mRNA_L-elsbetiae_contig16144.4220.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig16144.4220.1
Unique NamemRNA_L-elsbetiae_contig16144.4220.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig16144.4220.1 vs. uniprot
Match: D7FLF6_ECTSI (Putative far-red impaired response protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FLF6_ECTSI)

HSP 1 Score: 302 bits (774), Expect = 1.300e-97
Identity = 145/158 (91.77%), Postives = 152/158 (96.20%), Query Frame = 1
Query:    1 MAQKVSGELWASSDDALQLVAELERGTETVKWELDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNRYNYKLCLYVGVGSENKTQVFAQGFFSNESTEAFDFANKFFLEICGGRPKVIMTDSDAAMKESIAGVFPPPHTTHLLCSWHICKNIKK 474
            M++KV+GELWASS DAL+LVAELERG ETVKWELDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNRYN KLCL+VGV SENKTQVFAQGFFSNESTEAFDFANKFFL+ICGG PKVI+TDSDAAMKESI GVFPPPHTTHLLCSWHICKNIKK
Sbjct:   25 MSRKVTGELWASSGDALELVAELERGNETVKWELDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNRYNNKLCLFVGVDSENKTQVFAQGFFSNESTEAFDFANKFFLDICGGHPKVIITDSDAAMKESIRGVFPPPHTTHLLCSWHICKNIKK 182          
BLAST of mRNA_L-elsbetiae_contig16144.4220.1 vs. uniprot
Match: A0A6H5K454_9PHAE (SWIM-type domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K454_9PHAE)

HSP 1 Score: 212 bits (539), Expect = 1.220e-60
Identity = 107/172 (62.21%), Postives = 127/172 (73.84%), Query Frame = 1
Query:    1 MAQKVSGELWASSDDALQLVAELERGTETVKWELDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNRYNYKLCLYVGVGSENKTQVFAQGFFSNESTEAFDFANKFFLEICGGR--------------PKVIMTDSDAAMKESIAGVFPPPHTTHLLCSWHICKNIKK 474
            M+ KVS   WAS  DAL+LVA+LERG  TVKWE DA ++LRSIMWASPEQI+LARTYGG+VIQDNTCL NRY++KLCL+VGV SENKT +FAQG  SNE   +FDFAN+F LEI GG               P+VI+TD+DAAM  S+A + P  HT HL  SWHI KN+K+
Sbjct:  798 MSDKVSRGRWASKTDALELVADLERGPATVKWESDANHVLRSIMWASPEQIMLARTYGGIVIQDNTCLINRYDFKLCLFVGVDSENKTGIFAQGLLSNEQISSFDFANRFLLEIRGGTQSHHLLPTPPTPHPPQVIITDADAAMTSSVAELMP--HTKHLYSSWHISKNLKR 967          
BLAST of mRNA_L-elsbetiae_contig16144.4220.1 vs. uniprot
Match: A0A6H5JEJ0_9PHAE (SWIM-type domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JEJ0_9PHAE)

HSP 1 Score: 135 bits (341), Expect = 4.090e-34
Identity = 74/193 (38.34%), Postives = 108/193 (55.96%), Query Frame = 1
Query:    7 QKVSGELWASSDDALQLVAELERGTET-VKWELDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNR------------------------------------YNYKLCLYVGVGSENKTQVFAQGFFSNESTEAFDFANKFFLEICGGRPKVIMTDSDAAMKESIAGVFPPPHTTHLLCSWHICKNIKK 474
            QKV  E W++  DAL LV+EL+      VK++ D  + L+ + W++ +Q + AR +GG+V+QDNT LTNR                                    Y     L++GV SENKT VFAQGFFS++S++ + +A K +  ICGG+P+VI+TD+DA M  ++   F P  T HL C WH+ KN++K
Sbjct:   47 QKVQRECWSTPTDALDLVSELKDMAGCYVKYDTDGLHRLKHVFWSTGQQQVFARQWGGIVLQDNTALTNRVLVLIRLIFATGKMSDEHPVRLRFIQPRAFASRSCRYGMHFMLFIGVDSENKTVVFAQGFFSDQSSDTYQWALKHYCTICGGQPEVIITDADAGMTHAVE-TFLPSTTVHLYCLWHVMKNVRK 238          
BLAST of mRNA_L-elsbetiae_contig16144.4220.1 vs. uniprot
Match: UPI0019D552B3 (protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Panicum virgatum TaxID=38727 RepID=UPI0019D552B3)

