mRNA_L-elsbetiae_contig193.5709.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig193.5709.1
Unique NamemRNA_L-elsbetiae_contig193.5709.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig193.5709.1 vs. uniprot
Match: A0A6H5KED2_9PHAE (cGMP-dependent protein kinase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KED2_9PHAE)

HSP 1 Score: 273 bits (697), Expect = 4.180e-81
Identity = 150/218 (68.81%), Postives = 176/218 (80.73%), Query Frame = 1
Query:    1 MQQAALQTACYREQKAVISGQQXXXXQPA---------------RVGTESSSNFASVRALAAVRGSTRSSFRFTDQVTFAGDALGETSGRGVNDYRVCEKVGEGSFGAVYKVVHAHSGKTYAMKVITKPKQGGQKRELLERSLRREVSVMQMLKHPNVVTLWEVIDDPRSRKVYMIQEFMEGGSLFKEKYAVEPLAEQVAISKFVQAARGLQYIHSFG 609
            M+QAALQTACYREQ+  ISG Q XXX                  R G  SS++F+++RALAAVRG+TRSSFR T Q+TFAG + GETSGR +NDYRVCE++GEGSFG VYKVV  H+G+  AMKVITK ++GGQKRELLER+LRREVSVM++L+HPNVVTLWEVIDDPRSRKVYMIQ+FME GSL KE+Y V+P+ E  AISKF+QAARGLQYIHSFG
Sbjct: 1070 MEQAALQTACYREQRKNISGLQQXXXXXXXXXHVANEGSKFATPRHGVGSSNDFSTLRALAAVRGATRSSFRSTGQLTFAGQSNGETSGRVINDYRVCERIGEGSFGVVYKVVRVHTGEVNAMKVITKSRRGGQKRELLERTLRREVSVMKILRHPNVVTLWEVIDDPRSRKVYMIQDFMERGSLLKEQYEVDPIPEHTAISKFIQAARGLQYIHSFG 1287          
BLAST of mRNA_L-elsbetiae_contig193.5709.1 vs. uniprot
Match: D7FK08_ECTSI (cGMP-dependent protein kinase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FK08_ECTSI)

HSP 1 Score: 267 bits (683), Expect = 3.200e-79
Identity = 135/174 (77.59%), Postives = 156/174 (89.66%), Query Frame = 1
Query:   88 RVGTESSSNFASVRALAAVRGSTRSSFRFTDQVTFAGDALGETSGRGVNDYRVCEKVGEGSFGAVYKVVHAHSGKTYAMKVITKPKQGGQKRELLERSLRREVSVMQMLKHPNVVTLWEVIDDPRSRKVYMIQEFMEGGSLFKEKYAVEPLAEQVAISKFVQAARGLQYIHSFG 609
            R G  SS++F+++RALAAVRG+TRSSFR T Q+TFAGD  GETSGR +NDYRVCE++GEGSFG VYKVVH H+G+  AMKVITKP++GGQKRELLER+LRREVSVMQML+HPNVVTLWEVIDDPRSRKVYMIQ+FME GSL KE+Y V+P+ E  AISKF+QAARGLQYIHSFG
Sbjct:  987 RHGVGSSNDFSTLRALAAVRGATRSSFRSTGQLTFAGDTKGETSGRVINDYRVCERIGEGSFGVVYKVVHVHTGEVNAMKVITKPRRGGQKRELLERTLRREVSVMQMLRHPNVVTLWEVIDDPRSRKVYMIQDFMERGSLLKEQYEVDPIPEHTAISKFIQAARGLQYIHSFG 1160          
BLAST of mRNA_L-elsbetiae_contig193.5709.1 vs. uniprot
Match: A0A6H5LNX5_9PHAE (Protein kinase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LNX5_9PHAE)

HSP 1 Score: 116 bits (290), Expect = 6.110e-27
Identity = 83/227 (36.56%), Postives = 121/227 (53.30%), Query Frame = 1
