mRNA_H-elongata_contig172329.4455.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig172329.4455.1
Unique NamemRNA_H-elongata_contig172329.4455.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig172329.4455.1 vs. uniprot
Match: D7FXU6_ECTSI (Nop domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FXU6_ECTSI)

HSP 1 Score: 241 bits (615), Expect = 7.120e-75
Identity = 128/134 (95.52%), Postives = 132/134 (98.51%), Query Frame = 1 
Query:    1 QVVALSEYRLQLSDYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQANPKHKGKISRVLAAKCALAIRVDALADETDTTIGLESRSKVSL 402
            QVVALSEYRLQLS+YLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQA PK+KGKISRVLAAKCALAIRVDALADETDTTIG+ESRSKV +
Sbjct:  263 QVVALSEYRLQLSEYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQAAPKNKGKISRVLAAKCALAIRVDALADETDTTIGIESRSKVEM 396
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BLAST of mRNA_H-elongata_contig172329.4455.1 vs. uniprot
Match: A0A383VYQ8_TETOB (Nop domain-containing protein n=1 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383VYQ8_TETOB)

HSP 1 Score: 218 bits (555), Expect = 3.070e-69
Identity = 112/132 (84.85%), Postives = 122/132 (92.42%), Query Frame = 1 
Query:    1 QVVALSEYRLQLSDYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQANPKHKGKISRVLAAKCALAIRVDALADETDTTIGLESRSKV 396
            QV+ LSEYR QL DYL+SRM A+APNLT++VGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKH+TPKYGLIYHASLIGQ+ PKHKGKISRVLAAKCALAIRVDAL D TD  +G E+R+KV
Sbjct:   19 QVIELSEYRGQLFDYLKSRMAAVAPNLTVLVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHETPKYGLIYHASLIGQSQPKHKGKISRVLAAKCALAIRVDALGDTTDAAVGTEARAKV 150
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BLAST of mRNA_H-elongata_contig172329.4455.1 vs. uniprot
Match: H3G5X2_PHYRM (Nop domain-containing protein n=1 Tax=Phytophthora ramorum TaxID=164328 RepID=H3G5X2_PHYRM)

HSP 1 Score: 213 bits (541), Expect = 1.510e-67
Identity = 109/132 (82.58%), Postives = 121/132 (91.67%), Query Frame = 1 
Query:    1 QVVALSEYRLQLSDYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQANPKHKGKISRVLAAKCALAIRVDALADETDTTIGLESRSKV 396
            QV++L+EYR QL DYL++RM AIAPNLT+MVGELVGARLIAHAGSL+NLAK PASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQ  PKHKGKISRVLAAK ALA+RVDAL D T+ TIG ++R+KV
Sbjct:    8 QVISLTEYRAQLFDYLKNRMNAIAPNLTVMVGELVGARLIAHAGSLMNLAKHPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQTAPKHKGKISRVLAAKTALAVRVDALGDATEATIGFDNRAKV 139
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BLAST of mRNA_H-elongata_contig172329.4455.1 vs. uniprot
Match: A0A7S1D2T7_CYCTE (Hypothetical protein (Fragment) n=1 Tax=Cyclophora tenuis TaxID=216820 RepID=A0A7S1D2T7_CYCTE)

HSP 1 Score: 209 bits (533), Expect = 4.240e-67
Identity = 110/134 (82.09%), Postives = 126/134 (94.03%), Query Frame = 1 
Query:    1 QVVALSEYRLQLSDYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQANPKHKGKISRVLAAKCALAIRVDALADET--DTTIGLESRSKV 396
            QV++++EYR QL +YLR+RM AIAPNLTI+VGELVGARLI+HAGSL+NLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASL+GQA+PK+KGK+SRVLAAK ALAIRVDAL+D+T  DTTIG E R+KV
Sbjct:   49 QVLSMTEYRSQLWEYLRNRMNAIAPNLTILVGELVGARLISHAGSLLNLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLVGQASPKNKGKMSRVLAAKSALAIRVDALSDDTTPDTTIGYEGRAKV 182
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BLAST of mRNA_H-elongata_contig172329.4455.1 vs. uniprot
Match: A0A7S0IQK4_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Calcidiscus leptoporus TaxID=127549 RepID=A0A7S0IQK4_9EUKA)

