mRNA_H-elongata_contig107685.632.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig107685.632.1
Unique NamemRNA_H-elongata_contig107685.632.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig107685.632.1 vs. uniprot
Match: D8LID0_ECTSI (RING-type domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LID0_ECTSI)

HSP 1 Score: 282 bits (721), Expect = 2.620e-84
Identity = 168/270 (62.22%), Postives = 186/270 (68.89%), Query Frame = 1 
Query:    1 MMFTAVTDDGVSLGSERGSELMMEGADPSCPLCNVAYEEGSDLCLLPCLHVFHENCIHKWCNRHIVCPLCKRHLEAPEPLHTLPGY----GGGMAQSEP----VGNGHDATGRQTWGHMLGTAAPGPASGHGSARGLGGTRSDDGLWNDRVLNAAQRQMRGG-HNRVTSFSSETRRHNSARGRTG---------GPPQSSSAGTSV-VGRSQSINTSSARVHQGFQRTRSSSF-----------STTVPERGRT-LGRVAHGTAPHRGGA 717
            MMFTAVTDDGVSLGSERGSELMM+GADPSCPLCN AY EG++LCLLPCLHVFH+ CI+KWCNRHIVCPLCKRHLEA EPLHTL       GG   QSEP     GNGH    R  W  +LG  APGPAS HGS RGLGGTRS+DG+WNDRVLNAAQ+Q  GG HNRVTSFSSETRR N+ARGR G         GPP SSSAG  +   R QS++ S+     G+Q+                 + T  +RGR   GRVAHGTA HR  A
Sbjct:  338 MMFTAVTDDGVSLGSERGSELMMDGADPSCPLCNKAYVEGAELCLLPCLHVFHKTCINKWCNRHIVCPLCKRHLEAAEPLHTLTASYNHPGGNAVQSEPGHRGYGNGHQ---RHQWRGVLGNTAPGPASAHGSERGLGGTRSEDGMWNDRVLNAAQQQQMGGKHNRVTSFSSETRRSNNARGRPGERVGSGRGGGPPHSSSAGAGMGPQRPQSVSGSA-----GWQQXXXXXXXXXXXXXXXXTTATEQQRGRGGSGRVAHGTARHRSSA 599
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BLAST of mRNA_H-elongata_contig107685.632.1 vs. uniprot
Match: A0A6H5JTX8_9PHAE (RING-type domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JTX8_9PHAE)

HSP 1 Score: 271 bits (692), Expect = 3.480e-80
Identity = 164/272 (60.29%), Postives = 176/272 (64.71%), Query Frame = 1 
Query:    1 MMFTAVTDDGVSLGSERGSELMMEGADPSCPLCNVAYEEGSDLCLLPCLHVFHENCIHKWCNRHIVCPLCKRHLEAPEPLHTLPGY----GGGMAQSEP----VGNGHDATGRQTWGHMLGTAAPGPASGHGSARGLGGTRSDDGLWNDRVLNAAQRQMRGG-HNRVTSFSSETRRHNSARGRTG---------GPPQSSSAGTSVVG-RSQSINTSSARVHQGFQRTRSSSFST-------------TVPERGRT-LGRVAHGTAPHRGGA 717
            MMFTAVTDDGVSLGSERGSELMM+GADPSCPLCN  Y EG++LCLLPCLHVFH+ CI+KWCNRHIVCPLCKRHLEA EPLHTL       G    QSEP     GNG     R  W  MLG AAPGPAS HGS RGLGGTRS+DG+WNDRVLNAAQRQ  GG HNRVTSFSSETRR N+ARGR G         GPP SSS        R  S++ S+ R                            T  +RGR   GRVAHGTA HR  A
Sbjct:  336 MMFTAVTDDGVSLGSERGSELMMDGADPSCPLCNKVYVEGAELCLLPCLHVFHKTCINKWCNRHIVCPLCKRHLEAAEPLHTLTASYNHPGDNTVQSEPGHRGYGNGQQ---RHQWRGMLGNAAPGPASAHGSERGLGGTRSEDGMWNDRVLNAAQRQQMGGKHNRVTSFSSETRRSNNARGRPGERAGSGRGGGPPHSSSXXXXXXXXRPHSVSGSTGRQQXXXXXXXXXXXXXXXXXXXXXXXXXXTEQQRGRGGSGRVAHGTARHRSSA 604
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BLAST of mRNA_H-elongata_contig107685.632.1 vs. uniprot
Match: A0A7S0UHT1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pseudo-nitzschia delicatissima TaxID=44447 RepID=A0A7S0UHT1_9STRA)

