mRNA_H-elongata_contig70358.14692.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig70358.14692.1
Unique NamemRNA_H-elongata_contig70358.14692.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig70358.14692.1 vs. uniprot
Match: A0A6H5JJG7_9PHAE (HSP40 like protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JJG7_9PHAE)

HSP 1 Score: 71.6 bits (174), Expect = 8.670e-12
Identity = 38/73 (52.05%), Postives = 43/73 (58.90%), Query Frame = 1 
Query:   10 QAYEVLGDPEKRNLYDAYGHQGVEAGAAPCSPPRGAAAQRRGWPGGGDPFRGAFRDPFEMFETFFGPAAGRGG 228
            +AYEVLGDPEK+  YD YGH+GV A A       GA A    W    +PF    RDPFEMFE+FFG A    G
Sbjct:   54 EAYEVLGDPEKKRFYDTYGHEGV-ASAEQQQSAEGAGASGAQWYNQQNPFPHGMRDPFEMFESFFGRAGANNG 125
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BLAST of mRNA_H-elongata_contig70358.14692.1 vs. uniprot
Match: D8LSR9_ECTSI (Heat shock protein 40 like protein/ DnaJ domain containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSR9_ECTSI)

HSP 1 Score: 67.4 bits (163), Expect = 7.840e-10
Identity = 36/68 (52.94%), Postives = 41/68 (60.29%), Query Frame = 1 
Query:   10 QAYEVLGDPEKRNLYDAYGHQGVEAGAAPCSPPRGAAAQRRGWPGGGDPFRGAFRDPFEMFETFFGPA 213
            +AYEVLGDPEK+  YD YGH+GV A A       GA A    W    + F    RDPFEMFE+FFG A
Sbjct:   54 EAYEVLGDPEKKRFYDTYGHEGV-ASAEQQQSAEGAGASGAQWYNQHNAFPHGMRDPFEMFESFFGRA 120
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BLAST of mRNA_H-elongata_contig70358.14692.1 vs. uniprot
Match: A0A8J2X0S4_9STRA (Hypothetical protein n=7 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2X0S4_9STRA)

HSP 1 Score: 63.2 bits (152), Expect = 6.630e-8
Identity = 51/134 (38.06%), Postives = 62/134 (46.27%), Query Frame = 1 
Query:    7 VQAYEVLGDPEKRNLYDAYGHQGVEAGAAPCSPPRGAAAQRRGW---------PGGGDPFRGAFR-DPFEMFETFFGP------AAGRGGLFGHASSMRDLFREPAFGRPEEM-------------NHEDMYRG 321
             +AY+VL DP+KR +YD YG +G++ G+ P       AA RR           PGGG   R  FR DP EMF  FFG       +AG GGL G    M DLF      R E               + ED+YRG
Sbjct:   60 AEAYDVLSDPQKRQIYDVYGVEGLKGGSGPADEAYAEAASRRAGSTAGFAGTAPGGG--MRYEFRGDPSEMFAQFFGAGFARQRSAGEGGLGG----MGDLFGGAGGARREGQRLQRPVVQVALGCSLEDLYRG 187
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BLAST of mRNA_H-elongata_contig70358.14692.1 vs. uniprot
Match: A0A4S2M545_OPIFE (J domain-containing protein (Fragment) n=2 Tax=Opisthorchiidae TaxID=6196 RepID=A0A4S2M545_OPIFE)

