mRNA_L-elsbetiae_contig18190.5249.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig18190.5249.1
Unique NamemRNA_L-elsbetiae_contig18190.5249.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig18190.5249.1 vs. uniprot
Match: D8LJL4_ECTSI (Ribonuclease Z n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LJL4_ECTSI)

HSP 1 Score: 288 bits (738), Expect = 6.480e-88
Identity = 158/222 (71.17%), Postives = 174/222 (78.38%), Query Frame = 1
Query:   10 QEYGNIDPAVRGGYDFLDASLMLSGPNAPRPSPHVAAAAARALARAGVLRATSVRVRHCAHAYALVLTVASTAXXXXXXXXXXXXXAH----TVXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGASKDWKLVYSGDCRPSETLVKEGKGAAVLIHEATFDETKQQEAYDKQHCTMPEALSMGQRMGAQRIILTHFSQRYPHMPTALTGGLGTNACLAFDNMR 663
            +EYG++DP VRGGYDFLDASLM +G NAPRP PH+AAAAARALARAGVLRATSVRVRHCAHAYALVLTVASTAXXXXXXXX     A     T                             EGA KDWKLVYSGDCRPSE+LV+EGKGAAVLIHEATFDETKQQEA+DK+HCTM EA+++GQRMGA+R+ILTHFSQRYPHMP++L GGLGT  CLAFDNMR
Sbjct:  724 KEYGDVDPTVRGGYDFLDASLMQAGDNAPRPPPHIAAAAARALARAGVLRATSVRVRHCAHAYALVLTVASTAXXXXXXXXRAVFSAGPSVATNAVFGGRGGGVADSRATAQEGGDGSGVPGEGAGKDWKLVYSGDCRPSESLVREGKGAAVLIHEATFDETKQQEAFDKRHCTMQEAMAVGQRMGAERVILTHFSQRYPHMPSSLAGGLGTKVCLAFDNMR 945          
BLAST of mRNA_L-elsbetiae_contig18190.5249.1 vs. uniprot
Match: A0A6H5LFG5_9PHAE (Ribonuclease Z n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LFG5_9PHAE)

HSP 1 Score: 288 bits (738), Expect = 2.580e-87
Identity = 160/226 (70.80%), Postives = 176/226 (77.88%), Query Frame = 1
Query:    1 MQTQEYGNIDPAVRGGYDFLDASLMLSGPNAPRPSPHVAAAAARALARAGVLRATSVRVRHCAHAYALVLTVASTAXXXXXXXXXXXXXAH-----TVXXXXXXXXXXXXXXXXXXXXXXXXXXXXEGASKDWKLVYSGDCRPSETLVKEGKGAAVLIHEATFDETKQQEAYDKQHCTMPEALSMGQRMGAQRIILTHFSQRYPHMPTALTGGLGTNACLAFDNMR 663
            + ++EYG++DP VRGGYDFLDASLM +G NAPRP PH+AAAAARALARAGVLRATSVRVRHCAHAYALVLTVAST XXXXXXXXX    A             X                      EGA KDWKLVYSGDCRPSE LV+EGKGAAVLIHEATFDETKQQEA+DK+HCTM EAL++GQRMGA+R+ILTHFSQRYPHMP++L GGLGTN CLAFDNMR
Sbjct:  830 VPSKEYGDVDPTVRGGYDFLDASLMQAGDNAPRPPPHIAAAAARALARAGVLRATSVRVRHCAHAYALVLTVASTXXXXXXXXXXAVFSASPSVAANAVFGGRXGGGGVADSQARQGGDDGSGVPGEGAGKDWKLVYSGDCRPSEPLVREGKGAAVLIHEATFDETKQQEAFDKRHCTMQEALTVGQRMGAKRVILTHFSQRYPHMPSSLAGGLGTNVCLAFDNMR 1055          
BLAST of mRNA_L-elsbetiae_contig18190.5249.1 vs. uniprot
Match: A0A836CGF5_9STRA (Ribonuclease Z n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CGF5_9STRA)

HSP 1 Score: 117 bits (293), Expect = 1.500e-26
Identity = 59/96 (61.46%), Postives = 68/96 (70.83%), Query Frame = 1
Query:  382 WKLVYSGDCRPSETLVKEGKGAAVLIHEATFDETKQQEAYDKQHCTMPEALSMGQRMGAQRIILTHFSQRYPHMPTALTG--GLGTNACLAFDNMR 663
            WKLVYSGD RP E LV+ G GA VLIHEATF++ KQQ+A DK+H T+ EAL + QRMGA R ILTHFSQRYP +P       G    A +AFD MR
Sbjct:  735 WKLVYSGDTRPCEALVQAGMGATVLIHEATFEDAKQQDAVDKKHSTVSEALDVAQRMGAHRTILTHFSQRYPKLPVLPPSCYGADATALIAFDYMR 830          
BLAST of mRNA_L-elsbetiae_contig18190.5249.1 vs. uniprot
Match: A0A7S4D8B2_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4D8B2_HETAK)

