mRNA_H-elongata_contig66198.14213.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig66198.14213.1
Unique NamemRNA_H-elongata_contig66198.14213.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig66198.14213.1 vs. uniprot
Match: D7FJM8_ECTSI (DEAD box helicase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FJM8_ECTSI)

HSP 1 Score: 160 bits (406), Expect = 8.380e-44
Identity = 82/94 (87.23%), Postives = 87/94 (92.55%), Query Frame = 1 
Query:    1 GVHTVINFEMPRSADSYIHRVGRTARAGCGGCSVTLIGESRRIVMKEVLKTQGCEVEIKSRAVPQSAIDHYRSKIEGLESSVKDIFAEEKVRRD 282
            GVHTVINFEMPRSADSYIHRVGRTARAGCGG SVTLIGESRRIVMKEVLKTQG  VEIKSRAVPQSAIDH+RSKIEG+E+SVK I  EEKV ++
Sbjct:  621 GVHTVINFEMPRSADSYIHRVGRTARAGCGGRSVTLIGESRRIVMKEVLKTQGEGVEIKSRAVPQSAIDHFRSKIEGMEASVKAILTEEKVEKE 714
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BLAST of mRNA_H-elongata_contig66198.14213.1 vs. uniprot
Match: A0A835YJC4_9STRA (P-loop containing nucleoside triphosphate hydrolase protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YJC4_9STRA)

HSP 1 Score: 139 bits (351), Expect = 2.270e-36
Identity = 69/94 (73.40%), Postives = 79/94 (84.04%), Query Frame = 1 
Query:    1 GVHTVINFEMPRSADSYIHRVGRTARAGCGGCSVTLIGESRRIVMKEVLKTQGCEVEIKSRAVPQSAIDHYRSKIEGLESSVKDIFAEEKVRRD 282
            GVHTVIN+EMPRS D+YIHRVGRTARAGCGG SVTLIGESRR+VMK+VLK Q  + E+KSRAVPQS IDHYRSKI+ LE  V  I  EEK+ ++
Sbjct:  599 GVHTVINYEMPRSRDTYIHRVGRTARAGCGGRSVTLIGESRRLVMKDVLKIQAEKAEVKSRAVPQSVIDHYRSKIDMLEDEVAAIMREEKLEKE 692
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BLAST of mRNA_H-elongata_contig66198.14213.1 vs. uniprot
Match: A0A7S2V390_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V390_9STRA)

HSP 1 Score: 98.2 bits (243), Expect = 8.480e-22
Identity = 52/106 (49.06%), Postives = 68/106 (64.15%), Query Frame = 1 
Query:    1 GVHTVINFEMPRSADSYIHRVGRTARAGCGGCSVTLIGESRRIVMKEVLKTQGCEVE------------IKSRAVPQSAIDHYRSKIEGLESSVKDIFAEEKVRRD 282
            GV TVIN +MPR+  +YIHRVGR+ARAG  G +VTL+GE RR VMKEVL+                   +KSR VPQS +DH+R KI+ LE  +K I  EE++ ++
Sbjct:  372 GVRTVINLDMPRTRTTYIHRVGRSARAGKSGRAVTLVGEGRRAVMKEVLRHHXXXXXXXXXXXXXXXSMMKSRTVPQSVVDHFRLKIKALEPEIKKILDEEQMEKE 477
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BLAST of mRNA_H-elongata_contig66198.14213.1 vs. uniprot
Match: A0A8K1CFP2_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CFP2_PYTOL)

HSP 1 Score: 98.2 bits (243), Expect = 8.600e-22
Identity = 48/93 (51.61%), Postives = 67/93 (72.04%), Query Frame = 1 
Query:    1 GVHTVINFEMPRSADSYIHRVGRTARAGCGGCSVTLIGESRRIVMKEVLKTQGCEVEIKSRAVPQSAIDHYRSKIEGLESSVKDIFAEEKVRR 279
            GVH VIN+EMP+   +Y+HRVGRTARAG  G +VTL  ESRR++MK+V  ++ C   +KSRAVP   I  ++++IEG+E+ +K I  EE + R
Sbjct:  565 GVHAVINYEMPKDLTTYVHRVGRTARAGRNGRAVTLTSESRRLLMKQV--SRHCHGFVKSRAVPDPVIAQWKARIEGMENDIKQIMHEETLER 655
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BLAST of mRNA_H-elongata_contig66198.14213.1 vs. uniprot
Match: A0A662YB28_9STRA (Uncharacterized protein n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662YB28_9STRA)

