mRNA_H-elongata_contig71493.14810.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig71493.14810.1
Unique NamemRNA_H-elongata_contig71493.14810.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig71493.14810.1 vs. uniprot
Match: D7FJR5_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FJR5_ECTSI)

HSP 1 Score: 243 bits (619), Expect = 2.210e-75
Identity = 130/180 (72.22%), Postives = 154/180 (85.56%), Query Frame = 3 
Query:   15 AEEEGLSALQTVNRRLLAQIEQQGKGIVIPTEDSKKIQRREEDLNGVNPFYAILGAVVAGAMSFGSWKATLWFANAYYSKPAPESDILAVARLTAFLKQAVVGMFALGSGVFGVNALGMGIMAGIVAVGVVKGELDPSPGATASKTAPAGASAEEVDRVMLKEAEEKILRAQADIDRELG 554
            A+ E  + LQTVN RLLAQIE+QGKGIVIPTE+SKKI+R+EEDLNGVNPFYAILGA  A AMSFGSWKATLW A AYYSKPA ESDILAVAR+T+F+KQAVVG+FALG+G+FGV ALGMG+MA IVAVGV KGELDPSP A+ SK A  GAS EE + +ML++A+E + +AQ +I+R +G
Sbjct:   84 ADGETKAKLQTVNARLLAQIEEQGKGIVIPTEESKKIKRKEEDLNGVNPFYAILGAGGAAAMSFGSWKATLWLAQAYYSKPAEESDILAVARMTSFIKQAVVGLFALGAGIFGVTALGMGVMAAIVAVGVAKGELDPSPEASGSKKAAPGASEEEKELLMLQQADESLRKAQEEIERNVG 263
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BLAST of mRNA_H-elongata_contig71493.14810.1 vs. uniprot
Match: A0A835ZBJ7_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZBJ7_9STRA)

HSP 1 Score: 106 bits (264), Expect = 4.860e-23
Identity = 76/188 (40.43%), Postives = 107/188 (56.91%), Query Frame = 3 
Query:    3 AEEDAEE--EGLSALQTVNRRLLAQIEQQ-GKGIVIPTEDSKKIQRREEDLNGVNPFYAILGAVVAGAMSFGSWKATLWFANAYY--SKPAPESDILAVARLTAFLKQAVVGMFALGSGVFGVNALGMGIMAGIVAVGVVKGELDPSPGATASKTAPAGASAEEVDRVMLKEAEEKILRAQADIDREL 551
            A+ D+E+  E  +  Q+VN RLL +I     K  V+       ++R E DLNGV P  A LG+V AGAMS G+W+ T   A  Y   S+    SDILAV RLT  +KQ +VG+  L +GVFGV ALGM ++ G V +GVV GELDPS     + ++     A       L++ ++ + +A A+I  +L
Sbjct:   41 AKPDSEDSTESKAVTQSVNARLLEEINSSMPKYRVLEGATRLSVEREEVDLNGVQPLMAFLGSVGAGAMSAGAWRLTNVLAGIYLVQSEKFANSDILAVQRLTGLMKQVIVGLGTLATGVFGVTALGMALLGGRVLMGVVSGELDPSKVDEKASSSRGRGPAPTAQEGELEDVKQLLAKADAEIAAQL 228
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BLAST of mRNA_H-elongata_contig71493.14810.1 vs. uniprot
Match: A0A7S1UHH8_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1UHH8_9STRA)

HSP 1 Score: 84.3 bits (207), Expect = 1.270e-15
Identity = 46/100 (46.00%), Postives = 64/100 (64.00%), Query Frame = 3 
Query:  120 KIQRREEDLNGVNPFYAILGAVVAGAMSFGSWKATLWFANAYYSKPAPESDILAVARLTAFLKQAVVGMFALGSGVFGVNALGMGIMAGIVAVGVVKGEL 419
            K +R E DLNG NP  A LGA +AG +S  +W+ T +  +AY      ESD     RL+  +KQ VVG+ +L +GVFGV ALG+G++   +AVG+ +GEL
Sbjct:   56 KFERVEVDLNGTNPLQAFLGAGLAGLLSTVAWRFTTFSIDAYAKATPTESDFYLTNRLSGVMKQVVVGLGSLATGVFGVIALGLGLLGARIAVGISRGEL 155
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BLAST of mRNA_H-elongata_contig71493.14810.1 vs. uniprot
Match: A0A7S1D238_CYCTE (Hypothetical protein n=1 Tax=Cyclophora tenuis TaxID=216820 RepID=A0A7S1D238_CYCTE)

