mRNA_H-elongata_contig57779.13247.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig57779.13247.1
Unique NamemRNA_H-elongata_contig57779.13247.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig57779.13247.1 vs. uniprot
Match: A0A6H5JZN2_9PHAE (Helicase ATP-binding domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JZN2_9PHAE)

HSP 1 Score: 141 bits (355), Expect = 4.340e-36
Identity = 73/134 (54.48%), Postives = 101/134 (75.37%), Query Frame = 1 
Query:   10 VEDSQEDK-DPELIGCPFYSNHVLAQQAQLVIVTYNYVLDPNIRSAVSLDLNDAIVVIDEAHNVEGVCRDAGSVELSVVDTANIAIDLCHLCQRTAYREDALVLLNFVLDLLRFTAWESGNLRDFEDETTFDDP 408
            V +++ED+ DP L+ CP+Y +  LA+QAQ+V++ YNY+LDP+IRSA+SL L  A+V++DEAHNVEGVCRDAGS+E+S++D   +A DLC   Q TA    A +LL FV+ LL FT+ ES  L  FE+ET F++P
Sbjct:  326 VSETKEDQADPHLLACPYYLSMALAKQAQIVLMPYNYILDPSIRSAMSLALEGAVVILDEAHNVEGVCRDAGSLEVSLLDMVAMASDLCDCSQLTACSLGANLLLEFVMKLLYFTSRESLTLEAFENETNFEEP 459
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BLAST of mRNA_H-elongata_contig57779.13247.1 vs. uniprot
Match: D8LEJ0_ECTSI (Helicase ATP-binding domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LEJ0_ECTSI)

HSP 1 Score: 124 bits (310), Expect = 4.780e-30
Identity = 63/111 (56.76%), Postives = 85/111 (76.58%), Query Frame = 1 
Query:   76 LAQQAQLVIVTYNYVLDPNIRSAVSLDLNDAIVVIDEAHNVEGVCRDAGSVELSVVDTANIAIDLCHLCQRTAYREDALVLLNFVLDLLRFTAWESGNLRDFEDETTFDDP 408
            LA+QAQ+V++ YNY+LDP++RSA+SL L  A+V++DEAHNVEGVCRDAGS+E+S++D   +A DLC   Q TA    A +LL FV+ LL FT+ ES  L  FE+ET F++P
Sbjct:    3 LAKQAQIVLMPYNYILDPSVRSAMSLALEGAVVILDEAHNVEGVCRDAGSLEVSLLDMVAMASDLCDCSQLTACSLGANLLLKFVMKLLYFTSKESRTLEAFENETDFEEP 113
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BLAST of mRNA_H-elongata_contig57779.13247.1 vs. uniprot
Match: R0MF66_NOSB1 (DNA helicase n=2 Tax=Nosema bombycis (strain CQ1 / CVCC 102059) TaxID=578461 RepID=R0MF66_NOSB1)

HSP 1 Score: 90.9 bits (224), Expect = 1.730e-18
Identity = 46/129 (35.66%), Postives = 81/129 (62.79%), Query Frame = 1 
Query:   49 GCPFYSNHVLAQQAQLVIVTYNYVLDPNIRSAVSLDLNDAIVVIDEAHNVEGVCRDAGSVELSVVDTANIAIDLCHLCQRTAYREDALVLLNFVLDLLRFTAWESGNLRDFEDETTFDDPLGLLEGEEV 435
            GCP++++  L ++A ++   YNY+LD N+R   +LDL++++V+IDEAHNV+ VCR +GS EL       I  DL    ++ A+ ED  + L  +LDL +     +  ++DF  +T+F+  + + +G+E+
Sbjct:  186 GCPYFASRKLNEKADIIFAPYNYLLDTNVRENTNLDLSNSVVIIDEAHNVDDVCRSSGSFELGNKLIEIICNDLISAIKKAAFFEDVKLDLLNILDLFKKFIEHAPKIKDFN-KTSFNGKMRIKKGKEI 313
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BLAST of mRNA_H-elongata_contig57779.13247.1 vs. uniprot
Match: A0A0Q3LFA9_BRADI (Helicase ATP-binding domain-containing protein n=1 Tax=Brachypodium distachyon TaxID=15368 RepID=A0A0Q3LFA9_BRADI)

