mRNA_H-elongata_contig8179.15847.1 (mRNA) Himanthalia elongata Himel1 dioecious

BLAST of mRNA_H-elongata_contig8179.15847.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 584 bits (1506), Expect = 7.600e-187
Identity = 339/679 (49.93%), Postives = 441/679 (64.95%), Query Frame = 2

Query:    2 GLDHLLASGKGPATSGD---TDLHMACFMSLVSYLASRAEVLRVAPYHRKTILNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQIIDSGLDQDSCFFRDSDGQNVEGGYYFDEIAIIS---STEVFSWFDSGGSFPVDLDRRKIVQYISLVKPEDEDETYIFTSSGQQIHWYDAEGFAVDTAGGHGSHTAGTAAGSTVTSPVQLGTCGGDDELGCLGGCLNTSYVEQLLSNQYV-----DIDVLCPKFDCDTLGTDFETCLSDDVAETLSANEGVAPGAKLAIFDV--SVDGIVIWAELAGNGLWEASNGTGSVVHSNSWGGVSDCTVDDASILFDTYMYKNPEHLLLFAAGNDGDPSDTTCTVGSPAVAKNVLAVGASSSGETRWTSTGDDGEQILTIDQTWSDIDTVSFFSSRGPTEDDRIKPELVAPGDRVLSVSSSGDDGETCKLELQVGSSMAVPVVAAGAAMIRQYFKNDDFYTTDLETRGLCGGNSTYSTFVCSPFNASAATVKAMLINSANLMGGSSEPDGSRGFGRVHLEGGLPLGGLGDVGLFVADSSNTNVTQGAPDYYTFELLDGDAG-EFRATLVWIDPPATTISKNQLIHDLDLTVSSPSGQTYTMWSSNKADTSNVIERVIVSVEDFVAEDNGTWTVSVTANGLMTESQPYSLVVTGPFGNG 1996
            GLDHLL SG+G   S +   T+LH+ACFM LVS+ A R EVLRV+P H K +LN  A+ I  SA+   ++T LT AGLDGTGEVIQ++D+GLD+ SCFF D  G+ V+ GY+F+E+ + S   S  ++      G F    +RRK++QYI LV P D D     T+ G    +   + F  D + GHG+HTAG+AAG+T+  P +  TC GD+ LGC+GGC++ S     L +  +     D+D LCP + CD   TD   CLSD+V ETL+ N G+A GAKL+IFDV   V G++   E+ GNG+WE     G  VHS S GG + CT  ++ IL+D +MY+NPE+LL+FAAGNDG+   + CT+ SPA+ KNVLAVGAS+SG+TR  ST           +  + ID V+ FSS G T D RIKPE+VAPGD V S +S G D  +C+L    G+SM+ P+VA  AAM+RQYF ++ FYT D+  RG C        F+C  F+ SAATVKA+LINSANLMG SS+PD  RGFGR+HLE G+PL G GD+ LFVAD+ +T++ +     Y F++ D DAG E RATL WID PAT+ S  QL++DLDL V SPSG  Y MW S+  D  NV ERVIV  +D    + GTWTV V +N L+T+SQ YSLVV G    G
Sbjct:  293 GLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASA--TDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQLIYYPVFEAGDFTYTEERRKVIQYIELVNP-DSDYVAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDG-STD--PCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLI---EVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETERSVCTMASPAIGKNVLAVGASTSGDTRVIST-----------EGGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDG-----FICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDV-DADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDV---ETGTWTVLVYSNSLVTDSQSYSLVVNGAISAG 942          

BLAST of mRNA_H-elongata_contig8179.15847.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 562 bits (1449), Expect = 6.680e-182
Identity = 342/690 (49.57%), Postives = 434/690 (62.90%), Query Frame = 2

