mRNA_H-elongata_contig184832.4984.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig184832.4984.1
Unique NamemRNA_H-elongata_contig184832.4984.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig184832.4984.1 vs. uniprot
Match: D8LCE0_ECTSI (Glutamate synthase (NADH/NADPH-dependent), C-terminal part n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LCE0_ECTSI)

HSP 1 Score: 219 bits (558), Expect = 8.970e-64
Identity = 111/123 (90.24%), Postives = 116/123 (94.31%), Query Frame = 1 
Query:    7 VRGLLRFKTESSAAIPLEEVEPVSEVMKRFNTGAMSLGSISRETHETLAQAVNKIGGRSNTGEGGEDVERFGDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKVMK 375
            +RGLLRFK E S +IPLEEVEPV E+MKRFNTGAMSLGSISRETHETLAQAVN+IGGRSNTGEGGED ERF DNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPG+KV K
Sbjct:  615 LRGLLRFKDEPSLSIPLEEVEPVGEIMKRFNTGAMSLGSISRETHETLAQAVNEIGGRSNTGEGGEDAERFTDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGSKVTK 737
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BLAST of mRNA_H-elongata_contig184832.4984.1 vs. uniprot
Match: A0A6H5KZS8_9PHAE (Glutamine amidotransferase type-2 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KZS8_9PHAE)

HSP 1 Score: 216 bits (550), Expect = 1.000e-62
Identity = 109/121 (90.08%), Postives = 114/121 (94.21%), Query Frame = 1 
Query:    7 VRGLLRFKTESSAAIPLEEVEPVSEVMKRFNTGAMSLGSISRETHETLAQAVNKIGGRSNTGEGGEDVERFGDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKV 369
            +RGLLRFK E S +IPLEEVEPV E+MKRFNTGAMSLGSISRETHETLAQAVN+IGGRSNTGEGGED ERF DNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPG+K 
Sbjct:  939 LRGLLRFKDEPSLSIPLEEVEPVGEIMKRFNTGAMSLGSISRETHETLAQAVNEIGGRSNTGEGGEDAERFTDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGSKA 1059
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BLAST of mRNA_H-elongata_contig184832.4984.1 vs. uniprot
Match: A0A835YLR4_9STRA (Glutamine amidotransferase type-2 domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YLR4_9STRA)

HSP 1 Score: 207 bits (526), Expect = 1.910e-59
Identity = 103/123 (83.74%), Postives = 115/123 (93.50%), Query Frame = 1 
Query:    7 VRGLLRFKTESSAAIPLEEVEPVSEVMKRFNTGAMSLGSISRETHETLAQAVNKIGGRSNTGEGGEDVERFGDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKVMK 375
            +RGLLRFK++ +AAIP++EVEP +++MKRF TGAMSLGSISRETHETLA AVN IGGRSNTGEGGED ERF D+RRS+IKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKV +
Sbjct:  902 LRGLLRFKSDPAAAIPIDEVEPATQIMKRFCTGAMSLGSISRETHETLAIAVNTIGGRSNTGEGGEDPERFADSRRSSIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKVTR 1024
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BLAST of mRNA_H-elongata_contig184832.4984.1 vs. uniprot
Match: A0A7S0CFF7_9STRA (Hypothetical protein (Fragment) n=1 Tax=Proboscia inermis TaxID=420281 RepID=A0A7S0CFF7_9STRA)

HSP 1 Score: 195 bits (496), Expect = 6.370e-58
Identity = 99/121 (81.82%), Postives = 110/121 (90.91%), Query Frame = 1 
Query:    7 VRGLLRFKTESSAAIPLEEVEPVSEVMKRFNTGAMSLGSISRETHETLAQAVNKIGGRSNTGEGGEDVERFGDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKV 369
            +RG L+FK + S A+PLEEVEPVS+++KRF TGAMSLGSIS+E HETLA A+N IGGRSNTGEGGED +RF DNRRS+IKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPG KV
Sbjct:  123 LRGQLKFKFDPSQAVPLEEVEPVSDIVKRFVTGAMSLGSISQEAHETLAVAMNSIGGRSNTGEGGEDPKRFLDNRRSSIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGFKV 243
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BLAST of mRNA_H-elongata_contig184832.4984.1 vs. uniprot
Match: K0TIB2_THAOC (Glu_synthase domain-containing protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0TIB2_THAOC)