HSP 1 Score: 80.1 bits (196), Expect = 8.830e-14
Identity = 58/162 (35.80%), Postives = 85/162 (52.47%), Query Frame = 3 
Query:   21 EEGLSALQTVNRRLLAQIE------------QQGKGIVIPTEDSKKIQRREE-------DLNGVNPFYAILGAVVAGAMSFGSWKATLWFANAYYSKPAPESDILAVARLTAFLKQAVVGMFALGSGVFGVNALGMGIMAGIVAVGVVKGELDPSPGATASK 449
            E+G +A  T+N+RLLA++E            Q  K + +    S K     +       DLNGVNP  A  G V A  M+ G W  T + A AY++     +DI  + R+    +  V+G+ +L SG FGV  +G+ ++AG VA GV+ GELDP+P   A+K
Sbjct:   45 EQGSAATNTINQRLLAELEEATSRETTGRASQLSKNLGLDAFRSNKSDEERQASIEAAKDLNGVNPVVAFAGGVFALGMAAGLWFLTCFLA-AYFADHPANTDIYFIQRVAQVFRNLVMGLVSLASGFFGVIGMGILLLAGRVAYGVMDGELDPTPIKKAAK 205
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BLAST of mRNA_H-elongata_contig71493.14810.1 vs. uniprot
Match: A0A7S2RQ43_9STRA (Hypothetical protein n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RQ43_9STRA)

HSP 1 Score: 73.2 bits (178), Expect = 1.200e-11
Identity = 44/134 (32.84%), Postives = 71/134 (52.99%), Query Frame = 3 
Query:   42 QTVNRRLLAQIEQQGKGIV-----IPTEDSKKIQRREEDLNGVNPFYAILGAVVAGAMSFGSWKATLWFANAYYSKPAPESDILAVARLTAFLKQAVVGMFALGSGVFGVNALGMGIMAGIVAVGVVKGELDPS 428
            Q++N RL+ +I +Q + +      +P  +   I   +ED+  V+PF  + GAV+    S+G WKAT + A  +   P  E     + R    ++   VG F+L SG+FGV A G+  +   V +G++ GEL PS
Sbjct:   50 QSINDRLMMEIAEQEEAVPTLESDLPEMEKAPILGYKEDIEDVDPFLTLGGAVLTAGASYGMWKATGFLATKFAEHPPSEDAFYVIQRAVVLVRTMTVGGFSLASGIFGVTAFGLFALGLRVLIGLLVGELRPS 183
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BLAST of mRNA_H-elongata_contig71493.14810.1 vs. uniprot
Match: A0A7S4B5P2_CHRCT (Hypothetical protein n=1 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4B5P2_CHRCT)

HSP 1 Score: 68.9 bits (167), Expect = 1.260e-9
Identity = 45/126 (35.71%), Postives = 70/126 (55.56%), Query Frame = 3 
Query:   51 NRRLLAQIEQQGKGIVIPTEDSKKIQRREEDLNGVNPFYAILGAVVAGAMSFGSWKATLWFANAYYSKPAPESDILAVARLTAFLKQAVVGMFALGSGVFGVNALGMGIMAGIVAVGVVKGELDPS 428
            N  LLA+I+     ++ PT +    ++   DLNG+ P   ++GA+    +   +W+ T+     +   PA ES    V RLT   +  VV M ALG+GV G+ ++G   +A  VA+GV+KGELDP+
Sbjct:  119 NEALLAEIQ----ALMPPTAEPAP-EKMPVDLNGIRPRDLVIGALSYAVVCVLAWQFTIAAGTYFAEHPAEESSFYVVNRLTGLARVVVVSMGALGAGVTGIASVGQAALAVQVAIGVMKGELDPT 239
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BLAST of mRNA_H-elongata_contig71493.14810.1 vs. uniprot
Match: A0A2C6WEE2_NOSLI (Uncharacterized protein n=2 Tax=Nostoc TaxID=1177 RepID=A0A2C6WEE2_NOSLI)

