mRNA_H-elongata_contig12140.1638.1 (mRNA) Himanthalia elongata Himel1 dioecious

BLAST of mRNA_H-elongata_contig12140.1638.1 vs. uniprot
Match: D7FLN1_ECTSI (Asparagine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FLN1_ECTSI)

HSP 1 Score: 816 bits (2107), Expect = 3.310e-288
Identity = 453/660 (68.64%), Postives = 503/660 (76.21%), Query Frame = 1

Query:    1 LAGIQLQPKFGLSQGEIEAACPAARGVVLRVSRPSPASTV----MVAQSNAILRALAEAAPESLLRGRTEFESAQVDQWLETCWMEFEVPLKALSAASTNQSVIAEAKRNLISFLEVANARLSTSTLLVGQRTTIADVAVACAAQDAIESSGAG-VIIFGPKDLSGLPHLLRWYQTVIRHPAFASPSCPPPDDVRGTPASAALDQNNIGGAKPQKKDVAAAPASTSRGFLSNANGDAGYGDAAEKPLPVIGEGGDLLSSAAVVQGARTLALAPARFRRKRVRVRELLASGTVLVGQKAVVKGWLRTARAASKGKLLFLVVDDGSCSETV--VVSREAPGFDAVANCGGVGASVSVLGEVVASKGKGQEVEIKAEEVEVLGCVYEPDGTVGASTYPLAKKAHSLEFLRTIAHLRPRTKASKRETGRTAMHLTPNSIMHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLLA-----EGEKIGSSNGEVVPVTSTGAVDYSKAGLFGLLDFFARRTCLTVSGQLNVETHACALGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDDIDLAEDYLKYLVAYALAHCQGDLEYFESCPGGEPGLRKRLQNVLENDFVRMTYTELVELVRKDAKEGKVRIGI 1944
            +AGI L  K G+S+ E+EA  P A+G+VL+V RP  A+ V     V QSNAILR LA A  +S L GR+EFESAQ DQWLETCW E EVP +AL+AAS NQ+VIAEAKR+L+SFL  ANA LSTST LVG+RTTIADV +ACAA+D IE S AG     G +DL+GLPH+LRWY+TV  HPAF++ +                                                      A ++PLPV+GE GDL+SSA+VVQG+R L +APARFRRKRVRVRELLASGT +VGQKAVVKGWLRTARAASKG LLFLVVDDGSCS TV  VVS+ APGFDA A+CGGVGASVSVLG VVASKGKGQ+VEIKA+EVEVLGCVYEPDGTVGASTYPLAKKAHSLEFLR+IAHLRPRTK                  MHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLL      EG    ++ G   P+T  GAVDYSK       DFFARRTCLTVSGQLNVETHAC+LGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDD+DLAEDYLKYLVAYAL HCQGDLE+FE+CPGGEPGLR+RL+NVL+NDFVRMTYTELV+LVRKDAKEGKV   +
Sbjct:   41 MAGIDLDLKTGMSEEEMEAFAPLAKGLVLKVCRPGQAAPVGGEEAVTQSNAILRTLAMAKADSSLYGRSEFESAQADQWLETCWKELEVPAQALAAASGNQAVIAEAKRHLLSFLATANATLSTSTFLVGERTTIADVGLACAARDLIEGSVAGGAASLGARDLAGLPHVLRWYRTVTHHPAFSATAVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-------XXXXXXXXXXXXXXXAGDEPLPVVGESGDLMSSASVVQGSRRLEVAPARFRRKRVRVRELLASGTSVVGQKAVVKGWLRTARAASKGALLFLVVDDGSCSGTVQVVVSKGAPGFDAAASCGGVGASVSVLGTVVASKGKGQDVEIKADEVEVLGCVYEPDGTVGASTYPLAKKAHSLEFLRSIAHLRPRTK------------------MHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLLPDAGGEEGNGHAAAAGGGPPMTPAGAVDYSK-------DFFARRTCLTVSGQLNVETHACSLGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDDMDLAEDYLKYLVAYALGHCQGDLEFFETCPGGEPGLRERLKNVLQNDFVRMTYTELVDLVRKDAKEGKVEFSV 668          

BLAST of mRNA_H-elongata_contig12140.1638.1 vs. uniprot
Match: A0A835YW11_9STRA (Asparagine--tRNA ligase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YW11_9STRA)

HSP 1 Score: 558 bits (1437), Expect = 1.760e-187
Identity = 324/637 (50.86%), Postives = 410/637 (64.36%), Query Frame = 1

