mRNA_H-elongata_contig86744.16234.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig86744.16234.1
Unique NamemRNA_H-elongata_contig86744.16234.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig86744.16234.1 vs. uniprot
Match: D7FJC9_ECTSI (6PF2K domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FJC9_ECTSI)

HSP 1 Score: 92.0 bits (227), Expect = 1.830e-20
Identity = 42/52 (80.77%), Postives = 48/52 (92.31%), Query Frame = 1 
Query:    1 LVCVETVLTDDQKVWENVKDAKLNSPDYKGVAPEEVMKDFFKRIAAYKSVYE 156
            LVCVETVLTD++KVW+NVK+AKL SPDY G+ PEE M+DFFKRIAAYKSVYE
Sbjct:  265 LVCVETVLTDEKKVWDNVKEAKLKSPDYTGMTPEEAMEDFFKRIAAYKSVYE 316
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BLAST of mRNA_H-elongata_contig86744.16234.1 vs. uniprot
Match: A0A6A0GWU4_HYAAZ (6PF2K domain-containing protein n=1 Tax=Hyalella azteca TaxID=294128 RepID=A0A6A0GWU4_HYAAZ)

HSP 1 Score: 57.0 bits (136), Expect = 1.420e-8
Identity = 25/52 (48.08%), Postives = 35/52 (67.31%), Query Frame = 1 
Query:    1 LVCVETVLTDDQKVWENVKDAKLNSPDYKGVAPEEVMKDFFKRIAAYKSVYE 156
            L  +E++  D Q V  N+K+ K+N PDYKGV PE+ + DF +RI  YK +YE
Sbjct:  110 LFFIESICDDAQLVEANIKEVKVNGPDYKGVKPEKALADFLQRIEHYKRIYE 161
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BLAST of mRNA_H-elongata_contig86744.16234.1 vs. uniprot
Match: A0A8B7NQH9_HYAAZ (6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase-like n=2 Tax=Hyalella azteca TaxID=294128 RepID=A0A8B7NQH9_HYAAZ)

HSP 1 Score: 57.0 bits (136), Expect = 3.940e-8
Identity = 25/52 (48.08%), Postives = 35/52 (67.31%), Query Frame = 1 
Query:    1 LVCVETVLTDDQKVWENVKDAKLNSPDYKGVAPEEVMKDFFKRIAAYKSVYE 156
            L  +E++  D Q V  N+K+ K+N PDYKGV PE+ + DF +RI  YK +YE
Sbjct:  134 LFFIESICDDAQLVEANIKEVKVNGPDYKGVKPEKALADFLQRIEHYKRIYE 185
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BLAST of mRNA_H-elongata_contig86744.16234.1 vs. uniprot
Match: A0A7S4J1Y0_9STRA (Hypothetical protein n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4J1Y0_9STRA)

HSP 1 Score: 53.9 bits (128), Expect = 4.850e-7
Identity = 24/51 (47.06%), Postives = 36/51 (70.59%), Query Frame = 1 
Query:    4 VCVETVLTDDQKVWENVKDAKLNSPDYKGVAPEEVMKDFFKRIAAYKSVYE 156
            + VE++  D++ +  N+K  KL++PDYKG+ PEE +KDF KR   Y +VYE
Sbjct:  163 IFVESICDDEKILEANIKTVKLSTPDYKGIDPEEAIKDFRKRREQYMNVYE 213
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BLAST of mRNA_H-elongata_contig86744.16234.1 vs. uniprot
Match: A0A6A7G6N2_9CRUS (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-like isoform X1 n=2 Tax=Hirondellea gigas TaxID=1518452 RepID=A0A6A7G6N2_9CRUS)

HSP 1 Score: 52.8 bits (125), Expect = 1.240e-6
Identity = 22/52 (42.31%), Postives = 34/52 (65.38%), Query Frame = 1 
Query:    1 LVCVETVLTDDQKVWENVKDAKLNSPDYKGVAPEEVMKDFFKRIAAYKSVYE 156
            L  +E++  D+  V  N+K+ KLN PDY+G+ PE+ + DF +RI  Y+  YE
Sbjct:  134 LFFIESICDDETLVQSNIKEVKLNGPDYRGMNPEDALNDFLQRIRHYEENYE 185
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BLAST of mRNA_H-elongata_contig86744.16234.1 vs. uniprot
Match: C3YAF1_BRAFL (6PF2K domain-containing protein (Fragment) n=23 Tax=Branchiostoma TaxID=7737 RepID=C3YAF1_BRAFL)