HSP 1 Score: 95.9 bits (237), Expect = 4.590e-20
Identity = 49/124 (39.52%), Postives = 67/124 (54.03%), Query Frame = 1
Query:  100 LDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNRYNYKLCLYVGVGSENKTQVFAQGFFSNESTEAFDFANKFFLEICGGR-PKVIMTDSDAAMKESIAGVFPPPHTTHLLCSWHICKNI 468
            +D ENI+RSI W      +    YG  +  D T  TNRYN    L VG+    K  +F      +++ E F++  + F+E   G+ PK+IMTD DAAMK++IA   P     H LC WHI KN+
Sbjct:  354 MDEENIVRSIFWTDARGRMDYDLYGDFISFDTTFSTNRYNMPFALIVGINGHGKNVIFGCALIEDQTAETFEWVFRTFVETMSGKKPKIIMTDQDAAMKKAIANYMPD--VIHRLCIWHIMKNL 475          
BLAST of mRNA_L-elsbetiae_contig16144.4220.1 vs. uniprot
Match: UPI000B8AD7C3 (protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Spinacia oleracea TaxID=3562 RepID=UPI000B8AD7C3)

HSP 1 Score: 92.0 bits (227), Expect = 7.180e-19
Identity = 61/158 (38.61%), Postives = 83/158 (52.53%), Query Frame = 1
Query:    1 MAQKVSGELWASSDDALQLVAELERGTETVKWELDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNRYNYKLCLYVGVGSENKTQVFAQGFFSNESTEAFDFANKFFLEICGG--RPKVIMTDSDAAMKESIAGVFPPPHTTHLLCSWHICKNI 468
            +  K+  E   S      L  ELE+G  T  +  D +  L  + +A P  I+LA+TY  V + D T  TN+Y   L   +G+ S NK+   A  F  NE  E + +A K F  I G   +P+VI+TD + A+  +I  VFP   TTHLLC WHI KNI
Sbjct:  157 LKNKIQKESLGSRSLIQALFEELEQGGFTFTYVKDDKGHLTHVFFAHPRSIMLAKTYSSVFVMDCTYKTNKYGMPLLDIIGMTSLNKSFYAAFVFLQNEKEEDYVWALKNFSSILGFNCQPQVIVTDRELALIGAINIVFP--ETTHLLCVWHIQKNI 312          
BLAST of mRNA_L-elsbetiae_contig16144.4220.1 vs. uniprot
Match: UPI000B8AFA28 (protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Spinacia oleracea TaxID=3562 RepID=UPI000B8AFA28)

HSP 1 Score: 92.0 bits (227), Expect = 1.040e-18
Identity = 61/158 (38.61%), Postives = 83/158 (52.53%), Query Frame = 1
Query:    1 MAQKVSGELWASSDDALQLVAELERGTETVKWELDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNRYNYKLCLYVGVGSENKTQVFAQGFFSNESTEAFDFANKFFLEICGG--RPKVIMTDSDAAMKESIAGVFPPPHTTHLLCSWHICKNI 468
            +  K+  E   S      L  ELE+G  T  +  D +  L  + +A P  I+LA+TY  V + D T  TN+Y   L   +G+ S NK+   A  F  NE  E + +A K F  I G   +P+VI+TD + A+  +I  VFP   TTHLLC WHI KNI
Sbjct:  157 LKNKIQKESLGSRSLIQALFEELEQGGFTFTYVKDDKGHLTHVFFAHPRSIMLAKTYSSVFVMDCTYKTNKYGMPLLDIIGMTSLNKSFYAAFVFLQNEKEEDYVWALKNFSSILGFNCQPQVIVTDRELALIGAINIVFP--ETTHLLCVWHIQKNI 312          
BLAST of mRNA_L-elsbetiae_contig16144.4220.1 vs. uniprot
Match: UPI0019D5D764 (protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Panicum virgatum TaxID=38727 RepID=UPI0019D5D764)