Query:   64 PAARGVVLRVSRPSPASTVMVAQSNAILRALAEAAPESLLRGRTEFESAQVDQWLETCWMEFEVPLKALSAASTNQSVIAEAKRNLISFLEVANARLSTSTLLVGQRTTIADVAVACAAQDAIESSGAGVIIFGPKDLSGLPHLLRWYQTVIRHPAFASPSCPPPDDVRGTPASAALDQNNIGGAKPQKKDVAAAPASTSRGFLSNANGDAGYGDAA----EKPLPVIGEGGDLLSSAAVVQGARTLALAPARFRRKRVRVRELLASGTVLVGQKAVVKGWLRTARAASKGKLLFLVVDDGSCSET--VVVSREAP-GFDAVANCGGVGASVSVLGEVVASKGKGQEVEIKAEEVEVLGCVYEPDGTVGASTYPLAKKAHSLEFLRTIAHLRPRTKASKRETGRTAMHLTPNSIMHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLL---AEGEKIGSSNGEV-VPVTSTGAVDYSKAGLFGLLDFFARRTCLTVSGQLNVETHACALGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDDIDLAEDYLKYLVAYALAHCQGDLEYFES--CPGGEPGLRKRLQNVLENDFVRMTYTELVELVRKDAKEGKVR 1935
            PAA+ + LR++RP+ A    + QSNAI+R L +A+  S L G TEF  +QVD WLE CW+E E PL  L  A  +  VIAEAK+ ++  L   NA L+  T LVG R T AD+A+ACAAQ  +   GA   +   KDL+ LPH  RWY TV  H AFA+          GT                                 +N+  DA +  A+    +KPL ++                     APA++RR+RVRV+ELLA G    G+  VV+GWL+TAR+ +K  LLFLVV+DGSC  +  VV SR+   GF A A+CGGVGA V+V G ++ S  KGQ +E++A++VEVLG VY+ DG++GA+ YPLAKKAHSLE+LRT AHLRPRT                  +++SA MRVR+AMA+ATH+FFN+RGF+Y+HTP++TGADCEGAGEQF V+TLL   AEG   G   G V +P  + G++DY+K       DFF++R CLTVSGQLNVET+  A+ DVYTFGPTFRAENSHTSRHLAEFWMIEPE+AFADL+DD++LAEDYLKYLVA+AL HC  DL YFES  CPGGE  LR RL+NVLENDFVR+TYT  V L+ +    GKV+
Sbjct:   60 PAAKALALRITRPTSAHPSYITQSNAIVRTLIDASETSGLYGDTEFARSQVDSWLELCWLELETPLAVLQQAKGHGDVIAEAKKLILHALTAVNAALAARTYLVGNRLTAADIALACAAQPIL---GAAASVLSGKDLATLPHAARWYMTVTHHAAFAT----------GTSXXXXXXXXXXXXXXXXXXXX--------XXXXANSTVDAAFYQASAVHTDKPLSMV---------------------APAKYRRRRVRVKELLARGAAAAGETVVVRGWLKTARSGAKDTLLFLVVNDGSCHSSLQVVASRDGTEGFAAAASCGGVGACVAVEGPIIESPAKGQVIEMQAKKVEVLGPVYQSDGSIGAADYPLAKKAHSLEYLRTHAHLRPRT------------------MVYSAVMRVRNAMAYATHRFFNERGFVYVHTPLLTGADCEGAGEQFVVTTLLPAAAEGHACG---GRVELPRLADGSIDYTK-------DFFSKRACLTVSGQLNVETYCSAMSDVYTFGPTFRAENSHTSRHLAEFWMIEPEIAFADLEDDMNLAEDYLKYLVAHALEHCDEDLTYFESKDCPGGEADLRARLRNVLENDFVRITYTRAVALLEEHIASGKVK 626          

BLAST of mRNA_H-elongata_contig12140.1638.1 vs. uniprot
Match: W7TB32_9STRA (Asparagine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TB32_9STRA)

HSP 1 Score: 450 bits (1157), Expect = 2.260e-145
Identity = 296/673 (43.98%), Postives = 378/673 (56.17%), Query Frame = 1