HSP 1 Score: 97.8 bits (242), Expect = 1.170e-21
Identity = 47/93 (50.54%), Postives = 67/93 (72.04%), Query Frame = 1 
Query:    1 GVHTVINFEMPRSADSYIHRVGRTARAGCGGCSVTLIGESRRIVMKEVLKTQGCEVEIKSRAVPQSAIDHYRSKIEGLESSVKDIFAEEKVRR 279
            GVH VIN+EMP+   +Y+HRVGRTARAG  G +VTL  ESRR+VMK+V  ++ C+  +KSRAVP   I  ++++IEG++  VK +  EE + +
Sbjct:  564 GVHAVINYEMPKDITTYVHRVGRTARAGRNGRAVTLTSESRRLVMKQV--SRHCQGFVKSRAVPDPVITQWKARIEGMQEDVKQVMHEETLEK 654
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BLAST of mRNA_H-elongata_contig66198.14213.1 vs. uniprot
Match: UPI000A2A8E74 (probable ATP-dependent RNA helicase DDX27 n=1 Tax=Exaiptasia diaphana TaxID=2652724 RepID=UPI000A2A8E74)

HSP 1 Score: 95.9 bits (237), Expect = 5.390e-21
Identity = 47/94 (50.00%), Postives = 62/94 (65.96%), Query Frame = 1 
Query:    1 GVHTVINFEMPRSADSYIHRVGRTARAGCGGCSVTLIGESRRIVMKEVLKTQGCEVEIKSRAVPQSAIDHYRSKIEGLESSVKDIFAEEKVRRD 282
            GV TVINF MP +   Y+HRVGRTARAG  G SVTL+GE  R  +KEV+K      ++KSR +P   I+ Y SK+E LE+ +KDI  +E   ++
Sbjct:  367 GVKTVINFSMPTNVKQYVHRVGRTARAGKSGRSVTLVGEKERKSLKEVVKNS--RTQVKSRIIPPEVIEKYSSKVEALEADIKDILKQENEEKE 458
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BLAST of mRNA_H-elongata_contig66198.14213.1 vs. uniprot
Match: A0A484EDS2_BRELC (Uncharacterized protein n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484EDS2_BRELC)

HSP 1 Score: 95.9 bits (237), Expect = 5.550e-21
Identity = 46/93 (49.46%), Postives = 67/93 (72.04%), Query Frame = 1 
Query:    1 GVHTVINFEMPRSADSYIHRVGRTARAGCGGCSVTLIGESRRIVMKEVLKTQGCEVEIKSRAVPQSAIDHYRSKIEGLESSVKDIFAEEKVRR 279
            GVH VIN+EMP+   +Y+HRVGRTARAG  G +VTL  ESRR+VMK+V  ++ C+  +KSRAVP   I  ++++IE ++  VK++  EE + +
Sbjct:  532 GVHAVINYEMPKDITTYVHRVGRTARAGRNGRAVTLTSESRRMVMKQV--SRHCQGFVKSRAVPDPVITQWKARIESMQEDVKEVMHEETLEK 622
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BLAST of mRNA_H-elongata_contig66198.14213.1 vs. uniprot
Match: A0A0P1AUZ9_PLAHL (Dimethyladenosine transferase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1AUZ9_PLAHL)

HSP 1 Score: 95.9 bits (237), Expect = 5.630e-21
Identity = 47/93 (50.54%), Postives = 66/93 (70.97%), Query Frame = 1 
Query:    1 GVHTVINFEMPRSADSYIHRVGRTARAGCGGCSVTLIGESRRIVMKEVLKTQGCEVEIKSRAVPQSAIDHYRSKIEGLESSVKDIFAEEKVRR 279
            GVH VIN+EMP+   +Y+HRVGRTARAG  G SVTL  ESRR+VMK+V  ++ C+  +KSRAVP   I  ++++IE +E  +K +  EE + +
Sbjct:  521 GVHAVINYEMPKDITTYVHRVGRTARAGRNGRSVTLTSESRRLVMKQV--SRHCQGFVKSRAVPDPVIAQWKARIESMEEDIKMVMHEETLEK 611
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BLAST of mRNA_H-elongata_contig66198.14213.1 vs. uniprot
Match: A0A024GL98_9STRA (Uncharacterized protein n=1 Tax=Albugo candida TaxID=65357 RepID=A0A024GL98_9STRA)

