mRNA_H-elongata_contig9578.16977.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig9578.16977.1
Unique NamemRNA_H-elongata_contig9578.16977.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig9578.16977.1 vs. uniprot
Match: D7FW63_ECTSI (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FW63_ECTSI)

HSP 1 Score: 228 bits (582), Expect = 3.400e-73
Identity = 111/143 (77.62%), Postives = 125/143 (87.41%), Query Frame = 1 
Query:   25 KKRRVVRRRVPKELVENVALNRAIARLPANYNFEIHKSVWRILEEKAGSVALQFPEGLLMYACVISDILEQFTGCQCVILGDVTYGACCIDDLTATALGCSILIHYGHSCLVPIAVTTLKVLYVFVEVSIDTAHLAASMRETF 453
            +K R VR R+P +++E+ ALNRAI RLPANYNFEIHK+VWRI E+KA +VALQFPEGLLMYACVISDILEQF GCQ VILGDVTYGACCIDDLTA AL C +L+HYGHSCLVPIAVT +KVLYVFVE+SIDT HLA  +R TF
Sbjct:    2 RKHRAVRNRIPADILEDAALNRAIERLPANYNFEIHKTVWRIREDKANNVALQFPEGLLMYACVISDILEQFAGCQSVILGDVTYGACCIDDLTAKALNCDMLVHYGHSCLVPIAVTAVKVLYVFVEISIDTDHLAECIRRTF 144
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BLAST of mRNA_H-elongata_contig9578.16977.1 vs. uniprot
Match: A0A6H5JDJ2_9PHAE (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JDJ2_9PHAE)

HSP 1 Score: 227 bits (579), Expect = 4.030e-71
Identity = 111/143 (77.62%), Postives = 125/143 (87.41%), Query Frame = 1 
Query:   25 KKRRVVRRRVPKELVENVALNRAIARLPANYNFEIHKSVWRILEEKAGSVALQFPEGLLMYACVISDILEQFTGCQCVILGDVTYGACCIDDLTATALGCSILIHYGHSCLVPIAVTTLKVLYVFVEVSIDTAHLAASMRETF 453
            +K+RVVR R+P +++EN ALNRAI RLP NYNFEI K+VWRI E+KA +VALQFPEGLLMYACVISDILEQF GCQ VILGDVTYGACCIDDLTA AL C +L+HYGHSCLVPIAVT +KVLYVFVE+SIDT HLA  +R TF
Sbjct:    2 RKQRVVRNRIPADILENAALNRAIERLPVNYNFEIQKTVWRIREDKANNVALQFPEGLLMYACVISDILEQFAGCQSVILGDVTYGACCIDDLTAKALDCDMLVHYGHSCLVPIAVTAVKVLYVFVEISIDTDHLAECIRRTF 144
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BLAST of mRNA_H-elongata_contig9578.16977.1 vs. uniprot
Match: A0A812U174_SYMMI (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812U174_SYMMI)

HSP 1 Score: 192 bits (487), Expect = 4.450e-57
Identity = 95/154 (61.69%), Postives = 116/154 (75.32%), Query Frame = 1 
Query:    7 NLKVDNKKRRVVRRR---VPKELVENVALNRAIARLPANYNFEIHKSVWRILEEKAGSVALQFPEGLLMYACVISDILEQFTGCQCVILGDVTYGACCIDDLTATALGCSILIHYGHSCLVPIAVTTLKVLYVFVEVSIDTAHLAASMRETFPP 459
            N K  N KR   +RR   +P ++V N ALN AIA LPANYNFEIHK+VWR+  + A  VALQFPEGLLMYAC+I+DILE F   + VI+ DVTYGACCIDD TA  +G  +L+HYGHSCLVP+ VT++KVLYVFVE++ DT+HL A  R +F P
Sbjct:    1 NKKAGNVKRVAPQRRRNQIPDDIVHNEALNSAIALLPANYNFEIHKTVWRVKSDSAKLVALQFPEGLLMYACIIADILENFADTKVVIMADVTYGACCIDDFTAKKIGADLLVHYGHSCLVPVNVTSMKVLYVFVEIAFDTSHLLACFRHSFAP 154
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BLAST of mRNA_H-elongata_contig9578.16977.1 vs. uniprot
Match: A0A1V9ZBQ6_9STRA (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZBQ6_9STRA)

