mRNA_H-elongata_contig68276.14454.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig68276.14454.1
Unique NamemRNA_H-elongata_contig68276.14454.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig68276.14454.1 vs. uniprot
Match: D7FPJ7_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FPJ7_ECTSI)

HSP 1 Score: 281 bits (719), Expect = 3.030e-84
Identity = 223/490 (45.51%), Postives = 274/490 (55.92%), Query Frame = 1 
Query:   13 IVREAARTSQAAFLSSVLPLQDSDVDYDLRTPIASKSEPWNTTT---------------VVRGHTRRQFSSG----SAADEADDPGTASSSVAADQDAAKIWVSLERLQDQSVGLGNAAEHQMCRALELCRDIRVQRWKVGTSASSSSEHERASRLEALESVLRGLEEVLSKRTLIDKAARRDADALGGPATIASMLDGHDVARLAAEEEDAIAA---VEVPAKTPSENCGQGEGTGWLSGAQLRAFAKQIQARSSLEISALESERRKLRIKVEMFQRQVELLQRKGAGSS-APASIE----GGSSAPVGIAARN------GLGF---ANERRFANLQNGRLREERHIKATLEAEVASLRDALEERDGLIDALREAVQSNTPTDQTGGALTSIVTEGSASDISQDEDGTPNEQGKNDTSHYVDQNDTPQDAPESSPRADAV------EQGLAVPGRGVFEPIES---QSRGRYHSRSSRLLSAAGPVSSA 1347
            IVR+AARTSQAAFLSSVLP QD D D    T  +  S P+  TT               V  G   R    G    S    A   G+     ++D  AA  W S+ RL+DQ  GLGNAA+HQ+ RAL+LCRD+R Q  K G    S S H RASRL+AL+S L GLEEVLSKR ++DK AR +A+ALGGPA   S+L+  D   +  +++  +AA                     GWL+G+QL+AF K+++ARSS+EISALESERRKLR KVEMFQRQV+LLQR+GAG S APA+      GG  A    AA        G GF   A E+RFA+L + RL EER ++ATLEAE+ASLR  +EE+DGL+ ALREA++S               T  S  D S    GT  E G                 P     AD +      +    VPGRGVF P  S   +SRGRYHSRSSR LSA GPV++A
Sbjct:   35 IVRQAARTSQAAFLSSVLPTQDLDFD----TANSDSSNPFTPTTLPGISLPNGRHRSPVVGPGEPGRAAGLGGGGVSGLGAAISAGSGVGPTSSDTQAAARWASMRRLEDQGAGLGNAADHQLARALQLCRDLRGQ--KAGVGGGSLSVHGRASRLDALQSTLHGLEEVLSKRRVVDKTARAEAEALGGPAAAWSLLND-DEGSVGDKDQTQVAASGRXXXXXXXXXXXXXXXXSGGWLTGSQLKAFEKKVEARSSIEISALESERRKLRSKVEMFQRQVQLLQREGAGHSPAPANGSTGKIGGYPAATAAAATGEGDSGVGGGFGAAAGEKRFADLLDARLGEERIVQATLEAELASLRRTIEEKDGLVGALREALRS-------------AATPASGGDASTARAGTLAEHGA-------------AQPPARRRSADGILTPRSADGRAVVPGRGVFSPQSSDSSRSRGRYHSRSSRFLSAQGPVAAA 491
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BLAST of mRNA_H-elongata_contig68276.14454.1 vs. uniprot
Match: A0A6H5KLE5_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KLE5_9PHAE)

HSP 1 Score: 276 bits (705), Expect = 3.050e-80
Identity = 215/488 (44.06%), Postives = 277/488 (56.76%), Query Frame = 1 
Query:    1 PALQIVREAARTSQAAFLSSVLPLQDSDVDYDL---------------------RTPIASKSEPWNTTTVVRGHTRRQFSSGSAADEADDPGTASSSVAADQDAAKIWVSLERLQDQSVGLGNAAEHQMCRALELCRDIRVQRWKVGTSASSSSEHERASRLEALESVLRGLEEVLSKRTLIDKAARRDADALGGPATIASMLDGHDVARLAAEEEDAIAAV--EVPAKTPSENCGQGEGTGWLSGAQLRAFAKQIQARSSLEISALESERRKLRIKVEMFQRQVELLQRKGAGSS-APASIEGGSSA--PVGIAAR-----NGLG-----FANERRFANLQNGRLREERHIKATLEAEVASLRDALEERDGLIDALREAVQSNTPTDQTGGALTSIVTEGSASDISQDEDGTPNEQGKNDTSHYVDQNDTPQDAPESSPRADAVEQGLAVPGRGVFEPIESQSR---GRYHSRSSRLLSAAGPVSSA 1347