HSP 1 Score: 59.7 bits (143), Expect = 7.850e-8
Identity = 39/106 (36.79%), Postives = 52/106 (49.06%), Query Frame = 1 
Query:    7 VQAYEVLGDPEKRNLYDAYGHQGVEAGAAPCSPPRGAAAQRRGWPGGGDPFRGAFR-DPFEMFETFFGPAAGRGGLFGHASSMRDLFREPAFGRPEEMNHEDMYRG 321
             +AY+VL DP+KR +YD YG +G++ G +             G  GGG  F   F  DP EMF  FFG     G LFG  S  R +F     G  E+M+ +  + G
Sbjct:   49 AEAYDVLSDPKKREIYDKYGEEGLKTGVS-------------GGEGGGTGFTYTFHGDPREMFRVFFGSDDTLGSLFGMGSGGRTVFTS---GMGEQMDIDGDFFG 138
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BLAST of mRNA_H-elongata_contig70358.14692.1 vs. uniprot
Match: A0A1F5ZX08_9BACT (J domain-containing protein n=1 Tax=Candidatus Gottesmanbacteria bacterium RIFCSPHIGHO2_02_FULL_39_11 TaxID=1798382 RepID=A0A1F5ZX08_9BACT)

HSP 1 Score: 60.8 bits (146), Expect = 1.930e-7
Identity = 36/85 (42.35%), Postives = 45/85 (52.94%), Query Frame = 1 
Query:   10 QAYEVLGDPEKRNLYDAYGHQGVEAGAAPCSPPRGAAAQRRGWP----------GGGDPFRGA----FRDPFEMFETFFGPAAGR 222
            +AYEVLGDP+K+++YD YG    + G A    P G     +  P          GGG PF G     F DPFE+FE+FFG   GR
Sbjct:   49 KAYEVLGDPKKKDMYDQYGEAAFKPGVAGGGYPGGGQQGGQWGPFSYTYTTNGGGGGSPFEGVDFGGFSDPFEIFESFFGGGFGR 133
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BLAST of mRNA_H-elongata_contig70358.14692.1 vs. uniprot
Match: A0A074ZC20_9TREM (DnaJ domain protein n=2 Tax=Opisthorchiidae TaxID=6196 RepID=A0A074ZC20_9TREM)

HSP 1 Score: 60.5 bits (145), Expect = 3.190e-7
Identity = 39/106 (36.79%), Postives = 52/106 (49.06%), Query Frame = 1 
Query:    7 VQAYEVLGDPEKRNLYDAYGHQGVEAGAAPCSPPRGAAAQRRGWPGGGDPFRGAFR-DPFEMFETFFGPAAGRGGLFGHASSMRDLFREPAFGRPEEMNHEDMYRG 321
             +AY+VL DP+KR +YD YG +G++ G +             G  GGG  F   F  DP EMF  FFG     G LFG  S  R +F     G  E+M+ +  + G
Sbjct:   49 AEAYDVLSDPKKREIYDKYGEEGLKTGVS-------------GGEGGGPGFTYTFHGDPREMFRVFFGSDDSLGSLFGMGSGGRTVFTS---GMGEQMDIDGDFFG 138
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BLAST of mRNA_H-elongata_contig70358.14692.1 vs. uniprot
Match: A0A1G1WGN2_9BACT (Chaperone protein DnaJ n=1 Tax=Candidatus Woykebacteria bacterium RBG_16_44_10 TaxID=1802597 RepID=A0A1G1WGN2_9BACT)

HSP 1 Score: 60.1 bits (144), Expect = 4.570e-7
Identity = 35/74 (47.30%), Postives = 43/74 (58.11%), Query Frame = 1 
Query:   10 QAYEVLGDPEKRNLYDAYGHQGVEAGAAPCSPPRGAAAQRRGWPGGGDPFR-GAFRDPFEMFETFFGPAAGRGG 228
            +AY+VL DP+KR  YD +GH   EAGAA    P G   +    PG    F  G FRDPF++FE FFG  +  GG
Sbjct:   52 EAYQVLSDPQKREAYDRFGHAAFEAGAAGAGAPGGF--RYEAGPGVEFDFDFGGFRDPFDIFEEFFGARSPFGG 123
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BLAST of mRNA_H-elongata_contig70358.14692.1 vs. uniprot
Match: A0A5A7RFI9_STRAF (Potyviral capsid protein interacting protein 1 n=1 Tax=Striga asiatica TaxID=4170 RepID=A0A5A7RFI9_STRAF)

