mRNA_H-elongata_contig6840.14472.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig6840.14472.1
Unique NamemRNA_H-elongata_contig6840.14472.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig6840.14472.1 vs. uniprot
Match: A0A6H5L457_9PHAE (zf-Tim10_DDP domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5L457_9PHAE)

HSP 1 Score: 142 bits (357), Expect = 1.440e-39
Identity = 71/93 (76.34%), Postives = 83/93 (89.25%), Query Frame = 1 
Query:    1 MTPAQQQQFMQYLETQQRKDSLAMYNNLVFKCFEDCSRSFRSRRLDDSETKCINVCAEKFIKLTGRVARRFQDLQQQKAKEEQAAATAAGLQR 279
            MTP QQQ F+Q+LE+QQRKDSLAMYNNLVF+CF++CS+SFRS+RLDD ETKCINVCAEK+IKLT RVA RFQD+QQQKAK+E     AAG+QR
Sbjct:    1 MTPVQQQDFLQHLESQQRKDSLAMYNNLVFRCFDECSKSFRSKRLDDGETKCINVCAEKYIKLTSRVALRFQDIQQQKAKDE-----AAGVQR 88
[Close]
BLAST of mRNA_H-elongata_contig6840.14472.1 vs. uniprot
Match: A0A024TE29_9STRA (zf-Tim10_DDP domain-containing protein n=4 Tax=Aphanomyces TaxID=100860 RepID=A0A024TE29_9STRA)

HSP 1 Score: 79.7 bits (195), Expect = 1.570e-15
Identity = 37/76 (48.68%), Postives = 55/76 (72.37%), Query Frame = 1 
Query:    1 MTPAQQQQFMQYLETQQRKDSLAMYNNLVFKCFEDCSRSFRSRRLDDSETKCINVCAEKFIKLTGRVARRFQDLQQ 228
            + P QQQ+ M++ E +Q ++SL +YN +V  CF +C +SFRS++L+D E  C+N+CAEKF+K T RV  RF + QQ
Sbjct:    7 LPPHQQQELMKHFEQKQLEESLMLYNRVVATCFNECVQSFRSKKLEDKEENCMNLCAEKFLKHTQRVGVRFAEAQQ 82
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BLAST of mRNA_H-elongata_contig6840.14472.1 vs. uniprot
Match: W4FPA0_9STRA (zf-Tim10_DDP domain-containing protein n=2 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FPA0_9STRA)

HSP 1 Score: 80.1 bits (196), Expect = 2.520e-13
Identity = 37/76 (48.68%), Postives = 55/76 (72.37%), Query Frame = 1 
Query:    1 MTPAQQQQFMQYLETQQRKDSLAMYNNLVFKCFEDCSRSFRSRRLDDSETKCINVCAEKFIKLTGRVARRFQDLQQ 228
            + P QQQ+ M++ E +Q ++SL +YN +V  CF +C +SFRS++L+D E  C+N+CAEKF+K T RV  RF + QQ
Sbjct:    7 LPPHQQQELMKHFEQKQLEESLMLYNRVVATCFNECVQSFRSKKLEDKEVNCMNLCAEKFLKHTQRVGVRFAEAQQ 82
[Close]
BLAST of mRNA_H-elongata_contig6840.14472.1 vs. uniprot
Match: A0A7S3BME1_9EUKA (Hypothetical protein n=1 Tax=Haptolina ericina TaxID=156174 RepID=A0A7S3BME1_9EUKA)

HSP 1 Score: 73.6 bits (179), Expect = 5.720e-13
Identity = 35/62 (56.45%), Postives = 43/62 (69.35%), Query Frame = 1 
Query:   37 LETQQRKDSLAMYNNLVFKCFEDCSRSFRSRRLDDSETKCINVCAEKFIKLTGRVARRFQDL 222
            LE  Q KD+L M+N LV +CF +C   FRS+ L   E KCIN CAEKFIK +GRV +RF +L
Sbjct:   39 LEEMQMKDTLRMFNGLVERCFSECVNGFRSKALTAPEEKCINTCAEKFIKHSGRVGQRFGEL 100
[Close]
BLAST of mRNA_H-elongata_contig6840.14472.1 vs. uniprot
Match: A0A388M117_CHABU (zf-Tim10_DDP domain-containing protein n=1 Tax=Chara braunii TaxID=69332 RepID=A0A388M117_CHABU)