HSP 1 Score: 94.7 bits (234), Expect = 1.410e-20
Identity = 47/93 (50.54%), Postives = 65/93 (69.89%), Query Frame = 1 
Query:    1 GVHTVINFEMPRSADSYIHRVGRTARAGCGGCSVTLIGESRRIVMKEVLKTQGCEVEIKSRAVPQSAIDHYRSKIEGLESSVKDIFAEEKVRR 279
            GVH VIN+EMP+   +Y+HRVGRTARAG  G +VTL  ESRR++MK+V  T+ C    K+RAVP + I  +++KI+ L+  +  I  EEKV +
Sbjct:  530 GVHAVINYEMPKDITTYVHRVGRTARAGQVGRAVTLTSESRRLIMKQV--TRHCHGFAKARAVPDTVITQWKAKIQSLQQDIAHILMEEKVEK 620
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BLAST of mRNA_H-elongata_contig66198.14213.1 vs. uniprot
Match: A0A6G0X8Y5_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0X8Y5_9STRA)

HSP 1 Score: 94.4 bits (233), Expect = 1.920e-20
Identity = 44/93 (47.31%), Postives = 64/93 (68.82%), Query Frame = 1 
Query:    1 GVHTVINFEMPRSADSYIHRVGRTARAGCGGCSVTLIGESRRIVMKEVLKTQGCEVEIKSRAVPQSAIDHYRSKIEGLESSVKDIFAEEKVRR 279
            GVH VIN++MP+   +Y+HRVGRTARAG  G +VTL  E+RR++MKEV +   C   +KSR+VP + I  ++ +IE +E  VK +  EE + +
Sbjct:  513 GVHAVINYQMPKDVTTYVHRVGRTARAGRNGRAVTLTSENRRLIMKEVARH--CHGFVKSRSVPDAVIAQWKKRIESMEDDVKSVMKEETIEK 603
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig66198.14213.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FJM8_ECTSI8.380e-4487.23DEAD box helicase n=2 Tax=Ectocarpus TaxID=2879 Re... [more]
A0A835YJC4_9STRA2.270e-3673.40P-loop containing nucleoside triphosphate hydrolas... [more]
A0A7S2V390_9STRA8.480e-2249.06Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A8K1CFP2_PYTOL8.600e-2251.61Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A662YB28_9STRA1.170e-2150.54Uncharacterized protein n=2 Tax=Nothophytophthora ... [more]
UPI000A2A8E745.390e-2150.00probable ATP-dependent RNA helicase DDX27 n=1 Tax=... [more]
A0A484EDS2_BRELC5.550e-2149.46Uncharacterized protein n=1 Tax=Bremia lactucae Ta... [more]
A0A0P1AUZ9_PLAHL5.630e-2150.54Dimethyladenosine transferase n=1 Tax=Plasmopara h... [more]
A0A024GL98_9STRA1.410e-2050.54Uncharacterized protein n=1 Tax=Albugo candida Tax... [more]
A0A6G0X8Y5_9STRA1.920e-2047.31Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig66198contigH-elongata_contig66198:1108..2805 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score143.3
Seed ortholog evalue5.5e-32
Seed eggNOG ortholog2880.D7FJM8
Preferred nameDDX27
KEGG koko:K13181
GOsGO:0000027,GO:0000166,GO:0003674,GO:0003676,GO:0003723,GO:0003724,GO:0003824,GO:0004004,GO:0004386,GO:0005488,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005694,GO:0005730,GO:0005737,GO:0006139,GO:0006364,GO:0006396,GO:0006725,GO:0006807,GO:0006996,GO:0008026,GO:0008144,GO:0008150,GO:0008152,GO:0008186,GO:0009987,GO:0010467,GO:0010501,GO:0016043,GO:0016070,GO:0016072,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017076,GO:0017111,GO:0022607,GO:0022613,GO:0022618,GO:0030554,GO:0030684,GO:0030687,GO:0031974,GO:0031981,GO:0032553,GO:0032555,GO:0032559,GO:0032991,GO:0034470,GO:0034622,GO:0034641,GO:0034660,GO:0035639,GO:0035690,GO:0036094,GO:0042221,GO:0042254,GO:0042255,GO:0042273,GO:0042493,GO:0042623,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0046483,GO:0050896,GO:0051716,GO:0065003,GO:0070013,GO:0070035,GO:0070887,GO:0070925,GO:0071704,GO:0071826,GO:0071840,GO:0090304,GO:0097159,GO:0097367,GO:0140098,GO:1901265,GO:1901360,GO:1901363,GO:1990904
EggNOG free text desc.helicase activity
EggNOG OGsCOG0513@1,KOG0338@2759
Ec32 ortholog descriptionDEAD box helicase
Ec32 orthologEc-09_000390.1
EC3.6.4.13
COG Functional cat.JKL
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03009,ko03041
Hectar predicted targeting categoryother localisation
Exons3
Model size294
Cds size267
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig66198.14213.1prot_H-elongata_contig66198.14213.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig66198 1135..2805 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931050.6498206-UTR-H-elongata_contig66198:1107..11341622931050.6498206-UTR-H-elongata_contig66198:1107..1134Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig66198 1108..1134 +
1691679677.7115047-UTR-H-elongata_contig66198:1107..11341691679677.7115047-UTR-H-elongata_contig66198:1107..1134Himanthalia elongata Himel1 dioeciousUTRH-elongata_contig66198 1108..1134 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931050.6700299-CDS-H-elongata_contig66198:1134..12191622931050.6700299-CDS-H-elongata_contig66198:1134..1219Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig66198 1135..1219 +
1691679677.7246237-CDS-H-elongata_contig66198:1134..12191691679677.7246237-CDS-H-elongata_contig66198:1134..1219Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig66198 1135..1219 +
1622931050.6889517-CDS-H-elongata_contig66198:1800..19031622931050.6889517-CDS-H-elongata_contig66198:1800..1903Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig66198 1801..1903 +
1691679677.7357476-CDS-H-elongata_contig66198:1800..19031691679677.7357476-CDS-H-elongata_contig66198:1800..1903Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig66198 1801..1903 +
1622931050.704239-CDS-H-elongata_contig66198:2726..28051622931050.704239-CDS-H-elongata_contig66198:2726..2805Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig66198 2727..2805 +
1691679677.746088-CDS-H-elongata_contig66198:2726..28051691679677.746088-CDS-H-elongata_contig66198:2726..2805Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig66198 2727..2805 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig66198.14213.1

