mRNA_H-elongata_contig110516.869.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig110516.869.1
Unique NamemRNA_H-elongata_contig110516.869.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig110516.869.1 vs. uniprot
Match: A0A6H5KEX0_9PHAE (Isochorismatase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KEX0_9PHAE)

HSP 1 Score: 162 bits (410), Expect = 3.830e-47
Identity = 79/118 (66.95%), Postives = 93/118 (78.81%), Query Frame = 1 
Query:   10 VSGTSRFMLDVSKALGVPCVVTEQYPKALLHTVSELGVGEGGERTNVPVFEKKLFSMCTDDVMSHMDALANKPTDVVLFGIEAHVCVTQTCLDLLERGYGVHIVCDGVSSQKVQSFSV 363
            V  T++FMLDVS+ L VPC+VTEQYPKALLHTV EL VG G ++ NVP+FEKKLFSMCT +V   +  L ++  D VLFGIEAHVCVTQTCLDLL+ GY VH+VCDGVSSQK    +V
Sbjct:   39 VISTAKFMLDVSQELEVPCIVTEQYPKALLHTVPELNVGNGPDQINVPIFEKKLFSMCTKEVEEELKKLPDRK-DAVLFGIEAHVCVTQTCLDLLDLGYEVHVVCDGVSSQKPHDRAV 155
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BLAST of mRNA_H-elongata_contig110516.869.1 vs. uniprot
Match: D7FJG0_ECTSI (Isochorismatase domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FJG0_ECTSI)

HSP 1 Score: 163 bits (412), Expect = 1.120e-46
Identity = 79/118 (66.95%), Postives = 94/118 (79.66%), Query Frame = 1 
Query:   10 VSGTSRFMLDVSKALGVPCVVTEQYPKALLHTVSELGVGEGGERTNVPVFEKKLFSMCTDDVMSHMDALANKPTDVVLFGIEAHVCVTQTCLDLLERGYGVHIVCDGVSSQKVQSFSV 363
            V  T++FMLDVS+ L VPC+VTEQYPKALLHTV EL VG+G ++ NVP+FEKKLFSMCT +V   +  L ++  D VLFGIEAHVCVTQTCLDLL+ GY VH+VCDGVSSQK    +V
Sbjct:  101 VISTAKFMLDVSQELEVPCIVTEQYPKALLHTVPELNVGKGPDQINVPIFEKKLFSMCTKEVEEELKKLPDRK-DAVLFGIEAHVCVTQTCLDLLDLGYDVHVVCDGVSSQKPHDRAV 217
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BLAST of mRNA_H-elongata_contig110516.869.1 vs. uniprot
Match: A0A6U3TC81_9STRA (Hypothetical protein n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A6U3TC81_9STRA)

HSP 1 Score: 118 bits (295), Expect = 6.850e-30
Identity = 57/116 (49.14%), Postives = 80/116 (68.97%), Query Frame = 1 
Query:    1 MFLVSGTSRFMLDVSKALGVPCVVTEQYPKALLHTVSELGVGEGGERTNVPVFEKKLFSMCTDDVMSHMDALAN-KPTDVVLFGIEAHVCVTQTCLDLLERGYGVHIVCDGVSSQK 345
            M  V  T++FM  V+K L +PCV T+QY K    T+ +         T++PVFEKKLFSMCT++   H+ +L++ + T  ++ GIEAHVC+ QTCLDLLE+G+ VHI+ DGVSSQ+
Sbjct:   35 METVINTTKFMTAVAKELQIPCVATQQYTKVFGPTIPDAIADPNDIGTSIPVFEKKLFSMCTEECSKHLASLSSSEKTSYLIVGIEAHVCLQQTCLDLLEQGHDVHIIADGVSSQQ 150
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BLAST of mRNA_H-elongata_contig110516.869.1 vs. uniprot
Match: A0A6S7ZR19_9STRA (Hypothetical protein n=1 Tax=Aplanochytrium stocchinoi TaxID=215587 RepID=A0A6S7ZR19_9STRA)

