mRNA_H-elongata_contig85844.16165.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig85844.16165.1
Unique NamemRNA_H-elongata_contig85844.16165.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig85844.16165.1 vs. uniprot
Match: D7FJT1_ECTSI (Alpha-mannosidase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FJT1_ECTSI)

HSP 1 Score: 212 bits (539), Expect = 3.910e-61
Identity = 99/129 (76.74%), Postives = 110/129 (85.27%), Query Frame = 1 
Query:    1 YGPPEGFCFDQACHDEPIMSDPVMADYNAERRASEFADACQEMARNTRGDDIMLQMGGDFGFENANAWFKNLDSLIEVVNREGRVNVRGREYHVFFSTPDLYTEAKHASGLDWSVKKDDFYPYADCPAS 387
            YGPP GFCFDQAC DEP+M DP+M DYN ERRAS+FADACQE+ RNT GDD+MLQMGGDFGFENA+AWFKNL+ L+  VN+EGRVNV       FFSTP LYTEAKHASGL+ SVKKDDF+PYADCPAS
Sbjct:  226 YGPPVGFCFDQACQDEPMMDDPLMVDYNVERRASDFADACQELGRNTLGDDVMLQMGGDFGFENADAWFKNLEILMAAVNKEGRVNV-------FFSTPQLYTEAKHASGLELSVKKDDFFPYADCPAS 347
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BLAST of mRNA_H-elongata_contig85844.16165.1 vs. uniprot
Match: A0A6H5L421_9PHAE (Alpha-mannosidase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L421_9PHAE)

HSP 1 Score: 201 bits (511), Expect = 2.340e-57
Identity = 95/127 (74.80%), Postives = 105/127 (82.68%), Query Frame = 1 
Query:    1 YGPPEGFCFDQACHDEPIMSDPVMADYNAERRASEFADACQEMARNTRGDDIMLQMGGDFGFENANAWFKNLDSLIEVVNREGRVNVRGREYHVFFSTPDLYTEAKHASGLDWSVKKDDFYPYADCP 381
            YGPP GFCFDQAC DEP+     MADYN ERRAS+FADACQE+ RNT GDD+MLQMGGDFGFENA+AWFKNL+ L+  VN+EGRVNV       FFSTP LYTEAKHASGL+ SVKKDDF+PYADCP
Sbjct:  230 YGPPVGFCFDQACQDEPMXXXXXMADYNVERRASDFADACQELGRNTLGDDVMLQMGGDFGFENADAWFKNLEILMAAVNKEGRVNV-------FFSTPQLYTEAKHASGLELSVKKDDFFPYADCP 349
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BLAST of mRNA_H-elongata_contig85844.16165.1 vs. uniprot
Match: UPI00109BABFD (lysosomal alpha-mannosidase n=1 Tax=Exaiptasia diaphana TaxID=2652724 RepID=UPI00109BABFD)

HSP 1 Score: 136 bits (343), Expect = 8.320e-35
Identity = 61/127 (48.03%), Postives = 83/127 (65.35%), Query Frame = 1 
Query:    1 YGPPEGFCFDQACHDEPIMSDPVMADYNAERRASEFADACQEMARNTRGDDIMLQMGGDFGFENANAWFKNLDSLIEVVNREGRVNVRGREYHVFFSTPDLYTEAKHASGLDWSVKKDDFYPYADCP 381
            Y PP GFCFDQ C D+P+M DP + D N + +  +F +   + A++ + ++IML MG DF +ENAN WFKNLD L++ VN++ RV       H F+STP  Y +  H S L W +K DDF+PYADCP
Sbjct:  237 YNPPPGFCFDQFCKDQPMMXDPNLQDMNVKEKTEDFIEYVCDQAKHYKSNNIMLTMGSDFMYENANLWFKNLDILMKYVNQDSRV-------HAFYSTPTTYLDMVHNSNLTWGLKTDDFFPYADCP 356
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BLAST of mRNA_H-elongata_contig85844.16165.1 vs. uniprot
Match: A0A3P9AJY0_ESOLU (Alpha-mannosidase n=5 Tax=Esox lucius TaxID=8010 RepID=A0A3P9AJY0_ESOLU)