Query:    1 LAGIQLQPKFGLSQGEIEAACPAARGVVLRVSRPSPASTVMVAQSNAILRALAEAAPESLLRGRTEFESAQVDQWLETCWMEFEVPLKALSAASTNQSVIA---EAKRNLISFLEVANARLSTSTLLVGQRTTIADVAVACAAQDAIESSGAGVIIFGPKDLSGLPHLLRWYQTVIRHPAF--ASPS-----------CPPPDDVRGTPASAALDQNNIGGAKPQKKDVAAAPASTSRGFLSNANGDAGYGDAAEKP--LPVIGEGGDLLSSAAVVQGARTLALAPARFRRKRVRVRELLAS-GTVLVGQKAVVKGWLRTARAASKGKLLFLVVDDGSCS---ETVVVSREAPGFDAV--ANCGGVGASVSVLGEVVASKGKGQEVEIKAEEVEVLGCVYE-PDGTVGASTYPLAKKAHSLEFLRTIAHLRPRTKASKRETGRTAMHLTPNSIMHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLLAEGEKIGSSNGEVVPVTSTGAVDYSKAGLFGLLDFFARRTCLTVSGQLNVETHACALGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDDIDLAEDYLKYLVAYALAHCQGDLEYFESCPGGEPGLRKRLQNVLENDFVRMTYTELVELVRKDAKEGKVRIGI 1944
            LAG++L          + +  P  + +VL V   S  S  ++A+SN  LR LAE +P + L G T +E+  VDQWL+  +   E P   +      Q  +A    A+R +   + V +  L  ST L G + T+ADV V CA  D +    AG +      L   P +LRW+ T++  P F   SP             P     R   A   L +N  G  +P K  +A A A+ +    S   G AG G ++ KP  LP  G    L                   F R+R  +++LL   G   VG+   +KGW+RT RA +KG LLF+ ++DGS +   + V+ + +  GF     A+CGGVGAS S+ G +V S  +GQ VE+ A  VEVLG VY   DG VG   YPL+KKAHS+E+LR  AHLR RT    R TG              + MRVRHAMA+ATHKFFNDRGF Y+HTP++TGADCEGAGE F V+TLL E           +P+   G++DYS+       DFF RRT LTVSGQLNVET+AC L DVYTFGPTFRAENSHT+RHLAEFWMIEPE+AFADL+ D+ LAEDYLKY V YAL H + DL   E  PGGE GLR+RL+NVLEN FVR+TYTE V  + KD    KV   +
Sbjct:   26 LAGVELSVSLQEDPEILSSLVPHGKSLVLEVQSAS-GSPFLIARSNVALRFLAENSPAAGLYGETLYEAGLVDQWLDFSFNSLETPAAVMHLPPDAQVPLAVKETARREVEGAVRVLDEALEKSTFLAGHKVTLADVGVFCALYDLVGVENAGSMSIA---LENFPGVLRWFLTLLHQPCFQHVSPGEADKMKRLQAKLPSVGSTRRKLAEGPLAEN--GNRRPSKAGIAGARATQASAAKS---GPAGGGRSSTKPPLLPKDGPDSSL-------------------FDRRRTVIKQLLVERGEAAVGETVTIKGWVRTIRAGNKGALLFIELNDGSTALSMQAVLEADKTQGFAEATPASCGGVGASFSITGTLVRSPAEGQAVELHATVVEVLGKVYGGEDGGVGGKLYPLSKKAHSMEYLRCNAHLRART----RVTG--------------SVMRVRHAMAYATHKFFNDRGFKYVHTPLITGADCEGAGEMFTVTTLLPE------KGAPRLPIRPDGSIDYSQ-------DFFGRRTSLTVSGQLNVETYACGLSDVYTFGPTFRAENSHTTRHLAEFWMIEPEIAFADLEMDMALAEDYLKYCVWYALRHNEEDLVLLEKVPGGEAGLRERLRNVLENPFVRITYTEAVATLEKDLAAEKVAFQV 639          

BLAST of mRNA_H-elongata_contig12140.1638.1 vs. uniprot
Match: A0A8J2SLQ1_9STRA (Asparagine--tRNA ligase n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SLQ1_9STRA)

HSP 1 Score: 413 bits (1062), Expect = 1.560e-131
Identity = 280/650 (43.08%), Postives = 356/650 (54.77%), Query Frame = 1