>prot_H-elongata_contig66198.14213.1 ID=prot_H-elongata_contig66198.14213.1|Name=mRNA_H-elongata_contig66198.14213.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=89bp
MPRSADSYIHRVGRTARAGCGGCSVTLIGESRRIVMKEVLKTQGCEVEIK
SRAVPQSAIDHYRSKIEGLESSVKDIFAEEKVRRDNYA*
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mRNA from alignment at H-elongata_contig66198:1108..2805+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig66198.14213.1 ID=mRNA_H-elongata_contig66198.14213.1|Name=mRNA_H-elongata_contig66198.14213.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1698bp|location=Sequence derived from alignment at H-elongata_contig66198:1108..2805+ (Himanthalia elongata Himel1 dioecious)
GGGGTCCATACCGTGATCAACTTCGAGATGCCGCGCTCCGCGGATTCGTA
CATCCACCGAGTCGGCCGGACGGCGAGGGCGGGCTGCGGAGGGTGCTCGG
TGACACTTATCGGTGAGAGATGACGCGAACAAACTCTAAAAACGATATAT
AATTTGAAAAGACTTTTTTTTTACTCCAGGATCTCGCGATATGGTACGGA
ATGTGGCCAGGATCCGCATCAGTGTTTTGACCCCTATGGAACCTCGATAT
TTTTTTAGACACTAAATTCAACACTATAAAACTTGAGAGTTGGAATGAGC
CTAAAGTCTTTTATAAATGAAAAGGGTTAAACTGGGAGGGGGATTAGTAG
ATTTATATTCCTTTTGTGATTTTTTTATTCGATTGCCTTCTGTTGATTCT
GTACGTATAACACATCTATTTGTCTGTCTGTATATAACACATCGAAACAA
GGCATTTTAATCCCTTTTTGTGTGTCGTTTCTAGCATATAGGTTATGTCG
TTATGTCGTCATCACCTGCAGCAGAGTAAAAAGCAACCCAATGATCCAAG
AATGCTACCCCCCCTCCGCCCCCGCTTCTCATTCCTTCCCCTGTCCCCTG
TTTCACGCGAAAGGGCGATATTGCATTGCGAACAATTTTGACATTCCGGT
TTTTGACGGCCCGTAATTTTGCTTTGATTATTTTGGCACACAGGCGAAAG
CAGACGGATAGTGATGAAAGAGGTGCTAAAAACGCAGGGGTGCGAGGTGG
AGATCAAGAGTAGGGCGGTCCCCCAGTCGGCCATTGACCACTACAGGTAC
ATGTGGTATGTCTTGTCTCCATAAACAACGTGGATGTCCCCGAATAGAGT
ATCTCAGACGTTTTGATGTCAAGGTTACGGTCAAGGCAAAGCAGGGTGGC
ACGTGGCGACACAATCACGCGAAATGTCTGCGCAGTCGTAGGTCCATGCC
CACGTTGGATAGCGCGTGATTGATTTTTTTTTGTCTCTTTTATCTCTTTT
GTTTTGTCTCCCGGCCAATGGTTGATGGTTAATGACCAGTGGTTGATGGC
TAATAACCAATGGTTGATGGCTAATGGTTATTAATTGATGGCTGGTGGTC
TCAAGTGGCTGCTGACGGTGCCAAGGTGATCATGATACGTACGGTGTGAT
CTGGTTACGCGCTGGGGTTATGACGAAAAAAAGTTTCGCCCGCGAAAAAC
TACAATCGAATTATCTCTAACCAATCTATCTCGCTTTACTCAAGTTCTTT
TTGAGAGATAAAATATTGTGATGCTCGCATTCCCCCCTTCCACGCACATA
CACAAACCCTTTCAGAAACGTCTACCAGATTCGAGACCCTGACTCTCTTC
CATGGGTTCTCGTCGAGACATGCGTTAGTTTCCATCAGCCGGGCAAGAAG
AGGCTGTCTCACCGCCTCCCCCGCCCATGGGGCGCTATGCGTGATCCGGA
ATAGCCTTCCAAGATGGTGTTACGAAAAACTGAAATATTTTGTGTAGGAT
GATGTTGGAAAAAATGAAATGTTTTGTACGGTACTAAATTGGCGAACAAA
ATTTCTCGTTTGCTTCTGCATGATGAAATGACGCTCGGGTGAAATGGCTG
GCAAACAAAAAATCAAAAGGTCGAAGATCGAAGGTCTGGAATCCTCTGTA
AAAGATATTTTCGCGGAAGAAAAGGTAAGGCGAGACAATTACGCGTAA
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Coding sequence (CDS) from alignment at H-elongata_contig66198:1108..2805+

