mRNA_L-elsbetiae_contig15726.4001.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig15726.4001.1
Unique NamemRNA_L-elsbetiae_contig15726.4001.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig15726.4001.1 vs. uniprot
Match: D7FRH8_ECTSI (Signal peptide peptidase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FRH8_ECTSI)

HSP 1 Score: 169 bits (427), Expect = 1.350e-45
Identity = 103/161 (63.98%), Postives = 114/161 (70.81%), Query Frame = 1
Query:  133 LNVRDVVDALEMAXXXGTHTASSTYSGVFSYRSWGPGRGCLACVQEVRDAITTFRYGHGRAHSGAASSDATDSAGFTSVDGAK-DGQQQQRRKFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKSGLVGLTGIAQQKLFFRGFFDKYGIKPEIFAREASK 612
            +  RDVVDALEMA             G FSYRSWG G   LACVQEVRDA+T FR          A SDA+ +AG    +GA+ DG+Q  R++FTIAVADTFGEGGPAVGEYFLASAFEKVLVQK+G VGLTG   QKLFFRGF DKYGIKPE+FARE  K
Sbjct:    1 METRDVVDALEMAAKDNKVRG---VLGRFSYRSWGGGY--LACVQEVRDAVTRFRE---------AGSDASATAG----EGAEADGRQ--RKRFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKTGYVGLTGSGGQKLFFRGFLDKYGIKPEVFAREEYK 141          
BLAST of mRNA_L-elsbetiae_contig15726.4001.1 vs. uniprot
Match: A0A7S0QZ39_9CRYP (Hypothetical protein n=1 Tax=Cryptomonas curvata TaxID=233186 RepID=A0A7S0QZ39_9CRYP)

HSP 1 Score: 77.4 bits (189), Expect = 3.690e-13
Identity = 44/114 (38.60%), Postives = 57/114 (50.00%), Query Frame = 1
Query:  253 LACVQEVRDAITTFRYGHGRAHSGAASSDATDSAGFTSVDGAKDGQQQQRRKFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKSGLVGLTGIAQQKLFFRGFFDKYGIKPEIF 594
            LA  QEVR AI  FR                          A+       RKF    ADTFGE G    +Y+LAS+F+K+ +Q SGLVGL G++ Q LFF+ F  K G++ +IF
Sbjct:  105 LATAQEVRKAILDFRE-------------------------AQKALPPANRKFAWVAADTFGEAGQGSTQYYLASSFDKIFMQPSGLVGLIGVSMQSLFFKDFLAKIGVQAQIF 193          
BLAST of mRNA_L-elsbetiae_contig15726.4001.1 vs. uniprot
Match: A0A2E2QB37_9PROT (Protease 4 n=2 Tax=Alphaproteobacteria TaxID=28211 RepID=A0A2E2QB37_9PROT)

HSP 1 Score: 75.1 bits (183), Expect = 3.360e-12
Identity = 34/69 (49.28%), Postives = 46/69 (66.67%), Query Frame = 1
Query:  406 KFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKSGLVGLTGIAQQKLFFRGFFDKYGIKPEIFAREASK 612
            K+T A AD+FGE G  + EYFLA++F++V +Q  G + L G A   L+F+G FDKY + PE F RE  K
Sbjct:  148 KYTYAYADSFGEAGYGMDEYFLAASFDEVWMQPLGSLSLNGFALSNLYFKGLFDKYKVMPEFFQREEFK 216          
BLAST of mRNA_L-elsbetiae_contig15726.4001.1 vs. uniprot
Match: A0A7S1E955_HEMAN (Hypothetical protein (Fragment) n=3 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1E955_HEMAN)

HSP 1 Score: 74.3 bits (181), Expect = 6.060e-12
Identity = 40/115 (34.78%), Postives = 57/115 (49.57%), Query Frame = 1
Query:  253 LACVQEVRDAITTFRYGHGRAHSGAASSDATDSAGFTSVDGAKDGQQQQRRKFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKSGLVGLTGIAQQKLFFRGFFDKYGIKPEIFA 597
            +A +QE+RDAI +FR                               Q + R+  +  ADTFGEGG   G Y+LA+AF+KV +Q +GLVGL GI  +  F +    K G++P+  A
Sbjct:   70 MAKMQEIRDAILSFR-----------------------------SDQAEPRRPAVFAADTFGEGGQGTGHYYLATAFDKVYLQPTGLVGLVGIHSEAFFLKKLLAKIGVQPQFLA 155          
BLAST of mRNA_L-elsbetiae_contig15726.4001.1 vs. uniprot
Match: A0A1H5UZK8_9ACTN (Signal peptide peptidase A. Serine peptidase. MEROPS family S49 n=1 Tax=Thermomonospora echinospora TaxID=1992 RepID=A0A1H5UZK8_9ACTN)