HSP 1 Score: 135 bits (341), Expect = 1.820e-34
Identity = 63/130 (48.46%), Postives = 81/130 (62.31%), Query Frame = 1 
Query:    1 YGPPEGFCFDQACHDEPIMSDPVMADYNAERRASEFADACQEMARNTRGDDIMLQMGGDFGFENANAWFKNLDSLIEVVNREGRVNVRGREYHVFFSTPDLYTEAKHASGLDWSVKKDDFYPYADCPASF 390
            Y PPEGFC+DQ C D PI  DP + DYN E   S+F    QE A+  + + I++ MG DF +ENAN W+KNLD LI  VN++    V G   HV +STP  Y +  H + L W+VK+DDF+PYAD    F
Sbjct:  248 YNPPEGFCWDQLCDDPPIRDDPDLEDYNVEDVVSKFLTVAQEQAKVYKTNHIIMTMGSDFQYENANLWYKNLDKLIRYVNQK---QVNGSNIHVLYSTPSCYLQELHRANLSWTVKRDDFFPYADAAHDF 374
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BLAST of mRNA_H-elongata_contig85844.16165.1 vs. uniprot
Match: A7SFA1_NEMVE (Alpha-mannosidase n=1 Tax=Nematostella vectensis TaxID=45351 RepID=A7SFA1_NEMVE)

HSP 1 Score: 135 bits (340), Expect = 2.490e-34
Identity = 63/127 (49.61%), Postives = 79/127 (62.20%), Query Frame = 1 
Query:    1 YGPPEGFCFDQACHDEPIMSDPVMADYNAERRASEFADACQEMARNTRGDDIMLQMGGDFGFENANAWFKNLDSLIEVVNREGRVNVRGREYHVFFSTPDLYTEAKHASGLDWSVKKDDFYPYADCP 381
            Y PP GFCFDQ C D+PI  DP + D N ++R  EF     E     + ++IM+ MG DF +ENAN W+KNLD LI  VN++ RV         F+STP  Y EA HA+ L W +K DDF+PYADCP
Sbjct:  239 YNPPPGFCFDQFCSDQPIQDDPRLYDENVKQRVEEFIKLACEQGSQYKSNNIMMTMGSDFMYENANLWYKNLDKLIAHVNQDSRVRA-------FYSTPTTYLEALHAANLTWGLKTDDFFPYADCP 358
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BLAST of mRNA_H-elongata_contig85844.16165.1 vs. uniprot
Match: A0A6F9DKU6_9ASCI (Alpha-mannosidase n=1 Tax=Phallusia mammillata TaxID=59560 RepID=A0A6F9DKU6_9ASCI)

HSP 1 Score: 129 bits (325), Expect = 2.610e-32
Identity = 59/130 (45.38%), Postives = 82/130 (63.08%), Query Frame = 1 
Query:    1 YGPPEGFCFDQACHDEPIMSDPVMADYNAERRASEFADACQEMARNTRGDDIMLQMGGDFGFENANAWFKNLDSLIEVVNREGRVNVRGREYHVFFSTPDLYTEAKHASGLDWSVKKDDFYPYADCPASF 390
            YGPP+GFCFD+ C D PIM DP + +YN + + + FADA +  A++ + + IM+ MG DF + NA+ W+KNLD LI+ VN         +   VF+STP  Y  A +    DW++K DDF+PYAD P  F
Sbjct:  233 YGPPKGFCFDELCGDSPIMDDPNLEEYNVDDKVNFFADAARHQAKHFKTNHIMMTMGSDFQYSNAHTWYKNLDKLIKYVN------AANKNITVFYSTPSCYLYALNEVNEDWTIKTDDFFPYADIPHGF 356
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BLAST of mRNA_H-elongata_contig85844.16165.1 vs. uniprot
Match: A0A813TGU4_9BILA (Alpha-mannosidase n=1 Tax=Didymodactylos carnosus TaxID=1234261 RepID=A0A813TGU4_9BILA)

