mRNA_H-elongata_contig70949.14754.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig70949.14754.1
Unique NamemRNA_H-elongata_contig70949.14754.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig70949.14754.1 vs. uniprot
Match: D8LIP0_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LIP0_ECTSI)

HSP 1 Score: 191 bits (484), Expect = 5.440e-56
Identity = 94/103 (91.26%), Postives = 101/103 (98.06%), Query Frame = 1 
Query:    1 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDITGDGTTSAVLFTGELLRQAERYLSEGLHPRVLTEGFELAKEHTLKLLEAFRSSR 309
            GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDD+TGDGTT++VLFTGELLRQAERYL+EGLHPRVLTEGFELAKEH L+LL+ FRS+R
Sbjct:   40 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDVTGDGTTTSVLFTGELLRQAERYLTEGLHPRVLTEGFELAKEHALELLDTFRSTR 142
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BLAST of mRNA_H-elongata_contig70949.14754.1 vs. uniprot
Match: A0A418EL66_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A418EL66_9STRA)

HSP 1 Score: 167 bits (424), Expect = 2.570e-50
Identity = 81/97 (83.51%), Postives = 92/97 (94.85%), Query Frame = 1 
Query:    1 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDITGDGTTSAVLFTGELLRQAERYLSEGLHPRVLTEGFELAKEHTLKLLE 291
            GPRGTLKMLVGGAGQIKLTKDG VLLHEMQIQHPTA +IAR+ATAQDDITGDGTTS+VLFTGELL+QAER+LS+GLHPR+L EGFELA+E  L++L+
Sbjct:   39 GPRGTLKMLVGGAGQIKLTKDGNVLLHEMQIQHPTAAVIARAATAQDDITGDGTTSSVLFTGELLKQAERFLSDGLHPRILAEGFELAREEALRVLD 135
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BLAST of mRNA_H-elongata_contig70949.14754.1 vs. uniprot
Match: A0A7R9YWJ8_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pseudictyota dubia TaxID=2749911 RepID=A0A7R9YWJ8_9STRA)

HSP 1 Score: 165 bits (417), Expect = 3.410e-50
Identity = 79/100 (79.00%), Postives = 91/100 (91.00%), Query Frame = 1 
Query:    1 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDITGDGTTSAVLFTGELLRQAERYLSEGLHPRVLTEGFELAKEHTLKLLEAFR 300
            GPRGTLKMLVGGAGQIKLTKDG VLLHEMQIQHPTA MIAR+ATAQDD+TGDGTTS VL  GELLRQAERY +EGLHPRV+T+G+++A++ TLK L+ F+
Sbjct:   19 GPRGTLKMLVGGAGQIKLTKDGNVLLHEMQIQHPTATMIARTATAQDDVTGDGTTSTVLLCGELLRQAERYAAEGLHPRVITDGYDVARDATLKFLDEFK 118
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BLAST of mRNA_H-elongata_contig70949.14754.1 vs. uniprot
Match: A0A7S2MSB4_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2MSB4_9STRA)

HSP 1 Score: 173 bits (438), Expect = 3.220e-49
Identity = 84/102 (82.35%), Postives = 94/102 (92.16%), Query Frame = 1 
Query:    1 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDITGDGTTSAVLFTGELLRQAERYLSEGLHPRVLTEGFELAKEHTLKLLEAFRSS 306
            GPRGTLKMLVGGAGQIK+TKDG VLLHEMQIQHPTAM+IAR+ATAQDDITGDGTTSAVL  GELLRQAER  +EGLHPRV+TEGFE+AK+HTL  LE+F+ +
Sbjct:   47 GPRGTLKMLVGGAGQIKITKDGNVLLHEMQIQHPTAMLIARNATAQDDITGDGTTSAVLLCGELLRQAERVYAEGLHPRVITEGFEMAKDHTLDFLESFKKT 148
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BLAST of mRNA_H-elongata_contig70949.14754.1 vs. uniprot
Match: A0A7S3GSF3_9STRA (Hypothetical protein (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3GSF3_9STRA)