Query:    2 GLDHLLASGKGPATSGD---TDLHMACFMSLVSYLASRAEVLRVAPYHRKTILNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQIIDSGLDQDSCFFRDSDGQNVEGGYYFDEIA-------IISSTEVFSWFDSGGSFPVDLDRRKIVQYISLVKPEDEDETYIFTSSGQQIHWY---------DAEGFAVDTAGGHGSHTAGTAAGSTVTSPVQLGTCGGDDELGCLGGCLNTSYV-EQLLSNQY----VDIDVLCPKFDCDTLGTDFETCLSDDVAETLSANEGVAPGAKLAIFDVSVDGIVIWAELAGNGLWEASNGTGSVVHSNSWGGVSDCTVDDASILFDTYMYKNPEHLLLFAAGNDGDPSDTTCTVGSPAVAKNVLAVGASSSGETRWTSTGDDGEQILTIDQTWSDIDTVSFFSSRGPTEDDRIKPELVAPGDRVLSVSSSGDDGETCKLELQVGSSMAVPVVAAGAAMIRQYFKNDDFYTTDLETRGLCGGNSTYSTFVCSPFNASAATVKAMLINSANLMGGSSEPDGSRGFGRVHLEGGLPLGGLGDVGLFVADSSNTNVTQGAPDYYTFELLDGDAG-EFRATLVWIDPPATTISKNQLIHDLDLTVSSPSGQTYTMWSSNKADTSNVIERVIVSVEDFVAEDNGTWTVSVTANGLMTESQPYSLVVTGPFGNG 1996
            GLDHLL SG+G   S +   T+LH+ACFM LVS LA R EVLRV+P   K +LNA A+ I  SA+   ++T LT AGLDGTGE+IQ+IDSGLD+ SC+F D  G+ V+ GYYFDE+A       + SS E  + F+ GG F    +RRKI+QYI LV P+    ++ +TS G  I ++         D    A+       +    +AAG+T+ +P +  TC   D L C+G C++   V + L+S  +     D+D LCP + CD   TD  TCLSDDV ETLS N G+A GAKLAIFDV   G    +   GN LWE     G  +HSNS G   +C++    IL D +MY+N EHLL+FAAGN+G    +TCT+ +P + KN LA+GAS+SG+TR          I T+  T   I+TV+ FSS G T DDRIKPE+VAPGD V S +S G D  +C+L    G+SM+ P+VA  AAMIRQYF ++ FY  D+  RG C        F+C  F+ SAATVKA+LINSANLMGGSSEPDG RGFGRVHLE G+PL G G++ LFVAD+ +T++ +G  D Y F++ D DAG + RATL WIDPP+T  S  QL+H+LDL V SP G TY MW S   D  NV ERVIV   D V    GTWTV V AN L+T+SQ YSLVV G    G
Sbjct:    5 GLDHLLFSGRGAGGSHEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASA--TDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELAQAAVYSSLYSSDETATIFN-GGDFSYYPERRKIIQYIDLVNPDSPYSSF-YTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACIDLLDVTDDLVSISHQTGGTDLDRLCPMYGCDN-STD-TTCLSDDVPETLSNNGGMAQGAKLAIFDVFY-GDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGEERSTCTINTPGIGKNSLAIGASTSGDTR---------VISTVGATG--INTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCS-----DVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDV-DADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVVT---GTWTVWVWANSLITDSQSYSLVVNGAISPG 667          

BLAST of mRNA_H-elongata_contig8179.15847.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 569 bits (1467), Expect = 3.420e-180
Identity = 337/713 (47.27%), Postives = 448/713 (62.83%), Query Frame = 2

