mRNA_L-elsbetiae_contig11481.1302.1 (mRNA) Laminarionema elsbetiae ELsaHSoW15

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_L-elsbetiae_contig11481.1302.1
Unique NamemRNA_L-elsbetiae_contig11481.1302.1
TypemRNA
OrganismLaminarionema elsbetiae ELsaHSoW15 (Laminarionema elsbetiae ELsaHSoW15)
Homology
BLAST of mRNA_L-elsbetiae_contig11481.1302.1 vs. uniprot
Match: D7FY04_ECTSI (Putative regulatory protein cII n=2 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FY04_ECTSI)

HSP 1 Score: 446 bits (1147), Expect = 2.560e-152
Identity = 216/318 (67.92%), Postives = 254/318 (79.87%), Query Frame = 1
Query:    1 QVPGRPHMKNNLKDALAPVLQFAGGGGPLGQWGPADVSKIGLPDGCGDGALFLLPGHEGIVDYDQKVTVAYGTNLMVEAAAPGAWNKLMALTARKCGADIVLVDCSPHRGKLNMHIILTSDYLFVPCAPDTYSHNAIKALPAIMKD*IITRHSARNNATHNELAADVQIPAGNPRILGVSVMRYTQMKSQPARNFAFWMGRIGSALNEISAIIHGMLALEGMAGTRGEPDTQPSILVRVPDFNQLAALSHYYGLPVIALGPPLMGIWDAQTELMIPFSGIIRREQSKKVLNFQRIFKGLLQNIGSKVVNGPFVVDFDD 954
            QVPGRP+MKNN+KDAL PVL+ AG   P+ QWGPADV+KI LP+GC  G LFLLPGHEG+VDY+QKVTVAYGTNL+VEA APGAWNKLMALTAR CGADIVLVDC+PHRGKLNMHIILTSDYLF+PC PDTY HNAIKALP IM D + TR +ARN A+H  LA DVQIP  NPRILG+SV RYT ++S+PA+NF +WM RI S L EIS  +H +  L GMAGT G+PDT PSIL +VP+FNQ +ALSH+YGLPVIALG  LM +WD +   M  F+G  RR+ +KKV+ FQ +FK  L+ IG KV +GP +V  D+
Sbjct:  107 QVPGRPNMKNNIKDALRPVLEPAGSDSPMVQWGPADVTKIPLPEGC-QGGLFLLPGHEGLVDYEQKVTVAYGTNLLVEAIAPGAWNKLMALTARSCGADIVLVDCNPHRGKLNMHIILTSDYLFLPCTPDTYCHNAIKALPFIMADWVTTRFNARNTASHAGLARDVQIPKDNPRILGISVNRYTHIRSKPAKNFRYWMTRIESQLREISQDMHKIQGLAGMAGTPGQPDTWPSILAKVPEFNQFSALSHFYGLPVIALGANLMSVWDEEHAAMRTFTGNPRRDMNKKVIFFQEVFKKFLEEIGRKVQSGPLLVALDE 423          
BLAST of mRNA_L-elsbetiae_contig11481.1302.1 vs. uniprot
Match: D7FY09_ECTSI (Putative regulatory protein cII n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FY09_ECTSI)

HSP 1 Score: 438 bits (1127), Expect = 3.750e-145
Identity = 215/322 (66.77%), Postives = 256/322 (79.50%), Query Frame = 1
Query:    1 QVPGRPHMKNNLKDALAPVLQFAGGGGPLGQWGPADVSKIGLPDGCGDGALFLLPGHEGIVDYDQKVTVAYGTNLMVEAAAPGAWNKLMALTARKCGADIVLVDCSPHRGKLNMHIILTSDYLFVPCAPDTYSHNAIKALPAIMKD*IITRHSARNNATHNELAADVQIPAGNPRILGVSVMRYTQMKSQPARNFAFWMGRIGSALNEISAIIHGMLALEGMAGTRGEPDTQPSILVRVPDFNQLAALSHYYGLPVIALGPPLMGIWDAQTELMIPFSGIIRREQSKKVLNFQRIFKGLLQNIGSKVVNGPFVVDFD--DDV 960