Query:    1 MQQAALQTACYREQKA--VISGQQXXXXQPARVGTESSSNFASVRALAAVRGST---------------RSSFRFTDQVTFAGDALGETSGRGVNDYRVCEKVGEGSFGAVYKVVHAHSGKTYAMKVITKPKQGGQK-------RELLERSLRREVSVMQMLKHPNVVTLWEVIDDPRSRKVYMIQEFMEGGSLFKEKYAVEPLAEQVAISKFVQAARGLQYIHSFG 609
            +++ A Q A YREQ+A  +   Q+       + G       A VR ++  R  T               RSSFR T +V       G      +N YR+  ++G+GS+G+V+      +G  YAMKVI K K+GG +        + L  +LRREV+VM+ L+HPN+VTLWEVIDDP+++++YMIQE++  G L  E   V P+  + A  KFV   RGL ++H  G
Sbjct:   10 LEEVAYQRAFYREQRAQQLEEEQEKQVCGVGKPGGRGRWTTAEVRGVSPTRSLTPPCGQSASSSSHSLRRSSFRETVKVVKRKLNNGTII---INKYRIICELGKGSYGSVHLCRDGETGIEYAMKVIDKRKRGGMRSRQGQGGHQHLAETLRREVAVMKKLRHPNIVTLWEVIDDPKAQQLYMIQEYVADGPLLPEGVVVSPMDTEEARDKFVGCIRGLHHLHQHG 233          
BLAST of mRNA_L-elsbetiae_contig193.5709.1 vs. uniprot
Match: D8LIK7_ECTSI (cGMP-dependent protein kinase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LIK7_ECTSI)

HSP 1 Score: 113 bits (282), Expect = 2.220e-25
Identity = 58/134 (43.28%), Postives = 88/134 (65.67%), Query Frame = 1
Query:  229 VNDYRVCEKVGEGSFGAVYKVVHAHSGKTYAMKVITKPKQGGQK-------RELLERSLRREVSVMQMLKHPNVVTLWEVIDDPRSRKVYMIQEFMEGGSLFKEKYAVEPLAEQVAISKFVQAARGLQYIHSFG 609
            +N YR+  ++G+GS+G+V+      +G  YAMKV+ K K+GG +        + L  +LRREV+VM+ L+HPN+VTLWEVIDDP+++++YMIQE++  G L  E   V P+  + A  KF+   RGL ++H  G
Sbjct:  566 INKYRIICELGKGSYGSVHLCRDGETGMEYAMKVMDKRKRGGIRSRQGQGGHQHLAETLRREVAVMKKLRHPNIVTLWEVIDDPKAQQLYMIQEYVADGPLLPEGVVVSPMDTEEARDKFLGCIRGLHHLHQHG 699          
BLAST of mRNA_L-elsbetiae_contig193.5709.1 vs. uniprot
Match: A0A482SUA7_9ARCH (Cyclic nucleotide-binding domain-containing protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SUA7_9ARCH)

HSP 1 Score: 98.2 bits (243), Expect = 1.300e-20
Identity = 57/133 (42.86%), Postives = 79/133 (59.40%), Query Frame = 1
Query:  211 ETSGRGVNDYRVCEKVGEGSFGAVYKVVHAHSGKTYAMKVITKPKQGGQKRELLERSLRREVSVMQMLKHPNVVTLWEVIDDPRSRKVYMIQEFMEGGSLFKEKYAVEPLAEQVAISKFVQAARGLQYIHSFG 609
            E+  R +N Y V  ++G+GSFG VY      + K YAMK I++P       E    ++R+E++VM+ LKH NVV L EVIDD  +RK+++IQEFMEGG L  +    EPL   +A   F    RG+ Y+H  G
Sbjct:  159 ESGSRVINKYIVERELGKGSFGEVYLCRDIETNKEYAMKQISRPTTSSWNDES-SNAIRQEIAVMKRLKHQNVVGLHEVIDDQNARKIFLIQEFMEGGPLMDDAETCEPLDPNLARKYFRDILRGVCYLHYEG 290          
BLAST of mRNA_L-elsbetiae_contig193.5709.1 vs. uniprot
Match: A0A0L0DPX6_THETB (Protein kinase domain-containing protein n=1 Tax=Thecamonas trahens ATCC 50062 TaxID=461836 RepID=A0A0L0DPX6_THETB)

HSP 1 Score: 95.1 bits (235), Expect = 4.190e-19