HSP 1 Score: 191 bits (485), Expect = 1.250e-56
Identity = 88/145 (60.69%), Postives = 115/145 (79.31%), Query Frame = 1 
Query:   31 RRVVRRRVPKELVENVALNRAIARLPANYNFEIHKSVWRILEEKAGSVALQFPEGLLMYACVISDILEQFTGCQCVILGDVTYGACCIDDLTATALGCSILIHYGHSCLVPIAVTTLKVLYVFVEVSIDTAHLAASMRETFPPVS 465
            RR +  ++P E+V+N  L +AI  LP NYNFEI K++W+I + ++  VALQFPEGLL+YACVISDI+E+FTG  C+I+GDVTYGACC+DDLTA ALG   ++HYGHSCLVPI VTT+K+LYVFV++SID  H+   M+ TF P +
Sbjct:   38 RRRMANQIPDEIVKNEQLTKAIEELPWNYNFEIRKTIWKIKQAESKRVALQFPEGLLLYACVISDIIERFTGATCIIMGDVTYGACCVDDLTARALGADFMVHYGHSCLVPIDVTTIKMLYVFVDISIDITHMIECMKLTFEPTT 182
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BLAST of mRNA_H-elongata_contig9578.16977.1 vs. uniprot
Match: A0A7S3HKS1_9STRA (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 n=2 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3HKS1_9STRA)

HSP 1 Score: 189 bits (480), Expect = 6.480e-56
Identity = 91/136 (66.91%), Postives = 107/136 (78.68%), Query Frame = 1 
Query:   52 VPKELVENVALNRAIARLPANYNFEIHKSVWRILEEKAGSVALQFPEGLLMYACVISDILEQFTGCQCVILGDVTYGACCIDDLTATALGCSILIHYGHSCLVPIAVTTLKVLYVFVEVSIDTAHLAASMRETFPP 459
            VP  ++ + ALN AIA LP NYNFEIHKSVW++  E  G+VALQFPEGLLMYACVISDI+ +F G + +ILGDVTYGACCIDD TA  LG  +LIHYGHSCLVPI  T +KVLYVFVE+  DT+HL A + + FPP
Sbjct:   40 VPDAVLRDPALNAAIALLPDNYNFEIHKSVWKVKSEYIGTVALQFPEGLLMYACVISDIIARFGGAKVIILGDVTYGACCIDDFTAQKLGAELLIHYGHSCLVPINTTRIKVLYVFVEIQFDTSHLVAVLTKHFPP 175
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BLAST of mRNA_H-elongata_contig9578.16977.1 vs. uniprot
Match: A0A165EKN5_9BASI (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 n=1 Tax=Calocera cornea HHB12733 TaxID=1353952 RepID=A0A165EKN5_9BASI)

HSP 1 Score: 190 bits (483), Expect = 3.270e-55
Identity = 88/135 (65.19%), Postives = 112/135 (82.96%), Query Frame = 1 
Query:   52 VPKELVENVALNRAIARLPANYNFEIHKSVWRILEEKAGSVALQFPEGLLMYACVISDILEQFTGCQCVILGDVTYGACCIDDLTATALGCSILIHYGHSCLVPIAVTTLKVLYVFVEVSIDTAHLAASMRETFP 456
            +P  ++++ ALN AIA+LPANY+FEIHK+VW+I + +A  V LQ PEGLLM+AC+ISDI+E+FTG   VI+GDVTYGACCIDD TA ALGC +L+HYGHSCLVP+  TT+K LYVFVE++ID AHLA ++R  FP
Sbjct:   50 IPSSILQDQALNAAIAQLPANYSFEIHKTVWQIRKNEAKVVGLQMPEGLLMFACLISDIVERFTGALTVIMGDVTYGACCIDDYTAVALGCDMLVHYGHSCLVPVDTTTIKTLYVFVEIAIDNAHLARTVRLNFP 184
[Close]
BLAST of mRNA_H-elongata_contig9578.16977.1 vs. uniprot
Match: A0A6A4Z0R1_9STRA (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A6A4Z0R1_9STRA)