Query:    2 GLDHLLASGKGPATSGD----TDLHMACFMSLVSYLASRAEVLRVAPYHRKTILNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQIIDSGLDQDSCFF-RDSDGQNVEGGYYFDEIAI---ISSTEVFS----------W------------------FDSGGSFPVDLDRRKIVQYISLVKPEDEDETY---IFTSSGQQIHWYDAEGFAVDTAGGHGSHTAGTAAGSTVTSPVQLGTCGGDDELGCLGGCLN---TSYVEQLLSN--QYVDIDVLCPKF---DCDTLGTDFETCLSDDVAETLSANEGVAPGAKLAIFDVSVDGIVIWAELAGNGLWEASNGTGSVVHSNSWGGVSDCTVDDASILFDTYMYKNPEHLLLFAAGNDGDPSDTTCTVGSPAVAKNVLAVGASSSGETRWTSTGDDGEQILTIDQTWSDIDTVSFFSSRGPTEDDRIKPELVAPGDRVLSVSSSGD-DGETCKLELQVGSSMAVPVVAAGAAMIRQYFKNDDFYTTDLETRGLCGGNSTYSTFVCSPFNASAATVKAMLINSANLMGGSSEPDGSRGFGRVHLEGGLPLGGLGDVGLFVADSSNTNVTQGAPDYYTFELLDGDAGEFRATLVWIDPPATTISKNQLIHDLDLTVSSPSGQTYTMWSSNKADTSNVIERVIVSVEDFVAEDNGTWTVSVTANGLMTESQPYSLVVTGPFGNG 1996
            GLDHLL SG+G     D     +LH+ACFM LVS+LAS+ EVLRVAP   + +LNA A+A   SAT   ++T LT AGLDGTG+V+Q+IDSG+D+ SCFF  D  G +V  G+YF+E  I   ++S+   S          W                  FD GG F +  DRRKI+QYI+L+K +D + ++     TS GQQ  W  A+ F  D   GHG+H AG+ AG+T+ +P +L +C   + + C+G C++    + V+ L +   + VD+D LCP F   DCD L T  E CLSDDVAETL+ N G+A GAKLAIFD   D   + +   GNGLWE        +HSNS+GG  +C++    +L+D +M  NPE+LL+FAAGNDG+   +TCT+ SPA+AKNVLAVGAS+SG+TR ++T   G            IDTV+ FSS GPT D RIKPE++APGD + S +S G  D  +C+L    G+SM+ P+VA  +AMIRQYF N+ F+ TD+  RG C   +      C  F+ S+AT+KA++INSANLMGGSSEPDGSRGFGR+HLE G+PL G G + LFV+DS+ T++ +     Y F++      + R TL W+DPPATT S  QL+HDLDL V SP+G  YTMW+S   D++NV ERVIV   D    + GTW+V + A+ L T+ Q YSLVV G    G
Sbjct:  283 GLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATV--TDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFD-GGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFSDYDCDVLET--EKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGL-SPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATPGLG------------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG-----CEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDV---ETGTWSVWIWADNLTTDVQSYSLVVNGAISPG 969          

BLAST of mRNA_H-elongata_contig8179.15847.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 558 bits (1437), Expect = 2.020e-176
Identity = 342/754 (45.36%), Postives = 448/754 (59.42%), Query Frame = 2

Query:    2 GLDHLLASGKGPATSGDTDLHMACFMSLVSYLASRAEVLRVAPYHRKTILNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQIIDSGLDQDSCFFRDSDGQNVEGGYYFDEIAIISSTEVFSWFDSGGSFPVDLDRRKIVQYISLVKPEDEDET--------YIFTSSGQQIHWYDAE-----------------------------------------------------GFAVDTAGGHGSHTAGTAAGS-TVTSPVQLGTCGGDDELGCLGGCLNTSYVEQLLSNQYVDIDVLCPKFDCDTLGTDFET--CLSDDVAETLSANEGVAPGAKLAIFDVSVDGIVIWAELAGNGLWEASNGTGSVVHSNSWGGVSDCTVDDASILFDTYMYKNPEHLLLFAAGNDGD----PSDTTCTVGSPAVAKNVLAVGASSSGETRWTSTGDDG----EQILTIDQT-----W----------------SDIDTVSFFSSRGPTEDDRIKPELVAPGDRVLSVSSSGDDGETCKLELQVGSSMAVPVVAAGAAMIRQYFKNDDFYTTDLETRGLCGGNSTYSTFVCSPFNASAATVKAMLINSANLMGGSSEPDGSRGFGRVHLEGGLPLGGLGDVGLFVADSSNTNVTQGAPDYYTFELLDGDAG-EFRATLVWIDPPATTISKNQLIHDLDLTVSSPSGQTYTMWSSNKADTSNVIERVIVSVEDFVAEDNGTWTVSVTANGLMTESQPYSLVVTG 1981
            G++HLL   + P+     +   AC+M L+S LA++ EVLRV+P     + NAVA A+  SATT  +ET L  AGLDGTGEVIQ++DSGLD+ SCFF D DG  VE G+ FD + +     V S      SFP D+ RRKIVQYI L K  D+  T        Y+    G    + D+E                                                     GF  DT GGHG+ TAG+AAG+ +  S ++   C GD+  GC GGC++ S V+ +L +   D+DV CP + CD   TD  +  CLSDD  E L  + GVAPGA++++FDVS  G  I+A  AGN LW ++  TG+ +H+NSWGG + C + +   L+DT+MY+NPEHLL+FAAGNDG     P+   CTVGSPA+ KN LAVGA+SSG    T TGDDG    E++   D +     W                + +DTV+FFSS+GPT D RIKP++VAPGD+V S SS G DG +CKL   +G+S + P+VA  AA++RQYFK+  F+  D+ +RG C  +S  S+  CS F  SAATVKAM+INSA+LMGGSSEP+G RGFGRVHLE G+P+ G+G+ GL V +++   +  G  +      +DGDAG E RATL W+DPP T +S  QL HDLDL V +PSG  YTMW S   DT NV+ERVIV VE     ++G W+V V+A  L+T+ Q YSLVVTG
Sbjct:  277 GMNHLLPGKRFPS-----ETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATT--TETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNV-STVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDG-NTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHS--SSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAA---IADG--EITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESM---ESGVWSVVVSAKALLTDEQLYSLVVTG 1011          

