mRNA_H-elongata_contig57735.13242.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig57735.13242.1
Unique NamemRNA_H-elongata_contig57735.13242.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig57735.13242.1 vs. uniprot
Match: A0A6H5KCR6_9PHAE (DUF547 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KCR6_9PHAE)

HSP 1 Score: 188 bits (478), Expect = 2.460e-53
Identity = 87/106 (82.08%), Postives = 98/106 (92.45%), Query Frame = 1 
Query:    1 RWMPLQGLSHAEQLSVFLNLYHVMLLHAYFLHGPPSSSLRAASFFTTLCYEVGGELLSMADLEHCVLRARTSQPKQLLSKLIIPSTEYPFSLRQPEPRVSFALNCG 318
            RWMPL+GLSH EQL+VFLNLYHVMLLHA+F+ GPP S LR AS+FTTLCYEVGG+++SMADLEHCVLRA+TSQP Q LSKLIIP+TEYPFSLR+ EPRVSFALNCG
Sbjct: 1401 RWMPLEGLSHNEQLAVFLNLYHVMLLHAFFILGPPGSPLRVASYFTTLCYEVGGDVMSMADLEHCVLRAKTSQPNQFLSKLIIPTTEYPFSLRRAEPRVSFALNCG 1506
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BLAST of mRNA_H-elongata_contig57735.13242.1 vs. uniprot
Match: D8LMK0_ECTSI (DUF547 domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LMK0_ECTSI)

HSP 1 Score: 187 bits (474), Expect = 8.490e-53
Identity = 85/106 (80.19%), Postives = 98/106 (92.45%), Query Frame = 1 
Query:    1 RWMPLQGLSHAEQLSVFLNLYHVMLLHAYFLHGPPSSSLRAASFFTTLCYEVGGELLSMADLEHCVLRARTSQPKQLLSKLIIPSTEYPFSLRQPEPRVSFALNCG 318
            RWMPL+GLSH+EQL+VFLNLYHVMLLHA+F+ GPP S LR AS+FTTLCYEVGG+++SMADLEHCV+RA+TSQP Q LSKLIIP+TEYPF LR+ EPRVSFALNCG
Sbjct: 1494 RWMPLEGLSHSEQLAVFLNLYHVMLLHAFFILGPPGSPLRVASYFTTLCYEVGGDVMSMADLEHCVMRAKTSQPNQFLSKLIIPTTEYPFCLRRAEPRVSFALNCG 1599
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BLAST of mRNA_H-elongata_contig57735.13242.1 vs. uniprot
Match: A0A835Z2G2_9STRA (DUF547 domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z2G2_9STRA)

HSP 1 Score: 133 bits (334), Expect = 5.070e-36
Identity = 63/107 (58.88%), Postives = 83/107 (77.57%), Query Frame = 1 
Query:    1 RWMPLQGLSH-AEQLSVFLNLYHVMLLHAYFLHGPPSSSLRAASFFTTLCYEVGGELLSMADLEHCVLRARTSQPKQLLSKLIIPSTEYPFSLRQPEPRVSFALNCG 318
            RW+ L G    +E+L++FLNLYHV+L+HA+ L GPP S+L+AAS+F +L YEV G++LS+A+LEH V+RA  S P+Q LSK  +PS  Y F+L  PEPRVSFALNCG
Sbjct:   77 RWLDLSGPGDPSERLALFLNLYHVLLMHAHLLVGPPGSALKAASYFNSLSYEVAGDVLSLAELEHGVIRAPLSAPRQCLSKFFLPSERYAFALTAPEPRVSFALNCG 183
[Close]
BLAST of mRNA_H-elongata_contig57735.13242.1 vs. uniprot
Match: A0A836C9S5_9STRA (DUF547 domain-containing protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C9S5_9STRA)

HSP 1 Score: 121 bits (303), Expect = 2.210e-33
Identity = 56/111 (50.45%), Postives = 81/111 (72.97%), Query Frame = 1 
Query:    1 RWMPLQ-----GLSHAEQLSVFLNLYHVMLLHAYFLHGPPSSSLRAASFFTTLCYEVGGELLSMADLEHCVLRARTSQPKQLLSKLIIPSTEYPFSLRQPEPRVSFALNCG 318
            RW+ L      G   +E+L++FLNLYH+M+LHAY L GPP  +++ +S+ + L YE+GG++LS+A+LEHCV+RA  +  KQ  SKL++PS +Y F+L   EPRV+FAL CG
Sbjct:   12 RWLELPRAQPGGADESERLALFLNLYHLMVLHAYLLVGPPGGAIKWSSYLSGLSYEIGGDVLSLAELEHCVIRAAAAPAKQYPSKLLVPSEKYAFALSAVEPRVNFALCCG 122
[Close]
BLAST of mRNA_H-elongata_contig57735.13242.1 vs. uniprot
Match: A0A7S3SJG3_EMIHU (Hypothetical protein n=1 Tax=Emiliania huxleyi TaxID=2903 RepID=A0A7S3SJG3_EMIHU)