HSP 1 Score: 51.2 bits (121), Expect = 4.350e-6
Identity = 22/49 (44.90%), Postives = 34/49 (69.39%), Query Frame = 1 
Query:   10 VETVLTDDQKVWENVKDAKLNSPDYKGVAPEEVMKDFFKRIAAYKSVYE 156
            +E++  D + V ENVK+ K+NSPDY+ +  +E M+DF +RI  Y+  YE
Sbjct:  153 IESMCDDPEVVAENVKEVKVNSPDYRNMNKDEAMRDFLERIRMYQKSYE 201
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BLAST of mRNA_H-elongata_contig86744.16234.1 vs. uniprot
Match: A0A7J6A716_AMEME (6PF2K domain-containing protein n=4 Tax=Ictaluridae TaxID=7996 RepID=A0A7J6A716_AMEME)

HSP 1 Score: 48.5 bits (114), Expect = 3.900e-5
Identity = 20/48 (41.67%), Postives = 32/48 (66.67%), Query Frame = 1 
Query:   10 VETVLTDDQKVWENVKDAKLNSPDYKGVAPEEVMKDFFKRIAAYKSVY 153
            +E++  D + +  N+K+ KLNSPDY+    ++V++DF KRI  YK  Y
Sbjct:  160 IESICDDPEIIEANIKEVKLNSPDYETCDKDDVLEDFLKRIECYKMTY 207
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BLAST of mRNA_H-elongata_contig86744.16234.1 vs. uniprot
Match: A0A8M2B8X9_DANRE (6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X2 n=3 Tax=Cyprinoidei TaxID=30727 RepID=A0A8M2B8X9_DANRE)

HSP 1 Score: 48.1 bits (113), Expect = 5.310e-5
Identity = 23/48 (47.92%), Postives = 30/48 (62.50%), Query Frame = 1 
Query:   10 VETVLTDDQKVWENVKDAKLNSPDYKGVAPEEVMKDFFKRIAAYKSVY 153
            VE++  D + + EN+K  KLNSPDY     EE ++DF KRI  YK  Y
Sbjct:  151 VESLCDDPEIIAENIKQVKLNSPDYFDCDKEEAVEDFLKRIDCYKLTY 198
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BLAST of mRNA_H-elongata_contig86744.16234.1 vs. uniprot
Match: A0A7M7N2D4_STRPU (6PF2K domain-containing protein n=1 Tax=Strongylocentrotus purpuratus TaxID=7668 RepID=A0A7M7N2D4_STRPU)

HSP 1 Score: 48.1 bits (113), Expect = 5.340e-5
Identity = 20/49 (40.82%), Postives = 31/49 (63.27%), Query Frame = 1 
Query:   10 VETVLTDDQKVWENVKDAKLNSPDYKGVAPEEVMKDFFKRIAAYKSVYE 156
            VE+V  D + +  N+++ KLNSPDY  +  E  ++DF +RI  YK  Y+
Sbjct:  172 VESVCDDPETIQSNIREVKLNSPDYYDIDKERAIRDFLQRIEHYKETYQ 220
[Close]
BLAST of mRNA_H-elongata_contig86744.16234.1 vs. uniprot
Match: F1QPT5_DANRE (Si:dkey-96f10.1 n=4 Tax=Danio rerio TaxID=7955 RepID=F1QPT5_DANRE)

