mRNA_H-elongata_contig64529.14040.1 (mRNA) Himanthalia elongata Himel1 dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_H-elongata_contig64529.14040.1
Unique NamemRNA_H-elongata_contig64529.14040.1
TypemRNA
OrganismHimanthalia elongata Himel1 dioecious (Himanthalia elongata Himel1 dioecious)
Homology
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BLAST of mRNA_H-elongata_contig64529.14040.1 vs. uniprot
Match: D8LTF8_ECTSI (Steroid-5-alpha-reductase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LTF8_ECTSI)

HSP 1 Score: 130 bits (327), Expect = 9.340e-36
Identity = 59/74 (79.73%), Postives = 66/74 (89.19%), Query Frame = 1 
Query:   16 AGFLINYQSDGILRRLRGPGETGYKIPRGGMFEYISGANYFGECLEWCGFAIAGWSLPALAFALYTIANISPRA 237
            AG  IN QSDGILRRLR PGETGYKIP+GGMF Y+SGANYFGEC+ W GFAIAGWSLPA AFALYT++N++PRA
Sbjct:  162 AGMFINCQSDGILRRLRRPGETGYKIPQGGMFAYVSGANYFGECVGWIGFAIAGWSLPATAFALYTLSNLAPRA 235
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BLAST of mRNA_H-elongata_contig64529.14040.1 vs. uniprot
Match: A0A7R9YDD7_9STRA (Hypothetical protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9YDD7_9STRA)

HSP 1 Score: 121 bits (303), Expect = 3.000e-32
Identity = 57/76 (75.00%), Postives = 61/76 (80.26%), Query Frame = 1 
Query:   16 AGFLINYQSDGILRRLRGPGETGYKIPRGGMFEYISGANYFGECLEWCGFAIAGWSLPALAFALYTIANISPRAFQ 243
            AG LIN QSDGILR LR PGETGYKIPRGGMFEYISGA+YFGE LEW GFA+A W L A AFA +T  NI PRA+ 
Sbjct:  153 AGLLINVQSDGILRNLRKPGETGYKIPRGGMFEYISGAHYFGEILEWIGFAMASWRLRAAAFAFFTACNIGPRAYH 228
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BLAST of mRNA_H-elongata_contig64529.14040.1 vs. uniprot
Match: A0A1S3KCQ5_LINUN (3-oxo-5-alpha-steroid 4-dehydrogenase (NADP(+)) n=2 Tax=Lingula unguis TaxID=7574 RepID=A0A1S3KCQ5_LINUN)

HSP 1 Score: 120 bits (302), Expect = 7.020e-32
Identity = 54/75 (72.00%), Postives = 60/75 (80.00%), Query Frame = 1 
Query:   19 GFLINYQSDGILRRLRGPGETGYKIPRGGMFEYISGANYFGECLEWCGFAIAGWSLPALAFALYTIANISPRAFQ 243
            G  +N  SD +LR LR PGETGYKIPRGGMFEY+SGAN+FGECLEW GFAIA WSLPALAF ++T  N  PRAF 
Sbjct:  177 GMFVNIHSDHVLRNLRKPGETGYKIPRGGMFEYVSGANFFGECLEWTGFAIACWSLPALAFCIFTWLNTGPRAFH 251
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BLAST of mRNA_H-elongata_contig64529.14040.1 vs. uniprot
Match: A0A7S2XZJ2_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XZJ2_9STRA)

HSP 1 Score: 119 bits (299), Expect = 1.310e-31
Identity = 55/81 (67.90%), Postives = 62/81 (76.54%), Query Frame = 1 
Query:    1 IRLSIAGFLINYQSDGILRRLRGPGETGYKIPRGGMFEYISGANYFGECLEWCGFAIAGWSLPALAFALYTIANISPRAFQ 243
            I L +AGF IN  SDGILR+LR    +GYKIPRGGMFEY+SGAN+FGE +EW GF  AGWSLPA AFA +T  NI PRA Q
Sbjct:  152 ITLFLAGFAINMHSDGILRKLRTNNSSGYKIPRGGMFEYVSGANFFGEIVEWTGFTFAGWSLPATAFAFFTFCNIGPRAIQ 232
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BLAST of mRNA_H-elongata_contig64529.14040.1 vs. uniprot
Match: A0A6P4ZD89_BRABE (3-oxo-5-alpha-steroid 4-dehydrogenase (NADP(+)) n=1 Tax=Branchiostoma belcheri TaxID=7741 RepID=A0A6P4ZD89_BRABE)