HSP 1 Score: 73.2 bits (178), Expect = 1.500e-11
Identity = 35/71 (49.30%), Postives = 48/71 (67.61%), Query Frame = 1
Query:  406 KFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKSGLVGLTGIAQQKLFFRGFFDKYGIKPEIFAREASKWL 618
            K T+A ++TFGEGG     Y+LASAF++V +Q +G VGLTG+A ++ F RG  DK GI+P   AR   K +
Sbjct:   99 KLTVAWSETFGEGGHGTVPYYLASAFDRVYLQPTGEVGLTGVALEEPFVRGALDKAGIEPRFAARHEYKTM 169          
BLAST of mRNA_L-elsbetiae_contig15726.4001.1 vs. uniprot
Match: A0A2P6TQA9_CHLSO ([Acyl-carrier-protein] S-malonyltransferase n=1 Tax=Chlorella sorokiniana TaxID=3076 RepID=A0A2P6TQA9_CHLSO)

HSP 1 Score: 73.2 bits (178), Expect = 1.720e-11
Identity = 43/125 (34.40%), Postives = 63/125 (50.40%), Query Frame = 1
Query:  253 LACVQEVRDAITTFRYGHGRAHSGAASSDATDSAGFTSVDGAKDGQQQQRRKFTIAVADTFGEGGPA-VGEYFLASAFEKVLVQKSGLVGLTGIAQQKLFFRGFFDKYGIKPEIFAREASKWLSS 624
            LA VQE+R A+  FR                              ++   R  T+A AD FGEGG      ++LASAF+ + +Q +GLV +TG+A Q ++ RGF +++ +KP  FARE  K  +S
Sbjct:  117 LAQVQELRSAVAEFR------------------------------RRAGDRAPTVAYADAFGEGGSGGTAAFYLASAFDHIFLQPTGLVSVTGLAAQGIYARGFLERWSVKPAFFAREEYKNAAS 211          
BLAST of mRNA_L-elsbetiae_contig15726.4001.1 vs. uniprot
Match: A0A7S0YTA2_9CRYP (Hypothetical protein (Fragment) n=1 Tax=Hemiselmis tepida TaxID=464990 RepID=A0A7S0YTA2_9CRYP)

HSP 1 Score: 72.8 bits (177), Expect = 1.780e-11
Identity = 36/115 (31.30%), Postives = 56/115 (48.70%), Query Frame = 1
Query:  253 LACVQEVRDAITTFRYGHGRAHSGAASSDATDSAGFTSVDGAKDGQQQQRRKFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKSGLVGLTGIAQQKLFFRGFFDKYGIKPEIFA 597
            +A +QE+RDA+  FR   G                            ++ R+  +  ADTFGE G   G+Y+LA+AF+K+ +Q SGLVG+ G+  +  F +    K G+ P+  A
Sbjct:   93 MATMQEIRDAVMAFRSAQGG---------------------------EEARRPAVYAADTFGESGQGTGQYYLATAFDKIFIQPSGLVGIIGLHSEAFFLKRMLAKLGVHPQFIA 180          
BLAST of mRNA_L-elsbetiae_contig15726.4001.1 vs. uniprot
Match: A0A372JBQ3_9ACTN (Signal peptide peptidase SppA n=3 Tax=Actinomadura TaxID=1988 RepID=A0A372JBQ3_9ACTN)

HSP 1 Score: 72.4 bits (176), Expect = 2.750e-11
Identity = 33/73 (45.21%), Postives = 50/73 (68.49%), Query Frame = 1
Query:  406 KFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKSGLVGLTGIAQQKLFFRGFFDKYGIKPEIFAREASKWLSS 624
            K+T+A A+TFGEGG     Y+LASAF++V +Q +G VGLTG+A ++ FF G  +K G++P    R   K +++
Sbjct:   99 KYTVAWAETFGEGGRGTVPYYLASAFDRVFLQPTGEVGLTGVALEEPFFAGALEKAGVQPRFAKRYEYKTMAN 171          
BLAST of mRNA_L-elsbetiae_contig15726.4001.1 vs. uniprot
Match: A0A3D9T528_9ACTN (Signal peptide peptidase A n=1 Tax=Thermomonospora umbrina TaxID=111806 RepID=A0A3D9T528_9ACTN)