HSP 1 Score: 48.1 bits (113), Expect = 5.340e-5
Identity = 23/48 (47.92%), Postives = 30/48 (62.50%), Query Frame = 1 
Query:   10 VETVLTDDQKVWENVKDAKLNSPDYKGVAPEEVMKDFFKRIAAYKSVY 153
            VE++  D + + EN+K  KLNSPDY     EE ++DF KRI  YK  Y
Sbjct:  144 VESLCDDPEIIAENIKQVKLNSPDYFDCDKEEAVEDFLKRIDCYKLTY 191
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig86744.16234.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 14
Match NameE-valueIdentityDescription
D7FJC9_ECTSI1.830e-2080.776PF2K domain-containing protein n=2 Tax=Ectocarpus... [more]
A0A6A0GWU4_HYAAZ1.420e-848.086PF2K domain-containing protein n=1 Tax=Hyalella a... [more]
A0A8B7NQH9_HYAAZ3.940e-848.086-phosphofructo-2-kinase/fructose-2, 6-bisphosphat... [more]
A0A7S4J1Y0_9STRA4.850e-747.06Hypothetical protein n=1 Tax=Odontella aurita TaxI... [more]
A0A6A7G6N2_9CRUS1.240e-642.316-phosphofructo-2-kinase/fructose-2,6-bisphosphata... [more]
C3YAF1_BRAFL4.350e-644.906PF2K domain-containing protein (Fragment) n=23 Ta... [more]
A0A7J6A716_AMEME3.900e-541.676PF2K domain-containing protein n=4 Tax=Ictalurida... [more]
A0A8M2B8X9_DANRE5.310e-547.926-phosphofructo-2-kinase/fructose-2, 6-bisphosphat... [more]
A0A7M7N2D4_STRPU5.340e-540.826PF2K domain-containing protein n=1 Tax=Strongyloc... [more]
F1QPT5_DANRE5.340e-547.92Si:dkey-96f10.1 n=4 Tax=Danio rerio TaxID=7955 Rep... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig86744contigH-elongata_contig86744:166..1829 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score93.2
Seed ortholog evalue3.8e-17
Seed eggNOG ortholog2880.D7FJC9
Preferred namePFKFB3
KEGG rclassRC00152
KEGG koko:K01103,ko:K19028,ko:K19029,ko:K19030
KEGG ReactionR02731
KEGG Pathwayko00051,ko04066,ko04152,ko04919,ko04922,map00051,map04066,map04152,map04919,map04922
GOsGO:0000166,GO:0002791,GO:0002793,GO:0003674,GO:0003824,GO:0003873,GO:0004331,GO:0005488,GO:0005515,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005829,GO:0005975,GO:0005996,GO:0006000,GO:0006003,GO:0006006,GO:0006007,GO:0006082,GO:0006089,GO:0006090,GO:0006091,GO:0006094,GO:0006096,GO:0006109,GO:0006110,GO:0006139,GO:0006140,GO:0006163,GO:0006164,GO:0006165,GO:0006725,GO:0006732,GO:0006733,GO:0006753,GO:0006754,GO:0006757,GO:0006793,GO:0006796,GO:0006807,GO:0006950,GO:0007275,GO:0007399,GO:0007417,GO:0007420,GO:0008144,GO:0008150,GO:0008152,GO:0008443,GO:0009056,GO:0009058,GO:0009108,GO:0009117,GO:0009123,GO:0009124,GO:0009126,GO:0009127,GO:0009132,GO:0009135,GO:0009141,GO:0009142,GO:0009144,GO:0009145,GO:0009150,GO:0009152,GO:0009156,GO:0009161,GO:0009165,GO:0009166,GO:0009167,GO:0009168,GO:0009179,GO:0009185,GO:0009199,GO:0009201,GO:0009205,GO:0009206,GO:0009259,GO:0009260,GO:0009605,GO:0009719,GO:0009725,GO:0009743,GO:0009746,GO:0009749,GO:0009889,GO:0009890,GO:0009891,GO:0009892,GO:0009893,GO:0009894,GO:0009895,GO:0009896,GO:0009987,GO:0009991,GO:0010033,GO:0010243,GO:0010562,GO:0010563,GO:0010646,GO:0010647,GO:0010675,GO:0010676,GO:0010677,GO:0010817,GO:0010906,GO:0014070,GO:0014074,GO:0016051,GO:0016052,GO:0016053,GO:0016301,GO:0016310,GO:0016311,GO:0016740,GO:0016772,GO:0016773,GO:0016787,GO:0016788,GO:0016791,GO:0017076,GO:0017144,GO:0018130,GO:0019200,GO:0019203,GO:0019219,GO:0019220,GO:0019222,GO:0019318,GO:0019319,GO:0019320,GO:0019359,GO:0019362,GO:0019363,GO:0019438,GO:0019439,GO:0019637,GO:0019693,GO:0019752,GO:0019899,GO:0019900,GO:0019901,GO:0023051,GO:0023056,GO:0030554,GO:0030808,GO:0030809,GO:0030810,GO:0030811,GO:0030812,GO:0030813,GO:0031099,GO:0031100,GO:0031323,GO:0031324