mRNA_L-elsbetiae_contig16400.4354.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig16400.4354.1
Unique NamemRNA_L-elsbetiae_contig16400.4354.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig16400.4354.1 vs. uniprot
Match: A0A2N5K598_9CHLR (Uncharacterized protein n=1 Tax=Chloroflexi bacterium TaxID=2026724 RepID=A0A2N5K598_9CHLR)

HSP 1 Score: 343 bits (880), Expect = 5.790e-103
Identity = 193/494 (39.07%), Postives = 282/494 (57.09%), Query Frame = 1
Query:    1 RGILADRPQGNLHAQAHAKQILHDLDTAVDDWPRFRVDLDVRLVHAAHHMLVAGFDFLDAGEHEELATALLVRGAESLEFAIATTPSHLSSP-DEALKAAAAYHIAGHHARSYVLSEPLVSQEWTDALP---AGLLALLRRDFAGLASQLRGPESLAITSAREVGALLPEGAISEDDALTRIGHDSLLYALRLYVEFVKRGQNALLDEARALCRTVAELGRESRHIDLWWWGRATEHLLRELGDSSLWRCIGDLGPDTPYGRPINRYIEGALGSTPCTVSLWPSQRKAIEIIAADGAPSFCVRMPTSAGKTKIAELAIVRAILDAGLGESAKCLYIAPFRSLAVEIESTLSRGLKPLGIRVSEVYGGFDLTAHDERLIAETQALIATPEKLDAALRLAPELFDDVRLVAIDEGHIAGDLSERGIRAEFLFTRLLRKLGRERCRHLFVSAVLPNPAEFATWIAGDDENLVDSDWRPARLVLGECQWNGSRLRIEF 1470
            + +L D    N  AQAH++ +L  L    + WPR+  +L  +L H AH++L  G    D          LL  GAE LE+     P+       + L AA  Y+IAG++AR++VL + L   E +DALP   A L  LL +D AGL   +         S   + A L +G ++E+  L+ + H SL YA  +++E+ + G+  LL++A  +        +++   D WW    T ++ RE     LW  +  L  D P G  +  YI G        + LW SQ  A+  +      S+C+ MPTSAGKT++AE+AI+R +LD      AKCLYIAP+RSLAVE+E +L +   PLG+RVSE+YGGF+L+A +  L+  T+ +IATPEK+DA LR + EL   +RLV +DEGH+   LS+RG+R E    RL+R+   +  R+L +SAVLPNP +FA WIA   EN++ + WRP+R ++GE  W+G R RIE+
Sbjct:   14 QALLDDETFRNRVAQAHSRAVLQRLAVPAEQWPRYDTELTDKLHHTAHYLLWHGLQLKDDATTAASGDRLLKDGAEILEYLYGQAPAPRGDALHQCLNAALGYYIAGYYARAFVLMQQL---EISDALPQELAVLRQLLIKDLAGLRQTVDTFLLDPAHSDEAMVAALRDGVMTEEGVLSHVLHTSLNYAFSVFLEYPRTGRRVLLEQALNILDHGIATAQKTHFADWWWLLTCTRYIFREYDTHCLWTQLNPLNDDDPQGSIVAPYIRGCYRRRVPIIELWRSQTMAVPFVNDPDRQSYCLSMPTSAGKTRVAEVAILRFLLDTQHNPDAKCLYIAPYRSLAVELEQSLQQSFLPLGVRVSELYGGFELSATERLLLERTRVMIATPEKIDALLRYSEELAAQIRLVILDEGHMVS-LSDRGLRYELFVHRLVRRFADQGVRYLLLSAVLPNPGDFAQWIAERAENVIQTTWRPSRQLVGELIWDGERARIEY 503          
BLAST of mRNA_L-elsbetiae_contig16400.4354.1 vs. uniprot
Match: UPI00064FF106 (DEAD/DEAH box helicase n=1 Tax=Methanobacterium TaxID=2160 RepID=UPI00064FF106)

HSP 1 Score: 331 bits (848), Expect = 1.460e-98
Identity = 187/491 (38.09%), Postives = 287/491 (58.45%), Query Frame = 1
Query:   34 LHAQAHAKQILHDLDTAVDDWPRFRVDLDVRLVHAAHHMLVAGFDFLDAGEHEELATALLVRGAESLEFAIATTPSHLSSPDEAL-KAAAAYHIAGHHARSYVLSEPLVSQ-EWTDALPAGLLA-LLRRDFAGLASQLRGPESLAITSARE-----VGALLPEGAISEDDALTRIGHDSLLYALRLYVEFVKRGQNALLDEARALCRTVAELGRESRHIDLWWWGRATEHLLRELGDSSLWRCIGDLGPDTPYGRPINRYIEGALGSTPCTVSLWPSQRKAIEIIAADGAPSFCVRMPTSAGKTKIAELAIVRAILDAGLGESAKCLYIAPFRSLAVEIESTLSRGLKPLGIRVSEVYGGFDLTAHDERLIAETQALIATPEKLDAALRLAPELFDDVRLVAIDEGHIAGDLSERGIRAEFLFTRLLRKLGRERCRHLFVSAVLPNPAEFATWIAGDDENLVDSDWRPARLVLGECQWNGSRLRIEFDHDV 1482
            L AQAHA+ I+ +L+     WP F+ DLD+RL + A+  +++  + L+  ++++ A   L +GAE+LE+         S  DE +  +  AY+I+G++A +YVLS+ +  + E +D  P  L+A  L++D  G  ++     +L I + R+     +   + EG I E++A++R+ + S+  A+ L+V +VK G+      +  +      L ++ R +D WWW  +   + +E  ++SLW+ +     D        +YI   L   P  + LWPSQ  A+ ++  +   SFC++MPTSAGKT+IAEL I+R  LD+ L E  KC+YIAPFRSLA+EIE         LG +VSE+YGGFDL   +  LI ET  L+ TPEK DA LR  P++ +D+ L+ IDEGHI  D ++RG+  EF   RLL +   + CR LF+SAVLPN  +FA WI G    L++SDWRP+RL+LG   W G    I + H++