HSP 1 Score: 129 bits (325), Expect = 2.610e-32
Identity = 62/130 (47.69%), Postives = 79/130 (60.77%), Query Frame = 1 
Query:    1 YGPPEGFCFDQACHDEPIMSDPVMADYNAERRASEFADACQEMARNTRGDDIMLQMGGDFGFENANAWFKNLDSLIEVVNREGRVNVRGREYHVFFSTPDLYTEAKHASGLDWSVKKDDFYPYADCPASF 390
            YGPP GFCFD  C D+PIM DP + DYN   + + F +A  +  R    + I++ MG DF FENAN WFKNLD LI  VN +    V G + +VF+STP  Y  A + +G  W+ K DDF+PYA  P  F
Sbjct:  237 YGPPGGFCFDTFCGDQPIMDDPRLHDYNVPEKVNAFINAAHDEVRGFATNHIIMTMGSDFQFENANEWFKNLDKLIRYVNAQ---QVNGSDVNVFYSTPSCYLYALNKAGHTWTTKTDDFFPYAHHPHGF 363
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BLAST of mRNA_H-elongata_contig85844.16165.1 vs. uniprot
Match: UPI0009E2074F (lysosomal alpha-mannosidase-like n=1 Tax=Orbicella faveolata TaxID=48498 RepID=UPI0009E2074F)

HSP 1 Score: 129 bits (325), Expect = 2.670e-32
Identity = 60/127 (47.24%), Postives = 77/127 (60.63%), Query Frame = 1 
Query:    1 YGPPEGFCFDQACHDEPIMSDPVMADYNAERRASEFADACQEMARNTRGDDIMLQMGGDFGFENANAWFKNLDSLIEVVNREGRVNVRGREYHVFFSTPDLYTEAKHASGLDWSVKKDDFYPYADCP 381
            Y PP+GFCFDQ C D PI  DP + D N +    +F       A + +   I+L MG DF +ENAN W+KNLD LI+ VN +GRVN        F+STP +Y +A H +   W +K DDF+PYADCP
Sbjct:  440 YNPPKGFCFDQFCTDPPIQDDPRLFDVNVQETVDKFVAITNAQALHYKTKHILLTMGSDFMYENANLWYKNLDKLIKYVNADGRVNA-------FYSTPTMYLDALHKANQTWEMKTDDFFPYADCP 559
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BLAST of mRNA_H-elongata_contig85844.16165.1 vs. uniprot
Match: UPI0007AD0106 (lysosomal alpha-mannosidase-like n=1 Tax=Sinocyclocheilus grahami TaxID=75366 RepID=UPI0007AD0106)

HSP 1 Score: 120 bits (302), Expect = 2.790e-32
Identity = 55/130 (42.31%), Postives = 79/130 (60.77%), Query Frame = 1 
Query:    1 YGPPEGFCFDQACHDEPIMSDPVMADYNAERRASEFADACQEMARNTRGDDIMLQMGGDFGFENANAWFKNLDSLIEVVNREGRVNVRGREYHVFFSTPDLYTEAKHASGLDWSVKKDDFYPYADCPASF 390
            Y PPEGFC+DQ+C D PI  DP + DYN +    +F +A    A   + + I++ MG DF +ENAN W+KN+D LI+ VN    +  +G + +V +STP  Y +  + + L W +K DDF+PYAD    F
Sbjct:   19 YNPPEGFCWDQSCDDAPIRDDPDLEDYNVDEIVQKFLNASYRQAAFYKTNHIIMTMGSDFQYENANLWYKNMDKLIKYVNA---LQAKGSKVNVLYSTPSCYLQELNLANLTWPLKTDDFFPYADAAHDF 145
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BLAST of mRNA_H-elongata_contig85844.16165.1 vs. uniprot
Match: S4RY66_PETMA (Alpha-mannosidase n=2 Tax=Petromyzon marinus TaxID=7757 RepID=S4RY66_PETMA)