HSP 1 Score: 67.4 bits (163), Expect = 1.680e-9
Identity = 31/72 (43.06%), Postives = 43/72 (59.72%), Query Frame = 1 
Query:    7 FTAVTDDGVSLGSERGSELMMEGADPSCPLCNVAYEEGSDLCLLPCLHVFHENCIHKWCNRHIVCPLCKRHL 222
            F + T  G   GS+ G  L  E  +P+C +C   YE+G +L +LPC HVFHE+CI  W + ++ CPLC   L
Sbjct:  252 FKSPTSHGNLFGSDGGKTLDEE--EPTCSVCLCEYEKGDNLIMLPCKHVFHEDCIKSWTDNNLRCPLCNADL 321
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BLAST of mRNA_H-elongata_contig107685.632.1 vs. uniprot
Match: A0A1S6GLJ1_9POAL (E3 ubiquitin protein ligase RIE1-like protein (Fragment) n=1 Tax=Eleocharis dulcis TaxID=110284 RepID=A0A1S6GLJ1_9POAL)

HSP 1 Score: 60.1 bits (144), Expect = 8.530e-9
Identity = 23/45 (51.11%), Postives = 29/45 (64.44%), Query Frame = 1 
Query:   88 CPLCNVAYEEGSDLCLLPCLHVFHENCIHKWCNRHIVCPLCKRHL 222
            C +C   YE+G++LC LPC H FH  CI KW   H  CPLCK ++
Sbjct:    3 CCICLATYEDGTELCALPCNHHFHSTCIIKWLRIHATCPLCKYNI 47
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BLAST of mRNA_H-elongata_contig107685.632.1 vs. uniprot
Match: A0A0K9P9K8_ZOSMR (Putative Ring finger protein n=1 Tax=Zostera marina TaxID=29655 RepID=A0A0K9P9K8_ZOSMR)

HSP 1 Score: 64.3 bits (155), Expect = 2.240e-8
Identity = 29/74 (39.19%), Postives = 42/74 (56.76%), Query Frame = 1 
Query:    7 FTAVTDDGV--SLGSERGSELMMEGADPSCPLCNVAYEEGSDLCLLPCLHVFHENCIHKWCNRHIVCPLCKRHL 222
            F +  D G+    G+E   E  +   D  C +C  AY++G++LC LPC H FH NC+ KW   +  CPLCK ++
Sbjct:  284 FKSDLDGGIMTECGTETPYENALSAEDAECCICLSAYDDGAELCELPCSHHFHSNCVVKWLYINATCPLCKYNI 357
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BLAST of mRNA_H-elongata_contig107685.632.1 vs. uniprot
Match: A0A383VIV6_TETOB (RING-type domain-containing protein n=1 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383VIV6_TETOB)

