mRNA_H-elongata_contig169758.4329.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig169758.4329.1
Unique NamemRNA_H-elongata_contig169758.4329.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig169758.4329.1 vs. uniprot
Match: D8LGJ4_ECTSI (Phospholipid-transporting ATPase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LGJ4_ECTSI)

HSP 1 Score: 217 bits (552), Expect = 5.060e-63
Identity = 108/122 (88.52%), Postives = 116/122 (95.08%), Query Frame = 1 
Query:    1 HVLGDPEAERMLLTLGSMCKSVVACRVSPAQKRLIVRLVKRGVVPTPVTLAIGDGANDVGMIQEAQIGVGISGKEGRQAVNNADFAIAQFRFLKRLMLVHGHWDYRRLCKVLGSGYWVYCVL 366
            HVLG+PEAERMLLTLGSMCKSV+ACRVSPAQKRLIVRLVKRGVVPTPVTL+IGDGANDVGMIQEAQIGVGISGKEGRQAVNN+DFAIAQFRFLKRLMLVHGHWDYRR+CKV+   ++   VL
Sbjct:  856 HVLGNPEAERMLLTLGSMCKSVIACRVSPAQKRLIVRLVKRGVVPTPVTLSIGDGANDVGMIQEAQIGVGISGKEGRQAVNNSDFAIAQFRFLKRLMLVHGHWDYRRVCKVILYSFYKNFVL 977
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BLAST of mRNA_H-elongata_contig169758.4329.1 vs. uniprot
Match: A0A836CHZ9_9STRA (Phospholipid-transporting ATPase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CHZ9_9STRA)

HSP 1 Score: 185 bits (469), Expect = 7.710e-52
Identity = 87/112 (77.68%), Postives = 101/112 (90.18%), Query Frame = 1 
Query:    1 HVLGDPEAERMLLTLGSMCKSVVACRVSPAQKRLIVRLVKRGVVPTPVTLAIGDGANDVGMIQEAQIGVGISGKEGRQAVNNADFAIAQFRFLKRLMLVHGHWDYRRLCKVL 336
            H+LG  + + + L +  MC+SVVACRVSPAQKRL+VRLVK+ V P P+TLAIGDGANDVGMIQEAQ+GVG+SGKEGRQAVNNADFAIAQFRFL+RL+LVHGHWDYRRLCKV+
Sbjct:  953 HILGHDDKQALFLRVACMCRSVVACRVSPAQKRLLVRLVKKNVRPAPITLAIGDGANDVGMIQEAQVGVGMSGKEGRQAVNNADFAIAQFRFLRRLLLVHGHWDYRRLCKVI 1064
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BLAST of mRNA_H-elongata_contig169758.4329.1 vs. uniprot
Match: W7TGI1_9STRA (Phospholipid-transporting ATPase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TGI1_9STRA)

HSP 1 Score: 180 bits (456), Expect = 4.310e-50
Identity = 89/122 (72.95%), Postives = 105/122 (86.07%), Query Frame = 1 
Query:    1 HVLGDPEAERMLLTLGSMCKSVVACRVSPAQKRLIVRLVKRGVVPTPVTLAIGDGANDVGMIQEAQIGVGISGKEGRQAVNNADFAIAQFRFLKRLMLVHGHWDYRRLCKVLGSGYWVYCVL 366
            HVLGDPE E M L L S+C+SVVACRVSPAQKRL+VRLVK GV P P+TL+IGDGANDV MIQEAQIGVGISGKEG+QAVN+ADFAIAQFR+L+RL+L+HG +DYRR+ KV+   ++   VL
Sbjct:  833 HVLGDPEYEAMFLRLSSICRSVVACRVSPAQKRLVVRLVKAGVKPMPITLSIGDGANDVAMIQEAQIGVGISGKEGQQAVNSADFAIAQFRYLRRLLLIHGRYDYRRMSKVILYSFYKNMVL 954
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BLAST of mRNA_H-elongata_contig169758.4329.1 vs. uniprot
Match: A0A7S4D8B9_HETAK (Phospholipid-transporting ATPase (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4D8B9_HETAK)