HSP 1 Score: 166 bits (420), Expect = 2.320e-48
Identity = 80/100 (80.00%), Postives = 91/100 (91.00%), Query Frame = 1 
Query:    1 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDITGDGTTSAVLFTGELLRQAERYLSEGLHPRVLTEGFELAKEHTLKLLEAFR 300
            GPRGTLKMLVGGAGQIK+TKDG VLLHEMQIQHPTAMMIAR+ATA DD  GDGTTS+VLFTGELL+QAERY SEGLHPR++TEG+E+AK+  L+ LE F+
Sbjct:   52 GPRGTLKMLVGGAGQIKITKDGNVLLHEMQIQHPTAMMIARAATAMDDQVGDGTTSSVLFTGELLKQAERYTSEGLHPRLITEGYEIAKDMVLEFLEGFK 151
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BLAST of mRNA_H-elongata_contig70949.14754.1 vs. uniprot
Match: A0A7S2V196_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V196_9STRA)

HSP 1 Score: 170 bits (430), Expect = 4.370e-48
Identity = 83/100 (83.00%), Postives = 93/100 (93.00%), Query Frame = 1 
Query:    1 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDITGDGTTSAVLFTGELLRQAERYLSEGLHPRVLTEGFELAKEHTLKLLEAFR 300
            GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTA++IAR+ATAQDDITGDGTTS+VL TGELL+QAERYL+EGLHPR LTEGF+LA++  L  LE F+
Sbjct:   40 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAVLIARTATAQDDITGDGTTSSVLLTGELLKQAERYLAEGLHPRSLTEGFDLARDRVLDFLEKFK 139
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BLAST of mRNA_H-elongata_contig70949.14754.1 vs. uniprot
Match: A0A485LAF2_9STRA (Aste57867_17113 protein n=17 Tax=Saprolegniaceae TaxID=4764 RepID=A0A485LAF2_9STRA)

HSP 1 Score: 167 bits (424), Expect = 3.210e-47
Identity = 81/100 (81.00%), Postives = 92/100 (92.00%), Query Frame = 1 
Query:    1 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDITGDGTTSAVLFTGELLRQAERYLSEGLHPRVLTEGFELAKEHTLKLLEAFR 300
            GPRGTLKMLVGGAGQIKLTKDG VLLHEMQIQHPTA +IAR+ATAQDDITGDGTTS VLFTGELL+QAER+LS+GLHPR+L EGFELA+E  L++L+  +
Sbjct:   39 GPRGTLKMLVGGAGQIKLTKDGNVLLHEMQIQHPTAAVIARAATAQDDITGDGTTSTVLFTGELLKQAERFLSDGLHPRILAEGFELAREEALRVLDRIK 138
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BLAST of mRNA_H-elongata_contig70949.14754.1 vs. uniprot
Match: K3WHV4_GLOUD (Uncharacterized protein n=4 Tax=Oomycota TaxID=4762 RepID=K3WHV4_GLOUD)

HSP 1 Score: 167 bits (423), Expect = 3.510e-47
Identity = 80/100 (80.00%), Postives = 92/100 (92.00%), Query Frame = 1 
Query:    1 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDITGDGTTSAVLFTGELLRQAERYLSEGLHPRVLTEGFELAKEHTLKLLEAFR 300
            GPRGTLKMLVGGAGQIKLTKDG VLLHEMQIQHPTA +IAR+ATAQDDITGDGTTS VLF GELL+QAER+L++GLHPR+L EGF+LAK+ TL++LE  +
Sbjct:   38 GPRGTLKMLVGGAGQIKLTKDGNVLLHEMQIQHPTAALIARAATAQDDITGDGTTSTVLFIGELLKQAERFLADGLHPRILAEGFDLAKDETLRILETLK 137
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BLAST of mRNA_H-elongata_contig70949.14754.1 vs. uniprot
Match: A0A6V1PFD7_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1PFD7_HETAK)