HSP 1 Score: 54.7 bits (130), Expect = 7.230e-6
Identity = 27/72 (37.50%), Postives = 50/72 (69.44%), Query Frame = 3 
Query:  156 NPFYAILGAVVAGAMSFGSWKATLWFANAYYSKPAPESDILAVARLTAFLKQAVVGMFALGSGVFGVNALGM 371
            +P  +I+GAV++GAM+FG +   +  A ++ +KP    + L + ++T+ ++  VVG+ ALGSG+FG+ A+G+
Sbjct:   19 SPLRSIIGAVISGAMAFGLYSLMISIATSFATKPVHSLNPLVI-KITSAVRTLVVGVVALGSGIFGIVAIGL 89
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig71493.14810.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
D7FJR5_ECTSI2.210e-7572.22Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A835ZBJ7_9STRA4.860e-2340.43Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A7S1UHH8_9STRA1.270e-1546.00Hypothetical protein n=1 Tax=Phaeomonas parva TaxI... [more]
A0A7S1D238_CYCTE8.830e-1435.80Hypothetical protein n=1 Tax=Cyclophora tenuis Tax... [more]
A0A7S2RQ43_9STRA1.200e-1132.84Hypothetical protein n=2 Tax=Rhizochromulina marin... [more]
A0A7S4B5P2_CHRCT1.260e-935.71Hypothetical protein n=1 Tax=Chrysotila carterae T... [more]
A0A2C6WEE2_NOSLI7.230e-637.50Uncharacterized protein n=2 Tax=Nostoc TaxID=1177 ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig71493contigH-elongata_contig71493:437..3106 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score163.7
Seed ortholog evalue5.6e-38
Seed eggNOG ortholog2880.D7FJR5
EggNOG free text desc.Protein of unknown function (DUF3082)
EggNOG OGs2E92Q@1,2SFGP@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionProtein of unknown function DUF3082
Ec32 orthologEc-06_010480.1
Exons5
Model size1091
Cds size375
Stop1
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931115.6064625-UTR-H-elongata_contig71493:436..9551622931115.6064625-UTR-H-elongata_contig71493:436..955Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig71493 437..955 -
1691679704.5305614-UTR-H-elongata_contig71493:436..9551691679704.5305614-UTR-H-elongata_contig71493:436..955Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig71493 437..955 -
1622931115.6842546-UTR-H-elongata_contig71493:2489..26031622931115.6842546-UTR-H-elongata_contig71493:2489..2603Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig71493 2490..2603 -
1691679704.6031413-UTR-H-elongata_contig71493:2489..26031691679704.6031413-UTR-H-elongata_contig71493:2489..2603Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig71493 2490..2603 -
1622931115.6960688-UTR-H-elongata_contig71493:3023..31061622931115.6960688-UTR-H-elongata_contig71493:3023..3106Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig71493 3024..3106 -
1691679704.6134083-UTR-H-elongata_contig71493:3023..31061691679704.6134083-UTR-H-elongata_contig71493:3023..3106Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig71493 3024..3106 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931115.6275566-CDS-H-elongata_contig71493:955..10931622931115.6275566-CDS-H-elongata_contig71493:955..1093Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig71493 956..1093 -
1691679704.54194-CDS-H-elongata_contig71493:955..10931691679704.54194-CDS-H-elongata_contig71493:955..1093Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig71493 956..1093 -
1622931115.6401105-CDS-H-elongata_contig71493:1516..16391622931115.6401105-CDS-H-elongata_contig71493:1516..1639Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig71493 1517..1639 -
1691679704.5516098-CDS-H-elongata_contig71493:1516..16391691679704.5516098-CDS-H-elongata_contig71493:1516..1639Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig71493 1517..1639 -
1622931115.6526597-CDS-H-elongata_contig71493:1812..19051622931115.6526597-CDS-H-elongata_contig71493:1812..1905Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig71493 1813..1905 -
1691679704.5638974-CDS-H-elongata_contig71493:1812..19051691679704.5638974-CDS-H-elongata_contig71493:1812..1905Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig71493 1813..1905 -
1622931115.6712086-CDS-H-elongata_contig71493:2468..24891622931115.6712086-CDS-H-elongata_contig71493:2468..2489Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig71493 2469..2489 -
1691679704.5845764-CDS-H-elongata_contig71493:2468..24891691679704.5845764-CDS-H-elongata_contig71493:2468..2489Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig71493 2469..2489 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig71493.14810.1prot_H-elongata_contig71493.14810.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig71493 956..2489 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig71493.14810.1