HSP 1 Score: 62.4 bits (150), Expect = 5.410e-8
Identity = 26/60 (43.33%), Postives = 35/60 (58.33%), Query Frame = 1 
Query:   34 SLGSERGSELMMEGADPSCPLCNVAYEEGSDLCLLPCLHVFHENCIHKWCNRHIVCPLCK 213
            S  S  G E M E   P C +C  A++E   + LLPCLH++HE CI  W  R  +CP+C+
Sbjct:   46 SQNSNDGKETMTEDELPQCVICCCAFQEQEQVKLLPCLHMYHEECIDAWLRRDHICPICQ 105
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BLAST of mRNA_H-elongata_contig107685.632.1 vs. uniprot
Match: A0A7I8IBN1_SPIIN (Hypothetical protein n=1 Tax=Spirodela intermedia TaxID=51605 RepID=A0A7I8IBN1_SPIIN)

HSP 1 Score: 62.0 bits (149), Expect = 9.970e-8
Identity = 29/68 (42.65%), Postives = 38/68 (55.88%), Query Frame = 1 
Query:   10 TAVTDDGVSLGSERGSELMMEGADPSCPLCNVAYEEGSDLCLLPCLHVFHENCIHKWCNRHIVCPLCK 213
            T VTD   ++G     +L     D  C +C   YE+G++LC LPC H FH  CI KW   + +CPLCK
Sbjct:  219 TNVTDTRNTMG-----DLSHSPEDSDCCICLCRYEDGAELCSLPCNHYFHSGCITKWLRINAICPLCK 281
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BLAST of mRNA_H-elongata_contig107685.632.1 vs. uniprot
Match: A0A7I8JYG6_SPIIN (Hypothetical protein n=1 Tax=Spirodela intermedia TaxID=51605 RepID=A0A7I8JYG6_SPIIN)

HSP 1 Score: 61.6 bits (148), Expect = 1.840e-7
Identity = 25/53 (47.17%), Postives = 32/53 (60.38%), Query Frame = 1 
Query:   55 SELMMEGADPSCPLCNVAYEEGSDLCLLPCLHVFHENCIHKWCNRHIVCPLCK 213
            S+L     D  C +C   YE+G++LC LPC H FH  CI KW   + +CPLCK
Sbjct:  302 SDLSHSPEDSDCCICLCRYEDGAELCSLPCNHYFHSGCITKWLRINAICPLCK 354
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BLAST of mRNA_H-elongata_contig107685.632.1 vs. uniprot
Match: A0A8J5XKQ2_DIALT (RING-type domain-containing protein n=2 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J5XKQ2_DIALT)

HSP 1 Score: 62.0 bits (149), Expect = 1.920e-7
Identity = 27/70 (38.57%), Postives = 40/70 (57.14%), Query Frame = 1 
Query:   22 DDGVSLGSERGSELMMEGADPSCPLCNVAYEEGSDLCLLPCLHVFHENCIHKWCNRHIVCPLCKRHLEAP 231
            D   +  SER  E  ++ A  SC +C + +EE  ++C+LPC HVFH  CIH+W  +   CP C+  +  P
Sbjct:  593 DAPAARASERDDERSLD-ASVSCVVCLMEFEEEVEVCVLPCRHVFHTECIHRWLRQEGSCPQCRHRIAPP 661
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BLAST of mRNA_H-elongata_contig107685.632.1 vs. uniprot
Match: A0A7J7CFU2_TRIWF (E3 ubiquitin-protein ligase n=1 Tax=Tripterygium wilfordii TaxID=458696 RepID=A0A7J7CFU2_TRIWF)

