mRNA_L-elsbetiae_contig16939.4651.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig16939.4651.1
Unique NamemRNA_L-elsbetiae_contig16939.4651.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig16939.4651.1 vs. uniprot
Match: D7FXU0_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXU0_ECTSI)

HSP 1 Score: 186 bits (472), Expect = 4.720e-57
Identity = 100/157 (63.69%), Postives = 111/157 (70.70%), Query Frame = 1
Query:    1 RPLLTKSLTAAVISVLGEIAASALRR--------------------------------LLRRTAAFAFFGLVLNGPVFHWWYGALERAAARRRKPGEPPGGTGDIAFKVALDRFLLTPPYLAITLTSLRLLQGLGAKRSLAETRALYRGALVTNWKV 375
            RPLLTKS+T+A++S LGE+  SALR                                 LLRRTAAFA FGL++NGPVFHWWYGALE AAARRRK GEPPGG GDI FKVA+DRFL+TPPYLAITL SLRLLQGLGAKRS+ ET ALYRG L TNWK+
Sbjct:   56 RPLLTKSVTSALVSALGELMGSALRSSPPXXXXXXXXXXXXXXXXXXXXXXXXXAAPGLLRRTAAFAIFGLLVNGPVFHWWYGALEGAAARRRKAGEPPGGAGDITFKVAVDRFLMTPPYLAITLASLRLLQGLGAKRSIGETSALYRGVLFTNWKI 212          
BLAST of mRNA_L-elsbetiae_contig16939.4651.1 vs. uniprot
Match: A0A6H5L3A8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L3A8_9PHAE)

HSP 1 Score: 185 bits (469), Expect = 3.140e-56
Identity = 101/156 (64.74%), Postives = 111/156 (71.15%), Query Frame = 1
Query:    1 RPLLTKSLTAAVISVLGEIAASALRR--------------------------------LLRRTAAFAFFGLVLNGPVFHWWYGALERAAARRRKPGEPPGGTGDIAFKVALDRFLLTPPYLAITLTSLRLLQGLGAKRSLAETRALYRGALVTNWK 372
            RPLLTKS+T+A++S LGE+A SALR                                 LLRRTAAFA FGL++NGPVFHWWYGALERAAARRRK GE PGG GDI FKVA+DRFL+TPPYLAITL SLRLLQGLGAKRS+ ET ALYRG L TNWK
Sbjct:   50 RPLLTKSVTSALVSALGELAGSALRSSSPPPLPVGAGTALAVKGRAGAGQRTGTAAPGLLRRTAAFAIFGLLVNGPVFHWWYGALERAAARRRKAGELPGGAGDITFKVAVDRFLMTPPYLAITLASLRLLQGLGAKRSIDETSALYRGVLFTNWK 205          
BLAST of mRNA_L-elsbetiae_contig16939.4651.1 vs. uniprot
Match: A0A836CQ06_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CQ06_9STRA)

HSP 1 Score: 110 bits (275), Expect = 4.620e-28
Identity = 63/131 (48.09%), Postives = 83/131 (63.36%), Query Frame = 1
Query:    4 PLLTKSLTAAVISVLGEIAASALRRL--------LRRTAAFAFFGLVLNGPVFHWWYGALERAAARRRKPGEPPGGTGDIAFKVALDRFLLTPPYLAITLTSLRLLQGLGAKRSLAETRALYRGALVTNWK 372
            PL TK++TAA +  +GE+  + LR+         L+R  AF  FGLV+NGPVFH+WYG LE+ A   RK  +  GG   I FK+ LDR LLTPPYLA+TL  LR LQ      ++  +R +Y+ AL+TNWK
Sbjct:   43 PLKTKAITAACMGAIGELIGAQLRKAGPGTQRERLKRLWAFTVFGLVVNGPVFHFWYGTLEKIA---RKWSKLSGGRQVILFKILLDRLLLTPPYLALTLVGLRWLQDCRLGAAVQASRGIYKQALLTNWK 170          
BLAST of mRNA_L-elsbetiae_contig16939.4651.1 vs. uniprot
Match: A0A836CBT9_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CBT9_9STRA)

