mRNA_H-elongata_contig6666.14268.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig6666.14268.1
Unique NamemRNA_H-elongata_contig6666.14268.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig6666.14268.1 vs. uniprot
Match: D7FLD9_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FLD9_ECTSI)

HSP 1 Score: 178 bits (452), Expect = 1.880e-53
Identity = 80/109 (73.39%), Postives = 96/109 (88.07%), Query Frame = 1 
Query:    1 MATTPLISIETEHNSIIHDAQLDYYGKKLATASSDHNITIFEIVGENQHNQIERLTGHEGPVWQVAWAHPKFGVILASCSYDRSIIIYRESPPGTWSRVHKHDDLHTSS 327
            MATT L +I+T H+  IHDAQLDYYGKKLATASSD  I IFE+VG++ HNQ++ L+GH+GPVWQV WAHPKFGV+LASCSYD+S+II+RE+PPGTWS VHKH+ LHTSS
Sbjct:    1 MATTALQTIDTHHSGNIHDAQLDYYGKKLATASSDCKINIFEVVGDSHHNQLDSLSGHDGPVWQVGWAHPKFGVLLASCSYDKSVIIHRETPPGTWSPVHKHE-LHTSS 108
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BLAST of mRNA_H-elongata_contig6666.14268.1 vs. uniprot
Match: A0A836CBZ9_9STRA (WD40-repeat-containing domain protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CBZ9_9STRA)

HSP 1 Score: 150 bits (380), Expect = 1.390e-42
Identity = 68/102 (66.67%), Postives = 83/102 (81.37%), Query Frame = 1 
Query:    1 MATTPLISIETEHNSIIHDAQLDYYGKKLATASSDHNITIFEIVGENQHNQIERLTGHEGPVWQVAWAHPKFGVILASCSYDRSIIIYRESPPGTWSRVHKH 306
            M+ + L++IET+HN +IHDAQLDYY +KLATASSD  I IF+I GE   N+   LTGHEGPVWQVAWAHP+FGV+LASCSYD S++I+ ESPPG W+ VH H
Sbjct:    1 MSVSTLVNIETQHNEMIHDAQLDYYSRKLATASSDRTIEIFDINGEVT-NRSAVLTGHEGPVWQVAWAHPRFGVLLASCSYDGSVLIHTESPPGVWTTVHAH 101
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BLAST of mRNA_H-elongata_contig6666.14268.1 vs. uniprot
Match: A0A7S3UQX2_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UQX2_HETAK)

HSP 1 Score: 145 bits (366), Expect = 2.350e-42
Identity = 63/102 (61.76%), Postives = 81/102 (79.41%), Query Frame = 1 
Query:    1 MATTPLISIETEHNSIIHDAQLDYYGKKLATASSDHNITIFEIVGENQHNQIERLTGHEGPVWQVAWAHPKFGVILASCSYDRSIIIYRESPPGTWSRVHKH 306
            MA++  + IET H  +IHDAQ DYY KK+ATASSD  I I++I  ++ +N+   LTGHEGPVWQVAWAHPK+GV+LASCSYD  +I++RESPPG WS++H H
Sbjct:    1 MASSAPVMIETSHEDMIHDAQFDYYAKKVATASSDRTIQIYDIT-DDVYNKSAVLTGHEGPVWQVAWAHPKYGVLLASCSYDGQVILHRESPPGVWSQIHSH 101
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BLAST of mRNA_H-elongata_contig6666.14268.1 vs. uniprot
Match: A0A7S2FN42_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2FN42_9STRA)

HSP 1 Score: 145 bits (366), Expect = 1.490e-40
Identity = 63/106 (59.43%), Postives = 84/106 (79.25%), Query Frame = 1 
Query:   10 TPLISIETEHNSIIHDAQLDYYGKKLATASSDHNITIFEIVGENQHNQIERLTGHEGPVWQVAWAHPKFGVILASCSYDRSIIIYRESPPGTWSRVHKHDDLHTSS 327
            TPL++ +T+H+ +IHDAQLDYY +KLATASSD +I ++++ GE  +     L GHEGPVWQ AWAHPKFGV+LASCSYD  ++I+RESPPG W+ +H+H  +H SS
Sbjct:    3 TPLVTFDTQHDDMIHDAQLDYYSRKLATASSDRSIKVWDVQGE-VYTLAATLNGHEGPVWQCAWAHPKFGVLLASCSYDGKVLIHRESPPGVWTPIHEHT-MHDSS 106
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BLAST of mRNA_H-elongata_contig6666.14268.1 vs. uniprot
Match: A0A7S0FIQ5_9STRA (Hypothetical protein (Fragment) n=1 Tax=Minutocellus polymorphus TaxID=265543 RepID=A0A7S0FIQ5_9STRA)