HSP 1 Score: 92.0 bits (227), Expect = 1.080e-18
Identity = 48/124 (38.71%), Postives = 66/124 (53.23%), Query Frame = 1
Query:  100 LDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNRYNYKLCLYVGVGSENKTQVFAQGFFSNESTEAFDFANKFFLEICGGR-PKVIMTDSDAAMKESIAGVFPPPHTTHLLCSWHICKNI 468
            +D EN ++SI W      +    YG  +  D T  TN+YN    L VG+    K  +F      +++ E F++  K F+E   G+ PK+IMTD DAAMK++IA   P     H LC WHI KNI
Sbjct:  396 VDEENRVKSIFWTDARGKMDYELYGDFISFDTTFSTNKYNMPFALIVGINGHAKNVIFGCALLEDQTAETFEWVFKTFVETMNGKKPKIIMTDQDAAMKKAIADFMPD--VIHRLCIWHIMKNI 517          
BLAST of mRNA_L-elsbetiae_contig16144.4220.1 vs. uniprot
Match: A0A5J9U8Y8_9POAL (Protein FAR1-RELATED SEQUENCE (Fragment) n=1 Tax=Eragrostis curvula TaxID=38414 RepID=A0A5J9U8Y8_9POAL)

HSP 1 Score: 87.4 bits (215), Expect = 4.400e-17
Identity = 47/126 (37.30%), Postives = 70/126 (55.56%), Query Frame = 1
Query:   97 ELDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNRYNYKLCLYVGVGSENKTQVFAQGFFSNESTEAFDFANKFFLEICGGRPKVIMTDSDAAMKESIAGVFPPPHTTHLLCSWHICKNIKK 474
            +LD  + +RS+ WA     L   ++G VV  D T  TN YN    L+VGV    ++ VFA    + E+T+AF +A + F+++ GG PK I+TD   AM+ +I+   P   T H  C WH+ K  K+
Sbjct:  223 DLDEGSRVRSMFWAHGASKLGYDSFGDVVTFDTTYRTNLYNLPFGLFVGVNHHFQSVVFAAVLLTEETTQAFQWAFRTFVDVMGGEPKTILTDQCQAMRAAISTELP--RTRHRWCRWHVLKKAKE 346          
BLAST of mRNA_L-elsbetiae_contig16144.4220.1 vs. uniprot
Match: UPI001F5C2846 (protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Lolium rigidum TaxID=89674 RepID=UPI001F5C2846)

HSP 1 Score: 87.4 bits (215), Expect = 4.510e-17
Identity = 43/129 (33.33%), Postives = 73/129 (56.59%), Query Frame = 1
Query:   91 KWELDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNRYNYKLCLYVGVGSENKTQVFAQGFFSNESTEAFDFANKFFLEICGGR-PKVIMTDSDAAMKESIAGVFPPPHTTHLLCSWHICKNIKK 474
            K E+D E ++RS+ W   +  +  + YG ++  D T  TN+YN      +G+    KT VF      ++S + F++  + F+E+  G+ PK+I+TD DAA++ +I+ VF      H  C WHI +N+K+
Sbjct:  401 KMEVDGEGMVRSLFWTDAKSRMDYKLYGDLICFDTTYSTNKYNMPFAPIIGINGNAKTIVFGWALLKDQSADTFEWLFESFVEVMEGKKPKLILTDQDAAIQAAISRVFWDAF--HRFCIWHILQNLKQ 527          
BLAST of mRNA_L-elsbetiae_contig16144.4220.1 vs. uniprot
Match: UPI00098AD9DA (protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Aegilops tauschii subsp. strangulata TaxID=200361 RepID=UPI00098AD9DA)