Sbjct:   25 LEAQAHARSIISNLNPNKAQWPHFKKDLDIRLYYMANFQILSALNLLEFEKYQKPAKDYLKKGAEALEYLYKYPGCDSSISDEVIFNSILAYYISGNYASAYVLSKEIDGKLELSD--PQKLIATFLKKDLKGALTK-----ALNILNDRKYKDQIIANEILEGNIDENEAISRVFNYSIAKAVSLHVGYVKTGKLEYYQNSIKIIEHSINLAKKHRFVDWWWWLYSLSFIFKEYYENSLWKQLDSFKEDNKD--ITKKYIRSQLSHKPPVIELWPSQVSAMPMVIDEQKRSFCLKMPTSAGKTRIAELTILRFFLDS-LEEPRKCVYIAPFRSLALEIEKKFRENFNLLGYKVSEIYGGFDLNPAESTLIQETHILVLTPEKFDAILRYIPDIAEDIGLIIIDEGHII-DTTKRGLMFEFFIQRLLNRFNDKDCRFLFISAVLPNAKQFAQWITGSSNQLIESDWRPSRLMLGRLTWRGESAWINYTHNL 504          
BLAST of mRNA_L-elsbetiae_contig16400.4354.1 vs. uniprot
Match: UPI001956AF74 (DEAD/DEAH box helicase n=1 Tax=Desulforadius tongensis TaxID=1216062 RepID=UPI001956AF74)

HSP 1 Score: 280 bits (717), Expect = 3.540e-80
Identity = 180/494 (36.44%), Postives = 258/494 (52.23%), Query Frame = 1
Query:    7 ILADRPQGNLHAQAHAKQILHDLDTAVDDWPRFRVDLDVRLVHAAHHMLVAGFDFLDAGEHEELATALLVRGAESLEFAIATTPSHLSSPDEALK-AAAAYHIAGHHARSYVLSEPLVSQEWTDALPAGLLALLRRDFAGLASQLRGPESLAITSAREVGALLPEGAISED----DALTRIGHDSLLYALRLYVEFVKRGQNALLDEARALCRTVAELGRESRHIDLWWWGRATEHLLRELGDSSLWRCIGDLGPDTPYGRPINRYIEGALGSTPCTVSLWPSQRKAIEIIAADGAPSFCVRMPTSAGKTKIAELAIVRAILDAGLGESAK-CLYIAPFRSLAVEIESTLSRGLKPLGIRVSEVYGGFDLTAHDERLIAETQALIATPEKLDAALRLAPELFDDVRLVAIDEGHIAGDLSERGIRAEFLFTRLLRKLGRERCRHLFVSAVLPNPAEFATWIAGDDENLVDSDWRPARLVLGECQWNGSRLRIEF 1470
             L  R   N   QA+++ +L +L    ++WP +   +   LV  AH++L+         EH ELA   L   AE LEF  +    + +     L  +A AY+IAG +ARSYVL + L     +      L  L  +D       LR    L +         L E   SED    + +  +  ++   AL  YVE+VK G+ AL D+A        +L RE + +D WW    T ++L E  D SLW+ + D       G  + +YI     S P  + LWPSQ+ A+  I       + +RMPTSAGKT+IAEL I++ +LD    E  K C+YIAPFRSLA E+E  L +   PLG  V+E+YGGF+    +  ++ E++ ++ATPEK+DA LR   +L D + LV +DEGHI    S RG++ E    RL R L     R+LF+SAVLPN  EF+ W+ G  E ++ S+WRPAR +  E +W  S  R+E+
Sbjct:   17 FLQSRSFKNSKNQAYSRSVLINLHAPEEEWPDYNFSVS-HLVEGAHYLLMNAVLMKPFAEHAELAREALKEAAEVLEFLYSNKTENKAEQIRQLTFSALAYYIAGFYARSYVLMKELEIPTDSPVEYRALRLLFLKDI----KVLRSLVYLELKRINLSDEGLKEKWNSEDADPDEIIWELVQETYFRALSYYVEYVKCGEKALFDKAINFLEIGIDLAREHQFVDWWWVFYFTRYILLEYWDHSLWKNL-DFLLVHENGDLVRKYIIHQYKSMPSVIELWPSQKTAVPHIFEPERGDYVLRMPTSAGKTRIAELTILQFLLD----EPTKLCVYIAPFRSLAAEVEKGLKKIFHPLGFGVTELYGGFEFGQFERLIVEESRIVVATPEKIDAFLRYNTDLIDQIGLVIVDEGHIVS-FSHRGLKYEMFLQRLKRLLQYNLPRYLFISAVLPNTEEFSEWLCGSSEQIIASNWRPARFLTAELEWRSSGGRLEY 499          
BLAST of mRNA_L-elsbetiae_contig16400.4354.1 vs. uniprot
Match: A0A2H0P5I4_9BACT (DEAD/DEAH box helicase (Fragment) n=1 Tax=Elusimicrobia bacterium CG11_big_fil_rev_8_21_14_0_20_64_6 TaxID=2014311 RepID=A0A2H0P5I4_9BACT)

HSP 1 Score: 231 bits (590), Expect = 1.110e-66
Identity = 164/483 (33.95%), Postives = 246/483 (50.93%), Query Frame = 1
Query:   31 NLHAQAHAKQILHDLDTAVDDWPRFRVDLDVRLVHAAHHMLVAGFDFLDAGEHEELATALLVRGAESLEFAIATT-PSHLSSPDEALKAAAAYHIAGHHARSYVLSEPLVSQEWTDALPAGLLA-LLRRDFAGLASQLRGPESLAITSAREVGALLPEGAISEDDALTRIGHDSLLYALRLYVEFVKRGQNALLDEARALCRTVAELGRESRHIDLWWWGRATEHLLRELGDSSLWRCIGD-LGPDTPYGRPINRYIEGALGSTPCTVSLWPSQRKAIEIIAADGAPSFCVRMPTSAGKTKIAELAIVRAILDAGLGESAKCLYIAPFRSLAVEIESTLSRGLKPLGIRVSEVYGGFDLTAHDERLIAETQALIATPEKLDAALRLAPELFDDVRLVAIDEGHIAGDLSERGIRAEFLFTRLLRKLGRERCRHLFVSAVLPNPAEFATWIAGDDENLVDSDWRPARLVLGECQWNGSRLRIEF 1470