HSP 1 Score: 107 bits (267), Expect = 5.170e-26
Identity = 55/94 (58.51%), Postives = 64/94 (68.09%), Query Frame = 1
Query:  382 WKLVYSGDCRPSETLVKEGKGAAVLIHEATFDETKQQEAYDKQHCTMPEALSMGQRMGAQRIILTHFSQRYPHMPTALTGGLGTNACLAFDNMR 663
            WKLVYSGDCRPS+ L + G+GA VLIHEATF+E K  EA  K+HCT+ EAL + + MGA R  LTHFSQRYP  P  L         +AFD MR
Sbjct:    1 WKLVYSGDCRPSDRLAELGRGATVLIHEATFEEDKADEAEKKKHCTVGEALGVARNMGAFRSFLTHFSQRYPKFPV-LPEEDSFRTIIAFDFMR 93          
BLAST of mRNA_L-elsbetiae_contig18190.5249.1 vs. uniprot
Match: A0A7S2C1Q9_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2C1Q9_9STRA)

HSP 1 Score: 102 bits (254), Expect = 8.180e-24
Identity = 50/91 (54.95%), Postives = 66/91 (72.53%), Query Frame = 1
Query:  382 WKLVYSGDCRPSETLVKEGKGAAVLIHEATFDETKQQEAYDKQHCTMPEALSMGQRMGAQRIILTHFSQRYPHMPTALTGGLGTNACLAFD 654
            WK+VYSGDCRPS+ LV+ G GA VLIHEATF+++ Q++A  K+H T+ EA+ +  RMGA R ILTHFSQRYP + + L   +     +AFD
Sbjct:    3 WKVVYSGDCRPSDALVRAGIGATVLIHEATFEDSMQEDAVAKKHSTVSEAIDVACRMGAHRTILTHFSQRYPAL-SPLPEDVSGRVLVAFD 92          
BLAST of mRNA_L-elsbetiae_contig18190.5249.1 vs. uniprot
Match: A0A1L7WP19_9HELO (Ribonuclease Z n=1 Tax=Phialocephala subalpina TaxID=576137 RepID=A0A1L7WP19_9HELO)

HSP 1 Score: 109 bits (272), Expect = 9.360e-24
Identity = 55/96 (57.29%), Postives = 70/96 (72.92%), Query Frame = 1
Query:  382 WKLVYSGDCRPSETLVKEGKGAAVLIHEATFDETKQQEAYDKQHCTMPEALSMGQRMGAQRIILTHFSQRYPHMPTALT-GGLGTNACLAFDNMRC 666
            +K+ YSGDCRPSE  V+ GKGA +LIHEATFD+  Q +AY K+H T  EAL +G++M A+RI+LTHFSQRY  +P   + GG    A +AFD MRC
Sbjct:  712 FKVAYSGDCRPSEDFVQIGKGATLLIHEATFDDELQGDAYAKKHSTTSEALEVGRQMNARRILLTHFSQRYQKIPVMDSHGGKDQVAIVAFDYMRC 807          
BLAST of mRNA_L-elsbetiae_contig18190.5249.1 vs. uniprot
Match: A0A1E1K6D4_9HELO (Ribonuclease Z n=3 Tax=Rhynchosporium TaxID=38037 RepID=A0A1E1K6D4_9HELO)

HSP 1 Score: 108 bits (270), Expect = 1.760e-23
Identity = 53/95 (55.79%), Postives = 67/95 (70.53%), Query Frame = 1
Query:  382 WKLVYSGDCRPSETLVKEGKGAAVLIHEATFDETKQQEAYDKQHCTMPEALSMGQRMGAQRIILTHFSQRYPHMPTALTGGLGTNACLAFDNMRC 666
            +K+ YSGDCRPSE  V+ GK A +LIHEATFD+  + +A  K+H T  EAL +G+RM A+RI+LTHFSQRY  +P   T G    A +AFD MRC
Sbjct:  787 FKVAYSGDCRPSERFVEIGKDATLLIHEATFDDELRSDAIAKKHSTTSEALGVGERMNARRILLTHFSQRYQKIPVMKTEGKDQVAIVAFDYMRC 881          
BLAST of mRNA_L-elsbetiae_contig18190.5249.1 vs. uniprot
Match: A0A4T0W987_9PEZI (Ribonuclease Z n=1 Tax=Colletotrichum higginsianum TaxID=80884 RepID=A0A4T0W987_9PEZI)