HSP 1 Score: 189 bits (480), Expect = 2.450e-57
Identity = 94/121 (77.69%), Postives = 109/121 (90.08%), Query Frame = 1 
Query:    7 VRGLLRFKTESSAAIPLEEVEPVSEVMKRFNTGAMSLGSISRETHETLAQAVNKIGGRSNTGEGGEDVERFGDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKV 369
            +RG L+F  + S ++PL+EVEPV +++KRF TGAMSLGSIS+E HETLA+A+N +GGRSNTGEGGED ERF DNRRS+IKQVASGRFGVTS+YLANSDQIQIKMAQGAKPGEGGELPG KV
Sbjct:    5 LRGQLKFTFDPSRSVPLDEVEPVQDIVKRFATGAMSLGSISQEAHETLAKAMNALGGRSNTGEGGEDPERFLDNRRSSIKQVASGRFGVTSHYLANSDQIQIKMAQGAKPGEGGELPGFKV 125
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BLAST of mRNA_H-elongata_contig184832.4984.1 vs. uniprot
Match: A0A7S2V0N1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V0N1_9STRA)

HSP 1 Score: 197 bits (502), Expect = 3.270e-56
Identity = 98/121 (80.99%), Postives = 109/121 (90.08%), Query Frame = 1 
Query:    7 VRGLLRFKTESSAAIPLEEVEPVSEVMKRFNTGAMSLGSISRETHETLAQAVNKIGGRSNTGEGGEDVERFGDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKV 369
            +RGLL+FKTE    +P+EEVE  ++++KRFNTGAMSLGSIS+ETHE LA A+NKIGGRSNTGEGGED  RF DNRRSAIKQVASGRFGVTS+YLANSDQIQIKMAQGAKPGEGGELPG KV
Sbjct:  937 LRGLLKFKTEGVKPVPIEEVESAADIVKRFNTGAMSLGSISQETHEALAMAMNKIGGRSNTGEGGEDPARFEDNRRSAIKQVASGRFGVTSHYLANSDQIQIKMAQGAKPGEGGELPGQKV 1057
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BLAST of mRNA_H-elongata_contig184832.4984.1 vs. uniprot
Match: A0A7S1ZND1_TRICV (Hypothetical protein (Fragment) n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1ZND1_TRICV)

HSP 1 Score: 194 bits (493), Expect = 3.480e-56
Identity = 98/121 (80.99%), Postives = 110/121 (90.91%), Query Frame = 1 
Query:    7 VRGLLRFKTESSAAIPLEEVEPVSEVMKRFNTGAMSLGSISRETHETLAQAVNKIGGRSNTGEGGEDVERFGDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKV 369
            +RG L+FK + S +IPL+EVEPVSE++KRF TGAMSLGSIS+E HETLA A+N +GGRSNTGEGGED +RF DNRRSAIKQVASGRFGVTS+YLANSDQIQIKMAQGAKPGEGGELPG KV
Sbjct:  227 LRGQLKFKFDPSKSIPLDEVEPVSEIVKRFATGAMSLGSISQEAHETLAVAMNSLGGRSNTGEGGEDPKRFTDNRRSAIKQVASGRFGVTSHYLANSDQIQIKMAQGAKPGEGGELPGFKV 347
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BLAST of mRNA_H-elongata_contig184832.4984.1 vs. uniprot
Match: A0A448ZBQ4_9STRA (Glutamine amidotransferase type-2 domain-containing protein n=2 Tax=Pseudo-nitzschia TaxID=41953 RepID=A0A448ZBQ4_9STRA)