HSP 1 Score: 117 bits (293), Expect = 1.360e-29
Identity = 61/115 (53.04%), Postives = 76/115 (66.09%), Query Frame = 1 
Query:   19 TSRFMLDVSKALGVPCVVTEQYPKALLHTVSELGVGEGGERTNVPVFEKKLFSMCTDDVMSHMDALANKPTDVVLFGIEAHVCVTQTCLDLLERGYGVHIVCDGVSSQKVQSFSV 363
            TS  M+  SK +G+PCVVTEQYPKAL HT SE+  G+      + VFEK LFSM T +V  H+  L       V+FG+E HVCV QT LDLLE+G+ VH++ DGVSSQ+    SV
Sbjct:   42 TSSIMVKASKIMGIPCVVTEQYPKALGHTCSEVLAGDTSPL--IKVFEKSLFSMMTPEVEQHLKDLNRN--SAVIFGVETHVCVQQTALDLLEKGWDVHVIVDGVSSQREFDRSV 152
[Close]
BLAST of mRNA_H-elongata_contig110516.869.1 vs. uniprot
Match: A0A813L2S5_POLGL (Hypothetical protein (Fragment) n=1 Tax=Polarella glacialis TaxID=89957 RepID=A0A813L2S5_POLGL)

HSP 1 Score: 114 bits (284), Expect = 1.010e-28
Identity = 57/109 (52.29%), Postives = 77/109 (70.64%), Query Frame = 1 
Query:   19 TSRFMLDVSKALGVPCVVTEQYPKALLHTVSELGVGEGGERTNVPVFEKKLFSMCTDDVMSHMDALANKPTDVVLFGIEAHVCVTQTCLDLLERGYGVHIVCDGVSSQK 345
            +++ M+  ++   VP +V+EQYPKAL HTVSEL +  G       VFEK  FSMCT++ ++H+++L  K   VV+FG+E HVCV QT LDLL RGY VHI+ DGVSSQ+
Sbjct:   33 SAKTMVQGAEIFDVPLLVSEQYPKALGHTVSELNITRG------KVFEKTKFSMCTNEFLAHLESLGRKA--VVIFGVETHVCVQQTALDLLSRGYQVHILADGVSSQR 133
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BLAST of mRNA_H-elongata_contig110516.869.1 vs. uniprot
Match: A0A6G0UJF7_9BILA (Isochorismatase domain-containing protein n=1 Tax=Halicephalobus sp. NKZ332 TaxID=2598192 RepID=A0A6G0UJF7_9BILA)

HSP 1 Score: 114 bits (285), Expect = 1.830e-28
Identity = 58/109 (53.21%), Postives = 75/109 (68.81%), Query Frame = 1 
Query:   22 SRFMLDVSKALGVPCVVTEQYPKALLHTVSELGVGEGGERTNVPVFEKKLFSMCTDDVMSHMDALANKPTDVVLFGIEAHVCVTQTCLDLLERGYGVHIVCDGVSSQKV 348
            SR ++D SK L VP V TEQYPK L H+V+ELG+ E     N+ VF K  FSMC ++++  ++    K  ++VL GIEAHVCV QTCLDLL RGY VH+V D VSS+ +
Sbjct:   39 SRRLIDASKILNVPIVATEQYPKGLGHSVAELGLQEN----NIQVFSKMCFSMCANEILGKLEKEQVK--EIVLCGIEAHVCVYQTCLDLLSRGYTVHVVADAVSSRSM 141
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BLAST of mRNA_H-elongata_contig110516.869.1 vs. uniprot
Match: A0A7S1Z0I8_TRICV (Hypothetical protein (Fragment) n=2 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1Z0I8_TRICV)