HSP 1 Score: 129 bits (324), Expect = 3.560e-32
Identity = 61/130 (46.92%), Postives = 82/130 (63.08%), Query Frame = 1 
Query:    1 YGPPEGFCFDQACHDEPIMSDPVMADYNAERRASEFADACQEMARNTRGDDIMLQMGGDFGFENANAWFKNLDSLIEVVNREGRVNVRGREYHVFFSTPDLYTEAKHASGLDWSVKKDDFYPYADCPASF 390
            Y PP GFCFDQ C DEPI  DP + +YN + R S+F     + A+  R + I++ MG DF +ENAN WFKNLD LI  VN+   + V G + ++ +STP  Y    + + L W+VK+DDF+PYAD P  F
Sbjct:  228 YNPPPGFCFDQFCDDEPIQDDPSLFNYNVDSRTSDFLKEVAKQAKFYRTNHIIMTMGSDFQYENANLWFKNLDKLITYVNQ---LQVNGSKVNLVYSTPTCYLNELNKANLTWTVKEDDFFPYADGPHQF 354
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig85844.16165.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FJT1_ECTSI3.910e-6176.74Alpha-mannosidase n=1 Tax=Ectocarpus siliculosus T... [more]
A0A6H5L421_9PHAE2.340e-5774.80Alpha-mannosidase n=1 Tax=Ectocarpus sp. CCAP 1310... [more]
UPI00109BABFD8.320e-3548.03lysosomal alpha-mannosidase n=1 Tax=Exaiptasia dia... [more]
A0A3P9AJY0_ESOLU1.820e-3448.46Alpha-mannosidase n=5 Tax=Esox lucius TaxID=8010 R... [more]
A7SFA1_NEMVE2.490e-3449.61Alpha-mannosidase n=1 Tax=Nematostella vectensis T... [more]
A0A6F9DKU6_9ASCI2.610e-3245.38Alpha-mannosidase n=1 Tax=Phallusia mammillata Tax... [more]
A0A813TGU4_9BILA2.610e-3247.69Alpha-mannosidase n=1 Tax=Didymodactylos carnosus ... [more]
UPI0009E2074F2.670e-3247.24lysosomal alpha-mannosidase-like n=1 Tax=Orbicella... [more]
UPI0007AD01062.790e-3242.31lysosomal alpha-mannosidase-like n=1 Tax=Sinocyclo... [more]
S4RY66_PETMA3.560e-3246.92Alpha-mannosidase n=2 Tax=Petromyzon marinus TaxID... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig85844contigH-elongata_contig85844:159..1773 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score216.5
Seed ortholog evalue7.5e-54
Seed eggNOG ortholog2880.D7FJT1
KEGG koko:K01191,ko:K12311
KEGG ReactionR08717,R08718
KEGG Pathwayko00511,ko04142,map00511,map04142
GOsGO:0000323,GO:0001775,GO:0002252,GO:0002263,GO:0002274,GO:0002275,GO:0002283,GO:0002366,GO:0002376,GO:0002443,GO:0002444,GO:0002446,GO:0003674,GO:0003824,GO:0004553,GO:0004559,GO:0005575,GO:0005576,GO:0005615,GO:0005618,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005766,GO:0005773,GO:0005774,GO:0005775,GO:0005975,GO:0005996,GO:0006013,GO:0006464,GO:0006517,GO:0006807,GO:0006810,GO:0006887,GO:0006955,GO:0008150,GO:0008152,GO:0009056,GO:0009100,GO:0009311,GO:0009313,GO:0009505,GO:0009987,GO:0012505,GO:0015923,GO:0016020,GO:0016052,GO:0016192,GO:0016787,GO:0016798,GO:0019318,GO:0019538,GO:0030141,GO:0030312,GO:0031090,GO:0031410,GO:0031974,GO:0031982,GO:0031983,GO:0032940,GO:0034774,GO:0035578,GO:0036211,GO:0036230,GO:0042119,GO:0042582,GO:0043170,GO:0043202,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043299,GO:0043312,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044281,GO:0044421,GO:0044422,GO:0044424,GO:0044433,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0045055,GO:0045321,GO:0046903,GO:0048046,GO:0050896,GO:0051179,GO:0051234,GO:0060205,GO:0070013,GO:0071704,GO:0071944,GO:0097708,GO:0098588,GO:0098805,GO:0099503,GO:1901135,GO:1901564,GO:1901575
EggNOG free text desc.mannose metabolic process
EggNOG OGsKOG1959@1,KOG1959@2759
EC3.2.1.24
COG Functional cat.G
CAZyGH38
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko04131
Hectar predicted targeting categoryother localisation
Exons3
Model size390
Cds size390
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig85844.16165.1prot_H-elongata_contig85844.16165.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig85844 159..1773 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931261.8557613-CDS-H-elongata_contig85844:158..2691622931261.8557613-CDS-H-elongata_contig85844:158..269Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig85844 159..269 -
1691679764.218479-CDS-H-elongata_contig85844:158..2691691679764.218479-CDS-H-elongata_contig85844:158..269Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig85844 159..269 -
1622931261.8858225-CDS-H-elongata_contig85844:885..10411622931261.8858225-CDS-H-elongata_contig85844:885..1041Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig85844 886..1041 -
1691679764.2335572-CDS-H-elongata_contig85844:885..10411691679764.2335572-CDS-H-elongata_contig85844:885..1041Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig85844 886..1041 -
1622931261.918645-CDS-H-elongata_contig85844:1650..17731622931261.918645-CDS-H-elongata_contig85844:1650..1773Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig85844 1651..1773 -
1691679764.2445457-CDS-H-elongata_contig85844:1650..17731691679764.2445457-CDS-H-elongata_contig85844:1650..1773Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig85844 1651..1773 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig85844.16165.1

