mRNA_L-elsbetiae_contig15950.4107.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig15950.4107.1
Unique NamemRNA_L-elsbetiae_contig15950.4107.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
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BLAST of mRNA_L-elsbetiae_contig15950.4107.1 vs. uniprot
Match: D7FPE8_ECTSI (Intraflagellar transpot protein 74 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FPE8_ECTSI)

HSP 1 Score: 169 bits (427), Expect = 1.270e-48
Identity = 90/113 (79.65%), Postives = 98/113 (86.73%), Query Frame = 1 
Query:    1 QGMSGMKTAGGGGIG-----RQVQDASFFIGVLRGKMNDVRAEVGRLRTEIERHDKDVSQQGHLERTYESMLQEVKTLEGTLADYNLAVDKARTTADPGEVTKYLHDLEDQNR 324
            QGMSGMKTAG          RQVQD SFFIGVLR KMNDVRAE+GRL+ EI+RH+K VSQQGHLERTY++MLQ+VK LEGTLADYNLAVDKARTTADPGEVTKYLHDLED+NR
Sbjct:   69 QGMSGMKTAGXXXXXXXXXXRQVQDGSFFIGVLRSKMNDVRAELGRLQAEIDRHEKSVSQQGHLERTYDTMLQDVKALEGTLADYNLAVDKARTTADPGEVTKYLHDLEDRNR 181
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BLAST of mRNA_L-elsbetiae_contig15950.4107.1 vs. uniprot
Match: A0A6H5JUN2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JUN2_9PHAE)

HSP 1 Score: 144 bits (364), Expect = 6.810e-39
Identity = 86/144 (59.72%), Postives = 94/144 (65.28%), Query Frame = 1 
Query:    7 MSGMKTAGGGGIG---RQVQDASFFIGVLRGKMNDVRAEVGRLRTEIERHDKDVSQQGHLERTYESMLQE-----------------------------------VKTLEGTLADYNLAVDKARTTADPGEVTKYLHDLEDQNR 324
            MSGMKTA         RQVQD SFFIGVLR KMNDVRAE+GRL+ EI+RH+K VSQQGHLERTY++MLQE                                   VK LEGTLADYNLAVDKARTTADP E+TKYLHDLED+NR
Sbjct:    1 MSGMKTAAXXXXXXXXRQVQDGSFFIGVLRSKMNDVRAELGRLQAEIDRHEKSVSQQGHLERTYDTMLQEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDVKALEGTLADYNLAVDKARTTADPSEITKYLHDLEDRNR 144
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BLAST of mRNA_L-elsbetiae_contig15950.4107.1 vs. uniprot
Match: A0A836CE00_9STRA (Intraflagellar transport protein 74 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CE00_9STRA)

HSP 1 Score: 135 bits (339), Expect = 1.880e-35
Identity = 71/108 (65.74%), Postives = 87/108 (80.56%), Query Frame = 1 
Query:    1 QGMSGMKTAGGGGIGRQVQDASFFIGVLRGKMNDVRAEVGRLRTEIERHDKDVSQQGHLERTYESMLQEVKTLEGTLADYNLAVDKARTTADPGEVTKYLHDLEDQNR 324
            QGMSGM+T+ G G GR VQD SFF+G LR K+ D++ E+ +L+ E E+H KD SQ G LERTYE++L EVK LEGTLAD+NLA+DKARTTADPGEV  YL +LED+NR
Sbjct:   59 QGMSGMRTSQGAGPGRLVQDGSFFLGQLRQKIMDIQRELAKLKAEGEQHTKDSSQYGGLERTYETLLAEVKNLEGTLADHNLAMDKARTTADPGEVVAYLSELEDRNR 166
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BLAST of mRNA_L-elsbetiae_contig15950.4107.1 vs. uniprot
Match: A0A6V2XWW9_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V2XWW9_HETAK)