HSP 1 Score: 119 bits (297), Expect = 3.590e-31
Identity = 55/81 (67.90%), Postives = 66/81 (81.48%), Query Frame = 1 
Query:    1 IRLSIAGFLINYQSDGILRRLRGPGETGYKIPRGGMFEYISGANYFGECLEWCGFAIAGWSLPALAFALYTIANISPRAFQ 243
            I L +AG +IN  +D ILR LR PGETGYKIP+GGMF Y+SGANYFGE +EW GFAIA WSLPA AFA++T +NI PRA++
Sbjct:  167 IVLFLAGMVINLHADHILRNLRKPGETGYKIPQGGMFTYVSGANYFGEIVEWTGFAIACWSLPAAAFAVFTASNIGPRAYR 247
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BLAST of mRNA_H-elongata_contig64529.14040.1 vs. uniprot
Match: A0A8B7YL18_ACAPL (3-oxo-5-alpha-steroid 4-dehydrogenase (NADP(+)) n=1 Tax=Acanthaster planci TaxID=133434 RepID=A0A8B7YL18_ACAPL)

HSP 1 Score: 119 bits (297), Expect = 3.750e-31
Identity = 54/79 (68.35%), Postives = 62/79 (78.48%), Query Frame = 1 
Query:    7 LSIAGFLINYQSDGILRRLRGPGETGYKIPRGGMFEYISGANYFGECLEWCGFAIAGWSLPALAFALYTIANISPRAFQ 243
            L  +G  IN  SD ILR LR PGETGYKIP GGMFEY+SGAN+FGE +EW GFAIA WSLP LAFA++T  NI PRA++
Sbjct:  171 LFFSGMAINIHSDSILRNLRKPGETGYKIPHGGMFEYVSGANFFGEAVEWSGFAIACWSLPGLAFAIFTACNIGPRAYR 249
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BLAST of mRNA_H-elongata_contig64529.14040.1 vs. uniprot
Match: UPI0018D62504 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like n=1 Tax=Patiria miniata TaxID=46514 RepID=UPI0018D62504)

HSP 1 Score: 119 bits (297), Expect = 3.750e-31
Identity = 54/79 (68.35%), Postives = 64/79 (81.01%), Query Frame = 1 
Query:    7 LSIAGFLINYQSDGILRRLRGPGETGYKIPRGGMFEYISGANYFGECLEWCGFAIAGWSLPALAFALYTIANISPRAFQ 243
            L   G  IN QSD ILR LR PGETGYKIP+GGMFEYISGAN+FGE +EW GFA+A WSLP+LAF ++T +NI PRA++
Sbjct:  171 LFFTGMAINIQSDSILRNLRKPGETGYKIPKGGMFEYISGANFFGEVVEWTGFALACWSLPSLAFLVFTASNIGPRAYR 249
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BLAST of mRNA_H-elongata_contig64529.14040.1 vs. uniprot
Match: UPI00071210C7 (3-oxo-5-alpha-steroid 4-dehydrogenase n=1 Tax=Blastocystis sp. subtype 4 TaxID=944170 RepID=UPI00071210C7)