HSP 1 Score: 59.7 bits (143), Expect = 5.360e-7
Identity = 37/106 (34.91%), Postives = 58/106 (54.72%), Query Frame = 1 
Query:   10 QAYEVLGDPEKRNLYDAYGHQGVEAGAAPCSPPRGAAAQRRGWPGGGDPFRGAFRDPFEMFETFFGPAAGRGGLFGHASSMRD--LFREPAFGRPEEMNHEDMYRG 321
            +AY+VLG P+KR +YD YG +G+++G     PP G+  +  G  GGG  FR + RD  ++FE FFG   G  G+   A++     L +          + E++Y+G
Sbjct:   52 EAYDVLGHPDKRQIYDLYGEEGLKSG---MYPPPGSKDRDFGGGGGGRGFRFSPRDAEDIFEEFFGGLDGEKGMSSAAAAAGGGRLMKAEVMETKLPCSLEELYKG 154
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BLAST of mRNA_H-elongata_contig70358.14692.1 vs. uniprot
Match: A0A5J9TUL5_9POAL (Uncharacterized protein (Fragment) n=7 Tax=PACMAD clade TaxID=147370 RepID=A0A5J9TUL5_9POAL)

HSP 1 Score: 60.1 bits (144), Expect = 5.470e-7
Identity = 41/105 (39.05%), Postives = 54/105 (51.43%), Query Frame = 1 
Query:   10 QAYEVLGDPEKRNLYDAYGHQGVEAGAAPCSPPRGAAAQRRGWPGGGDPFRGAFRDPFEMFETFFGPAAGRGGLFGHASS-MRDLFREPAFGRPEEMNHEDMYRG 321
            QAYEVL DPEKR++YD YG  G++ G                  GGGD     F +PF++FE FFG     GG FG +SS +R   R        +++ ED+Y G
Sbjct:  155 QAYEVLSDPEKRDIYDEYGEDGLKEGMG----------------GGGD-----FHNPFDIFEQFFG-----GGAFGGSSSRVRRQKRGEDVVHTLKVSLEDVYNG 233
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BLAST of mRNA_H-elongata_contig70358.14692.1 vs. uniprot
Match: X1K1Y8_9ZZZZ (J domain-containing protein (Fragment) n=1 Tax=marine sediment metagenome TaxID=412755 RepID=X1K1Y8_9ZZZZ)