>prot_H-elongata_contig85844.16165.1 ID=prot_H-elongata_contig85844.16165.1|Name=mRNA_H-elongata_contig85844.16165.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=130bp
YGPPEGFCFDQACHDEPIMSDPVMADYNAERRASEFADACQEMARNTRGD
DIMLQMGGDFGFENANAWFKNLDSLIEVVNREGRVNVRGREYHVFFSTPD
LYTEAKHASGLDWSVKKDDFYPYADCPASF
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mRNA from alignment at H-elongata_contig85844:159..1773-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig85844.16165.1 ID=mRNA_H-elongata_contig85844.16165.1|Name=mRNA_H-elongata_contig85844.16165.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1615bp|location=Sequence derived from alignment at H-elongata_contig85844:159..1773- (Himanthalia elongata Himel1 dioecious)
TACGGCCCGCCGGAAGGGTTCTGCTTCGATCAGGCGTGCCACGATGAGCC
GATCATGAGCGACCCCGTTATGGCCGATTACAACGCGGAGCGCAGGGCCT
CCGAATTCGCCGACGCCTGCCAGGTCAAGAGTAAAACCGGGTGGTTTAGG
AAGAGTCGAGGAGTGGAAGAAGTAGGGCAGAGAAAAAGCACGTTTCATGA
CGGGAAAGTCCAGCTTCTCGCTGTTGCGCACCCCACAAAGTCGCTATTGC
CGAAAAGAGCTGTGGTGGTGCCACATGGACGCATATGCCCTGTGTGCCGC
TCGTTGGTGATATGGGGGCGGTACCAGGCGTACAGAAACACGCGAGGTTG
GGGAAAACTATTGCACAGAACGGATGGGTGGGTGGTTACTGTTGCTGCTG
TTGCCCTCGCTGCTTTTGCGATGGTGGTGTTATGACGCGGAGGTGAGTAG
TTGTATTTTGTCGTTTGTGTGTGCCGATGTGGATGGACATCGACTTGTCT
ATGATGTGTTTTGTGGAAGGACATATTTTTACTAATCCTGTAGAGCTAGT
CACCCATGCAGATGCACACACAAACATAAAAATAAATGAACGCATGTCCC
GCTCGACAGCGCTGTCTCCAAGAGAAGCTTACCGCCCTTCTGGACCTCTA
ACCTTTTCCCCTTTTCCTTGGGAGATGTGCGGTATCTAACATATTTGTGC
TATATACCGTATACCGCATATCATGCGCGCAGGAAATGGCGCGAAACACC
CGCGGGGACGACATCATGCTGCAGATGGGAGGTGACTTCGGCTTCGAGAA
TGCTAACGCTTGGTTCAAGAACCTCGACTCCCTGATCGAAGTCGTGAATC
GAGAGGGTCGCGTCAACGTGAGGGGCCGCGAATACCACGTCCCATCCGCC
CCACTTCCGCCCCACTTGAGCGCTATTACCGTGACCTGTGGTACTGCTTA
GCTAGCGGAGTTTGCCCCCCTGTCCCGTGGCACCTGGCACCAGTATAGTT
TTTGTACAGTATTAATGTGATATCTGCGAATGGTGTCAAATAGACTGTTA
CGTGATACCAAGGCTCGCGGCGATCAGGCGCCGAAGAAGGCGTTAACGAT
CCCTAGTATAAGAGCGCGTCCCACAATCGTCATCGCCATCGCCATAAATC
GGTTTTGCCACCGCTAGGAAAGCATGACATGGTCGACTCGTTGGTCGTTT
TCTGCCGCTGGGATGTCAAAGTTTGTTCAAGTTCAAACTCTGGTGGCTGG
ATGCAACGACGCCTGTATTGGTTTGCATCGTCATATCGTATCATGTCGTA
TCATGTCGTATCATTTCGTATCATATCGTATCACATCGCGTCATGTCATA
TCGCATTATACCGATCTGTGACAGTCGTTTGATGCCTTTTGAGGCACTAC
TTGAGTCATTGCGTAAGCTGGCATTCCCTCATTCGCTTATATCATGTATC
TTGTATCAGCCCCTCCGGCCGGATGAAGTAATTTGACCACAATAATCTTG
GCAGGTGTTCTTCTCCACCCCGGACCTCTACACGGAGGCAAAACACGCTT
CCGGGTTGGACTGGTCTGTTAAGAAAGATGACTTCTACCCGTACGCAGAC
TGCCCGGCGAGTTTC
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Coding sequence (CDS) from alignment at H-elongata_contig85844:159..1773-