HSP 1 Score: 197 bits (500), Expect = 6.060e-56
Identity = 100/121 (82.64%), Postives = 110/121 (90.91%), Query Frame = 1 
Query:    7 VRGLLRFKTESSAAIPLEEVEPVSEVMKRFNTGAMSLGSISRETHETLAQAVNKIGGRSNTGEGGEDVERFGDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKV 369
            +RG L+FK + S AIPLEEVEPVSE++KRF TGAMSLGSIS+E HETLA A+N +GGRSNTGEGGED +RF DNRRS+IKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPG KV
Sbjct:  809 LRGQLKFKYDPSKAIPLEEVEPVSEIVKRFATGAMSLGSISQEAHETLAVAMNTLGGRSNTGEGGEDPKRFTDNRRSSIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGFKV 929
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BLAST of mRNA_H-elongata_contig184832.4984.1 vs. uniprot
Match: A0A482SZM0_9ARCH (Glutamate synthase large subunit n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SZM0_9ARCH)

HSP 1 Score: 195 bits (495), Expect = 1.760e-55
Identity = 96/121 (79.34%), Postives = 110/121 (90.91%), Query Frame = 1 
Query:    7 VRGLLRFKTESSAAIPLEEVEPVSEVMKRFNTGAMSLGSISRETHETLAQAVNKIGGRSNTGEGGEDVERFGDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKV 369
            +RG+L+ K + S +IPL EVEP  E++KRFNTGAMSLGSIS+ETHE+LA A+N+IG RSNTGEGGED +RF DNRRSAIKQVASGRFGVTS+YLANSDQ+QIKMAQGAKPGEGGELPGNKV
Sbjct:  268 LRGVLKLKVDKSKSIPLSEVEPAKEIVKRFNTGAMSLGSISQETHESLAVAMNQIGARSNTGEGGEDSKRFLDNRRSAIKQVASGRFGVTSHYLANSDQVQIKMAQGAKPGEGGELPGNKV 388
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BLAST of mRNA_H-elongata_contig184832.4984.1 vs. uniprot
Match: A0A1E7FRF6_9STRA (Glutamate synthase [NADPH] large chain n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7FRF6_9STRA)