            QVPGRP+MKNN+KDAL PVL+ AG   P+ QWGPADV+KI LP+GC  G LFLLPGHEG+VDY+QKVTVAYGTNL+VEA APGAWNKLMALTAR CGADIVLVDC+PHRGKLNMHIILTSDYLF+PC P+TY HNAIKALP IM D + TR +ARN A+H  LA DVQIP  NPRILG+SV RYT ++S+PA+NF +WM RI + L EIS  +H +  L GMAGT G+PDT PSIL +VP+FNQ +ALSH+YGLPVIALG  LMG+W+ +   +  F+G  RR+ +KKV+ FQ +FK  L+ IG K  +GP +V  D  DDV
Sbjct:  109 QVPGRPNMKNNIKDALRPVLEPAGSDSPMVQWGPADVTKIPLPEGC-QGGLFLLPGHEGLVDYEQKVTVAYGTNLLVEAIAPGAWNKLMALTARSCGADIVLVDCNPHRGKLNMHIILTSDYLFLPCTPETYCHNAIKALPFIMADWVTTRFNARNTASHAGLARDVQIPKDNPRILGISVNRYTHIRSKPAKNFRYWMTRIENQLREISQDMHKIQGLAGMAGTPGQPDTWPSILAKVPEFNQFSALSHFYGLPVIALGANLMGVWNEEHAEVQKFTGNPRRDMNKKVIFFQGVFKKFLEEIGRKGQSGPLLVALDEIDDV 429          
BLAST of mRNA_L-elsbetiae_contig11481.1302.1 vs. uniprot
Match: UPI00156D48D6 (AAA family ATPase n=1 Tax=Dorea formicigenerans TaxID=39486 RepID=UPI00156D48D6)

HSP 1 Score: 102 bits (253), Expect = 4.290e-21
Identity = 79/255 (30.98%), Postives = 117/255 (45.88%), Query Frame = 1
Query:   28 NNLKDALAPVLQFAGGGGPLGQWGPADVSKIGLPDGCGDGALFLLPGHEGIVDYDQKVTVAY----GTNLMVEAAAPGAWNKLMALTARKCGADIVLVDCSPHRGKLNMHIILTSDYLFVPCAPDTYSHNAIKALPAIMKD*IITRHSARNNATHNELAADVQIPAGNPRILGVSVMRYTQMKSQPARNFAFWMGRIGSALN-EISAIIHGMLALEGMAGTRGEPDTQPSILVRVPDFNQLAALSHYYGLPVIAL 777
            +N+K AL PVL     GG L     A   +    +      LFLLPGH    ++D    +A       NLM     PGA+ K++ LTA++   DIVLVD SP     N +I++ SDY  VPCAPD + + AI +L  I     IT    ++N      +A  ++    P  LG+   RY      P + FA W+  I   ++ ++  ++     L    G+    ++    L  + DFN L A S  + +PV  L
Sbjct:   66 SNIKSALDPVL-----GGTLEPLTAAKCVEFENNEN-----LFLLPGHVQFAEFDTTYNIAETMTGSLNLMKNV--PGAFRKMLELTAKEYKIDIVLVDMSPSISATNSNILMQSDYFIVPCAPDYFCYMAINSLLTIFPKWEITYKKMQDNDVFQ--SATYKMKNTPPIFLGILHQRYRPRNGSPVKAFAEWINDINDLVDSKLVPVLREHNMLPKQLGSNNYEESYN--LCNIADFNSLIAQSQEHNVPVFLL 304          
BLAST of mRNA_L-elsbetiae_contig11481.1302.1 vs. uniprot
Match: A0A1C0VAW4_9CYAN (AAA_31 domain-containing protein n=1 Tax=Limnothrix sp. P13C2 TaxID=1880902 RepID=A0A1C0VAW4_9CYAN)

HSP 1 Score: 92.0 bits (227), Expect = 1.900e-17
Identity = 84/301 (27.91%), Postives = 134/301 (44.52%), Query Frame = 1
Query:   28 NNLKDALAPVLQFAGGGGPLGQWGPADVSKIG-LP-DGCGDGALFLLPGHEGIVDYDQKVTVAYGTNLMVEAAA--PGAWNKLMALTARKCGADIVLVDCSPHRGKLNMHIILTSDYLFVPCAPDTYSHNAIKALPAIMKD*IITRHSARNNATHNELAADVQIPAGNPRILGVSVMRYTQMKSQPARNFAFWMGRIGSALNE--ISAIIHGMLAL-EGMAGTRGEPDTQPSILVRVPDFNQLAALSHYYGLPVIALGPPLMGIWDAQTELMIPFSGIIRREQSKKVLNFQRIFKGLLQNI 909