HSP 1 Score: 113 bits (283), Expect = 4.100e-28
Identity = 51/102 (50.00%), Postives = 74/102 (72.55%), Query Frame = 1 
Query:   13 LQGLSHAEQLSVFLNLYHVMLLHAYFLHGPPSSSLRAASFFTTLCYEVGGELLSMADLEHCVLRARTSQPKQLLSKLIIPSTEYPFSLRQPEPRVSFALNCG 318
            L GL   ++L+ FLN+YH M+ HAY L GPPSS L+A SFF T+ Y+VGG++ ++A+LEH VLRA + +PK   SK ++P + Y F+L   + R++FAL+CG
Sbjct:  106 LDGLDEEQKLAFFLNVYHTMIQHAYSLLGPPSSLLKAISFFNTIAYQVGGDVFTLAELEHNVLRAPSCRPKSFFSKWVLPKSSYSFALTTADYRLNFALSCG 207
[Close]
BLAST of mRNA_H-elongata_contig57735.13242.1 vs. uniprot
Match: A0A7R9Y7B0_9STRA (Hypothetical protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9Y7B0_9STRA)

HSP 1 Score: 111 bits (277), Expect = 6.090e-28
Identity = 46/104 (44.23%), Postives = 74/104 (71.15%), Query Frame = 1 
Query:    7 MPLQGLSHAEQLSVFLNLYHVMLLHAYFLHGPPSSSLRAASFFTTLCYEVGGELLSMADLEHCVLRARTSQPKQLLSKLIIPSTEYPFSLRQPEPRVSFALNCG 318
            + LQ LSH E++  F+N YH++L+HAY + GPP  + + A +F  + YE G ++ S+A+LEH ++RA  + P Q LSKL++P++ Y ++L +PE R++F LNCG
Sbjct:   49 LDLQVLSHLEKVCFFVNTYHLLLMHAYLVLGPPRQAAKWARYFNAIAYECGDDIFSLAELEHNIIRAPMAAPSQFLSKLVLPTSVYKYALTEPEMRINFVLNCG 152
[Close]
BLAST of mRNA_H-elongata_contig57735.13242.1 vs. uniprot
Match: A0A7R9Y7X5_9STRA (Hypothetical protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9Y7X5_9STRA)

HSP 1 Score: 111 bits (277), Expect = 8.210e-28
Identity = 46/104 (44.23%), Postives = 74/104 (71.15%), Query Frame = 1 
Query:    7 MPLQGLSHAEQLSVFLNLYHVMLLHAYFLHGPPSSSLRAASFFTTLCYEVGGELLSMADLEHCVLRARTSQPKQLLSKLIIPSTEYPFSLRQPEPRVSFALNCG 318
            + LQ LSH E++  F+N YH++L+HAY + GPP  + + A +F  + YE G ++ S+A+LEH ++RA  + P Q LSKL++P++ Y ++L +PE R++F LNCG
Sbjct:   49 LDLQVLSHLEKVCFFVNTYHLLLMHAYLVLGPPRQAAKWARYFNAIAYECGDDIFSLAELEHNIIRAPMAAPSQFLSKLVLPTSVYKYALTEPEMRINFVLNCG 152
[Close]
BLAST of mRNA_H-elongata_contig57735.13242.1 vs. uniprot
Match: R1DA17_EMIHU (DUF547 domain-containing protein n=1 Tax=Emiliania huxleyi TaxID=2903 RepID=R1DA17_EMIHU)

