTGACACACACCGGAGTA
GGAGGCATATTTTGTGTAGCTAATCAGGAGAAACTCTTCAGTGGTGTGTG
TGTGTGCGTGTGTGTGTGCACTGCACTCTGTGCCCTCGCTTACCGCGAAT
CTCTCCCCTGTCGAAAAGTCTCCTGCTGCGGAGGTAGTATATATTTGCCA
CACACCCTCAACTGGTGGCTGGCTCCCGACAGAGTAACGGAATCGTTTTT
TGTTTTGCGTTTTTTGCGTTTTTTTTGCCCCCGCTTACGCTTACAGTATG
TATGTACAGCGTATTCTCTTCCGGTCAACCGCCTGGTGCGGTCGATACCG
ACCAAGTACCCGCTCTACAAATCTAACAGGCGTTATTCTCGTCGGTCGAC
CGGCTGCGCGGTCGGTGCAAAATAGATCCGATAGACTGTTTCAAGAGAAC
ACGCACGATGTACACAAGTATCGACTTTTTATCCACCCATATTGGTACGT
GCCTCAACACATGCGCGCGGTCGATGCTACATAGATGGACTGTTTTAAGA
GAACACGCACGATGTATTTGTGTCGATTGATTTTTTTACATATATCGACT
TTTTCTCCACCCATATTCGTACATGCATACGTTCCTCTACTAAATACATG
CGTGTGCCGTGGGGCAGGCGGCTACGAACGCCCGCGAGTCGACCAGGCGG
CGCAAGGACTCGGTCACGTCGAGGGCCATGCGCAGGGTCAAGCGGCCCGT
GCACCAGAAGTGGACGGAGGCGCTGGACTGGAAGAAGCGGACGTGCCTGC
TCACGGGGGAGAGCCTCTCGCTCTTCAGCGACCCCAAGGACACGGAGCCC
AGCCAGATCGTGTACCTCAGGGGCGCCAACGCGGAGCCCGAGCTTGGGCA
GGTCGGACCAGATTGCTGTAATCTTATATGTTCGCCCTTGTACCATAATC
ATGCGGGTTATCGCACCCCCGTGTAAGCGTTTTTCTATAGCGTATTTGTC
GGGGGCTTTCCGGAGAGGGATCAAATAGCTTTCCGGAGAGGGCATATTGA
TCCCTCTCCGGAAAGCCCCCGACAAATACGCTATAGGCTTTCCGGAGAGG
GCACTAACTGATACCTCTCCGGAAAGCCCCCGACAAATACGCTATAGAAA
AACGCTAACACGGGGGTGCGATAACACGCATGATTACGGTATCCAGATGC
ATACGTAGTGCATACACACACGGAGTTCATGTGTACCTCCATCTACTGTG
TTGTTTATTCTTATTTTTATTTTATTTTCGTGGATGTGAAAATACATGCG
TCGCTGGGGTCTTTTTTTGAGGCGCTTGTTAGGCCTCGAAGCGAGCCTGG
CAGACAAGAACGTTGCTGGGGGGGGGGGCACAACATCCCAACATCGGGTT
GTCCTCCATATGTTCATCGCAGATGAAACACACGAAGGCTGAACACATCC
GCTCGCGGCTTTACGGTTCAAGCACCCACACCTCCAACACTCTCGCTCGC
TGCAAGAAACTACGTATGTACATACATGCTTCATACAATATGTATCTTTC
CGTAATTTGTATGTTTTTTTTTTCGAACCATCAACCATTGTACGGCGTCA
ACACACCTCTTACGCATGCACCCGTTGTATGCGTTGTTGGTGTTGTTATT
GTGTGTGTGTGTGGGGGGGGGGGCATCCATCTTGGCTTTCTCGCTTATCT
CAGGCTTTCAAGGAGTCCGCCTTCCGGGTGGAGACCAACAAGTGGAAGAA
GGGCGACGAGCTAGTTGACGGGACTGAGCGCCGCACCTTCACCTTCTTAG
CCGAGAGCAAGGACGAGAAGGAGCTTTGGCTCTACCAGATCAGGTTGGGG
AGATGTTTTGCTGGTTCGCTGCTGCCTACGTCGGGGTATTTACAAAAGTA
AAAGGCTTTGCGCGGCCACCGAGCTCGACGCTTCTTTTTGTGGCTGATGC
CTGCGCTGGGCGGTGTTCGAAGTCTCGGGAGTTTCGGTTCGGTTCGGTTC
GGTTTCAGCCCCACGAGGCATGTGTCTCGAGCTAGCTAGCTATCTGGCGG
ATCGGGTTGGTTAGACCAGAGTAGATCGGATGAGAGGACAATAACTCGTG
GGCGACACAGAACAACTCGTCGGCGTGGTGTCTTGTCTGTCGCATGGTGG
TATTTTACGATGTCGTCTCGCTGCTGCACTCGACGGCCACACAGCAACCC
GGGCTTGTGGTTTAGGCTTGCGTCAACTAGACATGTACATACATACTCCA