HSP 1 Score: 142 bits (357), Expect = 1.670e-40
Identity = 59/105 (56.19%), Postives = 83/105 (79.05%), Query Frame = 1 
Query:   19 ISIETEHNSIIHDAQLDYYGKKLATASSDHNITIFEIVGENQHNQIERLTGHEGPVWQVAWAHPKFGVILASCSYDRSIIIYRESPPGTWSRVHKHDDLHTSSVN 333
            I I+T+H+ ++HD+QLDYYG KLATASSD  + I+++ G N +     L GHEGPVW+++W+HPKFGV+LASCS+D S++++RESPPG+W+ +H    LH SSVN
Sbjct:    6 IVIDTQHDDMVHDSQLDYYGTKLATASSDRTVKIYDVSG-NTYAHTATLQGHEGPVWELSWSHPKFGVVLASCSFDGSVLVHRESPPGSWTLIHAARALHDSSVN 109
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BLAST of mRNA_H-elongata_contig6666.14268.1 vs. uniprot
Match: K3XB55_GLOUD (Uncharacterized protein n=2 Tax=Pythiaceae TaxID=4782 RepID=K3XB55_GLOUD)

HSP 1 Score: 142 bits (359), Expect = 1.450e-39
Identity = 60/100 (60.00%), Postives = 79/100 (79.00%), Query Frame = 1 
Query:    7 TTPLISIETEHNSIIHDAQLDYYGKKLATASSDHNITIFEIVGENQHNQIERLTGHEGPVWQVAWAHPKFGVILASCSYDRSIIIYRESPPGTWSRVHKH 306
            T P++SI+T+H  +IHDAQLDYYGK+LAT SSD  + ++++ GE Q+N+ + LTGHEGPVWQVAWAHPKFG +LASCSYD  +IIYRE     W++ + H
Sbjct:    2 TAPILSIDTQHEDMIHDAQLDYYGKRLATCSSDRTVKVYDVTGEVQNNE-QLLTGHEGPVWQVAWAHPKFGALLASCSYDGKVIIYREQSLNQWAQTYVH 100
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BLAST of mRNA_H-elongata_contig6666.14268.1 vs. uniprot
Match: H3GAW1_PHYRM (Uncharacterized protein n=7 Tax=Peronosporaceae TaxID=4777 RepID=H3GAW1_PHYRM)

HSP 1 Score: 140 bits (354), Expect = 8.270e-39
Identity = 59/100 (59.00%), Postives = 80/100 (80.00%), Query Frame = 1 
Query:    7 TTPLISIETEHNSIIHDAQLDYYGKKLATASSDHNITIFEIVGENQHNQIERLTGHEGPVWQVAWAHPKFGVILASCSYDRSIIIYRESPPGTWSRVHKH 306
            + P++SI+T+H  +IHDAQLDYYGK+LAT SSD  I ++++ G+ Q+N+ + LTGHEGPVWQVAWAHPKFGV+LA+CSYD  +IIYRE     W++ + H
Sbjct:    2 SAPILSIDTQHEDMIHDAQLDYYGKRLATCSSDRTIKVYDVTGDVQNNE-QILTGHEGPVWQVAWAHPKFGVLLAACSYDGKVIIYREQSLNQWTQAYMH 100
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BLAST of mRNA_H-elongata_contig6666.14268.1 vs. uniprot
Match: D0NKZ1_PHYIT (Protein transport protein SEC13 n=16 Tax=Peronosporaceae TaxID=4777 RepID=D0NKZ1_PHYIT)

HSP 1 Score: 139 bits (350), Expect = 3.270e-38
Identity = 58/100 (58.00%), Postives = 80/100 (80.00%), Query Frame = 1 
Query:    7 TTPLISIETEHNSIIHDAQLDYYGKKLATASSDHNITIFEIVGENQHNQIERLTGHEGPVWQVAWAHPKFGVILASCSYDRSIIIYRESPPGTWSRVHKH 306
            + P++SI+T+H  +IHDAQLDYYGK+LAT SSD  I ++++ G+ Q+N+ + LTGHEGPVWQV+WAHPKFGV+LA+CSYD  +IIYRE     W++ + H
Sbjct:    2 SAPILSIDTQHEDMIHDAQLDYYGKRLATCSSDRTIKVYDVAGDVQNNE-QILTGHEGPVWQVSWAHPKFGVLLAACSYDGKVIIYREQSLNQWTQAYVH 100
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BLAST of mRNA_H-elongata_contig6666.14268.1 vs. uniprot
Match: A0A7S2MDV0_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2MDV0_9STRA)

