mRNA_H-elongata_contig81961.15862.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig81961.15862.1
Unique NamemRNA_H-elongata_contig81961.15862.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig81961.15862.1 vs. uniprot
Match: D7FUT2_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FUT2_ECTSI)

HSP 1 Score: 174 bits (441), Expect = 5.300e-53
Identity = 97/118 (82.20%), Postives = 99/118 (83.90%), Query Frame = 1 
Query:    4 VNKSMEGVVKGMGTALKSMDVERISKTMDNFEQQFEDMDVRAGYMENAMSDSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGPISNKVPAKPAAETATATEGEDELANRLAALRK 357
            VNKSME            MDVERISKTMD+FEQQFEDMDVRAGYMENAM DSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGP+SNKVPAKPAA  A A   EDELANRLAALRK
Sbjct:   91 VNKSMEXXXXXXXXXXXXMDVERISKTMDSFEQQFEDMDVRAGYMENAMGDSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGPVSNKVPAKPAAAPAAAAGEEDELANRLAALRK 208
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BLAST of mRNA_H-elongata_contig81961.15862.1 vs. uniprot
Match: A0A836CEV0_9STRA (Snf7-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CEV0_9STRA)

HSP 1 Score: 136 bits (342), Expect = 4.700e-38
Identity = 81/121 (66.94%), Postives = 91/121 (75.21%), Query Frame = 1 
Query:    4 VNKSMEGVVKGMGTALKSMDVERISKTMDNFEQQFEDMDVRAGYMENAMSDSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGPISNKVPA---KPAAETATATEGEDELANRLAALRK 357
            +NKSMEGVVKGM +AL SMDVERIS TMD FE+QFEDMDV AGYMEN+M  +TAMSTP +QVD LIQMVADE GLE+G +LDDAG +   VPA   +PA E        D LA RLA LRK
Sbjct:   91 MNKSMEGVVKGMKSALASMDVERISSTMDQFEKQFEDMDVVAGYMENSMGTTTAMSTPPDQVDNLIQMVADEHGLEVGSKLDDAGRVGTTVPAAQQQPAQEV-------DALAQRLADLRK 204
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BLAST of mRNA_H-elongata_contig81961.15862.1 vs. uniprot
Match: A0A836C887_9STRA (Snf7-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C887_9STRA)

HSP 1 Score: 133 bits (335), Expect = 4.940e-37
Identity = 80/119 (67.23%), Postives = 91/119 (76.47%), Query Frame = 1 
Query:    4 VNKSMEGVVKGMGTALKSMDVERISKTMDNFEQQFEDMDVRAGYMENAMSDSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGPISNKVP-AKPAAETATATEGEDELANRLAALRK 357
            V+KSM+GVVKGM  ALK+MDVERISKTMD FE+QFEDMDV AGYMEN+M  +TAMSTP +QVD LIQMVADE GLE+  QLDDAG +   VP A PAA         D+L+ RLA LRK
Sbjct:   91 VDKSMQGVVKGMRKALKTMDVERISKTMDQFEKQFEDMDVVAGYMENSMGATTAMSTPADQVDQLIQMVADEHGLEVASQLDDAGKVGAAVPQAGPAA--------ADDLSQRLADLRK 201
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BLAST of mRNA_H-elongata_contig81961.15862.1 vs. uniprot
Match: A0A7S2H0C6_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2H0C6_9STRA)

HSP 1 Score: 132 bits (331), Expect = 4.020e-36
Identity = 76/117 (64.96%), Postives = 92/117 (78.63%), Query Frame = 1 
Query:    4 VNKSMEGVVKGMGTALKSMDVERISKTMDNFEQQFEDMDVRAGYMENAMSDSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGPISNKVPAKPAAETATATEGEDELANRLAALR 354
            ++ +M GVV+GMG+ALKSMDVE+IS TM +FEQQFEDMDVRAGYME+AM+ STA +TPQE VDGLI+MVADE  L LG  LDDAG + N+ P   A E  +  +G+D LA RLAALR
Sbjct:  113 ISTAMSGVVQGMGSALKSMDVEKISATMTDFEQQFEDMDVRAGYMEDAMNSSTASATPQEDVDGLIKMVADENNLALGSALDDAGAVGNEAPQAKADEKES--QGDD-LAARLAALR 226
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BLAST of mRNA_H-elongata_contig81961.15862.1 vs. uniprot
Match: A0A7S3JWC9_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3JWC9_9STRA)