HSP 1 Score: 111 bits (278), Expect = 2.150e-26
Identity = 61/108 (56.48%), Postives = 76/108 (70.37%), Query Frame = 1 
Query:    1 QGMSGMKTAGGGGIGRQVQDASFFIGVLRGKMNDVRAEVGRLRTEIERHDKDVSQQGHLERTYESMLQEVKTLEGTLADYNLAVDKARTTADPGEVTKYLHDLEDQNR 324
            QGMSGMKTAG  G GRQVQD S++IGVLR K+ ++  E+ +L+ EI +H  D +Q   LER YE +L+EV+ LEGTLADYNLA+DK R   DP EV  Y   L  +NR
Sbjct:   72 QGMSGMKTAG-QGPGRQVQDPSYYIGVLRAKLTEIATEISKLKREINQHQTDSAQYSGLERKYEELLKEVRNLEGTLADYNLAMDKVRMHTDPTEVEAYQKQLAAKNR 178
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BLAST of mRNA_L-elsbetiae_contig15950.4107.1 vs. uniprot
Match: A0A7S2XUU4_9STRA (Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XUU4_9STRA)

HSP 1 Score: 95.5 bits (236), Expect = 2.770e-23
Identity = 50/88 (56.82%), Postives = 65/88 (73.86%), Query Frame = 1 
Query:    1 QGMSGMKTAGGGGIGRQVQDASFFIGVLRGKMNDVRAEVGRLRTEIERHDKDVSQQGHLERTYESMLQEVKTLEGTLADYNLAVDKAR 264
            QGM GM+TAG  G GRQVQD S++IGVLR KM ++  E+ RLR E ++H+ D       ER YE++L+EV+ LEG LADYNLA+DK+R
Sbjct:   24 QGMRGMRTAGPQGPGRQVQDPSYYIGVLRAKMAEITKEIERLRQETDQHNTDQQSYSQYERKYETLLKEVRQLEGNLADYNLAMDKSR 111
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BLAST of mRNA_L-elsbetiae_contig15950.4107.1 vs. uniprot
Match: F0YK18_AURAN (Uncharacterized protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YK18_AURAN)

HSP 1 Score: 94.7 bits (234), Expect = 3.150e-22
Identity = 49/109 (44.95%), Postives = 79/109 (72.48%), Query Frame = 1 
Query:    1 QGMSGMKTAGGGGIGRQVQDASFFIGVLRGKMNDVRAEVGRLRTEIERHDKDVSQQGHLERTYESMLQEVKTLEGTLADYNLAVDKARTTA-DPGEVTKYLHDLEDQNR 324
            QGM GMKTA   G  RQVQD+S+++G+LR K+ ++  E+  L++E ++H++ +++ G L++ +E++L EV+ LEGTLADYNLA+DKAR    DP E+  Y+ +   +N+
Sbjct:   25 QGMRGMKTA--QGPARQVQDSSYYVGILRSKVTELMNEIQSLQSEHKQHERSMNEYGGLQKRHENLLNEVRALEGTLADYNLAMDKARMVGTDPAELNDYITEFRAKNK 131
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BLAST of mRNA_L-elsbetiae_contig15950.4107.1 vs. uniprot
Match: A0A7S2FT10_9STRA (Hypothetical protein (Fragment) n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2FT10_9STRA)

HSP 1 Score: 94.4 bits (233), Expect = 9.330e-22
Identity = 50/108 (46.30%), Postives = 75/108 (69.44%), Query Frame = 1 
Query:    1 QGMSGMKTAGGGGIGRQVQDASFFIGVLRGKMNDVRAEVGRLRTEIERHDKDVSQQGHLERTYESMLQEVKTLEGTLADYNLAVDKARTTADPGEVTKYLHDLEDQNR 324
            QGM GMK++ GGG GR VQD++FF+GVLR K+N++ AE+ +L  E + H  D S    +    E++ +EV+TLEGTLADYN+A+DK R  +D  E+  Y+ + + +N+
Sbjct:   95 QGMRGMKSSAGGG-GRLVQDSTFFVGVLRNKVNEITAEIEKLEGEAKTHMSDQSNHSRMNNKAEALDKEVRTLEGTLADYNIAMDKQRQGSDVAELHGYIREYDARNK 201
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BLAST of mRNA_L-elsbetiae_contig15950.4107.1 vs. uniprot
Match: A0A8K1CE55_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CE55_PYTOL)