HSP 1 Score: 218 bits (554), Expect = 1.670e-66
Identity = 111/132 (84.09%), Postives = 124/132 (93.94%), Query Frame = 1 
Query:    1 QVVALSEYRLQLSDYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQANPKHKGKISRVLAAKCALAIRVDALADETDTTIGLESRSKV 396
            QV++LSEYR QL DYL++RMQAIAPNL++MVGELVGARLIAHAGSL+NLAKQPASTVQILGAEKALFRALK KHDTPKYGLIYHASL+GQA PKHKGKISRVLAAKCAL+IRVDAL + ++ TIG E+RSKV
Sbjct:  279 QVISLSEYRSQLWDYLKNRMQAIAPNLSVMVGELVGARLIAHAGSLMNLAKQPASTVQILGAEKALFRALKAKHDTPKYGLIYHASLVGQAQPKHKGKISRVLAAKCALSIRVDALGESSNATIGFEARSKV 410
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BLAST of mRNA_H-elongata_contig172329.4455.1 vs. uniprot
Match: A0A383VYS5_TETOB (Nop domain-containing protein n=2 Tax=Scenedesmaceae TaxID=3086 RepID=A0A383VYS5_TETOB)

HSP 1 Score: 218 bits (555), Expect = 1.920e-66
Identity = 112/132 (84.85%), Postives = 122/132 (92.42%), Query Frame = 1 
Query:    1 QVVALSEYRLQLSDYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQANPKHKGKISRVLAAKCALAIRVDALADETDTTIGLESRSKV 396
            QV+ LSEYR QL DYL+SRM A+APNLT++VGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKH+TPKYGLIYHASLIGQ+ PKHKGKISRVLAAKCALAIRVDAL D TD  +G E+R+KV
Sbjct:  258 QVIELSEYRGQLFDYLKSRMAAVAPNLTVLVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHETPKYGLIYHASLIGQSQPKHKGKISRVLAAKCALAIRVDALGDTTDAAVGTEARAKV 389
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BLAST of mRNA_H-elongata_contig172329.4455.1 vs. uniprot
Match: A0A662YE82_9STRA (Nop domain-containing protein (Fragment) n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662YE82_9STRA)

HSP 1 Score: 213 bits (541), Expect = 2.570e-66
Identity = 109/132 (82.58%), Postives = 121/132 (91.67%), Query Frame = 1 
Query:    1 QVVALSEYRLQLSDYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQANPKHKGKISRVLAAKCALAIRVDALADETDTTIGLESRSKV 396
            QV++L+EYR QL DYL++RM AIAPNLT+MVGELVGARLIAHAGSL+NLAK PASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQ  PKHKGKISRVLAAK ALA+RVDAL D T+ TIG ++R+KV
Sbjct:   93 QVISLTEYRAQLFDYLKNRMNAIAPNLTVMVGELVGARLIAHAGSLMNLAKHPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQTAPKHKGKISRVLAAKTALAVRVDALGDATEATIGFDNRAKV 224
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BLAST of mRNA_H-elongata_contig172329.4455.1 vs. uniprot
Match: A0A7S0NG10_9EUKA (Hypothetical protein n=1 Tax=Phaeocystis antarctica TaxID=33657 RepID=A0A7S0NG10_9EUKA)