HSP 1 Score: 183 bits (464), Expect = 1.130e-54
Identity = 86/142 (60.56%), Postives = 111/142 (78.17%), Query Frame = 1 
Query:   31 RRVVRRRVPKELVENVALNRAIARLPANYNFEIHKSVWRILEEKAGSVALQFPEGLLMYACVISDILEQFTGCQCVILGDVTYGACCIDDLTATALGCSILIHYGHSCLVPIAVTTLKVLYVFVEVSIDTAHLAASMRETFP 456
            RR +  ++P E+  +  L +AI +LP NYNFEI K++W+I +  A  VALQFPEGLL+YACVISDILE+F G   +I+GDVTYGACC+DDLTA ALG   ++HYGHSCLVPI VTT+K+LYVFV+++ID +HL   M+ TFP
Sbjct:   55 RRKMANQIPDEIQNDPQLTKAIEQLPWNYNFEIRKTIWKIKQAGAKRVALQFPEGLLLYACVISDILERFAGATSIIMGDVTYGACCVDDLTAKALGADFMVHYGHSCLVPIDVTTIKMLYVFVDITIDVSHLVECMKLTFP 196
[Close]
BLAST of mRNA_H-elongata_contig9578.16977.1 vs. uniprot
Match: A0A167GF46_CALVF (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 n=1 Tax=Calocera viscosa (strain TUFC12733) TaxID=1330018 RepID=A0A167GF46_CALVF)

HSP 1 Score: 189 bits (479), Expect = 1.270e-54
Identity = 87/135 (64.44%), Postives = 112/135 (82.96%), Query Frame = 1 
Query:   52 VPKELVENVALNRAIARLPANYNFEIHKSVWRILEEKAGSVALQFPEGLLMYACVISDILEQFTGCQCVILGDVTYGACCIDDLTATALGCSILIHYGHSCLVPIAVTTLKVLYVFVEVSIDTAHLAASMRETFP 456
            +P+ ++++ ALN AIA+LPANY+FEIHK+VW+I + +A  V LQ PEGLLM+AC+ISDI+E+FTG   VI+GDVTYGACCIDD TA ALGC +L+HYGHSCLVP+  TT+K LYVFVE++ID  HLA ++R  FP
Sbjct:   50 IPQSILQDQALNAAIAQLPANYSFEIHKTVWQIRKNEAKMVGLQMPEGLLMFACLISDIVERFTGALTVIMGDVTYGACCIDDYTALALGCDMLVHYGHSCLVPVDTTTIKTLYVFVEIAIDNLHLAKTVRLNFP 184
[Close]
BLAST of mRNA_H-elongata_contig9578.16977.1 vs. uniprot
Match: A0A1Y1VHR7_9FUNG (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 n=1 Tax=Piromyces finnis TaxID=1754191 RepID=A0A1Y1VHR7_9FUNG)

HSP 1 Score: 185 bits (470), Expect = 3.280e-54
Identity = 86/138 (62.32%), Postives = 106/138 (76.81%), Query Frame = 1 
Query:   52 VPKELVENVALNRAIARLPANYNFEIHKSVWRILEEKAGSVALQFPEGLLMYACVISDILEQFTGCQCVILGDVTYGACCIDDLTATALGCSILIHYGHSCLVPIAVTTLKVLYVFVEVSIDTAHLAASMRETFPPVS 465
            +P+E+  N  LN+AI +LP+NYNFEIHK VW+I   +A  VALQFPEGL M+AC ISDILE+FT  + VI+GDVTYGACC+DDLTA ALGC  LIHY HSCLVPI VTT+K +YVFV++ ID  H   +++  F P S
Sbjct:   58 IPEEITNNPLLNKAIQQLPSNYNFEIHKCVWQIKRFEAKKVALQFPEGLQMFACTISDILERFTNVETVIMGDVTYGACCVDDLTAKALGCDFLIHYAHSCLVPITVTTIKTIYVFVDIQIDVQHFVDTIKHNFEPQS 195
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BLAST of mRNA_H-elongata_contig9578.16977.1 vs. uniprot
Match: A0A067CU80_SAPPC (2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 n=3 Tax=Saprolegniaceae TaxID=4764 RepID=A0A067CU80_SAPPC)