HSP 1 Score: 172 bits (437), Expect = 2.000e-48
Identity = 83/122 (68.03%), Postives = 99/122 (81.15%), Query Frame = 1 
Query:    1 HVLGDPEAERMLLTLGSMCKSVVACRVSPAQKRLIVRLVKRGVVPTPVTLAIGDGANDVGMIQEAQIGVGISGKEGRQAVNNADFAIAQFRFLKRLMLVHGHWDYRRLCKVLGSGYWVYCVL 366
            H++G  E ER  L +  +C+SV+ACRVSP QKRL+VRLVK GV   P+TLA+GDGANDVGMIQEAQ+GVGISGKEGRQAVN ADFAIAQFRFL+RL+ +HG WDYRR CKV+   ++   VL
Sbjct:  173 HIMGRAELERKFLRVARLCRSVIACRVSPEQKRLLVRLVKLGVEGAPITLAVGDGANDVGMIQEAQVGVGISGKEGRQAVNTADFAIAQFRFLRRLLFLHGRWDYRRTCKVVLYSFYKNMVL 294
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BLAST of mRNA_H-elongata_contig169758.4329.1 vs. uniprot
Match: A0A7S3XT36_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XT36_HETAK)

HSP 1 Score: 160 bits (406), Expect = 2.890e-48
Identity = 77/109 (70.64%), Postives = 92/109 (84.40%), Query Frame = 1 
Query:    1 HVLGDPEAERMLLTLGSMCKSVVACRVSPAQKRLIVRLVKRGVVPTPVTLAIGDGANDVGMIQEAQIGVGISGKEGRQAVNNADFAIAQFRFLKRLMLVHGHWDYRRLC 327
            HVLGD   E ML  + S+C +V+ACRVSP QK L+V +VK+ + PTPVTLAIGDGANDV MIQEAQ+GVGISG EG+QAVN++DFAIAQFR+LK L+LVHG W+YRRLC
Sbjct:   10 HVLGDDALEWMLFQVASLCNTVIACRVSPKQKALLVGMVKKYISPTPVTLAIGDGANDVNMIQEAQVGVGISGNEGQQAVNSSDFAIAQFRYLKPLLLVHGRWNYRRLC 118
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BLAST of mRNA_H-elongata_contig169758.4329.1 vs. uniprot
Match: A0A7S1ZM93_TRICV (Hypothetical protein (Fragment) n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1ZM93_TRICV)

HSP 1 Score: 164 bits (414), Expect = 2.710e-45
Identity = 79/122 (64.75%), Postives = 98/122 (80.33%), Query Frame = 1 
Query:    1 HVLGDPEAERMLLTLGSMCKSVVACRVSPAQKRLIVRLVKRGVVPTPVTLAIGDGANDVGMIQEAQIGVGISGKEGRQAVNNADFAIAQFRFLKRLMLVHGHWDYRRLCKVLGSGYWVYCVL 366
            H LGDP+ E ML  + S C SV+ACRVSP QK L+VR+V++ V PTPVTLAIGDGANDVGMIQEA +GVGISG EG+QAVN +DFAIAQFRFL+ L+L+HG W++ R+CKV+   ++   VL
Sbjct:  233 HFLGDPQYEEMLFAVASSCDSVIACRVSPKQKALMVRMVRKYVEPTPVTLAIGDGANDVGMIQEAHVGVGISGLEGQQAVNASDFAIAQFRFLRELLLIHGRWNFIRMCKVVLFSFYKNAVL 354
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BLAST of mRNA_H-elongata_contig169758.4329.1 vs. uniprot
Match: A0A7S2CMU9_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2CMU9_9STRA)

HSP 1 Score: 162 bits (409), Expect = 3.470e-45
Identity = 81/122 (66.39%), Postives = 98/122 (80.33%), Query Frame = 1 
Query:    1 HVLGDPEAERMLLTLGSMCKSVVACRVSPAQKRLIVRLVKRGVVPTPVTLAIGDGANDVGMIQEAQIGVGISGKEGRQAVNNADFAIAQFRFLKRLMLVHGHWDYRRLCKVLGSGYWVYCVL 366
             ++ D EA   LL LG  CK+V+ACRVSPAQK+LIV++VK G+   P+TL+IGDGANDV MIQEAQIGVGISGKEGRQAVN++DFAIAQFRFLKRL+LVHG W+Y R  KV+   ++   VL
Sbjct:   48 EIMDDEEARHQLLQLGCFCKAVLACRVSPAQKQLIVKMVKDGLPTKPITLSIGDGANDVPMIQEAQIGVGISGKEGRQAVNSSDFAIAQFRFLKRLLLVHGRWNYLRTAKVIIYSFYKNTVL 169
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BLAST of mRNA_H-elongata_contig169758.4329.1 vs. uniprot
Match: A0A7R9ZF58_9STRA (Hypothetical protein n=1 Tax=Pseudictyota dubia TaxID=2749911 RepID=A0A7R9ZF58_9STRA)