HSP 1 Score: 72.0 bits (175), Expect = 1.270e-12
Identity = 33/62 (53.23%), Postives = 44/62 (70.97%), Query Frame = 1 
Query:   37 LETQQRKDSLAMYNNLVFKCFEDCSRSFRSRRLDDSETKCINVCAEKFIKLTGRVARRFQDL 222
            LET Q +DSL +YN LV +CF +C  SFR + LD +E  C+  C EKF+K TGRV+ RFQ++
Sbjct:   21 LETVQMRDSLRLYNTLVERCFNNCVDSFRRKTLDKTEETCVKRCVEKFLKHTGRVSLRFQEI 82
[Close]
BLAST of mRNA_H-elongata_contig6840.14472.1 vs. uniprot
Match: A0A1E5RJ12_HANUV (Mitochondrial import inner membrane translocase subunit TIM9 n=3 Tax=Hanseniaspora TaxID=29832 RepID=A0A1E5RJ12_HANUV)

HSP 1 Score: 70.9 bits (172), Expect = 3.090e-12
Identity = 33/68 (48.53%), Postives = 48/68 (70.59%), Query Frame = 1 
Query:   16 QQQFMQYLETQQRKDSLAMYNNLVFKCFEDCSRSFRSRRLDDSETKCINVCAEKFIKLTGRVARRFQD 219
            QQ+F + +E +Q KD + +Y+NLV KCF DC   F S +L++ E  CI+ CAEKF+K + RV +RFQ+
Sbjct:    9 QQEFQKIVEQKQMKDFMRLYSNLVEKCFSDCVEDFTSSKLNNRELTCISKCAEKFLKHSERVGKRFQE 76
[Close]
BLAST of mRNA_H-elongata_contig6840.14472.1 vs. uniprot
Match: A0A7S3RAD0_DUNTE (Hypothetical protein n=1 Tax=Dunaliella tertiolecta TaxID=3047 RepID=A0A7S3RAD0_DUNTE)

HSP 1 Score: 68.6 bits (166), Expect = 4.440e-11
Identity = 31/61 (50.82%), Postives = 40/61 (65.57%), Query Frame = 1 
Query:   37 LETQQRKDSLAMYNNLVFKCFEDCSRSFRSRRLDDSETKCINVCAEKFIKLTGRVARRFQD 219
            LE  Q +DSL MYNNLV +CF +CS   RS+ L   E +C+  C EKF+ +TGRV  RF +
Sbjct:   27 LEHMQVRDSLKMYNNLVERCFRECSEDMRSKALSSKEEQCVVKCCEKFMNVTGRVGMRFSE 87
[Close]
BLAST of mRNA_H-elongata_contig6840.14472.1 vs. uniprot
Match: A0A7S2CLT1_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2CLT1_9STRA)

HSP 1 Score: 67.4 bits (163), Expect = 1.180e-10
Identity = 32/68 (47.06%), Postives = 43/68 (63.24%), Query Frame = 1 
Query:   22 QFMQYLETQQRKDSLAMYNNLVFKCFEDCSRSFRSRRLDDSETKCINVCAEKFIKLTGRVARRFQDLQ 225
            + +Q +E  Q  +S   YN +V  CF DC ++FRS  L D E+KC+N CAEKFIKL  R   R+Q+ Q
Sbjct:   34 ELLQEIEKMQMHNSSKTYNQMVEHCFNDCIKTFRSSTLSDQESKCVNSCAEKFIKLGRRAGYRYQEHQ 101
[Close]
BLAST of mRNA_H-elongata_contig6840.14472.1 vs. uniprot
Match: A0A836CES0_9STRA (zf-Tim10_DDP domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CES0_9STRA)

HSP 1 Score: 65.1 bits (157), Expect = 5.630e-10
Identity = 30/75 (40.00%), Postives = 48/75 (64.00%), Query Frame = 1 
Query:    1 MTPAQQQQFMQYLETQQRKDSLAMYNNLVFKCFEDCSRSFRSRRLDDSETKCINVCAEKFIKLTGRVARRFQDLQ 225
            +T A+Q + M ++E +Q K+S+  YN   +KCF  C  +F++++LD +ET CI  C  KF+KL+ R  +R  D Q
Sbjct:    5 LTEAEQAKLMGFMEFEQVKNSMKAYNQTAYKCFGQCVTTFKTKKLDATETACIENCTAKFLKLSQRTGQRMGDYQ 79
[Close]
BLAST of mRNA_H-elongata_contig6840.14472.1 vs. uniprot
Match: M1VL90_CYAM1 (Mitochondrial intermembrane space complex subunit Tim9 n=1 Tax=Cyanidioschyzon merolae (strain NIES-3377 / 10D) TaxID=280699 RepID=M1VL90_CYAM1)

