mRNA_H-elongata_contig88767.16389.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig88767.16389.1
Unique NamemRNA_H-elongata_contig88767.16389.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig88767.16389.1 vs. uniprot
Match: A0A6H5JE16_9PHAE (MHD domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JE16_9PHAE)

HSP 1 Score: 198 bits (504), Expect = 5.130e-60
Identity = 99/103 (96.12%), Postives = 102/103 (99.03%), Query Frame = 1 
Query:   16 SNHIMEREEMEQKPVFTVEGVTYVYVQYNNLILMSVSKRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 324
            SNHI EREEMEQKP+FTVEGVT+VYVQYNNLILMSV+KRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ
Sbjct:   34 SNHIQEREEMEQKPIFTVEGVTFVYVQYNNLILMSVTKRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 136
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BLAST of mRNA_H-elongata_contig88767.16389.1 vs. uniprot
Match: A0A835Z8U5_9STRA (Clathrin assembly complex, medium subunit n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z8U5_9STRA)

HSP 1 Score: 178 bits (452), Expect = 2.810e-52
Identity = 88/103 (85.44%), Postives = 97/103 (94.17%), Query Frame = 1 
Query:   16 SNHIMEREEMEQKPVFTVEGVTYVYVQYNNLILMSVSKRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 324
            S +I EREE +Q+PVFT EG T+VY++YNNL+LMSV+KRNSNVALMLVYLYKL DVF+DYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ
Sbjct:   34 SLYIQEREETDQQPVFTEEGYTFVYIRYNNLLLMSVTKRNSNVALMLVYLYKLTDVFRDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 136
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BLAST of mRNA_H-elongata_contig88767.16389.1 vs. uniprot
Match: A0A7S3M290_9STRA (Hypothetical protein (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3M290_9STRA)

HSP 1 Score: 160 bits (404), Expect = 9.110e-48
Identity = 74/103 (71.84%), Postives = 91/103 (88.35%), Query Frame = 1 
Query:   16 SNHIMEREEMEQKPVFTVEGVTYVYVQYNNLILMSVSKRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 324
            S HI E++EM+ +PVFT +G+TY+Y++ NNL+LM+ +K+NSNV L+L YLYKL  VFKDYFGELEEESIRDNFV++YELLDETMDFGYPQT ESKILRE +TQ
Sbjct:   34 SMHIQEKDEMDLRPVFTEDGITYIYIKCNNLLLMAATKKNSNVTLILFYLYKLTTVFKDYFGELEEESIRDNFVVVYELLDETMDFGYPQTCESKILREVVTQ 136
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BLAST of mRNA_H-elongata_contig88767.16389.1 vs. uniprot
Match: W7U403_9STRA (Ap-1 complex subunit mu-1-like isoform 1 n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U403_9STRA)

HSP 1 Score: 166 bits (419), Expect = 2.740e-47
Identity = 79/103 (76.70%), Postives = 94/103 (91.26%), Query Frame = 1 
Query:   16 SNHIMEREEMEQKPVFTVEGVTYVYVQYNNLILMSVSKRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 324
            + ++ E+EE EQ+P+FT EG T+ Y++YNNL L+SV+KRNSNVALMLVYL +LVDVFK+YFGELEEESIRDNFVIIYEL+DETMDFGYPQT E+KILREYITQ
Sbjct:   34 ATYLQEKEESEQRPIFTEEGCTFAYLKYNNLFLLSVTKRNSNVALMLVYLSRLVDVFKEYFGELEEESIRDNFVIIYELMDETMDFGYPQTTEAKILREYITQ 136
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BLAST of mRNA_H-elongata_contig88767.16389.1 vs. uniprot
Match: A0A7S3HYH8_9SPIT (Hypothetical protein (Fragment) n=1 Tax=Favella ehrenbergii TaxID=182087 RepID=A0A7S3HYH8_9SPIT)