HSP 1 Score: 127 bits (320), Expect = 9.630e-35
Identity = 72/118 (61.02%), Postives = 92/118 (77.97%), Query Frame = 1 
Query:    4 VNKSMEGVVKGMGTALKSMDVERISKTMDNFEQQFEDMDVRAGYMENAMSDSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGPISNKVPAKPAAETATATEGEDELANRLAALRK 357
            ++K+M  VVKGM +ALKSMDVE+ISKTMD FE+QFEDMDVRAGYMEN+M+ STA +TP++QVD LI  VADE  L+L   LDDAG +S KVP     +    +EG+++LA+RL ALR+
Sbjct:   91 MSKAMGNVVKGMSSALKSMDVEKISKTMDAFEKQFEDMDVRAGYMENSMNASTASATPEDQVDSLISEVADEHQLDLSFALDDAGAVSTKVP-----QEQKQSEGKEDLASRLDALRR 203
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BLAST of mRNA_H-elongata_contig81961.15862.1 vs. uniprot
Match: A0A7S2WTG4_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WTG4_9STRA)

HSP 1 Score: 127 bits (318), Expect = 1.980e-34
Identity = 76/118 (64.41%), Postives = 89/118 (75.42%), Query Frame = 1 
Query:    4 VNKSMEGVVKGMGTALKSMDVERISKTMDNFEQQFEDMDVRAGYMENAMSDSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGPISNKVP-AKPAAETATATEGEDELANRLAALR 354
            +NK+M GVV+GMG+ALKSMDVE+IS TM NFEQQFEDMDVRAGYME AM+ ST+ +TPQE VD LI++VADE  LELG   +DA      VP AKPA    T    +D+LA RLAALR
Sbjct:   91 INKAMVGVVQGMGSALKSMDVEKISATMTNFEQQFEDMDVRAGYMEGAMNSSTSSATPQEDVDSLIKIVADENQLELGAAFEDAA-----VPTAKPAEAEQTEAAPQDDLAARLAALR 203
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BLAST of mRNA_H-elongata_contig81961.15862.1 vs. uniprot
Match: A0A8K1CUU3_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CUU3_PYTOL)

HSP 1 Score: 125 bits (313), Expect = 1.220e-33
Identity = 69/118 (58.47%), Postives = 90/118 (76.27%), Query Frame = 1 
Query:    4 VNKSMEGVVKGMGTALKSMDVERISKTMDNFEQQFEDMDVRAGYMENAMSDSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGPISNKVPAKPAAETATATEGEDELANRLAALRK 357
            + ++M GVVKGM T + SM+VE+IS+TMD FEQQFED+DVRA YME AMS+ T+M+TP +QVD LIQMVADE GL++  QLD AG +  ++P   AAE     + +D+L+ RLAALRK
Sbjct:   94 LKQNMSGVVKGMDTVMASMNVEKISRTMDQFEQQFEDLDVRAAYMEGAMSNGTSMTTPVDQVDDLIQMVADENGLKVAGQLDSAGMVGTELPTAAAAEK----KSQDDLSMRLAALRK 207
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BLAST of mRNA_H-elongata_contig81961.15862.1 vs. uniprot
Match: K3WSW3_GLOUD (Uncharacterized protein n=2 Tax=Pythiaceae TaxID=4782 RepID=K3WSW3_GLOUD)

HSP 1 Score: 124 bits (310), Expect = 3.840e-33
Identity = 65/118 (55.08%), Postives = 89/118 (75.42%), Query Frame = 1 
Query:    4 VNKSMEGVVKGMGTALKSMDVERISKTMDNFEQQFEDMDVRAGYMENAMSDSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGPISNKVPAKPAAETATATEGEDELANRLAALRK 357
            + K+M GVVKGM T + SM+VE+IS+TMD FE+ FEDMDVRA YME AM++ T++STP +QVD LIQMVADE GL++  QLD AG +  ++P+   ++     + +D+L+ RLAALRK
Sbjct:   94 LKKNMNGVVKGMDTVMASMNVEKISQTMDQFEKHFEDMDVRAAYMEGAMNNGTSLSTPADQVDDLIQMVADENGLKIAGQLDSAGLVGTELPSAATSDAVKEKKSQDDLSMRLAALRK 211
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BLAST of mRNA_H-elongata_contig81961.15862.1 vs. uniprot
Match: A0A484DVR9_BRELC (Uncharacterized protein n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484DVR9_BRELC)