HSP 1 Score: 167 bits (423), Expect = 4.360e-47
Identity = 79/100 (79.00%), Postives = 92/100 (92.00%), Query Frame = 1 
Query:    1 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDITGDGTTSAVLFTGELLRQAERYLSEGLHPRVLTEGFELAKEHTLKLLEAFR 300
            GPRGTLKMLVGGAGQIKLTKDG+VLLHEMQIQHPTAM+I R+ATAQDDITGDGTTS ++F GELL+QAER+LSEGLHPRV+TEGF+LA++  L  L+ F+
Sbjct:   39 GPRGTLKMLVGGAGQIKLTKDGKVLLHEMQIQHPTAMLIGRTATAQDDITGDGTTSVIIFCGELLKQAERHLSEGLHPRVITEGFDLARDRVLTFLDGFK 138
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BLAST of mRNA_H-elongata_contig70949.14754.1 vs. uniprot
Match: A0A0L0SC99_ALLM3 (Uncharacterized protein n=1 Tax=Allomyces macrogynus (strain ATCC 38327) TaxID=578462 RepID=A0A0L0SC99_ALLM3)

HSP 1 Score: 162 bits (410), Expect = 5.440e-47
Identity = 79/105 (75.24%), Postives = 96/105 (91.43%), Query Frame = 1 
Query:    1 GPRGTLKMLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDITGDGTTSAVLFTGELLRQAERYLSEGLHPRVLTEGFELAKEHTLKLLEAFRSSRRV 315
            GPRGT+KMLV G+GQIKLTKDG+VLL EMQIQ+PTA MIAR+ATAQD+ITGDGTTS VL  GELL+QAERY+SEGLHPRVLTEGFELAK+ +L+ LE+F+ ++++
Sbjct:   48 GPRGTIKMLVDGSGQIKLTKDGKVLLSEMQIQNPTATMIARAATAQDEITGDGTTSIVLLVGELLKQAERYISEGLHPRVLTEGFELAKKESLQFLESFKLTKKI 152
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig70949.14754.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LIP0_ECTSI5.440e-5691.26Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A418EL66_9STRA2.570e-5083.51Uncharacterized protein n=1 Tax=Aphanomyces astaci... [more]
A0A7R9YWJ8_9STRA3.410e-5079.00Hypothetical protein (Fragment) n=1 Tax=Pseudictyo... [more]
A0A7S2MSB4_9STRA3.220e-4982.35Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A7S3GSF3_9STRA2.320e-4880.00Hypothetical protein (Fragment) n=1 Tax=Spumella e... [more]
A0A7S2V196_9STRA4.370e-4883.00Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A485LAF2_9STRA3.210e-4781.00Aste57867_17113 protein n=17 Tax=Saprolegniaceae T... [more]
K3WHV4_GLOUD3.510e-4780.00Uncharacterized protein n=4 Tax=Oomycota TaxID=476... [more]
A0A6V1PFD7_HETAK4.360e-4779.00Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A0L0SC99_ALLM35.440e-4775.24Uncharacterized protein n=1 Tax=Allomyces macrogyn... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig70949contigH-elongata_contig70949:688..2843 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score178.7
Seed ortholog evalue1.4e-42
Seed eggNOG ortholog2880.D8LIP0
Preferred nameCCT6
KEGG koko:K09498,ko:K19401
GOsGO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0005832,GO:0005856,GO:0006457,GO:0006458,GO:0008150,GO:0009987,GO:0010035,GO:0010038,GO:0010043,GO:0016020,GO:0032991,GO:0042221,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0044183,GO:0044424,GO:0044444,GO:0044445,GO:0044464,GO:0046686,GO:0050896,GO:0051082,GO:0061077,GO:0101031
EggNOG free text desc.unfolded protein binding
EggNOG OGsCOG0459@1,KOG0359@2759
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03036,ko03110,ko04147
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionGroEL-like equatorial domain
Ec32 orthologEc-11_004600.1
Exons2
Model size336
Cds size315
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig70949.14754.1prot_H-elongata_contig70949.14754.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig70949 688..2822 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931108.3559017-CDS-H-elongata_contig70949:687..8851622931108.3559017-CDS-H-elongata_contig70949:687..885Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig70949 688..885 -
1691679701.5268812-CDS-H-elongata_contig70949:687..8851691679701.5268812-CDS-H-elongata_contig70949:687..885Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig70949 688..885 -
1622931108.3709948-CDS-H-elongata_contig70949:2705..28221622931108.3709948-CDS-H-elongata_contig70949:2705..2822Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig70949 2706..2822 -
1691679701.5375218-CDS-H-elongata_contig70949:2705..28221691679701.5375218-CDS-H-elongata_contig70949:2705..2822Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig70949 2706..2822 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931108.3849707-UTR-H-elongata_contig70949:2822..28431622931108.3849707-UTR-H-elongata_contig70949:2822..2843Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig70949 2823..2843 -
1691679701.5468469-UTR-H-elongata_contig70949:2822..28431691679701.5468469-UTR-H-elongata_contig70949:2822..2843Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig70949 2823..2843 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig70949.14754.1