>mRNA_H-elongata_contig85844.16165.1 ID=mRNA_H-elongata_contig85844.16165.1|Name=mRNA_H-elongata_contig85844.16165.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=780bp|location=Sequence derived from alignment at H-elongata_contig85844:159..1773- (Himanthalia elongata Himel1 dioecious)
TACGGCCCGCCGGAAGGGTTCTGCTTCGATCAGGCGTGCCACGATGAGCC
GATCATGAGCGACCCCGTTATGGCCGATTACAACGCGGAGCGCAGGGCCT
CCGAATTCGCCGACGCCTGCCAGTACGGCCCGCCGGAAGGGTTCTGCTTC
GATCAGGCGTGCCACGATGAGCCGATCATGAGCGACCCCGTTATGGCCGA
TTACAACGCGGAGCGCAGGGCCTCCGAATTCGCCGACGCCTGCCAGGAAA
TGGCGCGAAACACCCGCGGGGACGACATCATGCTGCAGATGGGAGGTGAC
TTCGGCTTCGAGAATGCTAACGCTTGGTTCAAGAACCTCGACTCCCTGAT
CGAAGTCGTGAATCGAGAGGGTCGCGTCAACGTGAGGGGCCGCGAATACC
ACGAAATGGCGCGAAACACCCGCGGGGACGACATCATGCTGCAGATGGGA
GGTGACTTCGGCTTCGAGAATGCTAACGCTTGGTTCAAGAACCTCGACTC
CCTGATCGAAGTCGTGAATCGAGAGGGTCGCGTCAACGTGAGGGGCCGCG
AATACCACGTGTTCTTCTCCACCCCGGACCTCTACACGGAGGCAAAACAC
GCTTCCGGGTTGGACTGGTCTGTTAAGAAAGATGACTTCTACCCGTACGC
AGACTGCCCGGCGAGTTTCGTGTTCTTCTCCACCCCGGACCTCTACACGG
AGGCAAAACACGCTTCCGGGTTGGACTGGTCTGTTAAGAAAGATGACTTC
TACCCGTACGCAGACTGCCCGGCGAGTTTC
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