HSP 1 Score: 152 bits (384), Expect = 6.290e-45
Identity = 71/108 (65.74%), Postives = 89/108 (82.41%), Query Frame = 1 
Query:    1 CSPFVSNHIMEREEMEQKPVFTVEGVTYVYVQYNNLILMSVSKRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 324
            C    S H++E EE +++PV+   G TYVY++YNNL +M+V++RNSN A++L++LY+LVDVFKDYF ELEEESIRDNFVI YEL+DE MDFGYPQ  E KILREYITQ
Sbjct:   14 CVERFSGHVLEAEEADERPVWLEHGTTYVYIKYNNLYIMAVTQRNSNAAMILLFLYRLVDVFKDYFKELEEESIRDNFVITYELMDEMMDFGYPQVSEPKILREYITQ 121
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BLAST of mRNA_H-elongata_contig88767.16389.1 vs. uniprot
Match: A0A7S2Y0S1_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2Y0S1_9STRA)

HSP 1 Score: 159 bits (402), Expect = 7.500e-45
Identity = 71/103 (68.93%), Postives = 94/103 (91.26%), Query Frame = 1 
Query:   16 SNHIMEREEMEQKPVFTVEGVTYVYVQYNNLILMSVSKRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 324
            + +I   EEME +P+FT +G+T++Y++YNNL L++V+K+NSNV L+L+YLYKLV+VFKDYFGELEEESIRDNFVIIYELLDET+DFGYPQ ++SK+L+E+ITQ
Sbjct:   34 AQYIQNTEEMEARPIFTEDGITFIYIRYNNLYLLTVTKQNSNVGLILIYLYKLVEVFKDYFGELEEESIRDNFVIIYELLDETVDFGYPQALDSKVLKEFITQ 136
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BLAST of mRNA_H-elongata_contig88767.16389.1 vs. uniprot
Match: F0YHG2_AURAN (MHD domain-containing protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YHG2_AURAN)

HSP 1 Score: 158 bits (400), Expect = 1.450e-44
Identity = 73/101 (72.28%), Postives = 91/101 (90.10%), Query Frame = 1 
Query:   22 HIMEREEMEQKPVFTVEGVTYVYVQYNNLILMSVSKRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 324
            ++ E +EM+Q+P+FT EG T+ Y ++NNL LM V+KRNSN+AL+L+YLY+LV VFKDYFGEL+EESIRDNFVIIYEL+DETMDFGYPQ M+SKILRE+ITQ
Sbjct:   36 YLQENDEMDQRPIFTDEGFTFAYTKHNNLFLMCVTKRNSNIALLLMYLYRLVTVFKDYFGELDEESIRDNFVIIYELMDETMDFGYPQAMDSKILREFITQ 136
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BLAST of mRNA_H-elongata_contig88767.16389.1 vs. uniprot
Match: A0A662XXV4_9STRA (Clat_adaptor_s domain-containing protein (Fragment) n=1 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662XXV4_9STRA)

HSP 1 Score: 150 bits (378), Expect = 2.110e-44
Identity = 71/103 (68.93%), Postives = 92/103 (89.32%), Query Frame = 1 
Query:   16 SNHIMEREEMEQKPVFTVEGVTYVYVQYNNLILMSVSKRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 324
            ++++ E+E+ EQ+PVFT +G T+VY+++NNL LM+V+K NSNVALML+YL ++  VF+DYFGELEEESIRDNFVII+ELLDETMD GYPQT E++ILREYITQ
Sbjct:   34 THYVQEKEDSEQRPVFTEDGYTFVYLKHNNLYLMTVTKVNSNVALMLMYLNRICQVFQDYFGELEEESIRDNFVIIFELLDETMDHGYPQTTEARILREYITQ 136
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BLAST of mRNA_H-elongata_contig88767.16389.1 vs. uniprot
Match: A0A7S2R9Z0_9STRA (Hypothetical protein n=3 Tax=Dictyochophyceae TaxID=39119 RepID=A0A7S2R9Z0_9STRA)

