mRNA_H-elongata_contig72269.14899.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig72269.14899.1
Unique NamemRNA_H-elongata_contig72269.14899.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig72269.14899.1 vs. uniprot
Match: D8LJD6_ECTSI (Ribosomal protein L11 methyltransferase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LJD6_ECTSI)

HSP 1 Score: 167 bits (423), Expect = 6.310e-48
Identity = 79/104 (75.96%), Postives = 85/104 (81.73%), Query Frame = 1 
Query:    1 QEGWDPIEVGRLRIRFPWHEALPSSQANPSQVELELEGGASFGTGEHPTTSLCLEWLDENSPDQRVLDYGSGSGILSLAALKFGAREAIGVEIDPGAILSAASN 312
            QEGWDPIEVGRLRIRFPWH  LP S+  P QVELELEGG SFGTGEHPTTS+C  WL++N   QRVLDYGSGSGIL LAAL FGA EA+GVE+D  AI SAA N
Sbjct:  212 QEGWDPIEVGRLRIRFPWHTPLPPSELKPGQVELELEGGRSFGTGEHPTTSMCCTWLEDNVAGQRVLDYGSGSGILGLAALSFGAVEAVGVELDLSAIASAAVN 315
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BLAST of mRNA_H-elongata_contig72269.14899.1 vs. uniprot
Match: A0A835Z7L3_9STRA (Ribosomal protein L11 methyltransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z7L3_9STRA)

HSP 1 Score: 139 bits (350), Expect = 1.900e-37
Identity = 67/107 (62.62%), Postives = 79/107 (73.83%), Query Frame = 1 
Query:    1 QEGWDPIEVGRLRIRFPWHEALPSSQANPSQVELELEGGASFGTGEHPTTSLCLEWLD---ENSPDQRVLDYGSGSGILSLAALKFGAREAIGVEIDPGAILSAASN 312
            Q+ W PI +G LRIRFPWH+AL    A   QVEL LEGG +FGTGEHPTT +C  WL+   + SP  RVLDYGSGSG+L LAALK+GA EAIG+E+D  AILS+  N
Sbjct:  186 QQTWHPITLGNLRIRFPWHDALNEDSAGTGQVELCLEGGRAFGTGEHPTTRMCCTWLERCLQVSPGARVLDYGSGSGLLGLAALKYGASEAIGIEVDVDAILSSLYN 292
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BLAST of mRNA_H-elongata_contig72269.14899.1 vs. uniprot
Match: A0A7S1HNT9_HEMAN (Hypothetical protein n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1HNT9_HEMAN)

HSP 1 Score: 115 bits (287), Expect = 2.750e-29
Identity = 62/111 (55.86%), Postives = 75/111 (67.57%), Query Frame = 1 
Query:    1 QEGWDPIEVGRLRIRFPWH--EALPSSQAN----PSQVELELEGGASFGTGEHPTTSLCLEWLDENSPDQ-RVLDYGSGSGILSLAALKFGAREAIGVEIDPGAILSAASN 312
            Q  W PI VG L ++FPWH  E L + +A      S  EL LEGG +FGTGEH TT LC  WL     D  RVLDYG+GSGIL +AAL FGA+EAIGV+ID  ++++AASN
Sbjct:  134 QRNWPPILVGSLLLKFPWHSREELDALRAEMGVGESVPELLLEGGMAFGTGEHATTQLCCNWLQHKVGDGVRVLDYGAGSGILGMAALLFGAKEAIGVDIDRDSLVAAASN 244
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BLAST of mRNA_H-elongata_contig72269.14899.1 vs. uniprot
Match: A0A0G4ERA4_VITBC (Uncharacterized protein n=1 Tax=Vitrella brassicaformis (strain CCMP3155) TaxID=1169540 RepID=A0A0G4ERA4_VITBC)

HSP 1 Score: 114 bits (285), Expect = 6.970e-28
Identity = 55/113 (48.67%), Postives = 72/113 (63.72%), Query Frame = 1 
Query:    1 QEGWDPIEVGRLRIRFPWHEALPSSQANPSQVELELEGGASFGTGEHPTTSLCLEWLD-------ENSPDQ--RVLDYGSGSGILSLAALKFGAREAIGVEIDPGAILSAASN 312
            Q+ W PI +G L IRFPWHE+            L LEGG +FGTG+HPTT LC  W+        E++P +   VLDYGSGSG+L+LAA+ FGA  A+G+EIDP ++ S+  N
Sbjct:  181 QQEWPPIHIGNLTIRFPWHESDTDRGEGDGVKTLVLEGGPAFGTGDHPTTRLCCLWMQKVLREMKEDAPSEPINVLDYGSGSGVLTLAAVLFGASHAVGIEIDPASLTSSRRN 293
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BLAST of mRNA_H-elongata_contig72269.14899.1 vs. uniprot
Match: UPI001BB8F8E2 (50S ribosomal protein L11 methyltransferase n=1 Tax=Sulfuritalea sp. TaxID=2480090 RepID=UPI001BB8F8E2)