Query:    1 LAGIQLQPKFGLSQGEIEAACPAARGVVLRVSRPSPASTVMVAQSNAILRALAEAAPESLLRGRTEFESAQVDQWLETCWMEFEVPLKALSAASTNQSVIAEAKRNLISFLEVANARLSTSTLLVGQRTTIADVAVACAAQDAIESSGAGVIIFGPKDLSGLPHLLRWYQTVIRHPAFASPSCPPPDDVRGTPASAALDQNNIGGAKPQKKDVAAAPASTSRGFLSNANGDAGYGDAAEKPLPVIGEGGDLLSSAAVVQGARTLALAPARFRRKRVRVRELLASGTVLVGQKAVVKGWLRTARAASKGKLLFLVVDDGSCSETVVV---SREAPGFDAVANCGGVGASVSVLGEVVASKGKGQEVEIKAEEVEVLGCVYE-PDGTVGASTYPLAKKAHSLEFLRTIAHLRPRTKASKRETGRTAMHLTPNSIMHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLLA-EGEKIGSSNGEVVPVTSTGAVDYSKAGLFGLLDFFARRTCLTVSGQLNVETHACALGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDDIDLAEDYLKYLVAYALAHCQGDLEYFESCPGGEPGLRKRLQNVLENDFVRMTYTELVELVRKDAKEGKVR 1935
            LA I+L+      + E+   CP A  VVL ++R  P     V +S+A+L+ ++   P + L G   F   +VDQWLE    E EV + A +  + N+ V+  A +++       +A L  +T L G R + AD AV C  + A+++        GP        LLRWY T +  PAF+S        + G  A     +    G  P  K  AAA A  S                                +A  V   RTL   P  F R R R++ELL  G   VG   VV GW+RT R A KG  LF+ ++DGSC  ++ V   S    GF A   CGG GAS+ V G VV S GKGQ +EI A+ + VLGC    P  TVGA TYPLAKK H+LE LRT AHLRPR++                    SA +R+R+A+AFATH+FF  RGFLY+HTP++T ADCEGAGEQF V+TLL  E +K        +P T  G VDYSK       DFF R+  LTVSGQLNVETHA +L D YTFGPTFRAENS+TSRHLAEFWMIEPEV+FA L DDI LAEDYLKY VA AL  C  DLE+FE       GLR RL+ ++E  F R+ YTE + +++K+ K G+ +
Sbjct:   47 LANIKLRITAAKDEAELTKLCPHAATVVL-MTRNGP-----VTRSDAVLKYVSGLNPATELCGADGFGRGRVDQWLEFGTHELEVAVVAATTHAKNKEVVNRATKDVQQACRTLDAALQKTTYLAGDRVSAADCAVVCVLRAALKNQLIATSKLGPS-------LLRWYLTCLHAPAFSS--------ILGADAKNDALKALPAGKAPTSKKTAAAKAKAS-------------------------------PTAKAVADPRTL-FTPT-FARSRERLKELLGRGAAAVGATVVVAGWVRTLREAMKGATLFVELNDGSCLASIQVVCGSDSTEGFAAAKACGGAGASLRVEGAVVESPGKGQTIEILAKAITVLGCTRGGPGRTVGAQTYPLAKKYHTLEHLRTHAHLRPRSRVG------------------SAVVRLRNALAFATHEFFQQRGFLYVHTPLITAADCEGAGEQFTVTTLLPPEHDKA-----PKLP-TVDGRVDYSK-------DFFGRQASLTVSGQLNVETHAVSLADCYTFGPTFRAENSNTSRHLAEFWMIEPEVSFATLADDIALAEDYLKYCVAAALCRCADDLEFFEGSKN--EGLRDRLKAIVETPFKRLEYTEAISVLQKEMKAGRAK 609          

BLAST of mRNA_H-elongata_contig12140.1638.1 vs. uniprot
Match: A0A7S2ARR7_9STRA (Asparagine--tRNA ligase n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2ARR7_9STRA)

HSP 1 Score: 411 bits (1056), Expect = 2.160e-130
Identity = 276/659 (41.88%), Postives = 355/659 (53.87%), Query Frame = 1

