mRNA_H-elongata_contig69948.14640.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig69948.14640.1
Unique NamemRNA_H-elongata_contig69948.14640.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig69948.14640.1 vs. uniprot
Match: A0A6H5KP27_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KP27_9PHAE)

HSP 1 Score: 212 bits (539), Expect = 2.250e-61
Identity = 91/116 (78.45%), Postives = 107/116 (92.24%), Query Frame = 1 
Query:    1 LGVKGGMHAVGHALLSVVPLFLLCDPEDVDCEHVRPHNQRPQPRRILVYDKRPGGVGVADAMFGCHRAALTKALELVEGCPCPEGCVACIHDHGCTGYNSIIDKQAALIVLRRLIE 348
            L ++GG+HAVGHALL+VVPLFLLCD  DVDCEH RPH+ RPQP+RILV+DKRPGGVGV+DAMFGCHR  L+KALELVEGCPCPEGC+ACIHDHGCTGYN+IIDK+AA+++L  LI+
Sbjct: 1253 LDLEGGIHAVGHALLAVVPLFLLCDAADVDCEHARPHHSRPQPQRILVFDKRPGGVGVSDAMFGCHRGVLSKALELVEGCPCPEGCLACIHDHGCTGYNAIIDKRAAVVILESLIQ 1368
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BLAST of mRNA_H-elongata_contig69948.14640.1 vs. uniprot
Match: D8LLG5_ECTSI (DEAD/DEAH box helicase domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LLG5_ECTSI)

HSP 1 Score: 190 bits (483), Expect = 6.220e-54
Identity = 80/100 (80.00%), Postives = 93/100 (93.00%), Query Frame = 1 
Query:    1 LGVKGGMHAVGHALLSVVPLFLLCDPEDVDCEHVRPHNQRPQPRRILVYDKRPGGVGVADAMFGCHRAALTKALELVEGCPCPEGCVACIHDHGCTGYNS 300
            L ++GG+HAVGHALL+VVPLFLLCD  DVDCEH RPH+ RPQP+RILV+D+RPGGVGV+DAMFGCHR  L+KALELVEGCPCP+GC+ACIHDHGCTGYN+
Sbjct:  811 LDLEGGIHAVGHALLAVVPLFLLCDAADVDCEHARPHHSRPQPQRILVFDRRPGGVGVSDAMFGCHRGVLSKALELVEGCPCPDGCLACIHDHGCTGYNA 910
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BLAST of mRNA_H-elongata_contig69948.14640.1 vs. uniprot
Match: A0A7S2AHT8_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2AHT8_9STRA)

HSP 1 Score: 130 bits (328), Expect = 6.980e-34
Identity = 54/110 (49.09%), Postives = 79/110 (71.82%), Query Frame = 1 
Query:    7 VKGGMHAVGHALLSVVPLFLLCDPEDVDCEHVRPHNQRPQPRRILVYDKRPGGVGVADAMFGC-HRAALTKALELVEGCPCPEGCVACIHDHGCTGYNSIIDKQAALIVL 333
            V+  +HA+ H L+++ PLF+LCD +D+D EHV  + QRP+P R++VYDKRPGGVG+A+A++ C   A L+KA +LV  C C  GC  CIHD  C+G N  + K+ A+++L
Sbjct:  241 VRSAVHALNHVLVAIAPLFILCDKDDIDTEHVYVYQQRPRPARLIVYDKRPGGVGIAEALYCCGSHAVLSKAYDLVTSCSCAYGCPGCIHDPRCSGQNESLFKEGAILIL 350
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BLAST of mRNA_H-elongata_contig69948.14640.1 vs. uniprot
Match: A0A4D9D1F7_9STRA (Helicase ATP-binding domain-containing protein (Fragment) n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D1F7_9STRA)