HSP 1 Score: 139 bits (350), Expect = 5.470e-38
Identity = 60/102 (58.82%), Postives = 79/102 (77.45%), Query Frame = 1 
Query:    1 MATTPLISIETEHNSIIHDAQLDYYGKKLATASSDHNITIFEIVGENQHNQIERLTGHEGPVWQVAWAHPKFGVILASCSYDRSIIIYRESPPGTWSRVHKH 306
            MA + L++ +T+H+ +IHDAQLDYY +KLATASSD  I ++++ G+  +     LTGHEGPVWQVAWAHPKFGV+LASCSYD  ++I+RES  G WS +H H
Sbjct:   15 MAASALVTFDTQHDDMIHDAQLDYYSRKLATASSDRTIKVWDVQGK-VYTLAATLTGHEGPVWQVAWAHPKFGVLLASCSYDGKVLIHRESQTGVWSPIHSH 115
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BLAST of mRNA_H-elongata_contig6666.14268.1 vs. uniprot
Match: A0A7S2UZ04_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UZ04_9STRA)

HSP 1 Score: 137 bits (346), Expect = 2.290e-37
Identity = 59/102 (57.84%), Postives = 78/102 (76.47%), Query Frame = 1 
Query:    1 MATTPLISIETEHNSIIHDAQLDYYGKKLATASSDHNITIFEIVGENQHNQIERLTGHEGPVWQVAWAHPKFGVILASCSYDRSIIIYRESPPGTWSRVHKH 306
            MA    ++I+T+H  +IHDAQ DYY KK+AT SSD  I I+++ G+  +N+   LTGH+GPVWQVAWAHPKFGV+LASCSYD  +II+RESP   W+++H H
Sbjct:    1 MAADQPVTIDTQHEDMIHDAQFDYYAKKVATCSSDRTIQIYDVTGD-VYNKSAVLTGHDGPVWQVAWAHPKFGVLLASCSYDGQVIIHRESPQSCWTQIHVH 101
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig6666.14268.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FLD9_ECTSI1.880e-5373.39Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A836CBZ9_9STRA1.390e-4266.67WD40-repeat-containing domain protein n=1 Tax=Trib... [more]
A0A7S3UQX2_HETAK2.350e-4261.76Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A7S2FN42_9STRA1.490e-4059.43Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A7S0FIQ5_9STRA1.670e-4056.19Hypothetical protein (Fragment) n=1 Tax=Minutocell... [more]
K3XB55_GLOUD1.450e-3960.00Uncharacterized protein n=2 Tax=Pythiaceae TaxID=4... [more]
H3GAW1_PHYRM8.270e-3959.00Uncharacterized protein n=7 Tax=Peronosporaceae Ta... [more]
D0NKZ1_PHYIT3.270e-3858.00Protein transport protein SEC13 n=16 Tax=Peronospo... [more]
A0A7S2MDV0_9STRA5.470e-3858.82Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A7S2UZ04_9STRA2.290e-3757.84Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig6666contigH-elongata_contig6666:580..918 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score176.8
Seed ortholog evalue5.7e-42
Seed eggNOG ortholog2880.D7FLD9
Preferred nameSEC13
KEGG koko:K14004
KEGG Pathwayko03013,ko04141,ko04150,map03013,map04141,map04150
KEGG ModuleM00404,M00427
GOsGO:0000070,GO:0000278,GO:0000280,GO:0000323,GO:0000785,GO:0000819,GO:0002164,GO:0002165,GO:0002168,GO:0002376,GO:0002474,GO:0002478,GO:0002495,GO:0002504,GO:0003002,GO:0003674,GO:0003682,GO:0005198,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005635,GO:0005643,GO:0005654,GO:0005694,GO:0005700,GO:0005703,GO:0005730,GO:0005737,GO:0005764,GO:0005765,GO:0005773,GO:0005774,GO:0005783,GO:0005789,GO:0005798,GO:0005829,GO:0006323,GO:0006355,GO:0006357,GO:0006508,GO:0006511,GO:0006606,GO:0006807,GO:0006810,GO:0006886,GO:0006888,GO:0006900,GO:0006901,GO:0006903,GO:0006913,GO:0006950,GO:0006996,GO:0006997,GO:0007049,GO:0007059,GO:0007076,GO:0007275,GO:0007346,GO:0007389,GO:0007591,GO:0008104,GO:0008150,GO:0008152,GO:0008363,GO:0009056,GO:0009057,GO:0009266,GO:0009408,GO:0009628,GO:0009719,GO:0009725,GO:0009790,GO:0009791,GO:0009792,GO:0009889,GO:0009891,GO:0009892,GO:0009893,GO:0009966,GO:0009967,GO:0009987,GO:0010033,GO:0010243,GO:0010468,GO:0010498,GO:0010556,GO:0010557,GO:0010564,GO:0010604,GO:0010605,GO:0010628,GO:0010629,GO:0010646,GO:0010647,GO:0010973