HSP 1 Score: 118 bits (296), Expect = 4.130e-31
Identity = 55/79 (69.62%), Postives = 62/79 (78.48%), Query Frame = 1 
Query:    7 LSIAGFLINYQSDGILRRLRGPGETGYKIPRGGMFEYISGANYFGECLEWCGFAIAGWSLPALAFALYTIANISPRAFQ 243
            L IAG  IN+QSD ILR LR  G +GYKIP GGMFEY+SGANYFGEC+EW G+A   W+L ALAFA +TIANI PRA Q
Sbjct:  159 LFIAGLSINWQSDAILRSLRADGSSGYKIPHGGMFEYVSGANYFGECIEWIGYACLCWNLGALAFAFFTIANIGPRALQ 237
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BLAST of mRNA_H-elongata_contig64529.14040.1 vs. uniprot
Match: A0A497DW51_9BACT (3-oxo-5-alpha-steroid 4-dehydrogenase n=2 Tax=Candidatus Aminicenantes bacterium TaxID=2052149 RepID=A0A497DW51_9BACT)

HSP 1 Score: 117 bits (293), Expect = 9.460e-31
Identity = 52/77 (67.53%), Postives = 65/77 (84.42%), Query Frame = 1 
Query:    7 LSIAGFLINYQSDGILRRLRGPGETGYKIPRGGMFEYISGANYFGECLEWCGFAIAGWSLPALAFALYTIANISPRA 237
            L I GF++N  SD +LRRLR PGE GYKIPRGG+FEY+S ANYFGE +EW G+A+A WSLP LAFA++T+AN++PRA
Sbjct:  151 LFIGGFILNVYSDHLLRRLRKPGENGYKIPRGGLFEYVSCANYFGEIIEWFGWALATWSLPGLAFAIWTVANLAPRA 227
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BLAST of mRNA_H-elongata_contig64529.14040.1 vs. uniprot
Match: A0A2G9UF28_TELCI (3-oxo-5-alpha-steroid 4-dehydrogenase n=1 Tax=Teladorsagia circumcincta TaxID=45464 RepID=A0A2G9UF28_TELCI)

