mRNA_H-elongata_contig153709.3544.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig153709.3544.1
Unique NamemRNA_H-elongata_contig153709.3544.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig153709.3544.1 vs. uniprot
Match: D7G5X0_ECTSI (Putative homoaconitate hydratase, N-terminal part n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5X0_ECTSI)

HSP 1 Score: 121 bits (303), Expect = 2.980e-32
Identity = 59/61 (96.72%), Postives = 61/61 (100.00%), Query Frame = 1 
Query:   16 KVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFSLEGEIPDHLLAKV 198
            +VLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQF+LEGEIPDHLLAKV
Sbjct:  210 EVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFALEGEIPDHLLAKV 270
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BLAST of mRNA_H-elongata_contig153709.3544.1 vs. uniprot
Match: A0A6H5KHV3_9PHAE (3-isopropylmalate dehydratase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KHV3_9PHAE)

HSP 1 Score: 119 bits (299), Expect = 4.080e-30
Identity = 58/60 (96.67%), Postives = 60/60 (100.00%), Query Frame = 1 
Query:   16 KVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFSLEGEIPDHLLAK 195
            +VLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQF+LEGEIPDHLLAK
Sbjct:  200 EVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFALEGEIPDHLLAK 259
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BLAST of mRNA_H-elongata_contig153709.3544.1 vs. uniprot
Match: X2KY86_SCYLO (3-isopropylmalate dehydratase n=1 Tax=Scytosiphon lomentaria TaxID=27967 RepID=X2KY86_SCYLO)

HSP 1 Score: 118 bits (296), Expect = 1.210e-29
Identity = 57/60 (95.00%), Postives = 60/60 (100.00%), Query Frame = 1 
Query:   16 KVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFSLEGEIPDHLLAK 195
            +VLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQF+LEGE+PDHLLAK
Sbjct:  219 EVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFALEGEMPDHLLAK 278
[Close]
BLAST of mRNA_H-elongata_contig153709.3544.1 vs. uniprot
Match: A0A835YVF1_9STRA (3-isopropylmalate dehydratase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YVF1_9STRA)

HSP 1 Score: 113 bits (283), Expect = 4.840e-28
Identity = 54/65 (83.08%), Postives = 61/65 (93.85%), Query Frame = 1 
Query:    1 YCLSVKVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFSLEGEIPDHLLAK 195
            +C   +VLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIK+PA+M+F+L GEIPD+LLAK
Sbjct:  143 HCRPGEVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKTPATMRFALNGEIPDYLLAK 207
[Close]
BLAST of mRNA_H-elongata_contig153709.3544.1 vs. uniprot
Match: A0A6M0CDJ2_9CYAN (3-isopropylmalate dehydratase (Fragment) n=1 Tax=Okeania sp. SIO2H7 TaxID=2607802 RepID=A0A6M0CDJ2_9CYAN)

HSP 1 Score: 106 bits (264), Expect = 1.220e-26
Identity = 49/60 (81.67%), Postives = 57/60 (95.00%), Query Frame = 1 
Query:   16 KVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFSLEGEIPDHLLAK 195
            +VLFGTDSHTCNAGAFGQFATGIGNTDA+F+MGTGKLL+K PA+M+F L GE+PD+LLAK
Sbjct:  129 EVLFGTDSHTCNAGAFGQFATGIGNTDAAFIMGTGKLLVKVPATMRFVLNGEMPDYLLAK 188
[Close]
BLAST of mRNA_H-elongata_contig153709.3544.1 vs. uniprot
Match: W7TZ20_9STRA (3-isopropylmalate dehydratase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TZ20_9STRA)

HSP 1 Score: 108 bits (269), Expect = 5.840e-26
Identity = 51/60 (85.00%), Postives = 57/60 (95.00%), Query Frame = 1 
Query:   16 KVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFSLEGEIPDHLLAK 195
            +VLFGTDSHTCNAGAFGQFATGIGNTDA+FV+GTGKLL+K PASM+F L GEIPD+LLAK
Sbjct:  182 EVLFGTDSHTCNAGAFGQFATGIGNTDAAFVLGTGKLLLKVPASMKFELNGEIPDYLLAK 241
[Close]
BLAST of mRNA_H-elongata_contig153709.3544.1 vs. uniprot
Match: K9SF49_9CYAN (Homoaconitate hydratase family protein n=1 Tax=Pseudanabaena sp. PCC 7367 TaxID=82654 RepID=K9SF49_9CYAN)