HSP 1 Score: 112 bits (279), Expect = 3.550e-28
Identity = 57/104 (54.81%), Postives = 72/104 (69.23%), Query Frame = 1 
Query:   19 TSRFMLDVSKALGVPCVVTEQYPKALLHTVSELGVGEGGERTNVPVFEKKLFSMCTDDVMSHMDALANKPTDVVLFGIEAHVCVTQTCLDLLERGYGVHIVCDG 330
            T+RFM  V+KALGVP V T+QY K    TV++    +  +  + P+FEKKLFSM T +V  H+  L  + T  VLFGIEAHVCV QT LDLLE+G+GVH+V DG
Sbjct:   47 TARFMTSVAKALGVPIVATQQYTKVFGPTVADC-FADPADLDSTPIFEKKLFSMMTPEVSEHVGGLGGR-TSFVLFGIEAHVCVQQTALDLLEQGHGVHVVVDG 148
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BLAST of mRNA_H-elongata_contig110516.869.1 vs. uniprot
Match: A0A6V1K7V1_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1K7V1_HETAK)

HSP 1 Score: 112 bits (281), Expect = 1.460e-27
Identity = 59/119 (49.58%), Postives = 78/119 (65.55%), Query Frame = 1 
Query:   10 VSGTSRFMLDVSKALGVPCVVTEQYPKALLHTVSELGVG-----EGG--ERTNVPVFEKKLFSMCTDDVMSHMDALANKPTDVVLFGIEAHVCVTQTCLDLLERGYGVHIVCDGVSSQK 345
            V  TSR+++ +S+ LG+P + T+QY +A   T  E+ +      E G  + T++PVFEKKLFSM TD+V + +D        VVLFG E HVCV QTCLDLLERG  VH+V DG SSQ+
Sbjct:   35 VVNTSRYLVQISRELGIPVIATQQYTRAFGPTCPEIPLTAPEPEESGAPKATDIPVFEKKLFSMITDEVGTALDQCGPSLRSVVLFGQETHVCVQQTCLDLLERGLEVHVVADGCSSQR 153
[Close]
BLAST of mRNA_H-elongata_contig110516.869.1 vs. uniprot
Match: A0A2P6VGK8_9CHLO (Isochorismatase domain-containing 2 n=1 Tax=Micractinium conductrix TaxID=554055 RepID=A0A2P6VGK8_9CHLO)

HSP 1 Score: 112 bits (281), Expect = 1.600e-27
Identity = 64/115 (55.65%), Postives = 77/115 (66.96%), Query Frame = 1 
Query:   19 TSRFMLDVSKALGVPCVVTEQYPKALLHTVSELGVGEGGERTNVPVFEKKLFSMCTDDVMSHMDALANKPTDVVLFGIEAHVCVTQTCLDLLERGYGVHIVCDGVSSQKVQSFSV 363
            TSR ML  + AL +P +VTEQYPKAL +TV EL   +       PV  K LFSMCTD+V + ++ L      V++ GIE HVCV QTCLDLLERGY VHIV DGVSSQ++   SV
Sbjct:   73 TSRRMLRAASALQLPVIVTEQYPKALGNTVEEL---QPHIPPGSPVVAKTLFSMCTDEVNAALEGLP-AVKKVLIVGIETHVCVLQTCLDLLERGYEVHIVVDGVSSQRLGDRSV 183
[Close]
BLAST of mRNA_H-elongata_contig110516.869.1 vs. uniprot
Match: A0A2P6TFH5_CHLSO (Isochorismatase domain-containing 2 n=1 Tax=Chlorella sorokiniana TaxID=3076 RepID=A0A2P6TFH5_CHLSO)