BLAST of mRNA_H-elongata_contig8179.15847.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 548 bits (1412), Expect = 4.900e-176
Identity = 322/685 (47.01%), Postives = 427/685 (62.34%), Query Frame = 2

Query:   77 MSLVSYLASRAEVLRVAPYHRKTILNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQIIDSGLDQDSCFF-RDSDGQNVEGGYYFDEIAIISSTEVFSW--------------------------------FDSGGSFPVDLDRRKIVQYISLVKPEDEDETY---IFTSSGQQIHWYDAEGFAVDTAGGHGSHTAGTAAGSTVTSPVQLGTCGGDDELGCLGGCLNTSYVEQL--LSNQYV---DIDVLCPKF---DCDTLGTDFETCLSDDVAETLSANEGVAPGAKLAIFDVSVDGIVIWAELAGNGLWEASNGTGSVVHSNSWGGVSDCTVDDASILFDTYMYKNPEHLLLFAAGNDGDPSDTTCTVGSPAVAKNVLAVGASSSGETRWTSTGDDGEQILTIDQTWSDIDTVSFFSSRGPTEDDRIKPELVAPGDRVLSVSSSGD-DGETCKLELQVGSSMAVPVVAAGAAMIRQYFKNDDFYTTDLETRGLCGGNSTYSTFVCSPFNASAATVKAMLINSANLMGGSSEPDGSRGFGRVHLEGGLPLGGLGDVGLFVADSSNTNVTQGAPDYYTFELLDGDAGEFRATLVWIDPPATTISKNQLIHDLDLTVSSPSGQTYTMWSSNKADTSNVIERVIVSVEDFVAEDNGTWTVSVTANGLMTESQPYSLVVTGPFGNG 1996
            M LVS+LAS+ EVLRVAP   + +LNA A+A   SAT   ++T LT AGLDGTG+V+Q+IDSG+D+ SCFF  D  G +V  G+YF+E  I  S    S+                                FD GG F +  DRRKI+QYI+L+K +D + ++     TS GQQ  W  A+ F  D   GHG+H AG+ AG+T+ +P +L +C   + + C+G C++      +  L+  YV   D+D LCP F   DCD L TD   CLSDDVAETL+ N G+A GAKLAIFD   D   + +   G GLWE        +HSNS+GG  +C++    +L+D +M  NPE+LL+FAAGNDG+   +TCT+GSPA+AKNVLAVGAS+SG+TR ++T   G            IDTV+ FSS GPT D RIKPE++APGD + S +S G  D  +C+L   VG+SM+ P+VA  +AMIRQYF N+ F+ TD+  RG C   +      C  F+ S+AT+KA++INSANLMG SSEP+GSRGFGR+HLE G+PLGG G + LFV+DS+ T++ +     Y F++      + R TL W+DPPATT S  QL+HDLDL V SP+G  YTMW+S   D+SNV ERVIV  +D    + GTW+V + A+ L T+ Q YSLVV G    G
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATV--TDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFD-GGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDGLETD--KCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGL-SPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELRRSTCTIGSPAIAKNVLAVGASTSGDTRLSATPGSG------------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG-----CEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDV---ETGTWSVWIWADNLTTDVQSYSLVVNGAISPG 659          

BLAST of mRNA_H-elongata_contig8179.15847.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 551 bits (1421), Expect = 1.180e-174
Identity = 335/681 (49.19%), Postives = 419/681 (61.53%), Query Frame = 2