,GO:0012505,GO:0012506,GO:0012507,GO:0014070,GO:0015031,GO:0015833,GO:0016020,GO:0016043,GO:0016050,GO:0016192,GO:0017038,GO:0019219,GO:0019222,GO:0019538,GO:0019882,GO:0019884,GO:0019886,GO:0019941,GO:0022402,GO:0022607,GO:0023051,GO:0023056,GO:0030117,GO:0030120,GO:0030127,GO:0030133,GO:0030134,GO:0030135,GO:0030163,GO:0030261,GO:0030433,GO:0030658,GO:0030659,GO:0030660,GO:0030662,GO:0031080,GO:0031081,GO:0031090,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031334,GO:0031410,GO:0031503,GO:0031967,GO:0031974,GO:0031975,GO:0031981,GO:0031982,GO:0031984,GO:0032006,GO:0032008,GO:0032465,GO:0032467,GO:0032501,GO:0032502,GO:0032870,GO:0032954,GO:0032955,GO:0032991,GO:0033036,GO:0033365,GO:0033554,GO:0033993,GO:0034504,GO:0034605,GO:0034613,GO:0034622,GO:0034629,GO:0034976,GO:0035017,GO:0035075,GO:0035077,GO:0035079,GO:0035293,GO:0035459,GO:0035859,GO:0036314,GO:0036315,GO:0036503,GO:0040003,GO:0042175,GO:0042221,GO:0042303,GO:0042335,GO:0042337,GO:0042802,GO:0042886,GO:0043085,GO:0043087,GO:0043161,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043254,GO:0043547,GO:0043632,GO:0043933,GO:0044085,GO:0044087,GO:0044089,GO:0044093,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044427,GO:0044428,GO:0044432,GO:0044433,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0045184,GO:0045787,GO:0045893,GO:0045931,GO:0045935,GO:0045944,GO:0046907,GO:0048002,GO:0048193,GO:0048194,GO:0048199,GO:0048207,GO:0048208,GO:0048285,GO:0048475,GO:0048518,GO:0048519,GO:0048522,GO:0048583,GO:0048584,GO:0048856,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051128,GO:0051130,GO:0051169,GO:0051170,GO:0051171,GO:0051173,GO:0051179,GO:0051234,GO:0051252,GO:0051254,GO:0051276,GO:0051302,GO:0051336,GO:0051345,GO:0051603,GO:0051640,GO:0051641,GO:0051648,GO:0051649,GO:0051650,GO:0051656,GO:0051664,GO:0051668,GO:0051716,GO:0051726,GO:0051781,GO:0060255,GO:0060260,GO:0060261,GO:0061024,GO:0061700,GO:0065003,GO:0065007,GO:0065009,GO:0070013,GO:0070727,GO:0070887,GO:0071103,GO:0071310,GO:0071390,GO:0071396,GO:0071407,GO:0071495,GO:0071702,GO:0071704,GO:0071705,GO:0071840,GO:0071944,GO:0072594,GO:0080090,GO:0090068,GO:0090110,GO:0090114,GO:0097305,GO:0097306,GO:0097708,GO:0098588,GO:0098687,GO:0098796,GO:0098805,GO:0098813,GO:0098827,GO:0098852,GO:0140014,GO:1901564,GO:1901565,GO:1901575,GO:1901654,GO:1901655,GO:1901698,GO:1901700,GO:1901701,GO:1901891,GO:1901893,GO:1902412,GO:1902531,GO:1902533,GO:1902680,GO:1903047,GO:1903432,GO:1903436,GO:1903438,GO:1903490,GO:1903506,GO:1903508,GO:1904263,GO:1990893,GO:2000112,GO:2000142,GO:2000144,GO:2001141
EggNOG free text desc.positive regulation of TORC1 signaling
EggNOG OGsKOG1332@1,KOG1332@2759
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03019,ko04131
Hectar predicted targeting categoryother localisation
Exons1
Model size339
Cds size339
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931056.3501182-CDS-H-elongata_contig6666:579..9181622931056.3501182-CDS-H-elongata_contig6666:579..918Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig6666 580..918 -
1691679680.0839508-CDS-H-elongata_contig6666:579..9181691679680.0839508-CDS-H-elongata_contig6666:579..918Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig6666 580..918 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig6666.14268.1prot_H-elongata_contig6666.14268.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig6666 580..918 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig6666.14268.1