HSP 1 Score: 57.0 bits (136), Expect = 6.830e-7
Identity = 33/87 (37.93%), Postives = 43/87 (49.43%), Query Frame = 1 
Query:   10 QAYEVLGDPEKRNLYDAYGHQGVEAGAAPCSPPRGAAAQRRGWPGGGDPFRGAFRDPFEMFETFFGPAAGRGGLFGHASSMRDLFRE 270
            +AY++L DPEKR +YD YGH G+E+                   G G  FRG   DP ++FE  FG  A  G +FG+    R   RE
Sbjct:   53 EAYKILSDPEKRQIYDQYGHAGLESEV-----------------GAGAGFRGFDFDPLKIFEEVFGRGAFGGDIFGNFFGRRATVRE 122
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig70358.14692.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JJG7_9PHAE8.670e-1252.05HSP40 like protein n=1 Tax=Ectocarpus sp. CCAP 131... [more]
D8LSR9_ECTSI7.840e-1052.94Heat shock protein 40 like protein/ DnaJ domain co... [more]
A0A8J2X0S4_9STRA6.630e-838.06Hypothetical protein n=7 Tax=Pelagomonas calceolat... [more]
A0A4S2M545_OPIFE7.850e-836.79J domain-containing protein (Fragment) n=2 Tax=Opi... [more]
A0A1F5ZX08_9BACT1.930e-742.35J domain-containing protein n=1 Tax=Candidatus Got... [more]
A0A074ZC20_9TREM3.190e-736.79DnaJ domain protein n=2 Tax=Opisthorchiidae TaxID=... [more]
A0A1G1WGN2_9BACT4.570e-747.30Chaperone protein DnaJ n=1 Tax=Candidatus Woykebac... [more]
A0A5A7RFI9_STRAF5.360e-734.91Potyviral capsid protein interacting protein 1 n=1... [more]
A0A5J9TUL5_9POAL5.470e-739.05Uncharacterized protein (Fragment) n=7 Tax=PACMAD ... [more]
X1K1Y8_9ZZZZ6.830e-737.93J domain-containing protein (Fragment) n=1 Tax=mar... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig70358contigH-elongata_contig70358:366..2680 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score68.6
Seed ortholog evalue4.3e-09
Seed eggNOG ortholog2880.D8LSR9
KEGG koko:K09512
EggNOG free text desc.protein folding
EggNOG OGsCOG0484@1,KOG0714@2759
Ec32 ortholog descriptionHeat shock protein 40 like protein/ DnaJ domain containing protein
Ec32 orthologEc-15_002850.1
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03110
Hectar predicted targeting categoryother localisation
Exons2
Model size663
Cds size663
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931101.6145167-CDS-H-elongata_contig70358:365..7481622931101.6145167-CDS-H-elongata_contig70358:365..748Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig70358 366..748 +
1691679698.5807006-CDS-H-elongata_contig70358:365..7481691679698.5807006-CDS-H-elongata_contig70358:365..748Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig70358 366..748 +
1622931101.6323986-CDS-H-elongata_contig70358:2400..26801622931101.6323986-CDS-H-elongata_contig70358:2400..2680Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig70358 2401..2680 +
1691679698.5955598-CDS-H-elongata_contig70358:2400..26801691679698.5955598-CDS-H-elongata_contig70358:2400..2680Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig70358 2401..2680 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig70358.14692.1prot_H-elongata_contig70358.14692.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig70358 366..2680 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig70358.14692.1