HSP 1 Score: 185 bits (469), Expect = 4.730e-54
Identity = 85/143 (59.44%), Postives = 111/143 (77.62%), Query Frame = 1 
Query:   31 RRVVRRRVPKELVENVALNRAIARLPANYNFEIHKSVWRILEEKAGSVALQFPEGLLMYACVISDILEQFTGCQCVILGDVTYGACCIDDLTATALGCSILIHYGHSCLVPIAVTTLKVLYVFVEVSIDTAHLAASMRETFPP 459
            RR +  ++P E+  +  L +AI +LP NYNFEI K++W++ +  +  VALQFPEGLL+YACVISDI+E+F G  C+I+GDVTYGACC+DDLTA ALG   ++HYGHSCLVPI VTT+K+LYVFV++SID  HL   M+ TF P
Sbjct:   55 RRKMANQIPDEIQNDPELGKAIEQLPWNYNFEIRKTIWKLKQANSKRVALQFPEGLLLYACVISDIIERFAGATCIIMGDVTYGACCVDDLTAKALGADFMVHYGHSCLVPIDVTTIKMLYVFVDISIDVTHLIECMKLTFAP 197
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig9578.16977.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FW63_ECTSI3.400e-7377.622-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A6H5JDJ2_9PHAE4.030e-7177.622-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A812U174_SYMMI4.450e-5761.692-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A1V9ZBQ6_9STRA1.250e-5660.692-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A7S3HKS1_9STRA6.480e-5666.912-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A165EKN5_9BASI3.270e-5565.192-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A6A4Z0R1_9STRA1.130e-5460.562-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A167GF46_CALVF1.270e-5464.442-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A1Y1VHR7_9FUNG3.280e-5462.322-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A067CU80_SAPPC4.730e-5459.442-(3-amino-3-carboxypropyl)histidine synthase subu... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig9578contigH-elongata_contig9578:8497..9277 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score229.6
Seed ortholog evalue1e-57
Seed eggNOG ortholog2880.D7FW63
Preferred nameDPH1
KEGG rclassRC00021,RC03180
KEGG koko:K07561
KEGG ReactionR10455
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005829,GO:0006417,GO:0006448,GO:0006464,GO:0006807,GO:0008150,GO:0008152,GO:0008283,GO:0009058,GO:0009889,GO:0009987,GO:0010468,GO:0010556,GO:0010608,GO:0017182,GO:0017183,GO:0018193,GO:0018202,GO:0019222,GO:0019538,GO:0030054,GO:0031323,GO:0031326,GO:0031974,GO:0031981,GO:0032268,GO:0034248,GO:0036211,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044464,GO:0050789,GO:0050794,GO:0051171,GO:0051246,GO:0060255,GO:0065007,GO:0070013,GO:0071704,GO:0080090,GO:1900247,GO:1901564,GO:2000112,GO:2000765
EggNOG free text desc.Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2
EggNOG OGsCOG1736@1,KOG2648@2759
Ec32 ortholog descriptionDiphthamide biosynthesis protein 1, putative
Ec32 orthologEc-01_003550.1
EC2.5.1.108
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03012
Hectar predicted targeting categoryother localisation
Exons2
Model size471
Cds size471
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931351.9748714-CDS-H-elongata_contig9578:8496..86521622931351.9748714-CDS-H-elongata_contig9578:8496..8652Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig9578 8497..8652 +
1691679801.5517485-CDS-H-elongata_contig9578:8496..86521691679801.5517485-CDS-H-elongata_contig9578:8496..8652Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig9578 8497..8652 +
1622931351.995401-CDS-H-elongata_contig9578:8962..92771622931351.995401-CDS-H-elongata_contig9578:8962..9277Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig9578 8963..9277 +
1691679801.5670025-CDS-H-elongata_contig9578:8962..92771691679801.5670025-CDS-H-elongata_contig9578:8962..9277Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig9578 8963..9277 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig9578.16977.1prot_H-elongata_contig9578.16977.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig9578 8497..9277 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig9578.16977.1

