mRNA_L-elsbetiae_contig13643.2773.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig13643.2773.1
Unique NamemRNA_L-elsbetiae_contig13643.2773.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig13643.2773.1 vs. uniprot
Match: A0A6H5K085_9PHAE (MPN domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K085_9PHAE)

HSP 1 Score: 273 bits (699), Expect = 1.320e-82
Identity = 127/131 (96.95%), Postives = 129/131 (98.47%), Query Frame = 1
Query:    4 LPVVQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPG 396
            + + QVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPG
Sbjct: 1698 MDLCQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPG 1828          
BLAST of mRNA_L-elsbetiae_contig13643.2773.1 vs. uniprot
Match: A0A836C932_9STRA (NUC071 domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C932_9STRA)

HSP 1 Score: 261 bits (667), Expect = 2.690e-78
Identity = 117/131 (89.31%), Postives = 125/131 (95.42%), Query Frame = 1
Query:    4 LPVVQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPG 396
            + + QVFDQELDALDIENVQKETIHPRKSYKMNSSCAD LLFA+YKW +SKPSLLHDT D YDGTTSNKYW+D+QLRWGDFDSHDVERYARAK+LDYTTDNMSIYPSPCGVL+AIDLAYNLYSGYGNWFPG
Sbjct: 1637 MDLCQVFDQELDALDIENVQKETIHPRKSYKMNSSCADVLLFASYKWQVSKPSLLHDTSDAYDGTTSNKYWLDIQLRWGDFDSHDVERYARAKYLDYTTDNMSIYPSPCGVLIAIDLAYNLYSGYGNWFPG 1767          
BLAST of mRNA_L-elsbetiae_contig13643.2773.1 vs. uniprot
Match: A0A7S3Y066_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3Y066_HETAK)

HSP 1 Score: 258 bits (660), Expect = 2.340e-77
Identity = 117/132 (88.64%), Postives = 126/132 (95.45%), Query Frame = 1
Query:    4 LPVVQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPGA 399
            + + QVFDQELDAL+IENVQKETIHPRKSYKMNSSCAD LLFAAYKW ++KPSLLHDTKD YDGTT+NKYW+DVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSP GVL+A+DLAYNLYSGYGNWFPGA
Sbjct: 1186 MDLCQVFDQELDALEIENVQKETIHPRKSYKMNSSCADVLLFAAYKWQVAKPSLLHDTKDAYDGTTTNKYWLDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPTGVLLAVDLAYNLYSGYGNWFPGA 1317          
BLAST of mRNA_L-elsbetiae_contig13643.2773.1 vs. uniprot
Match: A0A183EXF4_9BILA (PRP8_domainIV domain-containing protein n=1 Tax=Gongylonema pulchrum TaxID=637853 RepID=A0A183EXF4_9BILA)

HSP 1 Score: 234 bits (598), Expect = 1.310e-76
Identity = 106/131 (80.92%), Postives = 118/131 (90.08%), Query Frame = 1
Query:    4 LPVVQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPG 396
            + + QVFDQELDAL+I+ VQKETIHPRKSYKMNSSCAD LLFA YKW +S+PSLL D+KD  D TT+ KYW+D+QLRWGD+DSHDVERYARAKFLDYTTDNMSIYPSP GVL+AIDLAYNLYS YGNWFPG
Sbjct:    1 MDLCQVFDQELDALEIQTVQKETIHPRKSYKMNSSCADILLFAQYKWHVSRPSLLADSKDVMDNTTTQKYWLDIQLRWGDYDSHDVERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLYSAYGNWFPG 131          
BLAST of mRNA_L-elsbetiae_contig13643.2773.1 vs. uniprot
Match: A0A7S3LI28_9STRA (Hypothetical protein (Fragment) n=1 Tax=Aplanochytrium stocchinoi TaxID=215587 RepID=A0A7S3LI28_9STRA)

HSP 1 Score: 235 bits (600), Expect = 2.310e-76
Identity = 105/131 (80.15%), Postives = 117/131 (89.31%), Query Frame = 1
Query:    4 LPVVQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPG 396
            + V QV DQELD+L+IENVQKETIHPRKSYKMNSSCAD LL A+YKW ISK SLLHD +D Y+  +S KYWIDVQLRWGDFDSHD+ERYARAKFLDYTTDNM++YPSP GV++A DLAYNLYSGYGNWFPG
Sbjct:   26 MDVCQVLDQELDSLEIENVQKETIHPRKSYKMNSSCADVLLLASYKWQISKASLLHDNRDSYESISSTKYWIDVQLRWGDFDSHDIERYARAKFLDYTTDNMTLYPSPTGVIIAFDLAYNLYSGYGNWFPG 156          
BLAST of mRNA_L-elsbetiae_contig13643.2773.1 vs. uniprot
Match: A0A8J2WY90_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2WY90_9STRA)