HSP 1 Score: 100 bits (249), Expect = 5.270e-24
Identity = 61/132 (46.21%), Postives = 77/132 (58.33%), Query Frame = 1
Query:    4 PLLTKSLTAAVISVLGEIAASAL--------RRLLRRTAAFAFFGLVLNGPVFHWWYGALERAAARRRKPGEPPGGTGDIAFKVALDRFLLTPPYLAITLTSLRLLQGLGAKRSLAETRALYRGALVTNWKV 375
            PLLTKS+TA++++  GE   S L        R   RR  AFA FGLV+NGP FH+WY  LE+ A R  K     GG   I FKV +DR L+TPPYL +TL  LR LQ      +L     +Y+ AL+ NWK+
Sbjct:   37 PLLTKSVTASLMAATGEFVGSYLKKSGPGTPRERARRLGAFAVFGLVVNGPAFHFWYAFLEQLAQRWSKLT---GGHHIILFKVIVDRLLMTPPYLVLTLVGLRWLQDFKLLLALQACALVYKSALLLNWKL 165          
BLAST of mRNA_L-elsbetiae_contig16939.4651.1 vs. uniprot
Match: A0A6G0XN93_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XN93_9STRA)

HSP 1 Score: 86.3 bits (212), Expect = 1.160e-18
Identity = 51/131 (38.93%), Postives = 73/131 (55.73%), Query Frame = 1
Query:    1 RPLLTKSLTAAVISVLGEIAASALRRL------LRRTAAFAFFGLVLNGPVFHWWYGALERAAARRRKPGEPPGGTGDIAFKVALDRFLLTPPYLAITLTSLRLLQGLGAKRSLAETRALYRGALVTNWKV 375
            RPL TK+LT+A + + GEI A  L++       LRR  AF  FG  + GP+ H+WYG LE+   R  K   P         K+ LDR +LTPP++A+T+ +L +L G   + + +  RA Y   L  NW+V
Sbjct:   22 RPLRTKALTSASMGICGEILAHVLKKKPIYTVSLRRVLAFGVFGGAITGPLMHFWYGVLEQVRTRDDKSLTP-------MQKLFLDRLVLTPPFVALTIFALGVLNGASVQAARSNVRATYFSTLFMNWRV 145          
BLAST of mRNA_L-elsbetiae_contig16939.4651.1 vs. uniprot
Match: A0A485LPX3_9STRA (Aste57867_24163 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485LPX3_9STRA)

HSP 1 Score: 83.6 bits (205), Expect = 1.220e-17
Identity = 51/133 (38.35%), Postives = 74/133 (55.64%), Query Frame = 1
Query:    1 RPLLTKSLTAAVISVLGEIAASALRRL------LRRTAAFAFFGLVLNGPVFHWWYGALERAAARRRK--PGEPPGGTGDIAFKVALDRFLLTPPYLAITLTSLRLLQGLGAKRSLAETRALYRGALVTNWKV 375
            +PL TK+ T+A + V GE+ A  L++        RR  AF  +G V+ GP+ H+WYG LER  +   K  P +          K+ +DR LLTPP++A+T+  L +L G  A  + A  RA+Y   L+ NWKV
Sbjct:   22 KPLQTKAATSAAMGVCGEVLAHTLKKKPIYTVSFRRMLAFGLYGGVVTGPIMHYWYGFLERMRSGGNKLTPTQ----------KLLMDRLLLTPPFVALTILMLGVLNGASATAARANLRAVYWTTLLMNWKV 144          
BLAST of mRNA_L-elsbetiae_contig16939.4651.1 vs. uniprot
Match: W7TTQ5_9STRA (Peroxisomal membrane protein 2 n=3 Tax=Monodopsidaceae TaxID=425072 RepID=W7TTQ5_9STRA)

HSP 1 Score: 82.0 bits (201), Expect = 1.600e-16
Identity = 54/129 (41.86%), Postives = 76/129 (58.91%), Query Frame = 1
Query:    1 RPLLTKSLTAAVISVLGEIAASALR----RLLRRTAAFAFFGLVLNGPVFHWWYGALERAAARRRKPGEPPGGTGDIAFKVALDRFLLTPPYLAITLTSLRLLQGLGAKRSLAETRALYRGALVTNWKV 375
            RP+LTK+LT+AVIS LG+I AS+ +    R  RRT  F  FG ++ GP+ H+WYG LE+     +       G  ++A KV LD+ L TPP+LA+TL  LRLL+      +    + +Y   L TN +V
Sbjct:   68 RPVLTKALTSAVISALGDILASSGKGGRGRSGRRTLGFFLFGGLVTGPLCHYWYGLLEKKVRGLQ-------GGKNVAMKVLLDKLLFTPPFLALTLFLLRLLESGRPGAAWRGMKQVYFPTLKTNLQV 189          
BLAST of mRNA_L-elsbetiae_contig16939.4651.1 vs. uniprot
Match: A0A1W0AAY0_9STRA (Uncharacterized protein n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1W0AAY0_9STRA)