HSP 1 Score: 60.8 bits (146), Expect = 3.510e-7
Identity = 26/58 (44.83%), Postives = 37/58 (63.79%), Query Frame = 1 
Query:   40 GSERGSELMMEGADPSCPLCNVAYEEGSDLCLLPCLHVFHENCIHKWCNRHIVCPLCK 213
            G++  S+ ++   D  C +C  AY++G+DL  LPCLH FH  CI KW + +  CPLCK
Sbjct:  312 GTDMPSKRILSEEDTECCICLSAYDDGTDLRELPCLHHFHCTCIDKWLHINATCPLCK 369
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig107685.632.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LID0_ECTSI2.620e-8462.22RING-type domain-containing protein n=1 Tax=Ectoca... [more]
A0A6H5JTX8_9PHAE3.480e-8060.29RING-type domain-containing protein n=1 Tax=Ectoca... [more]
A0A7S0UHT1_9STRA1.680e-943.06Hypothetical protein (Fragment) n=1 Tax=Pseudo-nit... [more]
A0A1S6GLJ1_9POAL8.530e-951.11E3 ubiquitin protein ligase RIE1-like protein (Fra... [more]
A0A0K9P9K8_ZOSMR2.240e-839.19Putative Ring finger protein n=1 Tax=Zostera marin... [more]
A0A383VIV6_TETOB5.410e-843.33RING-type domain-containing protein n=1 Tax=Tetrad... [more]
A0A7I8IBN1_SPIIN9.970e-842.65Hypothetical protein n=1 Tax=Spirodela intermedia ... [more]
A0A7I8JYG6_SPIIN1.840e-747.17Hypothetical protein n=1 Tax=Spirodela intermedia ... [more]
A0A8J5XKQ2_DIALT1.920e-738.57RING-type domain-containing protein n=2 Tax=Diacro... [more]
A0A7J7CFU2_TRIWF3.510e-744.83E3 ubiquitin-protein ligase n=1 Tax=Tripterygium w... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig107685contigH-elongata_contig107685:20..1413 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score286.6
Seed ortholog evalue1.1e-74
Seed eggNOG ortholog2880.D8LID0
Preferred nameZSWIM2
KEGG koko:K15716,ko:K16285
GOsGO:0000209,GO:0003674,GO:0003824,GO:0004842,GO:0005488,GO:0005515,GO:0006464,GO:0006807,GO:0008150,GO:0008152,GO:0009966,GO:0009967,GO:0009987,GO:0010646,GO:0010647,GO:0010941,GO:0010942,GO:0016567,GO:0016740,GO:0019538,GO:0019787,GO:0023051,GO:0023056,GO:0032446,GO:0036211,GO:0042981,GO:0043065,GO:0043067,GO:0043068,GO:0043170,GO:0043412,GO:0043621,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0048518,GO:0048522,GO:0048583,GO:0048584,GO:0050789,GO:0050794,GO:0061630,GO:0061659,GO:0065007,GO:0070647,GO:0071704,GO:0140096,GO:1901564,GO:1902041,GO:1902043,GO:2001233,GO:2001235,GO:2001236,GO:2001238
EggNOG free text desc.ubiquitin-protein transferase activity
EggNOG OGsKOG0800@1,KOG0800@2759
Ec32 ortholog descriptionZinc finger, RING-type
Ec32 orthologEc-18_004170.1
EC2.3.2.27
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko04121
Hectar predicted targeting categoryother localisation
Exons2
Model size717
Cds size717
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622929606.6008615-CDS-H-elongata_contig107685:19..6321622929606.6008615-CDS-H-elongata_contig107685:19..632Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig107685 20..632 -
1691679089.0252712-CDS-H-elongata_contig107685:19..6321691679089.0252712-CDS-H-elongata_contig107685:19..632Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig107685 20..632 -
1622929606.622423-CDS-H-elongata_contig107685:1309..14131622929606.622423-CDS-H-elongata_contig107685:1309..1413Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig107685 1310..1413 -
1691679089.0386188-CDS-H-elongata_contig107685:1309..14131691679089.0386188-CDS-H-elongata_contig107685:1309..1413Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig107685 1310..1413 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig107685.632.1prot_H-elongata_contig107685.632.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig107685 20..1413 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig107685.632.1

