mRNA_L-elsbetiae_contig19097.5623.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig19097.5623.1
Unique NamemRNA_L-elsbetiae_contig19097.5623.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig19097.5623.1 vs. uniprot
Match: D7FQK3_ECTSI (Argonaute 1 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FQK3_ECTSI)

HSP 1 Score: 242 bits (618), Expect = 2.410e-72
Identity = 118/126 (93.65%), Postives = 125/126 (99.21%), Query Frame = 1
Query:   43 QTSQNAYDCITALDISLRHAPSMKLTCVGRSFFTPEMPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMEFVCELLGVRDPSRLSRGLKPFDRRKLETALKGVNVEVTHRKSSRQ 420
            QTSQNAYDCITALDISLRHAPSMKLTCVGRSFFTP+MPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMM+FVCELLGVRDP +LSRG++P+DRRKLETALKGVNVEVTHRKS+RQ
Sbjct:  246 QTSQNAYDCITALDISLRHAPSMKLTCVGRSFFTPDMPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMDFVCELLGVRDPGQLSRGIRPYDRRKLETALKGVNVEVTHRKSNRQ 371          
BLAST of mRNA_L-elsbetiae_contig19097.5623.1 vs. uniprot
Match: A0A2S1PRW8_FUCSE (Putative argonaute n=1 Tax=Fucus serratus TaxID=87148 RepID=A0A2S1PRW8_FUCSE)

HSP 1 Score: 233 bits (594), Expect = 6.430e-69
Identity = 112/125 (89.60%), Postives = 122/125 (97.60%), Query Frame = 1
Query:   46 TSQNAYDCITALDISLRHAPSMKLTCVGRSFFTPEMPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMEFVCELLGVRDPSRLSRGLKPFDRRKLETALKGVNVEVTHRKSSRQ 420
            T+QNAYDCITALDISLRHAPSM LTCVGRSF++PEMPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMM+FVCELLG+RDP  LSRG++P+D+RKLETALKGVNVEVTHRKS+RQ
Sbjct:  256 TTQNAYDCITALDISLRHAPSMALTCVGRSFYSPEMPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMDFVCELLGIRDPGMLSRGIRPYDKRKLETALKGVNVEVTHRKSNRQ 380          
BLAST of mRNA_L-elsbetiae_contig19097.5623.1 vs. uniprot
Match: A0A397TJF0_9GLOM (Piwi domain-containing protein n=1 Tax=Glomus cerebriforme TaxID=658196 RepID=A0A397TJF0_9GLOM)

HSP 1 Score: 119 bits (299), Expect = 1.280e-28
Identity = 58/123 (47.15%), Postives = 79/123 (64.23%), Query Frame = 1
Query:   37 RQQTSQNAYDCITALDISLRHAPSMKLTCVGRSFFTPEMPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMEFVCELLGVRDPSRLSRGLKPFDRRKLETALKGVNVEVTHR 405
            ++Q + N    I ALD+ + H PSMK   VGRSFFTP+    +SGGAEVW GYYQS R TQ  + +NVD+SA  F    P+++ + +LLG R P  L RG+   D +K+E ++K + V VTHR
Sbjct:  123 KRQLTSNCLTAIMALDVLIHHEPSMKFVTVGRSFFTPQDSQVLSGGAEVWQGYYQSARPTQRKMMINVDLSATTFYERGPLLQMITKLLGRRTPDDLRRGVTSKDYQKVERSIKNLKVRVTHR 245          
BLAST of mRNA_L-elsbetiae_contig19097.5623.1 vs. uniprot
Match: A0A015JUG7_RHIIW (Uncharacterized protein n=8 Tax=Rhizophagus TaxID=1129544 RepID=A0A015JUG7_RHIIW)