            NL AQ+ AKQ+L ++    +++PRF   LD R+  AA+ ML AG   ++ GE  E     L   A+ LE A  T   ++ +S    L  A A++  G ++R++VL    ++   T +  AG++A  LR+D A L S+L    ++ +    E      E +  +D AL       +  ++ +  E+   GQ  LL +A A+      +   S +   WW  R    +L++ G  +LW  +    GPD      I  Y+       P  + LW SQ  A+ +          + + TSAGKT++AELAI++ +        +K LY+APFRSLA E+E T S+ L PLG  VS +YGG   +  D  L+ E    IATPEK  A LR APEL + V+LV +DEGH+ GD + R +R E     L  +  +   R L +SAVLPN  + A W+ GD + LV S+W+P+    G  +W  S   IE+
Sbjct:   22 NLVAQSKAKQVLREVRENPENFPRFETTLDDRVTFAAYTMLAAGCSLIERGEVPE-GRGELQAAADILESAHRTEITNNQTSALHCLIGAMAFYSCGQYSRAFVL----INNVETASSSAGVIASFLRKDRARLISKL---SAILLAEPPE----FTESSEFDDWALAVC----VARSVSIVTEYSFSGQAGLLAQADAILDDAMAISEGSSNPSYWWLARLLRLMLKDYGSGALWAVLPPYFGPDGTT--EIESYVRLLAFQKPPVLELWQSQISALHLALDSENQGGVINLRTSAGKTRVAELAILQTLRA---DPESKILYLAPFRSLAFELERTFSKTLLPLGYSVSHLYGGSRFSGVDRELMNEGHITIATPEKAKAMLRAAPELLESVKLVIVDEGHLLGD-NVRNVRNELFLEHLRLRAHQRGARVLLLSAVLPNAEDLAAWVGGDAKALVKSNWKPSAERFGNLRWKSSGASIEW 482          
BLAST of mRNA_L-elsbetiae_contig16400.4354.1 vs. uniprot
Match: A0A7U7G873_9GAMM (Helicase-like:Type III restriction enzyme, res subunit:DEAD/DEAH box helicase-like n=1 Tax=Candidatus Contendobacter odensis Run_B_J11 TaxID=1400861 RepID=A0A7U7G873_9GAMM)

HSP 1 Score: 231 bits (589), Expect = 9.600e-63
Identity = 171/488 (35.04%), Postives = 254/488 (52.05%), Query Frame = 1
Query:   31 NLHAQAHAKQILHDLDTAVDDWPRFRVDLDVRLVHAAHHMLVAGFDFLDAGE----HEELATALLVRGAESLEFAIATTPSHL-SSPDEALKAAAAYHIAGHHARSYVLSEPLVSQEWTDALPAGLLA-LLRRDFAGLASQLRGPESLAITSAREVGALLPEGAISEDDALTRIGHDSLLYALRLYVEFVKRGQNALLDEARALCRTVAELGRESRHIDLWWWGRATEHLLRELGDSSLWRCIGDL-GPDTPYGRPINRYIEGALGSTPCTVSLWPSQRKAIEIIAADGAPSFCVRMPTSAGKTKIAELAIVRAILDAGLGESAKCLYIAPFRSLAVEIESTLSRGLKPLGIRVSEVYGGFDLTAHDERLIAETQALIATPEKLDAALRLAPELFDDVRLVAIDEGHIAGDLSERGIRAEFLFTRLLRKLGRER-CRHLFVSAVLPNPAEFATWIAGDDENLVDSDWRPARLVLGECQWNGSRLRIEF 1470
            NL AQ+ AKQ+L ++     ++P F  +LD R+   A+ +L AG   ++ G+    H EL TA     A+ LE A  T  ++  +S    L  A A++  G ++R++VL   +  +E T A  AG++A  LR+    L S+L      A+  A          +  +D ALT      +  ++ L  E    G+  LL  A  + R    +   + H   WW  R    + ++ G+SSLW  +    GPD      ++ Y++      P  + LW SQ  A+++          + + TSAGKT++AELAI+  +       SAK LY+APFRSLA E+E T +R L PLG  VS +YGG   +  D  ++ E +  IATPEK  A LR APELF+ V+L+ IDEGH+ G  +ER +R E +F   LR L R+R  R L +SAVLPN  + A W+ G  + LV S+W+P+    G  QW  S + IE+
Sbjct:   22 NLIAQSKAKQVLREVREIPANFPNFAPELDDRVTFVAYGLLAAGCSLIEQGQTSEGHAELETA-----ADILESAHQTEVANCETSALHCLIGAMAFYACGQYSRAFVLIGSV--EELTPA--AGIVASFLRKSRPRLISKLN-----AVLLAPSPS--FDNSSQFDDWALTIC----VARSVALVAEHAISGEPELLANADVVLRDAMTISEGASHPTFWWLARLLRLMFKDYGNSSLWSVLPPFFGPDGIVH--VDSYVKLLAFLKPPVIELWQSQTTALQLALNPKNKGGVINLRTSAGKTRVAELAILHTL---STDPSAKVLYLAPFRSLAFELEKTFARTLVPLGYSVSHLYGGSRFSGVDREMVNEGRITIATPEKAKAMLRAAPELFEAVKLIVIDEGHLLGG-NERNVRNE-MFLEHLRLLARQRGARILLLSAVLPNAEDLAAWVGGGVDALVKSNWKPSAERFGTLQWKASGVSIEW 482          
BLAST of mRNA_L-elsbetiae_contig16400.4354.1 vs. uniprot
Match: Q0YV15_9CHLB (Helicase-like:Type III restriction enzyme, res subunit:DEAD/DEAH box helicase-like n=2 Tax=Chlorobium TaxID=1091 RepID=Q0YV15_9CHLB)

HSP 1 Score: 228 bits (581), Expect = 1.220e-61
Identity = 176/496 (35.48%), Postives = 242/496 (48.79%), Query Frame = 1