HSP 1 Score: 86.3 bits (212), Expect = 7.600e-17
Identity = 45/127 (35.43%), Postives = 73/127 (57.48%), Query Frame = 1 
Query:   49 GCPFYSNHVLAQQAQLVIVTYNYVLDPNIRSAVSLDLNDAIVVIDEAHNVEGVCRDAGSVELSVVDTANIAIDLCHLCQRTAYREDALVLLNFVLDLLRFTAWESGNLRDFEDETTFDDPLGLLEGE 429
            GCP+++   +A+ AQLV   YNY++ P +R A+ +D++ +IV++DEAHN+E + RDAGSV++       +A +L +L    A  +    L   +  L+ +   + GNLRD E    F+ P     GE
Sbjct:  241 GCPYFAAQTMAETAQLVFCPYNYLISPIVRRAMDIDISGSIVILDEAHNIEDIARDAGSVDVDEESLTTLAGELANLATNEAVAKIYQPLHEVIEGLINWITDQVGNLRDNE----FEHPASFWTGE 363
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BLAST of mRNA_H-elongata_contig57779.13247.1 vs. uniprot
Match: A0A7S0B927_9DINO (Hypothetical protein (Fragment) n=1 Tax=Pyrodinium bahamense TaxID=73915 RepID=A0A7S0B927_9DINO)

HSP 1 Score: 84.0 bits (206), Expect = 2.450e-16
Identity = 41/76 (53.95%), Postives = 54/76 (71.05%), Query Frame = 1 
Query:   52 CPFYSNHVLAQQAQLVIVTYNYVLDPNIR-SAVSLDLNDAIVVIDEAHNVEGVCRDAGSVELSVVDTANIAIDLCH 276
            CP+Y+ H LAQQA LV   Y+YVLDP++R +A +LDL   +V++DEAHNVEGVCR+AGS E S+     +   L H
Sbjct:   71 CPYYTAHSLAQQADLVFCPYSYVLDPSVRRTAGALDLQGCVVILDEAHNVEGVCREAGSAEFSLDQMRAVIAGLQH 146
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BLAST of mRNA_H-elongata_contig57779.13247.1 vs. uniprot
Match: UPI0010FB8D30 (Fanconi anemia group J protein homolog n=1 Tax=Nylanderia fulva TaxID=613905 RepID=UPI0010FB8D30)

HSP 1 Score: 84.3 bits (207), Expect = 3.340e-16
Identity = 45/135 (33.33%), Postives = 80/135 (59.26%), Query Frame = 1 
Query:   49 GCPFYSNHVLAQQAQLVIVTYNYVLDPNIRSAVSLDLNDAIVVIDEAHNVEGVCRDAGSVELSVVDTANIAIDLCHLCQRTAYREDALVLLNF------VLDLLRFTAWESGNLRDFEDETTFDDPLGLLEGEEV 435
            GCP+Y++  LA +A ++   YNY++DP IR ++ +DL +AIV+IDEAHN+E  CR AGS+EL+     N  +D+      +A R+  + + +       +LD+++         R+F D+  FD  + + +G+++
Sbjct:  182 GCPYYASRSLADKADIIFAPYNYLIDPRIRESMGIDLKNAIVIIDEAHNIEDFCRSAGSLELN-----NRILDIIQNEVLSALRKGGISVFDIRGDITTILDVIKMFKEYGEEKREF-DKINFDTKIRVRKGKQI 310
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BLAST of mRNA_H-elongata_contig57779.13247.1 vs. uniprot
Match: A0A835GKE5_SPOEX (DNA helicase n=1 Tax=Spodoptera exigua TaxID=7107 RepID=A0A835GKE5_SPOEX)

HSP 1 Score: 84.3 bits (207), Expect = 3.390e-16
Identity = 35/85 (41.18%), Postives = 58/85 (68.24%), Query Frame = 1 
Query:   52 CPFYSNHVLAQQAQLVIVTYNYVLDPNIRSAVSLDLNDAIVVIDEAHNVEGVCRDAGSVELSVVDTANIAIDLCHLCQRTAYRED 306
            CP+Y    +A+ + +V   YNY++DP+IRS++S+DL  A+V+IDEAHN+EG+CRD  SV+++ +   N  ++L  + Q     +D
Sbjct:  220 CPYYGARAMAKTSHIVFCPYNYLIDPSIRSSLSIDLKGAVVIIDEAHNIEGICRDVASVDITQLQIQNAIVELEKVSQYRVANQD 304
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BLAST of mRNA_H-elongata_contig57779.13247.1 vs. uniprot
Match: A0A7N0UP03_KALFE (Helicase ATP-binding domain-containing protein n=2 Tax=Kalanchoe fedtschenkoi TaxID=63787 RepID=A0A7N0UP03_KALFE)