HSP 1 Score: 108 bits (269), Expect = 2.350e-23
Identity = 56/98 (57.14%), Postives = 70/98 (71.43%), Query Frame = 1
Query:  385 KLVYSGDCRPSETLVKEGKGAAVLIHEATFDETKQQEAYDKQHCTMPEALSMGQRMGAQRIILTHFSQRYPHMP-----TALTGGLGTNACLAFDNMR 663
            K+ YSGDCRPS+ LV+ GKGA +LIHE+TFD+ KQ +A  K+H TM EAL +G RMGA+R++LTHFSQRY  +P     T  TG   T   +AFD MR
Sbjct:  716 KIAYSGDCRPSDDLVEAGKGATLLIHESTFDDDKQGDALAKKHSTMSEALDVGYRMGARRVLLTHFSQRYAKIPLVEKRTTETGADQT-VLMAFDQMR 812          
BLAST of mRNA_L-elsbetiae_contig18190.5249.1 vs. uniprot
Match: H1V291_COLHI (Ribonuclease Z n=2 Tax=Colletotrichum higginsianum (strain IMI 349063) TaxID=759273 RepID=H1V291_COLHI)

HSP 1 Score: 108 bits (269), Expect = 2.470e-23
Identity = 56/98 (57.14%), Postives = 70/98 (71.43%), Query Frame = 1
Query:  385 KLVYSGDCRPSETLVKEGKGAAVLIHEATFDETKQQEAYDKQHCTMPEALSMGQRMGAQRIILTHFSQRYPHMP-----TALTGGLGTNACLAFDNMR 663
            K+ YSGDCRPS+ LV+ GKGA +LIHE+TFD+ KQ +A  K+H TM EAL +G RMGA+R++LTHFSQRY  +P     T  TG   T   +AFD MR
Sbjct:  939 KIAYSGDCRPSDDLVEAGKGATLLIHESTFDDDKQGDALAKKHSTMSEALDVGYRMGARRVLLTHFSQRYAKIPLVEKRTTETGADQT-VLMAFDQMR 1035          
BLAST of mRNA_L-elsbetiae_contig18190.5249.1 vs. uniprot
Match: A0A5Q4BD95_9PEZI (Ribonuclease Z n=1 Tax=Colletotrichum shisoi TaxID=2078593 RepID=A0A5Q4BD95_9PEZI)

