mRNA_L-elsbetiae_contig5119.13182.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig5119.13182.1
Unique NamemRNA_L-elsbetiae_contig5119.13182.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
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BLAST of mRNA_L-elsbetiae_contig5119.13182.1 vs. uniprot
Match: A0A6H5KNQ1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KNQ1_9PHAE)

HSP 1 Score: 75.1 bits (183), Expect = 2.820e-12
Identity = 45/125 (36.00%), Postives = 57/125 (45.60%), Query Frame = 1 
Query:    1 WLREPLEHAAAEGNHGLVDKLLGAGADGSAGWKGCQGRSLLYAAVVGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-NVNFMDPEDKCTILHEAIMGGHEQLAKE 372
            WLR PLEHAA+ GN  LV++LL AGAD  AGW+GC+GR+LL AA +G                                                  +VNF DP  KC++L +AI G H QL  +
Sbjct:   33 WLRVPLEHAASRGNPNLVERLLQAGADAGAGWRGCRGRTLLDAAALGGNADVMSALIRGGAGADVNKVTVSSRRSALHTATCCGHEAAAKRLVLTGADVNFRDPVAKCSVLSQAIQGSHTQLVND 157
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BLAST of mRNA_L-elsbetiae_contig5119.13182.1 vs. uniprot
Match: D8LHQ7_ECTSI (F-box domain and ankyrin repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LHQ7_ECTSI)

HSP 1 Score: 74.3 bits (181), Expect = 5.710e-12
Identity = 34/47 (72.34%), Postives = 39/47 (82.98%), Query Frame = 1 
Query:    1 WLREPLEHAAAEGNHGLVDKLLGAGADGSAGWKGCQGRSLLYAAVVG 141
            WLR PLEHAAA GN  LVDKLLGAGA+G AGW+GC G++LL+AA  G
Sbjct:   76 WLRAPLEHAAATGNRDLVDKLLGAGANGGAGWRGCHGKTLLHAAAEG 122
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BLAST of mRNA_L-elsbetiae_contig5119.13182.1 vs. uniprot
Match: D8LS36_ECTSI (Ankyrin repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LS36_ECTSI)

HSP 1 Score: 72.8 bits (177), Expect = 3.470e-11
Identity = 34/47 (72.34%), Postives = 39/47 (82.98%), Query Frame = 1 
Query:    1 WLREPLEHAAAEGNHGLVDKLLGAGADGSAGWKGCQGRSLLYAAVVG 141
            WLR PLEHAAA GN  LV  LLGAGADGSAGW+GC+GR+L+ AA +G
Sbjct:   33 WLRMPLEHAAARGNLNLVSSLLGAGADGSAGWRGCRGRTLIDAAALG 79
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BLAST of mRNA_L-elsbetiae_contig5119.13182.1 vs. uniprot
Match: D7FXA8_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXA8_ECTSI)

HSP 1 Score: 72.0 bits (175), Expect = 6.500e-11
Identity = 34/47 (72.34%), Postives = 38/47 (80.85%), Query Frame = 1 
Query:    1 WLREPLEHAAAEGNHGLVDKLLGAGADGSAGWKGCQGRSLLYAAVVG 141
            WLR PLEHAAA GN  LVD+LL AG DG AGW+GC+GRSLL AA +G
Sbjct:   31 WLRAPLEHAAASGNLNLVDRLLKAGVDGKAGWRGCRGRSLLDAAALG 77
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BLAST of mRNA_L-elsbetiae_contig5119.13182.1 vs. uniprot
Match: A0A6H5K1F4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K1F4_9PHAE)

HSP 1 Score: 71.6 bits (174), Expect = 9.170e-11
Identity = 44/122 (36.07%), Postives = 55/122 (45.08%), Query Frame = 1 
Query:    1 WLREPLEHAAAEGNHGLVDKLLGAGADGSAGWKGCQGRSLLYAAVVGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-NVNFMDPEDKCTILHEAIMGGHEQL 363
            WLR PLEHAAA GN GLV++LL AGA+G AGW+GC+GR++L AA +G                                                  +VN +DP D C  LH A+ G H  L
Sbjct:   33 WLRAPLEHAAARGNLGLVERLLNAGANGGAGWRGCRGRTMLDAAALGGNPDVVATLLASGCASDVNVVSLSSRRSALHVASVCGHEAAAKKLIIAGADVNRVDPGDACVSLHVAVEGRHGDL 154
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BLAST of mRNA_L-elsbetiae_contig5119.13182.1 vs. uniprot
Match: D8LRZ8_ECTSI (Ankyrin repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LRZ8_ECTSI)