HSP 1 Score: 113 bits (283), Expect = 4.740e-27
Identity = 51/102 (50.00%), Postives = 74/102 (72.55%), Query Frame = 1 
Query:   13 LQGLSHAEQLSVFLNLYHVMLLHAYFLHGPPSSSLRAASFFTTLCYEVGGELLSMADLEHCVLRARTSQPKQLLSKLIIPSTEYPFSLRQPEPRVSFALNCG 318
            L GL   ++L+ FLN+YH M+ HAY L GPPSS L+A SFF T+ Y+VGG++ ++A+LEH VLRA + +PK   SK ++P + Y F+L   + R++FAL+CG
Sbjct:  855 LDGLDEEQKLAFFLNVYHTMIQHAYSLLGPPSSLLKAISFFNTIAYQVGGDVFTLAELEHNVLRAPSCRPKSFFSKWVLPKSSYSFALTTADYRLNFALSCG 956
[Close]
BLAST of mRNA_H-elongata_contig57735.13242.1 vs. uniprot
Match: A0A836CH08_9STRA (DUF547 domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CH08_9STRA)

HSP 1 Score: 113 bits (283), Expect = 4.800e-27
Identity = 52/100 (52.00%), Postives = 75/100 (75.00%), Query Frame = 1 
Query:   19 GLSHAEQLSVFLNLYHVMLLHAYFLHGPPSSSLRAASFFTTLCYEVGGELLSMADLEHCVLRARTSQPKQLLSKLIIPSTEYPFSLRQPEPRVSFALNCG 318
            G   +E+L++FLNLYH+M+LHAY L GPP  +++  ++F  L YE   ++LS+A+LEH VLRA  + P+Q ++KL+IPS  Y F+L  PEPR+SFAL+CG
Sbjct: 1226 GGDGSERLALFLNLYHLMVLHAYLLVGPPGGAIKWPAYFNGLSYEFAHDVLSLAELEHKVLRAPLTPPQQFMAKLVIPSEGYRFALTVPEPRISFALSCG 1325
[Close]
BLAST of mRNA_H-elongata_contig57735.13242.1 vs. uniprot
Match: B7FYB5_PHATC (tRNA-intron lyase n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7FYB5_PHATC)

HSP 1 Score: 110 bits (275), Expect = 5.770e-26
Identity = 42/101 (41.58%), Postives = 73/101 (72.28%), Query Frame = 1 
Query:   16 QGLSHAEQLSVFLNLYHVMLLHAYFLHGPPSSSLRAASFFTTLCYEVGGELLSMADLEHCVLRARTSQPKQLLSKLIIPSTEYPFSLRQPEPRVSFALNCG 318
            +GL    +L+ FLN+YH M++HA+ + GPP SSL+   +F  + YEVG ++ S+ +LEHC++R++ + P Q +S+ ++P ++Y F+L + + R++FALNCG
Sbjct:  833 RGLPEESRLAFFLNVYHTMIMHAFLVLGPPGSSLKWIGYFNNIAYEVGDDVFSLTELEHCIIRSKMAYPSQFISRFVLPKSQYAFALTKADYRINFALNCG 933
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig57735.13242.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KCR6_9PHAE2.460e-5382.08DUF547 domain-containing protein n=1 Tax=Ectocarpu... [more]
D8LMK0_ECTSI8.490e-5380.19DUF547 domain-containing protein n=1 Tax=Ectocarpu... [more]
A0A835Z2G2_9STRA5.070e-3658.88DUF547 domain-containing protein n=1 Tax=Tribonema... [more]
A0A836C9S5_9STRA2.210e-3350.45DUF547 domain-containing protein (Fragment) n=1 Ta... [more]
A0A7S3SJG3_EMIHU4.100e-2850.00Hypothetical protein n=1 Tax=Emiliania huxleyi Tax... [more]
A0A7R9Y7B0_9STRA6.090e-2844.23Hypothetical protein n=1 Tax=Pinguiococcus pyrenoi... [more]
A0A7R9Y7X5_9STRA8.210e-2844.23Hypothetical protein n=1 Tax=Pinguiococcus pyrenoi... [more]
R1DA17_EMIHU4.740e-2750.00DUF547 domain-containing protein n=1 Tax=Emiliania... [more]
A0A836CH08_9STRA4.800e-2752.00DUF547 domain-containing protein n=1 Tax=Tribonema... [more]
B7FYB5_PHATC5.770e-2641.58tRNA-intron lyase n=2 Tax=Phaeodactylum tricornutu... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig57735contigH-elongata_contig57735:1312..3742 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryno signal peptide or anchor
Exons3
Model size318
Cds size180
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig57735.13242.1prot_H-elongata_contig57735.13242.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig57735 1314..1987 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930937.1785321-UTR-H-elongata_contig57735:1311..13131622930937.1785321-UTR-H-elongata_contig57735:1311..1313Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig57735 1312..1313 +
1691679632.083768-UTR-H-elongata_contig57735:1311..13131691679632.083768-UTR-H-elongata_contig57735:1311..1313Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig57735 1312..1313 +
1622930937.2425692-UTR-H-elongata_contig57735:1987..20331622930937.2425692-UTR-H-elongata_contig57735:1987..2033Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig57735 1988..2033 +
1691679632.1236796-UTR-H-elongata_contig57735:1987..20331691679632.1236796-UTR-H-elongata_contig57735:1987..2033Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig57735 1988..2033 +
1622930937.2612298-UTR-H-elongata_contig57735:3652..37421622930937.2612298-UTR-H-elongata_contig57735:3652..3742Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig57735 3653..3742 +
1691679632.1335342-UTR-H-elongata_contig57735:3652..37421691679632.1335342-UTR-H-elongata_contig57735:3652..3742Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig57735 3653..3742 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930937.2004838-CDS-H-elongata_contig57735:1313..14301622930937.2004838-CDS-H-elongata_contig57735:1313..1430Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57735 1314..1430 +
1691679632.1005437-CDS-H-elongata_contig57735:1313..14301691679632.1005437-CDS-H-elongata_contig57735:1313..1430Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57735 1314..1430 +
1622930937.224079-CDS-H-elongata_contig57735:1924..19871622930937.224079-CDS-H-elongata_contig57735:1924..1987Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57735 1925..1987 +
1691679632.1107209-CDS-H-elongata_contig57735:1924..19871691679632.1107209-CDS-H-elongata_contig57735:1924..1987Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig57735 1925..1987 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig57735.13242.1