Identity = 48/132 (36.36%), Postives = 81/132 (61.36%), Query Frame = 1
Query:  229 VNDYRVCEKVGEGSFGAVYKVVHAHSGKTYAMKV-----ITKPKQGGQKRELLERSLRREVSVMQMLKHPNVVTLWEVIDDPRSRKVYMIQEFMEGGSLFKEKYAVEPLAEQVAISKFVQAARGLQYIHSFG 609
            +NDY + + +G G++G V   +HA  G   A+K+     +++ ++GG     L   +RRE+++M+ L HPN+V L+EVIDDP + K+Y++ E++ GG+L  +    EP++E  A   F     GL+Y+HS G
Sbjct:  490 INDYILLKTLGRGTYGKVKLGIHAQDGTRVAVKICNKFLLSRKRKGGLLSASLLEDVRREIAIMKKLDHPNIVRLFEVIDDPSNDKLYLVMEYVPGGALLGDVIRCEPMSEARARRYFRDVVAGLEYLHSSG 621          
BLAST of mRNA_L-elsbetiae_contig193.5709.1 vs. uniprot
Match: A0A835YIE0_9STRA (cGMP-dependent protein kinase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YIE0_9STRA)

HSP 1 Score: 91.3 bits (225), Expect = 9.020e-18
Identity = 58/162 (35.80%), Postives = 84/162 (51.85%), Query Frame = 1
Query:  238 YRVCEKVGEGSFGAVYKVVHAHSGKTYA---------MKVITKPKQGGQKRELLERSLRREVSVMQMLKHPNVVTLWEVIDDPR-----------------------------SRKVYMIQEFMEGGSLFKEKYAVEPLAEQVAISKFVQAARGLQYIHSFG 609
            Y + E++G GS+G+VYK     +G   A         +KV+ K ++G +  ++LE SLRREV+ ++ ++HPN+VTL EVI+DP+                               KVYM+QE+ME G++    Y V PL E  A   FV A RGL Y+H  G
Sbjct:  776 YEMREEIGRGSYGSVYKCFDRETGMFKAIKASCDISVLKVLNKKQRGKRGTQMLE-SLRREVAALKKMRHPNIVTLLEVINDPKXXXXXXXXXXXXXXXXXXXXXXXGISDPKGEKVYMVQEYMELGTILPGSYQVTPLPEDEARQYFVHAVRGLHYLHMHG 936          
BLAST of mRNA_L-elsbetiae_contig193.5709.1 vs. uniprot
Match: A0A367ITW2_RHIAZ (Protein kinase domain-containing protein (Fragment) n=4 Tax=Rhizopus TaxID=4842 RepID=A0A367ITW2_RHIAZ)

HSP 1 Score: 90.1 bits (222), Expect = 1.930e-17
Identity = 49/126 (38.89%), Postives = 80/126 (63.49%), Query Frame = 1
Query:  223 RGVNDYRVCEKVGEGSFGAVYKVVHAHSGKTYAMKVITKPKQGGQKRELLERSLRREVSVMQMLKHPNVVTLWEVIDDPRSRKVYMIQEFMEGGSLFKEKYAVEPLAEQVAISKFVQAARGLQYIH 600
            R + DY V + +G+G+ G V   VH ++G+  A+K+I+K    G     +E+++RRE+++M+++ HPNV++L +VIDDP S  +Y+I E++EGG LF+   +   L+E  A   F Q   GL Y H
Sbjct:   35 RYIGDYIVGKTLGKGASGRVKLGVHKNTGEQVAIKIISKTHLAGN--PAIEKAVRREIAIMKLINHPNVMSLIDVIDDPASSDLYLILEYVEGGELFEYLVSKGRLSESEARHHFQQIILGLDYCH 158          
BLAST of mRNA_L-elsbetiae_contig193.5709.1 vs. uniprot
Match: A0A024UTS4_9STRA (cGMP-dependent protein kinase n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UTS4_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 4.060e-17
Identity = 58/135 (42.96%), Postives = 83/135 (61.48%), Query Frame = 1