HSP 1 Score: 71.2 bits (173), Expect = 1.130e-10
Identity = 34/47 (72.34%), Postives = 39/47 (82.98%), Query Frame = 1 
Query:    1 WLREPLEHAAAEGNHGLVDKLLGAGADGSAGWKGCQGRSLLYAAVVG 141
            WLR PLEHAAA GN  LV+ LL AGADGSAGWKGC+GR+L+ AA +G
Sbjct:   33 WLRVPLEHAAARGNLNLVNSLLEAGADGSAGWKGCRGRTLIDAAALG 79
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BLAST of mRNA_L-elsbetiae_contig5119.13182.1 vs. uniprot
Match: D8LE08_ECTSI (Similar to ankyrin 2,3/unc44, partial n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LE08_ECTSI)

HSP 1 Score: 69.7 bits (169), Expect = 3.720e-10
Identity = 32/44 (72.73%), Postives = 38/44 (86.36%), Query Frame = 1 
Query:    1 WLREPLEHAAAEGNHGLVDKLLGAGADGSAGWKGCQGRSLLYAA 132
            WLR PLEHAAA GN GLV++LL AGADG AGW+GC+GR++L AA
Sbjct:   33 WLRAPLEHAAARGNIGLVERLLEAGADGRAGWRGCRGRTMLDAA 76
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BLAST of mRNA_L-elsbetiae_contig5119.13182.1 vs. uniprot
Match: A0A6H5KKF4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KKF4_9PHAE)

HSP 1 Score: 68.6 bits (166), Expect = 4.070e-10
Identity = 35/47 (74.47%), Postives = 38/47 (80.85%), Query Frame = 1 
Query:    1 WLREPLEHAAAEGNHGLVDKLLGAGADGSAGWKGCQGRSLLYAAVVG 141
            WLR PLEHAAA+G+  LVDKLLGAGADGSA +KGC GR L  AA VG
Sbjct:   16 WLRVPLEHAAAQGSLVLVDKLLGAGADGSANYKGCAGRILRQAAAVG 62
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BLAST of mRNA_L-elsbetiae_contig5119.13182.1 vs. uniprot
Match: D7G2A3_ECTSI (Similar to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G2A3_ECTSI)

HSP 1 Score: 69.3 bits (168), Expect = 5.280e-10
Identity = 32/47 (68.09%), Postives = 37/47 (78.72%), Query Frame = 1 
Query:    1 WLREPLEHAAAEGNHGLVDKLLGAGADGSAGWKGCQGRSLLYAAVVG 141
            WLR PLEHAA  GN  LVDKLLGAGA+  AGW+GC G++LL+AA  G
Sbjct:   34 WLRAPLEHAAGTGNRDLVDKLLGAGANVGAGWRGCHGKTLLHAAAEG 80
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BLAST of mRNA_L-elsbetiae_contig5119.13182.1 vs. uniprot
Match: D7G692_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G692_ECTSI)