HSP 1 Score: 113 bits (283), Expect = 1.010e-30
Identity = 52/75 (69.33%), Postives = 59/75 (78.67%), Query Frame = 1 
Query:   19 GFLINYQSDGILRRLRGPGETGYKIPRGGMFEYISGANYFGECLEWCGFAIAGWSLPALAFALYTIANISPRAFQ 243
            G LIN  SD ILR LR PGE GYKIPRGGMFEYISGAN+FGE +EW GFA+   SLPA++FA++T  NI PRA Q
Sbjct:   26 GMLINIHSDSILRNLRKPGEVGYKIPRGGMFEYISGANFFGEIVEWTGFAVMSGSLPAISFAIFTACNIGPRAIQ 100
The following BLAST results are available for this feature:
BLAST of mRNA_H-elongata_contig64529.14040.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LTF8_ECTSI9.340e-3679.73Steroid-5-alpha-reductase n=1 Tax=Ectocarpus silic... [more]
A0A7R9YDD7_9STRA3.000e-3275.00Hypothetical protein n=1 Tax=Pinguiococcus pyrenoi... [more]
A0A1S3KCQ5_LINUN7.020e-3272.003-oxo-5-alpha-steroid 4-dehydrogenase (NADP(+)) n=... [more]
A0A7S2XZJ2_9STRA1.310e-3167.90Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A6P4ZD89_BRABE3.590e-3167.903-oxo-5-alpha-steroid 4-dehydrogenase (NADP(+)) n=... [more]
A0A8B7YL18_ACAPL3.750e-3168.353-oxo-5-alpha-steroid 4-dehydrogenase (NADP(+)) n=... [more]
UPI0018D625043.750e-3168.353-oxo-5-alpha-steroid 4-dehydrogenase 1-like n=1 T... [more]
UPI00071210C74.130e-3169.623-oxo-5-alpha-steroid 4-dehydrogenase n=1 Tax=Blas... [more]
A0A497DW51_9BACT9.460e-3167.533-oxo-5-alpha-steroid 4-dehydrogenase n=2 Tax=Cand... [more]
A0A2G9UF28_TELCI1.010e-3069.333-oxo-5-alpha-steroid 4-dehydrogenase n=1 Tax=Tela... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
H-elongata_contig64529contigH-elongata_contig64529:1786..3205 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Himanthalia elongata Himel1 dioecious2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score132.1
Seed ortholog evalue1.2e-28
Seed eggNOG ortholog2880.D8LTF8
Preferred nameSRD5A1
KEGG rclassRC00145
KEGG koko:K09591,ko:K10901,ko:K12343,ko:K12344,ko:K14570
KEGG ReactionR02208,R02497,R07429,R07447,R08954,R10242
KEGG Pathwayko00140,ko00905,ko01100,ko01110,ko03008,ko03440,ko03460,ko05215,map00140,map00905,map01100,map01110,map03008,map03440,map03460,map05215
KEGG ModuleM00295,M00371,M00414
GOsGO:0000003,GO:0000166,GO:0001501,GO:0001505,GO:0001655,GO:0001889,GO:0003006,GO:0003674,GO:0003824,GO:0003865,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0006082,GO:0006091,GO:0006629,GO:0006694,GO:0006702,GO:0006706,GO:0006720,GO:0006721,GO:0006725,GO:0006805,GO:0006807,GO:0006950,GO:0007154,GO:0007267,GO:0007275,GO:0007399,GO:0007417,GO:0007420,GO:0007530,GO:0007548,GO:0007610,GO:0007622,GO:0007623,GO:0008150,GO:0008152,GO:0008202,GO:0008207,GO:0008209,GO:0008406,GO:0008584,GO:0008610,GO:0009055,GO:0009056,GO:0009058,GO:0009267,GO:0009410,GO:0009605,GO:0009636,GO:0009719,GO:0009725,GO:0009917,GO:0009987,GO:0009991,GO:0010033,GO:0010243,GO:0010268,GO:0010817,GO:0012505,GO:0014070,GO:0014074,GO:0014823,GO:0014850,GO:0016020,GO:0016042,GO:0016101,GO:0016128,GO:0016129,GO:0016131,GO:0016132,GO:0016229,GO:0016491,GO:0016614,GO:0016616,GO:0016627,GO:0016628,GO:0016999,GO:0017144,GO:0018879,GO:0018894,GO:0018958,GO:0018963,GO:0019752,GO:0021510,GO:0021536,GO:0021537,GO:0021543,GO:0021761,GO:0021766,GO:0021794,GO:0021854,GO:0021983,GO:0021987,GO:0022410,GO:0022414,GO:0022900,GO:0023052,GO:0030283,GO:0030431,GO:0030539,GO:0030540,GO:0030900,GO:0031667,GO:0031668,GO:0031669,GO:0031960,GO:0031984,GO:0032354,GO:0032355,GO:0032501,GO:0032502,GO:0032868,GO:0032869,GO:0032870,GO:0033218,GO:0033554,GO:0033574,GO:0033764,GO:0033765,GO:0033993,GO:0034641,GO:0034698,GO:0034754,GO:0035270,GO:0035690,GO:0036094,GO:0036477,GO:0042133,GO:0042175,GO:0042180,GO:0042181,GO:0042221,GO:0042428,GO:0042430,GO:0042445,GO:0042446,GO:0042448,GO:0042493,GO:0042537,GO:0042592,GO:0042594,GO:0042745,GO:0042747,GO:0042995,GO:0043005,GO:0043025,GO:0043167,GO:0043168,GO:0043209,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043434,GO:0043436,GO:0043627,GO:0043648,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044281,GO:0044283,GO:0044297,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0045137,GO:0046483,GO:0046546,GO:0046660,GO:0046661,GO:0046677,GO:0046683,GO:0048037,GO:0048471,GO:0048511,GO:0048512,GO:0048513,GO:0048545,GO:0048608,GO:0048731,GO:0048732,GO:0048806,GO:0048856,GO:0048878,GO:0050213,GO:0050661,GO:0050662,GO:0050802,GO:0050896,GO:0051384,GO:0051591,GO:0051716,GO:0055088,GO:0055114,GO:0060322,GO:0060348,GO:0060416,GO:0060992,GO:0061008,GO:0061370,GO:0061458,GO:0065007,GO:0065008,GO:0070402,GO:0070848,GO:0070852,GO:0070887,GO:0071310,GO:0071320,GO:0071363,GO:0071375,GO:0071383,GO:0071384,GO:0071385,GO:0071392,GO:0071394,GO:0071396,GO:0071407,GO:0071417,GO:0071466,GO:0071495,GO:0071496,GO:0071548,GO:0071549,GO:0071704,GO:0071867,GO:0071868,GO:0071869,GO:0071870,GO:0071871,GO:0071872,GO:0097159,GO:0097164,GO:0097327,GO:0097458,GO:0098827,GO:0120025,GO:1901160,GO:1901265,GO:1901360,GO:1901361,GO:1901362,GO:1901363,GO:1901564,GO:1901575,GO:1901576,GO:1901615,GO:1901617,GO:1901652,GO:1901653,GO:1901654,GO:1901655,GO:1901698,GO:1901699,GO:1901700,GO:1901701
EggNOG free text desc.3-oxo-5-alpha-steroid 4-dehydrogenase activity
EggNOG OGsKOG1638@1,KOG1638@2759
EC1.3.1.22,3.6.4.12
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03009,ko03032,ko03400
Hectar predicted targeting categoryother localisation
Exons3
Model size252
Cds size252
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_H-elongata_contig64529.14040.1prot_H-elongata_contig64529.14040.1Himanthalia elongata Himel1 dioeciouspolypeptideH-elongata_contig64529 1786..3205 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931031.3277912-CDS-H-elongata_contig64529:1785..18851622931031.3277912-CDS-H-elongata_contig64529:1785..1885Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig64529 1786..1885 +
1691679669.2902665-CDS-H-elongata_contig64529:1785..18851691679669.2902665-CDS-H-elongata_contig64529:1785..1885Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig64529 1786..1885 +
1622931031.339591-CDS-H-elongata_contig64529:2516..25771622931031.339591-CDS-H-elongata_contig64529:2516..2577Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig64529 2517..2577 +
1691679669.30644-CDS-H-elongata_contig64529:2516..25771691679669.30644-CDS-H-elongata_contig64529:2516..2577Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig64529 2517..2577 +
1622931031.3590617-CDS-H-elongata_contig64529:3114..32051622931031.3590617-CDS-H-elongata_contig64529:3114..3205Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig64529 3115..3205 +
1691679669.3210971-CDS-H-elongata_contig64529:3114..32051691679669.3210971-CDS-H-elongata_contig64529:3114..3205Himanthalia elongata Himel1 dioeciousCDSH-elongata_contig64529 3115..3205 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_H-elongata_contig64529.14040.1