HSP 1 Score: 254 bits (649), Expect = 7.060e-76
Identity = 115/132 (87.12%), Postives = 124/132 (93.94%), Query Frame = 1
Query:    1 TLPVVQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPG 396
            T+ + QVFDQELDAL++ENVQKETIHPRKSYKMNSSCAD LLFAAYKW ++KPSLLHDTKD YDGTTS+KYW+D+QLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSP G LVAIDLAYNLYSGYG WFPG
Sbjct: 1113 TMDLCQVFDQELDALEVENVQKETIHPRKSYKMNSSCADILLFAAYKWQVAKPSLLHDTKDSYDGTTSSKYWLDIQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPTGALVAIDLAYNLYSGYGAWFPG 1244          
BLAST of mRNA_L-elsbetiae_contig13643.2773.1 vs. uniprot
Match: A0A7S3ZNT2_9STRA (Hypothetical protein n=2 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S3ZNT2_9STRA)

HSP 1 Score: 254 bits (649), Expect = 7.110e-76
Identity = 115/132 (87.12%), Postives = 124/132 (93.94%), Query Frame = 1
Query:    1 TLPVVQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPG 396
            T+ + QVFDQELDAL++ENVQKETIHPRKSYKMNSSCAD LLFAAYKW ++KPSLLHDTKD YDGTTS+KYW+D+QLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSP G LVAIDLAYNLYSGYG WFPG
Sbjct: 1631 TMDLCQVFDQELDALEVENVQKETIHPRKSYKMNSSCADILLFAAYKWQVAKPSLLHDTKDSYDGTTSSKYWLDIQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPTGALVAIDLAYNLYSGYGAWFPG 1762          
BLAST of mRNA_L-elsbetiae_contig13643.2773.1 vs. uniprot
Match: F0YIE3_AURAN (MPN domain-containing protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YIE3_AURAN)

HSP 1 Score: 253 bits (646), Expect = 1.800e-75
Identity = 116/132 (87.88%), Postives = 123/132 (93.18%), Query Frame = 1
Query:    1 TLPVVQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPG 396
            T+ + QVFDQELDAL+IENVQKETIHPRKSYKMNSSCAD LLFAAYKW ++KPSLLHDTKD YDG TS+KYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSP G L+AIDLAYNLYSGYG WFPG
Sbjct: 1627 TMDLCQVFDQELDALEIENVQKETIHPRKSYKMNSSCADILLFAAYKWQVAKPSLLHDTKDAYDGATSSKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPTGALIAIDLAYNLYSGYGAWFPG 1758          
BLAST of mRNA_L-elsbetiae_contig13643.2773.1 vs. uniprot
Match: A0A7S2APL4_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2APL4_9STRA)

HSP 1 Score: 253 bits (646), Expect = 1.810e-75
Identity = 115/131 (87.79%), Postives = 124/131 (94.66%), Query Frame = 1
Query:    4 LPVVQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPG 396
            + + QV DQELDAL+IENVQKETIHPRKSYKMNSSCAD LLFAAYKW ++KP+LLHDTKD YDGTT+NKYWIDVQLRWGDFDSHDVERY+RAKFLDYTTDNMSIYPSP GVL+AIDLAYNLYSGYGNWFPG
Sbjct: 1937 MDLCQVLDQELDALEIENVQKETIHPRKSYKMNSSCADILLFAAYKWQVAKPALLHDTKDAYDGTTTNKYWIDVQLRWGDFDSHDVERYSRAKFLDYTTDNMSIYPSPTGVLLAIDLAYNLYSGYGNWFPG 2067          
BLAST of mRNA_L-elsbetiae_contig13643.2773.1 vs. uniprot
Match: A0A1B6LFS8_9HEMI (Uncharacterized protein (Fragment) n=2 Tax=Ecdysozoa TaxID=1206794 RepID=A0A1B6LFS8_9HEMI)