>prot_H-elongata_contig57735.13242.1 ID=prot_H-elongata_contig57735.13242.1|Name=mRNA_H-elongata_contig57735.13242.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=60bp
MDAVAGSFSCRAAVRFSQPVSRHALARLLPPWPPQQLLEGCEFLHHAMLR
GRRRIVVNG*
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mRNA from alignment at H-elongata_contig57735:1312..3742+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig57735.13242.1 ID=mRNA_H-elongata_contig57735.13242.1|Name=mRNA_H-elongata_contig57735.13242.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2431bp|location=Sequence derived from alignment at H-elongata_contig57735:1312..3742+ (Himanthalia elongata Himel1 dioecious)
AGATGGATGCCGTTGCAGGGTCTTTCTCATGCCGAGCAGCTGTCCGTTTT
TCTCAACCTGTATCACGTCATGCTCTTGCACGCCTACTTCCTCCATGGCC
CCCCCAGCAGCTCCTTGAGGTACGCTGGCGTCCGAGAACATGCTGCGTGT
ACCGCGAGATAATGCAGCCAAATATTTAGTGATGTCTGGGTAGAATCAAG
TCGTTTTGTCATGGCTGCGATGATCGTCACACCCTTTCTTGCTTTGAGCA
GCATATGGCCCCTGGCCTCGACTCCTTCCAGAATATCTCGTTTGCATCTT
GCATGTATGTATGTATGTCTGGTCTCACTTTCAATAGAAAGATCAACCGG
ATTTGGTTTCCTCCTGCCCGTGGTCAGGGGAAAAATTATTTTCCCTGTTA
CATATGTCGCATCGATATTTGTCCTGGAACTTTCTAAACTGCCCCTCCTG
AGCCAAGCGGTTGAATATTAGTGATATCTTCGTTGAATGATATAGGGCAA
GTAGACAGGTTGTGTGTCTGGTCGGTCATCAAAATATTATGCTGAAAACT
GGTGGTGCTGCCTTTTGGCCATTGTCATGTACCTCGCTCTGATTTTATCT
TTGTACCCCGAAGGGCTGCGAGTTTCTTCACCACGCTATGCTACGAGGTC
GGCGGCGAATTGTTGTCAATGGCTGATCTCGAGCACTGTGTCCTGCGAGC
CAGGACCAGCCAGCCCAAGCAGGTAAGAATCCGTTGCGATTCATCCAAGG
TTTTCATCAAATCTCTTAAAATGGTACTGCCCCTCGAACGAACAACCAAT
ACACCGATATGAGGGGGAGACGCTTAAGTGTCCCCGATTTGGGGATGGGA
GATTAAAAACTTCGGCCTGTTCCGGGGACCCAATAGATACATGATCACCG
TAGGAGGGATGGTTAGTTTTTTTCCAGCGAAGGGATTTTTTTTAACCTGT
CTCCTCCCAGAGTGGGGAACGTTTAAGCATTTGTTCCCTCTACAATTCGA
GTGATCGATCGCTGACTCAAGCTACCTTGAGGAGTCCTGGTCCAGTGCCA
GCGGACTCTCAGTGGTGAACACAATCGGTTCGCAATTGCATGACCTGATG
AACTCGGGATTGGCCCGAATACGGTTGACGGCCGAGATGGACGACGTCGC
GGAAAGCGAGAGAATCCCAGAAGTACGCACCAGATGAATCCTGGGTTGGG
AGAATGAGCGGGCTGACGAGGGACGGGACGGCCGAACTCGTCTCCCGAGA
TCAAAATCTCAGGCACGAACGGGAACAGGGAAAAATCATTTTCCCTGTTC