            +N+K  LAP  +            P  +  I  +P +GC +  LFLLPGH    +Y+  +  A   +  ++A    PGA N L+  TA K  AD +L+D S   G +N +++  SD+  +P   D +S  AI +L  ++         A +     E  A    PA   + LGV +     +  +    F  W+ +I  A++E  I A+    + L + +   +G  D     L ++P+FN L ALS  +  PV  L P  +G    QT       GI+ +   KK   F+++F  L   I
Sbjct:   69 SNIKTGLAPAFESQ----------PRAIEAIDCVPVEGCQN--LFLLPGHLSFAEYEVALGFAQELSGSIQALRNIPGAINDLLDKTAEKFNADYILIDMSSSLGAINQNLLAISDFFLIPTTADFFSIMAIDSLVKVLPRWYAWAKVASSLQVLRE--AHYPFPATKLKFLGVIIQNNRIIHGKATTAFQNWIEKIEKAVSEQLIPALQKSNMTLPDYLYREQGVQDNLS--LAKIPNFNNLIALSQEHRTPVYDLTPEQLG----QT-------GIVLKSNQKKQEEFRQMFSDLADKI 342          
BLAST of mRNA_L-elsbetiae_contig11481.1302.1 vs. uniprot
Match: UPI000D8CBAEB (AAA family ATPase n=1 Tax=Morganella morganii TaxID=582 RepID=UPI000D8CBAEB)

HSP 1 Score: 66.6 bits (161), Expect = 1.050e-8
Identity = 58/226 (25.66%), Postives = 91/226 (40.27%), Query Frame = 1
Query:  217 TNLMVEAAAPGAWNKLMALTARKCGADIVLVDCSPHRGKLNMHIILTSDYLFVPCAPDTYSHNAIKALPAIMKD*IITRHSARNNATHNELAADVQIPAGNPRILGVSVMRYTQMKSQPARNFAFWMGRIGSAL-NEISAIIHGMLALEGMAGTRGEPDTQPSILVRVPDFNQLAALSHYYGLPVIALGPPLMGIWDAQTELMIPFSGIIRREQSKKVLNFQRIFK 891
            TNLM    A G +   +   A K   D V +D  P  G L     L  D  F+P APD ++  AI  L  I+   +    +       + +  ++ +  GNP  LG  V  + +        +  WM RI   + N+I  II     L        +   + SI++ +PDFNQLA L    G  V  +      +     E   P+ G        ++ +++R+FK
Sbjct:  131 TNLMRTYVAVGDF---IVRLAEKNKYDYVFIDVGPSSGALTRSFFLACDAFFIPVAPDRFNVQAISTLSHIINRWV----NEHRQVVQSYMEIELPVRPGNPAFLGCIVQAFKKFAGVAKPGYQMWMDRIPMEIDNKIKPIIDENKYLPEFE----DKTPKNSIIIEIPDFNQLAPLMQEVGKAVFNISQQDTALITKSGE---PWRGNNWSSAVGRMNSYKRLFK 342          
BLAST of mRNA_L-elsbetiae_contig11481.1302.1 vs. uniprot
Match: UPI000F605B11 (AAA family ATPase n=1 Tax=Kosakonia sp. CCTCC M2018092 TaxID=2492396 RepID=UPI000F605B11)

HSP 1 Score: 64.7 bits (156), Expect = 4.590e-8
Identity = 55/184 (29.89%), Postives = 75/184 (40.76%), Query Frame = 1
Query:  217 TNLMVEAAAPGAWNKLMALTARKCGADIVLVDCSPHRGKLNMHIILTSDYLFVPCAPDTYSHNAIKALPAIMKD*IITRHSARNNATHNELAADVQIPAGNPRILGVSVMRYTQMKSQPARNFAFWMGRIGSALNEISAIIHGMLALEGMAGTRGEPDTQPSILVRVPDFNQLAALSHYYGLPV 768
            TNLM    A G +   +   A K   D V VD  P  G L     L  D  F+P APD ++  AI  L  I+ D  I  H     A    L   + I  GNP  LG  V  + +        +  WM RI     E+   I  ++  +       +     S+++++PDFNQLA L    G  V