>prot_H-elongata_contig70358.14692.1 ID=prot_H-elongata_contig70358.14692.1|Name=mRNA_H-elongata_contig70358.14692.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=221bp
MPVQAYEVLGDPEKRNLYDAYGHQGVEAGAAPCSPPRGAAAQRRGWPGGG
DPFRGAFRDPFEMFETFFGPAAGRGGLFGHASSMRDLFREPAFGRPEEMN
HEDMYRGRGGGGAGRNEGMFGGKRGGGFCRPFSCPNPAGDPFFRGGSMHQ
SMFGGFDDVFGRGFGGASGFGGASGFGGAGGGFSSFSSSSTTVGPDGVPR
TVQRTEKTVINADGTRETKVR
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mRNA from alignment at H-elongata_contig70358:366..2680+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig70358.14692.1 ID=mRNA_H-elongata_contig70358.14692.1|Name=mRNA_H-elongata_contig70358.14692.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2315bp|location=Sequence derived from alignment at H-elongata_contig70358:366..2680+ (Himanthalia elongata Himel1 dioecious)
ATGCCCGTGCAGGCCTACGAAGTCCTTGGAGACCCCGAGAAGAGAAACTT
GTACGACGCCTACGGCCACCAGGGCGTGGAGGCGGGGGCGGCCCCTTGTA
GCCCTCCGAGAGGCGCAGCGGCACAGCGAAGAGGGTGGCCTGGGGGGGGA
GATCCGTTCCGCGGAGCCTTCCGCGACCCGTTCGAGATGTTCGAGACCTT
CTTCGGACCTGCCGCCGGGAGAGGAGGGCTGTTTGGGCACGCCTCGAGCA
TGCGCGATTTATTCCGGGAGCCGGCTTTCGGCCGTCCCGAAGAGATGAAC
CACGAAGACATGTATCGGGGACGCGGTGGCGGTGGTGCCGGCAGAAACGA
GGGCATGTTCGGCGGTAAGAGGGGGGGGGGGTTGTCTCTTGATTACAATG
GGTACACTTGGGTTTCCCATCCCCCCTAAGGTACTGCACCGTTTCGTAAC
GGATCAACCCATCACCTTTCCCCGGTTGGGTAACGCCTTCAATTTTTGGG
GAATATTCTGATTGTGACGTTTTTTGTTTGTTGTGTCGTGGGTGTTGCAC
TATCGAGGCATATTCTGGGTGTTGCGCGATCGCGGGATAATCATCAATAA
CACACAAAACGAGAAGCGTTCAATAATTGGTCCATGGGGCTACCTGAATA
GGCTGCACCCGTTACTCGAGCTACCTTGAGGACTGCTGGCTCAGTGCCGG
CGGACTCTCGGTAGGTAGTAAACGCCATCGGTACGCAATTGTGTGACCTG
ATGAAATCGGGACTGACCCGATGGCGGTTGACGGTAGAGATGGACGCCGT
CGCGGAAAACGAGAGGATCAAAAGGATCCCGGGAGTAGGCTGCACCCCTT
ACACAAGCTATCTTGAGGTCTGCTGGCTCAGTGCTGGCGGACTCTCGGTG
GTGAACGACATTGGAACGCAATTGCGTGATCTGATGAAATCGGGATTGAC
CCGATTGGAGTGGACGGTAGAAATGGATGCTGTCGCGGAAAACGAGAGGA
TCGAGAGGATCCCGGGAGTACGCAAAAGAATTAGCCTGGGTTGGGAAAAT
TAGCGGGCTGACGAGGGACGGGACTGCCGAACTCTTTTTGCGAGACCAAA
TTCTCAGGCACAAACGGGGACAGGAAAAACTCACTTTTTTCTTGTTCAGC
TGACCACGAGCAAGAGGCAACCAAACCCGGTTGGTCCATACTTTGCTATA
TGTGATGGCCTCATGACCACACACCAAGCGTGGCGACATTCTTCTGGGCC
ATGCAGTGCGGTGTAATCCCCATAAGGCCGGGTCTTATCAGAGCACAAAA
AAAATAATTTCCAGCCGATTTAAATTTAAATCGGGCTGTGATTGGTCGAA
AGCAAATAACGCCTAGCCTTTGTCCAATCAGAGCGCGATTTAAATTTAAA
TCGGCTGTAAATTTAAATTTATGTATTCCGATAAGACCCGGCCTAATGAC
CACCACCCCTCTCGTAATCATGCCGTCAGAAATGCGACGTCACATCAAGT
GCACCCATGAAGTTTGACGGGGGGGAGGGGGAGGATTGTAATGGTGTAAT
CATGATTGCTGGGGCTAGTGACGTGCAATAAATGCGTGGCCACGCAAACC
TGATGATGGTTGGTCCCCCAGATAATAGTTGTTCCCTTGGATTTCGCTAA
AACCGGTAAACTTATGGTGTGGATGTTTGAAGGGTTCAAATGAAGTAATA
TATTTTTTGAAAGAAGAATCGTGGCGTCATGGGATTATGATCGTTACGAG
TAAAATGGTGATCGTGACGTCGTTGCGTGATTGGTCATGAGTGGTATCGC
TTTTGAACCATGACGTCATCTCGTTCGATCGTGCGGTAGAAGGGGACACC
ATCAACACAAGGAACTGTAATACAAGCCAGGCAAAAAGCGACAAATCAAA
CACAAACGGTCACTTACGTGTGGCGTCATCCGTGGTAGGTCACACTACTA
ATAGAGCGAGTCACACCGCGAGCGTGTGGCGTTGCTGCTGTGTACAGTAT
CGTGGACTGTATGTATGAAAAGTCATCGTTCACAGTTGCCGTCCCTTTTC
GTGTCCCAACCCGGCAGGCGATCCCTTCTTCCGAGGCGGTTCCATGCACC
AGAGCATGTTCGGAGGATTTGACGACGTTTTCGGTCGCGGTTTCGGAGGA
GCAAGCGGTTTTGGAGGAGCGAGCGGTTTTGGAGGAGCTGGCGGGGGGTT
CTCGTCCTTCAGCTCCAGCTCGACGACCGTGGGACCGGACGGTGTGCCGC
GGACTGTTCAGCGAACCGAGAAGACTGTCATCAACGCGGACGGGACGCGC
GAGACTAAGGTGCGT
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Coding sequence (CDS) from alignment at H-elongata_contig70358:366..2680+