HSP 1 Score: 107 bits (267), Expect = 6.660e-26
Identity = 50/60 (83.33%), Postives = 57/60 (95.00%), Query Frame = 1 
Query:   16 KVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFSLEGEIPDHLLAK 195
            +VLFGTDSHTCNAGAFGQFATGIGNTDA+FVMGTGKLL+K PA+M+F+ EGE+P HLLAK
Sbjct:  129 EVLFGTDSHTCNAGAFGQFATGIGNTDAAFVMGTGKLLLKVPATMRFTFEGEMPPHLLAK 188
[Close]
BLAST of mRNA_H-elongata_contig153709.3544.1 vs. uniprot
Match: A0A383VB52_TETOB (Aconitase domain-containing protein (Fragment) n=1 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383VB52_TETOB)

HSP 1 Score: 103 bits (258), Expect = 6.990e-26
Identity = 47/65 (72.31%), Postives = 58/65 (89.23%), Query Frame = 1 
Query:    1 YCLSVKVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFSLEGEIPDHLLAK 195
            +C   +VLFGTDSHTCNAGAFGQFATG+GNTDA F++GTGKLLIK P +M+F L+GE+P++LLAK
Sbjct:  172 HCKPGEVLFGTDSHTCNAGAFGQFATGVGNTDAGFILGTGKLLIKVPPTMRFVLDGEMPEYLLAK 236
[Close]
BLAST of mRNA_H-elongata_contig153709.3544.1 vs. uniprot
Match: A0A6P1AAH6_9CYAN (3-isopropylmalate dehydratase large subunit n=2 Tax=Oscillatoriales TaxID=1150 RepID=A0A6P1AAH6_9CYAN)

HSP 1 Score: 107 bits (266), Expect = 9.160e-26
Identity = 50/60 (83.33%), Postives = 57/60 (95.00%), Query Frame = 1 
Query:   16 KVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFSLEGEIPDHLLAK 195
            +VLFGTDSHTCNAGAFGQFATGIGNTDA F+MGTGKLLIK PA+M+F L+GE+PD+LLAK
Sbjct:  129 EVLFGTDSHTCNAGAFGQFATGIGNTDAGFIMGTGKLLIKVPATMRFVLDGEMPDYLLAK 188
[Close]
BLAST of mRNA_H-elongata_contig153709.3544.1 vs. uniprot
Match: A0A2V0NV93_9CHLO (3-isopropylmalate dehydratase n=1 Tax=Raphidocelis subcapitata TaxID=307507 RepID=A0A2V0NV93_9CHLO)

HSP 1 Score: 106 bits (265), Expect = 1.730e-25
Identity = 49/65 (75.38%), Postives = 58/65 (89.23%), Query Frame = 1 
Query:    1 YCLSVKVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASMQFSLEGEIPDHLLAK 195
            +C   +VLFGTDSHTCNAGAFGQFATG+GNTDA F++GTGKLLIK P +M+F LEGE+PD+LLAK
Sbjct:  162 HCKPGEVLFGTDSHTCNAGAFGQFATGVGNTDAGFILGTGKLLIKVPPTMRFLLEGEMPDYLLAK 226
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig153709.3544.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G5X0_ECTSI2.980e-3296.72Putative homoaconitate hydratase, N-terminal part ... [more]
A0A6H5KHV3_9PHAE4.080e-3096.673-isopropylmalate dehydratase n=1 Tax=Ectocarpus s... [more]
X2KY86_SCYLO1.210e-2995.003-isopropylmalate dehydratase n=1 Tax=Scytosiphon ... [more]
A0A835YVF1_9STRA4.840e-2883.083-isopropylmalate dehydratase n=1 Tax=Tribonema mi... [more]
A0A6M0CDJ2_9CYAN1.220e-2681.673-isopropylmalate dehydratase (Fragment) n=1 Tax=O... [more]
W7TZ20_9STRA5.840e-2685.003-isopropylmalate dehydratase n=2 Tax=Monodopsidac... [more]
K9SF49_9CYAN6.660e-2683.33Homoaconitate hydratase family protein n=1 Tax=Pse... [more]
A0A383VB52_TETOB6.990e-2672.31Aconitase domain-containing protein (Fragment) n=1... [more]
A0A6P1AAH6_9CYAN9.160e-2683.333-isopropylmalate dehydratase large subunit n=2 Ta... [more]
A0A2V0NV93_9CHLO1.730e-2575.383-isopropylmalate dehydratase n=1 Tax=Raphidocelis... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig153709contigH-elongata_contig153709:287..496 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score121.7
Seed ortholog evalue1.4e-25
Seed eggNOG ortholog2880.D7G5X0
Preferred nameLEU1
KEGG rclassRC00497,RC00976,RC00977,RC01041,RC01046,RC03072
KEGG koko:K00749,ko:K01702,ko:K01703
KEGG ReactionR03896,R03898,R03968,R04001,R08620,R08624,R08628,R08634,R08641,R08645,R10170
KEGG Pathwayko00290,ko00660,ko00966,ko01100,ko01110,ko01210,ko01230,map00290,map00660,map00966,map01100,map01110,map01210,map01230
KEGG ModuleM00432,M00535
GOsGO:0003674,GO:0003824,GO:0003861,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006082,GO:0006520,GO:0006551,GO:0006790,GO:0006807,GO:0008150,GO:0008152,GO:0008652,GO:0009058,GO:0009081,GO:0009082,GO:0009098,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0009987,GO:0010035,GO:0010038,GO:0010494,GO:0016053,GO:0016143,GO:0016144,GO:0016829,GO:0016835,GO:0016836,GO:0016853,GO:0016866,GO:0019748,GO:0019752,GO:0019757,GO:0019758,GO:0019760,GO:0019761,GO:0032991,GO:0035770,GO:0036464,GO:0042221,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043436,GO:0044237,GO:0044238,GO:0044249,GO:0044272,GO:0044281,GO:0044283,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0044550,GO:0046394,GO:0046686,GO:0050486,GO:0050896,GO:0071704,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901607,GO:1901657,GO:1901659,GO:1990904
EggNOG free text desc.3-isopropylmalate dehydratase activity
EggNOG OGsCOG0065@1,KOG0454@2759
EC2.4.1.183,4.2.1.33,4.2.1.35
COG Functional cat.E
CAZyGT5
BiGG ReactioniMM904.YGL009C,iND750.YGL009C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01601,ko00000,ko00001,ko00002,ko01000,ko01003
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionHomoaconitate hydratase
Ec32 orthologEc-13_001950.1
Exons1
Model size210
Cds size210
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929908.8732076-CDS-H-elongata_contig153709:286..4961622929908.8732076-CDS-H-elongata_contig153709:286..496Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig153709 287..496 -
1691679204.2450652-CDS-H-elongata_contig153709:286..4961691679204.2450652-CDS-H-elongata_contig153709:286..496Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig153709 287..496 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig153709.3544.1prot_H-elongata_contig153709.3544.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig153709 287..496 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig153709.3544.1