Query:    1 LAGIQLQPKFGLSQGEIEAACPAARGVVLRVSRPSPASTVMVAQSNAILRALAEAAPESLLRGRTEFESAQVDQWLETCWMEFEVPLKALSA-----------ASTNQSVIAEAKRNLISFLEVANARLSTSTLLVGQRTTIADVAVACAAQDAIESSGAGVIIFGPKDLSGLPHLLRWYQTVIRHPAFASPSCPPPDDVRGTPASAALDQNNIGGAKPQKKDVAAAPASTSRGFLSNANGDAGYGDAAEKPLPVIGEGGDLLSSAAVVQGARTLALAPARFRRKRVRVRELLASGTVLVGQKAVVKGWLRTARAASKGKLLFLVVDDGSCSETV--VVSREAPGFDAVANCGGVGASVSVLGEVVASKGKGQEVEIKAEEVEVLGCVYEPD-GTVGASTYPLAKKAHSLEFLRTIAHLRPRTKASKRETGRTAMHLTPNSIMHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLLAEGEKIGSSNGEVVPVTSTGAVDYSKAGLFGLLDFFARRTCLTVSGQLNVETHACALGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDDIDLAEDYLKYLVAYALAHCQGDLEYFESCPGGEPGLRKRLQNVLENDFVRMTYTELVELVRKDAKEGKVR 1935
            L G+ L+    L + E E+  P AR  VL     S      + Q++ ILR LAE  P+  L G T FE A V+QWLE CW E EV + AL+            AS  Q +  +A R L    E  N  L++ T +VG   T+A+ A+     + +        +  P D    PH+LRW++T+    A  +     P D +            +   KP K  VAA                           P    GG  LS+  V          P +F+R R RV++LL  G   VG+   V GW +T RAA+KGK+LF+V+ DGSC++ +  V+    PGF   +NCGG GAS S+LG+VV S G GQ VE+  +  EVLG VY  D G VG   YPL+KK HS E LR  AHLRPR ++                  ++A  RVR+ MAFATHKFF +RGF Y+HTP++T +DCEGAGE FAV+T L    K  S  G++        VDYSK       DFF +   LTVSGQLNVETHAC L D YTFGPTFRAE+SHTSRHLAEFWMIEPE+ FADLKDD DLAEDYLK+ V  AL  C  +LE  E  P G+  LR+RLQ ++E  F R TYTE ++++  +  +G V+
Sbjct:   48 LVGVPLESLI-LKEDEFESLLPNARSCVLVTKDGS------LTQTSTILRYLAELRPDLTLSGATGFEQAMVNQWLEFCWFELEVLVGALTIRPDESEGTFDPASIRQILSRDAARAL----ECLNKHLASHTFMVGNGITLAEAALVPTLVEVLSK------MLMPGDARLYPHVLRWFKTLAHGNADFAAVLQVPTDFKP-----------LAPLKPSK--VAAXXXXXXXXXXXXXXXXXXXXX--XXXXPTASPGG--LSAGNVKDPF------PMKFKRTRTRVKDLLKRGAQAVGEIITVCGWAQTVRAANKGKILFVVITDGSCTQALQCVIDSNIPGFAEASNCGGTGASFSLLGKVVESPGAGQSVELVVQSAEVLGKVYGGDNGEVGGKYYPLSKKGHSAEHLRDHAHLRPRVRS------------------YAATHRVRNTMAFATHKFFFERGFQYVHTPLITASDCEGAGEMFAVTTQL---PKDHSKKGKI-------DVDYSK-------DFFGKPAGLTVSGQLNVETHACGLSDCYTFGPTFRAEDSHTSRHLAEFWMIEPEICFADLKDDADLAEDYLKFCVHSALTECGDELELLEKIPNGDSMLRERLQGIVEKPFARKTYTECIKILLAEVAKGAVK 631          

BLAST of mRNA_H-elongata_contig12140.1638.1 vs. uniprot
Match: A0A7S2R1X4_9STRA (Asparagine--tRNA ligase (Fragment) n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2R1X4_9STRA)

HSP 1 Score: 404 bits (1039), Expect = 4.210e-128
Identity = 267/684 (39.04%), Postives = 373/684 (54.53%), Query Frame = 1

Query:   10 IQLQPKFGLSQGEIEAACPAARGVVLRVSRPSPASTVMVAQSNAILRALAEAAPESLLRGRTEFESAQVDQWLETCWMEFEVPLKALSAASTNQSVIAEAKRNLISFLEVANARLSTSTLLVGQRTTIADVAVACAAQDAIESSGAGVIIFGPKDLSG--LPHLLRWYQTVIRHPAFASPSCPPPDDVRGTPASAALDQNNIGGAKPQKKDVAAAPASTSRGFLSNANGDAGYGDAAEKPLPVIGEGGDLLSSAAVVQGARTLALAPARFRRKRVRVRELLAS-GTVLVGQKAVVKGWLRTARAASKGKLLFLVVDDGSCSET---VVVSREAPGFDAVANCGGVGASVSVLGEVVASKGKGQEVEIKAEEVEVLGCVY--EPDGT-VGASTYPLAKKAHSLEFLRTIAHLRPRTKASKRETGRTAMHLTPNSIMHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLLA----------------EGEKIGSS--------------NGEVVPVTSTGAVDYSKAGLFGLLDFFARRTCLTVSGQLNVETHACALGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDDIDLAEDYLKYLVAYALAHCQGDLEYFESCPGGEPGLRKRLQNVLENDFVRMTYTELVELVRKDAKEGKVRIGI 1944
            + LQ +  ++  ++ A  P ++G+VL +       +     S A+++ALA  AP S + G++  ESA +D W++      E+PL+A S          + K ++   + V + +L+ ST +VG   TIAD+++ CA   A         ++ P  +    L  + +WY +V R   F S       D     A   +D ++  G  P                                  PV+ +                       ++R R+RV+E+LA+ G   +G+   V GW RT R A+KG LLF+ ++DGS  ++   ++ S +  G++     GG G+S  + G +VAS+G GQ VE+KA+  ++LG VY  + +GT VG   YP++KKAH+LE +RT AHLRPR +                  +H+AAMRVRHAMAFATHKFFND GFLYIHTPI+T ADCEGAGEQF V+TLL                 E +K+ +               + +++     GAVDYS        DFF +R  LTVSGQLNVETHACAL DVYTFGPTFRAENSHT+RHL+EFWMIEPE+AFA+L+DDI+LAEDYLKY V YAL  C  DLE+FE+ P GE GLR RL+NVL+N F R+TYTE +E++ K  KE  V+  +
Sbjct:   44 LSLQKEVVVTNEDLLALSPISKGLVLSILDGHIGGS-----SAAMMKALASLAPGSGIAGQSLLESANIDSWIQYGATSIEIPLEASSEV--------DVKDDIAKAIAVLSTKLADSTYMVGDSITIADISLCCALYKAASDK-----LWNPSSIDDEKLTIVGQWYGSVTRQDFFISALHALKSDGEVL-AGNTVDVSSFNGVAP----------------------------------PVVNK----------------------LYKRNRIRVKEVLANDGANFMGKTVTVAGWTRTTRNANKGLLLFIELNDGSTGQSLQCILESDKTEGYEESKLSGGAGSSFQITGTIVASQGDGQSVELKADTSKLLGAVYGGDAEGTKVGGMLYPMSKKAHTLEHMRTHAHLRPRAR------------------LHAAAMRVRHAMAFATHKFFNDNGFLYIHTPILTCADCEGAGEQFGVTTLLGADHNQPGVDLPLHEKPEEKKLSNKELKRLAKKAAKDAESNKLIEEKVPGAVDYSG-------DFFGKRANLTVSGQLNVETHACALSDVYTFGPTFRAENSHTTRHLSEFWMIEPEIAFANLEDDINLAEDYLKYCVQYALDMCADDLEFFENSPFGEAGLRDRLKNVLDNPFKRLTYTEAIEILIKAYKEDGVKFEV 627          