>prot_H-elongata_contig107685.632.1 ID=prot_H-elongata_contig107685.632.1|Name=mRNA_H-elongata_contig107685.632.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=239bp
MMFTAVTDDGVSLGSERGSELMMEGADPSCPLCNVAYEEGSDLCLLPCLH
VFHENCIHKWCNRHIVCPLCKRHLEAPEPLHTLPGYGGGMAQSEPVGNGH
DATGRQTWGHMLGTAAPGPASGHGSARGLGGTRSDDGLWNDRVLNAAQRQ
MRGGHNRVTSFSSETRRHNSARGRTGGPPQSSSAGTSVVGRSQSINTSSA
RVHQGFQRTRSSSFSTTVPERGRTLGRVAHGTAPHRGGA
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mRNA from alignment at H-elongata_contig107685:20..1413-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig107685.632.1 ID=mRNA_H-elongata_contig107685.632.1|Name=mRNA_H-elongata_contig107685.632.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1394bp|location=Sequence derived from alignment at H-elongata_contig107685:20..1413- (Himanthalia elongata Himel1 dioecious)
ATGATGTTCACAGCCGTAACGGACGATGGTGTCAGCCTGGGGTCAGAGCG
AGGGTCGGAGCTAATGATGGAGGGAGCGGACCCGTCCTGTCCACTGTGCA
ATGTGTGAGTACTGTTCTTAAAATGCCATGATAGGAGCGAAGGGCACGCG
GTGCGACGTCTCTCCGCTCTTTCGCTAGACAGTCGATCGATTGAGCATTT
CGATTCTGCGGCTGTCTGCACGATTTTGCGTCATCGTCCTTAGTAGACCG
GGGAATGAATGGTGGAAACGCCTTGCGCTTGGCACCCCACTTACCCCCAG
CGCACAAGTTTGTCGCGCCCAGTTGGAGTTATTTTTGGCCTCTTGCTCTA
GGTTTGTTAGGCGCGGTTTCCCCTGTCACGCAGCAGTGGTTGGTTCCATG
CGGCGCGGGATAAAAGTTTTTGTGGTTATTACATATAGCAGTATAGTATA
GTAAGGCCTATAGTATCGTATGGATCAATCGAACATGGTAGCCAACCCTG
TCCCCGTTCGCTTCTGAGAATTTGGTCTCGCGAGACGGGTTTGGCTCTCC
CGTCCCGCGTTAGCCGTAGGTGTTGTTGATCGAGGGAGATTTACGGGGCA
GATACCTAGGCGACATCGTGGTATGTTTCGGAAAGCCCGACCTGCATACC
CTCAGCCGCTCCCATTTCCCCTACCCTTATTTGTCCTCAAAGAAACGAGT
GAAAACCTTCAAACCCTTTTTGCGCATGCCGCGCGTTTCCAAACCCCTGC
TCCCCGATCGACTGCCGCCTGTCGTTGACAGCGCTTACGAGGAGGGGTCC
GACCTGTGCCTTCTCCCTTGTCTCCACGTGTTTCACGAGAACTGCATCCA
CAAATGGTGCAACCGCCACATTGTTTGCCCGCTCTGTAAGCGCCATTTGG
AAGCCCCCGAACCCCTCCACACCCTCCCAGGCTACGGCGGCGGGATGGCT
CAATCCGAACCGGTGGGAAACGGACACGATGCGACCGGTCGCCAGACGTG
GGGCCACATGCTTGGTACCGCGGCGCCGGGACCCGCCAGTGGGCACGGAA
GCGCTAGAGGATTGGGGGGAACTCGGTCCGATGACGGCCTGTGGAACGAT
CGCGTCCTTAACGCTGCACAGCGGCAGATGAGGGGGGGGCATAACAGAGT
GACCTCGTTCAGTTCTGAGACGAGGAGGCACAACAGTGCCCGTGGACGCA
CGGGAGGCCCCCCGCAATCGAGCTCGGCGGGAACGTCCGTCGTCGGCAGG
TCCCAGAGCATTAACACCTCTAGCGCCCGGGTTCACCAAGGCTTTCAAAG
GACGCGCTCGTCGTCTTTCTCTACCACAGTTCCCGAGAGGGGACGGACTC
TGGGGCGCGTCGCGCATGGCACTGCTCCCCATCGTGGGGGCGCG
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Coding sequence (CDS) from alignment at H-elongata_contig107685:20..1413-