            P  +IVR+AARTSQAAFLSSVLP Q+ D D                        R+P+    EP   + +  G        G+A    +  G  SS    D  AA  W S+ RL+DQ  GLGNAA+HQ+ RAL+LCRD+R    K G    S S HERASRL+AL+S L GLEEVLSKR ++D+ AR +A+ALGGPA++ S+L+G + +    ++    ++   E  A   +   G G G GWL+G+QL+AF K ++AR+S+EISALESERRKLR KVEMFQRQV+LLQR GAG S APA+   G     P   AA      NG G      A E+RFA+L + RL EER ++ATLEAE+ASLR  +EE+DGLI ALREA++S     + G A T+               GT          H   Q+   + + +      + +    VP RGV  P  S S    GRYHSRSSR LSA GPV++A
Sbjct:  275 PTPEIVRQAARTSQAAFLSSVLPTQNLDFDTANSDSSNPFTRAALPESPPNGRHRSPVVGPGEPGRASGIGGGGVSGL---GAAISSGNGVGLTSS----DTRAAARWASMRRLEDQGAGLGNAADHQLARALQLCRDLRGL--KAGMGGGSQSGHERASRLDALQSTLHGLEEVLSKRRVVDQTARAEAEALGGPASVWSLLNGDEGSGTDKDQTQVSSSGRREGAATGTTTAIGGGSG-GWLTGSQLKAFEKNVEARTSIEISALESERRKLRSKVEMFQRQVQLLQRDGAGHSPAPANGSTGKIGGYPAAAAAATSEVDNGAGGRFGAAAGEKRFADLLDARLGEERIVQATLEAELASLRRTIEEKDGLIGALREALRSAATPARGGDAATARA-------------GTL-------AGHGAAQSSARRRSADGMSTPRSADDHAVVPTRGVLLPRSSDSSRSLGRYHSRSSRFLSAQGPVAAA 732
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig68276.14454.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
D7FPJ7_ECTSI3.030e-8445.51Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5KLE5_9PHAE3.050e-8044.06Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig68276contigH-elongata_contig68276:393..2843 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionexpressed unknown protein
Ec32 orthologEc-12_000840.1
Exons5
Model size1359
Cds size1359
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig68276.14454.1prot_H-elongata_contig68276.14454.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig68276 393..2843 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931076.1238043-CDS-H-elongata_contig68276:392..7791622931076.1238043-CDS-H-elongata_contig68276:392..779Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig68276 393..779 +
1691679688.0420654-CDS-H-elongata_contig68276:392..7791691679688.0420654-CDS-H-elongata_contig68276:392..779Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig68276 393..779 +
1622931076.1386855-CDS-H-elongata_contig68276:1015..13351622931076.1386855-CDS-H-elongata_contig68276:1015..1335Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig68276 1016..1335 +
1691679688.0550332-CDS-H-elongata_contig68276:1015..13351691679688.0550332-CDS-H-elongata_contig68276:1015..1335Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig68276 1016..1335 +