HSP 1 Score: 218 bits (554), Expect = 2.590e-66
Identity = 113/132 (85.61%), Postives = 122/132 (92.42%), Query Frame = 1 
Query:    1 QVVALSEYRLQLSDYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQANPKHKGKISRVLAAKCALAIRVDALADETDTTIGLESRSKV 396
            QVV+LSEYR QL DYLR+RMQAIAPNLT +VGELVGARLIAHAGSL+NLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASL+GQA PKHKGKISRVLAAKCAL+IRVDAL D ++ T+G E R KV
Sbjct:  262 QVVSLSEYRAQLWDYLRNRMQAIAPNLTTVVGELVGARLIAHAGSLMNLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLVGQAEPKHKGKISRVLAAKCALSIRVDALGDTSEPTVGYEGRLKV 393
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BLAST of mRNA_H-elongata_contig172329.4455.1 vs. uniprot
Match: A0A835YZD1_9STRA (Nop domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YZD1_9STRA)

HSP 1 Score: 216 bits (551), Expect = 2.920e-66
Identity = 111/132 (84.09%), Postives = 124/132 (93.94%), Query Frame = 1 
Query:    1 QVVALSEYRLQLSDYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQANPKHKGKISRVLAAKCALAIRVDALADETDTTIGLESRSKV 396
            QVVALSEYR+QL DYL++RM AIAPNLTI+VGELVGARLIAHAGSL+NLAK PAST+QILGAEKALFRALKTKHDTPKYGLIYHASLIGQ  PK+KGK+SRVLAAK ALA+RVDA+ + TDTT+GLESR+KV
Sbjct:  263 QVVALSEYRIQLYDYLKNRMAAIAPNLTILVGELVGARLIAHAGSLLNLAKHPASTIQILGAEKALFRALKTKHDTPKYGLIYHASLIGQTAPKNKGKVSRVLAAKTALAVRVDAMGETTDTTLGLESRAKV 394
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BLAST of mRNA_H-elongata_contig172329.4455.1 vs. uniprot
Match: A0A7S3WLX5_9SPIT (Hypothetical protein (Fragment) n=1 Tax=Strombidinopsis acuminata TaxID=141414 RepID=A0A7S3WLX5_9SPIT)