HSP 1 Score: 83.6 bits (205), Expect = 6.620e-16
Identity = 37/78 (47.44%), Postives = 56/78 (71.79%), Query Frame = 1 
Query:   49 GCPFYSNHVLAQQAQLVIVTYNYVLDPNIRSAVSLDLNDAIVVIDEAHNVEGVCRDAGSVELSVVDTANIAIDLCHLC 282
            GCP+Y++  +A  AQLV   YNY+++P IR+A+ +D+N A+V++DEAHN+E V RDAGSV+L       +  +L +LC
Sbjct:  254 GCPYYASRSMADDAQLVFCPYNYIINPVIRAAMEVDINGAVVILDEAHNIEDVARDAGSVDLEEDALDKLQTELQNLC 331
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BLAST of mRNA_H-elongata_contig57779.13247.1 vs. uniprot
Match: A0A2H1VWR6_SPOFR (DNA helicase n=1 Tax=Spodoptera frugiperda TaxID=7108 RepID=A0A2H1VWR6_SPOFR)

HSP 1 Score: 83.2 bits (204), Expect = 8.490e-16
Identity = 38/97 (39.18%), Postives = 63/97 (64.95%), Query Frame = 1 
Query:   52 CPFYSNHVLAQQAQLVIVTYNYVLDPNIRSAVSLDLNDAIVVIDEAHNVEGVCRDAGSVELSVVDTANIAIDLCHLCQRTAYREDALVLLNFVLDLL 342
            CP+Y    +A+ A +V   YNY++DP+IRS++S+DL  A+V+IDEAHN+EG+CRD  SV+++ +   N   +L  + Q     +D    ++ +L+ L
Sbjct:  134 CPYYGARAMAKTAHIVFCPYNYLIDPSIRSSLSIDLTGAVVIIDEAHNIEGICRDVASVDITQLQIQNAIKELETVSQYRFANQDVEYYIDGLLNTL 230
[Close]
BLAST of mRNA_H-elongata_contig57779.13247.1 vs. uniprot
Match: A0A2H1VVE1_SPOFR (DNA helicase (Fragment) n=1 Tax=Spodoptera frugiperda TaxID=7108 RepID=A0A2H1VVE1_SPOFR)

HSP 1 Score: 83.2 bits (204), Expect = 8.590e-16
Identity = 38/97 (39.18%), Postives = 63/97 (64.95%), Query Frame = 1 
Query:   52 CPFYSNHVLAQQAQLVIVTYNYVLDPNIRSAVSLDLNDAIVVIDEAHNVEGVCRDAGSVELSVVDTANIAIDLCHLCQRTAYREDALVLLNFVLDLL 342
            CP+Y    +A+ A +V   YNY++DP+IRS++S+DL  A+V+IDEAHN+EG+CRD  SV+++ +   N   +L  + Q     +D    ++ +L+ L
Sbjct:  206 CPYYGARAMAKTAHIVFCPYNYLIDPSIRSSLSIDLTGAVVIIDEAHNIEGICRDVASVDITQLQIQNAIKELETVSQYRFANQDVEYYIDGLLNTL 302
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig57779.13247.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JZN2_9PHAE4.340e-3654.48Helicase ATP-binding domain-containing protein n=1... [more]
D8LEJ0_ECTSI4.780e-3056.76Helicase ATP-binding domain-containing protein n=1... [more]
R0MF66_NOSB11.730e-1835.66DNA helicase n=2 Tax=Nosema bombycis (strain CQ1 /... [more]
A0A0Q3LFA9_BRADI7.600e-1735.43Helicase ATP-binding domain-containing protein n=1... [more]
A0A7S0B927_9DINO2.450e-1653.95Hypothetical protein (Fragment) n=1 Tax=Pyrodinium... [more]
UPI0010FB8D303.340e-1633.33Fanconi anemia group J protein homolog n=1 Tax=Nyl... [more]
A0A835GKE5_SPOEX3.390e-1641.18DNA helicase n=1 Tax=Spodoptera exigua TaxID=7107 ... [more]
A0A7N0UP03_KALFE6.620e-1647.44Helicase ATP-binding domain-containing protein n=2... [more]
A0A2H1VWR6_SPOFR8.490e-1639.18DNA helicase n=1 Tax=Spodoptera frugiperda TaxID=7... [more]
A0A2H1VVE1_SPOFR8.590e-1639.18DNA helicase (Fragment) n=1 Tax=Spodoptera frugipe... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig57779contigH-elongata_contig57779:284..2107 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score123.6
Seed ortholog evalue7.4e-26
Seed eggNOG ortholog2880.D8LEJ0
KEGG koko:K11136,ko:K15362
KEGG Pathwayko03440,ko03460,map03440,map03460
EggNOG free text desc.ATP-dependent DNA helicase activity
EggNOG OGsCOG1199@1,KOG1132@2759
EC3.6.4.12
COG Functional cat.KL
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03032,ko03400
Hectar predicted targeting categoryother localisation
Exons4
Model size436
Cds size435
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig57779.13247.1prot_H-elongata_contig57779.13247.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig57779 284..2107 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930937.9526596-CDS-H-elongata_contig57779:283..4191622930937.9526596-CDS-H-elongata_contig57779:283..419Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57779 284..419 -
1691679632.348566-CDS-H-elongata_contig57779:283..4191691679632.348566-CDS-H-elongata_contig57779:283..419Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57779 284..419 -
1622930937.9721591-CDS-H-elongata_contig57779:562..7381622930937.9721591-CDS-H-elongata_contig57779:562..738Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57779 563..738 -
1691679632.358389-CDS-H-elongata_contig57779:562..7381691679632.358389-CDS-H-elongata_contig57779:562..738Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57779 563..738 -
1622930937.987815-CDS-H-elongata_contig57779:1710..17611622930937.987815-CDS-H-elongata_contig57779:1710..1761Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57779 1711..1761 -
1691679632.3682919-CDS-H-elongata_contig57779:1710..17611691679632.3682919-CDS-H-elongata_contig57779:1710..1761Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57779 1711..1761 -
1622930938.0038848-CDS-H-elongata_contig57779:2035..21071622930938.0038848-CDS-H-elongata_contig57779:2035..2107Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57779 2036..2107 -
1691679632.3759408-CDS-H-elongata_contig57779:2035..21071691679632.3759408-CDS-H-elongata_contig57779:2035..2107Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57779 2036..2107 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig57779.13247.1