HSP 1 Score: 97.4 bits (241), Expect = 2.360e-21
Identity = 55/107 (51.40%), Postives = 72/107 (67.29%), Query Frame = 1 
Query:    1 QGMSGMKTAGGGGIGRQVQDASFFIGVLRGKMNDVRAEVGRLRTEIERHDKDVSQQGHLERTYESMLQEVKTLEGTLADYNLAVDKARTTADPGEVTKYLHDLEDQN 321
            QGM GM+  G  G GRQVQDASFF+G L  K +++  E+ +LR EIE+  KD SQ   LER YE++  EV+ LEG LADYNLA+DK R+  DP E+ +    L ++N
Sbjct:   87 QGMMGMRV-GTAGPGRQVQDASFFMGKLHAKTSEITTEIEKLRKEIEQDAKDKSQYAQLERKYETLAIEVRDLEGQLADYNLAMDKLRSATDPQEIRQVQEQLRNRN 192
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BLAST of mRNA_L-elsbetiae_contig15950.4107.1 vs. uniprot
Match: A0A7S3HDQ6_9STRA (Hypothetical protein (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3HDQ6_9STRA)

HSP 1 Score: 97.1 bits (240), Expect = 2.960e-21
Identity = 53/108 (49.07%), Postives = 77/108 (71.30%), Query Frame = 1 
Query:    1 QGMSGMKTAGGGGIGRQVQDASFFIGVLRGKMNDVRAEVGRLRTEIERHDKDVSQQGHLERTYESMLQEVKTLEGTLADYNLAVDKARTTADPGEVTKYLHDLEDQNR 324
            QG+ GMK A GG  GR VQD+++F+G+LR K+NDV  E  RLR E+++  +D SQ   LE+ YE++++  +TLEG LADYNLA+DK RT+ DP +V +    + ++NR
Sbjct:   65 QGVKGMK-AQGGNTGRLVQDSAYFVGLLRKKINDVNTESMRLRQEMDQQSRDNSQYVQLEKRYENLVKNKETLEGQLADYNLALDKTRTSTDPEDVQQMALHMAEKNR 171
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BLAST of mRNA_L-elsbetiae_contig15950.4107.1 vs. uniprot
Match: A0A8J2SUY1_9STRA (Hypothetical protein n=2 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SUY1_9STRA)