Query:    2 GLDHLLASGKGPATSGD---TDLHMACFMSLVSYLASRAEVLRVAPYHRKTILNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQIIDSGLDQDSCFFRDSDGQNVEGGYYFDEI-------AIISSTEVFSWFDSGGSFPVDLDRRKIVQYISLVKPEDEDETYIFTSSGQQIHWYDAEGFAVDTAGGHGSHTAGTAAGSTVTSPVQLGTCGGDDELGCLGGCLNT-SYVEQLLS----NQYVDIDVLCPKFDCDTLGTDFETCLSDDVAETLSANEGVAPGAKLAIFDVSVDGIVIWAELAGNGLWEASNGTGSVVHSNSWGGVSDCTVDDASILFDTYMYKNPEHLLLFAAGNDGDPSDTTCTVGSPAVAKNVLAVGASSSGETRWTSTGDDGEQILTIDQTWSDIDTVSFFSSRGPTEDDRIKPELVAPGDRVLSVSSSGDDGETCKLELQVGSSMAVPVVAAGAAMIRQYFKNDDFYTTDLETRGLCGGNSTYSTFVCSPFNASAATVKAMLINSANLMGGSSEPDGSRGFGRVHLEGGLPLGGLGDVGLFVADSSNTNVTQGAPDYYTFELLDGDAG-EFRATLVWIDPPATTISKNQLIHDLDLTVSSPSGQTYTMWSSNKADTSNVIERVIVSVEDFVAEDNGTWTVSVTANGLMTESQPYSLVVTGPFGNG 1996
            GLDHLL SG+G   S +   T+LH+ACFM LVS+LA R EVLRV+P   K +LNA A+ +  SA+   ++T LT AGLDGTGE+IQ+IDSGLD+ SC+F D  G+ V+ GYYFDE+       +  SS E  + F+ GG F    +RRK                                        GHG+HTAG+AAG+T+  P +  TC   D L C+G C++     + L+S        D+D LCP + CD   TD  TCLSDDV ETLS N G+A GAKLAIFDV   G    + + GN LWE     G  +HSNS G   +C++    IL D +MY+N E+LL+FAAGN+G    +TCT+ +P + KN LA+GAS+SG+TR          I T+  T   IDTV+ FSS G T DDRIKPE+VAPGD V S +S G D  +C+L    G+SM+ P+VA  AAMIRQYF ++ FYT D+  RG C        F+C  F+ SAATVKA+LINSANLMGGSSEPDG RGFGRVHLE G+PL G GD+ LFVAD+ +T++  G  + Y F++ D DAG + RATL WIDPPAT  S  QL+H+LDL V SP G TY MW S+  D  NV ERVIV   D V    GTWTV V AN L+T+SQ YSLVV G    G
Sbjct:  291 GLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASA--TDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELGRAAVYSSSYSSDETATIFN-GGDFSYYPERRKA---------------------------------------GHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDN-STD-TTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSI-GNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIERSTCTINTPGIGKNSLAIGASTSGDTR---------VISTVGATG--IDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDG-----FICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDV-DADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVT---GTWTVWVWANNLVTDSQSYSLVVNGAISPG 906          

BLAST of mRNA_H-elongata_contig8179.15847.1 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 545 bits (1404), Expect = 4.610e-172
Identity = 341/765 (44.58%), Postives = 446/765 (58.30%), Query Frame = 2