HSP 1 Score: 108 bits (270), Expect = 1.310e-26
Identity = 57/106 (53.77%), Postives = 74/106 (69.81%), Query Frame = 1 
Query:    1 QEGWDPIEV-GRLRIRFPWHEALPSSQANPSQVELELEGGASFGTGEHPTTSLCLEWLDEN-SPDQRVLDYGSGSGILSLAALKFGAREAIGVEIDPGAILSAASN 312
            Q  +DPI + GRL I   WH A      +P  V L L+ G +FGTG HPTT LCLEWL+ N +P + VLDYG GSGIL +AAL+ GA++A+GV+IDP A+ +AA+N
Sbjct:  105 QSQFDPIRISGRLWIVPSWHVA-----PDPRAVNLVLDPGLAFGTGSHPTTRLCLEWLEANVAPGRCVLDYGCGSGILGIAALRLGAQDALGVDIDPAALAAAAAN 205
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BLAST of mRNA_H-elongata_contig72269.14899.1 vs. uniprot
Match: A0A1U9JNZ7_9NEIS (Ribosomal protein L11 methyltransferase n=2 Tax=Neisseriales TaxID=206351 RepID=A0A1U9JNZ7_9NEIS)

HSP 1 Score: 107 bits (267), Expect = 3.700e-26
Identity = 56/106 (52.83%), Postives = 71/106 (66.98%), Query Frame = 1 
Query:    1 QEGWDPIEVG-RLRIRFPWHEALPSSQANPSQVELELEGGASFGTGEHPTTSLCLEWLD-ENSPDQRVLDYGSGSGILSLAALKFGAREAIGVEIDPGAILSAASN 312
            Q  +DPI +  RL I   WH+A      NP  + L+L+ G +FGTG HPTT LCL+WLD E SP   VLDYG GSGIL++AALK GA  A+GV+IDP A+ ++  N
Sbjct:  105 QAQFDPIRISDRLWITPTWHDA-----PNPDAINLQLDPGLAFGTGSHPTTRLCLQWLDRELSPGASVLDYGCGSGILAIAALKLGAGRALGVDIDPQAVQASRDN 205
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BLAST of mRNA_H-elongata_contig72269.14899.1 vs. uniprot
Match: UPI000F6132DC (50S ribosomal protein L11 methyltransferase n=1 Tax=Sulfuriferula thiophila TaxID=1781211 RepID=UPI000F6132DC)

HSP 1 Score: 107 bits (266), Expect = 5.290e-26
Identity = 59/106 (55.66%), Postives = 73/106 (68.87%), Query Frame = 1 
Query:    1 QEGWDPIEVG-RLRIRFPWHEALPSSQANPSQVELELEGGASFGTGEHPTTSLCLEWLDENSPDQR-VLDYGSGSGILSLAALKFGAREAIGVEIDPGAILSAASN 312
            Q  +DPI +  RL I   WH+A P  QA    + L+L+ G +FGTG HPTT LCL+WLDEN PD   VLDYG GSGIL++AA KFGA +  GV+IDP AI +A +N
Sbjct:  105 QAEFDPIRISKRLWIIPSWHQA-PDKQA----INLKLDPGLAFGTGSHPTTRLCLQWLDENLPDNATVLDYGCGSGILAIAAKKFGALKVWGVDIDPQAISTAINN 205
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BLAST of mRNA_H-elongata_contig72269.14899.1 vs. uniprot
Match: UPI001EE98601 (50S ribosomal protein L11 methyltransferase n=1 Tax=Kingella sp. CICC 24913 TaxID=2916757 RepID=UPI001EE98601)