>prot_H-elongata_contig6666.14268.1 ID=prot_H-elongata_contig6666.14268.1|Name=mRNA_H-elongata_contig6666.14268.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=113bp
MATTPLISIETEHNSIIHDAQLDYYGKKLATASSDHNITIFEIVGENQHN
QIERLTGHEGPVWQVAWAHPKFGVILASCSYDRSIIIYRESPPGTWSRVH
KHDDLHTSSVNR*
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mRNA from alignment at H-elongata_contig6666:580..918-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig6666.14268.1 ID=mRNA_H-elongata_contig6666.14268.1|Name=mRNA_H-elongata_contig6666.14268.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=339bp|location=Sequence derived from alignment at H-elongata_contig6666:580..918- (Himanthalia elongata Himel1 dioecious)
ATGGCCACCACTCCTCTTATCTCGATCGAGACAGAGCACAACAGCATCAT
CCACGACGCTCAGCTCGACTATTATGGCAAGAAGCTTGCAACGGCATCCA
GCGACCACAACATCACAATCTTCGAGATCGTCGGAGAAAACCAGCATAAC
CAGATCGAACGCCTCACGGGGCACGAGGGTCCCGTCTGGCAAGTTGCCTG
GGCCCACCCTAAGTTTGGAGTCATCCTGGCTTCTTGCTCGTACGACCGCT
CCATCATCATCTACCGTGAGTCACCGCCGGGAACTTGGAGTCGAGTTCAC
AAGCACGACGATCTCCACACCTCTAGCGTCAACAGGTAA
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Coding sequence (CDS) from alignment at H-elongata_contig6666:580..918-

>mRNA_H-elongata_contig6666.14268.1 ID=mRNA_H-elongata_contig6666.14268.1|Name=mRNA_H-elongata_contig6666.14268.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=678bp|location=Sequence derived from alignment at H-elongata_contig6666:580..918- (Himanthalia elongata Himel1 dioecious)
ATGGCCACCACTCCTCTTATCTCGATCGAGACAGAGCACAACAGCATCAT
CCACGACGCTCAGCTCGACTATTATGGCAAGAAGCTTGCAACGGCATCCA
GCGACCACAACATCACAATCTTCGAGATCGTCGGAGAAAACCAGCATAAC
CAGATCGAACGCCTCACGGGGCACGAGGGTCCCGTCTGGCAAGTTGCCTG
GGCCCACCCTAAGTTTGGAGTCATCCTGGCTTCTTGCTCGTACGACCGCT
CCATCATCATCTACCGTGAGTCACCGCCGGGAACTTGGAGTCGAGTTCAC
AAGCACGACGATCTCCACACCTCTAGCGTCAACAGGTAAATGGCCACCAC
TCCTCTTATCTCGATCGAGACAGAGCACAACAGCATCATCCACGACGCTC
AGCTCGACTATTATGGCAAGAAGCTTGCAACGGCATCCAGCGACCACAAC
ATCACAATCTTCGAGATCGTCGGAGAAAACCAGCATAACCAGATCGAACG
CCTCACGGGGCACGAGGGTCCCGTCTGGCAAGTTGCCTGGGCCCACCCTA
AGTTTGGAGTCATCCTGGCTTCTTGCTCGTACGACCGCTCCATCATCATC
TACCGTGAGTCACCGCCGGGAACTTGGAGTCGAGTTCACAAGCACGACGA
TCTCCACACCTCTAGCGTCAACAGGTAA
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