Query:   10 LADRPQGNLHAQAHAKQILHDLDTAVDDWPRFRVDLDVRLVHAAHHMLVAGFDFLDAGEHEELATALLV--RGAESLEFAIATTPSHLS-SPDEALKAAAAYHIAGHHARSYVLSEPLVSQEWTDALPAGLLA-LLRRDFAGLASQLRGPESLAITSAREVGALLPEGAISEDDALT--RIGHDSLLYALRLYV-EFVKRGQNALLDEARALCRTVAELGRESRHIDLWWWGRATEHLLRELGDSSLWRCIGDL-GPDTPYGRPINRYIEGALGSTPCTVSLWPSQRKAIEIIAADGAPSFCVRMPTSAGKTKIAELAIVRAILDAGLGESAKCLYIAPFRSLAVEIESTLSRGLKPLGIRVSEVYGGFDLTAHDERLIAETQALIATPEKLDAALRLAPELFDDVRLVAIDEGHIAGDLSERGIRAEFLFTRLLRKLGRERC-RHLFVSAVLPNPAEFATWIAGDDENLVDSDWRPARLVLGECQWNGSRLRIEF 1470
            L   P  N  +QA AKQ+L ++    + +P F   LD R+   A+ ML AG   +   E + L   +L      E LE    T+    S S    L  A A++  GH++R+YVL    +    T    AG++A  LR+DFA L  +L                LL E  + E+D +   R   D L+     +V E    G+  LL  A A+      +     +   WW  R    +  + G  SLW C+    GPD      I  Y        P    LW SQ  A+E+          V + TSAGKT++AELAI++   +     +AK LY+APFRSLA E+E T  + L  LG  +S +YGG   +  D  ++ E   LIATPEK  A LR APELFD+V+L+ IDEGH+ G  SER ++ E LF   LR L R R  R L +SAVLPN  E ATWI G +  L  SDW+P+    G  +W  + ++IE+
Sbjct:   24 LRVEPIPNFISQAKAKQLLQEVHELPEHFPGFTEGLDDRVTFVAYRMLSAGCSLV---EQDLLGEGILQLHSAGELLESTHRTSARTESVSGFHCLIGAMAFYACGHYSRAYVL----IKATETITPVAGIIASFLRKDFAKLIPRLND-------------VLLREQPLYEEDFIEADRRALDLLIARSVAHVLEHSISGEAELLTNADAILADAMIISEAGANPSFWWIARILRLMFNDYGRGSLWVCLPPFFGPDGKS--TIGDYASLLALVKPPVTELWRSQLAALELALNGDNLGGVVNLRTSAGKTRVAELAILK---ELRANSTAKVLYLAPFRSLAFELERTFEKYLGALGFSISHLYGGSRFSGVDREMVLEAHLLIATPEKTKAMLRAAPELFDNVKLIVIDEGHLLGG-SERNVKNE-LFLEHLRLLSRNRSTRILLLSAVLPNATELATWIGGSESALAQSDWKPSDERFGVLRWTKNGVQIEW 492          
BLAST of mRNA_L-elsbetiae_contig16400.4354.1 vs. uniprot
Match: D8K2R9_DEHLB (DEAD/DEAH box helicase domain protein n=1 Tax=Dehalogenimonas lykanthroporepellens (strain ATCC BAA-1523 / JCM 15061 / BL-DC-9) TaxID=552811 RepID=D8K2R9_DEHLB)

HSP 1 Score: 223 bits (569), Expect = 9.400e-61
Identity = 164/502 (32.67%), Postives = 245/502 (48.80%), Query Frame = 1
Query:    1 RGILADRPQG---NLHAQAHAKQILHDLDTAVDDWPRFRVDLDVRLVHAAHHMLVAGFDFLDAGEHEELATALLVRGAESLEFAIATTPSHLSSPDEALKAAAAYHIAGHHARSYVLSEPLVSQEWTDALPAGLLALLRRDFAGLASQLRGPESLAITSAREVGALLPEGAISEDDALTRIGHDSLLYALR---LYVEFVKRGQNALLDEARALCRTVAELGRESRHIDLWWWGRATEHLLRELGDSSLWRCIGDLGPDTPYGRPINRYIEGALGSTPCTVSLWPSQRKAIEIIAADGAPSFCVRMPTSAGKTKIAELAIVRAILDAGLGESAKCLYIAPFRSLAVEIESTLSRGLKPLGIRVSEVYGGFDLTAHDERLIAETQALIATPEKLDAALRLAPELFDDVRLVAIDEGHIAGDLSERGIRAEFLFTRLLRKLGRER-CRHLFVSAVLPNPAEFATWIAGDDENLVDSDWRPARLVLGECQWNGSRLRIEFDHDVS 1485
            R IL     G   NL AQ+ A+ +L+++  + D++P F   L  ++  +A+ +L AG    +A   +E  TA+    A  +        + L+S    L A+ A+H +G ++R++V               A LL  L R  A L    + P  + +    E    L     +  DA +   H   L   R   L +E+   G   LL  +        EL  +      WW  R    ++   G +SLW  +    P       ++RYI     ++     LW SQ  AI I    G     V M TS+GKT++AELAI++ + +      AK LY+APFRSLA+EIE TL +     G  VS +YGGF L++ D ++  ++   IATPEK+ A LR +PEL +D+RL+ IDEGH+ G   +R ++ E +F   LR L R   CR L +SAVLPNP E A W+ GD  N+  S W+P+    G  +W GS +RI++  D +
Sbjct:    9 REILQQHQAGHIENLFAQSSARYVLYEVGESEDNFPSFDAALTDKVTLSAYSILAAGVSLAEARLTDEAITAMEEAAALLINVHAPYAETELTSSFHVLIASMAFHASGQYSRAFVYIRK-----------AELLTPLARIVASLIR--KRPADVIL----EANQYLLADLSTMTDAWSLCDHAVTLSISRSTSLVLEYFATGNQQLLAASIENLNIAMELASDYDSPSHWWIARLLRLMISGNGQASLWHALPPYFPGD--SSLLSRYIRLHAFASKSVTELWRSQLDAIPITLGQGRTGAVVNMRTSSGKTRVAELAILQTLHE---NPDAKILYLAPFRSLALEIEQTLGQIFDRCGFLVSHLYGGFRLSSADRQIAKDSTITIATPEKIRAILRSSPELLNDIRLIIIDEGHLIG-ADKRYVKNE-IFIDHLRVLARSSACRILLLSAVLPNPDELAVWLTGDVGNVARSGWKPSSERFGILRWQGSSVRIDWKGDFA 486          