HSP 1 Score: 106 bits (265), Expect = 7.430e-26
Identity = 55/106 (51.89%), Postives = 73/106 (68.87%), Query Frame = 1 
Query:    1 QEGWDPIEVG-RLRIRFPWHEALPSSQANPSQVELELEGGASFGTGEHPTTSLCLEWLDEN-SPDQRVLDYGSGSGILSLAALKFGAREAIGVEIDPGAILSAASN 312
            Q  +DPI++  RL I   WH++      +P  + L L+ G +FGTG HPTT LCL WLD+N +  +RVLDYG GSGIL++AALK GA EA GV+IDP A+ +A +N
Sbjct:  105 QSQFDPIQISPRLWITPTWHDS-----PDPEALNLRLDPGLAFGTGSHPTTRLCLRWLDQNLAGGERVLDYGCGSGILAIAALKLGAGEAAGVDIDPQAVKAARAN 205
[Close]
BLAST of mRNA_H-elongata_contig72269.14899.1 vs. uniprot
Match: A0A809RU84_9PROT (Ribosomal protein L11 methyltransferase n=1 Tax=Sulfuriferula nivalis TaxID=2675298 RepID=A0A809RU84_9PROT)

HSP 1 Score: 105 bits (263), Expect = 1.470e-25
Identity = 55/106 (51.89%), Postives = 75/106 (70.75%), Query Frame = 1 
Query:    1 QEGWDPIEVG-RLRIRFPWHEALPSSQANPSQVELELEGGASFGTGEHPTTSLCLEWLDEN-SPDQRVLDYGSGSGILSLAALKFGAREAIGVEIDPGAILSAASN 312
            Q  +DPI++  RL I   WH+A P S+A    + L+L+ G +FGTG HPTT LCL+WLD+N    + VLDYG GSGIL++A++KFGA  A GV+IDP A+++A  N
Sbjct:  105 QSEFDPIQISTRLWIIPSWHDA-PDSKA----INLKLDPGLAFGTGSHPTTRLCLQWLDQNIQGHESVLDYGCGSGILAIASIKFGASSATGVDIDPQAVITALDN 205
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BLAST of mRNA_H-elongata_contig72269.14899.1 vs. uniprot
Match: E7RYE3_9BURK (Ribosomal protein L11 methyltransferase n=2 Tax=Lautropia TaxID=47670 RepID=E7RYE3_9BURK)

HSP 1 Score: 106 bits (264), Expect = 1.610e-25
Identity = 58/106 (54.72%), Postives = 72/106 (67.92%), Query Frame = 1 
Query:    1 QEGWDPIEVG-RLRIRFPWHEALPSSQANPSQVELELEGGASFGTGEHPTTSLCLEWLDENSP-DQRVLDYGSGSGILSLAALKFGAREAIGVEIDPGAILSAASN 312
            Q  +DPI VG RL I   WH A   +QA   +  L ++ G +FGTG HPTT LCLEWLDE+ P   RV+DYG GSGIL++AA + GA E IG++IDP A+LS A N
Sbjct:  130 QAQFDPIPVGQRLLILPSWHAA--ETQAGDREA-LIIDPGLAFGTGSHPTTRLCLEWLDEHDPRGLRVIDYGCGSGILAIAAARLGAAEVIGIDIDPQAVLSTAEN 232
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig72269.14899.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LJD6_ECTSI6.310e-4875.96Ribosomal protein L11 methyltransferase n=2 Tax=Ec... [more]
A0A835Z7L3_9STRA1.900e-3762.62Ribosomal protein L11 methyltransferase n=1 Tax=Tr... [more]
A0A7S1HNT9_HEMAN2.750e-2955.86Hypothetical protein n=1 Tax=Hemiselmis andersenii... [more]
A0A0G4ERA4_VITBC6.970e-2848.67Uncharacterized protein n=1 Tax=Vitrella brassicaf... [more]
UPI001BB8F8E21.310e-2653.7750S ribosomal protein L11 methyltransferase n=1 Ta... [more]
A0A1U9JNZ7_9NEIS3.700e-2652.83Ribosomal protein L11 methyltransferase n=2 Tax=Ne... [more]
UPI000F6132DC5.290e-2655.6650S ribosomal protein L11 methyltransferase n=1 Ta... [more]
UPI001EE986017.430e-2651.8950S ribosomal protein L11 methyltransferase n=1 Ta... [more]
A0A809RU84_9PROT1.470e-2551.89Ribosomal protein L11 methyltransferase n=1 Tax=Su... [more]
E7RYE3_9BURK1.610e-2554.72Ribosomal protein L11 methyltransferase n=2 Tax=La... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig72269contigH-elongata_contig72269:454..2723 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score168.3
Seed ortholog evalue1.9e-39
Seed eggNOG ortholog2880.D8LJD6
KEGG koko:K03301
KEGG TC2.A.12
EggNOG free text desc.Ribosomal protein L11 methyltransferase (PrmA)
EggNOG OGs2QQTZ@2759,COG2264@1
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionribosomal protein L11 methyltransferase
Ec32 orthologEc-17_001920.1
Exons4
Model size312
Cds size312
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig72269.14899.1prot_H-elongata_contig72269.14899.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig72269 454..2723 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931124.194827-CDS-H-elongata_contig72269:453..5041622931124.194827-CDS-H-elongata_contig72269:453..504Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72269 454..504 -
1691679707.984602-CDS-H-elongata_contig72269:453..5041691679707.984602-CDS-H-elongata_contig72269:453..504Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72269 454..504 -
1622931124.2143896-CDS-H-elongata_contig72269:1451..15571622931124.2143896-CDS-H-elongata_contig72269:1451..1557Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72269 1452..1557 -
1691679707.9975147-CDS-H-elongata_contig72269:1451..15571691679707.9975147-CDS-H-elongata_contig72269:1451..1557Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72269 1452..1557 -
1622931124.2305977-CDS-H-elongata_contig72269:2368..24301622931124.2305977-CDS-H-elongata_contig72269:2368..2430Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72269 2369..2430 -
1691679708.0066938-CDS-H-elongata_contig72269:2368..24301691679708.0066938-CDS-H-elongata_contig72269:2368..2430Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72269 2369..2430 -
1622931124.2492468-CDS-H-elongata_contig72269:2630..27231622931124.2492468-CDS-H-elongata_contig72269:2630..2723Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72269 2631..2723 -
1691679708.0160847-CDS-H-elongata_contig72269:2630..27231691679708.0160847-CDS-H-elongata_contig72269:2630..2723Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig72269 2631..2723 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig72269.14899.1