1622931076.152395-CDS-H-elongata_contig68276:1550..16261622931076.152395-CDS-H-elongata_contig68276:1550..1626Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig68276 1551..1626 +
1691679688.063027-CDS-H-elongata_contig68276:1550..16261691679688.063027-CDS-H-elongata_contig68276:1550..1626Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig68276 1551..1626 +
1622931076.170062-CDS-H-elongata_contig68276:1843..20111622931076.170062-CDS-H-elongata_contig68276:1843..2011Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig68276 1844..2011 +
1691679688.0707364-CDS-H-elongata_contig68276:1843..20111691679688.0707364-CDS-H-elongata_contig68276:1843..2011Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig68276 1844..2011 +
1622931076.1930923-CDS-H-elongata_contig68276:2435..28431622931076.1930923-CDS-H-elongata_contig68276:2435..2843Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig68276 2436..2843 +
1691679688.07852-CDS-H-elongata_contig68276:2435..28431691679688.07852-CDS-H-elongata_contig68276:2435..2843Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig68276 2436..2843 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig68276.14454.1

>prot_H-elongata_contig68276.14454.1 ID=prot_H-elongata_contig68276.14454.1|Name=mRNA_H-elongata_contig68276.14454.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=453bp
PALQIVREAARTSQAAFLSSVLPLQDSDVDYDLRTPIASKSEPWNTTTVV
RGHTRRQFSSGSAADEADDPGTASSSVAADQDAAKIWVSLERLQDQSVGL
GNAAEHQMCRALELCRDIRVQRWKVGTSASSSSEHERASRLEALESVLRG
LEEVLSKRTLIDKAARRDADALGGPATIASMLDGHDVARLAAEEEDAIAA
VEVPAKTPSENCGQGEGTGWLSGAQLRAFAKQIQARSSLEISALESERRK
LRIKVEMFQRQVELLQRKGAGSSAPASIEGGSSAPVGIAARNGLGFANER
RFANLQNGRLREERHIKATLEAEVASLRDALEERDGLIDALREAVQSNTP
TDQTGGALTSIVTEGSASDISQDEDGTPNEQGKNDTSHYVDQNDTPQDAP
ESSPRADAVEQGLAVPGRGVFEPIESQSRGRYHSRSSRLLSAAGPVSSAL
AGA
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mRNA from alignment at H-elongata_contig68276:393..2843+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig68276.14454.1 ID=mRNA_H-elongata_contig68276.14454.1|Name=mRNA_H-elongata_contig68276.14454.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=2451bp|location=Sequence derived from alignment at H-elongata_contig68276:393..2843+ (Himanthalia elongata Himel1 dioecious)
CCTGCCCTGCAGATAGTGCGAGAGGCGGCGCGTACTTCCCAGGCGGCTTT
TCTCTCTTCGGTTCTCCCGTTACAGGATTCCGATGTGGACTATGACCTTA
GAACCCCTATAGCCTCAAAGTCTGAACCCTGGAACACCACGACTGTTGTA
AGGGGGCATACTCGGCGACAATTCAGCAGTGGATCGGCCGCGGACGAAGC
CGACGATCCCGGCACCGCTTCCAGTAGCGTCGCCGCAGACCAAGATGCGG
CAAAAATATGGGTCTCGCTGGAAAGGCTCCAAGATCAAAGCGTTGGACTC
GGTAACGCCGCGGAGCATCAAATGTGTCGTGCGCTCGAGCTCTGTCGCGA
TATCCGAGTCCAACGCTGGAAGGTAGGCACCTCGGCTGTCAATGCCAACA
TGGATGTATTTTGCACTTAAGAATGTTGGTGTGAATCAAATTTATGGCGT