TGCGTACAAGCGAAACCTCGCGCAGCCGCCTGAGAGGTGATGCTTCATGG
TAAGACAGGTGTGCGTACGCACGCACGCACATGGTGCGACTCCGACTCCG
CCCCCTGGGCGCCCCCTCCAGTGTTTCGTAAGACGACTGCGCGCGTGAAT
CAATCATCCCACCCCGCCCTCTAACGTATATAGTCTCCGCTGCCGTGACG
AATGTGCGCGTGCGTCGTCGTTGCACGACCGCGCCTCTCAGGCACTGCAT
CGAGGTGCTCAAGGTGCTCAATGACAAGGCAGGGCCAAGCATGAACAGGT
GTGTACCGTAGGTGCCACAAGATACCACACCCCCTGGACAGATTTTCAAA
GCTCGATTCCTCCCCCGCAAGAAGAGTGCCAGTGCCAAAAAGGGTGGCGT
TGGAAATATCTAGTCAAGGACTGTCCGAAAATTTGTCGTTTGGTGCTGGC
AACTTCTTCGTTTCGGAGTACTGGAGCTCTAAAATTCAGTCCAGGGGGTG
TGGTATCTTGTGTCACCTACGGTGGTTTGTTGTTGTTGTTGTTTTTGTTT
CTCGCGTTGTTTCGTACATCTGAAACTGCTATGGGTGGGTGGATGGTGGG
AAGGAAATAGGGCTTGGGGGGTATGTAGCGACGCCGGCAGCGCTGGTGCC
CCTGTCTTCATGGCGCGCGCGCGAGCGCTGGAATCATGATCGCGCGCTGT
GTTACGTAGTGCCGCCAGCACGACAGAGGGGGTTGCTCTTTGCATGTGTG
CTAACAGAGTAGCGTCGTTCATGGCTGATCTTGGACCTCTTTGGCCGTAC
ATCTACCTTCCTGTCCGTCCGTCTGTGTGCCCCCCCCCCTCTCCCCCCCT
CCTTGTTTTGGGAAGGGTGAGGCAGGGGAGCTCTGGAAGGTCTGGCAGAT
CCAACAGGTTCGGGCCGCCAGACGCCGGGCTTGACGACGGCAAGGATGAC
ATCGACAGCACTCTGATGATCAGGGTGGAAGGGGCTGACGGTGAGTCGCA
TTTATGAACATACTTTAGTATTACCATGTATATATGTAGGTGTACGGAGA
GGTTTTTTGCCGGTGGGGGTTGGGTCGGGGGGGGGCGCCGTTTGCCGCAT
TGGAGTGGGGGAAGGTTGGGAGTGACGCTGCTGTCGGCCGCTTTTTGCCT
GGCACTGTTGTGCTGATGCTGTGCTGGTAGTGTGTGTGTGTGTTTGTGTT
TGTCGTTCGCGCCTTTGTGATCGTGGTGCGGGGCAGAGAGAGACACGTGG
CGGTTGTTGCTCCTGTATTTAGAGTTTAGATGGAGCAGGTTTTTGGGCTG
TGTCTTCATCTGTGATGTGGTTTGTCAAGCGGTTCCTGCGGGGTCGGTTT
CAGCCGGTTGGATGATAGATACCCTCGCCTTCATCCTGCTGGACGTCTTT
GAGATGGGACCACCACTCTATTTGGGATGTATCCCGCGTCCGCTACCGTT
CGAGCTTTTAATCCCTACTCTTAAACCTTCTTGTAGATAGTATTAAAAAA
CAACACACACACACATACACATACATGCGTACGTAGCCAGGACATGGCGT
TCGCCAGACGAAAACCCTGAGCTATTCAGTACGCTCGGTATGTCCTATTC
GTCCACGTTCTCGGCCTGGTTTTCCCAACCCCCGCCCCCCCCCCCCCCCC
CATATAAAAATAACAAGTCGACAGCAGTAGCAGCTGCCCGTTTTGTTCTG
CTACATCCCACCGGCAGGCAATCTATCTCTAAGTCTTGTCGTGTGACCCC
TCTTTCAGCTTGCCCACGACAACAAAGCTTATTTACAGCGCTCCCTCATC
TGCTCCCCCGCCTTTCTCCCCCCCCCCCTCCCCCCGCTCCCACTCTCCTC
TCCCCTCGTAACCCCCTTGATGGGTCGGTGTGTTGGTGCCTTCTGCCGCT
GCCGCTGCCGCTGCCGCCGCCGCCGAGCAGAGCTTATCCTCGAGGAGAAC
TACGGCAAGGTGTACCCGTACTGCCTGGTAGACCTAGACGACCAGAGCCG
CCGGACCAGCTCCGTCCAGTGCGCGTCGGTCTGCCCCTCCTGGGAAGAAG
ACCTTATCTTTGACGTCTCCGTGCAGCAGTGGGTCCTTTCGAATCTCCGG
ATCCAGGTAGGGGTGCTGTGTTCGTGTGTTGTGCCGTGTTTGAGGTGTGT
GGGATGTGTGGGGGGTGTGTGTGTACTGCAGCATGTGTGTGTGTGTGTGG
GGGGGGTCCGCTGCCGTTTTTTTTTCAGACGAATGCCATGGTTTCAGGCG