HSP 1 Score: 72.4 bits (176), Expect = 2.750e-11
Identity = 34/73 (46.58%), Postives = 49/73 (67.12%), Query Frame = 1
Query:  406 KFTIAVADTFGEGGPAVGEYFLASAFEKVLVQKSGLVGLTGIAQQKLFFRGFFDKYGIKPEIFAREASKWLSS 624
            K T+A ++TFG+GG     Y+LAS FE+V +Q SG VGLTG+A ++ F RG  +K GI+P   AR   K +++
Sbjct:   99 KLTVAWSETFGDGGRGTVPYYLASVFERVYLQPSGEVGLTGVALEEPFLRGALEKVGIEPRFGARHEYKTMAN 171          
BLAST of mRNA_L-elsbetiae_contig15726.4001.1 vs. uniprot
Match: A0A8J9RFS0_9CHLO (Protease 4 n=1 Tax=Coccomyxa sp. Obi TaxID=2315456 RepID=A0A8J9RFS0_9CHLO)

HSP 1 Score: 72.4 bits (176), Expect = 2.870e-11
Identity = 44/123 (35.77%), Postives = 62/123 (50.41%), Query Frame = 1
Query:  253 LACVQEVRDAITTFRYGHGRAHSGAASSDATDSAGFTSVDGAKDGQQQQRRKFTIAVADTFGEGGPAVG---EYFLASAFEKVLVQKSGLVGLTGIAQQKLFFRGFFDKYGIKPEIFAREASK 612
            LA +QE+R+A+T FR                              ++ + R  T+A AD FGE G  +G    Y+LASAF+KV +Q SGL+ ++G+A    F R FFD + +KP   ARE  K
Sbjct:  112 LAQIQELRNAVTDFR------------------------------ERAEGRVMTVAYADAFGEAG--IGGTLSYYLASAFDKVYMQPSGLLSMSGLASSTPFLRRFFDSWKVKPHFVAREEYK 202          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig15726.4001.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FRH8_ECTSI1.350e-4563.98Signal peptide peptidase n=2 Tax=Ectocarpus TaxID=... [more]
A0A7S0QZ39_9CRYP3.690e-1338.60Hypothetical protein n=1 Tax=Cryptomonas curvata T... [more]
A0A2E2QB37_9PROT3.360e-1249.28Protease 4 n=2 Tax=Alphaproteobacteria TaxID=28211... [more]
A0A7S1E955_HEMAN6.060e-1234.78Hypothetical protein (Fragment) n=3 Tax=Hemiselmis... [more]
A0A1H5UZK8_9ACTN1.500e-1149.30Signal peptide peptidase A. Serine peptidase. MERO... [more]
A0A2P6TQA9_CHLSO1.720e-1134.40[Acyl-carrier-protein] S-malonyltransferase n=1 Ta... [more]
A0A7S0YTA2_9CRYP1.780e-1131.30Hypothetical protein (Fragment) n=1 Tax=Hemiselmis... [more]
A0A372JBQ3_9ACTN2.750e-1145.21Signal peptide peptidase SppA n=3 Tax=Actinomadura... [more]
A0A3D9T528_9ACTN2.750e-1146.58Signal peptide peptidase A n=1 Tax=Thermomonospora... [more]
A0A8J9RFS0_9CHLO2.870e-1135.77Protease 4 n=1 Tax=Coccomyxa sp. Obi TaxID=2315456... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig15726contigL-elsbetiae_contig15726:1421..3251 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score172.2
Seed ortholog evalue2.6e-40
Seed eggNOG ortholog2880.D7FRH8
EggNOG free text desc.signal peptide processing
EggNOG OGs2QQH5@2759,COG0616@1
COG Functional cat.OU
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Ec32 ortholog descriptionsignal peptide peptidase
Ec32 orthologEc-09_000010.1
Exons4
Model size627
Cds size627
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig15726.4001.1prot_L-elsbetiae_contig15726.4001.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig15726 1421..3251 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815646.0903466-CDS-L-elsbetiae_contig15726:1420..15971622815646.0903466-CDS-L-elsbetiae_contig15726:1420..1597Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15726 1421..1597 -
1691683011.8352315-CDS-L-elsbetiae_contig15726:1420..15971691683011.8352315-CDS-L-elsbetiae_contig15726:1420..1597Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15726 1421..1597 -
1622815646.102183-CDS-L-elsbetiae_contig15726:2130..23651622815646.102183-CDS-L-elsbetiae_contig15726:2130..2365Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15726 2131..2365 -
1691683011.84884-CDS-L-elsbetiae_contig15726:2130..23651691683011.84884-CDS-L-elsbetiae_contig15726:2130..2365Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15726 2131..2365 -
1622815646.1145563-CDS-L-elsbetiae_contig15726:2800..28721622815646.1145563-CDS-L-elsbetiae_contig15726:2800..2872Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15726 2801..2872 -
1691683011.866976-CDS-L-elsbetiae_contig15726:2800..28721691683011.866976-CDS-L-elsbetiae_contig15726:2800..2872Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15726 2801..2872 -
1622815646.1279244-CDS-L-elsbetiae_contig15726:3108..32511622815646.1279244-CDS-L-elsbetiae_contig15726:3108..3251Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15726 3109..3251 -
1691683011.883195-CDS-L-elsbetiae_contig15726:3108..32511691683011.883195-CDS-L-elsbetiae_contig15726:3108..3251Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig15726 3109..3251 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig15726.4001.1

