mRNA_H-elongata_contig83802.15997.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig83802.15997.1
Unique NamemRNA_H-elongata_contig83802.15997.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig83802.15997.1 vs. uniprot
Match: D8LJA0_ECTSI (Adenylate kinase isoenzyme 6 homolog n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LJA0_ECTSI)

HSP 1 Score: 206 bits (523), Expect = 5.190e-66
Identity = 95/113 (84.07%), Postives = 106/113 (93.81%), Query Frame = 1 
Query:    1 QLCDALEAQMDEGGNVVDYHSCDFFPERWFDLILVLRATTSILFDRLKARGYNEKKVGENVECEIMQVVLDEAREAYPEEAVHEVPSNSVEEMESNVSRVAQWLDAWRKNNPD 339
            +LCDA+E QM+EGGNV+D+HSCDFFPE WFDLILVLRA TS LFDRLKARGY+EKKVGENVECEIMQVVL+EA+E YPEEAVHEVPSN++EE+ESNVSRVAQWLDAWR NN D
Sbjct:   63 KLCDAMETQMEEGGNVIDFHSCDFFPEHWFDLILVLRAETSTLFDRLKARGYSEKKVGENVECEIMQVVLEEAKEGYPEEAVHEVPSNTIEELESNVSRVAQWLDAWRSNNAD 175
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BLAST of mRNA_H-elongata_contig83802.15997.1 vs. uniprot
Match: A0A835YQ97_9STRA (Adenylate kinase isoenzyme 6 homolog n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YQ97_9STRA)

HSP 1 Score: 162 bits (410), Expect = 7.810e-49
Identity = 75/112 (66.96%), Postives = 92/112 (82.14%), Query Frame = 1 
Query:    1 QLCDALEAQMDEGGNVVDYHSCDFFPERWFDLILVLRATTSILFDRLKARGYNEKKVGENVECEIMQVVLDEAREAYPEEAVHEVPSNSVEEMESNVSRVAQWLDAWRKNNP 336
            +LCDAL  +   GG V+D+HSCDFFPE WFDL+LVLRA T+ LFDRLKARGY+EKK+ EN++CEIMQVVL+EA EA+P   +HE+ SN+VEEME+NV RV QWL  WR +NP
Sbjct:   63 KLCDALAPRAAAGGCVLDFHSCDFFPEDWFDLVLVLRADTATLFDRLKARGYHEKKLQENMQCEIMQVVLEEAHEAFPAPIIHELQSNTVEEMEANVDRVKQWLQQWRADNP 174
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BLAST of mRNA_H-elongata_contig83802.15997.1 vs. uniprot
Match: A0A7S2LK93_9STRA (Adenylate kinase isoenzyme 6 homolog n=1 Tax=Leptocylindrus danicus TaxID=163516 RepID=A0A7S2LK93_9STRA)

HSP 1 Score: 158 bits (400), Expect = 4.460e-47
Identity = 73/100 (73.00%), Postives = 85/100 (85.00%), Query Frame = 1 
Query:   40 GNVVDYHSCDFFPERWFDLILVLRATTSILFDRLKARGYNEKKVGENVECEIMQVVLDEAREAYPEEAVHEVPSNSVEEMESNVSRVAQWLDAWRKNNPD 339
            G V D+HSCDFFPERWFDL+LVLRA T +LFDRL  RGY+EKK  EN+ECEIMQ+VLDEARE+Y EE VHEVPSN++E+MESNVSRV QW D W K+N +
Sbjct:   91 GIVTDFHSCDFFPERWFDLVLVLRANTEVLFDRLAERGYSEKKRNENLECEIMQIVLDEARESYAEEIVHEVPSNTLEDMESNVSRVQQWFDHWVKSNDE 190
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BLAST of mRNA_H-elongata_contig83802.15997.1 vs. uniprot
Match: L8H5N4_ACACA (Adenylate kinase isoenzyme 6 homolog n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8H5N4_ACACA)