HSP 1 Score: 79.7 bits (195), Expect = 2.650e-16
Identity = 52/127 (40.94%), Postives = 67/127 (52.76%), Query Frame = 1
Query:   13 TKSLTAAVISVLGEIAASALRRL------LRRTAAFAFFGLVLNGPVFHWWYGALERAAARRRKPGEPPGGTGDIAFKVALDRFLLTPPYLAITLTSLRLLQGLGAKRSLAETRALYRGALVTNWKV 375
            TK+LT+A I V+GEI A  L+         RR AAF  +G V+  P+ H WYG LE+            G +     K+ LDR +LTPP+L IT+T L LL G   K +    R  Y  AL+ NWKV
Sbjct:   10 TKALTSASIGVVGEIIAHGLKNKSLNGLSTRRMAAFGLYGGVMTAPIMHLWYGFLEKYRVN--------GKSLSPTLKLLLDRLVLTPPFLTITMTCLSLLTGSTIKSTKELLRRNYVAALMMNWKV 128          
BLAST of mRNA_L-elsbetiae_contig16939.4651.1 vs. uniprot
Match: A0A067D856_SAPPC (Uncharacterized protein n=3 Tax=Saprolegnia TaxID=4769 RepID=A0A067D856_SAPPC)

HSP 1 Score: 79.3 bits (194), Expect = 4.670e-16
Identity = 55/131 (41.98%), Postives = 70/131 (53.44%), Query Frame = 1
Query:    1 RPLLTKSLTAAVISVLGEIAASALRRL------LRRTAAFAFFGLVLNGPVFHWWYGALERAAARRRKPGEPPGGTGDIAFKVALDRFLLTPPYLAITLTSLRLLQGLGAKRSLAETRALYRGALVTNWKV 375
            RPL TK++T+AV+   GEI A  L+        L+R   FA +   +  PV H WYG LE+   R    G+     G    K+ LDR LLTPP+LA+TL +L LL G     S A  R+ Y  AL  NWKV
Sbjct:   21 RPLQTKAITSAVMGASGEIVAHGLKYKSLQGLSLQRVLVFALYSGGITAPVMHAWYGFLEQCRVR----GDRLSNNG----KLLLDRLLLTPPFLALTLFALSLLTGASVAASKALVRSKYVTALWMNWKV 143          
BLAST of mRNA_L-elsbetiae_contig16939.4651.1 vs. uniprot
Match: UPI001B88D8BB (peroxisomal membrane protein 2-like n=1 Tax=Gigantopelta aegis TaxID=1735272 RepID=UPI001B88D8BB)