>prot_H-elongata_contig71493.14810.1 ID=prot_H-elongata_contig71493.14810.1|Name=mRNA_H-elongata_contig71493.14810.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=125bp
MSFGSWKATLWFANAYYSKPAPESDILAVARLTAFLKQAVVGMFALGSGV
FGVNALGMGIMAGIVAVGVVKGELDPSPGATASKTAPAGASAEEVDRVML
KEAEEKILRAQADIDRELGGKTRR*
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mRNA from alignment at H-elongata_contig71493:437..3106-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig71493.14810.1 ID=mRNA_H-elongata_contig71493.14810.1|Name=mRNA_H-elongata_contig71493.14810.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2670bp|location=Sequence derived from alignment at H-elongata_contig71493:437..3106- (Himanthalia elongata Himel1 dioecious)
AGGCGGAGGAGGACGCAGAGGAGGAAGGATTATCAGCCCTCCAGACAGTA
AACAGGCGCTTGCTTGCCCAGATCGAACAACAGGTCCGTTTATTACCGGT
GCATCGGCTAGAGCACATATGGAAGGTTGACGTGAAACTTCTGCAAGAGA
GCACACGAGGGGAGATGCGATTTGTTCGCAGAAGTCCCCCAATGCTACCT
CGCTCACATTTGTCCCGTTGTGGTTTCTTCCACCTTTGTCCCTCCGTAAA
GTGTACCGGGATGTACCGGGATGTACCGGGATCAAAAGAGAGAGATTTCG
GATGGCAGAGTTTAGCAGTGTTTATCAGTAACAAGCACGCGCTAGAGATA
AAATATCTTTAAAACTATCTTCGCGCGTCTTATCCATTTTAACTTTGTGG
CGGATGCGGCACCTCTGACCGCTGAGCCCGCCCTTTTTTGCGGGGACAAT
TTGATGCCTGGGCATCTCCCATTTTGTACGCGGGCGCTGGCGTCGGGCCG
CAGGGGAAGGGTATCGTTATCCCTACTGAAGACAGCAAGAAAATCCAGCG
ACGAGAGGAGGATTTGAACGGAGTGAACCCCTTCTATGCTATCCTGGGGG
CTGTAGTTGCGGGGGCGATGTCGTTCGGGTCCTGGAAGGTAAACGACAAG
TCTGCTGCATACCTACTTCCACAGGAAATCGAATGCGCCGTCTCACCGTC
TAGCTCGATTCACGCGTGACCCTTCGAGAAAACTCTGCGTCTCGATCCTT
CGTTTCTGCCGGGTCTACAATCGCTGTGCTCAAACAAAGGTACGGGCTGT
GATCAAACAAATGTACGGGCTGTGATCAAAGAAAGGTACGGACTGTGATC
AAAGAAAGGTACGGACTGTGATCAAAGAAAGGTATGGACTGTGATCAAAT
CGACAAAGGAGTCGGTCTTTTTTTAGTTTTGTACCCTCCTTTTGCCACGA
TTGCCACGGTTTTGTTTTAAAAAAATGAACAACTGCTGCTGTAGAAGCTT
TTTCAAAACAGCTGGAAAATCTATGCCACGTTCTCTTAACATGATAATCT
CGCCTTCAGCAATGCGGAGTATAAAGGGAAGGACGGGGGGAAAATTCCGG
TGACCACCGGTTGAAGCTGCAAACGGGAATCGAGAGGGTGTAACGTTCGA
GACTTTTCCCTTCCCCCCCTTCCGATAATGCTAATATCTCTGTCCCCCCA
GGCAACCCTCTGGTTTGCTAACGCATACTACAGCAAACCGGCGCCTGAGA
GTGACATATTGGCCGTGGCACGGCTGACGGCGTTTCTCAAGCAGGTGAGA
AGCGAAGCCATAAGGATTTTATTTTTTTGAAAGCTTCCATGGGATTCTTC
ACGAGGATGGTGGCTGCGCAAAACCTTTGTGAAAGATGCGCAACTCGCCT
TGCCGGTGTTGTCCTTGAAAACGCGAGAAAAATACATTTCCGCGAAACGC
CTACTTCGGTTTTCCAGGCGGTGGTGGGAATGTTCGCCTTGGGGTCTGGT
GTCTTTGGCGTCAACGCCCTTGGCATGGGAATCATGGCGGGAATCGTCGC
CGTGGGCGTCGTCAAGGGTGAACTGGATCCTTCACCCGGGGTACGTACCA
GCCCCACGCAACCTTAGCAACGGATTGCGTCGTTCCTAAATTACTTCATA
CGAAGTACGTATATTTTTCTTCGTATATATCCTCACTCGATTCTCGGTTG
GCGGTCGTTAGGTGACGTGACGTGTTTCACGAAAGGTTTCGAAGTTATGC
CTTGACGGCTGGACTATATTCATTCGCGGGCTGCATAGCTCTTGATCATT
TATCTGTGAACTGGCATCGTGCAACGCCTCGATTAATGTCGAACTGGACA
TTCAACCACGTCGAGGTAAATCGAGGTGCGTTGAGGTGCGCACGACCAGT
TCGTTTCTCCTATTAATAGAGTGGATTCCCCGATCCTATTAATATCATAA
TATTAATATCATAAGAGAACCAATTGCTAAACTGTAGCCTCCACGTTTTT
TTTATGTTTCTAGGCGACGGCATCGAAAACTGCTCCCGCTGGCGCTTCTG
CTGAGGAGGTGGATCGTGTGATGCTCAAGGAGGCCGAAGAGAAAATTCTC
AGGGCTCAGGCGGACATCGACCGTGAACTCGGCGGTAAAACTCGGCGGTA
ATATTTAGCATCCTGACAGCATATGAATCAAGGTGGAACAACCTCTAGGC
GTCAAGCTTTTCAGTATTCCACATTGGAGGAGAGATTGTTGTACTCAGAG
GAGTGCCGAATCGGTGACGGGGTTAGCTTAAGCGGCTAGCGGCTAGCGGC
CAGCCATTGATGCGATGCATAAATCGATTTGAGGCATGGGTGTTGAGGGG
CTTGGAAGCCGACACTATTAATAAACACTTGTATTGATTATGCGGATACC
GAGTATAAACACTTGCAGACGACAAACACTTGCAGACGACGACTCTAAAA
CTTGCGTCTAAAATCATTGGGAATCTGGGAAGTGTGTAGTGTAATTACAT
CGCGTTCCACCTGAAGTATTTGGTACACCTAAAACGTATAATGTAGTAGC
ACTGACTGGACTACCAATATACCAACATATATTATGAAAACACACCTCTG
TGTGAACAATGCCTTAAGCAGCTAATGGCGAGCGAGACGATCTTACGAAA
TACTTCAAATAATAACATGG
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Coding sequence (CDS) from alignment at H-elongata_contig71493:437..3106-