HSP 1 Score: 155 bits (392), Expect = 4.640e-46
Identity = 71/110 (64.55%), Postives = 89/110 (80.91%), Query Frame = 1 
Query:    1 QLCDALEAQMDEGGNVVDYHSCDFFPERWFDLILVLRATTSILFDRLKARGYNEKKVGENVECEIMQVVLDEAREAYPEEAVHEVPSNSVEEMESNVSRVAQWLDAWRKN 330
            ++CD LE  M  GGNVVD+H+CDFFPERWFDL++VLRA  SIL+ RL +R Y + K+ EN+ECEIMQVVLDEARE+Y +E V E+ +N+VEEMESN SR+ QWL +WR N
Sbjct:   64 KVCDELEDTMTSGGNVVDFHTCDFFPERWFDLVVVLRADNSILYPRLVSRSYKDNKIQENMECEIMQVVLDEARESYKQEIVVELTNNTVEEMESNASRIQQWLASWRSN 173
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BLAST of mRNA_H-elongata_contig83802.15997.1 vs. uniprot
Match: A0A7S2BBB6_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2BBB6_9STRA)

HSP 1 Score: 154 bits (389), Expect = 4.810e-46
Identity = 71/111 (63.96%), Postives = 89/111 (80.18%), Query Frame = 1 
Query:    1 QLCDALEAQMDEGGNVVDYHSCDFFPERWFDLILVLRATTSILFDRLKARGYNEKKVGENVECEIMQVVLDEAREAYPEEAVHEVPSNSVEEMESNVSRVAQWLDAWRKNN 333
            +LCD LE  M EGG VVD+HSCDFFPERWFDL+LVL    + L+DRL  RGY  KKV ENV+CEIMQVVL EA+E+Y EE VH+V S+++++MESNV+RVAQW++ W  +N
Sbjct:   31 KLCDVLEPMMAEGGIVVDFHSCDFFPERWFDLVLVLTCDNTALYDRLVERGYAAKKVEENVQCEIMQVVLQEAKESYAEEVVHQVTSDTIDDMESNVNRVAQWMEQWVADN 141
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BLAST of mRNA_H-elongata_contig83802.15997.1 vs. uniprot
Match: A0A7S2SS55_9STRA (Adenylate kinase isoenzyme 6 homolog n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2SS55_9STRA)

HSP 1 Score: 155 bits (392), Expect = 5.240e-46
Identity = 71/108 (65.74%), Postives = 87/108 (80.56%), Query Frame = 1 
Query:    1 QLCDALEAQMDEGGNVVDYHSCDFFPERWFDLILVLRATTSILFDRLKARGYNEKKVGENVECEIMQVVLDEAREAYPEEAVHEVPSNSVEEMESNVSRVAQWLDAWR 324
            +L DA+E  M++GG VVD+H CD FPERWFDLILVLR   + LFDRL  RGY+  KV ENVECEIMQV+L+EARE++P E VHEV S S+E+M+SNV RVAQWL+ W+
Sbjct:   63 KLLDAMEPLMEQGGQVVDFHGCDLFPERWFDLILVLRCNNTSLFDRLSERGYSATKVSENVECEIMQVILEEARESFPVEIVHEVQSESIEDMDSNVGRVAQWLEQWK 170
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BLAST of mRNA_H-elongata_contig83802.15997.1 vs. uniprot
Match: A0A484E9P9_BRELC (Adenylate kinase isoenzyme 6 homolog n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484E9P9_BRELC)

HSP 1 Score: 156 bits (394), Expect = 5.340e-46
Identity = 69/113 (61.06%), Postives = 89/113 (78.76%), Query Frame = 1 
Query:    1 QLCDALEAQMDEGGNVVDYHSCDFFPERWFDLILVLRATTSILFDRLKARGYNEKKVGENVECEIMQVVLDEAREAYPEEAVHEVPSNSVEEMESNVSRVAQWLDAWRKNNPD 339
            ++CD LE +M EGG VVD+HSCDFFPERWFDL++VLR   + LFDRL+ RGY++KKV ENVECEIM+VVL EAR++Y  E V E+PS +VE+MESN+ RV  W+  W   +P+
Sbjct:   89 KICDELEDRMTEGGQVVDFHSCDFFPERWFDLVVVLRVDNTTLFDRLQKRGYSDKKVAENVECEIMEVVLQEARDSYAPEIVQELPSRTVEDMESNIERVLMWIQHWMSQHPE 201
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BLAST of mRNA_H-elongata_contig83802.15997.1 vs. uniprot
Match: A0A068RTQ9_9FUNG (Adenylate kinase isoenzyme 6 homolog n=2 Tax=Lichtheimia TaxID=688353 RepID=A0A068RTQ9_9FUNG)