HSP 1 Score: 119 bits (297), Expect = 2.380e-28
Identity = 59/130 (45.38%), Postives = 82/130 (63.08%), Query Frame = 1
Query:   16 LLVYLCIRQQTSQNAYDCITALDISLRHAPSMKLTCVGRSFFTPEMPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMEFVCELLGVRDPSRLSRGLKPFDRRKLETALKGVNVEVTHR 405
            L+++L  ++Q + N    I ALD+ + H PSM    VG SFFTP    PISGGAE+W GYYQS R TQ  + +NVD+SA AF    P++  + +LLG R P  L RG+   D +K+E ++K + + VTHR
Sbjct:  137 LVLFLGGKRQLTPNCLTAIMALDVIIHHQPSMDYVTVGHSFFTPRDSRPISGGAEIWQGYYQSARPTQGKMMINVDLSATAFYERGPLLLMITKLLGRRTPDDLRRGVSDKDYQKIERSIKNLKIRVTHR 266          
BLAST of mRNA_L-elsbetiae_contig19097.5623.1 vs. uniprot
Match: A0A2P4P2X8_RHIID (Uncharacterized protein n=1 Tax=Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) TaxID=747089 RepID=A0A2P4P2X8_RHIID)

HSP 1 Score: 119 bits (297), Expect = 2.520e-28
Identity = 59/130 (45.38%), Postives = 82/130 (63.08%), Query Frame = 1
Query:   16 LLVYLCIRQQTSQNAYDCITALDISLRHAPSMKLTCVGRSFFTPEMPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMEFVCELLGVRDPSRLSRGLKPFDRRKLETALKGVNVEVTHR 405
            L+++L  ++Q + N    I ALD+ + H PSM    VG SFFTP    PISGGAE+W GYYQS R TQ  + +NVD+SA AF    P++  + +LLG R P  L RG+   D +K+E ++K + + VTHR
Sbjct:  134 LVLFLGGKRQLTPNCLTAIMALDVIIHHQPSMDYVTVGHSFFTPRDSRPISGGAEIWQGYYQSARPTQGKMMINVDLSATAFYERGPLLLMITKLLGRRTPDDLRRGVSDKDYQKIERSIKNLKIRVTHR 263          
BLAST of mRNA_L-elsbetiae_contig19097.5623.1 vs. uniprot
Match: A0A835Z8Z7_9STRA (Argonaute 1 n=2 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z8Z7_9STRA)

HSP 1 Score: 117 bits (293), Expect = 8.290e-28
Identity = 59/129 (45.74%), Postives = 84/129 (65.12%), Query Frame = 1
Query:   34 IRQQTSQNAYDCITALDISLRHAPSMKLTCVGRSFFTPEMPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMEFVCELLGVRDPSRLSRGLKPF-DRRKLETALKGVNVEVTHRKSSR 417
            +R +  ++A D I ALD+ LR  P+MKL   GR+F+ P    PISGGAEVWLGYYQSLR  QAG+T N D++A AF++ MP++EF+C  L  RDP +L + L     +++   A+  + + V HR + R
Sbjct:  168 LRGERRESAVDVINALDVVLRTVPTMKLVSAGRNFYMPTSDYPISGGAEVWLGYYQSLRPGQAGVTFNADLAASAFLKPMPVLEFLCLQLNCRDPRQLQQALGEMRGQKQASKAIATLAITVNHRPTKR 296          
BLAST of mRNA_L-elsbetiae_contig19097.5623.1 vs. uniprot
Match: A0A6V7IJU4_9HYME (PAZ domain-containing protein (Fragment) n=1 Tax=Bracon brevicornis TaxID=1563983 RepID=A0A6V7IJU4_9HYME)

HSP 1 Score: 111 bits (278), Expect = 5.120e-27
Identity = 56/122 (45.90%), Postives = 81/122 (66.39%), Query Frame = 1
Query:   43 QTSQNAYDCITALDISLRHAPSMKLTCVGRSFF-TPE-MPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMEFVCELLGVRDPSRLSRGLKPFDRRKLETALKGVNVEVTH 402
            +T Q  YD I ALD+ +RH PSM  T VGRSFF TP+    P+ GG EVW G++QS+R +Q  + LN+D+SA AF ++ P++EF+CE+L +RD +   + L    R K    +KG+ +E+TH
Sbjct:   34 RTRQIPYDAILALDVVMRHLPSMTYTPVGRSFFSTPDGYYHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRDVNEQRKPLTDSQRVKFTKEIKGLKIEITH 155          
BLAST of mRNA_L-elsbetiae_contig19097.5623.1 vs. uniprot
Match: A0A7S3UXA3_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UXA3_HETAK)