HSP 1 Score: 129 bits (325), Expect = 2.610e-33
Identity = 56/114 (49.12%), Postives = 79/114 (69.30%), Query Frame = 1 
Query:   13 GGMHAVGHALLSVVPLFLLCDPEDVDCEHVRPHNQRPQPRRILVYDKRPGGVGVADAMFGCHRAALTKALELVEGCPCP--EGCVACIHDHGCTGYNSIIDKQAALIVLRRLIE 348
            GG+HA+ HALL+V PLFL CDP D++ EH     QRP+P R+ VYDKRPGG+G +D +F      L++AL L+  CPC    GC +C+    C+ YN+++ K AAL++LRRL++
Sbjct:  285 GGLHAINHALLAVTPLFLKCDPGDIEAEHAHVQYQRPRPPRVCVYDKRPGGIGASDGLFSALPHVLSQALALLASCPCDLLSGCPSCLVLPSCSSYNTVLHKPAALLLLRRLLD 398
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BLAST of mRNA_H-elongata_contig69948.14640.1 vs. uniprot
Match: F0W7W2_9STRA (DEAD/DEAH box RNA helicase putative n=1 Tax=Albugo laibachii Nc14 TaxID=890382 RepID=F0W7W2_9STRA)

HSP 1 Score: 120 bits (300), Expect = 3.580e-29
Identity = 50/113 (44.25%), Postives = 71/113 (62.83%), Query Frame = 1 
Query:   10 KGGMHAVGHALLSVVPLFLLCDPEDVDCEHVRPHNQRPQPRRILVYDKRPGGVGVADAMFGCHRAALTKALELVEGCPCPEGCVACIHDHGCTGYNSIIDKQAALIVLRRLIE 348
             G +H VGH L S+VPLF+ CDP D+D EH  P  QR +PRRI +Y+KR GG G+   +       +TK+  L+  C C EGC +C+H   CT +N ++DK+ A  +L+ +IE
Sbjct:  934 NGSLHGVGHLLTSLVPLFIRCDPGDIDTEHYHPKEQRSRPRRITMYEKREGGTGIVSELQSVFAELVTKSFALLIECSCLEGCPSCVHSTRCTEHNEVLDKEGARCILKFIIE 1046
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BLAST of mRNA_H-elongata_contig69948.14640.1 vs. uniprot
Match: A0A8K1CRR0_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CRR0_PYTOL)

HSP 1 Score: 119 bits (299), Expect = 4.860e-29
Identity = 52/113 (46.02%), Postives = 72/113 (63.72%), Query Frame = 1 
Query:   10 KGGMHAVGHALLSVVPLFLLCDPEDVDCEHVRPHNQRPQPRRILVYDKRPGGVGVADAMFGCHRAALTKALELVEGCPCPEGCVACIHDHGCTGYNSIIDKQAALIVLRRLIE 348
             G +H VGH LL+VVPLF+LCD  D+D EH  P  +R +P RI +Y+ R GG G+ + +     A L KA  + + C C  GC +CIH   CT YN+++DK+ +LIVL  L+E
Sbjct:  890 SGALHGVGHLLLAVVPLFVLCDAGDIDSEHYNPFERRSRPNRITIYEHREGGSGIVEEIVRSLPAILFKAWTIADECSCIVGCPSCIHSAQCTEYNNVLDKRGSLIVLEHLVE 1002
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BLAST of mRNA_H-elongata_contig69948.14640.1 vs. uniprot
Match: A0A0G4EC89_VITBC (Uncharacterized protein n=3 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4EC89_VITBC)

HSP 1 Score: 119 bits (299), Expect = 4.970e-29
Identity = 53/115 (46.09%), Postives = 75/115 (65.22%), Query Frame = 1 
Query:    1 LGVKGGMHAVGHALLSVVPLFLLCDPEDVDCEHVRPHNQRPQPRRILVYDKRPGGVGVADAMFGCHRAALTKALELVEGCPCPEGCVACIHDHGCTGYNSIIDKQAALIVLRRLI 345
            L ++GG+H   HALL+VVPLF+LCD  D+ CE   P ++R +P R++V+D  PGGVG++   F   R     AL+LV  C C +GC AC+    C  YN++I K AAL+VLR ++
Sbjct: 1451 LDIRGGIHGAEHALLAVVPLFVLCDRTDISCECPNPWDERLRPLRLVVFDSTPGGVGISMRAFQQARGIARAALQLVNDCTCDQGCPACVCHPNCGEYNAVISKPAALVVLRAMV 1565
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BLAST of mRNA_H-elongata_contig69948.14640.1 vs. uniprot
Match: A0A5A8EQ68_CAFRO (Uncharacterized protein n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8EQ68_CAFRO)