>prot_H-elongata_contig64529.14040.1 ID=prot_H-elongata_contig64529.14040.1|Name=mRNA_H-elongata_contig64529.14040.1|organism=Himanthalia elongata Himel1 dioecious|type=polypeptide|length=84bp
IRLSIAGFLINYQSDGILRRLRGPGETGYKIPRGGMFEYISGANYFGECL
EWCGFAIAGWSLPALAFALYTIANISPRAFQVL*
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mRNA from alignment at H-elongata_contig64529:1786..3205+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_H-elongata_contig64529.14040.1 ID=mRNA_H-elongata_contig64529.14040.1|Name=mRNA_H-elongata_contig64529.14040.1|organism=Himanthalia elongata Himel1 dioecious|type=mRNA|length=1420bp|location=Sequence derived from alignment at H-elongata_contig64529:1786..3205+ (Himanthalia elongata Himel1 dioecious)
ATACGTTTATCAATCGCAGGGTTTCTCATAAATTACCAATCCGATGGGAT
TTTGAGGCGGCTGCGTGGGCCTGGAGAAACTGGCTACAAGATCCCCAGAG
GTAGAAAACTGCAATGCTACAGTACGGTACTGTAATTGTTTAGGCAAAAT
GTGATGCGCGTAGAGCACGCTGGCATCAAGCTCAGTTCTTTGTTCCACCC
TCTTCTGGAATTTAGAGAAGCCGTGTTTGCGACTGTGTAGACTATGCCAG
GCATGAGTCTCTCCGTTTATTATTTTTGTTATTATTATTATTATTATCAT
TATTATTATTATTATTATTATTATCATTTAAAAAATCATGAACTCTTTCC
AATCCTCCGAACATCCACGCCTTCTTAGTACCGTAAGAGGTGTAGTCCAG
GGGACCAACTATGATATCGTCAGGTTCGAATGTGGACATTTTAGCTCCAT
TTAAGAACAAAATCGTCGCATGTGATTGATCGTGTTCCCCAACAGTAAAT
TCATCGGGTCAACGGTGTAATGACGAGGATCCCATCATTATCCAGTACAC
TATTCCCGCCGCCTCGTCCTATCCGCGTTGTTACCATCCAGCCTTTTCTA
CATTACACACTTTTGATTTCCACATATTCCTAACCCTATGCACATACATA
TTATTACATGCCTTTTCTTTGTACCGGTGTGTCGCCTTTTGTGTCGATTG
TCGCTGTGCTAATGTTAAACACATCAATCAGGCGGCATGTTTGAATATAT
CTCCGGGGCTAACTACTTCGGAGAGTGCCTGGAGTGGTGCGGGTGCGTCG
TTAAGTTGCGGATGAACTTGAGTATTTATATAAAGTTCTCGTCATGCATT
ATTACCTCCATGTACCCATTCCATCCCCTTCGATTCGCCGGGGCGAGACT
CAATTTGAGCATTGATTGGATTTGAGTATGCGTGTTGGCAGCAGTACATT
TTGTACTTCCCCTGTCTCGGCACGTCTGCCGACAAGTAAGACCGCCCGAC
GAGATAAATGACCATAGACGAGTACATCTTTGAGAATTGTCCACACATCC
GCCGTATTGGCAACAACTGCACGAAGTCTTCGTTGGGGTGAATTGATAGC
TTCCTCTCTGCTCCTACAGTATTCCATCCTACGCAGAGGTTAGCTCAACA
ATCGAGTATTAGTACTAATAGTACTCAATTATAACACGAACGGTGCCCCC
TTGCACGCCGCGCGATTTCAACACGTATGACCTTTCGTACCCCCTTGTGC
ACCCCCCCCCACTGCCCGGCCCACGATTTCGCTTTAATTTTTTCGTGGCA
CATCTCATTGTTTGGGTCGTCGCCCGAAGTTTCGCGATCGCGGGCTGGTC
GTTGCCGGCCCTCGCTTTTGCTCTCTACACCATCGCCAACATATCTCCGC
GGGCCTTTCAGGTACTGTAG
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Coding sequence (CDS) from alignment at H-elongata_contig64529:1786..3205+