HSP 1 Score: 238 bits (607), Expect = 2.810e-75
Identity = 108/131 (82.44%), Postives = 119/131 (90.84%), Query Frame = 1
Query:    4 LPVVQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTISKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTTDNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPG 396
            + + QVFDQELDAL+IE VQKETIHPRKSYKMNSSCAD LLFAAYKW +S+PSLL D+KD  D TT+ KYWIDVQLRWGD+DSHD+ERYARAKFLDYTTDNMSIYPSP GVL+AIDLAYNL+S YGNWFPG
Sbjct:   20 MDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADVLLFAAYKWNVSRPSLLADSKDTMDNTTTQKYWIDVQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPG 150          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig13643.2773.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5K085_9PHAE1.320e-8296.95MPN domain-containing protein n=2 Tax=Ectocarpus T... [more]
A0A836C932_9STRA2.690e-7889.31NUC071 domain-containing protein n=1 Tax=Tribonema... [more]
A0A7S3Y066_HETAK2.340e-7788.64Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A183EXF4_9BILA1.310e-7680.92PRP8_domainIV domain-containing protein n=1 Tax=Go... [more]
A0A7S3LI28_9STRA2.310e-7680.15Hypothetical protein (Fragment) n=1 Tax=Aplanochyt... [more]
A0A8J2WY90_9STRA7.060e-7687.12Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
A0A7S3ZNT2_9STRA7.110e-7687.12Hypothetical protein n=2 Tax=Pelagomonas calceolat... [more]
F0YIE3_AURAN1.800e-7587.88MPN domain-containing protein n=1 Tax=Aureococcus ... [more]
A0A7S2APL4_9STRA1.810e-7587.79Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A1B6LFS8_9HEMI2.810e-7582.44Uncharacterized protein (Fragment) n=2 Tax=Ecdysoz... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig13643contigL-elsbetiae_contig13643:3362..4434 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score252.7
Seed ortholog evalue9.7e-65
Seed eggNOG ortholog44056.XP_009040176.1
KEGG koko:K12856
KEGG Pathwayko03040,map03040
KEGG ModuleM00354,M00355
Hectar predicted targeting categoryother localisation
GOsGO:0000244,GO:0000375,GO:0000377,GO:0000386,GO:0000387,GO:0000398,GO:0003674,GO:0003676,GO:0003723,GO:0003824,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005681,GO:0005682,GO:0006139,GO:0006396,GO:0006397,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0008380,GO:0009987,GO:0010467,GO:0016043,GO:0016070,GO:0016071,GO:0017069,GO:0017070,GO:0022607,GO:0022613,GO:0022618,GO:0030532,GO:0030619,GO:0030620,GO:0030623,GO:0032991,GO:0034622,GO:0034641,GO:0036002,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0046483,GO:0065003,GO:0071013,GO:0071704,GO:0071826,GO:0071840,GO:0090304,GO:0097157,GO:0097159,GO:0097525,GO:0120114,GO:0140098,GO:1901360,GO:1901363,GO:1902494,GO:1990904
EggNOG free text desc.U5 snRNA binding
EggNOG OGsCOG5178@1,KOG1795@2759
COG Functional cat.A
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03041
Exons2
Model size399
Cds size399
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815530.581071-CDS-L-elsbetiae_contig13643:3361..35641622815530.581071-CDS-L-elsbetiae_contig13643:3361..3564Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13643 3362..3564 +
1691682943.2950017-CDS-L-elsbetiae_contig13643:3361..35641691682943.2950017-CDS-L-elsbetiae_contig13643:3361..3564Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13643 3362..3564 +
1622815530.5931635-CDS-L-elsbetiae_contig13643:4238..44341622815530.5931635-CDS-L-elsbetiae_contig13643:4238..4434Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13643 4239..4434 +
1691682943.3072484-CDS-L-elsbetiae_contig13643:4238..44341691682943.3072484-CDS-L-elsbetiae_contig13643:4238..4434Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig13643 4239..4434 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig13643.2773.1prot_L-elsbetiae_contig13643.2773.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig13643 3362..4434 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig13643.2773.1