>mRNA_H-elongata_contig107685.632.1 ID=mRNA_H-elongata_contig107685.632.1|Name=mRNA_H-elongata_contig107685.632.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=1434bp|location=Sequence derived from alignment at H-elongata_contig107685:20..1413- (Himanthalia elongata Himel1 dioecious)
ATGATGTTCACAGCCGTAACGGACGATGGTGTCAGCCTGGGGTCAGAGCG
AGGGTCGGAGCTAATGATGGAGGGAGCGGACCCGTCCTGTCCACTGTGCA
ATGTATGATGTTCACAGCCGTAACGGACGATGGTGTCAGCCTGGGGTCAG
AGCGAGGGTCGGAGCTAATGATGGAGGGAGCGGACCCGTCCTGTCCACTG
TGCAATGTCGCTTACGAGGAGGGGTCCGACCTGTGCCTTCTCCCTTGTCT
CCACGTGTTTCACGAGAACTGCATCCACAAATGGTGCAACCGCCACATTG
TTTGCCCGCTCTGTAAGCGCCATTTGGAAGCCCCCGAACCCCTCCACACC
CTCCCAGGCTACGGCGGCGGGATGGCTCAATCCGAACCGGTGGGAAACGG
ACACGATGCGACCGGTCGCCAGACGTGGGGCCACATGCTTGGTACCGCGG
CGCCGGGACCCGCCAGTGGGCACGGAAGCGCTAGAGGATTGGGGGGAACT
CGGTCCGATGACGGCCTGTGGAACGATCGCGTCCTTAACGCTGCACAGCG
GCAGATGAGGGGGGGGCATAACAGAGTGACCTCGTTCAGTTCTGAGACGA
GGAGGCACAACAGTGCCCGTGGACGCACGGGAGGCCCCCCGCAATCGAGC
TCGGCGGGAACGTCCGTCGTCGGCAGGTCCCAGAGCATTAACACCTCTAG
CGCCCGGGTTCACCAAGGCTTTCAAAGGACGCGCTCGTCGTCTTTCTCTA
CCACAGTTCCCGAGAGGGGACGGACTCTGGGGCGCGTCGCGCATGGCACT
GCTCCCCATCGTGGGGGCGCGCGCTTACGAGGAGGGGTCCGACCTGTGCC
TTCTCCCTTGTCTCCACGTGTTTCACGAGAACTGCATCCACAAATGGTGC
AACCGCCACATTGTTTGCCCGCTCTGTAAGCGCCATTTGGAAGCCCCCGA
ACCCCTCCACACCCTCCCAGGCTACGGCGGCGGGATGGCTCAATCCGAAC
CGGTGGGAAACGGACACGATGCGACCGGTCGCCAGACGTGGGGCCACATG
CTTGGTACCGCGGCGCCGGGACCCGCCAGTGGGCACGGAAGCGCTAGAGG
ATTGGGGGGAACTCGGTCCGATGACGGCCTGTGGAACGATCGCGTCCTTA
ACGCTGCACAGCGGCAGATGAGGGGGGGGCATAACAGAGTGACCTCGTTC
AGTTCTGAGACGAGGAGGCACAACAGTGCCCGTGGACGCACGGGAGGCCC
CCCGCAATCGAGCTCGGCGGGAACGTCCGTCGTCGGCAGGTCCCAGAGCA
TTAACACCTCTAGCGCCCGGGTTCACCAAGGCTTTCAAAGGACGCGCTCG
TCGTCTTTCTCTACCACAGTTCCCGAGAGGGGACGGACTCTGGGGCGCGT
CGCGCATGGCACTGCTCCCCATCGTGGGGGCGCG
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