HSP 1 Score: 69.3 bits (168), Expect = 5.750e-10
Identity = 33/47 (70.21%), Postives = 37/47 (78.72%), Query Frame = 1 
Query:    1 WLREPLEHAAAEGNHGLVDKLLGAGADGSAGWKGCQGRSLLYAAVVG 141
            WLR PLEHAAAEGN  L  +L+ AGADGSAGW+GC GR+LL AA  G
Sbjct:   88 WLRAPLEHAAAEGNIDLFTRLMDAGADGSAGWRGCHGRTLLGAAAYG 134
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig5119.13182.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KNQ1_9PHAE2.820e-1236.00Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LHQ7_ECTSI5.710e-1272.34F-box domain and ankyrin repeat protein n=1 Tax=Ec... [more]
D8LS36_ECTSI3.470e-1172.34Ankyrin repeat protein n=1 Tax=Ectocarpus siliculo... [more]
D7FXA8_ECTSI6.500e-1172.34Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5K1F4_9PHAE9.170e-1136.07Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LRZ8_ECTSI1.130e-1072.34Ankyrin repeat protein n=1 Tax=Ectocarpus siliculo... [more]
D8LE08_ECTSI3.720e-1072.73Similar to ankyrin 2,3/unc44, partial n=1 Tax=Ecto... [more]
A0A6H5KKF4_9PHAE4.070e-1074.47Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G2A3_ECTSI5.280e-1068.09Similar to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus si... [more]
D7G692_ECTSI5.750e-1070.21Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig5119contigL-elsbetiae_contig5119:6384..7095 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score75.1
Seed ortholog evalue3.2e-11
Seed eggNOG ortholog2880.D8LHQ7
KEGG koko:K21440
Hectar predicted targeting categoryother localisation
GOsGO:0001508,GO:0002027,GO:0002262,GO:0002376,GO:0002520,GO:0003008,GO:0003012,GO:0003013,GO:0003015,GO:0003205,GO:0003230,GO:0003279,GO:0003283,GO:0003674,GO:0005198,GO:0005200,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005783,GO:0005829,GO:0005856,GO:0005886,GO:0005911,GO:0005938,GO:0006725,GO:0006778,GO:0006779,GO:0006807,GO:0006810,GO:0006811,GO:0006812,GO:0006816,GO:0006873,GO:0006874,GO:0006875,GO:0006887,GO:0006888,GO:0006928,GO:0006936,GO:0006937,GO:0006941,GO:0006942,GO:0006996,GO:0007009,GO:0007010,GO:0007154,GO:0007163,GO:0007165,GO:0007267,GO:0007275,GO:0007507,GO:0008015,GO:0008016,GO:0008092,GO:0008093,GO:0008104,GO:0008150,GO:0008152,GO:0009058,GO:0009893,GO:0009987,GO:0010256,GO:0010468,GO:0010522,GO:0010604,GO:0010628,GO:0010638,GO:0010646,GO:0010880,GO:0010881,GO:0010882,GO:0010959,GO:0012505,GO:0014704,GO:0014731,GO:0015672,GO:0016020,GO:0016043,GO:0016192,GO:0016323,GO:0016528,GO:0016529,GO:0018130,GO:0019222,GO:0019438,GO:0019722,GO:0019725,GO:0019899,GO:0019900,GO:0019901,GO:0019932,GO:0022898,GO:0023051,GO:0023052,GO:0030001,GO:0030003,GO:0030011,GO:0030016,GO:0030017,GO:0030018,GO:0030029,GO:0030048,GO:0030054,GO:0030097,GO:0030099,GO:0030154,GO:0030218,GO:0030315,GO:0030424,GO:0030507,GO:0030673,GO:0030674,GO:0030863,GO:0031252,GO:0031253,GO:0031256,GO:0031430,GO:0031647,GO:0031672,GO:0031674,GO:0032409,GO:0032411,GO:0032412,GO:0032414,GO:0032501,GO:0032502,GO:0032589,GO:0032879,GO:0032940,GO:0032970,GO:0033013,GO:0033014,GO:0033036,GO:0033043,GO:0033267,GO:0033292,GO:0033365,GO:0034101,GO:0034394,GO:0034613,GO:0034641,GO:0034762,GO:0034764,GO:0034765,GO:0034767,GO:0035088,GO:0035090,GO:0035556