BLAST of mRNA_L-elsbetiae_contig16400.4354.1 vs. uniprot
Match: A0A661R4M0_9DELT (DEAD/DEAH box helicase (Fragment) n=1 Tax=Deltaproteobacteria bacterium TaxID=2026735 RepID=A0A661R4M0_9DELT)

HSP 1 Score: 214 bits (545), Expect = 1.650e-60
Identity = 147/401 (36.66%), Postives = 217/401 (54.11%), Query Frame = 1
Query:  286 LKAAAAYHIAGHHARSYVLSEPLVSQEWTDALPAGLLALLRRDFAGLASQLRGPESLAITSAREVGALLPEGAISEDDALTRIGHDSLLYALRLYVEFVKRGQNALLDEARALCRTVAELGRESRHIDLWWWGRATEHLLRELGDSSLWRCIGDL-GPDTPYGRPINRYIEGALGSTPCTVSLWPSQRKAIEIIAADGAPSFCVRMPTSAGKTKIAELAIVRAILDAGLGESAKCLYIAPFRSLAVEIESTLSRGLKPLGIRVSEVYGGFDLTAHDERLIAETQALIATPEKLDAALRLAPELFDDVRLVAIDEGHIAGDLSERGIRAEFLFTRLLRKLGR-ERCRHLFVSAVLPNPAEFATWIAGDDENLVDSDWRPARLVLGECQWNGSRLRIEFDHDV 1482
            L ++ A++ AGH++R++V  + + SQ    A    + A +R+D   L  +L     L  T A E    L E  ++   A+ R        +L + +E++  G    +D A       A +         WW  R  + +L  LGD+SLW+ +     P+T     + RYI     S P  V LW SQR A+ +          V + TSAGKT+IAELAI++ +       SA+ +Y+APFRSLA+E+E TL+     LG +VS +YGG  +++ D  L AE+  +IATPEK  A  R AP+LF++V+L+ +DEGH+ G  SER ++ E LF   LR L R    R L +SAVLPNP E A W+ GD + +  S W+P+    G  +WNGSR+RI++  DV
Sbjct:    2 LVSSVAFYAAGHYSRAFVTIQNVESQT---AAVGIIAAFIRKDIKTLIQRLN-EVLLRNTPAFEDQTDLDEWVVTV--AIAR--------SLAMALEYLYTGTRDFIDAADRQLEDAAIVASTGHFPAYWWTVRLLKLMLGNLGDASLWQILPPFFDPETKS--TLARYIRLMAYSRPPVVELWASQRAALPMALNTTNRGGVVNLRTSAGKTRIAELAILQTLAS---DSSAQIIYLAPFRSLAMEVEQTLAASFSWLGFKVSHLYGGSRVSSVDTELAAESSIMIATPEKTRALFRAAPDLFENVKLIIVDEGHLIGP-SERFVKNE-LFVDHLRSLARTSSARILLLSAVLPNPQELAEWVTGDSDAVATSRWKPSVERYGLLRWNGSRVRIDWLGDV 381          
BLAST of mRNA_L-elsbetiae_contig16400.4354.1 vs. uniprot
Match: A0A7Y4X8Y7_9BACT (DEAD/DEAH box helicase (Fragment) n=1 Tax=Acidobacteria bacterium TaxID=1978231 RepID=A0A7Y4X8Y7_9BACT)

HSP 1 Score: 218 bits (555), Expect = 1.300e-58
Identity = 162/494 (32.79%), Postives = 245/494 (49.60%), Query Frame = 1
Query:    4 GILADRPQGNLHAQAHAKQILHDLDTAVDDWPRFRVDLDVRLVHAAHHMLVAGFDFLDAGEHEELATALLVRGAESLEFAIATTPSHLSSPDE----ALKAAAAYHIAGHHARSYVLSEPLVSQEWTDALPAGLLALLRRDFAGLASQLRGPESLAITSAREVGALLPEGAISEDDALTRIGHDSLLYALRLYVEFVKRGQNALLDEARALCRTVAELGRESRHIDLWWWGRATEHLLRELGDSSLWRCIGDLGPDTPYGRPINRYIEGALGSTPCTVSLWPSQRKAIEIIAADGAPSFCVRMPTSAGKTKIAELAIVRAILDAGLGESAKCLYIAPFRSLAVEIESTLSRGLKPLGIRVSEVYGGFDLTAHDERLIAETQALIATPEKLDAALRLAPELFDDVRLVAIDEGHIAGDLSERGIRAEFLFTRLLRKLGRER-CRHLFVSAVLPNPAEFATWIAGDDENLVDSDWRPARLVLGECQWNGSRLRIEF 1470
            G L  +   NL AQA A  +LH++  A +++P+F  +L  R+  AA+ +L +G   ++ GE  E    +L   A  L++       H++   E       AA A++ +G ++R++V +  +   E T A+   + A LR++   L  QL           RE      E  + E      I   S+ YA     EF++ GQ ALL+ A         +     +   WW  R    +LR+LG++S W+ +     +  +   ++ YI            +W SQR A+ +          + + TSAGKT+IAE AI++ +L       A+  Y+APFRSLA E+E +L      +G RVS +YGGF  +  D  L  E    IATPEK  A  R APELF +V+L  +DEGH+ GD SER +R E +F   LR   RE+  R L +SAVLPN  E A WI G    +  S+W+P+    G  +W GSR++I++
Sbjct:   13 GQLQVQEVENLFAQAFASHVLHEVKEAPENFPQFDPNLHDRVTFAAYLLLASGCSVIEQGERFE-GNGILEHAAAQLQY---VHGPHIADSHERSFHVFVAAMAFYASGQYSRAFVTARSVA--EPTAAMKI-ICAYLRKETFALDEQLDN------VLLRETPDFQEESELDEWAITVAIAR-SIAYAS----EFIRTGQPALLNNADDQLSDAIIIASAGNYPAWWWVVRLLRLMLRDLGNASPWQVLPPYFDNETHAA-LSGYIRHLAFLDRPVSEIWTSQRAALPLALNSLNRGAVINLRTSAGKTRIAEFAILQTLLT---NPDARIFYLAPFRSLAFEVEHSLGATFTKIGYRVSHLYGGFRASTVDIELADEAAITIATPEKTRALFRSAPELFQNVKLFIVDEGHLIGD-SERDVRNE-IFLDHLRIFAREKGARILLLSAVLPNAQEVAEWITGTQSAVARSEWKPSTERQGLLRWTGSRVKIDW 482          