HSP 1 Score: 195 bits (496), Expect = 2.100e-55
Identity = 99/121 (81.82%), Postives = 109/121 (90.08%), Query Frame = 1 
Query:    7 VRGLLRFKTESSAAIPLEEVEPVSEVMKRFNTGAMSLGSISRETHETLAQAVNKIGGRSNTGEGGEDVERFGDNRRSAIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGNKV 369
            +RG L+FK   S AIPL+EVEPVSE++KRF TGAMSLGSIS+E HETLA A+N +GGRSNTGEGGED +RF DNRRS+IKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPG KV
Sbjct:  852 LRGQLKFKFAPSKAIPLDEVEPVSEIVKRFATGAMSLGSISQEAHETLAVAMNTLGGRSNTGEGGEDPKRFADNRRSSIKQVASGRFGVTSNYLANSDQIQIKMAQGAKPGEGGELPGFKV 972
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig184832.4984.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LCE0_ECTSI8.970e-6490.24Glutamate synthase (NADH/NADPH-dependent), C-termi... [more]
A0A6H5KZS8_9PHAE1.000e-6290.08Glutamine amidotransferase type-2 domain-containin... [more]
A0A835YLR4_9STRA1.910e-5983.74Glutamine amidotransferase type-2 domain-containin... [more]
A0A7S0CFF7_9STRA6.370e-5881.82Hypothetical protein (Fragment) n=1 Tax=Proboscia ... [more]
K0TIB2_THAOC2.450e-5777.69Glu_synthase domain-containing protein n=1 Tax=Tha... [more]
A0A7S2V0N1_9STRA3.270e-5680.99Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa... [more]
A0A7S1ZND1_TRICV3.480e-5680.99Hypothetical protein (Fragment) n=1 Tax=Trieres ch... [more]
A0A448ZBQ4_9STRA6.060e-5682.64Glutamine amidotransferase type-2 domain-containin... [more]
A0A482SZM0_9ARCH1.760e-5579.34Glutamate synthase large subunit n=1 Tax=archaeon ... [more]
A0A1E7FRF6_9STRA2.100e-5581.82Glutamate synthase [NADPH] large chain n=1 Tax=Fra... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig184832contigH-elongata_contig184832:264..1180 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score220.3
Seed ortholog evalue5e-55
Seed eggNOG ortholog2880.D8LCE0
Preferred nameGLT1
KEGG rclassRC00006,RC00010,RC02799
KEGG koko:K00264,ko:K17792
KEGG TC3.A.8.1
KEGG ReactionR00093,R00114,R00248
KEGG Pathwayko00250,ko00910,ko01100,ko01110,ko01120,ko01130,ko01230,map00250,map00910,map01100,map01110,map01120,map01130,map01230
GOsGO:0001505,GO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0006082,GO:0006520,GO:0006536,GO:0006537,GO:0006541,GO:0006807,GO:0008150,GO:0008152,GO:0008652,GO:0009058,GO:0009064,GO:0009084,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0009719,GO:0009725,GO:0009735,GO:0009987,GO:0010033,GO:0010035,GO:0010038,GO:0015930,GO:0016040,GO:0016053,GO:0016491,GO:0016638,GO:0016639,GO:0017144,GO:0019676,GO:0019740,GO:0019752,GO:0030447,GO:0032502,GO:0036267,GO:0040007,GO:0042133,GO:0042221,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0043648,GO:0043650,GO:0044182,GO:0044237,GO:0044238,GO:0044249,GO:0044281,GO:0044283,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0045181,GO:0046394,GO:0046686,GO:0048589,GO:0050896,GO:0055114,GO:0060359,GO:0065007,GO:0065008,GO:0070783,GO:0071704,GO:0097054,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901607,GO:1901698
EggNOG free text desc.L-glutamate biosynthetic process
EggNOG OGsCOG0069@1,KOG0399@2759
EC1.4.1.13,1.4.1.14
COG Functional cat.E
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko02000,ko03029
Hectar predicted targeting categoryother localisation
Exons2
Model size378
Cds size378
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930058.0280719-CDS-H-elongata_contig184832:263..4821622930058.0280719-CDS-H-elongata_contig184832:263..482Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig184832 264..482 -
1691679264.4884655-CDS-H-elongata_contig184832:263..4821691679264.4884655-CDS-H-elongata_contig184832:263..482Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig184832 264..482 -
1622930058.0476046-CDS-H-elongata_contig184832:1021..11801622930058.0476046-CDS-H-elongata_contig184832:1021..1180Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig184832 1022..1180 -
1691679264.5138762-CDS-H-elongata_contig184832:1021..11801691679264.5138762-CDS-H-elongata_contig184832:1021..1180Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig184832 1022..1180 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig184832.4984.1prot_H-elongata_contig184832.4984.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig184832 264..1180 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig184832.4984.1