>prot_L-elsbetiae_contig15726.4001.1 ID=prot_L-elsbetiae_contig15726.4001.1|Name=mRNA_L-elsbetiae_contig15726.4001.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=209bp
MKQTHRKKNRLPDGEIWLDMDLSQPLSNAPPGVDLAFLFGDVQPLNVRDV
VDALEMAXXXGTHTASSTYSGVFSYRSWGPGRGCLACVQEVRDAITTFRY
GHGRAHSGAASSDATDSAGFTSVDGAKDGQQQQRRKFTIAVADTFGEGGP
AVGEYFLASAFEKVLVQKSGLVGLTGIAQQKLFFRGFFDKYGIKPEIFAR
EASKWLSS*
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mRNA from alignment at L-elsbetiae_contig15726:1421..3251-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig15726.4001.1 ID=mRNA_L-elsbetiae_contig15726.4001.1|Name=mRNA_L-elsbetiae_contig15726.4001.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1831bp|location=Sequence derived from alignment at L-elsbetiae_contig15726:1421..3251- (Laminarionema elsbetiae ELsaHSoW15)
ATGAAACAGACTCACCGGAAAAAAAACCGGCTCCCCGATGGCGAAATATG GCTCGACATGGATCTCTCCCAGCCCTTGTCGAATGCTCCGCCTGGAGTCG ATCTGGCCTTTTTATTCGGCGATGTGCAGCCTCTCAATGTCAGGTACGTA AATATACCGATATAATGCGTGCATCCAACTGCAATATTTGTGAAGGGTAA CCTGGGCTTGTCCGCTTATCTTGGAATGTGACACGAAATGTTTTGAAGGT ATTTGATGCTCGAACTACCACACTATTTCGGTTCGTTTTTCCGTCTCGCG TAATTCAGTGCCTGGCTGGCACCATACCAGCCCATTCTCACGCCATGTCC TGCTCGTTTTCGATGCTGCGCTATTTTAGGGATGTGGTGGATGCTCTGGA GATGGCGGNNNNNNNNGGTACGCATACAGCAAGCAGCACATACTCCGGTG TGTGGGTGTAGCACGGTGATTTGTCTGTGACGTATGCAGACTATGTAGGT GGTGAGTGGCCTCACCAGCACAAGAAGTAAGGGGGTGCACGAACGCGGGC ACTTAAAACCCTGTAAAAAGCACTAGCTTTGGGCAATGTTGCGCGAAGGT GAGATTCGAAAAAGCATGCGAAGATGTCCAAGCTAACCTCACTGCCTATA ATAAAACATACCGTACCCTAAAACATAACTTTTCCCCTTCCCATTATTGT CTTATTCGTCGTCTCCCCCACTGGCCCCCACCCTCCACTTCGACCAGGTC ACACAGGAGGGGGGGGGCGGACCCCCTCCTGGTCGGGTTTGTTTATTCAC TGGTATCTCACATTTTTCGCGTGTTTGCTCTCTTGTTGGACACGCCCGTG TGATTGTTTTGTTGCTGTTTGACAACACGGAAATAGGTTCAGCTACCGCA GCTGGGGTCCCGGACGGGGGTGCCTGGCGTGTGTGCAGGAGGTGCGCGAC GCCATCACCACGTTCCGATACGGACATGGCCGCGCGCATTCCGGTGCCGC ATCGAGCGATGCCACTGATAGTGCTGGTTTTACCTCTGTAGATGGCGCCA AGGACGGGCAGCAGCAGCAGCGGAGAAAGTTTACCATCGCGGTGGCCGAT ACGTTCGGGGAAGGGGGCCCGGTGAGATGAGGCTTGGACTAAGGGCGTAG ATGCTCTGAACCCTGCACGGAGTGCGTTGCGGCCATGTGTAGATAGCGTG GCGTTACATGTGCTTAAGGTTCCGATGGTGGCTTTGGCGGCCAGGGTCTC TCTCCACTATTAGAACAGCCAGTCTAACAATGAAATATTGCATTTTTGGT GTGTATCGGTGGATATCGGGACACCAGGTTTACGACCAACGTGTTCATGT GCTATCATCTGTCTTCTCTCAATGATACAAGACTTTGGTAGCTCCAGGCT TGATTTGATTTCACCCAAGTTTGGGCACGGGAGACGTTGCATTGTCTTGC AGCTGGACTCACCGGGCGAGCTGCAGGATAGCCTGCAGGCCCGTGAATCT ACGGATGCCGTATGTACACACTTGCGCAGAGACCGCTGATTCGTGTGTGC GAAGTGCCTGAAGAACCTCGCATGTCAGTTGTGTTTTTACGACGGCCGTT TCCCCCTCTATCTCCGCGATTCGGTTGTGGTGTGTCGCACTATCCGGAGA ACAGGCGGTTGGCGAGTACTTCTTGGCTTCCGCTTTCGAGAAGGTTCTGG TACAGAAGTCCGGTCTCGTTGGCCTCACAGGGATCGCCCAACAGAAGCTG TTCTTCAGGGGATTTTTCGACAAGTATGGGATCAAGCCCGAGATCTTTGC TCGCGAGGCGAGTAAATGGCTGAGCAGCTAG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig15726:1421..3251-