HSP 1 Score: 164 bits (414), Expect = 2.000e-44
Identity = 78/111 (70.27%), Postives = 94/111 (84.68%), Query Frame = 1 
Query:    1 HVLGDPEAERMLLTLGSMCKSVVACRVSPAQKRLIVRLVKRGVVPTPVTLAIGDGANDVGMIQEAQIGVGISGKEGRQAVNNADFAIAQFRFLKRLMLVHGHWDYRRLCKV 333
            H+LGDP+ E ML  + S C SV+ACRVSP QK L+VRLV++ V+PTPVTLAIGDGANDVGMIQEA +G+GISG EG+QAVN +DFAIAQFRFL+ L+L+HG W+Y RL KV
Sbjct: 1419 HLLGDPKLEEMLFAVASACDSVIACRVSPKQKALLVRLVRKYVLPTPVTLAIGDGANDVGMIQEAHVGIGISGLEGQQAVNASDFAIAQFRFLRDLLLIHGRWNYSRLAKV 1529
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BLAST of mRNA_H-elongata_contig169758.4329.1 vs. uniprot
Match: W7TQX5_9STRA (Phospholipid-transporting ATPase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TQX5_9STRA)

HSP 1 Score: 162 bits (411), Expect = 5.040e-44
Identity = 81/123 (65.85%), Postives = 100/123 (81.30%), Query Frame = 1 
Query:    1 HVLGDPEAERMLLTLGSMCKSVVACRVSPAQKRLIVRLVKRGVV-PTPVTLAIGDGANDVGMIQEAQIGVGISGKEGRQAVNNADFAIAQFRFLKRLMLVHGHWDYRRLCKVLGSGYWVYCVL 366
             + GD E ER+LL +  +CKSVVACRVSPAQKR+++RLVK+GV  P P+TLAIGDGANDV MIQEAQ+GVGISG+EGRQAVN+ADFAI+QFRF++ L+L HG W YRR  KV+   ++   VL
Sbjct: 1043 RIFGDWEMERLLLRVARLCKSVVACRVSPAQKRMLIRLVKKGVKHPKPITLAIGDGANDVAMIQEAQVGVGISGREGRQAVNSADFAISQFRFIEPLLLKHGRWSYRRTSKVILYSFYKNIVL 1165
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BLAST of mRNA_H-elongata_contig169758.4329.1 vs. uniprot
Match: A0A7S1C2W0_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1C2W0_9STRA)