HSP 1 Score: 214 bits (545), Expect = 1.000e-65
Identity = 109/132 (82.58%), Postives = 123/132 (93.18%), Query Frame = 1 
Query:    1 QVVALSEYRLQLSDYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLAKQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQANPKHKGKISRVLAAKCALAIRVDALADETDTTIGLESRSKV 396
            QV++LSEYR QL DYL++RM AIAPNL++MVGELVGARLIAHAGSL+NLAKQPASTVQILGAEKALFRALK KHDTPKYGLIYHASL+GQA PKHKGKISRVLAAKCAL+IRVDAL + ++ TIGL++R KV
Sbjct:  225 QVISLSEYRAQLFDYLKNRMHAIAPNLSVMVGELVGARLIAHAGSLMNLAKQPASTVQILGAEKALFRALKAKHDTPKYGLIYHASLVGQAQPKHKGKISRVLAAKCALSIRVDALGETSNATIGLDARLKV 356
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig172329.4455.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FXU6_ECTSI7.120e-7595.52Nop domain-containing protein n=2 Tax=Ectocarpus T... [more]
A0A383VYQ8_TETOB3.070e-6984.85Nop domain-containing protein n=1 Tax=Tetradesmus ... [more]
H3G5X2_PHYRM1.510e-6782.58Nop domain-containing protein n=1 Tax=Phytophthora... [more]
A0A7S1D2T7_CYCTE4.240e-6782.09Hypothetical protein (Fragment) n=1 Tax=Cyclophora... [more]
A0A7S0IQK4_9EUKA1.670e-6684.09Hypothetical protein (Fragment) n=1 Tax=Calcidiscu... [more]
A0A383VYS5_TETOB1.920e-6684.85Nop domain-containing protein n=2 Tax=Scenedesmace... [more]
A0A662YE82_9STRA2.570e-6682.58Nop domain-containing protein (Fragment) n=2 Tax=N... [more]
A0A7S0NG10_9EUKA2.590e-6685.61Hypothetical protein n=1 Tax=Phaeocystis antarctic... [more]
A0A835YZD1_9STRA2.920e-6684.09Nop domain-containing protein n=1 Tax=Tribonema mi... [more]
A0A7S3WLX5_9SPIT1.000e-6582.58Hypothetical protein (Fragment) n=1 Tax=Strombidin... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig172329contigH-elongata_contig172329:231..1107 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score242.3
Seed ortholog evalue1.3e-61
Seed eggNOG ortholog2880.D7FXU6
Preferred nameNOP58
KEGG koko:K14565
KEGG Pathwayko03008,map03008
GOsGO:0000154,GO:0000447,GO:0000460,GO:0000462,GO:0000466,GO:0000469,GO:0000472,GO:0000478,GO:0000479,GO:0000480,GO:0000966,GO:0000967,GO:0001085,GO:0001091,GO:0001094,GO:0001098,GO:0001099,GO:0001650,GO:0003674,GO:0003676,GO:0003723,GO:0003729,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005730,GO:0005732,GO:0005737,GO:0005829,GO:0006139,GO:0006364,GO:0006396,GO:0006403,GO:0006725,GO:0006807,GO:0008134,GO:0008150,GO:0008152,GO:0009451,GO:0009987,GO:0010467,GO:0015030,GO:0016049,GO:0016070,GO:0016072,GO:0016604,GO:0019899,GO:0022613,GO:0030490,GO:0030515,GO:0030684,GO:0030686,GO:0031428,GO:0031974,GO:0031981,GO:0032040,GO:0032991,GO:0033036,GO:0034470,GO:0034471,GO:0034641,GO:0034660,GO:0040007,GO:0042254,GO:0042274,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043412,GO:0044085,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044452,GO:0044464,GO:0046483,GO:0048254,GO:0051117,GO:0051179,GO:0070013,GO:0070761,GO:0071704,GO:0071840,GO:0090304,GO:0090305,GO:0090501,GO:0090502,GO:0097159,GO:1901360,GO:1901363,GO:1990904
EggNOG free text desc.endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
EggNOG OGsCOG1498@1,KOG2572@2759
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03009
Hectar predicted targeting categoryother localisation
Exons2
Model size402
Cds size402
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930003.554053-CDS-H-elongata_contig172329:230..4491622930003.554053-CDS-H-elongata_contig172329:230..449Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig172329 231..449 +
1691679241.847814-CDS-H-elongata_contig172329:230..4491691679241.847814-CDS-H-elongata_contig172329:230..449Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig172329 231..449 +
1622930003.6023855-CDS-H-elongata_contig172329:924..11071622930003.6023855-CDS-H-elongata_contig172329:924..1107Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig172329 925..1107 +
1691679241.8602667-CDS-H-elongata_contig172329:924..11071691679241.8602667-CDS-H-elongata_contig172329:924..1107Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig172329 925..1107 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig172329.4455.1prot_H-elongata_contig172329.4455.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig172329 231..1107 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig172329.4455.1