,GO:0035637,GO:0036309,GO:0036371,GO:0042383,GO:0042391,GO:0042592,GO:0042692,GO:0042995,GO:0043005,GO:0043034,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043266,GO:0043268,GO:0043269,GO:0043270,GO:0043292,GO:0044057,GO:0044093,GO:0044237,GO:0044249,GO:0044271,GO:0044291,GO:0044304,GO:0044325,GO:0044422,GO:0044424,GO:0044425,GO:0044444,GO:0044448,GO:0044449,GO:0044456,GO:0044459,GO:0044463,GO:0044464,GO:0045197,GO:0045199,GO:0045202,GO:0045211,GO:0046483,GO:0046903,GO:0046907,GO:0048193,GO:0048468,GO:0048513,GO:0048518,GO:0048522,GO:0048534,GO:0048731,GO:0048821,GO:0048856,GO:0048869,GO:0048872,GO:0048878,GO:0050789,GO:0050794,GO:0050801,GO:0050821,GO:0050896,GO:0051049,GO:0051050,GO:0051117,GO:0051128,GO:0051130,GO:0051179,GO:0051186,GO:0051188,GO:0051234,GO:0051239,GO:0051270,GO:0051279,GO:0051282,GO:0051641,GO:0051649,GO:0051716,GO:0051899,GO:0051924,GO:0051928,GO:0055001,GO:0055065,GO:0055072,GO:0055074,GO:0055076,GO:0055080,GO:0055082,GO:0055117,GO:0060047,GO:0060048,GO:0060090,GO:0060255,GO:0060306,GO:0060307,GO:0060341,GO:0061024,GO:0061061,GO:0061245,GO:0061337,GO:0061515,GO:0065007,GO:0065008,GO:0065009,GO:0070252,GO:0070296,GO:0070727,GO:0070838,GO:0070972,GO:0071704,GO:0071840,GO:0071944,GO:0072359,GO:0072503,GO:0072507,GO:0072511,GO:0072657,GO:0072659,GO:0086001,GO:0086002,GO:0086003,GO:0086004,GO:0086005,GO:0086010,GO:0086012,GO:0086014,GO:0086015,GO:0086018,GO:0086019,GO:0086026,GO:0086046,GO:0086065,GO:0086066,GO:0086070,GO:0086091,GO:0090257,GO:0097060,GO:0097458,GO:0098590,GO:0098771,GO:0098794,GO:0098900,GO:0098901,GO:0098907,GO:0098910,GO:0099080,GO:0099081,GO:0099512,GO:0099568,GO:0099623,GO:0120025,GO:0120038,GO:1901016,GO:1901018,GO:1901019,GO:1901021,GO:1901360,GO:1901362,GO:1901379,GO:1901381,GO:1901564,GO:1901566,GO:1901576,GO:1903115,GO:1903169,GO:1903522,GO:1903779,GO:1904062,GO:1904064,GO:1904427,GO:1990778,GO:2001257,GO:2001259
EggNOG free text desc.response to abiotic stimulus
EggNOG OGsCOG0666@1,KOG4177@2759
COG Functional cat.G
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04131
Exons1
Model size713
Cds size468
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816522.4182878-CDS-L-elsbetiae_contig5119:6383..68511622816522.4182878-CDS-L-elsbetiae_contig5119:6383..6851Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5119 6384..6851 +
1691683620.6849198-CDS-L-elsbetiae_contig5119:6383..68511691683620.6849198-CDS-L-elsbetiae_contig5119:6383..6851Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig5119 6384..6851 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816522.429536-UTR-L-elsbetiae_contig5119:6851..70951622816522.429536-UTR-L-elsbetiae_contig5119:6851..7095Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5119 6852..7095 +
1691683620.6980834-UTR-L-elsbetiae_contig5119:6851..70951691683620.6980834-UTR-L-elsbetiae_contig5119:6851..7095Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig5119 6852..7095 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig5119.13182.1prot_L-elsbetiae_contig5119.13182.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig5119 6384..6851 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig5119.13182.1