>mRNA_L-elsbetiae_contig15726.4001.1 ID=mRNA_L-elsbetiae_contig15726.4001.1|Name=mRNA_L-elsbetiae_contig15726.4001.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=1254bp|location=Sequence derived from alignment at L-elsbetiae_contig15726:1421..3251- (Laminarionema elsbetiae ELsaHSoW15)
ATGAAACAGACTCACCGGAAAAAAAACCGGCTCCCCGATGGCGAAATATG
GCTCGACATGGATCTCTCCCAGCCCTTGTCGAATGCTCCGCCTGGAGTCG
ATCTGGCCTTTTTATTCGGCGATGTGCAGCCTCTCAATGTCAGATGAAAC
AGACTCACCGGAAAAAAAACCGGCTCCCCGATGGCGAAATATGGCTCGAC
ATGGATCTCTCCCAGCCCTTGTCGAATGCTCCGCCTGGAGTCGATCTGGC
CTTTTTATTCGGCGATGTGCAGCCTCTCAATGTCAGGGATGTGGTGGATG
CTCTGGAGATGGCGGNNNNNNNNGGTACGCATACAGCAAGCAGCACATAC
TCCGGTGTGGATGTGGTGGATGCTCTGGAGATGGCGGNNNNNNNNGGTAC
GCATACAGCAAGCAGCACATACTCCGGTGTGTTCAGCTACCGCAGCTGGG
GTCCCGGACGGGGGTGCCTGGCGTGTGTGCAGGAGGTGCGCGACGCCATC
ACCACGTTCCGATACGGACATGGCCGCGCGCATTCCGGTGCCGCATCGAG
CGATGCCACTGATAGTGCTGGTTTTACCTCTGTAGATGGCGCCAAGGACG
GGCAGCAGCAGCAGCGGAGAAAGTTTACCATCGCGGTGGCCGATACGTTC
GGGGAAGGGGGCCCGGTTCAGCTACCGCAGCTGGGGTCCCGGACGGGGGT
GCCTGGCGTGTGTGCAGGAGGTGCGCGACGCCATCACCACGTTCCGATAC
GGACATGGCCGCGCGCATTCCGGTGCCGCATCGAGCGATGCCACTGATAG
TGCTGGTTTTACCTCTGTAGATGGCGCCAAGGACGGGCAGCAGCAGCAGC
GGAGAAAGTTTACCATCGCGGTGGCCGATACGTTCGGGGAAGGGGGCCCG
GCGGTTGGCGAGTACTTCTTGGCTTCCGCTTTCGAGAAGGTTCTGGTACA
GAAGTCCGGTCTCGTTGGCCTCACAGGGATCGCCCAACAGAAGCTGTTCT
TCAGGGGATTTTTCGACAAGTATGGGATCAAGCCCGAGATCTTTGCTCGC
GAGGCGAGTAAATGGCTGAGCAGCTAGGCGGTTGGCGAGTACTTCTTGGC
TTCCGCTTTCGAGAAGGTTCTGGTACAGAAGTCCGGTCTCGTTGGCCTCA
CAGGGATCGCCCAACAGAAGCTGTTCTTCAGGGGATTTTTCGACAAGTAT
GGGATCAAGCCCGAGATCTTTGCTCGCGAGGCGAGTAAATGGCTGAGCAG
CTAG
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