>prot_H-elongata_contig70949.14754.1 ID=prot_H-elongata_contig70949.14754.1|Name=mRNA_H-elongata_contig70949.14754.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=105bp
MLVGGAGQIKLTKDGRVLLHEMQIQHPTAMMIARSATAQDDITGDGTTSA
VLFTGELLRQAERYLSEGLHPRVLTEGFELAKEHTLKLLEAFRSSRRVHA
ARVD*
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mRNA from alignment at H-elongata_contig70949:688..2843-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig70949.14754.1 ID=mRNA_H-elongata_contig70949.14754.1|Name=mRNA_H-elongata_contig70949.14754.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2156bp|location=Sequence derived from alignment at H-elongata_contig70949:688..2843- (Himanthalia elongata Himel1 dioecious)
GGCCCGCGAGGAACACTGAAGATGCTGGTGGGTGGGGCGGGGCAGATCAA
GCTGACCAAGGATGGCCGTGTCCTGCTGCATGAGATGCAGATACAGCACC
CGACGGCGATGATGATCGCACGCAGCGCCACCGCTCAGGTACGCTCGCGT
GGTATAATATGACATAATATGACAGAACAATTCAGTACATCTGGTGTAGG
TAGTAAATGTATGCTAGGTGTAGTAATACCATGCAATGTACAGTGTGCTT
TGTGGTTTATTCTTGTCACTATATACTATTTAGACCTATATACCGTAAAT
TACGGAGATAACTCTCTCCTCCACGCTAATATTCTCCGAGATGTCGAATT
GAACTTATCCTCACCATAATCAAAGCTTTGTTCTTGAAGAATGATTTTGG
GGGTTTAGTAATAGTGGCTGTGTCTTTGTGTCTGTGTGTGTGTGTGTGTG
TGTGTGTGTATGTTTTTTTTTTAAGGTATAAACTCCAACCCTTCGACCAT
CCATGCCTTCTTAGCACCGTAGGGGTGCAGTGCAGTGCAATCCAGAGGAC
CAACTATGGTGGTGGTGGTTGGGGTGATCATCACATTTAGCACTATACAG
TACAGTATATAGCAGTACAGTATAGAGTAAGATCAACCGGATTTGGGTGC
CTTCTGCTCGTGATCAGCTGAACAGGGAAAATACGTTTTCCCTGTCCCCG
GTCGCGCCTGAGAATTTGGTCTCGCGAGACGGGTTCGGCAGTCACGTTCC
GCGTCAGCCTGCTTATTATCTCAACCCAGACTGAATCTCGTGCGTACTCC
TGAGATCCTCTCAATCCTCCCGTTTTCCGCGACGGCGTCCATCTCTTACC
GTCAACCGCCATCGGGTCAATCCCGATTTGATCATGCCACACAATTGCGT
ACCGATGTCGTTTACTACCGAGAGTCCGCCGGCACTCGAGCCAATAGTCC
TCAAGATAGCTAGAGTAACGGGTGCAACCTATTCAGGTAACCCCATGGAA
CAATTATTGTATGCCTCTATCAAGAGGTTCGAATGTGTACCGTACATTAC
ATTACCTCCGTTGAGCATAAAATCGTCACAATGCATGTGATGTGTGATTA
TTCGTGTTCCCCAAAAATAATTAATTGATCCTGTCGACGGTTTCATGTAG
GGGATCCCGTCATTATCCCGTACTCTTTCCATTATTATTGGTGATGATAT
GGGGACTATGACGCAAAACCAAAGCCTAGAGAGTATTTGGGCTTAAAACA
AAATGTTTGTCATTTTTTTGTTCCCCCTCCAATGCCCGCGTTGAATAGTA
ATATAATATTTAGTAATAGTAATAGTAATAGTTTGAATGAGTTATTAATA
GGTAATAAAAATAAATAATTTAGATACTCGTACAATACTGTGTTTTGTTG
GGGGAGCATTGTTTCTCTAGCTAACGCTACCTGATATTAGTATATAATAA
TATAGAAGAGTGGTCACACGTGCACCGCGTGGTGTCGTTGCCTTTGCCGG
CGAAACAATACAGCTATATATATATATATATATATATATATATGTATATA
TGCTGTATAGTGTATGCTGTATAGTGTACCTGGTACAATTTATAATTAAA
TCTACGCATCTGATAAAAGGGGCGTGAGGTGGAGGCTAGGAGATCTGGGG
AAAGTGGGTTGGGGAGAGATTTGGGGAGACGTTTTTTCTTTCCCATCGTA
TCCCCATCCAGAATCACGCCTCTTTAAATTTTTCCGCACGTACCGTGCTA
TACCTCCTTCTCCCACTTTCTCTCTCACACACACACACACACACACTGAT
GCGCTCCCATGCTTCTACTCTTCAATGGTGTTCTACAGTCGTCGTCGATC
GATGCAGCACCATGCAGAAATGTAAAAAAGCTTGCCTTTCGCGTGCGTTT
GCTTTTTTCTTCCTTCTTGGCGTTCGGTTGATGGGGCGTGGGGATGATCC
GGGCGCAGGATGACATCACGGGAGACGGAACAACAAGCGCGGTGCTGTTC
ACGGGCGAGCTGCTTCGGCAGGCGGAGCGCTACCTCAGCGAGGGGCTCCA
CCCTCGAGTACTGACGGAGGGCTTCGAGCTTGCCAAGGAGCACACCCTGA
AACTCCTTGAGGCGTTCCGATCGAGCAGGCGAGTCCACGCAGCACGCGTT
GATTAG
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Coding sequence (CDS) from alignment at H-elongata_contig70949:688..2843-