GTACGCGCTGCCATCTTGGAACGCTCGCAAGCTTTTCGACTTTAGTTGTC
TTCCTCGGATGTGGCATTGTATGGTGTATATAATACCTATATTATATAAT
GGTATGGTATATAATTGTTTCGCATCGATCATTCTTTGTTTCCCTGCAGG
CAGGGGTCGGGGCTGCCAGCCAGAGTTCATCTTCCGAACACGAACGTGCG
TCTCGGCTGGAGGCTCTTGAGTCGGTCTTGCGGGGCCTGGAAGAAGTTTT
GTCGAAGCGAACCCTCATTGACAAGGCCGCCAGACGCGACGCGGATGCTC
TTGGTGGCCCGGCAACGATTGCGAGCATGTTGGACGGTCACGACGTGGCT
CGCCTAGCGGCCGAGGAGGAAGACGCGATTGCGGCGGTCGAAGTACCGGC
CAAGACACCCTCAGAGAATTGCGGCCAGGGAGAGGGCACTGGGTGGCTGA
GCGGTGCTCAGCTGAGGGCCTTCGCAAAGCAGATCCAGGCTAGGTACTAG
ATATTGGCACGCCCGTGTGAAGTACACCCATGTACACGGAGATATAGCTG
TAAATGATTGTGTATCGTGTGGTGAGATATTGCGACGGGAAAGCGAGTGG
TCGTGCGCCTGTATAATCATGTCTTATAATAAAAGCACCGTTTGTCATGA
GCGATGGTAGACCTTCTATGCGATCATGTTGCGCTACTCTGCTCCAATAC
TAAACCAGGTCCTCCTTAGAAATATCGGCTCTTGAGTCCGAGAGGCGCAA
GCTAAGAATTAAAGTGGAGATGTTCCAGAGGCAGGTGAGATGTACCGTCT
TCCCTAGGCACGGATGGGCGTGTATCGTGATCCCCGGACGTGGTTTCTAA
TATCTGCTTGGGGTTGAATTAGTTGTTTTACGCAGCAGTACATCCTAATC
ATGTCGTCGGGACCATACGCCACCCTGCCGTTAAAATCTTAGAAAAGTTG
CTTGAATCATTTCAACATGGTGGCCATATTTCCATTTCCGTCCGAGCCCA
GGTGGAGCTTTTGCAGCGCAAAGGAGCCGGGTCCTCTGCTCCAGCGTCCA
TCGAGGGTGGGAGTAGCGCCCCCGTTGGTATTGCTGCGCGCAACGGGTTA
GGGTTCGCCAACGAAAGGCGGTTCGCTAATCTCCAAAACGGTCGCTTGAG
AGAGGAACGTCACATCAAGGTGATTTACTTCTCCGTCTTGGCGGCAGAGC
GATACATCGCCGTTCTGACGACCTCGTCGATGACCGGGATAATCTGGTGC
ACGCAATGCTCTAGTGTTTTTATTTTTTTAATTTCGAACTCATTCCAACC
CTCCCAACACCCACACCTTCTTAGCGGCGTGAGAGGTGCAGTCCAGGGGA
CCAACTATCGTTAGATTCGAATGTGTACACGTGAACAAGGCGCTGTACGA
CTGTCTGGAAAAATTTTGAAGTGCCGCAGTGTCGGCCGCGTTCGCCTCAA
ACATAAACATTCGTTGTGCGTGTCAGATTAATAATGTCTCCGTTTTAATT
AATAATCTCTCCGTGTTATGTCCCAAGTAACATCAATCCCCGCGTAGAAA
TGATACGACGTTGAGAGCTAACAATGTGTCGTGAACTCCGCAGGCGACCC
TCGAAGCGGAAGTGGCTTCGCTTCGCGATGCACTAGAGGAAAGAGACGGA
CTCATCGATGCGCTTCGCGAGGCCGTCCAGTCGAACACACCGACGGATCA
GACCGGCGGTGCCCTCACCTCGATCGTTACTGAGGGTTCTGCTTCCGATA
TTTCCCAAGACGAAGATGGCACCCCCAATGAACAAGGAAAAAACGATACT
TCGCACTACGTGGACCAAAACGACACCCCCCAAGACGCCCCAGAGAGCTC
CCCTCGGGCCGACGCCGTCGAACAGGGCCTGGCCGTGCCGGGACGTGGAG
TTTTTGAACCTATCGAGAGCCAGAGTCGCGGACGCTACCACTCGAGATCG
TCCCGACTGCTCAGTGCCGCTGGACCGGTGTCGAGCGCGTTAGCAGGAGC
G
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Coding sequence (CDS) from alignment at H-elongata_contig68276:393..2843+

>mRNA_H-elongata_contig68276.14454.1 ID=mRNA_H-elongata_contig68276.14454.1|Name=mRNA_H-elongata_contig68276.14454.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=2718bp|location=Sequence derived from alignment at H-elongata_contig68276:393..2843+ (Himanthalia elongata Himel1 dioecious)
CCTGCCCTGCAGATAGTGCGAGAGGCGGCGCGTACTTCCCAGGCGGCTTT
TCTCTCTTCGGTTCTCCCGTTACAGGATTCCGATGTGGACTATGACCTTA
GAACCCCTATAGCCTCAAAGTCTGAACCCTGGAACACCACGACTGTTGTA
AGGGGGCATACTCGGCGACAATTCAGCAGTGGATCGGCCGCGGACGAAGC