>prot_H-elongata_contig184832.4984.1 ID=prot_H-elongata_contig184832.4984.1|Name=mRNA_H-elongata_contig184832.4984.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=126bp
FFVRGLLRFKTESSAAIPLEEVEPVSEVMKRFNTGAMSLGSISRETHETL
AQAVNKIGGRSNTGEGGEDVERFGDNRRSAIKQVASGRFGVTSNYLANSD
QIQIKMAQGAKPGEGGELPGNKVMK*
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mRNA from alignment at H-elongata_contig184832:264..1180-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig184832.4984.1 ID=mRNA_H-elongata_contig184832.4984.1|Name=mRNA_H-elongata_contig184832.4984.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=917bp|location=Sequence derived from alignment at H-elongata_contig184832:264..1180- (Himanthalia elongata Himel1 dioecious)
TTTTTTGTAAGGGGTCTTTTGCGTTTTAAGACCGAATCTTCCGCTGCCAT
CCCGCTCGAAGAAGTGGAGCCAGTGTCGGAAGTGATGAAGCGCTTCAACA
CCGGGGCCATGTCGCTCGGCTCCATATCTCGTGAGACGCACGAGACCCTC
GCGCAGGCGGTGAGTTGGTGGCGAGTCGAGGGTGGGGGGTTTGATCTCTC
AATGGCTCTTCCGGAATGTTTGTTGGCCCCCCTTCAGCGATTAATCGAGT
ACGCTCTATGTGCGTGTGCGTGTCTGTCCGTGTGTCTGTGTGTGTGCGTA
ATGGAATAGTTTTTGAAACGCAATGATTATAAATATTTGAAGAAAATGCG
ATTGAAAAAGAATACATGTAGCATTTGACAAGGAAAAATAAATAAAAATG
AAGCGGCAAAGAGTTTTTGTTTAAATGAAATATGGTGAGATTTATTAATA
CATTTAAATTAAAATAGAAGTGTGTCTCGTTCTCTTGTCAAAAGCGGGGA
AAAAGTCGCCCATCAAATGTAACATAAAATATTATGGGTAAAAAGTAGCA
CGTGGGGGGGGGGGTATCAATAACATGGTAATATAGAGCTATAGTTAATA
TATATTAAAGCAATTGTGTTCAAAATAATAACTGTTGATAAAATACTTTT
ACGGCTTCTGTTTTTGGTTTTTCGCGTTTTCGTTTTTTGCGTCCTTAGGT
GAACAAAATCGGCGGTCGATCCAACACCGGCGAGGGCGGCGAGGACGTGG
AGCGCTTCGGCGACAACAGGCGATCGGCGATAAAGCAGGTGGCCTCCGGC
CGCTTCGGCGTCACATCGAACTACCTCGCCAACTCCGACCAGATCCAGAT
CAAGATGGCCCAGGGCGCCAAGCCCGGTGAAGGCGGAGAGCTCCCTGGCA
ACAAGGTGATGAAATGA
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Coding sequence (CDS) from alignment at H-elongata_contig184832:264..1180-

>mRNA_H-elongata_contig184832.4984.1 ID=mRNA_H-elongata_contig184832.4984.1|Name=mRNA_H-elongata_contig184832.4984.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=756bp|location=Sequence derived from alignment at H-elongata_contig184832:264..1180- (Himanthalia elongata Himel1 dioecious)
TTTTTTGTAAGGGGTCTTTTGCGTTTTAAGACCGAATCTTCCGCTGCCAT
CCCGCTCGAAGAAGTGGAGCCAGTGTCGGAAGTGATGAAGCGCTTCAACA
CCGGGGCCATGTCGCTCGGCTCCATATCTCGTGAGACGCACGAGACCCTC
GCGCAGGCGTTTTTTGTAAGGGGTCTTTTGCGTTTTAAGACCGAATCTTC
CGCTGCCATCCCGCTCGAAGAAGTGGAGCCAGTGTCGGAAGTGATGAAGC
GCTTCAACACCGGGGCCATGTCGCTCGGCTCCATATCTCGTGAGACGCAC
GAGACCCTCGCGCAGGCGGTGAACAAAATCGGCGGTCGATCCAACACCGG
CGAGGGCGGCGAGGACGTGGAGCGCTTCGGCGACAACAGGCGATCGGCGA
TAAAGCAGGTGGCCTCCGGCCGCTTCGGCGTCACATCGAACTACCTCGCC
AACTCCGACCAGATCCAGATCAAGATGGCCCAGGGCGCCAAGCCCGGTGA
AGGCGGAGAGCTCCCTGGCAACAAGGTGATGAAATGAGTGAACAAAATCG
GCGGTCGATCCAACACCGGCGAGGGCGGCGAGGACGTGGAGCGCTTCGGC
GACAACAGGCGATCGGCGATAAAGCAGGTGGCCTCCGGCCGCTTCGGCGT
CACATCGAACTACCTCGCCAACTCCGACCAGATCCAGATCAAGATGGCCC
AGGGCGCCAAGCCCGGTGAAGGCGGAGAGCTCCCTGGCAACAAGGTGATG
AAATGA
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