Sbjct:  131 TNLMRTYIAVGDF---LVRIADKHKYDYVFVDVGPSSGALTRSFFLACDAFFIPVAPDRFNVQAISTLSHII-DRWIKEH---RQAVQTYLELGLPIRHGNPAFLGCIVQAFKKFAGVAKPGYQMWMDRIPL---EVEKKIKLVIDEDKYLPEFEQKSHSQSVVIKIPDFNQLAPLMQEVGKAV 304          
BLAST of mRNA_L-elsbetiae_contig11481.1302.1 vs. uniprot
Match: A0A6N7HJG8_9PSEU (AAA family ATPase n=1 Tax=Actinophytocola sp. TaxID=1872138 RepID=A0A6N7HJG8_9PSEU)

HSP 1 Score: 63.2 bits (152), Expect = 1.340e-7
Identity = 53/223 (23.77%), Postives = 95/223 (42.60%), Query Frame = 1
Query:  139 GDGALFLLPGHEGIVDYDQKVTVAYGTNLMVEAAA---PGAWNKLMALTARKCGADIVLVDCSPHRGKLNMHIILTSDYLFVPCAPDTYSHNAIKALPAIMKD*IITRHSARNNATHNELAADVQIPAGNPRILGVSVMRYTQMKSQPARNFAFWMGRI-----GSALNEISAIIHGMLALEGMAGTRGEPDTQPSILVRVPDFNQLAALSHYYGLPVIALGP 783
            GD  L LL G   +  ++  ++ A+   L  +  A     A+ +++   A    AD+VLVD  P  G +N  +++ SD++ +P APD +S   ++ L   +      R        H     D+ +PAG+  ++G  VM +     +P + +  W+  I     GS L + +  +               P+T P  L ++  +  L  L+     P+ AL P
Sbjct:   92 GDDRLVLLAGDLELSSFEDDLSGAWPECLDGKPRAFRVMSAFWRVLNDAASSHEADVVLVDVGPSLGAINRGVLVASDHVVLPVAPDLFSLQGLRNLGPTLAG---WRSGWAERRKHGP--PDLAVPAGDMAVVGYVVMGHAVRLDRPVKAYQRWLAEIPGEFRGSVLRQATEQVPA-------------PETDPYCLAQLKHYRSLMPLAQEARKPMFALKP 296          
The following BLAST results are available for this feature:
BLAST of mRNA_L-elsbetiae_contig11481.1302.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
D7FY04_ECTSI2.560e-15267.92Putative regulatory protein cII n=2 Tax=Ectocarpus... [more]
D7FY09_ECTSI3.750e-14566.77Putative regulatory protein cII n=1 Tax=Ectocarpus... [more]
UPI00156D48D64.290e-2130.98AAA family ATPase n=1 Tax=Dorea formicigenerans Ta... [more]
A0A1C0VAW4_9CYAN1.900e-1727.91AAA_31 domain-containing protein n=1 Tax=Limnothri... [more]
UPI000D8CBAEB1.050e-825.66AAA family ATPase n=1 Tax=Morganella morganii TaxI... [more]
UPI000F605B114.590e-829.89AAA family ATPase n=1 Tax=Kosakonia sp. CCTCC M201... [more]
A0A6N7HJG8_9PSEU1.340e-723.77AAA family ATPase n=1 Tax=Actinophytocola sp. TaxI... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
L-elsbetiae_contig11481contigL-elsbetiae_contig11481:2824..3787 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Laminarionema elsbetiae ELsaHSoW152021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score243.0
Seed ortholog evalue8.1e-62
Seed eggNOG ortholog2880.D7FY04
Hectar predicted targeting categoryother localisation
EggNOG free text desc.AAA domain
EggNOG OGs2CZ0S@1,2S7P2@2759