BLAST of mRNA_H-elongata_contig12140.1638.1 vs. uniprot
Match: A0A7S1DE08_CYCTE (Asparagine--tRNA ligase n=1 Tax=Cyclophora tenuis TaxID=216820 RepID=A0A7S1DE08_CYCTE)

HSP 1 Score: 400 bits (1029), Expect = 3.090e-127
Identity = 254/642 (39.56%), Postives = 361/642 (56.23%), Query Frame = 1

Query:  118 VMVAQSNAILRALAEAAPESLLRGRTEFESAQVDQWLETCWMEFEVPLKALSAASTNQSVIAEAKRNLISFLEVANARLSTSTLLVGQRTTIADVAVACAAQDAIESSGAGVIIFGPKDLSGLPHLLRWYQTVIRHPAFASPSCPPPDDVRGTPASAALDQNNIGGAKPQKKDVAAAPASTSRGFLSNANGDAGYGDAAEKPLPVIGEGGDLLSSAAVVQGARTLALAPARFRRKRVRVRELLAS-GTVLVGQKAVVKGWLRTARAASKGKLLFLVVDDGSCSETVVVSREAPGFDAVANC---GGVGASVSVLGEVVASKGKGQEVEIKAEEVEVLGCVY--EPDGT-VGASTYPLAKKAHSLEFLRTIAHLRPRTKASKRETGRTAMHLTPNSIMHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLLA-----------------EGEK---------------IGSSNGEVVPVTSTGAVDYSKAGLFGLLDFFARRTCLTVSGQLNVETHACALGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDDIDLAEDYLKYLVAYALAHCQGDLEYFESCPGGEPGLRKRLQNVLENDFVRMTYTELVELVRKDAKEG 1926
            V +  S+A++R +A  AP + + G ++ + A V+ W+   W   + P++AL   +   +      + +I+ L      L   T +VG   T+AD+++  + + A+++      ++ PK+ + L    R+YQT+I  P F +                         A+   K  +A   + S+G  S+++G     + A  P PV+ +                       +RR R+R++E+LA+ G   + Q   V GW RT R A+KG+LLF+ ++DGS   ++    +A   +  A C   GG G+S+ ++G++++S   GQ VE++    ++LG VY    +GT VG   YP++KKAH+LEF+R +AHLRPR        GR          +H+AAMRVRHAMA+ATH FF++ GFLYIHTPI+T ADCEGAGEQFAV+T+L                  E EK                G    +   V   GAVDY+        DFFARR  LTVSGQLNVETHACAL DVYTFGPTFRAENSHT RHL+EFWMIEPE+AFADL DDI+LAEDYLKY V +AL +C  DLE+FE+ P GE GLR RL+NVL+N F R+TYTE +E+++K  ++G
Sbjct:    3 VKIGGSDAMMRTIASLAPATKVAGGSDVDKALVEGWMSFVWASLDAPMEALQQINDQVTT-----KGVIAALGTLECHLHYQTYMVGHSITLADISLVVSLRSAVDAK-----LWEPKEGTNLE---RYYQTMIHQPFFQT-------------------------AEKCMKSTSAPVGAPSKGSSSSSSGSGVLLNGA--PTPVVNK----------------------MYRRHRIRIKEILANDGASYMDQTVTVAGWTRTTRNANKGQLLFVELNDGSVGTSLQCVLDATTTEGFAECKASGGTGSSLQLVGKLISSPAAGQSVELQVTSGKLLGAVYGGNHEGTEVGGMLYPMSKKAHTLEFMRDVAHLRPR--------GR----------LHAAAMRVRHAMAYATHNFFHNHGFLYIHTPIITCADCEGAGEQFAVTTMLGNEPTETGITLPLNEPPKEEEKKMSKKEMKRLKKKAGAGKDPNKPEEVKVVGAVDYTN-------DFFARRANLTVSGQLNVETHACALSDVYTFGPTFRAENSHTGRHLSEFWMIEPEIAFADLTDDINLAEDYLKYCVQFALDNCADDLEFFENNPHGEVGLRARLRNVLDNPFKRLTYTEAIEILQKAVEDG 557          