HSP 1 Score: 157 bits (398), Expect = 3.750e-44
Identity = 74/103 (71.84%), Postives = 91/103 (88.35%), Query Frame = 1 
Query:   16 SNHIMEREEMEQKPVFTVEGVTYVYVQYNNLILMSVSKRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 324
            + +I E EE + +P+FT +G T+VY+++NNL LM+V+KRN+NVALM  YLY+LV VFKDYFGEL+EESIRDNFVIIYELLDET DFGYPQ+M+SKILREYITQ
Sbjct:   48 ARYIQETEEFDMRPIFTEDGYTFVYIKHNNLYLMTVTKRNANVALMFTYLYRLVSVFKDYFGELDEESIRDNFVIIYELLDETNDFGYPQSMDSKILREYITQ 150
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BLAST of mRNA_H-elongata_contig88767.16389.1 vs. uniprot
Match: A0A0M5K9V4_EMIHU (Adaptor protein complex 1 subunit mu (Fragment) n=1 Tax=Emiliania huxleyi TaxID=2903 RepID=A0A0M5K9V4_EMIHU)

HSP 1 Score: 145 bits (366), Expect = 1.370e-42
Identity = 67/108 (62.04%), Postives = 87/108 (80.56%), Query Frame = 1 
Query:    1 CSPFVSNHIMEREEMEQKPVFTVEGVTYVYVQYNNLILMSVSKRNSNVALMLVYLYKLVDVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQ 324
            C    S H++E E  +++PV+   G TY+Y++YNNL +M+V++RNSN A++L++LY+LV+V KDYF ELEEESIRDNFVI YEL+DE MDFGYPQ  E KILREYITQ
Sbjct:   29 CVERFSGHVLEAEANDERPVWLEHGTTYIYIKYNNLYIMAVTQRNSNAAMILLFLYRLVEVLKDYFKELEEESIRDNFVITYELMDEMMDFGYPQVSEPKILREYITQ 136
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig88767.16389.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JE16_9PHAE5.130e-6096.12MHD domain-containing protein n=2 Tax=Ectocarpus T... [more]
A0A835Z8U5_9STRA2.810e-5285.44Clathrin assembly complex, medium subunit n=1 Tax=... [more]
A0A7S3M290_9STRA9.110e-4871.84Hypothetical protein (Fragment) n=1 Tax=Spumella e... [more]
W7U403_9STRA2.740e-4776.70Ap-1 complex subunit mu-1-like isoform 1 n=2 Tax=M... [more]
A0A7S3HYH8_9SPIT6.290e-4565.74Hypothetical protein (Fragment) n=1 Tax=Favella eh... [more]
A0A7S2Y0S1_9STRA7.500e-4568.93Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
F0YHG2_AURAN1.450e-4472.28MHD domain-containing protein n=1 Tax=Aureococcus ... [more]
A0A662XXV4_9STRA2.110e-4468.93Clat_adaptor_s domain-containing protein (Fragment... [more]
A0A7S2R9Z0_9STRA3.750e-4471.84Hypothetical protein n=3 Tax=Dictyochophyceae TaxI... [more]
A0A0M5K9V4_EMIHU1.370e-4262.04Adaptor protein complex 1 subunit mu (Fragment) n=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig88767contigH-elongata_contig88767:1980..2633 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score189.1
Seed ortholog evalue1e-45
Seed eggNOG ortholog2880.D7FLA6
KEGG koko:K12393
KEGG Pathwayko04142,map04142
GOsGO:0000139,GO:0000323,GO:0001775,GO:0002090,GO:0002092,GO:0002119,GO:0002164,GO:0002252,GO:0002263,GO:0002274,GO:0002275,GO:0002283,GO:0002366,GO:0002376,GO:0002443,GO:0002444,GO:0002446,GO:0002478,GO:0002495,GO:0002504,GO:0003674,GO:0005048,GO:0005102,GO:0005215,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005765,GO:0005773,GO:0005774,GO:0005794,GO:0005798,GO:0005802,GO:0005829,GO:0005886,GO:0005905,GO:0006810,GO:0006886,GO:0006887,GO:0006950,GO:0006955,GO:0006970,GO:0006971,GO:0006996,GO:0007032,GO:0007034,GO:0007041,GO:0007275,GO:0008104,GO:0008150,GO:0008565,GO:0009605,GO:0009628,GO:0009790,GO:0009791,GO:0009792,GO:0009893,GO:0009987,GO:0010256,GO:0010604,GO:0012505,GO:0012506,GO:0012510,GO:0015031,GO:0015833,GO:0016020,GO:0016032,GO:0016043,GO:0016050,GO:0016192,GO:0019222,GO:0019882,GO:0019884,GO:0019886,GO:0021700,GO:0022607,GO:0030030,GO:0030031,GO:0030100,GO:0030117,GO:0030