Query:  202 ALGETSGRGVNDYRVCEKVGEGSFGAVYKVVHAHSGKTYAMKVITKP--KQGGQKRELLERSLRREVSVMQMLKHPNVVTLWEVIDDPRSRKVYMIQEFMEGGSLFKEKYAVEPLAEQVAISKFVQAARGLQYIH 600
            A GET    VN Y +  ++G+GSFG V    +   G  YA+K++ K   ++   + + L+ +LRREV++M+ L H NVV L EVIDDP S+KVY++QE++E G+L  E     PLAE+VA         GLQY+H
Sbjct:  468 ANGETV---VNKYVIKGELGKGSFGTVKLCANEEDGLLYAVKIMHKTFVQRMANREDSLQDALRREVAIMKKLDHRNVVRLVEVIDDPSSQKVYLVQEYVEKGNL-AEVAGGAPLAEEVARKYLRDLLCGLQYLH 598          
BLAST of mRNA_L-elsbetiae_contig193.5709.1 vs. uniprot
Match: A0A3R6YD84_9STRA (cGMP-dependent protein kinase n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A3R6YD84_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 4.180e-17
Identity = 58/135 (42.96%), Postives = 83/135 (61.48%), Query Frame = 1
Query:  202 ALGETSGRGVNDYRVCEKVGEGSFGAVYKVVHAHSGKTYAMKVITKP--KQGGQKRELLERSLRREVSVMQMLKHPNVVTLWEVIDDPRSRKVYMIQEFMEGGSLFKEKYAVEPLAEQVAISKFVQAARGLQYIH 600
            A GET    VN Y +  ++G+GSFG V    +   G  YA+K++ K   ++   + + L+ +LRREV++M+ L H NVV L EVIDDP S+KVY++QE++E G+L  E     PLAE+VA         GLQY+H
Sbjct: 1084 ANGETV---VNKYVIKGELGKGSFGTVKLCANEEDGLLYAVKIMHKTFVQRMANREDSLQDALRREVAIMKKLDHRNVVRLVEVIDDPSSQKVYLVQEYVEKGNL-AEVAGGAPLAEEVARKYLRDLLCGLQYLH 1214          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig193.5709.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KED2_9PHAE4.180e-8168.81cGMP-dependent protein kinase n=1 Tax=Ectocarpus s... [more]
D7FK08_ECTSI3.200e-7977.59cGMP-dependent protein kinase n=1 Tax=Ectocarpus s... [more]
A0A6H5LNX5_9PHAE6.110e-2736.56Protein kinase domain-containing protein n=1 Tax=E... [more]
D8LIK7_ECTSI2.220e-2543.28cGMP-dependent protein kinase n=1 Tax=Ectocarpus s... [more]
A0A482SUA7_9ARCH1.300e-2042.86Cyclic nucleotide-binding domain-containing protei... [more]
A0A0L0DPX6_THETB4.190e-1936.36Protein kinase domain-containing protein n=1 Tax=T... [more]
A0A835YIE0_9STRA9.020e-1835.80cGMP-dependent protein kinase n=1 Tax=Tribonema mi... [more]
A0A367ITW2_RHIAZ1.930e-1738.89Protein kinase domain-containing protein (Fragment... [more]
A0A024UTS4_9STRA4.060e-1742.96cGMP-dependent protein kinase n=2 Tax=Aphanomyces ... [more]
A0A3R6YD84_9STRA4.180e-1742.96cGMP-dependent protein kinase n=1 Tax=Aphanomyces ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig193contigL-elsbetiae_contig193:16579..17431 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score271.2
Seed ortholog evalue4e-70