COG Functional cat.D
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons1
Model size964
Cds size420
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_L-elsbetiae_contig11481.1302.1prot_L-elsbetiae_contig11481.1302.1Laminarionema elsbetiae ELsaHSoW15polypeptideL-elsbetiae_contig11481 2845..3264 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815354.5339296-UTR-L-elsbetiae_contig11481:2823..28441622815354.5339296-UTR-L-elsbetiae_contig11481:2823..2844Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig11481 2824..2844 +
1691682857.084099-UTR-L-elsbetiae_contig11481:2823..28441691682857.084099-UTR-L-elsbetiae_contig11481:2823..2844Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig11481 2824..2844 +
1622815354.5627341-UTR-L-elsbetiae_contig11481:3264..37871622815354.5627341-UTR-L-elsbetiae_contig11481:3264..3787Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig11481 3265..3787 +
1691682857.1047614-UTR-L-elsbetiae_contig11481:3264..37871691682857.1047614-UTR-L-elsbetiae_contig11481:3264..3787Laminarionema elsbetiae ELsaHSoW15UTRL-elsbetiae_contig11481 3265..3787 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815354.5464354-CDS-L-elsbetiae_contig11481:2844..32641622815354.5464354-CDS-L-elsbetiae_contig11481:2844..3264Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig11481 2845..3264 +
1691682857.0965347-CDS-L-elsbetiae_contig11481:2844..32641691682857.0965347-CDS-L-elsbetiae_contig11481:2844..3264Laminarionema elsbetiae ELsaHSoW15CDSL-elsbetiae_contig11481 2845..3264 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_L-elsbetiae_contig11481.1302.1

>prot_L-elsbetiae_contig11481.1302.1 ID=prot_L-elsbetiae_contig11481.1302.1|Name=mRNA_L-elsbetiae_contig11481.1302.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=polypeptide|length=140bp
MKNNLKDALAPVLQFAGGGGPLGQWGPADVSKIGLPDGCGDGALFLLPGH
EGIVDYDQKVTVAYGTNLMVEAAAPGAWNKLMALTARKCGADIVLVDCSP
HRGKLNMHIILTSDYLFVPCAPDTYSHNAIKALPAIMKD*
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mRNA from alignment at L-elsbetiae_contig11481:2824..3787+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_L-elsbetiae_contig11481.1302.1 ID=mRNA_L-elsbetiae_contig11481.1302.1|Name=mRNA_L-elsbetiae_contig11481.1302.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=mRNA|length=964bp|location=Sequence derived from alignment at L-elsbetiae_contig11481:2824..3787+ (Laminarionema elsbetiae ELsaHSoW15)