HSP 1 Score: 155 bits (392), Expect = 6.280e-46
Identity = 69/111 (62.16%), Postives = 93/111 (83.78%), Query Frame = 1 
Query:    1 QLCDALEAQMDEGGNVVDYHSCDFFPERWFDLILVLRATTSILFDRLKARGYNEKKVGENVECEIMQVVLDEAREAYPEEAVHEVPSNSVEEMESNVSRVAQWLDAWRKNN 333
            +L D +E  M EGG VVD+H+C+ FPERWFDL+LVLRA TS L+DR++ RGYN++K+ EN+ECEIMQVVL+ ARE+Y +E V E+PSN+V++MESNV RV QWL+AW++ +
Sbjct:   75 KLIDEMEDMMQEGGKVVDFHTCEIFPERWFDLVLVLRADTSPLYDRMEKRGYNQRKLQENIECEIMQVVLESARESYAQEIVVELPSNNVDDMESNVDRVKQWLEAWKQQH 185
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BLAST of mRNA_H-elongata_contig83802.15997.1 vs. uniprot
Match: A0A1X2GRL8_9FUNG (Adenylate kinase isoenzyme 6 homolog n=1 Tax=Hesseltinella vesiculosa TaxID=101127 RepID=A0A1X2GRL8_9FUNG)

HSP 1 Score: 154 bits (390), Expect = 9.070e-46
Identity = 70/111 (63.06%), Postives = 91/111 (81.98%), Query Frame = 1 
Query:    1 QLCDALEAQMDEGGNVVDYHSCDFFPERWFDLILVLRATTSILFDRLKARGYNEKKVGENVECEIMQVVLDEAREAYPEEAVHEVPSNSVEEMESNVSRVAQWLDAWRKNN 333
            +L D LE  + +GGNVVDYH+C+ FPERWFDL+LVLR+ T +L+DR+  RGYN+KK+ EN+ CEIMQVVLDEARE+Y  E V E+ SN+V++MESNV RV QWLDAW++ +
Sbjct:   64 KLLDELEDMLTDGGNVVDYHTCELFPERWFDLVLVLRSETHVLYDRMVQRGYNKKKLDENMSCEIMQVVLDEARESYAPEIVVELQSNTVDDMESNVDRVKQWLDAWKEQH 174
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BLAST of mRNA_H-elongata_contig83802.15997.1 vs. uniprot
Match: S2J2C6_MUCC1 (Adenylate kinase isoenzyme 6 homolog n=5 Tax=Mucor TaxID=4830 RepID=S2J2C6_MUCC1)