HSP 1 Score: 115 bits (287), Expect = 5.320e-27
Identity = 66/128 (51.56%), Postives = 85/128 (66.41%), Query Frame = 1
Query:   46 TSQNA-YDCITALDISLRHAPSMKLTCVGRSFFTPEMPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMEF---VCELLGVRDPSRLSRGLKPFDRRKLETALKGVNVEVTHRKSSR 417
            TS N   DC TALDI LR+ PS+++T VG SFF+ E    +SGG E+W G+YQSLRATQ+GLTLNVD SA AFVR  P++E+   VC    V D   L RG+ P + R L++ L  + V V HR ++R
Sbjct:  160 TSSNVPLDCFTALDIVLRNMPSVRMTSVGASFFSNENAFQMSGGTELWFGWYQSLRATQSGLTLNVDTSATAFVRPQPVLEYAAHVCRKRSVDD--LLHRGIGPREHRILKSELNWLMVSVNHRDNAR 285          
BLAST of mRNA_L-elsbetiae_contig19097.5623.1 vs. uniprot
Match: A0A026VZA9_OOCBI (Protein argonaute-4 n=1 Tax=Ooceraea biroi TaxID=2015173 RepID=A0A026VZA9_OOCBI)

HSP 1 Score: 111 bits (277), Expect = 9.480e-27
Identity = 56/122 (45.90%), Postives = 81/122 (66.39%), Query Frame = 1
Query:   43 QTSQNAYDCITALDISLRHAPSMKLTCVGRSFF-TPE-MPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMEFVCELLGVRDPSRLSRGLKPFDRRKLETALKGVNVEVTH 402
            +T Q  YD I ALD+ +RH PSM  T VGRSFF TP+    P+ GG EVW G++QS+R +Q  + LN+D+SA AF ++ P++EF+CE+L +RD +   + L    R K    +KG+ +E+TH
Sbjct:  187 RTRQIPYDAILALDVVMRHLPSMTYTPVGRSFFSTPDGYYHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRDINEQRKPLTDSQRVKFTKEIKGLKIEITH 308          
BLAST of mRNA_L-elsbetiae_contig19097.5623.1 vs. uniprot
Match: A0A015ILK1_RHIIW (Uncharacterized protein n=11 Tax=Glomeraceae TaxID=36751 RepID=A0A015ILK1_RHIIW)