HSP 1 Score: 108 bits (269), Expect = 2.510e-23
Identity = 56/98 (57.14%), Postives = 70/98 (71.43%), Query Frame = 1
Query:  385 KLVYSGDCRPSETLVKEGKGAAVLIHEATFDETKQQEAYDKQHCTMPEALSMGQRMGAQRIILTHFSQRYPHMP-----TALTGGLGTNACLAFDNMR 663
            K+ YSGDCRPS+ LV+ GKGA +LIHE+TFD+ KQ +A  K+H TM EAL +G RMGA+R++LTHFSQRY  +P     T  TG   T   +AFD MR
Sbjct: 1066 KIAYSGDCRPSDDLVEAGKGATLLIHESTFDDDKQGDALAKKHSTMSEALDVGYRMGARRVLLTHFSQRYAKIPLVEKRTTETGADQT-VLMAFDQMR 1162          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig18190.5249.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LJL4_ECTSI6.480e-8871.17Ribonuclease Z n=1 Tax=Ectocarpus siliculosus TaxI... [more]
A0A6H5LFG5_9PHAE2.580e-8770.80Ribonuclease Z n=1 Tax=Ectocarpus sp. CCAP 1310/34... [more]
A0A836CGF5_9STRA1.500e-2661.46Ribonuclease Z n=1 Tax=Tribonema minus TaxID=30337... [more]
A0A7S4D8B2_HETAK5.170e-2658.51Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A7S2C1Q9_9STRA8.180e-2454.95Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A1L7WP19_9HELO9.360e-2457.29Ribonuclease Z n=1 Tax=Phialocephala subalpina Tax... [more]
A0A1E1K6D4_9HELO1.760e-2355.79Ribonuclease Z n=3 Tax=Rhynchosporium TaxID=38037 ... [more]
A0A4T0W987_9PEZI2.350e-2357.14Ribonuclease Z n=1 Tax=Colletotrichum higginsianum... [more]
H1V291_COLHI2.470e-2357.14Ribonuclease Z n=2 Tax=Colletotrichum higginsianum... [more]
A0A5Q4BD95_9PEZI2.510e-2357.14Ribonuclease Z n=1 Tax=Colletotrichum shisoi TaxID... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig18190contigL-elsbetiae_contig18190:1961..2998 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score288.1
Seed ortholog evalue3.6e-75
Seed eggNOG ortholog2880.D8LJL4
KEGG koko:K00784
KEGG Pathwayko03013,map03013
Hectar predicted targeting categoryother localisation
EggNOG free text desc.3'-tRNA processing endoribonuclease activity
EggNOG OGsCOG1234@1,KOG2121@2759
EC3.1.26.11
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03016
Exons2
Model size687
Cds size687
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815758.1015277-CDS-L-elsbetiae_contig18190:1960..21551622815758.1015277-CDS-L-elsbetiae_contig18190:1960..2155Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig18190 1961..2155 -
1691683083.3946085-CDS-L-elsbetiae_contig18190:1960..21551691683083.3946085-CDS-L-elsbetiae_contig18190:1960..2155Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig18190 1961..2155 -
1622815758.1143494-CDS-L-elsbetiae_contig18190:2506..29981622815758.1143494-CDS-L-elsbetiae_contig18190:2506..2998Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig18190 2507..2998 -
1691683083.4076972-CDS-L-elsbetiae_contig18190:2506..29981691683083.4076972-CDS-L-elsbetiae_contig18190:2506..2998Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig18190 2507..2998 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig18190.5249.1prot_L-elsbetiae_contig18190.5249.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig18190 1961..2998 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig18190.5249.1

>prot_L-elsbetiae_contig18190.5249.1 ID=prot_L-elsbetiae_contig18190.5249.1|Name=mRNA_L-elsbetiae_contig18190.5249.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=229bp
MQTQEYGNIDPAVRGGYDFLDASLMLSGPNAPRPSPHVAAAAARALARAG
VLRATSVRVRHCAHAYALVLTVASTAARAAAAAAASALSAHTVVSGGGGG
GGGGLPGGGGGVCGGGSAGGGEGASKDWKLVYSGDCRPSETLVKEGKGAA
VLIHEATFDETKQQEAYDKQHCTMPEALSMGQRMGAQRIILTHFSQRYPH
MPTALTGGLGTNACLAFDNMRCVQYVHM*
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mRNA from alignment at L-elsbetiae_contig18190:1961..2998-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig18190.5249.1 ID=mRNA_L-elsbetiae_contig18190.5249.1|Name=mRNA_L-elsbetiae_contig18190.5249.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1038bp|location=Sequence derived from alignment at L-elsbetiae_contig18190:1961..2998- (Laminarionema elsbetiae ELsaHSoW15)
ATGCAAACGCAGGAGTACGGCAACATCGACCCCGCGGTGCGGGGCGGCTA CGACTTCCTCGACGCCAGCCTGATGCTGTCCGGGCCCAACGCCCCCCGGC CCTCGCCCCACGTCGCCGCCGCGGCCGCCCGCGCGCTGGCCAGGGCCGGC GTCCTCCGGGCGACGAGCGTCCGCGTGCGCCACTGTGCGCACGCGTACGC GCTCGTCCTCACCGTCGCGTCCACGGCCGCGCGGGCGGCCGCCGCCGCCG CGGCCAGCGCTCTGTCGGCCCACACCGTTGTGTCGGGCGGTGGTGGTGGT GGTGGTGGTGGTTTGCCGGGAGGGGGGGGCGGCGTGTGCGGGGGGGGCTC CGCGGGCGGCGGGGAGGGGGCGAGCAAGGACTGGAAGCTGGTGTACAGCG GCGACTGCCGGCCGAGCGAGACGCTGGTGAAGGAGGGGAAGGGCGCGGCC GTGCTGATCCACGAGGCCACGTTCGACGAGACCAAGCAGCAGGTCTGTTT GTTTGTTTGGCTGTTTGTGTGGCTGTTTTGTTGAGGTGCTTTTGATTGTG TGTGTGTGTGTGCTTTATTTTACTCTTTTTTTCTGGCTGACGGGGCTGTC GTGGCTCGGTGGTGTGATGGTGGCGTATGATGAAAGTGTTTGGCTGCTGG CGTGAGATGGCGGTGTCCGATGCCTTTTTCGATGTTTTATCGAAATCTTT GGTGCTCTTTGGTGTTTGTTTAGTCCAATGGTTGGTCTTGTTGCACGCTC ATCATTGAAGGTGGCCGAACAGCTAAAAGAAACAGCATTCGCCCTGAACA TAAATGTGTCTGTCCCCTCCCTATTATTTGGTTCACTCGCCAGGAGGCCT ACGATAAGCAGCACTGCACGATGCCCGAGGCCCTCTCGATGGGGCAGCGC ATGGGGGCCCAGCGGATTATCCTGACCCACTTCAGCCAGCGGTACCCCCA CATGCCGACGGCCCTCACCGGGGGGCTTGGCACCAATGCCTGCCTCGCGT TCGACAACATGCGGTGCGTTCAGTACGTACATATGTAA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig18190:1961..2998-