BLAST of mRNA_L-elsbetiae_contig16400.4354.1 vs. uniprot
Match: A0A497IMW9_9EURY (Helicase ATP-binding domain-containing protein (Fragment) n=1 Tax=Methanosarcinales archaeon TaxID=2250255 RepID=A0A497IMW9_9EURY)

HSP 1 Score: 206 bits (525), Expect = 4.720e-58
Identity = 137/392 (34.95%), Postives = 208/392 (53.06%), Query Frame = 1
Query:    7 ILADRPQGNLHAQAHAKQILHDLDTAVDDWPRFRVDLDVRLVHAAHHMLVAGFDFLDAGEHEELATALLVRGAESLEFAIATT-PSHLSSPDEALKAAAAYHIAGHHARSYVLSEPLVSQEWTDALPAGLLALLRRDFAGLASQLRGPESLAITSAREVGALLPEGA----ISEDDALTRIGHDSLLYALRLYVEFVKRGQNALLDEARALCRTVAELGRESRHIDLWWWGRATEHLLRELGDSSLWRCIGDLGPDTPYGRPINRYIEGALGSTPCTVSLWPSQRKAIEIIAADGAPSFCVRMPTSAGKTKIAELAIVRAILDAGLGESAKCLYIAPFRSLAVEIESTLSRGLKPLGIRVSEVYGGFDLTAHDERLIAETQALIATPEKLDA 1167
            +  +R   N  +QA+++ +L  L     DWP +   LD  L + A  +   G    ++  ++  A   +  GA+ LEF  AT    ++   ++   +A  Y+I+G++AR++VL + L S+         L  L  +D  G+ S +    S  + S     + L  G     ++E D   RI   +L  AL  ++EF K G   LL +A        EL  + R++D WW   A ++L  E  ++SLWR +  L  D   G  I  YI   L      + LW SQ +A+  I      S+ ++MPTS+GKTKIAELAI+RA+LD    + +KC+Y+AP+R+LAVEIE  L    + LGI VSE+YGGFDL+A +  LI +++ LIATPEK+DA
Sbjct:   15 LANNRKFQNFVSQAYSRSVLRQLRANPRDWPSYANTLDSDLHYTAFFLSWQGLILKESEAYKVEADNYIKEGAQILEFLYATALKENVERIEQIFNSALGYYISGYYARAFVLMKDLESENSLPQEFEVLKKLFLKDIRGMRSLI----SSVLFSQEYFDSTLASGLRDKILTEPDVFERIFRATLNKALSYFIEFPKNGYEKLLRQAIFFIEHGIELASKLRYVDWWWLFLALKYLFNEYKENSLWRNLAPLISDDASGELIVPYIRNNLKKRIPLLELWRSQIEALPKINDPDRKSYILKMPTSSGKTKIAELAILRALLD---DKESKCIYLAPYRALAVEIERELRESFRGLGIMVSEIYGGFDLSAVERHLIEKSKVLIATPEKMDA 399          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig16400.4354.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2N5K598_9CHLR5.790e-10339.07Uncharacterized protein n=1 Tax=Chloroflexi bacter... [more]
UPI00064FF1061.460e-9838.09DEAD/DEAH box helicase n=1 Tax=Methanobacterium Ta... [more]
UPI001956AF743.540e-8036.44DEAD/DEAH box helicase n=1 Tax=Desulforadius tonge... [more]
A0A2H0P5I4_9BACT1.110e-6633.95DEAD/DEAH box helicase (Fragment) n=1 Tax=Elusimic... [more]
A0A7U7G873_9GAMM9.600e-6335.04Helicase-like:Type III restriction enzyme, res sub... [more]
Q0YV15_9CHLB1.220e-6135.48Helicase-like:Type III restriction enzyme, res sub... [more]
D8K2R9_DEHLB9.400e-6132.67DEAD/DEAH box helicase domain protein n=1 Tax=Deha... [more]
A0A661R4M0_9DELT1.650e-6036.66DEAD/DEAH box helicase (Fragment) n=1 Tax=Deltapro... [more]
A0A7Y4X8Y7_9BACT1.300e-5832.79DEAD/DEAH box helicase (Fragment) n=1 Tax=Acidobac... [more]
A0A497IMW9_9EURY4.720e-5834.95Helicase ATP-binding domain-containing protein (Fr... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig16400contigL-elsbetiae_contig16400:2286..3770 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeArchaea
Seed ortholog score332.8
Seed ortholog evalue2.7e-88
Seed eggNOG ortholog386456.JQKN01000015_gene2904
Hectar predicted targeting categoryother localisation
EggNOG free text desc.DEAD/DEAH box helicase
EggNOG OGs2Y43S@28890,COG1204@1,arCOG00553@2157
COG Functional cat.K
Best tax levelEuryarchaeota
Best eggNOG OGNA|NA|NA
Exons1
Model size1485
Cds size1485
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815678.0579011-CDS-L-elsbetiae_contig16400:2285..37701622815678.0579011-CDS-L-elsbetiae_contig16400:2285..3770Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16400 2286..3770 +