Seed eggNOG ortholog2880.D7FK08
KEGG koko:K07359
KEGG Pathwayko04140,ko04152,ko04211,ko04920,ko04921,ko05034,map04140,map04152,map04211,map04920,map04921,map05034
Hectar predicted targeting categoryother localisation
EggNOG free text desc.peptidyl-threonine phosphorylation
EggNOG OGsKOG0585@1,KOG0585@2759
EC2.7.11.17
COG Functional cat.T
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01001
Exons2
Model size611
Cds size609
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815800.3467455-CDS-L-elsbetiae_contig193:16578..169051622815800.3467455-CDS-L-elsbetiae_contig193:16578..16905Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig193 16579..16905 +
1691683110.815519-CDS-L-elsbetiae_contig193:16578..169051691683110.815519-CDS-L-elsbetiae_contig193:16578..16905Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig193 16579..16905 +
1622815800.3588078-CDS-L-elsbetiae_contig193:17149..174311622815800.3588078-CDS-L-elsbetiae_contig193:17149..17431Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig193 17150..17431 +
1691683110.828525-CDS-L-elsbetiae_contig193:17149..174311691683110.828525-CDS-L-elsbetiae_contig193:17149..17431Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig193 17150..17431 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig193.5709.1prot_L-elsbetiae_contig193.5709.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig193 16579..17431 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig193.5709.1

>prot_L-elsbetiae_contig193.5709.1 ID=prot_L-elsbetiae_contig193.5709.1|Name=mRNA_L-elsbetiae_contig193.5709.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=203bp
MQQAALQTACYREQKAVISGQQQQQQQPARVGTESSSNFASVRALAAVRG
STRSSFRFTDQVTFAGDALGETSGRGVNDYRVCEKVGEGSFGAVYKVVHA
HSGKTYAMKVITKPKQGGQKRELLERSLRREVSVMQMLKHPNVVTLWEVI
DDPRSRKVYMIQEFMEGGSLFKEKYAVEPLAEQVAISKFVQAARGLQYIH
SFG
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mRNA from alignment at L-elsbetiae_contig193:16579..17431+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig193.5709.1 ID=mRNA_L-elsbetiae_contig193.5709.1|Name=mRNA_L-elsbetiae_contig193.5709.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=853bp|location=Sequence derived from alignment at L-elsbetiae_contig193:16579..17431+ (Laminarionema elsbetiae ELsaHSoW15)