HSP 1 Score: 62.8 bits (151), Expect = 5.460e-9
Identity = 28/62 (45.16%), Postives = 40/62 (64.52%), Query Frame = 1 
Query:   28 MQYLETQQRKDSLAMYNNLVFKCFEDCSRSFRSRRLDDSETKCINVCAEKFIKLTGRVARRF 213
            M  L+  Q +DS+ +YN LV+ CF +C  SFRS +L+  E  C+  CA+KF+KL  R  +RF
Sbjct:   17 MAVLQELQIQDSMRLYNELVYLCFNNCVDSFRSSKLEAKEEACVTKCAQKFLKLAARAGQRF 78
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig6840.14472.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 22
Match NameE-valueIdentityDescription
A0A6H5L457_9PHAE1.440e-3976.34zf-Tim10_DDP domain-containing protein n=2 Tax=Ect... [more]
A0A024TE29_9STRA1.570e-1548.68zf-Tim10_DDP domain-containing protein n=4 Tax=Aph... [more]
W4FPA0_9STRA2.520e-1348.68zf-Tim10_DDP domain-containing protein n=2 Tax=Aph... [more]
A0A7S3BME1_9EUKA5.720e-1356.45Hypothetical protein n=1 Tax=Haptolina ericina Tax... [more]
A0A388M117_CHABU1.270e-1253.23zf-Tim10_DDP domain-containing protein n=1 Tax=Cha... [more]
A0A1E5RJ12_HANUV3.090e-1248.53Mitochondrial import inner membrane translocase su... [more]
A0A7S3RAD0_DUNTE4.440e-1150.82Hypothetical protein n=1 Tax=Dunaliella tertiolect... [more]
A0A7S2CLT1_9STRA1.180e-1047.06Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A836CES0_9STRA5.630e-1040.00zf-Tim10_DDP domain-containing protein n=1 Tax=Tri... [more]
M1VL90_CYAM15.460e-945.16Mitochondrial intermembrane space complex subunit ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig6840contigH-elongata_contig6840:1124..3114 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score144.8
Seed ortholog evalue2e-32
Seed eggNOG ortholog2880.D7G6I8
Preferred nameTIMM9
KEGG koko:K17777,ko:K17779
KEGG TC3.A.8.1
GOsGO:0000003,GO:0001666,GO:0003008,GO:0003674,GO:0005215,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005744,GO:0005758,GO:0006605,GO:0006626,GO:0006810,GO:0006839,GO:0006886,GO:0006950,GO:0006996,GO:0007005,GO:0007006,GO:0007007,GO:0007155,GO:0007160,GO:0007600,GO:0007605,GO:0008104,GO:0008150,GO:0008270,GO:0008320,GO:0008565,GO:0009628,GO:0009987,GO:0015031,GO:0015399,GO:0015405,GO:0015450,GO:0015833,GO:0016020,GO:0016021,GO:0016043,GO:0017038,GO:0019866,GO:0022610,GO:0022804,GO:0022857,GO:0022884,GO:0031090,GO:0031224,GO:0031589,GO:0031966,GO:0031967,GO:0031970,GO:0031974,GO:0031975,GO:0032501,GO:0032991,GO:0033036,GO:0033365,GO:0033554,GO:0034613,GO:0036293,GO:0036294,GO:0040008,GO:0040014,GO:0042221,GO:0042719,GO:0042721,GO:0042802,GO:0042803,GO:0042886,GO:0042887,GO:0043167,GO:0043169,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044422,GO:0044424,GO:0044425,GO:0044429,GO:0044444,GO:0044446,GO:0044455,GO:0044464,GO:0044743,GO:0045039,GO:0045184,GO:0046872,GO:0046907,GO:0046914,GO:0046983,GO:0048638,GO:0050789,GO:0050793,GO:0050877,GO:0050896,GO:0050954,GO:0051082,GO:0051087,GO:0051179,GO:0051234,GO:0051239,GO:0051641,GO:0051649,GO:0051716,GO:0055085,GO:0061024,GO:0065002,GO:0065007,GO:0070013,GO:0070482,GO:0070585,GO:0070727,GO:0070887,GO:0071453,GO:0071456,GO:0071702,GO:0071705,GO:0071806,GO:0071840,GO:0072321,GO:0072594,GO:0072655,GO:0072657,GO:0090150,GO:0090151,GO:0098796,GO:0098798,GO:0098800,GO:1904680,GO:1990351,GO:1990542
EggNOG free text desc.chaperone-mediated protein transport
EggNOG OGsKOG3479@1,KOG3479@2759
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko02000,ko03029
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionMitochondrial protein import TIM9.10 complex subunit, Tim9 homolog
Ec32 orthologEc-05_006340.1
Exons3
Model size822
Cds size282
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig6840.14472.1prot_H-elongata_contig6840.14472.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig6840 1124..2574 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931077.9607358-CDS-H-elongata_contig6840:1123..11741622931077.9607358-CDS-H-elongata_contig6840:1123..1174Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig6840 1124..1174 +
1691679688.9548597-CDS-H-elongata_contig6840:1123..11741691679688.9548597-CDS-H-elongata_contig6840:1123..1174Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig6840 1124..1174 +
1622931077.9743447-CDS-H-elongata_contig6840:1774..18371622931077.9743447-CDS-H-elongata_contig6840:1774..1837Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig6840 1775..1837 +
1691679688.9675395-CDS-H-elongata_contig6840:1774..18371691679688.9675395-CDS-H-elongata_contig6840:1774..1837Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig6840 1775..1837 +
1622931077.9943795-CDS-H-elongata_contig6840:2406..25741622931077.9943795-CDS-H-elongata_contig6840:2406..2574Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig6840 2407..2574 +
1691679688.9778495-CDS-H-elongata_contig6840:2406..25741691679688.9778495-CDS-H-elongata_contig6840:2406..2574Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig6840 2407..2574 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931078.013596-UTR-H-elongata_contig6840:2574..31141622931078.013596-UTR-H-elongata_contig6840:2574..3114Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig6840 2575..3114 +
1691679688.9872139-UTR-H-elongata_contig6840:2574..31141691679688.9872139-UTR-H-elongata_contig6840:2574..3114Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig6840 2575..3114 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig6840.14472.1