HSP 1 Score: 120 bits (301), Expect = 8.280e-32
Identity = 68/115 (59.13%), Postives = 86/115 (74.78%), Query Frame = 1 
Query:   13 SMEGVVKGMGTALKSMDVERISKTMDNFEQQFEDMDVRAGYMENAMSDSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGPISNKVPAKPAAETATATEGEDELANRLAALRK 357
            +M GVV GM   ++SM+VE IS+TMD FE+ FEDMDVR+ YME AMS +T++STP +QVD LIQMVADE GL++  QLD AG +S KVP    AE A   + +D+L+ RLAALRK
Sbjct:   98 NMSGVVYGMDMVMESMNVENISQTMDQFEKHFEDMDVRSAYMEGAMSSNTSLSTPADQVDDLIQMVADENGLKIAGQLDSAGLVSTKVPI---AEAAREKKSQDDLSMRLAALRK 209
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BLAST of mRNA_H-elongata_contig81961.15862.1 vs. uniprot
Match: H3G8P7_PHYRM (Uncharacterized protein n=23 Tax=Peronosporaceae TaxID=4777 RepID=H3G8P7_PHYRM)

HSP 1 Score: 120 bits (300), Expect = 1.170e-31
Identity = 66/115 (57.39%), Postives = 86/115 (74.78%), Query Frame = 1 
Query:   13 SMEGVVKGMGTALKSMDVERISKTMDNFEQQFEDMDVRAGYMENAMSDSTAMSTPQEQVDGLIQMVADEAGLELGEQLDDAGPISNKVPAKPAAETATATEGEDELANRLAALRK 357
            +M GVVKGM T ++SM+VE+IS+TMD FE+ FEDMDVR+ YME AMS  T++STP +QVD LIQMVADE GL++  QLD AG +  ++P     E A   + +D+L+ RLAALRK
Sbjct:   98 NMSGVVKGMDTVMESMNVEKISQTMDQFEKHFEDMDVRSAYMEGAMSSGTSLSTPADQVDDLIQMVADENGLKVAGQLDSAGLVGTELPTT---EAAREKKSQDDLSMRLAALRK 209
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig81961.15862.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FUT2_ECTSI5.300e-5382.20Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A836CEV0_9STRA4.700e-3866.94Snf7-domain-containing protein n=1 Tax=Tribonema m... [more]
A0A836C887_9STRA4.940e-3767.23Snf7-domain-containing protein n=1 Tax=Tribonema m... [more]
A0A7S2H0C6_9STRA4.020e-3664.96Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A7S3JWC9_9STRA9.630e-3561.02Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A7S2WTG4_9STRA1.980e-3464.41Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A8K1CUU3_PYTOL1.220e-3358.47Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
K3WSW3_GLOUD3.840e-3355.08Uncharacterized protein n=2 Tax=Pythiaceae TaxID=4... [more]
A0A484DVR9_BRELC8.280e-3259.13Uncharacterized protein n=1 Tax=Bremia lactucae Ta... [more]
H3G8P7_PHYRM1.170e-3157.39Uncharacterized protein n=23 Tax=Peronosporaceae T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig81961contigH-elongata_contig81961:399..758 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score173.7
Seed ortholog evalue4.9e-41
Seed eggNOG ortholog2880.D7FUT2
Preferred nameCHMP1A
KEGG koko:K12197,ko:K16230
KEGG Pathwayko04144,ko04217,map04144,map04217
KEGG ModuleM00412
GOsGO:0000070,GO:0000228,GO:0000278,GO:0000280,GO:0000793,GO:0000794,GO:0000815,GO:0000819,GO:0000920,GO:0003674,GO:0003824,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005694,GO:0005737,GO:0005768,GO:0005769,GO:0005770,GO:0005815,GO:0005856,GO:0006323,GO:0006355,GO:0006508,GO:0006511,GO:0006605,GO:0006623,GO:0006807,GO:0006810,GO:0006886,GO:0006996,GO:0006997,GO:0007032,GO:0007034,GO:0007049,GO:0007059,GO:0007076,GO:0007080,GO:0007088,GO:0007275,GO:0007346,GO:0007399,GO:0007417,GO:0007420,GO:0008104,GO:0008150,GO:0008152,GO:0008233,GO:0008237,GO:0008270,GO:0009056,GO:0009057,GO:0009889,GO:0009890,GO:0009892,GO:0009966,GO:0009968,GO:0009987,GO:0010008,GO:0010256,GO:0010468,GO:0010556,GO:0010558,GO:0010564,GO:0010605,GO:0010629,GO:0010646,GO:0010648,GO:0010824,GO:0012505,GO:0015031,GO:0015630,GO:0015833,GO:0016020,GO:0016032,GO:0016043,GO:0016050,GO:0016192,GO:0016197,GO:0016363,GO:0016458,GO:0016787,GO:0019058,GO:0019068,GO:0019219,GO:0019222,GO:0019538,GO:0019904,GO:0019941,GO:0021549,GO:0022037,GO:0022402,GO:0022411,GO:0022607,GO:0023051,GO:0023057,GO:0030117,GO:0030163,GO:0030261,GO:0030496,GO:0030902,GO:0031090,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031410,GO:0031974,GO:0031981,GO:0031982,GO:0032501,GO:0032502,GO:0032509,GO:0032511,GO:0032886,GO:0032984,GO:0032991,GO:0033036,GO:0033043,GO:0033365,GO:0034399,GO:0034613,GO:0036257,GO:0036258,GO:0036452,GO:0039702,GO:0042058,GO:0042059,GO:0042802,GO:0042803,GO:0042886,GO:0043162,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043632,GO:0043933,GO:0044085,GO:0044087,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044403,GO:0044419,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044430,GO:0044433,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0045177,GO:0045184,GO:0045324,GO:0045786,GO:0045892,GO:0045934,GO:0046605,GO:0046755,GO:0046872,GO:0046907,GO:0046914,GO:0046983,GO:0048285,GO:0048475,GO:0048513,GO:0048519,GO:0048523,GO:0048583,GO:0048585,GO:0048731,GO:0048856,GO:0050000,GO:0050789,GO:0050794,GO:0051128,GO:0051171,GO:0051172,GO:0051179,GO:0051234,GO:0051252,GO:0051253,GO:0051276,GO:0051301,GO:0051303,GO:0051310,GO:0051493,GO:0051603,GO:0051640,GO:0051641,GO:0051649,GO:0051656,GO:0051704,GO:0051726,GO:0051783,GO:0060236,GO:0060255,GO:0060322,GO:0065007,GO:0070011,GO:0070013,GO:0070507,GO:0070727,GO:0070925,GO:0071103,GO:0071702,GO:0071704,GO:0071705,GO:0071840,GO:0071985,GO:0072594,GO:0072665,GO:0072666,GO:0080090,GO:0090169,GO:0090224,GO:0097708,GO:0098588,GO:0098796,GO:0098805,GO:0098813,GO:0140014,GO:0140096,GO:1901184,GO:1901185,GO:1901564,GO:1901565,GO:1901575,GO:1901673,GO:1902115,GO:1902679,GO:1903047,GO:1903506,GO:1903507,GO:1904896,GO:1904903,GO:2000112,GO:2000113,GO:2001141
EggNOG free text desc.vacuolar transport
EggNOG OGsCOG5491@1,KOG3232@2759
COG Functional cat.N
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03000,ko04131,ko04147
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionSnf7 vacuolar protein sorting protein
Ec32 orthologEc-06_000990.1
Exons1
Model size360
Cds size345
Stop1
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931227.316124-UTR-H-elongata_contig81961:398..4131622931227.316124-UTR-H-elongata_contig81961:398..413Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig81961 399..413 +
1691679750.2687058-UTR-H-elongata_contig81961:398..4131691679750.2687058-UTR-H-elongata_contig81961:398..413Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig81961 399..413 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931227.3397305-CDS-H-elongata_contig81961:413..7581622931227.3397305-CDS-H-elongata_contig81961:413..758Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig81961 414..758 +
1691679750.2795937-CDS-H-elongata_contig81961:413..7581691679750.2795937-CDS-H-elongata_contig81961:413..758Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig81961 414..758 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig81961.15862.1prot_H-elongata_contig81961.15862.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig81961 414..758 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig81961.15862.1