>prot_H-elongata_contig172329.4455.1 ID=prot_H-elongata_contig172329.4455.1|Name=mRNA_H-elongata_contig172329.4455.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=134bp
QVVALSEYRLQLSDYLRSRMQAIAPNLTIMVGELVGARLIAHAGSLINLA
KQPASTVQILGAEKALFRALKTKHDTPKYGLIYHASLIGQANPKHKGKIS
RVLAAKCALAIRVDALADETDTTIGLESRSKVSL
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mRNA from alignment at H-elongata_contig172329:231..1107+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig172329.4455.1 ID=mRNA_H-elongata_contig172329.4455.1|Name=mRNA_H-elongata_contig172329.4455.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=877bp|location=Sequence derived from alignment at H-elongata_contig172329:231..1107+ (Himanthalia elongata Himel1 dioecious)
CAGGTGGTGGCGCTGTCGGAGTACCGGCTGCAGCTGTCCGACTACCTTCG
CAGCCGCATGCAGGCCATCGCACCGAACCTAACCATCATGGTGGGCGAGC
TCGTGGGAGCGCGTTTAATCGCACATGCCGGGTCGCTGATCAACCTGGCG
AAGCAGCCGGCGTCCACGGTGCAGATCCTCGGCGCCGAGAAGGCTTTGTT
CCGCGCCCTTAAGACCAAGGTGCGCATGAAAGATGGGGGTCGGGGGAGTT
CCAATCATTCCCTTCCTTTACTTTTCCCCCGTAGAGGTTATGACGCGCAT
GGCGGCGATGTATTCATAGTGGTGTTTGTGAAAATGTGTGTGATGGGGAT
GATGGATGATGTATGATGATCGTGTGTGGTAATTGTGTTACGAGGGAAAT
ATGAAATATCTTGCTTGCGGTATGGTATATAGACGGATGGCTCCCGCGGT
CGCCCACCGAATTTTCTTTATATTAATATTATTTTATTATTACTAATATT
ATTTATTATTATTTTATTTATTTATTTTAACTTGGTTATTTATTATTGCT
GTTGTTATCATTATTATTATTATTACTATTATTATTATTTATTATTTTTT
AGCTACAATAATAATTAAATAAAAATAATCCACCATATATTAATACTAGA
CACTAACGAAGTAATACGTGGCATCGGCGGAACCTCCCTGGCAGCACGAT
ACTCCCAAGTACGGGCTGATCTACCACGCGAGCCTGATTGGCCAGGCTAA
CCCAAAGCACAAGGGTAAGATCTCGCGTGTGTTGGCGGCGAAGTGCGCCC
TGGCGATCCGTGTGGACGCTCTCGCCGACGAGACGGACACCACCATCGGC
CTGGAAAGCCGCTCCAAGGTAAGCCTT
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Coding sequence (CDS) from alignment at H-elongata_contig172329:231..1107+

>mRNA_H-elongata_contig172329.4455.1 ID=mRNA_H-elongata_contig172329.4455.1|Name=mRNA_H-elongata_contig172329.4455.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=804bp|location=Sequence derived from alignment at H-elongata_contig172329:231..1107+ (Himanthalia elongata Himel1 dioecious)
CAGGTGGTGGCGCTGTCGGAGTACCGGCTGCAGCTGTCCGACTACCTTCG
CAGCCGCATGCAGGCCATCGCACCGAACCTAACCATCATGGTGGGCGAGC
TCGTGGGAGCGCGTTTAATCGCACATGCCGGGTCGCTGATCAACCTGGCG
AAGCAGCCGGCGTCCACGGTGCAGATCCTCGGCGCCGAGAAGGCTTTGTT
CCGCGCCCTTAAGACCAAGCAGGTGGTGGCGCTGTCGGAGTACCGGCTGC
AGCTGTCCGACTACCTTCGCAGCCGCATGCAGGCCATCGCACCGAACCTA
ACCATCATGGTGGGCGAGCTCGTGGGAGCGCGTTTAATCGCACATGCCGG
GTCGCTGATCAACCTGGCGAAGCAGCCGGCGTCCACGGTGCAGATCCTCG
GCGCCGAGAAGGCTTTGTTCCGCGCCCTTAAGACCAAGCACGATACTCCC
AAGTACGGGCTGATCTACCACGCGAGCCTGATTGGCCAGGCTAACCCAAA
GCACAAGGGTAAGATCTCGCGTGTGTTGGCGGCGAAGTGCGCCCTGGCGA
TCCGTGTGGACGCTCTCGCCGACGAGACGGACACCACCATCGGCCTGGAA
AGCCGCTCCAAGGTAAGCCTTCACGATACTCCCAAGTACGGGCTGATCTA
CCACGCGAGCCTGATTGGCCAGGCTAACCCAAAGCACAAGGGTAAGATCT
CGCGTGTGTTGGCGGCGAAGTGCGCCCTGGCGATCCGTGTGGACGCTCTC
GCCGACGAGACGGACACCACCATCGGCCTGGAAAGCCGCTCCAAGGTAAG
CCTT
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