>prot_H-elongata_contig57779.13247.1 ID=prot_H-elongata_contig57779.13247.1|Name=mRNA_H-elongata_contig57779.13247.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=145bp
SGEVEDSQEDKDPELIGCPFYSNHVLAQQAQLVIVTYNYVLDPNIRSAVS
LDLNDAIVVIDEAHNVEGVCRDAGSVELSVVDTANIAIDLCHLCQRTAYR
EDALVLLNFVLDLLRFTAWESGNLRDFEDETTFDDPLGLLEGEEV
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mRNA from alignment at H-elongata_contig57779:284..2107-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig57779.13247.1 ID=mRNA_H-elongata_contig57779.13247.1|Name=mRNA_H-elongata_contig57779.13247.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1824bp|location=Sequence derived from alignment at H-elongata_contig57779:284..2107- (Himanthalia elongata Himel1 dioecious)
TCGGGAGAAGTGGAGGATTCACAGGAGGACAAGGACCCCGAGCTGATCGG
ATGCCCGTTCTATTCAAACCATGTACGGCGTATGCTTTTTCTTCCACCTG
AAATTCGGGTGTGTCGGTGATGTGTCCGTCTCAGTTTTCGGTGGTTATGA
GAAAGAATACTAGCCGATGGCACGCCTCGTGGTCGTTGCTTGGACGATCC
GAGCACGAATGAACGGATACAGTACGACGGAGCGAAGTGATTGCATCGTT
CTTCGCATCGTTCTTCGCATCATTCTTCGCATCGTTCTTCTCTCTTCGTG
TAACTGTTGTTTTTTCCGTTTCGACCCTGTGCGCGTGGCTTCTCAGGTGC
TAGCGCAGCAGGCACAGCTGGTCATCGTAACCTACAACTACGTGCTGGTG
AGAGGTCACACAGATATGAAAATATACCGCCACCCCTTTTTGTACGGTCC
CGCAACCCCTTTTTGCTGCGCAAACGTGCTTTTTATTTCTTCTTCTTTAG
TTGGGTGCCGTCAATGAGGAAGGAGCGTAGGCCTACGCGAAGATGGAGCG
CGCAAAAGCGGAAAAAAACGACTCATCTGGATCGCTTCTCTCGACAGTAG
CATCATCTACAGATGTTTGCTGTGCTGTGAATGCTGTGCTGTGCTGTGCT
CTCACGCGGTCGATTTCGAAATGATGTGGTAGCAGAATCGGGTCTTTTTG
TGGGCCATGTTTTGGGTGGATTCGGGGACGGTTCGAGACGTGCCCTGCAT
GGGGATGGGCGTTATGCAGTGTGTCCTGTGAGCTCAAAGACCTCGGTCGA
AGGGTCTTGTCGCTCCTCGAAGGGGTGAAACGCCTAATATTCTATAGGAT
GCCTTCTGAATAAATGATTTGTTGGAAAGCCATACCGCAGCCGCAGGCTT
GTCTCTCCCCCACAAGTAAAATCATGAACACCTTTTAGCCTGATTGTATC
ACATATATACCGTCGTCTATGTGATGTGCTCGAGCGAAGATCGAAGTCCC
TTCCCGGTACTTGGGATTTAGAGTAGAGTTGTGCCCGCTGAAAGTGGATG
CAACATTTGGTTACTCGGTGAATCCAATGAACGGGTTATTAACCCATGAT
ATATCCGATTTGGCGAAGCAAATGCAAGCATGATGACTAAAAGCTTGTAT
CATTCTTGTAAAACGCGACTTGGACGGCGAAATACCGGCATTGTTGGGAT
GCGTAGTCTATATGCCTGTTTTGGGATAAGACACGTGTTTAACATAATAC
CGCCTCTTCCACAATCAGTCACTTATTGTCTGCATATAAGTCTCATAATT
CCCCCCCAAAAAATTAGTGTTGCTGTGACCCTGCCGCAATTCGTGTGCGC
TGATGCGGCGGAGGCGTAGGACCCGAACATCCGTTCAGCGGTGTCGCTGG
ACCTGAACGACGCGATCGTCGTCATCGACGAAGCGCACAACGTAGAAGGC
GTTTGCAGAGACGCGGGATCGGTGGAGCTGTCCGTGGTGGACACGGCCAA
CATCGCCATCGATCTGTGCCATCTGTGCCAGAGGACGGCATACAGGTAGA
TCGCCTAGCTTCTTCTGCTGTCGACCCGTTGTGGATCTTCTCCGAACCAG
CGAATCACCCATAATTCGGGAGTAGTAGCATCCAACCCAACCCGCCAAAT
CTGCGGGCGCTGCTTCTCTCGATTATTTTCTGTCCCAGGGAAGATGCTTT
GGTGCTGTTGAACTTTGTGTTGGATCTCTTGCGCTTCACCGCGTGGGAAT
CAGGGAATCTCCGAGATTTCGAAGACGAGACAACCTTCGATGATCCTCTC
GGGCTGCTGGAGGGCGAGGAAGTC
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Coding sequence (CDS) from alignment at H-elongata_contig57779:284..2107-