HSP 1 Score: 86.7 bits (213), Expect = 7.350e-17
Identity = 40/129 (31.01%), Postives = 70/129 (54.26%), Query Frame = 1
Query:   91 KWELDAENILRSIMWASPEQILLARTYGGVVIQDNTCLTNRYNYKLCLYVGVGSENKTQVFAQGFFSNESTEAFDFANKFFLEICGGR-PKVIMTDSDAAMKESIAGVFPPPHTTHLLCSWHICKNIKK 474
            K + D+ N + S+ W +    L    +G ++  D T  TN+YN      +GV   ++T VF      +E  + F +  + F+++ GG+ P+V++TD DAAMK+++  VFP     H  C WH+C+  ++
Sbjct:   80 KAQRDSSNAILSLFWTNSRSRLDYLLFGDIISFDTTFSTNKYNMPFAPIIGVNGHSRTIVFGWALLQDEKADTFKWLFETFVDVMGGKKPRVVLTDQDAAMKKAVPKVFPDAF--HRFCIWHVCRKARE 206          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig16144.4220.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FLF6_ECTSI1.300e-9791.77Putative far-red impaired response protein n=1 Tax... [more]
A0A6H5K454_9PHAE1.220e-6062.21SWIM-type domain-containing protein n=1 Tax=Ectoca... [more]
A0A6H5JEJ0_9PHAE4.090e-3438.34SWIM-type domain-containing protein n=1 Tax=Ectoca... [more]
UPI0019D552B34.590e-2039.52protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Panic... [more]
UPI000B8AD7C37.180e-1938.61protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Spina... [more]
UPI000B8AFA281.040e-1838.61protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Spina... [more]
UPI0019D5D7641.080e-1838.71protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Panic... [more]
A0A5J9U8Y8_9POAL4.400e-1737.30Protein FAR1-RELATED SEQUENCE (Fragment) n=1 Tax=E... [more]
UPI001F5C28464.510e-1733.33protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Loliu... [more]
UPI00098AD9DA7.350e-1731.01protein FAR1-RELATED SEQUENCE 5-like n=1 Tax=Aegil... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig16144contigL-elsbetiae_contig16144:2518..3416 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score303.1
Seed ortholog evalue7.4e-80
Seed eggNOG ortholog2880.D7FLF6
Hectar predicted targeting categoryother localisation
EggNOG OGs2CMGX@1,2QQB8@2759
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons3
Model size474
Cds size474
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig16144.4220.1prot_L-elsbetiae_contig16144.4220.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig16144 2518..3416 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815667.0316927-CDS-L-elsbetiae_contig16144:2517..27291622815667.0316927-CDS-L-elsbetiae_contig16144:2517..2729Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16144 2518..2729 +
1691683025.3629503-CDS-L-elsbetiae_contig16144:2517..27291691683025.3629503-CDS-L-elsbetiae_contig16144:2517..2729Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16144 2518..2729 +
1622815667.0444252-CDS-L-elsbetiae_contig16144:2887..30351622815667.0444252-CDS-L-elsbetiae_contig16144:2887..3035Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16144 2888..3035 +
1691683025.3722403-CDS-L-elsbetiae_contig16144:2887..30351691683025.3722403-CDS-L-elsbetiae_contig16144:2887..3035Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16144 2888..3035 +
1622815667.0563655-CDS-L-elsbetiae_contig16144:3302..34161622815667.0563655-CDS-L-elsbetiae_contig16144:3302..3416Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16144 3303..3416 +
1691683025.3805208-CDS-L-elsbetiae_contig16144:3302..34161691683025.3805208-CDS-L-elsbetiae_contig16144:3302..3416Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig16144 3303..3416 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig16144.4220.1