HSP 1 Score: 65.9 bits (159), Expect = 9.750e-11
Identity = 42/132 (31.82%), Postives = 67/132 (50.76%), Query Frame = 1
Query:    1 RPLLTKSLTAAVISVLGEIAASAL--------RRLLRRTAAFAFFGLVLNGPVFHWWYGALERAAARRRKPGEPPGGTGDIAFKVALDRFLLTPPYLAITLTSLRLLQGLGAKRSLAETRALYRGALVTNWK 372
            RP+LTK++T+A IS LG I +  +        +   R   ++A FG  ++GPV H WY  LE     ++        +  +  K+  DR L TPP++   L  + +L+G G K ++   + +Y   L TNWK
Sbjct:   26 RPVLTKAITSACISSLGNILSQLIVPSSQLGNKIAWRSVLSYASFGFFISGPVVHHWYRLLEHLGGSKKD-------STVVIKKILADRLLFTPPFIFTFLYVISILEGHGHKGAMHRIKEVYWYILKTNWK 150          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig16939.4651.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FXU0_ECTSI4.720e-5763.69Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5L3A8_9PHAE3.140e-5664.74Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A836CQ06_9STRA4.620e-2848.09Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A836CBT9_9STRA5.270e-2446.21Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A6G0XN93_9STRA1.160e-1838.93Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
A0A485LPX3_9STRA1.220e-1738.35Aste57867_24163 protein n=1 Tax=Aphanomyces stella... [more]
W7TTQ5_9STRA1.600e-1641.86Peroxisomal membrane protein 2 n=3 Tax=Monodopsida... [more]
A0A1W0AAY0_9STRA2.650e-1640.94Uncharacterized protein n=1 Tax=Thraustotheca clav... [more]
A0A067D856_SAPPC4.670e-1641.98Uncharacterized protein n=3 Tax=Saprolegnia TaxID=... [more]
UPI001B88D8BB9.750e-1131.82peroxisomal membrane protein 2-like n=1 Tax=Gigant... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig16939contigL-elsbetiae_contig16939:2177..3267 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score187.6
Seed ortholog evalue3.6e-45
Seed eggNOG ortholog2880.D7FXU0
Preferred namePXMP2
KEGG koko:K13347
KEGG Pathwayko04146,map04146
Hectar predicted targeting categorysignal peptide
GOsGO:0003674,GO:0005215,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005777,GO:0005778,GO:0005779,GO:0006810,GO:0006811,GO:0006820,GO:0008150,GO:0015267,GO:0015711,GO:0015849,GO:0016020,GO:0016021,GO:0022803,GO:0022857,GO:0031090,GO:0031224,GO:0031231,GO:0031300,GO:0031301,GO:0031903,GO:0032991,GO:0042579,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044425,GO:0044438,GO:0044439,GO:0044444,GO:0044446,GO:0044464,GO:0046942,GO:0051179,GO:0051234,GO:0055085,GO:0071702,GO:0098588,GO:0098805
EggNOG free text desc.Belongs to the peroxisomal membrane protein PXMP2 4 family
EggNOG OGsKOG1944@1,KOG1944@2759
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001
Ec32 ortholog descriptionMpv17/PMP22
Ec32 orthologEc-20_003350.1
Exons3
Model size375
Cds size375
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig16939.4651.1prot_L-elsbetiae_contig16939.4651.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig16939 2177..3267 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815703.6850533-CDS-L-elsbetiae_contig16939:2176..23751622815703.6850533-CDS-L-elsbetiae_contig16939:2176..2375Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16939 2177..2375 -
1691683048.3553948-CDS-L-elsbetiae_contig16939:2176..23751691683048.3553948-CDS-L-elsbetiae_contig16939:2176..2375Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16939 2177..2375 -
1622815703.6962717-CDS-L-elsbetiae_contig16939:2977..30761622815703.6962717-CDS-L-elsbetiae_contig16939:2977..3076Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16939 2978..3076 -
1691683048.3660536-CDS-L-elsbetiae_contig16939:2977..30761691683048.3660536-CDS-L-elsbetiae_contig16939:2977..3076Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16939 2978..3076 -
1622815703.7061064-CDS-L-elsbetiae_contig16939:3190..32671622815703.7061064-CDS-L-elsbetiae_contig16939:3190..3267Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16939 3191..3267 -
1691683048.3751254-CDS-L-elsbetiae_contig16939:3190..32671691683048.3751254-CDS-L-elsbetiae_contig16939:3190..3267Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16939 3191..3267 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig16939.4651.1