>prot_H-elongata_contig81961.15862.1 ID=prot_H-elongata_contig81961.15862.1|Name=mRNA_H-elongata_contig81961.15862.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=115bp
MEGVVKGMGTALKSMDVERISKTMDNFEQQFEDMDVRAGYMENAMSDSTA
MSTPQEQVDGLIQMVADEAGLELGEQLDDAGPISNKVPAKPAAETATATE
GEDELANRLAALRK*
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mRNA from alignment at H-elongata_contig81961:399..758+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig81961.15862.1 ID=mRNA_H-elongata_contig81961.15862.1|Name=mRNA_H-elongata_contig81961.15862.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=360bp|location=Sequence derived from alignment at H-elongata_contig81961:399..758+ (Himanthalia elongata Himel1 dioecious)
AAGGTCAATAAATCTATGGAGGGCGTCGTGAAAGGCATGGGCACGGCACT
CAAATCGATGGACGTCGAGCGCATCTCGAAAACAATGGACAACTTCGAGC
AGCAGTTCGAAGACATGGACGTACGCGCGGGGTACATGGAGAACGCCATG
AGCGACAGCACGGCGATGTCCACGCCCCAGGAGCAAGTGGACGGGCTCAT
CCAGATGGTTGCGGACGAGGCTGGCTTGGAGCTTGGCGAGCAATTGGACG
ACGCCGGCCCGATATCCAACAAGGTACCCGCGAAGCCCGCTGCGGAAACT
GCCACGGCAACTGAAGGAGAGGATGAGCTTGCGAATAGGCTGGCCGCGCT
GCGCAAGTAG
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Coding sequence (CDS) from alignment at H-elongata_contig81961:399..758+

>mRNA_H-elongata_contig81961.15862.1 ID=mRNA_H-elongata_contig81961.15862.1|Name=mRNA_H-elongata_contig81961.15862.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=690bp|location=Sequence derived from alignment at H-elongata_contig81961:399..758+ (Himanthalia elongata Himel1 dioecious)
ATGGAGGGCGTCGTGAAAGGCATGGGCACGGCACTCAAATCGATGGACGT
CGAGCGCATCTCGAAAACAATGGACAACTTCGAGCAGCAGTTCGAAGACA
TGGACGTACGCGCGGGGTACATGGAGAACGCCATGAGCGACAGCACGGCG
ATGTCCACGCCCCAGGAGCAAGTGGACGGGCTCATCCAGATGGTTGCGGA
CGAGGCTGGCTTGGAGCTTGGCGAGCAATTGGACGACGCCGGCCCGATAT
CCAACAAGGTACCCGCGAAGCCCGCTGCGGAAACTGCCACGGCAACTGAA
GGAGAGGATGAGCTTGCGAATAGGCTGGCCGCGCTGCGCAAGTAGATGGA
GGGCGTCGTGAAAGGCATGGGCACGGCACTCAAATCGATGGACGTCGAGC
GCATCTCGAAAACAATGGACAACTTCGAGCAGCAGTTCGAAGACATGGAC
GTACGCGCGGGGTACATGGAGAACGCCATGAGCGACAGCACGGCGATGTC
CACGCCCCAGGAGCAAGTGGACGGGCTCATCCAGATGGTTGCGGACGAGG
CTGGCTTGGAGCTTGGCGAGCAATTGGACGACGCCGGCCCGATATCCAAC
AAGGTACCCGCGAAGCCCGCTGCGGAAACTGCCACGGCAACTGAAGGAGA
GGATGAGCTTGCGAATAGGCTGGCCGCGCTGCGCAAGTAG
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