HSP 1 Score: 119 bits (297), Expect = 7.570e-29
Identity = 52/111 (46.85%), Postives = 72/111 (64.86%), Query Frame = 1 
Query:   13 GGMHAVGHALLSVVPLFLLCDPEDVDCEHVRPHNQRPQPRRILVYDKRPGGVGVADAMFGCHRAALTKALELVEGCPCPEGCVACIHDHGCTGYNSIIDKQAALIVLRRLI 345
            GG HA  HAL++ +P F+LCD  D+  +   P  +R +P R++VYD R GG+GVA A F   R    +AL ++E CPC  GC +C HD  C GYN +IDK AA+I+LR ++
Sbjct:  488 GGCHAASHALVAALPQFVLCDRSDLGMDCANPLAERARPLRVMVYDNRMGGIGVAAAAFQRARRMFRQALAILESCPCVPGCPSCCHDPACRGYNYVIDKPAAIIILRGVL 598
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BLAST of mRNA_H-elongata_contig69948.14640.1 vs. uniprot
Match: A0A7S3NQ71_9STRA (Hypothetical protein n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3NQ71_9STRA)

HSP 1 Score: 117 bits (293), Expect = 3.130e-28
Identity = 51/118 (43.22%), Postives = 80/118 (67.80%), Query Frame = 1 
Query:    1 LGVKGGMHAVGHALLSVVPLFLLCDPEDVDCEHVRPHNQRPQPRRILVYDKRPGGVGVADAMFGCHRAALTKALELVEGCPC--PEGCVACIHDHGCTGYNSIIDKQAALIVLRRLIE 348
            L +  G+HA  HA+L++VPLF++CD  DV+ EHV    +RP+P RI+++D RPGG+GVA A+F    A +++AL L+  CPC    GC +C+    CT +N  +D +AA+I+ R +++
Sbjct:  753 LDLHHGIHACTHAILNIVPLFVVCDNTDVNAEHVYISQKRPRPARIIIFDTRPGGIGVAHAIFRRIYAVISEALRLITECPCHAQSGCPSCLFSASCTAHNLALDSRAAIIIFRAILK 870
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BLAST of mRNA_H-elongata_contig69948.14640.1 vs. uniprot
Match: A0A1Y1IHD6_KLENI (ATP-dependent helicase n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1IHD6_KLENI)