>mRNA_H-elongata_contig70949.14754.1 ID=mRNA_H-elongata_contig70949.14754.1|Name=mRNA_H-elongata_contig70949.14754.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=630bp|location=Sequence derived from alignment at H-elongata_contig70949:688..2843- (Himanthalia elongata Himel1 dioecious)
ATGCTGGTGGGTGGGGCGGGGCAGATCAAGCTGACCAAGGATGGCCGTGT
CCTGCTGCATGAGATGCAGATACAGCACCCGACGGCGATGATGATCGCAC
GCAGCGCCACCGCTCAGATGCTGGTGGGTGGGGCGGGGCAGATCAAGCTG
ACCAAGGATGGCCGTGTCCTGCTGCATGAGATGCAGATACAGCACCCGAC
GGCGATGATGATCGCACGCAGCGCCACCGCTCAGGATGACATCACGGGAG
ACGGAACAACAAGCGCGGTGCTGTTCACGGGCGAGCTGCTTCGGCAGGCG
GAGCGCTACCTCAGCGAGGGGCTCCACCCTCGAGTACTGACGGAGGGCTT
CGAGCTTGCCAAGGAGCACACCCTGAAACTCCTTGAGGCGTTCCGATCGA
GCAGGCGAGTCCACGCAGCACGCGTTGATTAGGATGACATCACGGGAGAC
GGAACAACAAGCGCGGTGCTGTTCACGGGCGAGCTGCTTCGGCAGGCGGA
GCGCTACCTCAGCGAGGGGCTCCACCCTCGAGTACTGACGGAGGGCTTCG
AGCTTGCCAAGGAGCACACCCTGAAACTCCTTGAGGCGTTCCGATCGAGC
AGGCGAGTCCACGCAGCACGCGTTGATTAG
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