AGGTGTTTGGCACGATGGCGAGTATGTACGTAAATATGCAGGTGGGGGTG
TTTGCGTTGCGTGGACAGCTGTCTGTTGTGTGTGTTTATATATATGTACA
TGCAGCAGTACTATGGAGTACTTGGTGCTGAAATACTTTTGTGTACCGAT
GATCTCTCAAAGGCGCTTGTTGTGTTCGCCGGCGGAGGAAGCGCTTGTGC
GCCTGCGTGTGCGTTCGTGCGTGTGCGCGTTTTTTTGTACAAGCGTGAGG
CGATGTTGACCATTTTTTTTTGTTAGTCCTCTCGTACGCGAGAACGACCG
CAAACGACGCCTCGTGTGTTTGGCTACTGTAGTGTACTTCATGTAAATAA
GGTGAAAACAAAAGAGCGAGACGTGGAATCTGCGACCTCACGGTGTGCGT
GTGCGTGTGCGTCCACGATGAAAAACATTTGTGTGTGCGGGGGGCGTGGC
GTGTGTTGTTTGTTTCCCTGACGTGTTTGACATATTTTCCCGAAATCGAA
CATCCTTGGTGACCATGTGCTCGCGGGGCACACTCGGAAGTGCAGCATGC
TATTTACACGTATATACACCGAAAAATAAGAGGAATGCTACTCGGTATTG
TAGTCGGGGCGAGGTGTCGGACACCATCAGCACCAAAAACAAAAAGTTAC
TCTGTAGGTATGAAATAGAGAAGACATGAAAAAACGGTATACAGTGATGT
ACCGTGCCAGGCAGGCCCAACAACGCAAGGCTTCCCCAACCCATCTTTTC
ATGCCGGAAGGTTCCACGCCCCGGATTTTTCATCGTGTGTGAGCTTTTTT
GTCCTCCTCCTTCTCCTCCTCCTCCTCCCCCGCTTCTGTTTGTCCAGGTG
TGGCACAAAGACCTGTTCCTGA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig5460:113..5934+

>mRNA_L-elsbetiae_contig5460.13719.1 ID=mRNA_L-elsbetiae_contig5460.13719.1|Name=mRNA_L-elsbetiae_contig5460.13719.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=936bp|location=Sequence derived from alignment at L-elsbetiae_contig5460:113..5934+ (Laminarionema elsbetiae ELsaHSoW15)
ATGGACAGATCCCAAAAGCAAAAAGCGGGCACGCTCTCGGTGATGAAGTA
CAAGGTCACCATGTCACAGATGGACAGATCCCAAAAGCAAAAAGCGGGCA
CGCTCTCGGTGATGAAGTACAAGGTCACCATGTCACAGGCGGCTACGAAC
GCCCGCGAGTCGACCAGGCGGCGCAAGGACTCGGTCACGTCGAGGGCCAT
GCGCAGGGTCAAGCGGCCCGTGCACCAGAAGTGGACGGAGGCGCTGGACT
GGAAGAAGCGGACGTGCCTGCTCACGGGGGAGAGCCTCTCGCTCTTCAGC
GACCCCAAGGACACGGAGCCCAGCCAGATCGTGTACCTCAGGGGCGCCAA
CGCGGAGCCCGAGCTTGGGCAGGCGGCTACGAACGCCCGCGAGTCGACCA
GGCGGCGCAAGGACTCGGTCACGTCGAGGGCCATGCGCAGGGTCAAGCGG
CCCGTGCACCAGAAGTGGACGGAGGCGCTGGACTGGAAGAAGCGGACGTG
CCTGCTCACGGGGGAGAGCCTCTCGCTCTTCAGCGACCCCAAGGACACGG
AGCCCAGCCAGATCGTGTACCTCAGGGGCGCCAACGCGGAGCCCGAGCTT
GGGCAGGCTTTCAAGGAGTCCGCCTTCCGGGTGGAGACCAACAAGTGGAA
GAAGGGCGACGAGCTAGTTGACGGGACTGAGCGCCGCACCTTCACCTTCT
TAGCCGAGAGCAAGGACGAGAAGGAGCTTTGGCTCTACCAGATCAGGCTT
TCAAGGAGTCCGCCTTCCGGGTGGAGACCAACAAGTGGAAGAAGGGCGAC
GAGCTAGTTGACGGGACTGAGCGCCGCACCTTCACCTTCTTAGCCGAGAG
CAAGGACGAGAAGGAGCTTTGGCTCTACCAGATCAGGTGTGGCACAAAGA
CCTGTTCCTGAGTGTGGCACAAAGACCTGTTCCTGA
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