BLAST of mRNA_H-elongata_contig12140.1638.1 vs. uniprot
Match: A0A7S2WSY6_9STRA (Asparagine--tRNA ligase n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WSY6_9STRA)

HSP 1 Score: 384 bits (985), Expect = 5.380e-123
Identity = 211/386 (54.66%), Postives = 261/386 (67.62%), Query Frame = 1

Query:  799 APA-RFRRKRVRVRELLASGTVLVGQKAVVKGWLRTARAASKGKLLFLVVDDGSCSETV--VVSREAPGFDAVAN--CGGVGASVSVLGEVVASKGKGQEVEIKAEEVEVLGCVYEPDGT-VGASTYPLAK--KAHSLEFLRTIAHLRPRTKASKRETGRTAMHLTPNSIMHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLLAEGEKIGSSNGEVVPVTSTGAVDYSKAGLFGLLDFFARRTCLTVSGQLNVETHACALGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDDIDLAEDYLKYLVAYALAHCQGDLEYFESCPGGEPGLRKRLQNVLENDFVRMTYTELVELVRKDAKEGKV 1932
            APA RF+RKR+R+ EL+  GT LVG+  VV+GW +T R A KG +LF+ + DGS S  V  VV     GF+A     CGG  AS S+ G+VV S+G GQ +E++A +VEVLG V+  DG   G   YPLA+  K HS EFLR++AHLRPRT+A                   +A  RVR AMA+ATH+FFN+RGFLYIHTP++TGADCEGAGE F+V+TL+ + +       E +P T  GA+D+SK       DFF  +T LTVSGQLNVETH C+L DVYTFGPTFRAENSHTSRHLAEFWMIEPE+AFA L+DD+DLAEDYLK+ V YAL  C  DL   E  P  +  LR RL N+LE  F R+TYTE + ++ K+   GKV
Sbjct:   24 APAMRFKRKRIRIAELMQLGTTLVGKTVVVQGWAKTIREAGKGSILFIQLSDGSSSGDVQVVVDAGVEGFEAARPNVCGGTDASFSISGKVVESQGAGQSIELQAVKVEVLGQVFAGDGDQAGGKFYPLARNRKGHSFEFLRSVAHLRPRTRAF------------------AAVQRVRSAMAYATHQFFNERGFLYIHTPLITGADCEGAGEMFSVTTLIKDPK-------EPLPTTKDGAIDFSK-------DFFGAQTGLTVSGQLNVETHCCSLSDVYTFGPTFRAENSHTSRHLAEFWMIEPEIAFAQLEDDMDLAEDYLKHCVHYALTVCGEDLAALEKVPSWDSELRSRLMNILERPFTRLTYTEAISILEKEVASGKV 377          

BLAST of mRNA_H-elongata_contig12140.1638.1 vs. uniprot
Match: A0A1E7ERJ5_9STRA (Asparagine--tRNA ligase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7ERJ5_9STRA)

HSP 1 Score: 381 bits (978), Expect = 5.460e-122
Identity = 213/393 (54.20%), Postives = 271/393 (68.96%), Query Frame = 1