118,GO:0030119,GO:0030120,GO:0030121,GO:0030122,GO:0030125,GO:0030128,GO:0030130,GO:0030131,GO:0030132,GO:0030133,GO:0030135,GO:0030136,GO:0030139,GO:0030140,GO:0030141,GO:0030154,GO:0030658,GO:0030659,GO:0030660,GO:0030662,GO:0030665,GO:0030666,GO:0030667,GO:0030669,GO:0031090,GO:0031323,GO:0031325,GO:0031410,GO:0031982,GO:0031984,GO:0032438,GO:0032501,GO:0032502,GO:0032588,GO:0032879,GO:0032940,GO:0032991,GO:0033036,GO:0033059,GO:0033218,GO:0033365,GO:0034613,GO:0035579,GO:0035646,GO:0036230,GO:0040025,GO:0042119,GO:0042277,GO:0042581,GO:0042886,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043299,GO:0043312,GO:0043473,GO:0043476,GO:0043482,GO:0043485,GO:0043933,GO:0044085,GO:0044403,GO:0044419,GO:0044422,GO:0044424,GO:0044425,GO:0044431,GO:0044433,GO:0044437,GO:0044444,GO:0044446,GO:0044459,GO:0044464,GO:0044782,GO:0045055,GO:0045176,GO:0045184,GO:0045321,GO:0045334,GO:0045807,GO:0046903,GO:0046907,GO:0048002,GO:0048066,GO:0048259,GO:0048260,GO:0048475,GO:0048513,GO:0048518,GO:0048522,GO:0048569,GO:0048731,GO:0048753,GO:0048757,GO:0048856,GO:0048869,GO:0050690,GO:0050750,GO:0050789,GO:0050794,GO:0050896,GO:0050931,GO:0051049,GO:0051050,GO:0051128,GO:0051130,GO:0051179,GO:0051234,GO:0051641,GO:0051649,GO:0051704,GO:0051716,GO:0060255,GO:0060271,GO:0060341,GO:0060627,GO:0061512,GO:0065003,GO:0065007,GO:0070325,GO:0070727,GO:0070925,GO:0071702,GO:0071705,GO:0071840,GO:0071944,GO:0072657,GO:0097499,GO:0097500,GO:0097708,GO:0098588,GO:0098693,GO:0098791,GO:0098796,GO:0098797,GO:0098805,GO:0098852,GO:0099503,GO:0120031,GO:0120036,GO:1900242,GO:1900244,GO:1903421,GO:1903423,GO:1903441,GO:1990778
EggNOG free text desc.vesicle-mediated transport
EggNOG OGsKOG0937@1,KOG0937@2759
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04131
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionClathrin assembly complex, medium subunit
Ec32 orthologEc-15_004940.1
Exons2
Model size342
Cds size315
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig88767.16389.1prot_H-elongata_contig88767.16389.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig88767 2007..2633 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931288.6230636-UTR-H-elongata_contig88767:1979..20061622931288.6230636-UTR-H-elongata_contig88767:1979..2006Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig88767 1980..2006 +
1691679774.9075694-UTR-H-elongata_contig88767:1979..20061691679774.9075694-UTR-H-elongata_contig88767:1979..2006Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig88767 1980..2006 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931288.6370947-CDS-H-elongata_contig88767:2006..21531622931288.6370947-CDS-H-elongata_contig88767:2006..2153Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig88767 2007..2153 +
1691679774.9236023-CDS-H-elongata_contig88767:2006..21531691679774.9236023-CDS-H-elongata_contig88767:2006..2153Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig88767 2007..2153 +
1622931288.664983-CDS-H-elongata_contig88767:2465..26331622931288.664983-CDS-H-elongata_contig88767:2465..2633Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig88767 2466..2633 +
1691679774.9403095-CDS-H-elongata_contig88767:2465..26331691679774.9403095-CDS-H-elongata_contig88767:2465..2633Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig88767 2466..2633 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig88767.16389.1