1691683032.3176692-CDS-L-elsbetiae_contig16400:2285..37701691683032.3176692-CDS-L-elsbetiae_contig16400:2285..3770Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16400 2286..3770 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig16400.4354.1prot_L-elsbetiae_contig16400.4354.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig16400 2286..3770 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig16400.4354.1

>prot_L-elsbetiae_contig16400.4354.1 ID=prot_L-elsbetiae_contig16400.4354.1|Name=mRNA_L-elsbetiae_contig16400.4354.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=495bp
RGILADRPQGNLHAQAHAKQILHDLDTAVDDWPRFRVDLDVRLVHAAHHM
LVAGFDFLDAGEHEELATALLVRGAESLEFAIATTPSHLSSPDEALKAAA
AYHIAGHHARSYVLSEPLVSQEWTDALPAGLLALLRRDFAGLASQLRGPE
SLAITSAREVGALLPEGAISEDDALTRIGHDSLLYALRLYVEFVKRGQNA
LLDEARALCRTVAELGRESRHIDLWWWGRATEHLLRELGDSSLWRCIGDL
GPDTPYGRPINRYIEGALGSTPCTVSLWPSQRKAIEIIAADGAPSFCVRM
PTSAGKTKIAELAIVRAILDAGLGESAKCLYIAPFRSLAVEIESTLSRGL
KPLGIRVSEVYGGFDLTAHDERLIAETQALIATPEKLDAALRLAPELFDD
VRLVAIDEGHIAGDLSERGIRAEFLFTRLLRKLGRERCRHLFVSAVLPNP
AEFATWIAGDDENLVDSDWRPARLVLGECQWNGSRLRIEFDHDVS
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mRNA from alignment at L-elsbetiae_contig16400:2286..3770+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig16400.4354.1 ID=mRNA_L-elsbetiae_contig16400.4354.1|Name=mRNA_L-elsbetiae_contig16400.4354.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1485bp|location=Sequence derived from alignment at L-elsbetiae_contig16400:2286..3770+ (Laminarionema elsbetiae ELsaHSoW15)
CGCGGCATCCTCGCAGACAGACCTCAAGGCAACCTCCATGCACAGGCTCA CGCGAAACAGATACTCCATGATCTCGACACAGCTGTCGATGACTGGCCTC GGTTTCGTGTGGACCTTGATGTTCGCCTGGTCCATGCCGCACATCACATG CTTGTAGCAGGGTTCGATTTCCTCGATGCGGGTGAGCACGAAGAGCTTGC GACGGCCCTGCTGGTACGAGGGGCTGAATCGCTGGAGTTTGCGATCGCGA CAACCCCGTCGCACTTGAGCTCACCCGACGAAGCACTCAAGGCTGCCGCT GCGTACCACATCGCCGGGCACCACGCCCGGTCATACGTCCTGTCAGAACC ACTGGTGTCGCAAGAGTGGACCGATGCTCTTCCTGCCGGCTTGCTTGCAC TGCTACGGCGAGACTTTGCAGGTCTCGCTAGCCAGCTGCGTGGCCCGGAG TCCTTAGCCATCACTTCTGCTCGGGAGGTTGGGGCACTTCTGCCCGAAGG CGCGATATCGGAAGATGACGCGCTAACGAGAATTGGTCATGACAGCTTAC TCTACGCACTTCGTCTGTATGTAGAGTTTGTCAAACGGGGGCAGAATGCA CTGCTCGATGAAGCCCGTGCTTTGTGTCGGACGGTTGCCGAGCTCGGTCG TGAGTCCCGGCATATCGATTTGTGGTGGTGGGGTCGTGCTACAGAGCACC TACTCCGGGAGCTTGGTGACTCGAGCTTGTGGAGGTGCATCGGCGATCTA GGTCCGGATACTCCCTATGGACGCCCGATCAACCGGTACATCGAAGGGGC CCTAGGAAGCACTCCGTGCACGGTGTCACTGTGGCCGTCGCAGAGGAAGG CCATTGAGATCATCGCAGCTGATGGAGCACCAAGTTTCTGCGTCCGAATG CCAACAAGTGCCGGCAAGACCAAGATCGCGGAACTGGCGATTGTTCGGGC CATTCTTGATGCTGGCTTGGGCGAGAGTGCCAAGTGCCTCTACATTGCGC CGTTCAGATCGCTCGCTGTGGAGATTGAGTCGACCTTGAGCCGAGGACTC AAGCCCCTGGGCATTCGCGTGTCAGAAGTGTACGGCGGATTCGACCTGAC CGCACATGATGAACGCCTCATTGCAGAGACACAGGCACTAATAGCGACGC CCGAGAAGCTTGATGCGGCACTCCGTCTTGCGCCGGAGCTGTTCGACGAT GTCAGACTTGTGGCCATTGATGAGGGCCACATCGCCGGAGACCTATCCGA GCGAGGAATCCGAGCGGAGTTCTTGTTTACTCGCTTGCTTAGGAAGCTCG GTCGTGAACGTTGCCGCCATCTATTCGTGTCCGCGGTACTACCAAATCCG GCTGAGTTCGCAACGTGGATCGCTGGGGACGATGAAAATCTTGTCGACTC TGACTGGAGACCAGCTCGACTCGTACTTGGTGAGTGCCAATGGAACGGCT CGCGACTTCGTATTGAGTTCGATCATGATGTCTCA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig16400:2286..3770+