Query:    2 GLDHLLASGKGPATSGDTDLHMACFMSLVSYLASRAEVLRVAPYHRKTILNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQIIDSGLDQDSCFFRDSDGQNVEGGYYFDEIAIISSTEVFSWFDSGGSFPVDLDRRKIVQYISLVKPEDEDETYIFTSSGQQIHWY----------------DAE-------------------------------------------------------GFAVDTAGGHGSHTAGTAAGS-TVTSPVQLGTCGGDDELGCLGGCLNTSYVEQLLSNQYVDIDVLCPKFDCDTLGTDFET--CLSDDVAETLSANEGVAPGAKLAIFDVSVDGIVIWAELAGNGLWEASNGTGSVVHSNSWGGVSDCTVDDASILFDTYMYKNPEHLLLFAAGNDGD----PSDTTCTVGSPAVAKNVLAVGASSSGETRWTSTGDDG----EQILTIDQT-----W----------------SDIDTVSFFSSRGPTEDDRIKPELVAPGDRVLSVSSSGDDGETCKLELQVGSSMAVPVVAAGAAMIRQYFKNDDFYTTDLETRGLCGGNSTYSTFVCSPFNASAATVKAMLINSANLMGGSSEPDGSRGFGRVHLEGGLPLGGLGDVGLFVADSSNTNVTQGAPDYYT-FELLDGDAG-EFRATLVWIDPPATTISKNQLIHDLDLTVSSPSGQTYTMWSSNKADTSNVIERVIVSVEDFVAEDNGTWTVSVTANGLMTESQPYSLVVTG 1981
            G++HLL   K P+     +   AC+M L+S LA++ EVLRV+P    ++ NAVA A+  SATT  +ET L  AGLDGTGEVIQ++D+GLD+ SCFF D DG  VE G+ F  + +     V S      SFP D+ RRKIVQYI + K  D+  T    SSGQ    Y                D+E                                                       GF  D  GGHG+ TAG AAG+ +  S +Q   C GD+  GC GGC++ S V+ +L N   D+DV CP ++CD   TD  +  CLSDD  E L  + GVAPGA++++ D S  G   +A  AGN LW ++  TG+ +HSNSWG    C + +   L+D++MY+NPEHLL+FAAGN G     P+   CT+GSPA+ KN+LAVGA+SSG +R T TG+DG    E+    D +     W                + +DTV+FFSS+GPT D RIKP++VAPGD+V S SS G DG +C+L   +G+S + P+VA  AA++RQYFK+  F+  D+ +RG C  +S+ S   CS F  SAATVKAM+INSA+LMGGSSEP+G RGFGRVHLE G+P+ G+G+ GL V ++     T G+ D  T    +DGDAG E RATL W+DPP T +S  QL HDLDL V +PSG  YTMW S   DT NV+ERVIV VE     ++G W+V V+A GL+T+ Q YSLVVTG
Sbjct:  230 GMNHLLPGKKAPS-----EARTACYMGLLSQLAAKPEVLRVSPLQSASLYNAVANALVQSATT--TETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQVEHGFLFGGVRVEGDGNV-STVLGNYSFPFDMSRRKIVQYIQMFKTPDQGST----SSGQNSFTYASDYIRDYLGDAFDDDDSEYNSYDDYSGEYSSDTWSEESFCAQVLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDG-NTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQ--CSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEA-----TIGSDDEITELVTVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESM---ESGVWSVVVSAKGLLTDEQSYSLVVTG 971          

BLAST of mRNA_H-elongata_contig8179.15847.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 548 bits (1411), Expect = 3.320e-171
Identity = 347/743 (46.70%), Postives = 446/743 (60.03%), Query Frame = 2

Query:    2 GLDHLLASGKGPATSGDTD---LHMACFMSLVSYLASRAEVLRVAPYHRKTILNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQIIDSGLDQDSCFFRDSDG-QNVEGGYYFDEIAIISSTEVFSWFDS------------GGSFPVDLDRRKIVQYISLVKPEDED-------------------------------ETYIFTSSGQQIHWYDAEGFAVDTAGGHGSHTAGTAAGSTVTSPVQLGT------CGGDDELGCLGGCLN-----------TSYVEQLLSNQY--VDIDVLCPKFDCDTLGTDFETCLSDDVAETLSANEGVAPGAKLAIFDVSVDGIVIWAELAGNGLWEASNGTGSVVHSNSWGGVSDCTVDDASILFDTYMYKNPEHLLLFAAGNDGDPSDTTCTVGSPAVAKNVLAVGASSSGETRWTSTGDDGEQILTIDQTWSDIDTVSFFSSRGPTEDDRIKPELVAPGDRVLSVSSSGDDGETCKLELQVGSSMAVPVVAAGAAMIRQYFKNDDFYTTDLETRGLCGGNSTYSTFVCSPFNASAATVKAMLINSANLMGGSSEPDGSRGFGRVHLEGGLPLGGLGDVGLFVADSSNTNVTQGAPDYYTFELLDGDAG-EFRATLVWIDPPATTISKNQLIHDLDLTVSSPSGQTYTMWSSNKADTSNVIERVIVSVEDFV------------AEDNGTWTVSVTANGLM-TESQPYSLVVTGPFG 1990
            G+DHLL  G+G   +   +   L +ACFM+L+S+LASR EVLRV+P     +LNA A     SA+   +E+ +T AGLDGTGEVIQI+D+GLD +SCFF D DG + V  GYYF+EI + +S+   SWF S            GG F  DL RRK +  +    P  +                                E++  TS+G+Q  W  A  FA D AGGHG+HTAG+AAG+ V+SP    T      C    EL C+GGC++           T++  Q     Y  +D+D LCP+ DCD L  ++  CL DD AETL  + GVA GA+LAIFD+  D   +  ++AGNG+WE +   GS + SNSWG  + C      +LFD +MY+N + LLLFAAGNDGD   + CT+G+PA+AKNVLAVGAS+SG  R + T +DG+     D    DID +++FSS GPT D+RIKPE+VAPGD + S + SGD   TC+L    G+SM+ P+VA  AA++RQYFKN+ FY  D+E RG C       +F C PF  SAATVKAMLINSANLMGGS+EPDG RGFGRVHLE G+PL G GD  LFVADS+ T+  +     Y+F  +D  AG + RATL WIDPPATT S  QL+HDLDL+V SP+G  Y+MW     D+ N  ERVIV   + +             E +GTWTV V +  L   E+Q YSLVVTG  G
Sbjct:  352 GMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASL--TESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASS-FGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGL-VVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWA-TSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEY--CLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGDDW-SGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPT-DGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFC------DSFGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSF-AVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAIG 1078          