>prot_H-elongata_contig88767.16389.1 ID=prot_H-elongata_contig88767.16389.1|Name=mRNA_H-elongata_contig88767.16389.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=105bp
MEREEMEQKPVFTVEGVTYVYVQYNNLILMSVSKRNSNVALMLVYLYKLV
DVFKDYFGELEEESIRDNFVIIYELLDETMDFGYPQTMESKILREYITQV
RTDY*
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mRNA from alignment at H-elongata_contig88767:1980..2633+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig88767.16389.1 ID=mRNA_H-elongata_contig88767.16389.1|Name=mRNA_H-elongata_contig88767.16389.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=654bp|location=Sequence derived from alignment at H-elongata_contig88767:1980..2633+ (Himanthalia elongata Himel1 dioecious)
TGTTCACCGTTCGTCAGTAATCACATCATGGAACGTGAGGAGATGGAACA
GAAGCCCGTGTTCACCGTGGAAGGCGTTACTTACGTCTACGTACAATACA
ACAACCTCATCCTCATGAGCGTTTCCAAGCGAAACAGTAACGTGGCTCTC
ATGCTGGTGTACCTCTATAAGCTGGTGAGCCGAGTCGCGACATGCCCCAG
CATTCGATAGGCGATTTAATTTGGGACGCGTGACGTGTGACGCGTTCCTG
TCTAGTAGCCCTGCATTGGGTTAGCTAGGGTTAGCTAAGTCGGGAGTTGT
TAGGTTAGGGTTAGTTAGGGTTAGGATTAGGGTACACGGCAAACAAGTAG
TGGAACGGTTAGAGTTAGCCAGCGTATTGGATGCTTGTTGCTTGTCGATG
TGTCCGTTTTGATACTGATACATGGTATTTTTAACGGTACGGTATGGTAC
GGTACAACACGCGGTACGATACGGTACGATACCCAGGTTGACGTGTTTAA
GGACTACTTCGGGGAGCTGGAGGAGGAATCAATCAGGGATAATTTCGTCA
TCATCTACGAACTGCTGGACGAAACTATGGACTTCGGGTACCCCCAGACG
ATGGAGTCCAAAATACTGAGGGAATATATCACGCAGGTGAGGACTGATTA
CTGA
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Coding sequence (CDS) from alignment at H-elongata_contig88767:1980..2633+

>mRNA_H-elongata_contig88767.16389.1 ID=mRNA_H-elongata_contig88767.16389.1|Name=mRNA_H-elongata_contig88767.16389.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=630bp|location=Sequence derived from alignment at H-elongata_contig88767:1980..2633+ (Himanthalia elongata Himel1 dioecious)
ATGGAACGTGAGGAGATGGAACAGAAGCCCGTGTTCACCGTGGAAGGCGT
TACTTACGTCTACGTACAATACAACAACCTCATCCTCATGAGCGTTTCCA
AGCGAAACAGTAACGTGGCTCTCATGCTGGTGTACCTCTATAAGCTGATG
GAACGTGAGGAGATGGAACAGAAGCCCGTGTTCACCGTGGAAGGCGTTAC
TTACGTCTACGTACAATACAACAACCTCATCCTCATGAGCGTTTCCAAGC
GAAACAGTAACGTGGCTCTCATGCTGGTGTACCTCTATAAGCTGGTTGAC
GTGTTTAAGGACTACTTCGGGGAGCTGGAGGAGGAATCAATCAGGGATAA
TTTCGTCATCATCTACGAACTGCTGGACGAAACTATGGACTTCGGGTACC
CCCAGACGATGGAGTCCAAAATACTGAGGGAATATATCACGCAGGTGAGG
ACTGATTACTGAGTTGACGTGTTTAAGGACTACTTCGGGGAGCTGGAGGA
GGAATCAATCAGGGATAATTTCGTCATCATCTACGAACTGCTGGACGAAA
CTATGGACTTCGGGTACCCCCAGACGATGGAGTCCAAAATACTGAGGGAA
TATATCACGCAGGTGAGGACTGATTACTGA
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