HSP 1 Score: 152 bits (385), Expect = 5.670e-44
Identity = 76/117 (64.96%), Postives = 93/117 (79.49%), Query Frame = 1 
Query:    1 HVLGDPEAERMLLTLGSMCKSVVACRVSPAQKRLIVRLVK-----RGVVPTPVTLAIGDGANDVGMIQEAQIGVGISGKEGRQAVNNADFAIAQFRFLKRLMLVHGHWDYRRLCKVL 336
            H+LGDPE+E  L+ +  +CKSV+ACRVSP QK  +V LV+     R     P+ LAIGDGANDVGMIQ+AQ+GVGISGKEG QAVN++DFAIAQFRFL+RL+LVHG W YRR+ KV+
Sbjct:   46 HILGDPESEHALMVVAQLCKSVIACRVSPKQKADLVDLVRHRSPGRSGKNKPMCLAIGDGANDVGMIQQAQVGVGISGKEGLQAVNSSDFAIAQFRFLRRLLLVHGRWSYRRMSKVV 162
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig169758.4329.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LGJ4_ECTSI5.060e-6388.52Phospholipid-transporting ATPase n=1 Tax=Ectocarpu... [more]
A0A836CHZ9_9STRA7.710e-5277.68Phospholipid-transporting ATPase n=1 Tax=Tribonema... [more]
W7TGI1_9STRA4.310e-5072.95Phospholipid-transporting ATPase n=2 Tax=Monodopsi... [more]
A0A7S4D8B9_HETAK2.000e-4868.03Phospholipid-transporting ATPase (Fragment) n=1 Ta... [more]
A0A7S3XT36_HETAK2.890e-4870.64Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A7S1ZM93_TRICV2.710e-4564.75Hypothetical protein (Fragment) n=1 Tax=Trieres ch... [more]
A0A7S2CMU9_9STRA3.470e-4566.39Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A7R9ZF58_9STRA2.000e-4470.27Hypothetical protein n=1 Tax=Pseudictyota dubia Ta... [more]
W7TQX5_9STRA5.040e-4465.85Phospholipid-transporting ATPase n=2 Tax=Monodopsi... [more]
A0A7S1C2W0_9STRA5.670e-4464.96Hypothetical protein (Fragment) n=1 Tax=Bicosoecid... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig169758contigH-elongata_contig169758:454..1243 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score193.7
Seed ortholog evalue4.5e-47
Seed eggNOG ortholog2880.D8LGJ4
Preferred nameDNF3
KEGG rclassRC00002
KEGG koko:K01509,ko:K01530,ko:K14802
KEGG TC3.A.3.8
KEGG ReactionR00086
KEGG Pathwayko00230,ko04742,map00230,map04742
GOsGO:0000749,GO:0003674,GO:0003824,GO:0004012,GO:0005215,GO:0005319,GO:0005548,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005794,GO:0005798,GO:0005802,GO:0005886,GO:0005937,GO:0006810,GO:0006811,GO:0006820,GO:0006869,GO:0006886,GO:0006892,GO:0006900,GO:0006996,GO:0008104,GO:0008150,GO:0009987,GO:0010033,GO:0010876,GO:0012505,GO:0015031,GO:0015711,GO:0015748,GO:0015833,GO:0015914,GO:0016020,GO:0016021,GO:0016043,GO:0016050,GO:0016192,GO:0016197,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017111,GO:0019236,GO:0030133,GO:0030135,GO:0030136,GO:0030140,GO:0030427,GO:0031136,GO:0031137,GO:0031139,GO:0031224,GO:0031410,GO:0031982,GO:0031984,GO:0032456,GO:0033036,GO:0034204,GO:0034613,GO:0042221,GO:0042623,GO:0042886,GO:0042995,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043332,GO:0043492,GO:0043900,GO:0043902,GO:0044422,GO:0044424,GO:0044425,GO:0044431,GO:0044444,GO:0044446,GO:0044459,GO:0044463,GO:0044464,GO:0045184,GO:0045332,GO:0046907,GO:0046999,GO:0048193,GO:0048194,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0050896,GO:0051179,GO:0051234,GO:0051286,GO:0051641,GO:0051649,GO:0051716,GO:0061024,GO:0065007,GO:0065008,GO:0070727,GO:0070867,GO:0070887,GO:0071310,GO:0071444,GO:0071702,GO:0071705,GO:0071840,GO:0071944,GO:0097035,GO:0097708,GO:0098791,GO:0120025,GO:0120038,GO:2000241,GO:2000243
EggNOG free text desc.phospholipid-translocating ATPase activity
EggNOG OGsCOG0474@1,KOG0206@2759
EC3.6.1.3,3.6.3.1
COG Functional cat.P
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03009
Hectar predicted targeting categorymitochondrion
Exons2
Model size366
Cds size336
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig169758.4329.1prot_H-elongata_contig169758.4329.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig169758 454..1213 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929990.5247324-CDS-H-elongata_contig169758:453..6211622929990.5247324-CDS-H-elongata_contig169758:453..621Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig169758 454..621 -
1691679237.0956295-CDS-H-elongata_contig169758:453..6211691679237.0956295-CDS-H-elongata_contig169758:453..621Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig169758 454..621 -
1622929990.5713227-CDS-H-elongata_contig169758:1045..12131622929990.5713227-CDS-H-elongata_contig169758:1045..1213Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig169758 1046..1213 -
1691679237.11534-CDS-H-elongata_contig169758:1045..12131691679237.11534-CDS-H-elongata_contig169758:1045..1213Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig169758 1046..1213 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929990.5881894-UTR-H-elongata_contig169758:1213..12431622929990.5881894-UTR-H-elongata_contig169758:1213..1243Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig169758 1214..1243 -
1691679237.1271496-UTR-H-elongata_contig169758:1213..12431691679237.1271496-UTR-H-elongata_contig169758:1213..1243Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig169758 1214..1243 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig169758.4329.1