HSP 1 Score: 154 bits (388), Expect = 1.720e-45
Identity = 70/111 (63.06%), Postives = 90/111 (81.08%), Query Frame = 1 
Query:    1 QLCDALEAQMDEGGNVVDYHSCDFFPERWFDLILVLRATTSILFDRLKARGYNEKKVGENVECEIMQVVLDEAREAYPEEAVHEVPSNSVEEMESNVSRVAQWLDAWRKNN 333
            +L D LE  + +GG VVD+H+C+ FPERWFDL+LVLRA TS L+DR+  RGYN++K+ EN+ECEIMQVVL+ A E+Y  E V E+PSN+V++MESNV RV QWLDAW+ NN
Sbjct:   62 KLLDELEEMLKDGGKVVDFHTCEIFPERWFDLVLVLRADTSSLYDRMVKRGYNKRKIDENMECEIMQVVLETAHESYAPEIVVELPSNTVDDMESNVDRVKQWLDAWKINN 172
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig83802.15997.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LJA0_ECTSI5.190e-6684.07Adenylate kinase isoenzyme 6 homolog n=2 Tax=Ectoc... [more]
A0A835YQ97_9STRA7.810e-4966.96Adenylate kinase isoenzyme 6 homolog n=1 Tax=Tribo... [more]
A0A7S2LK93_9STRA4.460e-4773.00Adenylate kinase isoenzyme 6 homolog n=1 Tax=Lepto... [more]
L8H5N4_ACACA4.640e-4664.55Adenylate kinase isoenzyme 6 homolog n=1 Tax=Acant... [more]
A0A7S2BBB6_9STRA4.810e-4663.96Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A7S2SS55_9STRA5.240e-4665.74Adenylate kinase isoenzyme 6 homolog n=1 Tax=Rhizo... [more]
A0A484E9P9_BRELC5.340e-4661.06Adenylate kinase isoenzyme 6 homolog n=1 Tax=Bremi... [more]
A0A068RTQ9_9FUNG6.280e-4662.16Adenylate kinase isoenzyme 6 homolog n=2 Tax=Licht... [more]
A0A1X2GRL8_9FUNG9.070e-4663.06Adenylate kinase isoenzyme 6 homolog n=1 Tax=Hesse... [more]
S2J2C6_MUCC11.720e-4563.06Adenylate kinase isoenzyme 6 homolog n=5 Tax=Mucor... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig83802contigH-elongata_contig83802:284..1064 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score192.6
Seed ortholog evalue9.5e-47
Seed eggNOG ortholog2880.D8LJA0
Preferred nameFAP7
KEGG rclassRC00002
KEGG koko:K18532
KEGG ReactionR00127,R01547
KEGG Pathwayko00230,ko01100,ko01110,ko01130,ko03008,map00230,map01100,map01110,map01130,map03008
KEGG ModuleM00049
GOsGO:0000166,GO:0000462,GO:0003674,GO:0003824,GO:0004017,GO:0005488,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005829,GO:0006139,GO:0006364,GO:0006396,GO:0006725,GO:0006753,GO:0006793,GO:0006796,GO:0006807,GO:0006950,GO:0006979,GO:0008144,GO:0008150,GO:0008152,GO:0009058,GO:0009117,GO:0009123,GO:0009165,GO:0009987,GO:0010467,GO:0010468,GO:0010494,GO:0015030,GO:0015949,GO:0016070,GO:0016072,GO:0016301,GO:0016310,GO:0016462,GO:0016604,GO:0016740,GO:0016772,GO:0016776,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017076,GO:0017111,GO:0018130,GO:0019205,GO:0019222,GO:0019438,GO:0019637,GO:0022613,GO:0030490,GO:0030554,GO:0031974,GO:0031981,GO:0032553,GO:0032555,GO:0032559,GO:0032991,GO:0033554,GO:0034470,GO:0034599,GO:0034641,GO:0034654,GO:0034660,GO:0035639,GO:0035770,GO:0036094,GO:0036166,GO:0036464,GO:0042221,GO:0042254,GO:0042274,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044085,GO:0044237,GO:0044238,GO:0044249,GO:0044271,GO:0044281,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0046483,GO:0046940,GO:0050145,GO:0050789,GO:0050896,GO:0051716,GO:0055086,GO:0060255,GO:0065007,GO:0070013,GO:0070887,GO:0071704,GO:0071840,GO:0090304,GO:0090407,GO:0097159,GO:0097367,GO:1901265,GO:1901293,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1990904
EggNOG free text desc.adenylate kinase activity
EggNOG OGsCOG1936@1,KOG3347@2759
EC2.7.4.3
COG Functional cat.F
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03009
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionP-loop containing nucleoside triphosphate hydrolase
Ec32 orthologEc-02_005300.1
Exons3
Model size345
Cds size318
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig83802.15997.1prot_H-elongata_contig83802.15997.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig83802 311..1064 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931243.3390598-UTR-H-elongata_contig83802:283..3101622931243.3390598-UTR-H-elongata_contig83802:283..310Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig83802 284..310 +
1691679756.7683337-UTR-H-elongata_contig83802:283..3101691679756.7683337-UTR-H-elongata_contig83802:283..310Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig83802 284..310 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931243.3595657-CDS-H-elongata_contig83802:310..3691622931243.3595657-CDS-H-elongata_contig83802:310..369Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig83802 311..369 +
1691679756.7844875-CDS-H-elongata_contig83802:310..3691691679756.7844875-CDS-H-elongata_contig83802:310..369Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig83802 311..369 +
1622931243.392058-CDS-H-elongata_contig83802:612..6751622931243.392058-CDS-H-elongata_contig83802:612..675Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig83802 613..675 +
1691679756.7967918-CDS-H-elongata_contig83802:612..6751691679756.7967918-CDS-H-elongata_contig83802:612..675Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig83802 613..675 +
1622931243.4090166-CDS-H-elongata_contig83802:868..10641622931243.4090166-CDS-H-elongata_contig83802:868..1064Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig83802 869..1064 +
1691679756.8063152-CDS-H-elongata_contig83802:868..10641691679756.8063152-CDS-H-elongata_contig83802:868..1064Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig83802 869..1064 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig83802.15997.1