>prot_H-elongata_contig6840.14472.1 ID=prot_H-elongata_contig6840.14472.1|Name=mRNA_H-elongata_contig6840.14472.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=94bp
MTPAQQQQFMQYLETQQRKDSLAMYNNLVFKCFEDCSRSFRSRRLDDSET
KCINVCAEKFIKLTGRVARRFQDLQQQKAKEEQAAATAAGLQR*
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mRNA from alignment at H-elongata_contig6840:1124..3114+

Legend: polypeptideCDSUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig6840.14472.1 ID=mRNA_H-elongata_contig6840.14472.1|Name=mRNA_H-elongata_contig6840.14472.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1991bp|location=Sequence derived from alignment at H-elongata_contig6840:1124..3114+ (Himanthalia elongata Himel1 dioecious)
ATGACCCCCGCGCAGCAGCAGCAGTTCATGCAGTACCTCGAGACGCAGCA
GGTGATTTCACGAGACAGAAAGGAGGGGCGGGCGAATTACGTGATATTAT
TGATGCTGGGAATCGAGGAACTTATCCGATCCACACGGCGTCGTGGCATC
GCCGCCCATACGGGAGGAGGAGGCTTACTTGCTGCATTCTATTGCTGGAC
CGCGAAATAAATCTGTTGCGGTCTGCCCTTCGCCCTCGAATGAGAATTTA
CTGTTATGCCGTTTAACCTTTTCTCTTCCTCCGTGGGCAGCGGTCATCCG
TCATCAGTCATCAAGGCGAGGCCTTGCGTCGGAACACGTGTTCATCGGCG
ACCGAACTCTGCCTGCCTGTGGCCTTCTTTATTCAGACGCCGCGGAGTTT
ATCCCCCTTGCCAAGCAGTTAAAATATATACTACAACTATCAATAGAAGA
AAAAGCTTTCGCAATCTGTCGTGGGGAAGGTAAAAATATCTAATGGCGTT
GTATTTAAAACCAATCTGTGCCGTTATTATGGCGGTGGCCGCGAAGGACA
ACTCATTCATCCAGTCAACAAACTACCGTCTACCCCGCCTCCCTGCTCGC
CACCCCGACCTCGCGGAATGCCTACTTCGGAAACCCGTGTTCTTGGTGCA
GAGGAAGGACTCGCTCGCGATGTACAACAACCTCGTTTTCAAGTGTTTCG
AAGATTGCTCAAGGGTGAGGCGGGAAGAGGCGAAGGCAGCGATCGTCCCT
GTGTTTTGATGTGACCGTAGTTACACCGAGCACAAGACGGAGTGGGAGAG
AAGCAGCAGCGGTATGACGGGTTGCGGTATGGAGTCCTGCCTTTATGTGT
GCGAAGGATATCTTTTTTACTCCAGTACCATAAAATCTTGGCGTTTTTGT
TTCAGGCGTTTCAGGAAAACGAGCGCGCTATCGCCAATAATGCCCAACAT
TTATTTCTGGGGAAAGGCGTCCGAGTTGTTGGGTGTCTCTGATATTATTA
GTGTTGTTTTTGTGAAATTATTAGTGCGCGCTCTACCACTGCGAGACACC
AGGTAGATGGTTTTGAGCTCGGTTTCAACCTCCAATGGCTCACGACTCAC
GAATGCCGCCCGTCAAAACCTTGCGCGGATGGATGACCTCATCGAACGTG