CGACGATCCCGGCACCGCTTCCAGTAGCGTCGCCGCAGACCAAGATGCGG
CAAAAATATGGGTCTCGCTGGAAAGGCTCCAAGATCAAAGCGTTGGACTC
GGTAACGCCGCGGAGCATCAAATGTGTCGTGCGCTCGAGCTCTGTCGCGA
TATCCGAGTCCAACGCTGGAAGGTAGGCACCTCGGCTCCTGCCCTGCAGA
TAGTGCGAGAGGCGGCGCGTACTTCCCAGGCGGCTTTTCTCTCTTCGGTT
CTCCCGTTACAGGATTCCGATGTGGACTATGACCTTAGAACCCCTATAGC
CTCAAAGTCTGAACCCTGGAACACCACGACTGTTGTAAGGGGGCATACTC
GGCGACAATTCAGCAGTGGATCGGCCGCGGACGAAGCCGACGATCCCGGC
ACCGCTTCCAGTAGCGTCGCCGCAGACCAAGATGCGGCAAAAATATGGGT
CTCGCTGGAAAGGCTCCAAGATCAAAGCGTTGGACTCGGTAACGCCGCGG
AGCATCAAATGTGTCGTGCGCTCGAGCTCTGTCGCGATATCCGAGTCCAA
CGCTGGAAGGTAGGCACCTCGGCTAGTTCATCTTCCGAACACGAACGTGC
GTCTCGGCTGGAGGCTCTTGAGTCGGTCTTGCGGGGCCTGGAAGAAGTTT
TGTCGAAGCGAACCCTCATTGACAAGGCCGCCAGACGCGACGCGGATGCT
CTTGGTGGCCCGGCAACGATTGCGAGCATGTTGGACGGTCACGACGTGGC
TCGCCTAGCGGCCGAGGAGGAAGACGCGATTGCGGCGGTCGAAGTACCGG
CCAAGACACCCTCAGAGAATTGCGGCCAGGGAGAGGGCACTGGGTGGCTG
AGCGGTGCTCAGCTGAGGGCCTTCGCAAAGCAGATCCAGGCTAGAGTTCA
TCTTCCGAACACGAACGTGCGTCTCGGCTGGAGGCTCTTGAGTCGGTCTT
GCGGGGCCTGGAAGAAGTTTTGTCGAAGCGAACCCTCATTGACAAGGCCG
CCAGACGCGACGCGGATGCTCTTGGTGGCCCGGCAACGATTGCGAGCATG
TTGGACGGTCACGACGTGGCTCGCCTAGCGGCCGAGGAGGAAGACGCGAT
TGCGGCGGTCGAAGTACCGGCCAAGACACCCTCAGAGAATTGCGGCCAGG
GAGAGGGCACTGGGTGGCTGAGCGGTGCTCAGCTGAGGGCCTTCGCAAAG
CAGATCCAGGCTAGGTCCTCCTTAGAAATATCGGCTCTTGAGTCCGAGAG
GCGCAAGCTAAGAATTAAAGTGGAGATGTTCCAGAGGCAGGTCCTCCTTA
GAAATATCGGCTCTTGAGTCCGAGAGGCGCAAGCTAAGAATTAAAGTGGA
GATGTTCCAGAGGCAGGTGGAGCTTTTGCAGCGCAAAGGAGCCGGGTCCT
CTGCTCCAGCGTCCATCGAGGGTGGGAGTAGCGCCCCCGTTGGTATTGCT
GCGCGCAACGGGTTAGGGTTCGCCAACGAAAGGCGGTTCGCTAATCTCCA
AAACGGTCGCTTGAGAGAGGAACGTCACATCAAGGTGGAGCTTTTGCAGC
GCAAAGGAGCCGGGTCCTCTGCTCCAGCGTCCATCGAGGGTGGGAGTAGC
GCCCCCGTTGGTATTGCTGCGCGCAACGGGTTAGGGTTCGCCAACGAAAG
GCGGTTCGCTAATCTCCAAAACGGTCGCTTGAGAGAGGAACGTCACATCA
AGGCGACCCTCGAAGCGGAAGTGGCTTCGCTTCGCGATGCACTAGAGGAA
AGAGACGGACTCATCGATGCGCTTCGCGAGGCCGTCCAGTCGAACACACC
GACGGATCAGACCGGCGGTGCCCTCACCTCGATCGTTACTGAGGGTTCTG
CTTCCGATATTTCCCAAGACGAAGATGGCACCCCCAATGAACAAGGAAAA
AACGATACTTCGCACTACGTGGACCAAAACGACACCCCCCAAGACGCCCC
AGAGAGCTCCCCTCGGGCCGACGCCGTCGAACAGGGCCTGGCCGTGCCGG
GACGTGGAGTTTTTGAACCTATCGAGAGCCAGAGTCGCGGACGCTACCAC
TCGAGATCGTCCCGACTGCTCAGTGCCGCTGGACCGGTGTCGAGCGCGTT
AGCAGGAGCGGCGACCCTCGAAGCGGAAGTGGCTTCGCTTCGCGATGCAC
TAGAGGAAAGAGACGGACTCATCGATGCGCTTCGCGAGGCCGTCCAGTCG
AACACACCGACGGATCAGACCGGCGGTGCCCTCACCTCGATCGTTACTGA
GGGTTCTGCTTCCGATATTTCCCAAGACGAAGATGGCACCCCCAATGAAC
AAGGAAAAAACGATACTTCGCACTACGTGGACCAAAACGACACCCCCCAA
GACGCCCCAGAGAGCTCCCCTCGGGCCGACGCCGTCGAACAGGGCCTGGC
CGTGCCGGGACGTGGAGTTTTTGAACCTATCGAGAGCCAGAGTCGCGGAC
GCTACCACTCGAGATCGTCCCGACTGCTCAGTGCCGCTGGACCGGTGTCG
AGCGCGTTAGCAGGAGCG
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