CAGGTGCCTGGTCGCCCTCACATGAAGAACAATCTTAAAGACGCCCTGGC TCCGGTTCTGCAGTTCGCGGGTGGTGGGGGGCCACTCGGACAGTGGGGCC CGGCGGACGTCTCCAAGATTGGCCTGCCAGACGGCTGCGGTGATGGGGCG CTGTTCCTGCTCCCCGGGCACGAAGGAATTGTCGACTACGATCAAAAGGT CACGGTCGCCTACGGCACTAACCTCATGGTCGAGGCGGCTGCCCCGGGTG CATGGAATAAGCTCATGGCGCTCACTGCCCGCAAGTGCGGGGCAGACATC GTCCTCGTCGACTGCAGCCCTCACAGGGGAAAGCTCAACATGCACATCAT CCTGACGTCCGACTACCTGTTCGTACCGTGCGCTCCGGACACGTACAGCC ACAACGCCATCAAGGCCCTCCCTGCCATCATGAAGGACTAGATTATCACT CGGCACAGCGCCCGCAACAATGCTACTCACAATGAACTGGCCGCGGACGT GCAGATTCCGGCGGGTAACCCTCGGATTTTGGGGGTGTCGGTGATGAGGT ACACCCAGATGAAAAGCCAACCAGCGAGGAACTTCGCGTTCTGGATGGGA AGGATCGGCAGCGCTCTTAATGAAATCAGTGCAATAATACATGGCATGCT GGCTTTGGAGGGTATGGCGGGCACGCGTGGAGAGCCAGACACACAGCCGT CGATCCTCGTGAGGGTGCCCGACTTCAACCAACTCGCCGCTTTGAGCCAC TACTACGGTCTTCCGGTCATCGCCTTGGGTCCGCCTCTGATGGGCATCTG GGACGCGCAGACGGAACTGATGATCCCCTTCAGTGGTATAATAAGGCGGG AACAGAGCAAAAAGGTGCTCAACTTCCAAAGAATTTTCAAGGGGCTTCTC CAAAACATCGGCTCCAAGGTTGTGAACGGGCCGTTCGTCGTCGACTTTGA CGACGACGTCATCG
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Coding sequence (CDS) from alignment at L-elsbetiae_contig11481:2824..3787+

>mRNA_L-elsbetiae_contig11481.1302.1 ID=mRNA_L-elsbetiae_contig11481.1302.1|Name=mRNA_L-elsbetiae_contig11481.1302.1|organism=Laminarionema elsbetiae ELsaHSoW15|type=CDS|length=840bp|location=Sequence derived from alignment at L-elsbetiae_contig11481:2824..3787+ (Laminarionema elsbetiae ELsaHSoW15)
ATGAAGAACAATCTTAAAGACGCCCTGGCTCCGGTTCTGCAGTTCGCGGG
TGGTGGGGGGCCACTCGGACAGTGGGGCCCGGCGGACGTCTCCAAGATTG
GCCTGCCAGACGGCTGCGGTGATGGGGCGCTGTTCCTGCTCCCCGGGCAC
GAAGGAATTGTCGACTACGATCAAAAGGTCACGGTCGCCTACGGCACTAA
CCTCATGGTCGAGGCGGCTGCCCCGGGTGCATGGAATAAGCTCATGGCGC
TCACTGCCCGCAAGTGCGGGGCAGACATCGTCCTCGTCGACTGCAGCCCT
CACAGGGGAAAGCTCAACATGCACATCATCCTGACGTCCGACTACCTGTT
CGTACCGTGCGCTCCGGACACGTACAGCCACAACGCCATCAAGGCCCTCC
CTGCCATCATGAAGGACTAGATGAAGAACAATCTTAAAGACGCCCTGGCT
CCGGTTCTGCAGTTCGCGGGTGGTGGGGGGCCACTCGGACAGTGGGGCCC
GGCGGACGTCTCCAAGATTGGCCTGCCAGACGGCTGCGGTGATGGGGCGC
TGTTCCTGCTCCCCGGGCACGAAGGAATTGTCGACTACGATCAAAAGGTC
ACGGTCGCCTACGGCACTAACCTCATGGTCGAGGCGGCTGCCCCGGGTGC
ATGGAATAAGCTCATGGCGCTCACTGCCCGCAAGTGCGGGGCAGACATCG
TCCTCGTCGACTGCAGCCCTCACAGGGGAAAGCTCAACATGCACATCATC
CTGACGTCCGACTACCTGTTCGTACCGTGCGCTCCGGACACGTACAGCCA
CAACGCCATCAAGGCCCTCCCTGCCATCATGAAGGACTAG
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