TATTTTCCTAGAAGTGACAAGGACGTGACGTCTTAGCTTTCCATTCTCCT
GCGTAAAGCTGACTCGCTTTTCTACCGACCGTCCCTGCCACCCCCCGCCC
CGCCCCGCCCCGGCCGGACTCGAATCGATCCAGAGCTTCCGAAGCAGGCG
GCTAGACGATAGCGAGACGAAGTGCATCAACGTCTGCGCGGAGAAGTTCA
TCAAGCTTACGGGCAGGGTGGCGCGACGGTTCCAGGATCTTCAACAGCAG
AAGGCCAAGGAGGAGCAGGCTGCAGCGACGGCGGCCGGGCTACAACGCTG
ATCTATATTATATTAAATATATGTAATTTTCTACTTAACTTTTTGTTCTT
ACTTTTTTGCTGATGAAGTGGGAACTGTTCAACGTGCAAACTGCCATATG
CTTCGAGCTGCAATTAACTGGTATATTGGATTGGTTTGCATCGGAGGGCG
TAGCGGATCGCACTTGATAAAGAGGGAGCCAATTTTTGCAAGTCTTTCGC
TTTAAGGGCGGATACTGACAGGATGCAGCTAAGGCGGCTGCAACAACTGA
ACAGAAGTAATGTTTTCTACCTGGTAGGTAATAGGTACCACGCCGGGGAT
AAAATGATGTCGCCCCTTCCGCGAATAGCCTCGAAAAAAGTGTATATATA
TTTATACCACTCTACGGAGCGACATATTGACAAGAGCTGTGGATGAAACT
CCCTACATTTCAAAGTGCCCAAACGCCATTTTCGACCCAAAATTGTAGAG
GACGACATGTTCCCTTTTCCGGGTAAGGAAGTTTGGTACCCCCCCCCCCT
CCGAATGATATTTGGAGGTTTGTGCAAAAATGGCTTCTCGG
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Coding sequence (CDS) from alignment at H-elongata_contig6840:1124..3114+

>mRNA_H-elongata_contig6840.14472.1 ID=mRNA_H-elongata_contig6840.14472.1|Name=mRNA_H-elongata_contig6840.14472.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=564bp|location=Sequence derived from alignment at H-elongata_contig6840:1124..3114+ (Himanthalia elongata Himel1 dioecious)
ATGACCCCCGCGCAGCAGCAGCAGTTCATGCAGTACCTCGAGACGCAGCA
GATGACCCCCGCGCAGCAGCAGCAGTTCATGCAGTACCTCGAGACGCAGC
AGAGGAAGGACTCGCTCGCGATGTACAACAACCTCGTTTTCAAGTGTTTC
GAAGATTGCTCAAGGAGGAAGGACTCGCTCGCGATGTACAACAACCTCGT
TTTCAAGTGTTTCGAAGATTGCTCAAGGAGCTTCCGAAGCAGGCGGCTAG
ACGATAGCGAGACGAAGTGCATCAACGTCTGCGCGGAGAAGTTCATCAAG
CTTACGGGCAGGGTGGCGCGACGGTTCCAGGATCTTCAACAGCAGAAGGC
CAAGGAGGAGCAGGCTGCAGCGACGGCGGCCGGGCTACAACGCTGAAGCT
TCCGAAGCAGGCGGCTAGACGATAGCGAGACGAAGTGCATCAACGTCTGC
GCGGAGAAGTTCATCAAGCTTACGGGCAGGGTGGCGCGACGGTTCCAGGA
TCTTCAACAGCAGAAGGCCAAGGAGGAGCAGGCTGCAGCGACGGCGGCCG
GGCTACAACGCTGA
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