>mRNA_L-elsbetiae_contig16400.4354.1 ID=mRNA_L-elsbetiae_contig16400.4354.1|Name=mRNA_L-elsbetiae_contig16400.4354.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=2970bp|location=Sequence derived from alignment at L-elsbetiae_contig16400:2286..3770+ (Laminarionema elsbetiae ELsaHSoW15)
CGCGGCATCCTCGCAGACAGACCTCAAGGCAACCTCCATGCACAGGCTCA
CGCGAAACAGATACTCCATGATCTCGACACAGCTGTCGATGACTGGCCTC
GGTTTCGTGTGGACCTTGATGTTCGCCTGGTCCATGCCGCACATCACATG
CTTGTAGCAGGGTTCGATTTCCTCGATGCGGGTGAGCACGAAGAGCTTGC
GACGGCCCTGCTGGTACGAGGGGCTGAATCGCTGGAGTTTGCGATCGCGA
CAACCCCGTCGCACTTGAGCTCACCCGACGAAGCACTCAAGGCTGCCGCT
GCGTACCACATCGCCGGGCACCACGCCCGGTCATACGTCCTGTCAGAACC
ACTGGTGTCGCAAGAGTGGACCGATGCTCTTCCTGCCGGCTTGCTTGCAC
TGCTACGGCGAGACTTTGCAGGTCTCGCTAGCCAGCTGCGTGGCCCGGAG
TCCTTAGCCATCACTTCTGCTCGGGAGGTTGGGGCACTTCTGCCCGAAGG
CGCGATATCGGAAGATGACGCGCTAACGAGAATTGGTCATGACAGCTTAC
TCTACGCACTTCGTCTGTATGTAGAGTTTGTCAAACGGGGGCAGAATGCA
CTGCTCGATGAAGCCCGTGCTTTGTGTCGGACGGTTGCCGAGCTCGGTCG
TGAGTCCCGGCATATCGATTTGTGGTGGTGGGGTCGTGCTACAGAGCACC
TACTCCGGGAGCTTGGTGACTCGAGCTTGTGGAGGTGCATCGGCGATCTA
GGTCCGGATACTCCCTATGGACGCCCGATCAACCGGTACATCGAAGGGGC
CCTAGGAAGCACTCCGTGCACGGTGTCACTGTGGCCGTCGCAGAGGAAGG
CCATTGAGATCATCGCAGCTGATGGAGCACCAAGTTTCTGCGTCCGAATG
CCAACAAGTGCCGGCAAGACCAAGATCGCGGAACTGGCGATTGTTCGGGC
CATTCTTGATGCTGGCTTGGGCGAGAGTGCCAAGTGCCTCTACATTGCGC
CGTTCAGATCGCTCGCTGTGGAGATTGAGTCGACCTTGAGCCGAGGACTC
AAGCCCCTGGGCATTCGCGTGTCAGAAGTGTACGGCGGATTCGACCTGAC
CGCACATGATGAACGCCTCATTGCAGAGACACAGGCACTAATAGCGACGC
CCGAGAAGCTTGATGCGGCACTCCGTCTTGCGCCGGAGCTGTTCGACGAT
GTCAGACTTGTGGCCATTGATGAGGGCCACATCGCCGGAGACCTATCCGA
GCGAGGAATCCGAGCGGAGTTCTTGTTTACTCGCTTGCTTAGGAAGCTCG
GTCGTGAACGTTGCCGCCATCTATTCGTGTCCGCGGTACTACCAAATCCG
GCTGAGTTCGCAACGTGGATCGCTGGGGACGATGAAAATCTTGTCGACTC
TGACTGGAGACCAGCTCGACTCGTACTTGGTGAGTGCCAATGGAACGGCT
CGCGACTTCGTATTGAGTTCGATCATGATGTCTCACGCGGCATCCTCGCA
GACAGACCTCAAGGCAACCTCCATGCACAGGCTCACGCGAAACAGATACT
CCATGATCTCGACACAGCTGTCGATGACTGGCCTCGGTTTCGTGTGGACC
TTGATGTTCGCCTGGTCCATGCCGCACATCACATGCTTGTAGCAGGGTTC
GATTTCCTCGATGCGGGTGAGCACGAAGAGCTTGCGACGGCCCTGCTGGT
ACGAGGGGCTGAATCGCTGGAGTTTGCGATCGCGACAACCCCGTCGCACT
TGAGCTCACCCGACGAAGCACTCAAGGCTGCCGCTGCGTACCACATCGCC
GGGCACCACGCCCGGTCATACGTCCTGTCAGAACCACTGGTGTCGCAAGA
GTGGACCGATGCTCTTCCTGCCGGCTTGCTTGCACTGCTACGGCGAGACT
TTGCAGGTCTCGCTAGCCAGCTGCGTGGCCCGGAGTCCTTAGCCATCACT
TCTGCTCGGGAGGTTGGGGCACTTCTGCCCGAAGGCGCGATATCGGAAGA
TGACGCGCTAACGAGAATTGGTCATGACAGCTTACTCTACGCACTTCGTC
TGTATGTAGAGTTTGTCAAACGGGGGCAGAATGCACTGCTCGATGAAGCC
CGTGCTTTGTGTCGGACGGTTGCCGAGCTCGGTCGTGAGTCCCGGCATAT
CGATTTGTGGTGGTGGGGTCGTGCTACAGAGCACCTACTCCGGGAGCTTG
GTGACTCGAGCTTGTGGAGGTGCATCGGCGATCTAGGTCCGGATACTCCC
TATGGACGCCCGATCAACCGGTACATCGAAGGGGCCCTAGGAAGCACTCC
GTGCACGGTGTCACTGTGGCCGTCGCAGAGGAAGGCCATTGAGATCATCG
CAGCTGATGGAGCACCAAGTTTCTGCGTCCGAATGCCAACAAGTGCCGGC
AAGACCAAGATCGCGGAACTGGCGATTGTTCGGGCCATTCTTGATGCTGG
CTTGGGCGAGAGTGCCAAGTGCCTCTACATTGCGCCGTTCAGATCGCTCG
CTGTGGAGATTGAGTCGACCTTGAGCCGAGGACTCAAGCCCCTGGGCATT
CGCGTGTCAGAAGTGTACGGCGGATTCGACCTGACCGCACATGATGAACG
CCTCATTGCAGAGACACAGGCACTAATAGCGACGCCCGAGAAGCTTGATG
CGGCACTCCGTCTTGCGCCGGAGCTGTTCGACGATGTCAGACTTGTGGCC
ATTGATGAGGGCCACATCGCCGGAGACCTATCCGAGCGAGGAATCCGAGC
GGAGTTCTTGTTTACTCGCTTGCTTAGGAAGCTCGGTCGTGAACGTTGCC
GCCATCTATTCGTGTCCGCGGTACTACCAAATCCGGCTGAGTTCGCAACG
TGGATCGCTGGGGACGATGAAAATCTTGTCGACTCTGACTGGAGACCAGC
TCGACTCGTACTTGGTGAGTGCCAATGGAACGGCTCGCGACTTCGTATTG
AGTTCGATCATGATGTCTCA
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