BLAST of mRNA_H-elongata_contig8179.15847.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 535 bits (1379), Expect = 4.300e-168
Identity = 339/738 (45.93%), Postives = 440/738 (59.62%), Query Frame = 2

Query:    2 GLDHLLASGK---GPATSGDTDLHMACFMSLVSYLASRAEVLRVAPYHRKTILNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQIIDSGLDQDSCFFRDSDGQNVEGGYYFDEIA-----IISSTEVFSWFDS---GGSFPVDLDRRKIVQYISLVKPEDEDETYIFTSSGQQIHWYDAEGFAVDTAGGHGSHTAGTAAGSTVTSPVQLGTCG-GDDELGCLGGCLNTSYVEQLLSNQYVDIDVLCPKFDCDTLGTDFETCLSDDVAETLSANEGVAPGAKLAIFDVSVDGIVIWAELAGNGLWEASNGTGSVVHSNSWGGVSDCTVDDASILFDTYMYKNPEHLLLFAAGNDGDPSDT-TCTVGSPAVAKNVLAVGASSSGETRWTSTGDDGEQILTIDQTWSDIDTVSFFSSRGPTEDDRIKPELVAPGDRVLSVSSSGDDGETCKLELQVGSSMAVPVVAAGAAMIRQYFKNDDFYTTDLETRGLCGGNSTYSTFVCSPFNASAATVKAMLINSANLMGGSSEPDGSRGFGRVHLEGGLPLGGLGDVGLFVADSSNTNVTQGAPDYYTFELLDGDAG-EFRATLVWIDPPATTISKNQLIHDLDLTVSSPSGQTYTMWSSNKADTSNVIERVIVSVEDFVAEDNGT--WTVSVTANGLMT-ESQPYSLVVTGPFGNGIIVNDASV------------------AAPLFHRGVVFLTSFFCAGVGLASALLAAS 2110
            GLDHLL  G+   G +     +L MAC+M LVS+LASR EVL+VA ++RK  LNA A+A   SA+ D+  T LT AGLDG+GEVIQIID+G+++ +CF    DG  VE GYYFD +A       SS+E  S F +   GG F  D DRRK++QYI L+ P+  D       S   + +  A+ F  D   GHG+H A T AGST++SP +L +C  G + +GC GGC+  S      ++   DI+ LCP F C       + CL DD A+TLS + G+A GAK+A  DV   G   + +LAGNGLWE     G  +HSNS+G    C +    +L+D YMY+N EHLLLF+AGN G  +D  TCT+ SP +AKNVL VG++SSG TR ++T  DG      D+  S ID VS FS  G TED RIKPE+VAPGD + S S +G D  +C+L    G+S +  V +  AAM+RQYF++  FY+ D+E+RG C        F C  F+  A TVKAMLINSAN+MGG +EPD  RGFGR+HLE G+PLGG GD+ LFVADSS+  V       + F + D DAG E RATL W DP A+++S +QL++DLDL V +P G  Y MW  N  DTSNV ERVIV  E  VA+ + T  W V V        ++  +SLVVTG     I  N A V                  A  ++ RG    + F  AGV + +A LAA+
Sbjct:  288 GLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDS--TPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGD------GSSSGMPFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSG-----DQCLPDDTADTLSEHGGMAQGAKIAAIDVFY-GDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDEL-STIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQG-----FTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNV-DPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAE-VVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISPPIEGNSAVVXXXXXXXXXXXXXXATGAAVTMYGRGAGAGSGFVLAGVAMLAAALAAA 1002          