,GO:0031325,GO:0031326,GO:0031327,GO:0031328,GO:0031329,GO:0031330,GO:0031331,GO:0031667,GO:0031960,GO:0031974,GO:0031981,GO:0032024,GO:0032501,GO:0032502,GO:0032553,GO:0032555,GO:0032559,GO:0032787,GO:0032868,GO:0032879,GO:0032880,GO:0032991,GO:0033131,GO:0033133,GO:0033674,GO:0033762,GO:0033993,GO:0034284,GO:0034404,GO:0034641,GO:0034654,GO:0034655,GO:0035639,GO:0036094,GO:0042221,GO:0042325,GO:0042326,GO:0042327,GO:0042578,GO:0042594,GO:0042802,GO:0042866,GO:0043085,GO:0043167,GO:0043168,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043434,GO:0043436,GO:0043467,GO:0043470,GO:0043540,GO:0043549,GO:0044093,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044262,GO:0044270,GO:0044271,GO:0044281,GO:0044282,GO:0044283,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044445,GO:0044446,GO:0044464,GO:0045820,GO:0045821,GO:0045912,GO:0045913,GO:0045934,GO:0045935,GO:0045936,GO:0045937,GO:0045980,GO:0045981,GO:0046031,GO:0046034,GO:0046364,GO:0046365,GO:0046390,GO:0046394,GO:0046434,GO:0046483,GO:0046496,GO:0046683,GO:0046700,GO:0046835,GO:0046883,GO:0046887,GO:0046939,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048545,GO:0048731,GO:0048856,GO:0050308,GO:0050708,GO:0050714,GO:0050789,GO:0050790,GO:0050794,GO:0050796,GO:0050896,GO:0051046,GO:0051047,GO:0051049,GO:0051050,GO:0051171,GO:0051172,GO:0051173,GO:0051174,GO:0051186,GO:0051188,GO:0051193,GO:0051194,GO:0051195,GO:0051196,GO:0051197,GO:0051198,GO:0051222,GO:0051223,GO:0051338,GO:0051347,GO:0051384,GO:0051591,GO:0055086,GO:0060322,GO:0061695,GO:0062012,GO:0062013,GO:0062014,GO:0065007,GO:0065008,GO:0065009,GO:0070013,GO:0070095,GO:0070201,GO:0071704,GO:0072330,GO:0072521,GO:0072522,GO:0072524,GO:0072525,GO:0080090,GO:0090087,GO:0090276,GO:0090277,GO:0090407,GO:0097159,GO:0097367,GO:1900371,GO:1900372,GO:1900373,GO:1900542,GO:1900543,GO:1900544,GO:1901135,GO:1901137,GO:1901265,GO:1901292,GO:1901293,GO:1901360,GO:1901361,GO:1901362,GO:1901363,GO:1901564,GO:1901566,GO:1901575,GO:1901576,GO:1901615,GO:1901652,GO:1901698,GO:1901700,GO:1902494,GO:1903299,GO:1903301,GO:1903530,GO:1903532,GO:1903578,GO:1903579,GO:1903580,GO:1904951,GO:1990234,GO:2001169,GO:2001170,GO:2001171
EggNOG free text desc.6phosphofructo2kinase
EggNOG OGsCOG0406@1,KOG0234@2759
EC2.7.1.105,3.1.3.46
COG Functional cat.G
BiGG ReactioniMM904.YJL155C,iND750.YJL155C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Hectar predicted targeting categoryno signal peptide or anchor
Exons2
Model size156
Cds size156
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931268.6930807-CDS-H-elongata_contig86744:165..2071622931268.6930807-CDS-H-elongata_contig86744:165..207Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig86744 166..207 -
1691679766.9792583-CDS-H-elongata_contig86744:165..2071691679766.9792583-CDS-H-elongata_contig86744:165..207Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig86744 166..207 -
1622931268.716964-CDS-H-elongata_contig86744:1715..18291622931268.716964-CDS-H-elongata_contig86744:1715..1829Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig86744 1716..1829 -
1691679766.9938731-CDS-H-elongata_contig86744:1715..18291691679766.9938731-CDS-H-elongata_contig86744:1715..1829Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig86744 1716..1829 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig86744.16234.1prot_H-elongata_contig86744.16234.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig86744 166..1829 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig86744.16234.1