>mRNA_H-elongata_contig70358.14692.1 ID=mRNA_H-elongata_contig70358.14692.1|Name=mRNA_H-elongata_contig70358.14692.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=1326bp|location=Sequence derived from alignment at H-elongata_contig70358:366..2680+ (Himanthalia elongata Himel1 dioecious)
ATGCCCGTGCAGGCCTACGAAGTCCTTGGAGACCCCGAGAAGAGAAACTT
GTACGACGCCTACGGCCACCAGGGCGTGGAGGCGGGGGCGGCCCCTTGTA
GCCCTCCGAGAGGCGCAGCGGCACAGCGAAGAGGGTGGCCTGGGGGGGGA
GATCCGTTCCGCGGAGCCTTCCGCGACCCGTTCGAGATGTTCGAGACCTT
CTTCGGACCTGCCGCCGGGAGAGGAGGGCTGTTTGGGCACGCCTCGAGCA
TGCGCGATTTATTCCGGGAGCCGGCTTTCGGCCGTCCCGAAGAGATGAAC
CACGAAGACATGTATCGGGGACGCGGTGGCGGTGGTGCCGGCAGAAACGA
GGGCATGTTCGGCGGTAAGAGGGGGGGGGGGTTATGCCCGTGCAGGCCTA
CGAAGTCCTTGGAGACCCCGAGAAGAGAAACTTGTACGACGCCTACGGCC
ACCAGGGCGTGGAGGCGGGGGCGGCCCCTTGTAGCCCTCCGAGAGGCGCA
GCGGCACAGCGAAGAGGGTGGCCTGGGGGGGGAGATCCGTTCCGCGGAGC
CTTCCGCGACCCGTTCGAGATGTTCGAGACCTTCTTCGGACCTGCCGCCG
GGAGAGGAGGGCTGTTTGGGCACGCCTCGAGCATGCGCGATTTATTCCGG
GAGCCGGCTTTCGGCCGTCCCGAAGAGATGAACCACGAAGACATGTATCG
GGGACGCGGTGGCGGTGGTGCCGGCAGAAACGAGGGCATGTTCGGCGGTA
AGAGGGGGGGGGGGTTTTGCCGTCCCTTTTCGTGTCCCAACCCGGCAGGC
GATCCCTTCTTCCGAGGCGGTTCCATGCACCAGAGCATGTTCGGAGGATT
TGACGACGTTTTCGGTCGCGGTTTCGGAGGAGCAAGCGGTTTTGGAGGAG
CGAGCGGTTTTGGAGGAGCTGGCGGGGGGTTCTCGTCCTTCAGCTCCAGC
TCGACGACCGTGGGACCGGACGGTGTGCCGCGGACTGTTCAGCGAACCGA
GAAGACTGTCATCAACGCGGACGGGACGCGCGAGACTAAGGTGCGTTTGC
CGTCCCTTTTCGTGTCCCAACCCGGCAGGCGATCCCTTCTTCCGAGGCGG
TTCCATGCACCAGAGCATGTTCGGAGGATTTGACGACGTTTTCGGTCGCG
GTTTCGGAGGAGCAAGCGGTTTTGGAGGAGCGAGCGGTTTTGGAGGAGCT
GGCGGGGGGTTCTCGTCCTTCAGCTCCAGCTCGACGACCGTGGGACCGGA
CGGTGTGCCGCGGACTGTTCAGCGAACCGAGAAGACTGTCATCAACGCGG
ACGGGACGCGCGAGACTAAGGTGCGT
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