HSP 1 Score: 114 bits (284), Expect = 1.340e-26
Identity = 58/117 (49.57%), Postives = 76/117 (64.96%), Query Frame = 1
Query:   64 DCITA---LDISLRHAPSMKLTCVGRSFFTPEMPSPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMEFVCELLGVRDPSRLSRGLKPFDRRKLETALKGVNVEVTHR 405
            +C+TA   LD+ +RH PSM+   VGRSFFT     P+ GGAEVW GYYQS R  Q  + +NVD+SA AF  S P+++ V ++LG R+P  L RG    DR K+E  LK + + VTHR
Sbjct:  147 NCLTATMALDVLIRHLPSMRHVTVGRSFFTNAGAEPLFGGAEVWQGYYQSARPAQEKMLINVDLSATAFYESGPLIQMVTKILGRRNPDDLRRGFSDKDRIKVEKTLKNLKIRVTHR 263          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig19097.5623.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FQK3_ECTSI2.410e-7293.65Argonaute 1 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7... [more]
A0A2S1PRW8_FUCSE6.430e-6989.60Putative argonaute n=1 Tax=Fucus serratus TaxID=87... [more]
A0A397TJF0_9GLOM1.280e-2847.15Piwi domain-containing protein n=1 Tax=Glomus cere... [more]
A0A015JUG7_RHIIW2.380e-2845.38Uncharacterized protein n=8 Tax=Rhizophagus TaxID=... [more]
A0A2P4P2X8_RHIID2.520e-2845.38Uncharacterized protein n=1 Tax=Rhizophagus irregu... [more]
A0A835Z8Z7_9STRA8.290e-2845.74Argonaute 1 n=2 Tax=Tribonema minus TaxID=303371 R... [more]
A0A6V7IJU4_9HYME5.120e-2745.90PAZ domain-containing protein (Fragment) n=1 Tax=B... [more]
A0A7S3UXA3_HETAK5.320e-2751.56Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
A0A026VZA9_OOCBI9.480e-2745.90Protein argonaute-4 n=1 Tax=Ooceraea biroi TaxID=2... [more]
A0A015ILK1_RHIIW1.340e-2649.57Uncharacterized protein n=11 Tax=Glomeraceae TaxID... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig19097contigL-elsbetiae_contig19097:141..1434 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score244.2
Seed ortholog evalue3.6e-62
Seed eggNOG ortholog2880.D7FQK3
KEGG koko:K11593
Hectar predicted targeting categoryother localisation
EggNOG free text desc.gene silencing by RNA
EggNOG OGsKOG1041@1,KOG1041@2759
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03019,ko03036
Ec32 ortholog descriptionArgonaute 1
Ec32 orthologEc-01_009010.1
Exons2
Model size426
Cds size420
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig19097.5623.1prot_L-elsbetiae_contig19097.5623.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig19097 147..1434 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815793.4549541-UTR-L-elsbetiae_contig19097:140..1461622815793.4549541-UTR-L-elsbetiae_contig19097:140..146Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig19097 141..146 +
1691683106.6340318-UTR-L-elsbetiae_contig19097:140..1461691683106.6340318-UTR-L-elsbetiae_contig19097:140..146Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig19097 141..146 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815793.4672937-CDS-L-elsbetiae_contig19097:146..4191622815793.4672937-CDS-L-elsbetiae_contig19097:146..419Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig19097 147..419 +
1691683106.6474905-CDS-L-elsbetiae_contig19097:146..4191691683106.6474905-CDS-L-elsbetiae_contig19097:146..419Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig19097 147..419 +
1622815793.4815514-CDS-L-elsbetiae_contig19097:1287..14341622815793.4815514-CDS-L-elsbetiae_contig19097:1287..1434Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig19097 1288..1434 +
1691683106.6570554-CDS-L-elsbetiae_contig19097:1287..14341691683106.6570554-CDS-L-elsbetiae_contig19097:1287..1434Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig19097 1288..1434 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig19097.5623.1

>prot_L-elsbetiae_contig19097.5623.1 ID=prot_L-elsbetiae_contig19097.5623.1|Name=mRNA_L-elsbetiae_contig19097.5623.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=140bp
MYVLLVYLCIRQQTSQNAYDCITALDISLRHAPSMKLTCVGRSFFTPEMP
SPISGGAEVWLGYYQSLRATQAGLTLNVDMSAMAFVRSMPMMEFVCELLG
VRDPSRLSRGLKPFDRRKLETALKGVNVEVTHRKSSRQV*
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mRNA from alignment at L-elsbetiae_contig19097:141..1434+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig19097.5623.1 ID=mRNA_L-elsbetiae_contig19097.5623.1|Name=mRNA_L-elsbetiae_contig19097.5623.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1294bp|location=Sequence derived from alignment at L-elsbetiae_contig19097:141..1434+ (Laminarionema elsbetiae ELsaHSoW15)
GTGCATATGTATGTGCTCCTCGTTTATTTATGTATTCGGCAGCAAACCAG CCAGAACGCGTACGACTGCATCACGGCACTCGACATCTCGCTCCGGCACG CGCCGTCCATGAAGCTCACGTGCGTCGGGCGCAGCTTCTTCACGCCCGAG ATGCCGTCGCCCATCTCCGGGGGCGCGGAGGTGTGGCTCGGCTACTACCA GTCGCTGAGGGCCACGCAGGCAGGCCTGACGCTCAACGTGGACATGAGCG CGATGGCCTTCGTGCGGAGCATGCCCATGGTGAGGAGGGCGGGGGGCAAG GGGGGAAAGGATGGTGTCAACTTCGGGAAGAGAAGCGATGAAAATGCGGG AATATGTATCTGTATCTGTGTATTTTGACATACATATGTACATACATACG AGAAGGAAAGGTAGATGTCTTGTTTTTGTGGGTGGGTGGGTGGGTGGGTG TGTGCGCGCGCCCGCGCGGCGCTGAGTTCGTAAGTTAAGGATACTGTAGG ACGGAAATGAGAGGCACGCCGTTCTAGTTTGAGATGTGTGGAGGTTGCTC CCTTTCCTATAGCGACGGAGACCAGGCAGATGGGGACTGATCGAGGATGG ATGCGATCGACGCACGAGGGGTGTTTCGTGCGCAGAGAAAAAAACACGCG CAGGTTGCCGTACGAAATTAGCAGGGTGTGTGTTTTGCCAAGGGTAGACA GGGAAAAAAAGCAAGTTGTCCTGTTACCACGGGAATCGACCGCGTCAGTG TTGGATTTTTTAATTATTACGGACTCATGGGGGTACTGGATAGAGAGATC GGAACGGTTTTAAATGTGAAGACAACAACAACCATAGGAGGAGGGCGTTT GATATGATGAGCGCGATGGCGCAGCTCAGAGAGGCGTGCAGCGTCCTATG CCGTGGTGAATTGCTCTCTCTTTGTCGACTGCGATAGCTTGGTTCACTAC GCGAACACTCGCAATGCTTTGTGGACCGATGCGAAGCTTTCAACCTTGTG ATACAAAAATACAGTAAACGGTACTGTAACGCGAATGTTTGCCGCCCCCC CCCGTGTGCTCTCGATCCCCTTTCGATCTTGCTTTTTCTCGCCGTGGCCA ATACCGCACTACCTTTCTGTCCATCGGTCCCCGTCTTTCTCCGCCAGATG GAGTTCGTGTGCGAGCTTCTCGGTGTCCGGGACCCCAGTCGACTGAGCCG CGGTTTGAAGCCGTTCGATCGCCGCAAGCTGGAGACCGCCCTCAAGGGGG TCAACGTCGAGGTCACGCACCGAAAGTCCAGCCGGCAGGTATGA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig19097:141..1434+