>prot_L-elsbetiae_contig16144.4220.1 ID=prot_L-elsbetiae_contig16144.4220.1|Name=mRNA_L-elsbetiae_contig16144.4220.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=158bp
MAQKVSGELWASSDDALQLVAELERGTETVKWELDAENILRSIMWASPEQ
ILLARTYGGVVIQDNTCLTNRYNYKLCLYVGVGSENKTQVFAQGFFSNES
TEAFDFANKFFLEICGGRPKVIMTDSDAAMKESIAGVFPPPHTTHLLCSW
HICKNIKK
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mRNA from alignment at L-elsbetiae_contig16144:2518..3416+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig16144.4220.1 ID=mRNA_L-elsbetiae_contig16144.4220.1|Name=mRNA_L-elsbetiae_contig16144.4220.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=899bp|location=Sequence derived from alignment at L-elsbetiae_contig16144:2518..3416+ (Laminarionema elsbetiae ELsaHSoW15)
ATGGCGCAAAAGGTGTCAGGCGAGCTGTGGGCTTCGTCCGATGATGCCCT GCAACTCGTGGCAGAGCTGGAACGGGGGACAGAGACGGTGAAGTGGGAGC TAGACGCGGAGAACATCTTGAGGTCCATTATGTGGGCCTCACCTGAACAA ATTCTTCTCGCGAGAACGTATGGTGGCGTTGTCATTCAAGACAACACATG CCTGACGAACAGGTATTGTAATTTTTCGGTAGTACGCCGTCCTTTGGATG TTGGGGAAAGGGTTTTAGGGGCAGTAAAGGCCCCACCGTGAATCATGACG TGCCGTGGCCAAGCAAGAGGATATAACCCTACAAATATTCACCTTTTCTC CCATGCCAATCGTACGACAGGTACAACTATAAGTTGTGTTTGTACGTGGG CGTGGGCTCAGAAAACAAGACGCAGGTTTTTGCGCAAGGTTTCTTCAGCA ACGAATCGACTGAAGCGTTCGATTTCGCCAACAAGTTCTTCCTCGAGATC TGCGGGGGTCGCCCTAAGGTGGGCGTGCTCGTGTTCGTAATCGACTTGCT CCTTGTAGACCCCACTTAACCCTCCAGACACAACGGGGACTCTCGAAACT CGAAAATAAAAAAATGCGCGGAGAACACACAGGGGTCTGGGCGTTTCAGG TTGCGGGGTGTTTTTTTCTGTCGCTAAAAGGTCCATGGACGGAGAGTAGC GTCACGTGTGTGGGGCACTGGCCGTTGGCCGTTGTGGTTGTCATCTCCAT TTCCACACCCCGTCTTACACCCGCCCCCTCCATAGGTGATCATGACAGAT TCCGACGCGGCGATGAAGGAGTCCATCGCAGGTGTGTTTCCCCCACCCCA CACGACGCACTTGCTCTGCTCATGGCACATCTGCAAGAACATCAAGAAG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig16144:2518..3416+

>mRNA_L-elsbetiae_contig16144.4220.1 ID=mRNA_L-elsbetiae_contig16144.4220.1|Name=mRNA_L-elsbetiae_contig16144.4220.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=948bp|location=Sequence derived from alignment at L-elsbetiae_contig16144:2518..3416+ (Laminarionema elsbetiae ELsaHSoW15)
ATGGCGCAAAAGGTGTCAGGCGAGCTGTGGGCTTCGTCCGATGATGCCCT
GCAACTCGTGGCAGAGCTGGAACGGGGGACAGAGACGGTGAAGTGGGAGC
TAGACGCGGAGAACATCTTGAGGTCCATTATGTGGGCCTCACCTGAACAA
ATTCTTCTCGCGAGAACGTATGGTGGCGTTGTCATTCAAGACAACACATG
CCTGACGAACAGATGGCGCAAAAGGTGTCAGGCGAGCTGTGGGCTTCGTC
CGATGATGCCCTGCAACTCGTGGCAGAGCTGGAACGGGGGACAGAGACGG
TGAAGTGGGAGCTAGACGCGGAGAACATCTTGAGGTCCATTATGTGGGCC
TCACCTGAACAAATTCTTCTCGCGAGAACGTATGGTGGCGTTGTCATTCA
AGACAACACATGCCTGACGAACAGGTACAACTATAAGTTGTGTTTGTACG
TGGGCGTGGGCTCAGAAAACAAGACGCAGGTTTTTGCGCAAGGTTTCTTC
AGCAACGAATCGACTGAAGCGTTCGATTTCGCCAACAAGTTCTTCCTCGA
GATCTGCGGGGGTCGCCCTAAGGTACAACTATAAGTTGTGTTTGTACGTG
GGCGTGGGCTCAGAAAACAAGACGCAGGTTTTTGCGCAAGGTTTCTTCAG
CAACGAATCGACTGAAGCGTTCGATTTCGCCAACAAGTTCTTCCTCGAGA
TCTGCGGGGGTCGCCCTAAGGTGATCATGACAGATTCCGACGCGGCGATG
AAGGAGTCCATCGCAGGTGTGTTTCCCCCACCCCACACGACGCACTTGCT
CTGCTCATGGCACATCTGCAAGAACATCAAGAAGGTGATCATGACAGATT
CCGACGCGGCGATGAAGGAGTCCATCGCAGGTGTGTTTCCCCCACCCCAC
ACGACGCACTTGCTCTGCTCATGGCACATCTGCAAGAACATCAAGAAG
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