>mRNA_L-elsbetiae_contig18190.5249.1 ID=mRNA_L-elsbetiae_contig18190.5249.1|Name=mRNA_L-elsbetiae_contig18190.5249.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=1374bp|location=Sequence derived from alignment at L-elsbetiae_contig18190:1961..2998- (Laminarionema elsbetiae ELsaHSoW15)
ATGCAAACGCAGGAGTACGGCAACATCGACCCCGCGGTGCGGGGCGGCTA
CGACTTCCTCGACGCCAGCCTGATGCTGTCCGGGCCCAACGCCCCCCGGC
CCTCGCCCCACGTCGCCGCCGCGGCCGCCCGCGCGCTGGCCAGGGCCGGC
GTCCTCCGGGCGACGAGCGTCCGCGTGCGCCACTGTGCGCACGCGTACGC
GCTCGTCCTCACCGTCGCGTCCACGGCCGCGCGGGCGGCCGCCGCCGCCG
CGGCCAGCGCTCTGTCGGCCCACACCGTTGTGTCGGGCGGTGGTGGTGGT
GGTGGTGGTGGTTTGCCGGGAGGGGGGGGCGGCGTGTGCGGGGGGGGCTC
CGCGGGCGGCGGGGAGGGGGCGAGCAAGGACTGGAAGCTGGTGTACAGCG
GCGACTGCCGGCCGAGCGAGACGCTGGTGAAGGAGGGGAAGGGCGCGGCC
GTGCTGATCCACGAGGCCACGTTCGACGAGACCAAGCAGCAGATGCAAAC
GCAGGAGTACGGCAACATCGACCCCGCGGTGCGGGGCGGCTACGACTTCC
TCGACGCCAGCCTGATGCTGTCCGGGCCCAACGCCCCCCGGCCCTCGCCC
CACGTCGCCGCCGCGGCCGCCCGCGCGCTGGCCAGGGCCGGCGTCCTCCG
GGCGACGAGCGTCCGCGTGCGCCACTGTGCGCACGCGTACGCGCTCGTCC
TCACCGTCGCGTCCACGGCCGCGCGGGCGGCCGCCGCCGCCGCGGCCAGC
GCTCTGTCGGCCCACACCGTTGTGTCGGGCGGTGGTGGTGGTGGTGGTGG
TGGTTTGCCGGGAGGGGGGGGCGGCGTGTGCGGGGGGGGCTCCGCGGGCG
GCGGGGAGGGGGCGAGCAAGGACTGGAAGCTGGTGTACAGCGGCGACTGC
CGGCCGAGCGAGACGCTGGTGAAGGAGGGGAAGGGCGCGGCCGTGCTGAT
CCACGAGGCCACGTTCGACGAGACCAAGCAGCAGGAGGCCTACGATAAGC
AGCACTGCACGATGCCCGAGGCCCTCTCGATGGGGCAGCGCATGGGGGCC
CAGCGGATTATCCTGACCCACTTCAGCCAGCGGTACCCCCACATGCCGAC
GGCCCTCACCGGGGGGCTTGGCACCAATGCCTGCCTCGCGTTCGACAACA
TGCGGTGCGTTCAGTACGTACATATGTAAGAGGCCTACGATAAGCAGCAC
TGCACGATGCCCGAGGCCCTCTCGATGGGGCAGCGCATGGGGGCCCAGCG
GATTATCCTGACCCACTTCAGCCAGCGGTACCCCCACATGCCGACGGCCC
TCACCGGGGGGCTTGGCACCAATGCCTGCCTCGCGTTCGACAACATGCGG
TGCGTTCAGTACGTACATATGTAA
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