>mRNA_L-elsbetiae_contig19097.5623.1 ID=mRNA_L-elsbetiae_contig19097.5623.1|Name=mRNA_L-elsbetiae_contig19097.5623.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=840bp|location=Sequence derived from alignment at L-elsbetiae_contig19097:141..1434+ (Laminarionema elsbetiae ELsaHSoW15)
ATGTATGTGCTCCTCGTTTATTTATGTATTCGGCAGCAAACCAGCCAGAA
CGCGTACGACTGCATCACGGCACTCGACATCTCGCTCCGGCACGCGCCGT
CCATGAAGCTCACGTGCGTCGGGCGCAGCTTCTTCACGCCCGAGATGCCG
TCGCCCATCTCCGGGGGCGCGGAGGTGTGGCTCGGCTACTACCAGTCGCT
GAGGGCCACGCAGGCAGGCCTGACGCTCAACGTGGACATGAGCGCGATGG
CCTTCGTGCGGAGCATGCCCATGATGTATGTGCTCCTCGTTTATTTATGT
ATTCGGCAGCAAACCAGCCAGAACGCGTACGACTGCATCACGGCACTCGA
CATCTCGCTCCGGCACGCGCCGTCCATGAAGCTCACGTGCGTCGGGCGCA
GCTTCTTCACGCCCGAGATGCCGTCGCCCATCTCCGGGGGCGCGGAGGTG
TGGCTCGGCTACTACCAGTCGCTGAGGGCCACGCAGGCAGGCCTGACGCT
CAACGTGGACATGAGCGCGATGGCCTTCGTGCGGAGCATGCCCATGATGG
AGTTCGTGTGCGAGCTTCTCGGTGTCCGGGACCCCAGTCGACTGAGCCGC
GGTTTGAAGCCGTTCGATCGCCGCAAGCTGGAGACCGCCCTCAAGGGGGT
CAACGTCGAGGTCACGCACCGAAAGTCCAGCCGGCAGGTATGAATGGAGT
TCGTGTGCGAGCTTCTCGGTGTCCGGGACCCCAGTCGACTGAGCCGCGGT
TTGAAGCCGTTCGATCGCCGCAAGCTGGAGACCGCCCTCAAGGGGGTCAA
CGTCGAGGTCACGCACCGAAAGTCCAGCCGGCAGGTATGA
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