>prot_H-elongata_contig72269.14899.1 ID=prot_H-elongata_contig72269.14899.1|Name=mRNA_H-elongata_contig72269.14899.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=104bp
QEGWDPIEVGRLRIRFPWHEALPSSQANPSQVELELEGGASFGTGEHPTT
SLCLEWLDENSPDQRVLDYGSGSGILSLAALKFGAREAIGVEIDPGAILS
AASN
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mRNA from alignment at H-elongata_contig72269:454..2723-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig72269.14899.1 ID=mRNA_H-elongata_contig72269.14899.1|Name=mRNA_H-elongata_contig72269.14899.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2270bp|location=Sequence derived from alignment at H-elongata_contig72269:454..2723- (Himanthalia elongata Himel1 dioecious)
CAGGAAGGTTGGGATCCCATCGAGGTCGGCCGATTGCGTATTCGATTTCC
GTGGCACGAGGCTCTGCCTTCCTCCCAAGCAAATCCGAGTCAGGTATAAA
TACACGGCTCGCTTGCCGCGCATCGATGGGGGGCGGGGCGGGGCGGGGAG
CGGATTTCCAGAGGGACATTTTTCAGGTGATAGAGAAGGTTATTTATCCA
TTCATTGATCAATCCATCGACTCTTCAAGCTCTGCCCTGGTCCGTCCTAT
GACGTCACGCGGTTACCGTTCGCGCGACGGGTGCACGACACAGGTGGAGC
TCGAGCTCGAGGGTGGAGCAAGCTTTGGGACAGGAGAGCACCCTACGACC
TCCTTGTGCGTTCGTCTCCCCTTCTTCTCTATTTTTCACGCGCGCTCGCA
TGGCGATGACCGCGTGCTCTTTTGATCACCTCGTATATCGAATTGCTTGA
CATTGTGATCGCCGGCAGAGGAGGAGCGCGTCCTCCTCGGTTCGCCCCGG
ATTACCTCTTTGTGCAGGGATGTGGACTATATGGCTATTAATAGTAGATA
ATACGTTTGAAAAGTACCGAGTGAGAGGTTCGCGGCGTGCGCGGTTGTTA
TTCCCTACCCCCCGATAATCCACGCTCCCCGTCCGCGGAGGCTAATTGAC
GGTCATACCACCCGCTGTTTGCGGAGTTTCCCGTCGCCTTTCTTTCTCCG
TTTCATTCAGTAAACGCGCAATACCTAAGTTTTTAGCTTTTAGCTTGTTT
GGTTTTGTGTTTTGTGTGTGTTTTGCGTTGATTGTTATTCCCGTTTTCAT
TGCTATTTCTATTTTTCTTAAAACATACCGTATATGAACTAATTCCACTC
CTCCGAACATCCACACCCTTAGCGTCGTAAGAGGCAGTCCAGGGGACCAA
TTATCGTCAGGTTCGAATGTGTACATAACTACCTTTGAGCACAAAGTCAT
CACACGTGATAAATCGTGTTCCCCAATAGTGAGTTCATCGGGTCAACGAT
TTAATGCTATGATTGTAGACTATATATATATAAGGAGTACCATGCATCCA
TACGGTAAGGAATATTTTTTTGTTGCCTCTTCTTCTGCGGGTATCCCTCA
TGCCTTCCCTCTTTGCTGTCGTTATACACGCTCGTCGTTCCCGTGTCTTG
ATCCGGCGCAGAACAGGTGTCTCGAATGGCTTGACGAGAACTCCCCAGAT
CAGAGAGTCCTGGACTACGGCAGCGGCAGCGGGATACTGAGTCTAGCGGC
GCTTAAGTTCGGCGCAAGAGAGGTACCACACGCTCACGAAATGGCACGCT
GGTAGCACCGTGGGCGGCACATGATATATACACTGCATAGTAAATATAAT
ATATAGCCCGGGGATGACAATCTACCCTTCCCCTCCCATCCCCATCCTCA
TCCCCATTTTTTGTTTTGTTTCTCTCCTTGGGCGACGTCATGAATATCAA
CAAACGTCGCAAGCTTCTGCTGCAGTTTACTTGTGAAGGCTAGCAAAATA
TTAATTTTTTCAAATATCTTGCCGTGTTTCGTTCTGTTCCTGTATCCGTT
CGCACGTTCGTAGCTGCGGAGGCATTCCCAGGGGAGATAGTCATTTCCTA
CTCCCTATCCCCTATCCCCTATGCCCTCTCCTCGTGTGTGTAAAAATATG
TCACGTACGGCAATAAGCATGTACCCAATCGATACCATATTTTTAGAGGC
TTGTGTAGAAACAATCTGGTTTTGGAACCGCAGATTCTTGGCAAGGTTTA
GAGGCGATGTTTTCATGGCTCATGAGGGACCTCAACGACTCGCAAGCAAT
CAGCGGGTGGGGAATGGGGGCGCGAACGGTCGCATGCCAATTACGACATG
TTTTTATCGACTTGAATTTTGAGCATGCATCGACGGCGTCGGGGGGGGGG
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTAGGTTTCCCAATTTTTT
TGGTGCCGTGCCCGTGGGGGGGGGGCTCCGTTTTTGTGGGGGGCCTCGCG
CNNNNNNNNNNGAGGGTTCGTGTGTGTTTGGGTGGGGGGGGGGGGTGGGG
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGCGGGGTGAGGTGT
GTGCTGAGAGGGTATGATTCCCAATATTATTGGTGCCGTGACCGTTGGAG
CGCGCTTCCGTTTCTGATCGAGGCCTCGTGCACCACCGAACTGAACCGAA
CCGAACCGAACCGTCCCAGGCTATAGGCGTCGAGATCGACCCCGGGGCGA
TCTTGAGCGCCGCTAGCAAC
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Coding sequence (CDS) from alignment at H-elongata_contig72269:454..2723-