>prot_H-elongata_contig86744.16234.1 ID=prot_H-elongata_contig86744.16234.1|Name=mRNA_H-elongata_contig86744.16234.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=52bp
LVCVETVLTDDQKVWENVKDAKLNSPDYKGVAPEEVMKDFFKRIAAYKSV
YE
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mRNA from alignment at H-elongata_contig86744:166..1829-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig86744.16234.1 ID=mRNA_H-elongata_contig86744.16234.1|Name=mRNA_H-elongata_contig86744.16234.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1664bp|location=Sequence derived from alignment at H-elongata_contig86744:166..1829- (Himanthalia elongata Himel1 dioecious)
CTGGTGTGCGTGGAAACGGTGCTGACGGATGATCAAAAAGTGTGGGAAAA
CGTGAAGGACGCGAAGCTCAACAGCCCGGACTACAAGGGCGTGGCTCCCG
AGGAGGTCATGAAGGTGGGGGTGCCGACGACGAGGGGCCACGGCTAAACA
TCTCGAGTCAATCCCGACCCCCGAAATTCCATGGAGCTTTCTCTTTTTCT
TCGACAACGACACGATCGAAGTATTACTGAGCTTGAAACGCCGTGGGAGT
ATTGTTATGATGTTGGGGGTATTAAGATGTCGGAGATACTATGATGTTGA
ACTGGCTTTGGAAACCCCGAGAAGCGTATTCGGTCCCTCAATCTTCCTGC
CTTCCTTAGACGTGGCGTGGCGTGCCTTGGCTTGGCTTGGCTTGACTTGA
CATGGTAAAAGTGTAATACCGGCCGTTAATATTGTAGAACAAATGACAAC
CCCCAACGCTAGTTACACTCATTTTTTTTCTCTTAGTCGATGGTCCGTTG
AACGTCGCACTCTCAACTACCGCACGCGTTGATCCGGCCTGTGGTCGACT
ACGGGGATTCTCCCACCGCTCCTCTGTTTTTGTCGTCAGTGTGGTTACTC
TTAGTCTTACTCTTACTCTTACTCTTAACTAGTACTTTTGAATAGCGATG
CATGTTTAGCGCACTCCTAACTGGATCTTATTTACTCTTCTAGTACCAGG
TATAGTATAGTATAGTATAGTACAGTATAGTATAGTATAGTATAGTATAG
TATAGTATAGTATAGTATAGTACATTATAGTATAGTATAGTATAGTATAG
TATAGTATAGTACAGTATAGTATAGTATAGTATAGTACATTATCGTATAG
TATAGTATAGTATAGTATAGTACGTTAAAGTATAGTATAGTATAGTACAT
TATAGTATAGTATAGTATAGTATAGTATAGTATAGTACATTATAGTATAG
TATAGTATAGTATAGTACATTATAGTATAGTATAGTATAGTACGTTAAAG
TATAGTATAGTATAGTATAGTACGTTAAAGTATAGTATAGTATAGTATAG
TACATTAAAGTATAGTAAAGTATAGTATAGTATATTATAGTAACCAGCTG
CTCTGCGCTATCCTATTGGATCAGACCACAGTACAGTACCTTTAGTGCCT
CTTGCTTTTCGAGTGCTGCAGGCACCCCTCCTCCCCCCTGCTGATGTACT
GTACAGTAGATATGCACCCTACGGGACGCTACCTCATGCTGTAGGTGATC
CCCCCCTCTCTCTCTCTCTCTCTCTCTCTCAGTTCCTACACTTTGATCAT
CCAACCTCAGTACCCCCCCCCATACCAGCTCCTGCATTTGGACTGGACAC
TATACTAGAGAGTTGATAATAAAACACGTGGCGTGATGCTGCAAGTGCCT
TTTTTCCCCTTCCTTCCCTCTCTCCCTCCCCTCAGTGGTGTGCCTCGAGT
CCCCCCCCCCCCCTCCCCTCCCCCTCCCCCTCCTTCCGCGCTTTTTTTAC
GATACCGCGTGCTTCTGTGTTTTCTTCATCGCTTTACCCTACCGACTGCT
CACTGCTCTATATCATTGCGTTGTGCTGTTGCTTCTACTGTCTACGTATG
CCTTTCGTGTGACACGGGACAGGACTTTTTCAAGAGGATAGCGGCCTATA
AGAGCGTCTACGAG
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Coding sequence (CDS) from alignment at H-elongata_contig86744:166..1829-

>mRNA_H-elongata_contig86744.16234.1 ID=mRNA_H-elongata_contig86744.16234.1|Name=mRNA_H-elongata_contig86744.16234.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=312bp|location=Sequence derived from alignment at H-elongata_contig86744:166..1829- (Himanthalia elongata Himel1 dioecious)
CTGGTGTGCGTGGAAACGGTGCTGACGGATGATCAAAAAGTGTGGGAAAA
CGTGAAGGACGCGAAGCTCAACAGCCCGGACTACAAGGGCGTGGCTCCCG
AGGAGGTCATGAAGCTGGTGTGCGTGGAAACGGTGCTGACGGATGATCAA
AAAGTGTGGGAAAACGTGAAGGACGCGAAGCTCAACAGCCCGGACTACAA
GGGCGTGGCTCCCGAGGAGGTCATGAAGGACTTTTTCAAGAGGATAGCGG
CCTATAAGAGCGTCTACGAGGACTTTTTCAAGAGGATAGCGGCCTATAAG
AGCGTCTACGAG
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