>mRNA_H-elongata_contig66198.14213.1 ID=mRNA_H-elongata_contig66198.14213.1|Name=mRNA_H-elongata_contig66198.14213.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=534bp|location=Sequence derived from alignment at H-elongata_contig66198:1108..2805+ (Himanthalia elongata Himel1 dioecious)
ATGCCGCGCTCCGCGGATTCGTACATCCACCGAGTCGGCCGGACGGCGAG
GGCGGGCTGCGGAGGGTGCTCGGTGACACTTATCGATGCCGCGCTCCGCG
GATTCGTACATCCACCGAGTCGGCCGGACGGCGAGGGCGGGCTGCGGAGG
GTGCTCGGTGACACTTATCGGCGAAAGCAGACGGATAGTGATGAAAGAGG
TGCTAAAAACGCAGGGGTGCGAGGTGGAGATCAAGAGTAGGGCGGTCCCC
CAGTCGGCCATTGACCACTACAGGCGAAAGCAGACGGATAGTGATGAAAG
AGGTGCTAAAAACGCAGGGGTGCGAGGTGGAGATCAAGAGTAGGGCGGTC
CCCCAGTCGGCCATTGACCACTACAGGTCGAAGATCGAAGGTCTGGAATC
CTCTGTAAAAGATATTTTCGCGGAAGAAAAGGTAAGGCGAGACAATTACG
CGTAAGTCGAAGATCGAAGGTCTGGAATCCTCTGTAAAAGATATTTTCGC
GGAAGAAAAGGTAAGGCGAGACAATTACGCGTAA
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