HSP 1 Score: 112 bits (280), Expect = 2.200e-27
Identity = 62/115 (53.91%), Postives = 77/115 (66.96%), Query Frame = 1 
Query:   19 TSRFMLDVSKALGVPCVVTEQYPKALLHTVSELGVGEGGERTNVPVFEKKLFSMCTDDVMSHMDALANKPTDVVLFGIEAHVCVTQTCLDLLERGYGVHIVCDGVSSQKVQSFSV 363
            TSR ML  ++AL +P +VTEQYPKAL +TV EL         + PV  K  FSMCTD+V + +D L +    V++ GIE HVCV QT LDLLERGY VHI+ DGVSSQ++   SV
Sbjct:   71 TSRRMLRAAQALQLPVIVTEQYPKALGNTVEEL---REFIPADAPVVAKTKFSMCTDEVNAALDKLGSGVRKVLIVGIETHVCVLQTTLDLLERGYEVHILVDGVSSQRLGDRSV 182
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig110516.869.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KEX0_9PHAE3.830e-4766.95Isochorismatase domain-containing protein n=1 Tax=... [more]
D7FJG0_ECTSI1.120e-4666.95Isochorismatase domain-containing protein n=1 Tax=... [more]
A0A6U3TC81_9STRA6.850e-3049.14Hypothetical protein n=1 Tax=Skeletonema marinoi T... [more]
A0A6S7ZR19_9STRA1.360e-2953.04Hypothetical protein n=1 Tax=Aplanochytrium stocch... [more]
A0A813L2S5_POLGL1.010e-2852.29Hypothetical protein (Fragment) n=1 Tax=Polarella ... [more]
A0A6G0UJF7_9BILA1.830e-2853.21Isochorismatase domain-containing protein n=1 Tax=... [more]
A0A7S1Z0I8_TRICV3.550e-2854.81Hypothetical protein (Fragment) n=2 Tax=Trieres ch... [more]
A0A6V1K7V1_HETAK1.460e-2749.58Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A2P6VGK8_9CHLO1.600e-2755.65Isochorismatase domain-containing 2 n=1 Tax=Micrac... [more]
A0A2P6TFH5_CHLSO2.200e-2753.91Isochorismatase domain-containing 2 n=1 Tax=Chlore... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig110516contigH-elongata_contig110516:138..1948 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score163.3
Seed ortholog evalue1.1e-37
Seed eggNOG ortholog2880.D7FJG0
Preferred nameMAR1
EggNOG free text desc.isochorismatase domain-containing protein
EggNOG OGsCOG1335@1,KOG4044@2759
COG Functional cat.Q
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionIsochorismatase-like
Ec32 orthologEc-14_006200.1
Exons5
Model size567
Cds size567
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig110516.869.1prot_H-elongata_contig110516.869.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig110516 138..1948 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929632.2440882-CDS-H-elongata_contig110516:137..2271622929632.2440882-CDS-H-elongata_contig110516:137..227Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig110516 138..227 +
1691679098.9705129-CDS-H-elongata_contig110516:137..2271691679098.9705129-CDS-H-elongata_contig110516:137..227Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig110516 138..227 +
1622929632.261719-CDS-H-elongata_contig110516:441..4981622929632.261719-CDS-H-elongata_contig110516:441..498Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig110516 442..498 +
1691679098.9816923-CDS-H-elongata_contig110516:441..4981691679098.9816923-CDS-H-elongata_contig110516:441..498Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig110516 442..498 +
1622929632.2754772-CDS-H-elongata_contig110516:836..9651622929632.2754772-CDS-H-elongata_contig110516:836..965Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig110516 837..965 +
1691679098.9915004-CDS-H-elongata_contig110516:836..9651691679098.9915004-CDS-H-elongata_contig110516:836..965Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig110516 837..965 +
1622929632.2947772-CDS-H-elongata_contig110516:1307..13751622929632.2947772-CDS-H-elongata_contig110516:1307..1375Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig110516 1308..1375 +
1691679099.0125408-CDS-H-elongata_contig110516:1307..13751691679099.0125408-CDS-H-elongata_contig110516:1307..1375Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig110516 1308..1375 +
1622929632.3237252-CDS-H-elongata_contig110516:1725..19481622929632.3237252-CDS-H-elongata_contig110516:1725..1948Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig110516 1726..1948 +
1691679099.021822-CDS-H-elongata_contig110516:1725..19481691679099.021822-CDS-H-elongata_contig110516:1725..1948Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig110516 1726..1948 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig110516.869.1