>prot_L-elsbetiae_contig5119.13182.1 ID=prot_L-elsbetiae_contig5119.13182.1|Name=mRNA_L-elsbetiae_contig5119.13182.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=156bp
WLREPLEHAAAEGNHGLVDKLLGAGADGSAGWKGCQGRSLLYAAVVGGSE
GVVSSLLRAGAQVDVNVLSHVSSGRSHQAASSGYEAVARLLILAGANVNF
MDPEDKCTILHEAIMGGHEQLAKEAEQMLRHWTLMVLLLFMQLLGKAWTG
SSPSS*
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mRNA from alignment at L-elsbetiae_contig5119:6384..7095+

Legend: CDSpolypeptideUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig5119.13182.1 ID=mRNA_L-elsbetiae_contig5119.13182.1|Name=mRNA_L-elsbetiae_contig5119.13182.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=712bp|location=Sequence derived from alignment at L-elsbetiae_contig5119:6384..7095+ (Laminarionema elsbetiae ELsaHSoW15)
TGGCTTCGGGAGCCGCTCGAACACGCCGCCGCAGAGGGCAACCATGGCCT
GGTCGACAAGCTGTTAGGAGCCGGGGCGGACGGGAGTGCAGGATGGAAGG
GTTGCCAAGGCCGAAGCCTTCTCTACGCTGCCGTTGTCGGTGGGAGCGAA
GGCGTGGTATCTTCCTTGCTTCGAGCAGGAGCTCAGGTGGATGTGAATGT
TTTGTCACATGTGTCCTCCGGTCGGTCGCACCAGGCAGCATCGAGCGGCT
ACGAGGCTGTTGCGAGGCTTTTGATTTTGGCGGGCGCGAATGTCAACTTT
ATGGACCCCGAAGACAAGTGTACCATACTCCACGAAGCGATTATGGGTGG
TCATGAACAGCTTGCGAAGGAGGCGGAGCAAATGTTGAGGCACTGGACTC
TGATGGTACTACTGCTCTTCATGCAGCTGCTGGGAAAGGCTTGGACGGGA
TCGTCTCCATCCTCTTGATTCGAGGGGCGGACAAGGACAAAGTCGACCTT
GACGGAGATTCTGCTTTGATGTGGGCGTCTGGTGCCAACGAGTCTAGCGA
GGCTCATCCGACTGTCGTGGAGACTCTGCTGGCCGCTGGTGCTGATTTCA
ACATCCGTCGGTCTGACAGCAGTGGATACTCGGCGCTCCACTGGGCTTCT
CGTTCTGGGTGCGTTCTCAGTATCCAAGCTCTTGTTCGGCATGGGGCAGA
CGTGAACTCTCG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig5119:6384..7095+

>mRNA_L-elsbetiae_contig5119.13182.1 ID=mRNA_L-elsbetiae_contig5119.13182.1|Name=mRNA_L-elsbetiae_contig5119.13182.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=936bp|location=Sequence derived from alignment at L-elsbetiae_contig5119:6384..7095+ (Laminarionema elsbetiae ELsaHSoW15)
TGGCTTCGGGAGCCGCTCGAACACGCCGCCGCAGAGGGCAACCATGGCCT
GGTCGACAAGCTGTTAGGAGCCGGGGCGGACGGGAGTGCAGGATGGAAGG
GTTGCCAAGGCCGAAGCCTTCTCTACGCTGCCGTTGTCGGTGGGAGCGAA
GGCGTGGTATCTTCCTTGCTTCGAGCAGGAGCTCAGGTGGATGTGAATGT
TTTGTCACATGTGTCCTCCGGTCGGTCGCACCAGGCAGCATCGAGCGGCT
ACGAGGCTGTTGCGAGGCTTTTGATTTTGGCGGGCGCGAATGTCAACTTT
ATGGACCCCGAAGACAAGTGTACCATACTCCACGAAGCGATTATGGGTGG
TCATGAACAGCTTGCGAAGGAGGCGGAGCAAATGTTGAGGCACTGGACTC
TGATGGTACTACTGCTCTTCATGCAGCTGCTGGGAAAGGCTTGGACGGGA
TCGTCTCCATCCTCTTGATGGCTTCGGGAGCCGCTCGAACACGCCGCCGC
AGAGGGCAACCATGGCCTGGTCGACAAGCTGTTAGGAGCCGGGGCGGACG
GGAGTGCAGGATGGAAGGGTTGCCAAGGCCGAAGCCTTCTCTACGCTGCC
GTTGTCGGTGGGAGCGAAGGCGTGGTATCTTCCTTGCTTCGAGCAGGAGC
TCAGGTGGATGTGAATGTTTTGTCACATGTGTCCTCCGGTCGGTCGCACC
AGGCAGCATCGAGCGGCTACGAGGCTGTTGCGAGGCTTTTGATTTTGGCG
GGCGCGAATGTCAACTTTATGGACCCCGAAGACAAGTGTACCATACTCCA
CGAAGCGATTATGGGTGGTCATGAACAGCTTGCGAAGGAGGCGGAGCAAA
TGTTGAGGCACTGGACTCTGATGGTACTACTGCTCTTCATGCAGCTGCTG
GGAAAGGCTTGGACGGGATCGTCTCCATCCTCTTGA
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