Query:  811 FRRKRVRVRELLAS-GTVLVGQKAVVKGWLRTARAASKGKLLFLVVDDGSCSET---VVVSREAPGFDAVANCGGVGASVSVLGEVVASKGKGQEVEIKAEEVEVLGCVY--EPDGT-VGASTYPLAKKAHSLEFLRTIAHLRPRTKASKRETGRTAMHLTPNSIMHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLLAEGEKIGSSNGEV---VPVTST--------GAVDYSKAGLFGLLDFFARRTCLTVSGQLNVETHACALGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDDIDLAEDYLKYLVAYALAHCQGDLEYFESCPGGEPGLRKRLQNVLENDFVRMTYTELVELVRKDAKEGKVR 1935
            +RR RVRV+E++A+ GT  + +   V GW RT R A+KG+LLF+ ++DGS  E+   V+ S +  GFD   N GG GAS  ++G ++AS+G GQ VE+     ++LG VY    +GT +G + YP++KKAH+LE +R  AHLR R        GR          +H+AAMR+RH MA+ATH FF++ GFLYIHTPI+TGADCEGAGEQF V+TLL      GS + E    +P+  T        GAVDYSK       DFF +R  LTVSGQLNVETHACAL DVYTFGPTFRAENSHT RHLAEFWMIEPE+ FADL DDI+LAEDYLKY V +AL +C  DLE+FE+ P GE GLR RL+NVL+N F R+TYTE +++++K  K+G V+
Sbjct:   14 YRRHRVRVKEVMANEGTQFLDKTITVAGWARTTRNANKGQLLFVEINDGSSGESIQCVLDSEKTEGFDECKNSGGTGASFQMVGTLIASQGDGQAVELAVTTGKLLGAVYGGNIEGTEIGGALYPMSKKAHTLEHMREHAHLRAR--------GR----------VHAAAMRIRHCMAYATHTFFHNHGFLYIHTPILTGADCEGAGEQFGVTTLL------GSDHLETDVTIPIDPTKPVNETVVGAVDYSK-------DFFGKRVNLTVSGQLNVETHACALSDVYTFGPTFRAENSHTGRHLAEFWMIEPEICFADLSDDINLAEDYLKYCVKFALENCAEDLEFFENSPHGEVGLRDRLKNVLDNPFQRLTYTEAIKVLQKAEKDGDVK 375          

BLAST of mRNA_H-elongata_contig12140.1638.1 vs. uniprot
Match: H3G8N3_PHYRM (Asparagine--tRNA ligase n=19 Tax=Peronosporaceae TaxID=4777 RepID=H3G8N3_PHYRM)

HSP 1 Score: 375 bits (962), Expect = 7.210e-120
Identity = 222/396 (56.06%), Postives = 263/396 (66.41%), Query Frame = 1

Query:  766 AVVQGARTLALAPARFRRKRVRVRELLASGTVLVGQKAVVKGWLRTARAASKGKLLFLVVDDGSCS---ETVVVSREAPGFDAVANCGGVGASVSVLGEVVASKGKGQEVEIKAEEVEVLGCVYEPDGTVGASTYPLAKKAHSLEFLRTIAHLRPRTKASKRETGRTAMHLTPNSIMHSAAMRVRHAMAFATHKFFNDRGFLYIHTPIVTGADCEGAGEQFAVSTLLAEGEKIGSSNGEVVPVTSTGAVDYSKAGLFGLLDFFARRTCLTVSGQLNVETHACALGDVYTFGPTFRAENSHTSRHLAEFWMIEPEVAFADLKDDIDLAEDYLKYLVAYALAHCQGDLEYFESCPGGEPGLRKRLQNVLENDFVRMTYTELVELVRKDA--KEGKVRI 1938
            AVV  A+    AP  FRR R+RV+ELL SG  L GQ   VKGW +T R A  G   FL ++DGSC    + V+   E  GF A    GGVGAS SV G VV S  KGQEVE+KA +V V G +  PD    ++TYP++KK H+LE LR  AHLRPR                  S +H AAMRVR+AMAFATH+FFN RGFLYIHTPI+T +DCEGAGE F V+TLL++       +G++ P+T    VDYSK       DFF R + L+VSGQLNVETH CAL DVYTFGPTFRAENS+TSRHLAEFWMIEPE+AFADLKDDIDLAEDYLKY V YAL HC  DLE+F      E GL +RL   +E+ FVR+TYT+ VEL+ K    K+GK  +
Sbjct:    3 AVVPSAKPTT-APTLFRRNRIRVKELLQSGLELEGQTVTVKGWAKTLREAGAGAFAFLELNDGSCFAGVQCVINKEETEGFAAAIASGGVGASFSVEGVVVKSPAKGQEVELKAAKVMVYGGI--PD----SATYPMSKKRHTLEHLRAHAHLRPR------------------SNIHGAAMRVRNAMAFATHEFFNQRGFLYIHTPIITESDCEGAGEMFGVTTLLSQ-----HPDGKL-PLTKENKVDYSK-------DFFDRPSYLSVSGQLNVETHCCALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEIAFADLKDDIDLAEDYLKYCVQYALDHCTEDLEFFNK--NVEKGLLERLAATVESPFVRITYTDAVELLLKPEHLKKGKFSV 358