>prot_H-elongata_contig110516.869.1 ID=prot_H-elongata_contig110516.869.1|Name=mRNA_H-elongata_contig110516.869.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=189bp
MFLVSGTSRFMLDVSKALGVPCVVTEQYPKALLHTVSELGVGEGGERTNV
PVFEKKLFSMCTDDVMSHMDALANKPTDVVLFGIEAHVCVTQTCLDLLER
GYGVHIVCDGVSSQKVQSFSVKHAAAPEINELIREPRPARPISSATRRKL
PSRGAKFLADENSTLSVLDADLTIEPSVCRGCSKLGHS*
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mRNA from alignment at H-elongata_contig110516:138..1948+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig110516.869.1 ID=mRNA_H-elongata_contig110516.869.1|Name=mRNA_H-elongata_contig110516.869.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1811bp|location=Sequence derived from alignment at H-elongata_contig110516:138..1948+ (Himanthalia elongata Himel1 dioecious)
ATGTTCCTTGTGTCTGGCACGTCCAGGTTCATGCTGGACGTCTCCAAGGC
GCTGGGCGTACCGTGCGTCGTCACCGAGCAGTACCCAAAGGTGTGACATG
CTACAAGCGAACCGCATTGATCCTTTTTGTACGCGAATCTGCAGCTTTCC
TATAGGATCGTCTATCTTTCTGTGAACACAGCGAACCCTCGCCCTTACAA
TAACTAGTAGTTGATTGACCCGACCACCTGACGAAACATTTACAGTTATC
AACTCTCTTGCGATACGTAACAACCTCGTTGGCCTACTGCGCTTTCATTC
CAAGGCGCTGCTGCACACTGTCTCGGAGCTAGGCGTAGGCGAAGGTGGAG
AGCGAACCAATGTGAGGAGGACAACGCAGTATGATTTCGCACCATTGAAA
TAGTCTAAAACCAAAATCCAGAACATCAGAACATCGTATTTCCTTAGGTA
CCCCTCCCCTTCCCGGCTAAGGGCACATTTGCAAGCACTCGTTTTTTTAC
GCTGAGAACGCAATGTTACGGGACGAGAGCTATCAAAATTGCTAGCGATT
CGATTTGCCGTTTCCAACACTTACTCAAAAGAGGGTGTTCTTTCTAACAT
GGTGTAACTTCCTGGAGTTCGGTGCTCAACTTTAGAATTGGCTTTGTTTT
CACAGTACAGTATATGCCGGGCCCTCCCGTCTCGAACGTGGTATCTCAGG
TGCCCGTTTTCGAGAAGAAACTATTCTCCATGTGCACCGACGATGTCATG
TCTCACATGGACGCCCTAGCGAACAAGCCTACTGACGTGGTGCTGTTTGG
GATAGAGGCGCATGTGTGTGTGACTCAGGTACAATGCGCAATGAGAAAAT
AACAATATCTTGCCTTTCAAGTGATGGGCCTTGGTCTCGCGGTTTCAATT
TATTGATACGATTTTGCCAGCGGGGTCGATAGTGCACGGATGTTTCTGTG
TGTGGTACCATAGGTTATGTCATTTCATGCGATGTTGACGTCGATTTTTA
TTACGAGCGATGCCCGGTAGAGAGGTCGCGTTAACCTCCTTAGCCTCACA
TGTTGGGGTTAAATGTATGGAGAAGACCACGCGTGCTTATCACCCCGTTT
ATTCGCCAACTGAATAGCACAGCTTGTCCATATGACGTTAATGGCCCGTC
GTCCCAACAATCGACTGTAGACTTGCCTGGATTTATTGGAGCGTGGCTAC
GGGGTCCACATCGTCTGCGATGGTGTATCTAGCCAAAAGTGAGAGAAGCC
ATGAAAGCGTTCCCCATCTGCATCACGCTTAGGAAAGCCTTGCAGAGGGA
GATATAATCCTACCGCGGGTCAAAGCAATATAATCCTACCGCAGTAAAGC
TAGAGTAACATATATTATATACAGTATAGACTAAATCTAATGGGATCGTG
TTTGTAGGCTTCGCCTGTTTTGGAAATCACCAATTGCCGGTAGGCGTGGG
AAGGAAAAGGACGGTAGTGCTTGGGGAAATTGTACGAGGGACCATCAACA
CCACCGACGGGCTACTGGTACATAGCCCAGAGCAGATCGACCCCTTACTT
ATTATTTACCTTTATGGTCTGTACCTTAACACGCTTAGGGTACAAAGCTT
CAGCGTCAAGCATGCAGCTGCACCAGAAATAAATGAACTTATCAGAGAAC
CACGGCCAGCTCGTCCAATATCCTCTGCGACAAGAAGAAAGCTTCCTTCT
CGTGGGGCAAAATTTCTAGCTGACGAGAATTCAACGCTATCCGTGCTGGA
CGCAGACCTTACGATCGAACCGTCGGTCTGCAGAGGATGCAGCAAGTTGG
GGCATTCTTGA
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Coding sequence (CDS) from alignment at H-elongata_contig110516:138..1948+