>prot_H-elongata_contig153709.3544.1 ID=prot_H-elongata_contig153709.3544.1|Name=mRNA_H-elongata_contig153709.3544.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=70bp
YCLSVKVLFGTDSHTCNAGAFGQFATGIGNTDASFVMGTGKLLIKSPASM
QFSLEGEIPDHLLAKVDLL*
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mRNA from alignment at H-elongata_contig153709:287..496-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig153709.3544.1 ID=mRNA_H-elongata_contig153709.3544.1|Name=mRNA_H-elongata_contig153709.3544.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=210bp|location=Sequence derived from alignment at H-elongata_contig153709:287..496- (Himanthalia elongata Himel1 dioecious)
TACTGTCTCTCGGTCAAGGTTTTGTTCGGGACAGACTCTCACACGTGCAA
CGCGGGCGCCTTCGGACAGTTCGCCACCGGGATAGGGAACACCGATGCCT
CGTTCGTCATGGGCACCGGGAAGCTCTTGATCAAGAGCCCCGCGTCCATG
CAGTTCTCCCTCGAAGGGGAGATTCCGGACCATCTCCTGGCAAAGGTAGA
CCTCCTCTAG
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Coding sequence (CDS) from alignment at H-elongata_contig153709:287..496-

>mRNA_H-elongata_contig153709.3544.1 ID=mRNA_H-elongata_contig153709.3544.1|Name=mRNA_H-elongata_contig153709.3544.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=420bp|location=Sequence derived from alignment at H-elongata_contig153709:287..496- (Himanthalia elongata Himel1 dioecious)
TACTGTCTCTCGGTCAAGGTTTTGTTCGGGACAGACTCTCACACGTGCAA
CGCGGGCGCCTTCGGACAGTTCGCCACCGGGATAGGGAACACCGATGCCT
CGTTCGTCATGGGCACCGGGAAGCTCTTGATCAAGAGCCCCGCGTCCATG
CAGTTCTCCCTCGAAGGGGAGATTCCGGACCATCTCCTGGCAAAGGTAGA
CCTCCTCTAGTACTGTCTCTCGGTCAAGGTTTTGTTCGGGACAGACTCTC
ACACGTGCAACGCGGGCGCCTTCGGACAGTTCGCCACCGGGATAGGGAAC
ACCGATGCCTCGTTCGTCATGGGCACCGGGAAGCTCTTGATCAAGAGCCC
CGCGTCCATGCAGTTCTCCCTCGAAGGGGAGATTCCGGACCATCTCCTGG
CAAAGGTAGACCTCCTCTAG
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