>prot_H-elongata_contig83802.15997.1 ID=prot_H-elongata_contig83802.15997.1|Name=mRNA_H-elongata_contig83802.15997.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=106bp
MDEGGNVVDYHSCDFFPERWFDLILVLRATTSILFDRLKARGYNEKKVGE
NVECEIMQVVLDEAREAYPEEAVHEVPSNSVEEMESNVSRVAQWLDAWRK
NNPDV*
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mRNA from alignment at H-elongata_contig83802:284..1064+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig83802.15997.1 ID=mRNA_H-elongata_contig83802.15997.1|Name=mRNA_H-elongata_contig83802.15997.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=781bp|location=Sequence derived from alignment at H-elongata_contig83802:284..1064+ (Himanthalia elongata Himel1 dioecious)
CAGCTCTGCGACGCACTTGAGGCGCAGATGGACGAAGGCGGCAACGTCGT
AGACTACCACTCGTGCGACTTTTTTCCCGAACGCTGGTGAGTCGAAGAGC
TATGGTCCTAATGAACACGTGGATGCCGTCTAAAGCCCTGACCACATGTT
AGCGCATTGGAATCGTTGCTTAGACTATCCTCCGAGCGCTGGAGAATCGA
AGAGCTATGGCCCTAATGAACACGTGGATGCAGTCTAAATCCCTGACCAC
ATGTTAACGCATTGGAATCGGTGCTTAGACTATCCCCGGCAATGGCCGGT
GCGGGGTGAAATCGATGCGCGCTCGACAGGTTCGACTTGATCCTGGTGTT
GAGGGCGACAACGAGCATCCTCTTCGACAGGTTGAAGGCCAGGTATGATC
GCTAAAGCTCGTGTGCGGTTGCTGCCGATTGTTGTTTTTTGGGCGGTCCT
GTGCGAATGACCCGTAAAAAAACTAGAGTACGCTAAAATACAAGGCCTCG
CTGGTACACGCTCCACGTACGATCGCTGTAGACCCCGGTACTGGTGTATG
AGGTAACCCCCCTGATGGATTGTCCTGTTTGCCAGGGGATATAACGAGAA
GAAGGTTGGCGAAAACGTGGAGTGCGAAATCATGCAAGTGGTTCTTGACG
AGGCCCGGGAGGCCTACCCCGAGGAGGCCGTGCACGAGGTCCCCAGCAAC
AGCGTGGAAGAAATGGAGTCGAACGTTTCTAGGGTCGCGCAGTGGCTCGA
TGCTTGGCGAAAAAACAACCCTGATGTTTAA
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Coding sequence (CDS) from alignment at H-elongata_contig83802:284..1064+

>mRNA_H-elongata_contig83802.15997.1 ID=mRNA_H-elongata_contig83802.15997.1|Name=mRNA_H-elongata_contig83802.15997.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=636bp|location=Sequence derived from alignment at H-elongata_contig83802:284..1064+ (Himanthalia elongata Himel1 dioecious)
ATGGACGAAGGCGGCAACGTCGTAGACTACCACTCGTGCGACTTTTTTCC
CGAACGCTGATGGACGAAGGCGGCAACGTCGTAGACTACCACTCGTGCGA
CTTTTTTCCCGAACGCTGGTTCGACTTGATCCTGGTGTTGAGGGCGACAA
CGAGCATCCTCTTCGACAGGTTGAAGGCCAGGTTCGACTTGATCCTGGTG
TTGAGGGCGACAACGAGCATCCTCTTCGACAGGTTGAAGGCCAGGGGATA
TAACGAGAAGAAGGTTGGCGAAAACGTGGAGTGCGAAATCATGCAAGTGG
TTCTTGACGAGGCCCGGGAGGCCTACCCCGAGGAGGCCGTGCACGAGGTC
CCCAGCAACAGCGTGGAAGAAATGGAGTCGAACGTTTCTAGGGTCGCGCA
GTGGCTCGATGCTTGGCGAAAAAACAACCCTGATGTTTAAGGGATATAAC
GAGAAGAAGGTTGGCGAAAACGTGGAGTGCGAAATCATGCAAGTGGTTCT
TGACGAGGCCCGGGAGGCCTACCCCGAGGAGGCCGTGCACGAGGTCCCCA
GCAACAGCGTGGAAGAAATGGAGTCGAACGTTTCTAGGGTCGCGCAGTGG
CTCGATGCTTGGCGAAAAAACAACCCTGATGTTTAA
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