>prot_H-elongata_contig9578.16977.1 ID=prot_H-elongata_contig9578.16977.1|Name=mRNA_H-elongata_contig9578.16977.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=157bp
MKNLKVDNKKRRVVRRRVPKELVENVALNRAIARLPANYNFEIHKSVWRI
LEEKAGSVALQFPEGLLMYACVISDILEQFTGCQCVILGDVTYGACCIDD
LTATALGCSILIHYGHSCLVPIAVTTLKVLYVFVEVSIDTAHLAASMRET
FPPVSS*
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mRNA from alignment at H-elongata_contig9578:8497..9277+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig9578.16977.1 ID=mRNA_H-elongata_contig9578.16977.1|Name=mRNA_H-elongata_contig9578.16977.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=781bp|location=Sequence derived from alignment at H-elongata_contig9578:8497..9277+ (Himanthalia elongata Himel1 dioecious)
ATGAAGAATCTCAAAGTGGACAATAAGAAGCGGCGAGTTGTGCGTAGGCG
AGTGCCGAAGGAACTTGTGGAGAATGTCGCACTCAATCGCGCTATTGCAC
GACTGCCCGCCAACTACAACTTCGAGATCCACAAAAGTGTATGGCGGATT
TTAGAGGTAGGAAATAGCGAGATTGTAACCAGCATGATAAATTAGCGGCT
TTTGCGGGCGACTATTTAATAGATCGAAGTACTTCTAACGGCTCTTCAAA
TGAAATATACATCTCCATATTTCAGATCGTTGGAGAAAAAGAGGAAAACT
CAAAATTGCAATGGCGGTTTGACCTCGAGCAAAGGTGGACGTGTCTATAT
AGTGATATATGGTTGCTTGTAGAACGTATTCCTTCCTGACGTAATATATT
CCAATTATTTTGTGCATTTGTTTACCTCTGCTGTCTGGTACACTTTTTTT
CCTGTCTGGGGTCCAGGAGAAGGCGGGAAGCGTAGCTCTACAATTCCCTG
AAGGCCTACTGATGTACGCCTGTGTTATCTCTGATATCCTGGAGCAGTTT
ACTGGGTGTCAGTGCGTTATTCTTGGCGACGTAACATATGGTGCTTGCTG
CATTGACGACCTGACCGCGACGGCGTTGGGCTGCAGCATCCTAATTCACT
ACGGGCACAGTTGCTTGGTGCCAATCGCGGTGACGACATTAAAAGTGTTA
TACGTCTTCGTGGAGGTATCTATCGACACGGCGCATTTGGCAGCGAGTAT
GCGGGAGACCTTCCCTCCGGTAAGCTCATAG
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Coding sequence (CDS) from alignment at H-elongata_contig9578:8497..9277+

>mRNA_H-elongata_contig9578.16977.1 ID=mRNA_H-elongata_contig9578.16977.1|Name=mRNA_H-elongata_contig9578.16977.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=942bp|location=Sequence derived from alignment at H-elongata_contig9578:8497..9277+ (Himanthalia elongata Himel1 dioecious)
ATGAAGAATCTCAAAGTGGACAATAAGAAGCGGCGAGTTGTGCGTAGGCG
AGTGCCGAAGGAACTTGTGGAGAATGTCGCACTCAATCGCGCTATTGCAC
GACTGCCCGCCAACTACAACTTCGAGATCCACAAAAGTGTATGGCGGATT
TTAGAGATGAAGAATCTCAAAGTGGACAATAAGAAGCGGCGAGTTGTGCG
TAGGCGAGTGCCGAAGGAACTTGTGGAGAATGTCGCACTCAATCGCGCTA
TTGCACGACTGCCCGCCAACTACAACTTCGAGATCCACAAAAGTGTATGG
CGGATTTTAGAGGAGAAGGCGGGAAGCGTAGCTCTACAATTCCCTGAAGG
CCTACTGATGTACGCCTGTGTTATCTCTGATATCCTGGAGCAGTTTACTG
GGTGTCAGTGCGTTATTCTTGGCGACGTAACATATGGTGCTTGCTGCATT
GACGACCTGACCGCGACGGCGTTGGGCTGCAGCATCCTAATTCACTACGG
GCACAGTTGCTTGGTGCCAATCGCGGTGACGACATTAAAAGTGTTATACG
TCTTCGTGGAGGTATCTATCGACACGGCGCATTTGGCAGCGAGTATGCGG
GAGACCTTCCCTCCGGTAAGCTCATAGGAGAAGGCGGGAAGCGTAGCTCT
ACAATTCCCTGAAGGCCTACTGATGTACGCCTGTGTTATCTCTGATATCC
TGGAGCAGTTTACTGGGTGTCAGTGCGTTATTCTTGGCGACGTAACATAT
GGTGCTTGCTGCATTGACGACCTGACCGCGACGGCGTTGGGCTGCAGCAT
CCTAATTCACTACGGGCACAGTTGCTTGGTGCCAATCGCGGTGACGACAT
TAAAAGTGTTATACGTCTTCGTGGAGGTATCTATCGACACGGCGCATTTG
GCAGCGAGTATGCGGGAGACCTTCCCTCCGGTAAGCTCATAG
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