>prot_L-elsbetiae_contig13643.2773.1 ID=prot_L-elsbetiae_contig13643.2773.1|Name=mRNA_L-elsbetiae_contig13643.2773.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=133bp
TLPVVQVFDQELDALDIENVQKETIHPRKSYKMNSSCADALLFAAYKWTI
SKPSLLHDTKDQYDGTTSNKYWIDVQLRWGDFDSHDVERYARAKFLDYTT
DNMSIYPSPCGVLVAIDLAYNLYSGYGNWFPGA
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mRNA from alignment at L-elsbetiae_contig13643:3362..4434+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig13643.2773.1 ID=mRNA_L-elsbetiae_contig13643.2773.1|Name=mRNA_L-elsbetiae_contig13643.2773.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=1073bp|location=Sequence derived from alignment at L-elsbetiae_contig13643:3362..4434+ (Laminarionema elsbetiae ELsaHSoW15)
ACCCTTCCTGTGGTCCAGGTGTTCGACCAAGAGCTCGACGCCCTCGACAT CGAGAACGTGCAGAAGGAGACCATCCACCCCCGAAAGTCGTACAAGATGA ACTCCTCGTGCGCCGACGCGCTCCTGTTCGCGGCCTACAAGTGGACCATC TCCAAGCCGTCTCTGCTGCACGACACCAAGGACCAGTACGACGGCACCAC CAGGTGAGAGGGGGGCAGAGGAGGGGGTGGGGGGTCTGCAGGAGAAGGAA GGGCAGGGTCCTGTGTTTTGGCTTGGGTTACGATAGGTGAAGGTCGGAGG TCGTTGCAGTAAAACGTGGGTCTCCTCTCCACCAGGAGTAGGTGTGTGTT TCTGTGACTGGGTAGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTACT TCGCACTCCTAAATATGTTGTACGTTGTGTTTTGAGAGGACATGTAGGTC AACGGGCTGATTCTTTGACACGGGGAAGTACGGGACAGGGCTACAGCAGT TAGTAGTACCGCGGAGCGTCTTGAGGGGTGTTCACGGGATGAGGCGTGTT TTTGGTTTGTTTGATCCCGGTCACGCGTGCGGGTATGTAAGTGTGGGTGG GTCCCTCTCAACCGTCTTCGCCGAACAGGTTTTCACAACAGGTACCCCAA AAAGCATCCCTCCTCCTCCTCCTCCTCCTCCTCCCCCTCCTCCTTCTCTC TGCTGTTGTCTGCTTGCCTATCTTGTCGTCGTTGCATCCCGACTCATTTT AAACTTTGTTATTTATCTGCTGCGCACGTCTCCTTTTCCCTCGCCCCCTT GCTTCTTTCTCCCCCCGGATGGGCGCCCTCTCTTGTTTTTTTCGTGTTTT TATTTTTTTATATTAACGTCGAATCAGCAACAAGTACTGGATCGACGTCC AGCTGCGGTGGGGAGACTTCGACTCGCACGACGTGGAGCGGTACGCGCGC GCCAAGTTTCTGGACTACACGACGGACAACATGTCCATCTACCCGTCCCC CTGCGGGGTGCTGGTGGCGATCGACCTGGCCTACAACCTCTACTCGGGCT ACGGCAACTGGTTCCCAGGTGCG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig13643:3362..4434+

>mRNA_L-elsbetiae_contig13643.2773.1 ID=mRNA_L-elsbetiae_contig13643.2773.1|Name=mRNA_L-elsbetiae_contig13643.2773.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=798bp|location=Sequence derived from alignment at L-elsbetiae_contig13643:3362..4434+ (Laminarionema elsbetiae ELsaHSoW15)
ACCCTTCCTGTGGTCCAGGTGTTCGACCAAGAGCTCGACGCCCTCGACAT
CGAGAACGTGCAGAAGGAGACCATCCACCCCCGAAAGTCGTACAAGATGA
ACTCCTCGTGCGCCGACGCGCTCCTGTTCGCGGCCTACAAGTGGACCATC
TCCAAGCCGTCTCTGCTGCACGACACCAAGGACCAGTACGACGGCACCAC
CAGACCCTTCCTGTGGTCCAGGTGTTCGACCAAGAGCTCGACGCCCTCGA
CATCGAGAACGTGCAGAAGGAGACCATCCACCCCCGAAAGTCGTACAAGA
TGAACTCCTCGTGCGCCGACGCGCTCCTGTTCGCGGCCTACAAGTGGACC
ATCTCCAAGCCGTCTCTGCTGCACGACACCAAGGACCAGTACGACGGCAC
CACCAGCAACAAGTACTGGATCGACGTCCAGCTGCGGTGGGGAGACTTCG
ACTCGCACGACGTGGAGCGGTACGCGCGCGCCAAGTTTCTGGACTACACG
ACGGACAACATGTCCATCTACCCGTCCCCCTGCGGGGTGCTGGTGGCGAT
CGACCTGGCCTACAACCTCTACTCGGGCTACGGCAACTGGTTCCCAGGTG
CGCAACAAGTACTGGATCGACGTCCAGCTGCGGTGGGGAGACTTCGACTC
GCACGACGTGGAGCGGTACGCGCGCGCCAAGTTTCTGGACTACACGACGG
ACAACATGTCCATCTACCCGTCCCCCTGCGGGGTGCTGGTGGCGATCGAC
CTGGCCTACAACCTCTACTCGGGCTACGGCAACTGGTTCCCAGGTGCG
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