>mRNA_H-elongata_contig71493.14810.1 ID=mRNA_H-elongata_contig71493.14810.1|Name=mRNA_H-elongata_contig71493.14810.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=750bp|location=Sequence derived from alignment at H-elongata_contig71493:437..3106- (Himanthalia elongata Himel1 dioecious)
ATGTCGTTCGGGTCCTGGAAGATGTCGTTCGGGTCCTGGAAGGCAACCCT
CTGGTTTGCTAACGCATACTACAGCAAACCGGCGCCTGAGAGTGACATAT
TGGCCGTGGCACGGCTGACGGCGTTTCTCAAGCAGGCAACCCTCTGGTTT
GCTAACGCATACTACAGCAAACCGGCGCCTGAGAGTGACATATTGGCCGT
GGCACGGCTGACGGCGTTTCTCAAGCAGGCGGTGGTGGGAATGTTCGCCT
TGGGGTCTGGTGTCTTTGGCGTCAACGCCCTTGGCATGGGAATCATGGCG
GGAATCGTCGCCGTGGGCGTCGTCAAGGGTGAACTGGATCCTTCACCCGG
GGCGGTGGTGGGAATGTTCGCCTTGGGGTCTGGTGTCTTTGGCGTCAACG
CCCTTGGCATGGGAATCATGGCGGGAATCGTCGCCGTGGGCGTCGTCAAG
GGTGAACTGGATCCTTCACCCGGGGCGACGGCATCGAAAACTGCTCCCGC
TGGCGCTTCTGCTGAGGAGGTGGATCGTGTGATGCTCAAGGAGGCCGAAG
AGAAAATTCTCAGGGCTCAGGCGGACATCGACCGTGAACTCGGCGGTAAA
ACTCGGCGGTAAGCGACGGCATCGAAAACTGCTCCCGCTGGCGCTTCTGC
TGAGGAGGTGGATCGTGTGATGCTCAAGGAGGCCGAAGAGAAAATTCTCA
GGGCTCAGGCGGACATCGACCGTGAACTCGGCGGTAAAACTCGGCGGTAA
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