>mRNA_H-elongata_contig72269.14899.1 ID=mRNA_H-elongata_contig72269.14899.1|Name=mRNA_H-elongata_contig72269.14899.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=624bp|location=Sequence derived from alignment at H-elongata_contig72269:454..2723- (Himanthalia elongata Himel1 dioecious)
CAGGAAGGTTGGGATCCCATCGAGGTCGGCCGATTGCGTATTCGATTTCC
GTGGCACGAGGCTCTGCCTTCCTCCCAAGCAAATCCGAGTCAGCAGGAAG
GTTGGGATCCCATCGAGGTCGGCCGATTGCGTATTCGATTTCCGTGGCAC
GAGGCTCTGCCTTCCTCCCAAGCAAATCCGAGTCAGGTGGAGCTCGAGCT
CGAGGGTGGAGCAAGCTTTGGGACAGGAGAGCACCCTACGACCTCCTTGT
GGAGCTCGAGCTCGAGGGTGGAGCAAGCTTTGGGACAGGAGAGCACCCTA
CGACCTCCTTGTGTCTCGAATGGCTTGACGAGAACTCCCCAGATCAGAGA
GTCCTGGACTACGGCAGCGGCAGCGGGATACTGAGTCTAGCGGCGCTTAA
GTTCGGCGCAAGAGAGGTGTCTCGAATGGCTTGACGAGAACTCCCCAGAT
CAGAGAGTCCTGGACTACGGCAGCGGCAGCGGGATACTGAGTCTAGCGGC
GCTTAAGTTCGGCGCAAGAGAGGCTATAGGCGTCGAGATCGACCCCGGGG
CGATCTTGAGCGCCGCTAGCAACGCTATAGGCGTCGAGATCGACCCCGGG
GCGATCTTGAGCGCCGCTAGCAAC
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