HSP 1 Score: 115 bits (287), Expect = 2.080e-27
Identity = 50/116 (43.10%), Postives = 74/116 (63.79%), Query Frame = 1 
Query:    4 GVKGGMHAVGHALLSVVPLFLLCDPEDVDCEHVRPHNQRPQPRRILVYDKRPGGVGVADAMFGCHRAALTKALELVEGCPCP--EGCVACIHDHGCTGYNSIIDKQAALIVLRRLI 345
            G + G+HA GHA+L+V+PL+++C P D+ CE   P + R +P R+LVYDK+P G+G+A          L  ALEL+E C C   +GC  C+H  GC  YN  +DK+ A+ +L+ +I
Sbjct: 1843 GFREGLHAAGHAILNVLPLYVMCSPNDIGCECASPDDTRFRPERLLVYDKQPDGIGIAVQARPMFAELLQAALELLEACECTLSDGCPGCVHHTGCGEYNRCLDKRYAIAILKSVI 1958
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig69948.14640.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KP27_9PHAE2.250e-6178.45Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LLG5_ECTSI6.220e-5480.00DEAD/DEAH box helicase domain-containing protein n... [more]
A0A7S2AHT8_9STRA6.980e-3449.09Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A4D9D1F7_9STRA2.610e-3349.12Helicase ATP-binding domain-containing protein (Fr... [more]
F0W7W2_9STRA3.580e-2944.25DEAD/DEAH box RNA helicase putative n=1 Tax=Albugo... [more]
A0A8K1CRR0_PYTOL4.860e-2946.02Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A0G4EC89_VITBC4.970e-2946.09Uncharacterized protein n=3 Tax=Vitrella brassicaf... [more]
A0A5A8EQ68_CAFRO7.570e-2946.85Uncharacterized protein n=1 Tax=Cafeteria roenberg... [more]
A0A7S3NQ71_9STRA3.130e-2843.22Hypothetical protein n=1 Tax=Aureoumbra lagunensis... [more]
A0A1Y1IHD6_KLENI2.080e-2743.10ATP-dependent helicase n=1 Tax=Klebsormidium niten... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig69948contigH-elongata_contig69948:447..1518 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score184.5
Seed ortholog evalue2.7e-44
Seed eggNOG ortholog2880.D8LLG5
KEGG koko:K06877
GOsGO:0000166,GO:0000217,GO:0000405,GO:0003674,GO:0003676,GO:0003677,GO:0003678,GO:0003724,GO:0003824,GO:0004003,GO:0004004,GO:0004386,GO:0005488,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005694,GO:0005730,GO:0005737,GO:0006139,GO:0006259,GO:0006281,GO:0006289,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0006996,GO:0008026,GO:0008094,GO:0008144,GO:0008150,GO:0008152,GO:0008186,GO:0009314,GO:0009411,GO:0009416,GO:0009628,GO:0009987,GO:0010501,GO:0016043,GO:0016070,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017076,GO:0017111,GO:0030554,GO:0031974,GO:0031981,GO:0032392,GO:0032508,GO:0032553,GO:0032555,GO:0032559,GO:0033554,GO:0034641,GO:0034644,GO:0035639,GO:0035861,GO:0036094,GO:0036297,GO:0042623,GO:0043138,GO:0043140,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044237,GO:0044238,GO:0044260,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044446,GO:0044464,GO:0046483,GO:0050896,GO:0051276,GO:0051716,GO:0070013,GO:0070035,GO:0070914,GO:0071103,GO:0071214,GO:0071478,GO:0071482,GO:0071704,GO:0071840,GO:0090304,GO:0090734,GO:0097159,GO:0097367,GO:0104004,GO:0140097,GO:0140098,GO:1901255,GO:1901265,GO:1901360,GO:1901363
EggNOG free text desc.nucleotide-excision repair involved in interstrand cross-link repair
EggNOG OGsCOG1205@1,KOG4150@2759
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000
Hectar predicted targeting categorysignal peptide
Ec32 ortholog descriptionHelicase, C-terminal
Ec32 orthologEc-27_002550.1
Exons3
Model size348
Cds size330
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig69948.14640.1prot_H-elongata_contig69948.14640.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig69948 465..1518 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931096.723974-UTR-H-elongata_contig69948:446..4641622931096.723974-UTR-H-elongata_contig69948:446..464Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig69948 447..464 +
1691679696.472347-UTR-H-elongata_contig69948:446..4641691679696.472347-UTR-H-elongata_contig69948:446..464Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig69948 447..464 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931096.742801-CDS-H-elongata_contig69948:464..6461622931096.742801-CDS-H-elongata_contig69948:464..646Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig69948 465..646 +
1691679696.4842072-CDS-H-elongata_contig69948:464..6461691679696.4842072-CDS-H-elongata_contig69948:464..646Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig69948 465..646 +
1622931096.7595317-CDS-H-elongata_contig69948:911..10111622931096.7595317-CDS-H-elongata_contig69948:911..1011Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig69948 912..1011 +
1691679696.495405-CDS-H-elongata_contig69948:911..10111691679696.495405-CDS-H-elongata_contig69948:911..1011Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig69948 912..1011 +
1622931096.7736285-CDS-H-elongata_contig69948:1470..15181622931096.7736285-CDS-H-elongata_contig69948:1470..1518Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig69948 1471..1518 +
1691679696.5069766-CDS-H-elongata_contig69948:1470..15181691679696.5069766-CDS-H-elongata_contig69948:1470..1518Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig69948 1471..1518 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig69948.14640.1