>prot_L-elsbetiae_contig16939.4651.1 ID=prot_L-elsbetiae_contig16939.4651.1|Name=mRNA_L-elsbetiae_contig16939.4651.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=125bp
RPLLTKSLTAAVISVLGEIAASALRRLLRRTAAFAFFGLVLNGPVFHWWY
GALERAAARRRKPGEPPGGTGDIAFKVALDRFLLTPPYLAITLTSLRLLQ
GLGAKRSLAETRALYRGALVTNWKV
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mRNA from alignment at L-elsbetiae_contig16939:2177..3267-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig16939.4651.1 ID=mRNA_L-elsbetiae_contig16939.4651.1|Name=mRNA_L-elsbetiae_contig16939.4651.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1091bp|location=Sequence derived from alignment at L-elsbetiae_contig16939:2177..3267- (Laminarionema elsbetiae ELsaHSoW15)
AGGCCACTCCTGACCAAGTCTTTAACGGCGGCCGTGATTTCCGTACTCGG AGAAATCGCGGCCTCCGCGCTACGACGGTCTGCGCCCCCGCCCCCGGCGC CCCCGAACCCGGCCGGGCTCGGACAGGCGCTGGTGGCGGCGAAGGGAGGG CCAGGGGTTGGACCGCGGATTGGGGCACGGGGAGCGCCGAGCCTGCTGAG GAGGACCGCCGCTTTCGCGTTTTTCGGGCTCGTGCTGAACGGGCCTGTGT TCCACTGGTGGTACGGGGCCCTGGAGAGAGCGGCGGCAAGGTGACGATGG GAAGGCTGAGCACGTTCACTGCTGCGCCCCCGCAAAAGGAAGGTTGGAAG AGACGGGACACCGCACCGACCCCCCAATTTATGAGTTTTTGGTGCTGTAG TGCTTTGGGTGTTCGGGGCGCTCTGTGGTCTCCTCACGTCCAGGTGATGC GCTGGCCCTGAATAAGCCTTCGCGGAGCTAGGGTTTGGCGAACAAAATAG CGAAATGCTTCAAGTATAACTGTTACTGAGAAGCATGCTGGCTTGTTCAA TGGTGAGCGGTGCGTGGCTTGTCCGGTTCGCCGTCTGACACCAGGGGGGC TCTGTCAGCAAGAGAGACCAGGGGCACTCCGCGTAGTAGGCTTCCAGGGG CACTCCGCGTATAGGCTTCCTTCGACGCGCGCGGTGGAGCCTGCAAAGCC GGGTCAATACTGCCAACCACCCGCTTGCACGCACGCTGGGCCTCGATGGC CCACCGTCGTGAGTTCCCGTGCCGCGTGGGTAGCACAGGCGTCCCATCGA AAGCCCCGTCTCGTCTGTGTGACGCACAGACATGGACATTCCTTCTGAAG CTCGGCATCCCTGCCCGCACGTTATGTACTTGCACTGAATAGGCGTCGCA AGCCCGGCGAGCCCCCGGGCGGCACCGGCGATATCGCTTTCAAGGTAGCG CTTGATCGCTTCCTCCTGACTCCCCCCTACCTGGCGATCACTCTGACGTC TCTGAGGCTCCTCCAGGGACTCGGCGCGAAGAGGTCGCTCGCGGAGACGA GGGCTCTGTACAGAGGGGCGCTGGTCACGAACTGGAAGGTG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig16939:2177..3267-

>mRNA_L-elsbetiae_contig16939.4651.1 ID=mRNA_L-elsbetiae_contig16939.4651.1|Name=mRNA_L-elsbetiae_contig16939.4651.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=750bp|location=Sequence derived from alignment at L-elsbetiae_contig16939:2177..3267- (Laminarionema elsbetiae ELsaHSoW15)
AGGCCACTCCTGACCAAGTCTTTAACGGCGGCCGTGATTTCCGTACTCGG
AGAAATCGCGGCCTCCGCGCTACGACGAGGCCACTCCTGACCAAGTCTTT
AACGGCGGCCGTGATTTCCGTACTCGGAGAAATCGCGGCCTCCGCGCTAC
GACGCCTGCTGAGGAGGACCGCCGCTTTCGCGTTTTTCGGGCTCGTGCTG
AACGGGCCTGTGTTCCACTGGTGGTACGGGGCCCTGGAGAGAGCGGCGGC
AAGCCTGCTGAGGAGGACCGCCGCTTTCGCGTTTTTCGGGCTCGTGCTGA
ACGGGCCTGTGTTCCACTGGTGGTACGGGGCCCTGGAGAGAGCGGCGGCA
AGGCGTCGCAAGCCCGGCGAGCCCCCGGGCGGCACCGGCGATATCGCTTT
CAAGGTAGCGCTTGATCGCTTCCTCCTGACTCCCCCCTACCTGGCGATCA
CTCTGACGTCTCTGAGGCTCCTCCAGGGACTCGGCGCGAAGAGGTCGCTC
GCGGAGACGAGGGCTCTGTACAGAGGGGCGCTGGTCACGAACTGGAAGGT
GGCGTCGCAAGCCCGGCGAGCCCCCGGGCGGCACCGGCGATATCGCTTTC
AAGGTAGCGCTTGATCGCTTCCTCCTGACTCCCCCCTACCTGGCGATCAC
TCTGACGTCTCTGAGGCTCCTCCAGGGACTCGGCGCGAAGAGGTCGCTCG
CGGAGACGAGGGCTCTGTACAGAGGGGCGCTGGTCACGAACTGGAAGGTG
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