AGCTGACCACGGGCAGGAGGCAACCATATCCGGTTGGTCTATACTCTATT
GCTTTATATGATTTGATGTCCACACGATAGACTGTCACATGAAAGCGGAG
GTAAAACTCTTGGTGGGCCTCGCCGAGCTTTTGCGTTGACTTGTCTGGCA
AAAACCTCCACCAAGCATCGAGCCGGATTCCCAATGGTAGTCGCATTGGA
TAAACATATTTACATCTCCGACAGGCTTGAGCCAATCGCCCCTTGCGGGG
ATACCCGAGACTAGCGAAATATTGGGGGCGGTTAAGGTTGCCTGTCATCG
TGTTGGCCAGCGACGAAGGACAATATTTCCGCCACACTAAGGAGAAGCAG
GGTGTATTCGCTCGCGATACCGGGAGTGCCTAGAGGGTAGGCGGACGCAC
ATTCCGAGCGTTTTTGGCTGAGATTTGTCAAAGGACAGAAGCCAGCGTTA
AACCTCCAGCCTCCGCTGTAGAAGCACTCTTCCACAGCTCTAGTTTGGCG
TTGAAGATGCTTTGGTCCCGCTCACGTTACCGATATGAGGCTCGTTGGTC
TCTGGGCCGAATTTATTTTCCCAACACGCTCCTGCGGTGGGCTCTTAATG
ATTGGTTGGAGCTATGCTTCTCTTTACAACATATATGGCTCATTAATGCG
AGAGGGGTATTTGAGAAAGGAATCAGCACCCAAGTTCTTGGGAAGACGCT
ATCAAAACAGCCCAAACCACTTCTTAATCTTCCATATGTTGGAATAAATA
GTCGATTCGGTGATGAGATGGGTGGGGGAGATGTTTTCACGCAGTCCCCT
TTTTGACTTGGGGACATTGTGTCCAACCCCCCCCTGCTTTGTTCCTTTTG
AGGACACGCGGTCCATGGCCTCACAACAAAGTCATGTAAAACTTAAGTAC
GCCCCTTCTCCCCATGCCTTACCTTCCTCTCCCTATCCCTGGATTACTCT
ATGGAGGAAAAGTGCGCGTGTCAAAGCTACCACATTTTCCGTCCCATGAT
GAACCGCGTCCTCGTCTCTTTTCTCTCCTGCGTGTCCCTAGCTCCTGTCC
AAGCTCATCATCCCTTCGACGGAGTATCCATTTTCCTTGAGACAACCCGA
ACCTAGGGTTAGTTTTGCCCTCAACTGCGGC
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Coding sequence (CDS) from alignment at H-elongata_contig57735:1312..3742+

>mRNA_H-elongata_contig57735.13242.1 ID=mRNA_H-elongata_contig57735.13242.1|Name=mRNA_H-elongata_contig57735.13242.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=360bp|location=Sequence derived from alignment at H-elongata_contig57735:1312..3742+ (Himanthalia elongata Himel1 dioecious)
ATGGATGCCGTTGCAGGGTCTTTCTCATGCCGAGCAGCTGTCCGTTTTTC
TCAACCTGTATCACGTCATGCTCTTGCACGCCTACTTCCTCCATGGCCCC
CCCAGCAGCTCCTTGAGATGGATGCCGTTGCAGGGTCTTTCTCATGCCGA
GCAGCTGTCCGTTTTTCTCAACCTGTATCACGTCATGCTCTTGCACGCCT
ACTTCCTCCATGGCCCCCCCAGCAGCTCCTTGAGGGCTGCGAGTTTCTTC
ACCACGCTATGCTACGAGGTCGGCGGCGAATTGTTGTCAATGGCTGAGGC
TGCGAGTTTCTTCACCACGCTATGCTACGAGGTCGGCGGCGAATTGTTGT
CAATGGCTGA
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