HSP 1 Score: 96.7 bits (239), Expect = 4.200e-21
Identity = 49/108 (45.37%), Postives = 78/108 (72.22%), Query Frame = 1 
Query:    1 QGMSGMKTAGGGGIGRQVQDASFFIGVLRGKMNDVRAEVGRLRTEIERHDKDVSQQGHLERTYESMLQEVKTLEGTLADYNLAVDKARTTADPGEVTKYLHDLEDQNR 324
            QGM GMKT    G  R+VQD+S+++GVLR K+ ++  E+  L+TE ++H++ V++ G L++ ++S+L  V+ LEGTLADYNLA+DKAR + DP E+ +Y+     +N+
Sbjct:   40 QGMRGMKTT--HGPARKVQDSSYYVGVLRNKVTELTTEIYSLQTEHKQHERSVNEHGGLQKHHDSLLNRVRILEGTLADYNLALDKARVSTDPVELDEYISGFRAKNK 145
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig15950.4107.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FPE8_ECTSI1.270e-4879.65Intraflagellar transpot protein 74 n=1 Tax=Ectocar... [more]
A0A6H5JUN2_9PHAE6.810e-3959.72Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A836CE00_9STRA1.880e-3565.74Intraflagellar transport protein 74 n=1 Tax=Tribon... [more]
A0A6V2XWW9_HETAK2.150e-2656.48Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A7S2XUU4_9STRA2.770e-2356.82Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa... [more]
F0YK18_AURAN3.150e-2244.95Uncharacterized protein (Fragment) n=1 Tax=Aureoco... [more]
A0A7S2FT10_9STRA9.330e-2246.30Hypothetical protein (Fragment) n=1 Tax=Florenciel... [more]
A0A8K1CE55_PYTOL2.360e-2151.40Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A7S3HDQ6_9STRA2.960e-2149.07Hypothetical protein (Fragment) n=1 Tax=Spumella e... [more]
A0A8J2SUY1_9STRA4.200e-2145.37Hypothetical protein n=2 Tax=Pelagomonas calceolat... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig15950contigL-elsbetiae_contig15950:2003..2913 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score168.7
Seed ortholog evalue1.5e-39
Seed eggNOG ortholog2880.D7FPE8
Preferred nameIFT74
KEGG koko:K19679
Hectar predicted targeting categoryother localisation
GOsGO:0002064,GO:0003334,GO:0003674,GO:0003682,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005813,GO:0005815,GO:0005856,GO:0005929,GO:0006355,GO:0006357,GO:0006810,GO:0006928,GO:0006996,GO:0007017,GO:0007018,GO:0007154,GO:0007165,GO:0007166,GO:0007219,GO:0007275,GO:0007368,GO:0007389,GO:0007507,GO:0008092,GO:0008150,GO:0008285,GO:0008544,GO:0009799,GO:0009855,GO:0009888,GO:0009889,GO:0009891,GO:0009893,GO:0009913,GO:0009987,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010628,GO:0010970,GO:0015630,GO:0015631,GO:0016043,GO:0019219,GO:0019222,GO:0022607,GO:0023052,GO:0030030,GO:0030031,GO:0030154,GO:0030155,GO:0030216,GO:0030705,GO:0030855,GO:0030990,GO:0030992,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031503,GO:0031514,GO:0032501,GO:0032502,GO:0032991,GO:0033628,GO:0033630,GO:0035735,GO:0042073,GO:0042127,GO:0042995,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043588,GO:0044085,GO:0044422,GO:0044424,GO:0044430,GO:0044441,GO:0044446,GO:0044463,GO:0044464,GO:0044782,GO:0045785,GO:0045893,GO:0045935,GO:0045944,GO:0046907,GO:0048468,GO:0048487,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048731,GO:0048856,GO:0048869,GO:0050678,GO:0050680,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051173,GO:0051179,GO:0051234,GO:0051252,GO:0051254,GO:0051641,GO:0051649,GO:0051716,GO:0060255,GO:0060271,GO:0060429,GO:0065007,GO:0070925,GO:0071840,GO:0072359,GO:0080090,GO:0097542,GO:0099111,GO:0120025,GO:0120031,GO:0120036,GO:0120038,GO:1902680,GO:1903506,GO:1903508,GO:1905515,GO:2000112,GO:2001141
EggNOG free text desc.beta-tubulin binding
EggNOG OGs28JR0@1,2QS4E@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03036
Exons2
Model size327
Cds size321
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig15950.4107.1prot_L-elsbetiae_contig15950.4107.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig15950 2009..2913 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815656.4432132-UTR-L-elsbetiae_contig15950:2002..20081622815656.4432132-UTR-L-elsbetiae_contig15950:2002..2008Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig15950 2003..2008 +
1691683018.5271327-UTR-L-elsbetiae_contig15950:2002..20081691683018.5271327-UTR-L-elsbetiae_contig15950:2002..2008Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig15950 2003..2008 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815656.4592478-CDS-L-elsbetiae_contig15950:2008..22061622815656.4592478-CDS-L-elsbetiae_contig15950:2008..2206Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15950 2009..2206 +
1691683018.5404496-CDS-L-elsbetiae_contig15950:2008..22061691683018.5404496-CDS-L-elsbetiae_contig15950:2008..2206Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15950 2009..2206 +
1622815656.4708536-CDS-L-elsbetiae_contig15950:2790..29131622815656.4708536-CDS-L-elsbetiae_contig15950:2790..2913Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15950 2791..2913 +
1691683018.5509274-CDS-L-elsbetiae_contig15950:2790..29131691683018.5509274-CDS-L-elsbetiae_contig15950:2790..2913Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15950 2791..2913 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig15950.4107.1