>mRNA_H-elongata_contig64529.14040.1 ID=mRNA_H-elongata_contig64529.14040.1|Name=mRNA_H-elongata_contig64529.14040.1|organism=Himanthalia elongata Himel1 dioecious|type=CDS|length=504bp|location=Sequence derived from alignment at H-elongata_contig64529:1786..3205+ (Himanthalia elongata Himel1 dioecious)
ATACGTTTATCAATCGCAGGGTTTCTCATAAATTACCAATCCGATGGGAT
TTTGAGGCGGCTGCGTGGGCCTGGAGAAACTGGCTACAAGATCCCCAGAG
ATACGTTTATCAATCGCAGGGTTTCTCATAAATTACCAATCCGATGGGAT
TTTGAGGCGGCTGCGTGGGCCTGGAGAAACTGGCTACAAGATCCCCAGAG
GCGGCATGTTTGAATATATCTCCGGGGCTAACTACTTCGGAGAGTGCCTG
GAGTGGTGCGGGCGGCATGTTTGAATATATCTCCGGGGCTAACTACTTCG
GAGAGTGCCTGGAGTGGTGCGGTTTCGCGATCGCGGGCTGGTCGTTGCCG
GCCCTCGCTTTTGCTCTCTACACCATCGCCAACATATCTCCGCGGGCCTT
TCAGGTACTGTAGTTTCGCGATCGCGGGCTGGTCGTTGCCGGCCCTCGCT
TTTGCTCTCTACACCATCGCCAACATATCTCCGCGGGCCTTTCAGGTACT
GTAG
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