>prot_H-elongata_contig69948.14640.1 ID=prot_H-elongata_contig69948.14640.1|Name=mRNA_H-elongata_contig69948.14640.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=110bp
MHAVGHALLSVVPLFLLCDPEDVDCEHVRPHNQRPQPRRILVYDKRPGGV
GVADAMFGCHRAALTKALELVEGCPCPEGCVACIHDHGCTGYNSIIDKQA
ALIVLRRLIE
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mRNA from alignment at H-elongata_contig69948:447..1518+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig69948.14640.1 ID=mRNA_H-elongata_contig69948.14640.1|Name=mRNA_H-elongata_contig69948.14640.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1072bp|location=Sequence derived from alignment at H-elongata_contig69948:447..1518+ (Himanthalia elongata Himel1 dioecious)
TTGGGGGTAAAGGGTGGAATGCACGCGGTGGGGCACGCCCTCCTGTCAGT
GGTGCCGCTCTTTCTTCTCTGCGACCCCGAGGATGTGGACTGTGAGCACG
TCAGGCCCCACAACCAGCGCCCTCAGCCGCGGCGCATCCTCGTATACGAC
AAGCGCCCCGGAGGCGTGGGCGTGGCGGACGCCATGTTCGGCTGCCACAG
GTGTGTCGGAGCGTTGACGCGTGATGTATATGACGTCACTCTTGCACGTC
GTGGCATAAAACGCCTGCCGTTCTTGGCGTAAGGGAAGGTCGCGACTCGC
TCGTGATATTGCTTGGGGTTTCTTGGAACTTGAAAAGGGTCGGAAGGTTT
CTCGTGTGCAGCAATGACCTTCGTACCTATCCCATGTCCTGAAGAAAAAG
TGGTCTTTATACTTGACTCCGCCCGAACACACGCTCTTGCTGCTCCTGCT
GTGGTCTACTCTAAGAGCCGCGCTAACCAAGGCGCTGGAGCTGGTGGAAG
GGTGTCCGTGCCCAGAGGGTTGCGTGGCATGCATTCACGACCACGGCTGC
ACAGGCTACAACTCGGTACGACGGTCGAGCGGCTCCTCCGATGCCACCGT
TTTCTTTGGACGGGGGGGAGGTGTATTCAAGGTTTACATGCCTTGGTCGT
TAGATCGCTGGCTATGCCTCCTCGGAAGGACGAAGCAGGGGGGGGTGTAC
AAAGAGTGCGATTCGCGCACACGCAGAGAGATTCTCCCCCCGCCACTCCT
GCGCGATATATCGGCTTGTATTATGGAGAGTCGTCCGCGAGCCGGGGTAA
GAAATAGTCGCACGTAGATGATACTGGGTTCGTCAGAGCTGACGAGCTGC
CCGTATAATTTTAAATTTCAAGGACACGTGCAACCGTAGACTTATTCCTT
TGCCTCCACGCCGTATTCGACCCGACCCCCCGCCCTTCATCCCACGACCC
TGTGGCTGTCACGCTTATTGTATACATAACTTCTTTCATCTTTACACGAT
CCCGTTATTACAACCGACCCCCAGATTATTGACAAGCAGGCGGCCCTGAT
CGTACTGAGGCGTCTCATTGAA
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Coding sequence (CDS) from alignment at H-elongata_contig69948:447..1518+

>mRNA_H-elongata_contig69948.14640.1 ID=mRNA_H-elongata_contig69948.14640.1|Name=mRNA_H-elongata_contig69948.14640.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=660bp|location=Sequence derived from alignment at H-elongata_contig69948:447..1518+ (Himanthalia elongata Himel1 dioecious)
ATGCACGCGGTGGGGCACGCCCTCCTGTCAGTGGTGCCGCTCTTTCTTCT
CTGCGACCCCGAGGATGTGGACTGTGAGCACGTCAGGCCCCACAACCAGC
GCCCTCAGCCGCGGCGCATCCTCGTATACGACAAGCGCCCCGGAGGCGTG
GGCGTGGCGGACGCCATGTTCGGCTGCCACAGATGCACGCGGTGGGGCAC
GCCCTCCTGTCAGTGGTGCCGCTCTTTCTTCTCTGCGACCCCGAGGATGT
GGACTGTGAGCACGTCAGGCCCCACAACCAGCGCCCTCAGCCGCGGCGCA
TCCTCGTATACGACAAGCGCCCCGGAGGCGTGGGCGTGGCGGACGCCATG
TTCGGCTGCCACAGAGCCGCGCTAACCAAGGCGCTGGAGCTGGTGGAAGG
GTGTCCGTGCCCAGAGGGTTGCGTGGCATGCATTCACGACCACGGCTGCA
CAGGCTACAACTCGAGCCGCGCTAACCAAGGCGCTGGAGCTGGTGGAAGG
GTGTCCGTGCCCAGAGGGTTGCGTGGCATGCATTCACGACCACGGCTGCA
CAGGCTACAACTCGATTATTGACAAGCAGGCGGCCCTGATCGTACTGAGG
CGTCTCATTGAAATTATTGACAAGCAGGCGGCCCTGATCGTACTGAGGCG
TCTCATTGAA
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