>prot_H-elongata_contig169758.4329.1 ID=prot_H-elongata_contig169758.4329.1|Name=mRNA_H-elongata_contig169758.4329.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=112bp
MLLTLGSMCKSVVACRVSPAQKRLIVRLVKRGVVPTPVTLAIGDGANDVG
MIQEAQIGVGISGKEGRQAVNNADFAIAQFRFLKRLMLVHGHWDYRRLCK
VLGSGYWVYCVL
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mRNA from alignment at H-elongata_contig169758:454..1243-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig169758.4329.1 ID=mRNA_H-elongata_contig169758.4329.1|Name=mRNA_H-elongata_contig169758.4329.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=790bp|location=Sequence derived from alignment at H-elongata_contig169758:454..1243- (Himanthalia elongata Himel1 dioecious)
CATGTTCTCGGCGACCCCGAAGCCGAAAGGATGCTCCTGACCCTGGGGAG
TATGTGCAAGAGCGTCGTGGCCTGCAGGGTCAGCCCCGCCCAGAAGCGTC
TTATTGTCCGGCTGGTGAAGAGGGGGGTCGTGCCAACGCCGGTCACCCTC
GCCATCGGAGATGGCGCAAACGACGTGGGAATGATCCAAGAGGCGCAGGT
ATCGCGGAAGTTACCCGAAAAGGCAGGGCAGGGAGGGGAGGGAGAAGGGG
GTATGACCGCATGGGAGAAAATAAGAGATATTTAATTTTGAAAAGGTTCA
TGATAAGATATGTTTTGTAGAGAAAGTAGGGGGTTTTCGTAGGAAACAAA
GGAGGTTTCAGTTTGTAGGAAAGAATAGGAAGAGCTGTACTAGCGAGCAG
GGGGAGAGCGATGGGCGGGAGCAATTTGGCCGCCGTGTTTAGTAGCCCCA
AGCGTCATTGCTCGGGGCAAGAAGCGTTTCACTCTGTGTCGTCAACGGTG
TTTTTCTTTTTGAGTTTGTGGCTGTGTGTGTGTGCTTTTCTCGGCAATAT
CCTGACAAATGGTCGACTTCACCCCTTTGCCCCCCCCATCCGTGCCTACA
CATCATGGAAACCTTTCTTCAGATTGGCGTCGGTATCAGCGGTAAGGAAG
GGCGTCAAGCGGTAAACAACGCCGATTTCGCGATCGCCCAGTTCCGGTTC
CTGAAGAGGCTGATGCTCGTACACGGTCACTGGGATTACCGACGGCTCTG
CAAGGTACTGGGTAGTGGGTACTGGGTATACTGTGTGCTT
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Coding sequence (CDS) from alignment at H-elongata_contig169758:454..1243-

>mRNA_H-elongata_contig169758.4329.1 ID=mRNA_H-elongata_contig169758.4329.1|Name=mRNA_H-elongata_contig169758.4329.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=672bp|location=Sequence derived from alignment at H-elongata_contig169758:454..1243- (Himanthalia elongata Himel1 dioecious)
ATGCTCCTGACCCTGGGGAGTATGTGCAAGAGCGTCGTGGCCTGCAGGGT
CAGCCCCGCCCAGAAGCGTCTTATTGTCCGGCTGGTGAAGAGGGGGGTCG
TGCCAACGCCGGTCACCCTCGCCATCGGAGATGGCGCAAACGACGTGGGA
ATGATCCAAGAGGCGCAGATGCTCCTGACCCTGGGGAGTATGTGCAAGAG
CGTCGTGGCCTGCAGGGTCAGCCCCGCCCAGAAGCGTCTTATTGTCCGGC
TGGTGAAGAGGGGGGTCGTGCCAACGCCGGTCACCCTCGCCATCGGAGAT
GGCGCAAACGACGTGGGAATGATCCAAGAGGCGCAGATTGGCGTCGGTAT
CAGCGGTAAGGAAGGGCGTCAAGCGGTAAACAACGCCGATTTCGCGATCG
CCCAGTTCCGGTTCCTGAAGAGGCTGATGCTCGTACACGGTCACTGGGAT
TACCGACGGCTCTGCAAGGTACTGGGTAGTGGGTACTGGGTATACTGTGT
GCTTATTGGCGTCGGTATCAGCGGTAAGGAAGGGCGTCAAGCGGTAAACA
ACGCCGATTTCGCGATCGCCCAGTTCCGGTTCCTGAAGAGGCTGATGCTC
GTACACGGTCACTGGGATTACCGACGGCTCTGCAAGGTACTGGGTAGTGG
GTACTGGGTATACTGTGTGCTT
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