BLAST of mRNA_H-elongata_contig8179.15847.1 vs. uniprot
Match: A0A6H5L2G3_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L2G3_9PHAE)

HSP 1 Score: 515 bits (1326), Expect = 3.020e-164
Identity = 326/716 (45.53%), Postives = 426/716 (59.50%), Query Frame = 2

Query:   65 MACFMSLVSYLASRAEVLRVAPYHRKTILNAVAKAISHSATTDTSETTLTAAGLDGTGEVIQIIDSGLDQDSCFFRDSDGQNVEGGYYFDEIA-----IISSTEVFSWFDS---GGSFPVDLDRRKIVQYISLVKPEDEDETYIFTSSGQQIHWYDAEGFAVDTAGGHGSHTAGTAAGSTVTSPVQLGTCGGDDE-LGCLGGCLNTSYVEQLLSNQYVDIDVLCPKFDCDTLGTDFETCLSDDVAETLSANEGVAPGAKLAIFDVSVDGIVIWAELAGNGLWEASNGTGSVVHSNSWGGVSDCTVDDASILFDTYMYKNPEHLLLFAAGNDGDPSDT-TCTVGSPAVAKNVLAVGASSSGETRWTSTGDDGEQILTIDQTWSDIDTVSFFSSRGPTEDDRIKPELVAPGDRVLSVSSSGDDGETCKLELQVGSSMAVPVVAAGAAMIRQYFKNDDFYTTDLETRGLCGGNSTYSTFVCSPFNASAATVKAMLINSANLMGGSSEPDGSRGFGRVHLEGGLPLGGLGDVGLFVADSSNTNVTQGAPDYYTFELLDGDAG-EFRATLVWIDPPATTISKNQLIHDLDLTVSSPSGQTYTMWSSNKADTSNVIERVIVSVEDFVAEDNGT--WTVSVTANGLMT-ESQPYSLVVTGPFGNGIIVNDA--------------------SVAAPLFHRGVVFLTSFFCAGVGLASALLAAS 2110
            MAC+M LVS+ ASR EVL+V  ++RK  LNA A+A   SA+ D+  T LT AGLDG+GEVIQIID+G+++ +CF    DG  VE GYYFD +A       SS+E  S F +   GG F  D DRRK++QYI L+ P+  D       S   + +  A+ F  D   GHG+HTA T AGST++SP +L +C  + E +GC GGC+  S      ++   DI+ LCP F C       + CL DD A TLS + G+A GAK+A  D+   G   + +LAGNGLWE     G  +HSNS+G    C +    +L+D YMY+N EHLLLF+AGN G  +D  TCT+ SP +AKNVL VG++SSG TR ++T  DG      D+  S ID VS FS  G TED RIKPE+VAPGD + S S +G +  +C+L    G+S +  V +  AAM+RQYF++  FY  D+E+RG C        F C  F+  A TVKAMLINSAN+MGG +EPD  RGFGR+HLE G+PLGG GD+ LFVADSS+  V+      + F + D DAG E RATL W DP A+++S +QL++DLDL V +P G  Y MW  N  DTSNV ERVIV  E  VA+ + T  W V V        ++  +SLVVTG     I  + A                      AA ++ RG    + F  AGV + +A LAA+
Sbjct:    1 MACYMGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDS--TPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPSFDSSSESRSMFKAEFTGGDFFYDSDRRKVIQYIDLINPDTGD------GSSSGMPFVSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCADNGEVIGCAGGCIIESSTGSTEADYGEDINRLCPAFGCSG-----DQCLPDDTANTLSEHGGMAQGAKIAAIDIFY-GDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDEL-STIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGNS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQG-----FTCDSFSPMATTVKAMLINSANVMGGGNEPDSERGFGRIHLEAGMPLGGAGDMVLFVADSSDHEVSSNGKLKFNFNV-DPDAGLELRATLSWSDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAE-VVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISPPIEGSSAXXXXXXXXXXXXXXXXXXXXGAAATMYGRGAGAGSGFVLAGVAMLAAALAAA 693