ATGCAGCAGGCAGCTCTGCAGACCGCCTGTTATCGCGAGCAAAAGGCTGT GATCTCAGGGCAGCAGCAGCAGCAGCAGCAGCCTGCAAGAGTGGGCACCG AGTCGTCGTCAAACTTTGCCAGCGTGCGGGCATTGGCAGCAGTGCGGGGT AGCACGCGCTCCAGCTTCCGATTTACCGACCAGGTCACATTTGCAGGAGA TGCACTGGGGGAGACAAGCGGGCGAGGGGTCAATGACTACCGCGTGTGTG AGAAGGTCGGGGAGGGCAGCTTCGGGGCGGTGTACAAGGTGGTTCACGCA CACTCCGGGAAAACTTACGCCATGAAGGTGTGTGTTCAGACTCTCGCCCA TGCCGTGCGTGCCTCGCCCGTCAGGGCTGAGCGCAATTGACGAGTCTCGG TTTAGCTTCCCCCAAGTATTTCGTTTTTTTAGTTATTCCGCGCTTTGGAC TTAAACGCGCGCTGCTTATCCAAGCTGCCGGGCCTCGGTCAGTCACTATC TATCGCACAACCTTATCATTTTTCAGAAAAAAAAACTCCAATCATCGCCG CAATGATTTTTTTTCCCAAAGGTGATTACGAAACCGAAGCAGGGCGGGCA GAAACGTGAACTCCTCGAGCGAAGCCTCCGGCGAGAGGTGTCGGTGATGC AGATGCTGAAGCACCCAAACGTCGTCACTCTTTGGGAGGTCATCGACGAC CCTCGCTCACGGAAGGTTTACATGATCCAAGAGTTCATGGAAGGCGGCTC ATTGTTCAAGGAGAAGTACGCGGTGGAGCCCCTAGCCGAGCAGGTCGCCA TTTCTAAGTTTGTCCAAGCTGCTAGGGGTCTACAGTACATCCACAGCTTC GGT
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Coding sequence (CDS) from alignment at L-elsbetiae_contig193:16579..17431+

>mRNA_L-elsbetiae_contig193.5709.1 ID=mRNA_L-elsbetiae_contig193.5709.1|Name=mRNA_L-elsbetiae_contig193.5709.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=1218bp|location=Sequence derived from alignment at L-elsbetiae_contig193:16579..17431+ (Laminarionema elsbetiae ELsaHSoW15)
ATGCAGCAGGCAGCTCTGCAGACCGCCTGTTATCGCGAGCAAAAGGCTGT
GATCTCAGGGCAGCAGCAGCAGCAGCAGCAGCCTGCAAGAGTGGGCACCG
AGTCGTCGTCAAACTTTGCCAGCGTGCGGGCATTGGCAGCAGTGCGGGGT
AGCACGCGCTCCAGCTTCCGATTTACCGACCAGGTCACATTTGCAGGAGA
TGCACTGGGGGAGACAAGCGGGCGAGGGGTCAATGACTACCGCGTGTGTG
AGAAGGTCGGGGAGGGCAGCTTCGGGGCGGTGTACAAGGTGGTTCACGCA
CACTCCGGGAAAACTTACGCCATGAAGATGCAGCAGGCAGCTCTGCAGAC
CGCCTGTTATCGCGAGCAAAAGGCTGTGATCTCAGGGCAGCAGCAGCAGC
AGCAGCAGCCTGCAAGAGTGGGCACCGAGTCGTCGTCAAACTTTGCCAGC
GTGCGGGCATTGGCAGCAGTGCGGGGTAGCACGCGCTCCAGCTTCCGATT
TACCGACCAGGTCACATTTGCAGGAGATGCACTGGGGGAGACAAGCGGGC
GAGGGGTCAATGACTACCGCGTGTGTGAGAAGGTCGGGGAGGGCAGCTTC
GGGGCGGTGTACAAGGTGGTTCACGCACACTCCGGGAAAACTTACGCCAT
GAAGGTGATTACGAAACCGAAGCAGGGCGGGCAGAAACGTGAACTCCTCG
AGCGAAGCCTCCGGCGAGAGGTGTCGGTGATGCAGATGCTGAAGCACCCA
AACGTCGTCACTCTTTGGGAGGTCATCGACGACCCTCGCTCACGGAAGGT
TTACATGATCCAAGAGTTCATGGAAGGCGGCTCATTGTTCAAGGAGAAGT
ACGCGGTGGAGCCCCTAGCCGAGCAGGTCGCCATTTCTAAGTTTGTCCAA
GCTGCTAGGGGTCTACAGTACATCCACAGCTTCGGTGTGATTACGAAACC
GAAGCAGGGCGGGCAGAAACGTGAACTCCTCGAGCGAAGCCTCCGGCGAG
AGGTGTCGGTGATGCAGATGCTGAAGCACCCAAACGTCGTCACTCTTTGG
GAGGTCATCGACGACCCTCGCTCACGGAAGGTTTACATGATCCAAGAGTT
CATGGAAGGCGGCTCATTGTTCAAGGAGAAGTACGCGGTGGAGCCCCTAG
CCGAGCAGGTCGCCATTTCTAAGTTTGTCCAAGCTGCTAGGGGTCTACAG
TACATCCACAGCTTCGGT
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