>mRNA_H-elongata_contig110516.869.1 ID=mRNA_H-elongata_contig110516.869.1|Name=mRNA_H-elongata_contig110516.869.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=1134bp|location=Sequence derived from alignment at H-elongata_contig110516:138..1948+ (Himanthalia elongata Himel1 dioecious)
ATGTTCCTTGTGTCTGGCACGTCCAGGTTCATGCTGGACGTCTCCAAGGC
GCTGGGCGTACCGTGCGTCGTCACCGAGCAGTACCCAAAGATGTTCCTTG
TGTCTGGCACGTCCAGGTTCATGCTGGACGTCTCCAAGGCGCTGGGCGTA
CCGTGCGTCGTCACCGAGCAGTACCCAAAGGCGCTGCTGCACACTGTCTC
GGAGCTAGGCGTAGGCGAAGGTGGAGAGCGAACCAATGCGCTGCTGCACA
CTGTCTCGGAGCTAGGCGTAGGCGAAGGTGGAGAGCGAACCAATGTGCCC
GTTTTCGAGAAGAAACTATTCTCCATGTGCACCGACGATGTCATGTCTCA
CATGGACGCCCTAGCGAACAAGCCTACTGACGTGGTGCTGTTTGGGATAG
AGGCGCATGTGTGTGTGACTCAGGTGCCCGTTTTCGAGAAGAAACTATTC
TCCATGTGCACCGACGATGTCATGTCTCACATGGACGCCCTAGCGAACAA
GCCTACTGACGTGGTGCTGTTTGGGATAGAGGCGCATGTGTGTGTGACTC
AGACTTGCCTGGATTTATTGGAGCGTGGCTACGGGGTCCACATCGTCTGC
GATGGTGTATCTAGCCAAAAACTTGCCTGGATTTATTGGAGCGTGGCTAC
GGGGTCCACATCGTCTGCGATGGTGTATCTAGCCAAAAGGTACAAAGCTT
CAGCGTCAAGCATGCAGCTGCACCAGAAATAAATGAACTTATCAGAGAAC
CACGGCCAGCTCGTCCAATATCCTCTGCGACAAGAAGAAAGCTTCCTTCT
CGTGGGGCAAAATTTCTAGCTGACGAGAATTCAACGCTATCCGTGCTGGA
CGCAGACCTTACGATCGAACCGTCGGTCTGCAGAGGATGCAGCAAGTTGG
GGCATTCTTGAGGTACAAAGCTTCAGCGTCAAGCATGCAGCTGCACCAGA
AATAAATGAACTTATCAGAGAACCACGGCCAGCTCGTCCAATATCCTCTG
CGACAAGAAGAAAGCTTCCTTCTCGTGGGGCAAAATTTCTAGCTGACGAG
AATTCAACGCTATCCGTGCTGGACGCAGACCTTACGATCGAACCGTCGGT
CTGCAGAGGATGCAGCAAGTTGGGGCATTCTTGA
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