>prot_L-elsbetiae_contig15950.4107.1 ID=prot_L-elsbetiae_contig15950.4107.1|Name=mRNA_L-elsbetiae_contig15950.4107.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=107bp
MSGMKTAGGGGIGRQVQDASFFIGVLRGKMNDVRAEVGRLRTEIERHDKD
VSQQGHLERTYESMLQEVKTLEGTLADYNLAVDKARTTADPGEVTKYLHD
LEDQNR*
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mRNA from alignment at L-elsbetiae_contig15950:2003..2913+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig15950.4107.1 ID=mRNA_L-elsbetiae_contig15950.4107.1|Name=mRNA_L-elsbetiae_contig15950.4107.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=911bp|location=Sequence derived from alignment at L-elsbetiae_contig15950:2003..2913+ (Laminarionema elsbetiae ELsaHSoW15)
CAGGGCATGTCGGGCATGAAAACAGCCGGCGGCGGCGGTATCGGTAGGCA
GGTCCAGGACGCGTCTTTTTTCATCGGGGTGCTCCGGGGCAAGATGAACG
ACGTCCGAGCCGAGGTGGGGCGGCTCCGCACGGAGATTGAAAGGCACGAC
AAGGACGTCTCGCAGCAGGGGCACCTGGAGCGCACGTACGAGTCGATGCT
GCAGGTACGTATGTGGGTAGGTAAACCACGGGGGCCCTTTTGGAGGCCAT
TTTCCTAGCGGTCGGTAGCACCAGCAGAGGCTCGTCGTTTGTCGTCTCCC
CGGAACCCAGCCTTTAATTATTGGCAGGTTATCGACACCAACGATGTGGA
GCATGGGTGTGTGCACAGCAGCGGTGTTTTAAGTTTTAAGAACCTCTCAA
GCTGTTGTTGCTCTTGGGCCTATCTGGTGGGCGTAGCGGCAGCACTAACA
CGGAGACGCGACAAGATAAATCTCAAACCGCCGGCGTCGTAGCGGGAACT
CTGCGTCCCGTTTGTGGCGATACGTTCCAGCAGGCTCAAGCTGTTTATGT
AGACGCTACAACAGCAAAACGCGTGGTGAACGGATACATAGATCGAGGTT
TTTTCGATTCCTGCCCCCGAACAGTATCGCAGGAAAAGGCACACTGCACT
GGTGGGGCGGACACCGCGCACAGGCATGTCACCTGCAATAGGCAGCACGT
TAACGTCGACAAGGCCAGAAAACGACAAACCCCCGATCGTTTCTGTGCGT
CTCCCCCTCGTTTTTTTTCTGTTTTTCTACATATGTAGGAGGTCAAGACG
CTGGAGGGTACCCTGGCGGACTACAATCTGGCCGTCGACAAGGCCAGGAC
CACCGCGGACCCCGGGGAGGTGACCAAGTACCTGCACGATCTGGAAGACC
AGAATAGGTGA
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Coding sequence (CDS) from alignment at L-elsbetiae_contig15950:2003..2913+

>mRNA_L-elsbetiae_contig15950.4107.1 ID=mRNA_L-elsbetiae_contig15950.4107.1|Name=mRNA_L-elsbetiae_contig15950.4107.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=642bp|location=Sequence derived from alignment at L-elsbetiae_contig15950:2003..2913+ (Laminarionema elsbetiae ELsaHSoW15)
ATGTCGGGCATGAAAACAGCCGGCGGCGGCGGTATCGGTAGGCAGGTCCA
GGACGCGTCTTTTTTCATCGGGGTGCTCCGGGGCAAGATGAACGACGTCC
GAGCCGAGGTGGGGCGGCTCCGCACGGAGATTGAAAGGCACGACAAGGAC
GTCTCGCAGCAGGGGCACCTGGAGCGCACGTACGAGTCGATGCTGCAGAT
GTCGGGCATGAAAACAGCCGGCGGCGGCGGTATCGGTAGGCAGGTCCAGG
ACGCGTCTTTTTTCATCGGGGTGCTCCGGGGCAAGATGAACGACGTCCGA
GCCGAGGTGGGGCGGCTCCGCACGGAGATTGAAAGGCACGACAAGGACGT
CTCGCAGCAGGGGCACCTGGAGCGCACGTACGAGTCGATGCTGCAGGAGG
TCAAGACGCTGGAGGGTACCCTGGCGGACTACAATCTGGCCGTCGACAAG
GCCAGGACCACCGCGGACCCCGGGGAGGTGACCAAGTACCTGCACGATCT
GGAAGACCAGAATAGGTGAGAGGTCAAGACGCTGGAGGGTACCCTGGCGG
ACTACAATCTGGCCGTCGACAAGGCCAGGACCACCGCGGACCCCGGGGAG
GTGACCAAGTACCTGCACGATCTGGAAGACCAGAATAGGTGA
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