>mRNA_H-elongata_contig57779.13247.1 ID=mRNA_H-elongata_contig57779.13247.1|Name=mRNA_H-elongata_contig57779.13247.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=870bp|location=Sequence derived from alignment at H-elongata_contig57779:284..2107- (Himanthalia elongata Himel1 dioecious)
TCGGGAGAAGTGGAGGATTCACAGGAGGACAAGGACCCCGAGCTGATCGG
ATGCCCGTTCTATTCAAACCATTCGGGAGAAGTGGAGGATTCACAGGAGG
ACAAGGACCCCGAGCTGATCGGATGCCCGTTCTATTCAAACCATGTGCTA
GCGCAGCAGGCACAGCTGGTCATCGTAACCTACAACTACGTGCTGGTGCT
AGCGCAGCAGGCACAGCTGGTCATCGTAACCTACAACTACGTGCTGGACC
CGAACATCCGTTCAGCGGTGTCGCTGGACCTGAACGACGCGATCGTCGTC
ATCGACGAAGCGCACAACGTAGAAGGCGTTTGCAGAGACGCGGGATCGGT
GGAGCTGTCCGTGGTGGACACGGCCAACATCGCCATCGATCTGTGCCATC
TGTGCCAGAGGACGGCATACAGGACCCGAACATCCGTTCAGCGGTGTCGC
TGGACCTGAACGACGCGATCGTCGTCATCGACGAAGCGCACAACGTAGAA
GGCGTTTGCAGAGACGCGGGATCGGTGGAGCTGTCCGTGGTGGACACGGC
CAACATCGCCATCGATCTGTGCCATCTGTGCCAGAGGACGGCATACAGGG
AAGATGCTTTGGTGCTGTTGAACTTTGTGTTGGATCTCTTGCGCTTCACC
GCGTGGGAATCAGGGAATCTCCGAGATTTCGAAGACGAGACAACCTTCGA
TGATCCTCTCGGGCTGCTGGAGGGCGAGGAAGTCGGAAGATGCTTTGGTG
CTGTTGAACTTTGTGTTGGATCTCTTGCGCTTCACCGCGTGGGAATCAGG
GAATCTCCGAGATTTCGAAGACGAGACAACCTTCGATGATCCTCTCGGGC
TGCTGGAGGGCGAGGAAGTC
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